CN114009584A - Preparation method of additive for promoting growth and development of intestinal structures of young animals - Google Patents
Preparation method of additive for promoting growth and development of intestinal structures of young animals Download PDFInfo
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- CN114009584A CN114009584A CN202011405318.XA CN202011405318A CN114009584A CN 114009584 A CN114009584 A CN 114009584A CN 202011405318 A CN202011405318 A CN 202011405318A CN 114009584 A CN114009584 A CN 114009584A
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- 239000000654 additive Substances 0.000 title claims abstract description 22
- 230000000996 additive effect Effects 0.000 title claims abstract description 21
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 20
- 241001465754 Metazoa Species 0.000 title claims abstract description 14
- 238000011161 development Methods 0.000 title claims abstract description 14
- 230000001737 promoting effect Effects 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000006041 probiotic Substances 0.000 claims abstract description 55
- 235000018291 probiotics Nutrition 0.000 claims abstract description 55
- 239000002207 metabolite Substances 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 16
- 235000019722 synbiotics Nutrition 0.000 claims abstract description 16
- 108010016626 Dipeptides Proteins 0.000 claims abstract description 14
- 125000004122 cyclic group Chemical group 0.000 claims abstract description 14
- 230000000529 probiotic effect Effects 0.000 claims abstract description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 10
- 238000009395 breeding Methods 0.000 claims abstract description 7
- 230000001488 breeding effect Effects 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims abstract description 5
- 230000004151 fermentation Effects 0.000 claims abstract description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 5
- 238000013329 compounding Methods 0.000 claims abstract description 4
- 229940066544 lactobacillus sporogenes Drugs 0.000 claims abstract description 4
- 230000002503 metabolic effect Effects 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 238000000746 purification Methods 0.000 claims abstract description 4
- 239000008247 solid mixture Substances 0.000 claims abstract description 4
- 230000009286 beneficial effect Effects 0.000 claims abstract description 3
- 238000000926 separation method Methods 0.000 claims abstract description 3
- 238000011282 treatment Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005728 strengthening Methods 0.000 abstract description 5
- 210000000936 intestine Anatomy 0.000 abstract description 2
- 241000287828 Gallus gallus Species 0.000 description 15
- 235000013330 chicken meat Nutrition 0.000 description 13
- 235000013601 eggs Nutrition 0.000 description 9
- 210000001035 gastrointestinal tract Anatomy 0.000 description 8
- 238000005303 weighing Methods 0.000 description 6
- 241000607142 Salmonella Species 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000002159 abnormal effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000003746 feather Anatomy 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 208000027312 Bursal disease Diseases 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000004347 intestinal mucosa Anatomy 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 102000044503 Antimicrobial Peptides Human genes 0.000 description 1
- 108700042778 Antimicrobial Peptides Proteins 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- -1 microalgae Chemical class 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
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Abstract
The invention discloses a preparation method of an additive for promoting the growth and development of intestinal structures of young animals, which comprises the following steps: screening and culturing specific probiotic strains with the metabolic cyclic dipeptide functional peptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003; step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and liquid synbiotics of the probiotics and the metabolite are prepared; step three: solid-state treatment, separation and purification to obtain a solid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and preparing powder synbiotics of the probiotics and the metabolite; step four: screening prebiotics which are beneficial to the growth and the breeding of the specific probiotic strains and have good stability; step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive. The invention has the following advantages: the finished product is rich in specific prebiotics, specific probiotics and specific prebiotics, and has a mixed type probiotic with intestine strengthening function and outstanding effect.
Description
Technical Field
The invention relates to the technical field of intestinal tract structure growth and development of young animals, in particular to a preparation method of an additive for promoting the intestinal tract structure growth and development of the young animals.
Background
In the animal intestinal probiotics family, the probiotics which play a positive role in the intestinal tract not only comprise the probiotics, but also comprise food-prebiotics of the probiotics and metabolites of the probiotics, some probiotics have a synergistic effect with other probiotics, the products of some probiotics are food of other probiotics, and the mutual synergy exists among the prebiotics and the probiotics, so that the synergy among the probiotics is researched, the intestinal probiotics can be strengthened to play a role in strengthening the intestinal tract, and the effect of 1+1 is more than 2.
From the food of the probiotics, namely prebiotics, to the probiotics and then to the metabolite of the probiotics, namely prebiotics, the more definite and powerful the functionality, for example, enzyme preparations, antimicrobial peptides, cyclic dipeptides, vitamins, trace elements and the like which are the metabolites of the probiotics have definite functionality.
Based on a certain probiotic with definite functions, the core of the invention technology is to find out the probiotics capable of producing the metabolite, culture, breed and expand the flora to obtain the metabolite, find out the prebiotics capable of promoting the reproduction and metabolism of the flora and play the synergistic effect of a specific probiotic system.
Disclosure of Invention
In order to solve the technical problems, the technical scheme provided by the invention is as follows: a preparation method of an additive for promoting the growth and development of intestinal structures of young animals comprises the following steps:
the method comprises the following steps: screening and culturing specific probiotic strains with the metabolic cyclic dipeptide functional peptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003;
step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and liquid synbiotics of the probiotics and the metabolite are prepared;
step three: solid-state treatment, separation and purification to obtain a solid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and preparing powder synbiotics of the probiotics and the metabolite;
step four: screening prebiotics which are beneficial to the growth and the breeding of the specific probiotic strains and have good stability;
step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive.
After the steps are adopted, the invention has the following advantages: the invention prepares a mixed type probiotic rich in specific prebiotics, specific probiotics and specific probiotics through the processes of screening, culturing, fermenting, solidifying, purifying, mixing and the like, so that the mixed type probiotic has a clear intestine-strengthening function and a remarkable effect, aims to strengthen the clear functionality of the probiotics, enables the intestine-strengthening function of the intestinal probiotics to have pertinence and actual effect, and has visualization without complex analysis of results.
Drawings
FIG. 1 is a comparison under microscope between the intestinal tract of chickens bred with the additive and the intestinal tract of normally bred chickens not using the additive, which are prepared by the method for promoting the growth and development of intestinal structures of young animals.
FIG. 2 is a schematic diagram of abnormal egg rate in the fourth example of the preparation method of the additive for promoting intestinal structure growth and development of young animals.
Fig. 3 is a graph comparing eggs laid in the fourth example of the preparation method of the additive for promoting intestinal structure growth and development of young animals.
FIG. 4 is a diagram showing the Salmonella positive rate and the survival rate of chicks after the purification of parental generation in example four of the method for preparing the additive for promoting the growth and development of intestinal structures of young animals according to the present invention.
FIG. 5 is a schematic diagram of the molecular formula and molecular weight of seventeen cyclic dipeptides of the preparation method of the additive for promoting the intestinal structure growth and development of young animals.
Detailed Description
A preparation method of an additive for promoting the growth and development of intestinal structures of young animals comprises the following steps:
the method comprises the following steps: screening and culturing specific probiotic strains with functional peptides such as metabolic cyclic dipeptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003;
step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the functional peptides such as the metabolite cyclic dipeptide and the like, and liquid synbiotics of the probiotics and the metabolite are prepared;
step three: solid-state processing, separating, and purifying to obtain solid mixture of the above specific probiotics and metabolite cyclic dipeptide functional peptides, and making into synbiotics powder of probiotics and metabolite;
step four: and (2) screening the prebiotics which are favorable for the growth and the breeding of the specific probiotic strains and have good stability: polysaccharides, oligosaccharides, microalgae, flavonoids, and the like;
step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive.
The preparation process comprises the following steps:
(1) and strain: lactobacillus plantarum: lactic acid strain L-2003: 1010cfu/g。
(2) Preparing liquid: culturing and fermenting the culture solution.
(3) And liquid fermentation conditions: the culture solution is inactivated and fermented for 72 hours at the pH value of 4 and the temperature of 30 ℃.
(4) And preparing powder: and (5) drying and spraying in vacuum.
In conclusion, the mixed type probiotics rich in specific prebiotics, specific probiotics and specific probiotics is prepared by the processes of screening, culturing, fermenting, solidifying, purifying, mixing and the like, so that the mixed type probiotics have clear intestine-strengthening function and outstanding effect.
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, further experiments and data analysis will be performed on the additives produced by the present invention in combination with the embodiments of the present invention.
In the specific implementation of the invention, experiments are carried out in different chicken farms, and the following data are obtained through the experiments:
example one: the experimental site: six towns and large cai in plain county, tezhou; the experimental variety is as follows: roman 817; the number of the experimental sheds is as follows: 37000 the breeding is generally carried out for 55 days, 3.5 jin is taken out of the column, and the feed-meat ratio is 1.6-1.7; the additive products used in the present invention total: 43L.
Experimental data:
experimental day 8 weighing data:
experimental day 15 weighing data:
intestinal length measurement on experimental day 15:
the experimental conditions are as follows:
the analysis shows that 1, each chicken experimental group has more profit than the control group by 1.7 yuan, which is 1.2 times of the control group; 2. the feed-meat ratio is obviously reduced by nearly 2 percent; 3. weight average height analysis shows: (1) the feed-meat ratio of each chicken experimental group is reduced by nearly 2 percent (2), the average weight is 13.8 percent, and the growth speed is obviously accelerated; 4. the death and culling rate of the experimental group is 20 percent, and the reason why the death and culling rate of the experimental group is high in the early stage is that: the experimental group of the barns is earlier than the experimental group of the barns in construction, the early-stage constructed barns have bursal disease virus, and each group of the barns is required to have the bursal disease, so that the early-stage casualties of the experimental group are severe.
Example two: the experimental site: the Tongdai chicken farm in the development area of Binzhou city; the experimental variety is as follows: white feather broiler chicken; the number of the experimental sheds is as follows: 50000 pieces; 5 bottles of water are taken every day, and the water is concentrated and continuously used for 14 days; additive products of the invention were used together in total: 70L
Experimental data:
experimental day 8 weighing data:
experimental day 15 weighing data:
intestinal length measurement on experimental day 15:
intestinal length measurement on experimental day 15 (one longest and one shortest data mean, respectively):
example three: a chicken house 25500 feather and green-foot pheasant chick is used as an experimental group, 2L of the additive is added into each ton of water, and the chick is drunk for 14 days from 1 day old. The other 5 henhouses were fed with 13 million feathers as a control group as normal.
Data acquisition:
1. randomly selecting 20 live chickens from a control group and an experimental group on the 7 th day, the 14 th day and the experimental group respectively, weighing, repeating for 5 times (equivalent to the sample number of 100 chickens), and averaging the obtained weights to obtain the weight data of the group; meanwhile, the uniformity and the excrement condition of the chickens are observed and recorded.
2. On the 14 th day of the experiment, 4 live chickens are randomly selected from a control group and an experimental group respectively for dissection, the intestinal lengths of the live chickens are measured, and the average of the obtained data is used as the intestinal length data of the group; and observing the conditions of intestinal mucosa and the like.
3. The digital recording mode is adopted.
The experimental results are as follows:
1. weighing: the weight of the experimental group bodies on the 7 th day and the 14 th day is larger than that of the control group, and the weight is increased by nearly 30 percent.
2. And (4) intestinal tract dissection, wherein the intestinal tract length of the experimental group is obviously larger than that of the control group, and the intestinal mucosa is intact.
| Group of | Average length of small intestine | Morphology of small intestine |
| Control group | 91.25cm | Thin intestinal wall and more feed particles. |
| Experimental group | 107.75cm | Thick, thick intestine, intact mucous membrane, mostly minced. |
| Increase in growth | 16.5cm | |
| Rate of increase | 18.08％ |
Example four: breeding hens
The number of test chickens: 6580 hen houses No. 2 and 6050 hen houses No. 7 of parent white feather broiler breeders; week age of start test: 38 weeks; before the test: the positive rate of salmonella identified by parental breeding hen serotypes is 100%, and the death rate of offspring chicks is about 25%; and (3) test period: 2018.8.31-2018.9.9 (total 10 days); the test method comprises the following steps: adding the additive (powder) of the invention; and (3) test results: the purifying condition of the salmonella is analyzed by carrying out statistical analysis on abnormal eggs (malformed eggs, double-yellow eggs, broken eggs, preserved eggs and the like) of the laying hens.
The experimental results are as follows: no. 2 shed abnormal egg rate: 2.3% -4%; no. 7 shed abnormal egg rate: 2.6% -4%; after the parent-generation chick salmonella is purified, the positive rate of the salmonella is controlled within 10 percent, and the survival rate of the chick is improved by 15 percent.
Claims (1)
1. A preparation method of an additive for promoting the growth and development of intestinal structures of young animals is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: screening and culturing specific probiotic strains with the metabolic cyclic dipeptide functional peptide: lactobacillus plantarum L-2003, Lactobacillus sporogenes C-2003;
step two: liquid state fermentation is carried out to obtain a liquid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and liquid synbiotics of the probiotics and the metabolite are prepared;
step three: solid-state treatment, separation and purification to obtain a solid mixture of the specific probiotics and the metabolite cyclic dipeptide functional peptide, and preparing powder synbiotics of the probiotics and the metabolite;
step four: screening prebiotics which are beneficial to the growth and the breeding of the specific probiotic strains and have good stability;
step five: adding prebiotics into liquid synbiotics or powder synbiotics, and compounding to obtain the additive.
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| CN202011405318.XA CN114009584A (en) | 2020-12-03 | 2020-12-03 | Preparation method of additive for promoting growth and development of intestinal structures of young animals |
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| CN202011405318.XA CN114009584A (en) | 2020-12-03 | 2020-12-03 | Preparation method of additive for promoting growth and development of intestinal structures of young animals |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014049023A1 (en) * | 2012-09-25 | 2014-04-03 | Aquilon Cyl Sanidad Animal | Probiotic and prebiotic compositions |
| CN108835427A (en) * | 2018-08-15 | 2018-11-20 | 西北农林科技大学 | A kind of pregnant sow full price fermentative feedstuff of microbe preparation method |
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2020
- 2020-12-03 CN CN202011405318.XA patent/CN114009584A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014049023A1 (en) * | 2012-09-25 | 2014-04-03 | Aquilon Cyl Sanidad Animal | Probiotic and prebiotic compositions |
| CN108835427A (en) * | 2018-08-15 | 2018-11-20 | 西北农林科技大学 | A kind of pregnant sow full price fermentative feedstuff of microbe preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 屈思佳;李天密;高文惠;韩俊华;: "乳酸菌抗真菌活性及应用研究新进展", 中国乳品工业, no. 03, pages 39 - 42 * |
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