CN114009586A - Development and application of composite probiotic preparation with high intestinal tract colonization rate - Google Patents
Development and application of composite probiotic preparation with high intestinal tract colonization rate Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/165—Paracasei
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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Abstract
The invention discloses development and application of a group of compound probiotic preparations with high intestinal colonization rate, wherein the compound probiotic preparations comprise the following components: the multifunctional compound probiotic preparation comprises a digestion promoting Chinese herbal medicine extracting solution, molasses, bran, carbamide, compound amino acid, compound trace elements, compound vitamins and distilled water; the invention adopts the multifunctional composite probiotics consisting of the salt-tolerant composite probiotic strains and the acid-tolerant composite probiotic strains to improve the salt tolerance and the acid tolerance of the composite probiotics, thereby effectively resisting the erosion of gastric acid and bile salt in intestinal tracts.
Description
Technical Field
The invention relates to composite probiotics, in particular to development and application of a group of composite probiotic preparations with high intestinal colonization rate, and belongs to the technical field of probiotics.
Background
Probiotics are active microorganisms which are beneficial to a host and change the composition of flora at a certain part of the host by colonizing in a human body. The health of the intestinal tract is kept by adjusting the immune function of host mucous membrane and system or adjusting the flora balance in the intestinal tract, so that single microorganism or mixed microorganism with definite composition beneficial to health is generated, beneficial bacteria or fungi in human bodies and animal bodies mainly comprise saccharomycetes, probiotic bacillus, clostridium butyricum, lactobacillus, bifidobacteria, actinomycetes and the like, and the saccharomycetes comprise: at present, researches show that the yeast has good probiotic characteristics of regulating intestinal balance, promoting feed conversion, improving the immune function of organisms and the like, and is mostly used as a feed additive for livestock and poultry breeding. The most studied yeasts are Saccharomyces, Torulopsis, Candida, Willemm, Pichia, Saccharomyces, Torulopsis, Schoeckera, Rhodotorula, Schizosaccharomyces pombe, Saccharomyces baumannii, Bacillus: is a group of aerobic or facultative anaerobic, spore-forming rod-shaped bacteria with probiotic properties to the body, is a gram-positive bacterium, lactic acid bacteria are the most common probiotics, have been widely used and are considered safe to humans and animals.
The probiotics can reach the intestinal tract to take effect after passing through a heavy check from the stomach and the duodenum to the small intestine and the large intestine, and the heavy check of gastric acid and bile is needed.
Disclosure of Invention
The invention aims to provide development and application of a group of compound probiotic preparations with high intestinal colonization rate so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: development and application of a group of compound probiotic preparations with high intestinal colonization rate, wherein the compound probiotic preparations comprise the following components:
the multifunctional composite probiotic preparation comprises a multifunctional composite probiotic preparation, digestion promoting Chinese herbal medicine extracting solution, molasses, bran, carbamide, composite amino acid, composite trace elements, composite vitamins and distilled water.
As a preferred technical scheme of the invention, the probiotic preparation comprises the following components in parts by weight:
10-15 parts of multifunctional composite probiotic preparation, 3-6 parts of digestion promoting Chinese herbal medicine extract, 2-5 parts of molasses, 1-2 parts of bran, 0.1-0.5 part of carbamide, 0.1-0.3 part of compound amino acid, 0.1-0.5 part of compound trace element, 0.5-1.5 part of compound vitamin and 80-100 parts of water.
As a preferred technical scheme of the invention, the multifunctional compound probiotic preparation consists of 3-5 mass components of salt-resistant compound probiotic strain and 8-10 mass components of acid-resistant compound probiotic strain.
As a preferred technical scheme, the salt-tolerant composite probiotic strain comprises Saccharomyces rouxii, Bacillus subtilis, Rhodopseudomonas palustris, halophilic actinomycetes, Lactobacillus casei, Paenibacillus polymyxa and Saccharomyces cerevisiae.
As a preferred technical scheme, in the salt-tolerant composite probiotic strain, according to the mass components, the content of the saccharomyces rouxii is 2 components, the content of the bacillus subtilis is 2 components, the content of the rhodopseudomonas palustris is 4 components, the content of the halophilic actinomycetes is 2 components, the content of the lactobacillus casei is 4 components, the content of the paenibacillus polymyxa is 4 components, and the content of the saccharomyces cerevisiae is 4 components.
As a preferred technical scheme of the invention, the acid-resistant composite probiotic strains comprise bacillus coagulans, enterococcus faecium, lactobacillus paracasei, pediococcus acidilactici, bifidobacterium animalis BB-12 and lactobacillus acidophilus LA-5.
As a preferred technical scheme of the invention, the acid-resistant composite probiotic strain contains 2-4 parts of bacillus coagulans, 1-3 parts of enterococcus faecium, 1-3 parts of lactobacillus paracasei, 1-2 parts of pediococcus acidilactici, BB-122-4 parts of bifidobacterium animalis and LA-51-2 parts of lactobacillus acidophilus.
As a preferred technical scheme, the Chinese herbal medicine extracting solution comprises a hawthorn extracting solution, a malt extracting solution, a medicated leaven extracting solution, a chicken's gizzard-membrane extracting solution, a immature bitter orange extracting solution, a rice sprout extracting solution and a hammer leaf extracting solution.
Compared with the prior art, the invention has the beneficial effects that:
the invention discloses development and application of a group of compound probiotic preparations with high intestinal colonization rate, wherein multifunctional compound probiotics consisting of salt-tolerant compound probiotic strains and acid-tolerant compound probiotic strains are adopted to improve the salt tolerance and acid tolerance of the compound probiotics, so that the corrosion of gastric acid and bile salt in intestinal tracts is effectively resisted, and meanwhile, Chinese herbal medicine extracting solutions consisting of hawthorn extracting solution, malt extracting solution, medicated leaven extracting solution, endothelium corneum gigeriae galli extracting solution, immature bitter orange extracting solution, rice sprout extracting solution and hammer piece extracting solution are added while the strains with multiple tolerance functions are adopted, so that the digestion is further promoted, and the intestinal colonization rate is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: the invention provides a technical scheme for developing and applying a group of composite probiotic preparations with high intestinal colonization rate, which comprises the following steps: development and application of a group of composite probiotic preparations with high intestinal colonization rate, wherein the probiotic preparations comprise the following components in parts by mass:
the multifunctional composite probiotic preparation comprises a component 10, a digestion-promoting Chinese herbal medicine extract 3, a molasses 2, a bran 1, a carbamide 0.1, a composite amino acid 0.1, a composite trace element 0.1, a composite vitamin 0.5 and distilled water 80.
Specifically, the multifunctional composite probiotic preparation comprises 3 mass components of salt-tolerant composite probiotic strains and 8 mass components of acid-resistant composite probiotic strains, wherein the salt-tolerant composite probiotic strains comprise 2 mass components of Saccharomyces rouxii, Bacillus subtilis, Rhodopseudomonas palustris, halophilic actinomycetes, Lactobacillus casei, Paenibacillus polymyxa and Saccharomyces cerevisiae, and in the salt-tolerant composite probiotic strains, according to the mass components, the content of the Saccharomyces rouxii is 2 mass components, the content of the Bacillus subtilis is 2 mass components, the content of the Rhodopseudomonas palustris is 4 mass components, the content of the halophilic actinomycetes is 2 mass components, the content of the Lactobacillus casei is 4 mass components, the content of the Paenibacillus polymyxa is 4 mass components, and the content of the Saccharomyces cerevisiae is 4 mass components.
The Chinese herbal medicine extractive solution comprises fructus crataegi extractive solution, fructus Hordei Germinatus extractive solution, Massa Medicata Fermentata extractive solution, endothelium corneum Gigeriae Galli extractive solution, fructus Aurantii Immaturus extractive solution, fructus Setariae Germinatus extractive solution and Arecae semen extractive solution.
The acid-resistant composite probiotic strain comprises bacillus coagulans, enterococcus faecium, lactobacillus paracasei, pediococcus acidilactici, bifidobacterium animalis BB-12 and lactobacillus acidophilus LA-5, and contains 2-4 parts of bacillus coagulans, 1-3 parts of enterococcus faecium, 1-3 parts of lactobacillus paracasei, 1-2 parts of pediococcus acidilactici, BB-122-4 parts of bifidobacterium animalis and LA-51-2 parts of lactobacillus acidophilus.
Example two: the invention provides a technical scheme for developing and applying a group of composite probiotic preparations with high intestinal colonization rate, which comprises the following steps: development and application of a group of composite probiotic preparations with high intestinal colonization rate, wherein the probiotic preparations comprise the following components in parts by mass:
12 components of a multifunctional compound probiotic preparation, 5 components of digestion promoting Chinese herbal medicine extract, 4 components of molasses, 2 components of bran, 0.3 component of carbamide, 0.2 component of compound amino acid, 0.3 component of compound trace element, 1 component of compound vitamin and 80 components of distilled water.
Specifically, the multifunctional composite probiotic preparation comprises salt-tolerant composite probiotic strains with 4 mass components and acid-resistant composite probiotic strains with 9 mass components, wherein the salt-tolerant composite probiotic strains comprise 2 mass components of Saccharomyces rouxii, Bacillus subtilis, Rhodopseudomonas palustris, halophilic actinomycetes, Lactobacillus casei, Paenibacillus polymyxa and Saccharomyces cerevisiae, and in the salt-tolerant composite probiotic strains, according to the mass components, the content of the Saccharomyces rouxii is 2 mass components, the content of the Bacillus subtilis is 2 mass components, the content of the Rhodopseudomonas palustris is 4 mass components, the content of the halophilic actinomycetes is 2 mass components, the content of the Lactobacillus casei is 4 mass components, the content of the Paenibacillus polymyxa is 4 mass components, and the content of the Saccharomyces cerevisiae is 4 mass components.
The Chinese herbal medicine extractive solution comprises fructus crataegi extractive solution, fructus Hordei Germinatus extractive solution, Massa Medicata Fermentata extractive solution, endothelium corneum Gigeriae Galli extractive solution, fructus Aurantii Immaturus extractive solution, fructus Setariae Germinatus extractive solution and Arecae semen extractive solution.
The acid-resistant composite probiotic strain comprises bacillus coagulans, enterococcus faecium, lactobacillus paracasei, pediococcus acidilactici, bifidobacterium animalis BB-12 and lactobacillus acidophilus LA-5, and contains 2-4 parts of bacillus coagulans, 1-3 parts of enterococcus faecium, 1-3 parts of lactobacillus paracasei, 1-2 parts of pediococcus acidilactici, BB-122-4 parts of bifidobacterium animalis and LA-51-2 parts of lactobacillus acidophilus.
Example three: the invention provides a technical scheme for developing and applying a group of composite probiotic preparations with high intestinal colonization rate, which comprises the following steps: development and application of a group of composite probiotic preparations with high intestinal colonization rate, wherein the probiotic preparations comprise the following components in parts by mass:
15 components of a multifunctional compound probiotic preparation, 6 components of digestion promoting Chinese herbal medicine extract, 5 components of molasses, 2 components of bran, 0.5 component of carbamide, 0.3 component of compound amino acid, 0.5 component of compound trace element, 1.5 components of compound vitamin and 100 components of distilled water.
Specifically, the multifunctional composite probiotic preparation comprises 5 mass components of salt-tolerant composite probiotic strains and 10 mass components of acid-resistant composite probiotic strains, wherein the salt-tolerant composite probiotic strains comprise 2 mass components of Saccharomyces rouxii, Bacillus subtilis, Rhodopseudomonas palustris, halophilic actinomycetes, Lactobacillus casei, Paenibacillus polymyxa and Saccharomyces cerevisiae, and in the salt-tolerant composite probiotic strains, according to the mass components, the content of the Saccharomyces rouxii is 2 mass components, the content of the Bacillus subtilis is 2 mass components, the content of the Rhodopseudomonas palustris is 4 mass components, the content of the halophilic actinomycetes is 2 mass components, the content of the Lactobacillus casei is 4 mass components, the content of the Paenibacillus polymyxa is 4 mass components, and the content of the Saccharomyces cerevisiae is 4 mass components.
The Chinese herbal medicine extractive solution comprises fructus crataegi extractive solution, fructus Hordei Germinatus extractive solution, Massa Medicata Fermentata extractive solution, endothelium corneum Gigeriae Galli extractive solution, fructus Aurantii Immaturus extractive solution, fructus Setariae Germinatus extractive solution and Arecae semen extractive solution.
The acid-resistant composite probiotic strain comprises bacillus coagulans, enterococcus faecium, lactobacillus paracasei, pediococcus acidilactici, bifidobacterium animalis BB-12 and lactobacillus acidophilus LA-5, and contains 2-4 parts of bacillus coagulans, 1-3 parts of enterococcus faecium, 1-3 parts of lactobacillus paracasei, 1-2 parts of pediococcus acidilactici, BB-122-4 parts of bifidobacterium animalis and LA-51-2 parts of lactobacillus acidophilus.
When the compound probiotic preparation is used specifically, the multifunctional compound probiotic consisting of the salt-tolerant compound probiotic strain and the acid-resistant compound probiotic strain is adopted to improve the salt tolerance and the acid tolerance of the compound probiotic, so that the erosion of gastric acid and bile salt in the intestinal tract is effectively resisted, and meanwhile, the Chinese herbal medicine extracting solution consisting of the hawthorn extracting solution, the malt extracting solution, the medicated leaven extracting solution, the endothelium corneum gigeriae galli extracting solution, the immature bitter orange extracting solution, the rice sprout extracting solution and the hammer leaf extracting solution is added while the multi-tolerance strain is adopted, so that the digestion is further promoted, and the intestinal colonization rate is improved.
In the description of the present invention, it is to be understood that the indicated orientations or positional relationships are only for convenience in describing the present invention and for simplicity in description, and are not intended to indicate or imply that the indicated devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be construed as limiting the present invention.
In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly attached, detachably attached, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. Development and application of a group of compound probiotic preparations with high intestinal colonization rate are characterized in that the compound probiotic preparations comprise the following components:
the multifunctional composite probiotic preparation comprises a multifunctional composite probiotic preparation, digestion promoting Chinese herbal medicine extracting solution, molasses, bran, carbamide, composite amino acid, composite trace elements, composite vitamins and distilled water.
2. The development and application of a group of composite probiotic preparations with high intestinal colonization rate according to claim 1 are characterized in that: the probiotic preparation comprises the following components in parts by weight:
10-15 parts of multifunctional composite probiotic preparation, 3-6 parts of digestion promoting Chinese herbal medicine extract, 2-5 parts of molasses, 1-2 parts of bran, 0.1-0.5 part of carbamide, 0.1-0.3 part of compound amino acid, 0.1-0.5 part of compound trace element, 0.5-1.5 part of compound vitamin and 80-100 parts of water.
3. The development and application of a group of composite probiotic preparations with high intestinal colonization rate according to claim 1 are characterized in that: the multifunctional composite probiotic preparation consists of 3-5 mass components of salt-resistant composite probiotic strains and 8-10 mass components of acid-resistant composite probiotic strains.
4. The development and application of a group of composite probiotic preparations with high intestinal colonization rate according to claim 1 are characterized in that: the salt-tolerant composite probiotic strain comprises Saccharomyces rouxii, Bacillus subtilis, Rhodopseudomonas palustris, halophilic actinomycetes, Lactobacillus casei, Paenibacillus polymyxa and Saccharomyces cerevisiae.
5. The development and application of a group of composite probiotic preparations with high intestinal colonization rate according to claim 4 are characterized in that: in the salt-tolerant composite probiotic strain, according to the mass components, the content of the saccharomyces rouxii is 2 components, the content of the bacillus subtilis is 2 components, the content of the rhodopseudomonas palustris is 4 components, the content of the halophilic actinomycetes is 2 components, the content of the lactobacillus casei is 4 components, the content of the paenibacillus polymyxa is 4 components, and the content of the saccharomyces cerevisiae is 4 components.
6. The development and application of a group of composite probiotic preparations with high intestinal colonization rate according to claim 1 are characterized in that: the acid-resistant composite probiotic strain comprises bacillus coagulans, enterococcus faecium, lactobacillus paracasei, pediococcus acidilactici, bifidobacterium animalis BB-12 and lactobacillus acidophilus LA-5.
7. The development and application of a group of composite probiotic preparations with high intestinal colonization rate according to claim 6 are characterized in that: the acid-resistant composite probiotic strain contains 2-4 parts of bacillus coagulans, 1-3 parts of enterococcus faecium, 1-3 parts of lactobacillus paracasei, 1-2 parts of pediococcus acidilactici, BB-122-4 parts of bifidobacterium animalis and LA-51-2 parts of lactobacillus acidophilus.
8. The development and application of a group of composite probiotic preparations with high intestinal colonization rate according to claim 1 are characterized in that: the Chinese herbal medicine extract comprises fructus crataegi extract, fructus Hordei Germinatus extract, Massa Medicata Fermentata extract, endothelium corneum Gigeriae Galli extract, fructus Aurantii Immaturus extract, fructus Setariae Germinatus extract and semen Arecae extract.
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| CN110623984A (en) * | 2019-10-22 | 2019-12-31 | 江苏恒丰强生物技术有限公司 | Quadruple viable bacteria preparation for treating diarrhea and application thereof |
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| CN110623984A (en) * | 2019-10-22 | 2019-12-31 | 江苏恒丰强生物技术有限公司 | Quadruple viable bacteria preparation for treating diarrhea and application thereof |
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