CN114008198B - 具有低副反应性的磷酸核酮糖3-差向异构酶基序和包含该基序的酶 - Google Patents

具有低副反应性的磷酸核酮糖3-差向异构酶基序和包含该基序的酶 Download PDF

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CN114008198B
CN114008198B CN202080028788.0A CN202080028788A CN114008198B CN 114008198 B CN114008198 B CN 114008198B CN 202080028788 A CN202080028788 A CN 202080028788A CN 114008198 B CN114008198 B CN 114008198B
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phosphate
epimerase
ribulose
psicose
amino acid
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CN114008198A (zh
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尹相咏
孙秉三
赵显国
朴炫俊
金承焕
梁成才
朴逸香
金成俌
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CJ CheilJedang Corp
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03001—Ribulose-phosphate 3-epimerase (5.1.3.1)
    • C—CHEMISTRY; METALLURGY
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN202080028788.0A 2019-05-21 2020-04-29 具有低副反应性的磷酸核酮糖3-差向异构酶基序和包含该基序的酶 Active CN114008198B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2019-0059697 2019-05-21
KR1020190059697A KR102080886B1 (ko) 2019-05-21 2019-05-21 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
PCT/KR2020/005701 WO2020235830A1 (ko) 2019-05-21 2020-04-29 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소

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CN114008198A CN114008198A (zh) 2022-02-01
CN114008198B true CN114008198B (zh) 2024-06-07

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US (1) US12371686B2 (https=)
EP (1) EP3916089A4 (https=)
JP (2) JP7410162B2 (https=)
KR (1) KR102080886B1 (https=)
CN (1) CN114008198B (https=)
AU (1) AU2020279663B2 (https=)
WO (1) WO2020235830A1 (https=)

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KR102080886B1 (ko) 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
US20220372535A1 (en) * 2019-10-28 2022-11-24 Samyang Corporation Fructose-6-phosphate 3-epimerase and use thereof
US20230183768A1 (en) * 2020-05-18 2023-06-15 Bonumose, Inc. Enzymatic production of allulose
CN120858175A (zh) * 2023-02-28 2025-10-28 嘉吉公司 用于生产d-阿洛酮糖的遗传修饰的微生物和发酵工艺

Citations (4)

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WO2003078643A1 (en) * 2002-03-19 2003-09-25 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of saccharomyces cerevisiae
CN106164265A (zh) * 2014-05-28 2016-11-23 大象（株） 阿洛酮糖差向异构酶和使用它生产阿洛酮糖的方法
WO2019027173A2 (ko) * 2017-07-31 2019-02-07 씨제이제일제당(주) 신규한 싸이코스-6-인산 탈인산효소, 상기 효소를 포함하는 사이코스 생산용 조성물, 상기 효소를 이용하여 사이코스를 제조하는 방법
CN109563499A (zh) * 2016-06-30 2019-04-02 Cj第制糖株式会社 一种新型热稳定果糖-6-磷酸盐-3-差向异构酶和使用其生产阿洛糖的方法

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WO2003084986A2 (en) * 2002-04-04 2003-10-16 Affinium Pharmaceuticals, Inc. Novel purified polypeptides involved in cellular metabolism
WO2004042043A2 (en) * 2002-11-05 2004-05-21 Affinium Pharmaceuticals, Inc. Crystal structures of bacterial ribulose-phosphate 3-epimerases
US10006033B2 (en) 2013-03-14 2018-06-26 The Regents Of The University Of California Recombinant microorganisms having a methanol elongation cycle (MEC)
KR102581107B1 (ko) * 2016-12-14 2023-09-21 보너모스, 인코포레이티드 D-알룰로스의 효소적 생산
WO2018129275A1 (en) 2017-01-06 2018-07-12 Greenlight Biosciences, Inc. Cell-free production of sugars
WO2018132871A1 (en) * 2017-01-17 2018-07-26 Macquarie University Method of producing chemicals in yeast
KR101965509B1 (ko) * 2017-11-15 2019-04-03 씨제이제일제당 (주) 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법
KR102080886B1 (ko) * 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소

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WO2003078643A1 (en) * 2002-03-19 2003-09-25 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of saccharomyces cerevisiae
CN106164265A (zh) * 2014-05-28 2016-11-23 大象（株） 阿洛酮糖差向异构酶和使用它生产阿洛酮糖的方法
CN109563499A (zh) * 2016-06-30 2019-04-02 Cj第制糖株式会社 一种新型热稳定果糖-6-磷酸盐-3-差向异构酶和使用其生产阿洛糖的方法
WO2019027173A2 (ko) * 2017-07-31 2019-02-07 씨제이제일제당(주) 신규한 싸이코스-6-인산 탈인산효소, 상기 효소를 포함하는 사이코스 생산용 조성물, 상기 효소를 이용하여 사이코스를 제조하는 방법

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CHAN et al.Structural Basis for Substrate Specificity in Phosphate Binding (β/α)8-Barrels:D-Allulose 6-Phosphate 3-Epimerase from Escherichia coli K-12.《Biochemistry》.2008,第第47卷卷第9608-9617页. *
D-阿洛酮糖3-差向异构酶的异源表达和酶学性质;温宇威;张涛;沐万孟;江波;;食品与生物技术学报;20180315(03);71-78 *
Structural Basis for Substrate Specificity in Phosphate Binding (β/α)8-Barrels:D-Allulose 6-Phosphate 3-Epimerase from Escherichia coli K-12;CHAN et al;《Biochemistry》;第第47卷卷;第9608-9617页 *

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Publication number Publication date
CN114008198A (zh) 2022-02-01
KR102080886B1 (ko) 2020-02-24
US12371686B2 (en) 2025-07-29
WO2020235830A1 (ko) 2020-11-26
US20230051519A1 (en) 2023-02-16
JP2022522693A (ja) 2022-04-20
JP7566993B2 (ja) 2024-10-15
JP7410162B2 (ja) 2024-01-09
AU2020279663B2 (en) 2023-04-13
EP3916089A4 (en) 2022-05-11
AU2020279663A1 (en) 2021-09-30
JP2023166380A (ja) 2023-11-21
EP3916089A1 (en) 2021-12-01

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