CN113980809A - Scientific experimental device for normal growth of cells in artificial environment - Google Patents

Scientific experimental device for normal growth of cells in artificial environment Download PDF

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Publication number
CN113980809A
CN113980809A CN202111557581.5A CN202111557581A CN113980809A CN 113980809 A CN113980809 A CN 113980809A CN 202111557581 A CN202111557581 A CN 202111557581A CN 113980809 A CN113980809 A CN 113980809A
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Prior art keywords
disinfection
box body
cells
sides
artificial environment
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Inventor
叶青松
王伦云
杜兰英
任芙蓉
普晓珊
王馨雨
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Kefuyuan Regenerative Medicine Hubei Co ltd
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Kefuyuan Regenerative Medicine Hubei Co ltd
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Publication of CN113980809A publication Critical patent/CN113980809A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B1/00Cleaning by methods involving the use of tools
    • B08B1/30Cleaning by methods involving the use of tools by movement of cleaning members over a surface
    • B08B1/32Cleaning by methods involving the use of tools by movement of cleaning members over a surface using rotary cleaning members
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00Combinations of bioreactors or fermenters with other apparatus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

The invention discloses a scientific experimental device for enabling cells to normally grow in an artificial environment, which comprises a box body, a sealing door, a sterilizing box, a first screw rod, a second screw rod and a cleaning plate, wherein the sealing door is hinged to one side of the box body through a hinge, and fixing clamping blocks are fixedly connected to two sides of the upper end inside the box body respectively. Thereby the cleaning of the surface of the storage platform can be realized.

Description

Scientific experimental device for normal growth of cells in artificial environment
Technical Field
The invention belongs to the technical field of cell tissue culture, and particularly relates to a scientific experimental device for enabling cells to normally grow in an artificial environment.
Background
The carbon dioxide incubator is a device for culturing cells/tissues in vitro by simulating and forming a growth environment similar to cells/tissues in organisms in an incubator body, wherein the incubator requires stable temperature (37 ℃), stable CO2 level (5%), constant pH value (pH value: 7.2-7.4) and high relative saturation humidity (95%), and the carbon dioxide incubator is an advanced instrument for culturing cells, tissues and bacteria and is key equipment necessary for developing immunology, oncology, genetics and biological engineering.
The following problems are now presented for most carbon dioxide incubators currently on the market:
1. most of carbon dioxide incubators on the market are easy to adhere cell tissue substances in the incubators in the use process, if the cell tissue substances are not cleaned in time, the cell tissue substances are solidified in the incubators, and the subsequent cleaning is troublesome;
2. most of carbon dioxide incubators on the market need to disinfect and sterilize the incubator before use to remove microorganisms in the incubator, but the existing disinfection mode cannot disinfect the inner wall of the incubator, so that the disinfection is not thorough, and the culture of cell tissue is influenced;
3. most carbon dioxide incubators on the market need monitor temperature, humidity and carbon dioxide concentration in the incubator in the use at present, but because the incubator internal volume is great, consequently in actual monitoring process, need monitor different positions in the incubator.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the existing defects and provide a scientific experimental device for enabling cells to normally grow in an artificial environment, so as to solve the problem that the cleaning in culture is troublesome in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a scientific experimental device for enabling cells to normally grow in an artificial environment comprises a box body, a sealing door, a disinfection box, a first screw rod, a second screw rod and a cleaning plate, wherein one side of the box body is hinged with the sealing door through a hinge, both sides of the upper end in the box body are fixedly connected with a fixed fixture block, the upper surface of the fixed fixture block is provided with the disinfection box, the surface of the disinfection box is provided with an injection port, the bottom in the box body is fixedly connected with a storage platform, both sides in the box body are provided with rotating discs, one side surface of each of the two rotating discs is sleeved with a rotating belt, one side of each of the rotating discs is in transmission connection with a driving motor, the other side surface of each of the rotating discs is rotatably connected with the first rotating rod, the other end of each rotating rod is rotatably connected with the second rotating rod, the lower end of each rotating rod is fixedly connected to the upper surface of the cleaning plate, both sides of the lower surface of the cleaning plate are fixedly connected with a connecting plate, the connecting plate rotates and is connected with clean running roller, clean running roller one end transmission is connected with driving motor, clean running roller quantity sets up to a plurality ofly, and is a plurality of inside clean running roller one end all runs through the connecting band, clean running roller surface is provided with protruding structure.
Preferably, the inside both sides of disinfect box all are provided with small-size pump machine, disinfect box lower surface both sides all are provided with the conveyer pipe, the conveyer pipe lower extreme sets up at a disinfection board upper surface, a disinfection board is inside to be hollow structure, disinfection board lower surface is provided with disinfection shower nozzle one, a disinfection shower nozzle quantity sets up to a plurality ofly.
Preferably, the inside bottom both sides of box all rotate and are connected with lead screw one, the transmission of a lead screw upper end is connected with driving motor, a lead screw surface threaded connection has the lift ring, lift ring one side fixedly connected with disinfection board two, two inside hollow structure that be of disinfection board, two one sides of disinfection board are provided with the connecting pipe, the connecting pipe other end sets up at a disinfection board side, two side surfaces of disinfection board are provided with disinfection shower nozzle two, disinfection shower nozzle two quantums set up to a plurality ofly.
Preferably, the equal fixedly connected with gag lever post in inside bottom both sides of box, gag lever post surface sliding connection has the spacing ring, spacing ring one side fixed connection is at the lifting ring side.
Preferably, both ends are the fixed plate of equal fixedly connected with about sealing door one side, the inside both sides of fixed plate are all rotated and are connected with lead screw two, two inside the drive track is all run through to two one ends of lead screw, inside being provided with driving motor of drive track.
Preferably, the two surfaces of the screw rod are in threaded connection with moving rings, and a moving plate is fixedly connected in the middle of the moving rings.
Preferably, both ends of one side of the moving plate are provided with ultraviolet lamps, the surface of the moving plate is provided with a humidity sensor, the upper end of the humidity sensor is provided with a temperature sensor, and the lower end of the humidity sensor is provided with a concentration sensor.
Preferably, the two sides of the lower end of the sealing door are both provided with carbon dioxide reactors, one side of the sealing door is provided with a heating plate, and the upper end of the heating plate is provided with a humidifier.
Preferably, the outer side surface of the sealing door is provided with a display.
Preferably, an alarm is arranged on one side of the upper surface of the box body.
Compared with the prior art, the invention provides a scientific experimental device for enabling cells to normally grow in an artificial environment, which has the following beneficial effects:
1. according to the invention, through the arranged rotating disc, the rotating disc can be driven to rotate by the driving motor, the first rotating rod on the surface of the rotating disc can be rotated, through the rotation of the first rotating rod, and through the matching between the first rotating rod and the second rotating rod, the position of the cleaning plate at the lower end of the second rotating rod can be moved up and down in a reciprocating manner, through the arranged cleaning rollers, the driving motor can drive the cleaning rollers to rotate through the connecting belt, and the cleaning rollers are driven by the cleaning plates to move up and down on the surface of the storage platform, so that the cell tissue remained on the surface of the storage platform can be cleaned for multiple times in a circulating manner, and the surface of the storage platform can be cleaned;
2. according to the invention, by the small pump, the disinfection solution in the disinfection box can be sprayed on the storage platform and the inner bottom end of the box body through the first disinfection nozzle on one hand, and the disinfection solution can be sprayed on the inner wall surface of the box body through the second disinfection nozzle on the other hand, so that different positions in the box body can be comprehensively disinfected, and the disinfection effect in the incubator is improved;
3. according to the invention, the first screw rod is arranged, the first screw rod can be driven by the driving motor to rotate, the position of the lifting ring on the surface of the first screw rod can be moved, and the height position of the second disinfection plate can be adjusted by moving the position of the lifting ring, so that the height position of the second disinfection spray head can be adjusted, the whole inner wall of the box body can be disinfected, and the limiting rod and the limiting ring are arranged, so that the limiting ring can be driven to slide on the limiting rod in the process of moving the surface of the first screw rod by the lifting ring, and the lifting ring can be prevented from rotating;
4. according to the invention, through the arranged driving crawler, the driving crawler can be driven to rotate by the driving motor in the driving crawler, the two screw rods II can be enabled to rotate together, and the moving ring positions on the surfaces of the screw rods II can be enabled through the rotation of the screw rods II, so that the position of the moving plate in the middle of the moving ring can be adjusted, the height positions of the humidity sensor, the temperature sensor and the concentration sensor on one side of the moving plate can be adjusted, and the humidity, the temperature and the carbon dioxide concentration at different positions in the box body can be monitored;
5. according to the invention, the humidity, the temperature and the carbon dioxide concentration in the box body can be respectively monitored by the humidity sensor, the temperature sensor and the concentration sensor, when the numerical value is too low or too high, an alarm can be given by the alarm to realize real-time monitoring of the humidity, the temperature and the carbon dioxide concentration in the box body, the disinfection effect in the box body can be further increased by the arranged ultraviolet lamp, and the humidity, the carbon dioxide concentration and the temperature in the box body can be respectively adjusted by the arranged humidifier, the carbon dioxide reactor and the heating plate.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention without limiting the invention in which:
FIG. 1 is a schematic structural diagram of a scientific experimental apparatus for normal growth of cells in an artificial environment according to the present invention;
FIG. 2 is a cross-sectional view of the scientific experimental apparatus for growing cells normally in artificial environment according to the present invention;
FIG. 3 is a rear view of a sealing door in a scientific experimental apparatus for normal growth of cells in an artificial environment according to the present invention;
FIG. 4 is a bottom view of a cleaning plate in a scientific experimental apparatus for normal growth of cells in an artificial environment according to the present invention;
FIG. 5 is an enlarged view of A in FIG. 2 of the scientific experimental apparatus for growing cells normally in artificial environment according to the present invention;
FIG. 6 is a front view of a movable plate in a scientific experimental apparatus for normal growth of cells in an artificial environment according to the present invention;
FIG. 7 is a rear view of a rotary disk in a scientific experimental apparatus for normal growth of cells in an artificial environment according to the present invention;
in the figure: 1. a box body; 2. a sealing door; 3. a display; 4. a sterilizing box; 5. a small pump; 6. a delivery pipe; 7. sterilizing a first plate; 8. a first disinfection spray nozzle; 9. a first screw rod; 10. a lifting ring; 11. a second disinfection plate; 12. a limiting rod; 13. a limiting ring; 14. rotating the disc; 15. rotating the first rod; 16. rotating the second rod; 17. cleaning the plate; 18. a connecting plate; 19. cleaning the roller; 20. a connecting belt; 21. a storage platform; 22. fixing the fixture block; 23. a fixing plate; 24. a second screw rod; 25. a moving ring; 26. a second disinfection spray nozzle; 27. a connecting pipe; 28. moving the plate; 29. a temperature sensor; 30. a humidity sensor; 31. a concentration sensor; 32. a carbon dioxide reactor; 33. an ultraviolet lamp; 34. a humidifier; 35. heating the plate; 36. driving the caterpillar; 37. an alarm; 38. the belt is rotated.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-7, the present invention provides the following technical solutions: a scientific experimental device for enabling cells to normally grow in an artificial environment comprises a box body 1, a sealing door 2, a disinfection box 4, a first screw rod 9, a second screw rod 24 and a cleaning plate 17, wherein the sealing door 2 is hinged on one side of the box body 1 through a hinge, fixing clamping blocks 22 are fixedly connected on two sides of the upper end in the box body 1, the disinfection box 4 is arranged on the upper surface of each fixing clamping block 22, an injection port is formed in the surface of the disinfection box 4, a storage platform 21 is fixedly connected at the bottom end in the box body 1, rotating discs 14 are arranged on two sides in the box body 1, rotating belts 38 are sleeved on one side surfaces of the two rotating discs 14, a driving motor is connected on one side of each rotating disc 14 in a transmission manner, a first rotating rod 15 is rotatably connected on the other side surface of each rotating disc 14, a second rotating rod 16 is rotatably connected on the other end of each rotating rod 15, the lower end of each rotating disc 16 is fixedly connected on the upper surface of the cleaning plate 17, and connecting plates 18 are fixedly connected on two sides of the lower surface of the cleaning plate 17, the connecting plate 18 is rotatably connected with a cleaning roller 19, one end of the cleaning roller 19 is in transmission connection with a driving motor, the number of the cleaning roller 19 is multiple, one end of each cleaning roller 19 penetrates through the inside of the connecting belt 20, a protruding structure is arranged on the surface of each cleaning roller 19, the driving motor drives the rotating disc 14 to rotate, the rotating rod one 15 on the surface of the rotating disc 14 can rotate, the rotating rod one 15 rotates, the cleaning plate 17 at the lower end of the rotating rod two 16 can reciprocate up and down through the matching between the rotating rod one 15 and the rotating rod two 16, the driving motor drives the cleaning rollers 19 to rotate through the connecting belt 20, the cleaning rollers 19 are driven by the cleaning plates 17 to move up and down on the surface of the storage platform 21, and cell tissue remaining on the surface of the storage platform 21 can be cleaned circularly for multiple times through the surface friction between the cleaning rollers 19 and the storage platform 21, thereby enabling cleaning of the surface of the storage platform 21.
In the invention, preferably, the small-sized pumps 5 are arranged on two sides inside the disinfection box 4, the conveying pipes 6 are arranged on two sides of the lower surface of the disinfection box 4, the lower end of each conveying pipe 6 is arranged on the upper surface of the first disinfection plate 7, the inside of the first disinfection plate 7 is of a hollow structure, the lower surface of the first disinfection plate 7 is provided with the first disinfection nozzles 8, the number of the first disinfection nozzles 8 is multiple, the disinfection agents in the disinfection box 4 can be pumped into the conveying pipes 6 through the operation of the small-sized pumps 5 in the disinfection box 4, and the disinfectants are sprayed from the first disinfection nozzles 8 on the lower surface of the first disinfection plate 7 to the bottom end of the box body 1 and the upper surface of the storage platform 21 through the conveying pipes 6, so that the bottom end inside the box body 1 and the upper surface of the storage platform 21 can be disinfected.
In the invention, preferably, both sides of the bottom end inside the box body 1 are rotatably connected with a first screw rod 9, the upper end of the first screw rod 9 is in transmission connection with a driving motor, a lifting ring 10 is in threaded connection with the surface of the first screw rod 9, a second disinfection plate 11 is fixedly connected with one side of the lifting ring 10, the interior of the second disinfection plate 11 is of a hollow structure, a connecting pipe 27 is arranged at one side of the second disinfection plate 11, the other end of the connecting pipe 27 is arranged at the side edge of the first disinfection plate 7, a second disinfection spray nozzle 26 is arranged on the surface of one side of the second disinfection plate 11, the number of the second disinfection spray nozzles 26 is multiple, the first screw rod 9 is driven by the driving motor to rotate, the position of the lifting ring 10 on the surface of the first screw rod 9 can be moved, the height position of the second disinfection plate 11 can be adjusted through the position movement of the lifting ring 10, so that the height position of the second disinfection spray nozzle 26 can be adjusted, and the whole inner wall of the box body 1 can be disinfected.
In the invention, preferably, both sides of the bottom end inside the box body 1 are fixedly connected with limiting rods 12, the surface of each limiting rod 12 is connected with a limiting ring 13 in a sliding manner, one side of each limiting ring 13 is fixedly connected to the side edge of each lifting ring 10, and the limiting rings 13 can be driven to slide on the limiting rods 12 in the surface movement process of the first lead screw 9 through the lifting rings 10, so that the lifting rings 10 can be prevented from rotating.
In the invention, preferably, the upper end and the lower end of one side of the sealing door 2 are fixedly connected with the fixing plate 23, two sides inside the fixing plate 23 are rotatably connected with the second screw rods 24, one ends of the two second screw rods 24 penetrate through the inside of the driving crawler 36, the driving motor is arranged inside the driving crawler 36, and the driving motor inside the driving crawler 36 drives the driving crawler 36 to rotate, so that the two second screw rods 24 can rotate together.
In the invention, preferably, the surface of the second screw 24 is in threaded connection with the moving ring 25, the moving plate 28 is fixedly connected in the middle of the moving ring 25, the position of the moving ring 25 on the surface of the second screw 24 can be moved by rotating the second screw 24, the position of the moving plate 28 in the middle of the moving ring 25 can be adjusted, the height positions of the humidity sensor 30, the temperature sensor 29 and the concentration sensor 31 on one side of the moving plate 28 can be adjusted, and the humidity, the temperature and the carbon dioxide concentration at different positions in the box body 1 can be monitored.
In the present invention, it is preferable that the ultraviolet lamps 33 are provided at both ends of one side of the moving plate 28, the humidity sensor 30 is provided on the surface of the moving plate 28, the temperature sensor 29 is provided at the upper end of the humidity sensor 30, the concentration sensor 31 is provided at the lower end of the humidity sensor 30, and the humidity, the temperature and the carbon dioxide concentration in the cabinet 1 can be monitored by operating the humidity sensor 30, the temperature sensor 29 and the concentration sensor 31.
In the invention, preferably, the two sides of the lower end of the sealing door 2 are both provided with the carbon dioxide reactor 32, one side of the sealing door 2 is provided with the heating plate 35, the upper end of the heating plate 35 is provided with the humidifier 34, and the humidifier 34, the carbon dioxide reactor 32 and the heating plate 35 operate to respectively adjust the humidity, the carbon dioxide concentration and the temperature in the box body 1, so that the environment in the box body 1 reaches a proper condition.
In the present invention, it is preferable that the display 3 is provided on the outer surface of the sealing door 2 to display the temperature, humidity and carbon dioxide concentration inside the cabinet 1.
In the invention, preferably, an alarm 37 is arranged on one side of the upper surface of the box body 1, and when the humidity, the carbon dioxide concentration and the temperature value in the box body 1 are too low or too high, the alarm 37 can give an alarm to realize real-time monitoring of the humidity, the temperature and the carbon dioxide concentration in the box body 1.
The working principle and the using process of the invention are as follows: when in use, the interior of the box body 1 is disinfected firstly, the small pump 5 is operated, on one hand, disinfectant in the disinfection box 4 can be sprayed on the bottom ends of the storage platform 21 and the interior of the box body 1 through the disinfection spray head I8, on the other hand, the disinfectant can be sprayed on the surface of the inner wall of the box body 1 through the disinfection spray head II 26, on the other hand, comprehensive disinfection on different positions in the box body 1 can be realized through the mode, the disinfection effect in the incubator is improved, the screw rod I9 is driven to rotate through the driving motor, the position of the lifting ring 10 on the surface of the screw rod I9 can be moved, the height position of the disinfection plate II 11 can be adjusted through the position movement of the lifting ring 10, so that the height position of the disinfection spray head II 26 can be adjusted, the whole inner wall of the box body 1 can be disinfected, the limiting ring 13 can be driven to slide on the limiting rod 12 through the lifting ring 10 in the surface movement process of the screw rod I9, can avoid the lifting ring 10 to appear the condition of rotation, after the disinfection, place the cell tissue thing that needs to cultivate on depositing the platform 21 surface, close sealing door 2, through humidifier 34, carbon dioxide reactor 32 and the operation of heating board 35, can respectively to box 1 internal humidity, carbon dioxide concentration and temperature adjust, thereby make box 1 internal environment reach suitable condition, and through humidity transducer 30, temperature sensor 29 and concentration sensor 31 operation, can respectively to box 1 internal humidity, temperature and carbon dioxide concentration monitor, when the value appears crossing excessively low or too high condition, can send out the police dispatch newspaper through siren 37, realize carrying out real time monitoring to box 1 internal humidity, temperature and carbon dioxide concentration, drive track 36 through drive track 36 internal drive motor and rotate, can make two lead screws two 24 rotate together, the position of the moving ring 25 on the surface of the second screw rod 24 can be moved through the rotation of the second screw rod 24, the position of the moving plate 28 in the middle of the moving ring 25 can be adjusted, so that the height positions of the humidity sensor 30, the temperature sensor 29 and the concentration sensor 31 on one side of the moving plate 28 can be adjusted, the humidity, the temperature and the carbon dioxide concentration at different positions in the box body 1 can be monitored, when cell tissues are taken out, the rotating disc 14 is driven to rotate through the driving motor, the rotating rod one 15 on the surface of the rotating disc 14 can be rotated, the cleaning plate 17 at the lower end of the rotating rod two 16 can be moved up and down in a reciprocating mode through the matching between the rotating rod one 15 and the rotating rod two 16, the plurality of cleaning rollers 19 are driven to rotate through the connecting belt 20 through the driving motor, and the cleaning rollers 19 are driven to move up and down on the surface of the storage platform 21 through the cleaning plate 17, through cleaning roller 19 and deposit platform 21 skin friction, can realize depositing remaining cell tissue thing in platform 21 surface and carry out the clearance of circulation many times to can realize depositing the clearance on platform 21 surface.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The utility model provides a make scientific experiment device of cell normal growth in artificial environment, includes box (1), sealing door (2), disinfect box (4), lead screw one (9), lead screw two (24) and cleaning plate (17), its characterized in that: the automatic cleaning device is characterized in that a sealing door (2) is hinged to one side of the box body (1) through a hinge, fixed clamping blocks (22) are fixedly connected to two sides of the upper end inside the box body (1), a sterilizing box (4) is arranged on the upper surface of each fixed clamping block (22), an injection port is formed in the surface of each sterilizing box (4), a storage platform (21) is fixedly connected to the bottom end inside the box body (1), rotating discs (14) are arranged on two sides inside the box body (1), a rotating belt (38) is sleeved on the surface of one side of each rotating disc (14), a driving motor is connected to one side of each rotating disc (14), a rotating rod I (15) is rotatably connected to the surface of the other side of each rotating disc (14), a rotating rod II (16) is rotatably connected to the other end of each rotating rod I (15), the lower end of each rotating rod II (16) is fixedly connected to the upper surface of a cleaning plate (17), and connecting plates (18) are fixedly connected to two sides of the lower surface of the cleaning plate (17), connecting plate (18) rotate and are connected with clean running roller (19), clean running roller (19) one end transmission is connected with driving motor, clean running roller (19) quantity sets up to a plurality ofly, and is a plurality of inside clean running roller (19) one end all runs through connecting band (20), clean running roller (19) surface is provided with protruding structure.
2. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: all be provided with minipump machine (5) in disinfect box (4) inside both sides, disinfect box (4) lower surface both sides all are provided with conveyer pipe (6), conveyer pipe (6) lower extreme sets up at disinfection board (7) upper surface, disinfection board (7) are inside to be hollow structure, disinfection board (7) lower surface is provided with disinfection shower nozzle (8), disinfection shower nozzle (8) quantity sets up to a plurality ofly.
3. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: the utility model discloses a disinfection cabinet, including box (1), inside bottom both sides all rotate and are connected with lead screw (9), the transmission of lead screw (9) upper end is connected with driving motor, lead screw (9) surface threaded connection has lift ring (10), lift ring (10) one side fixedly connected with disinfection board two (11), disinfection board two (11) are inside to be hollow structure, disinfection board two (11) one side is provided with connecting pipe (27), connecting pipe (27) other end setting is at disinfection board one (7) side, disinfection board two (11) a side surface is provided with disinfection shower nozzle two (26), disinfection shower nozzle two (26) quantity sets up to a plurality ofly.
4. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: the box body (1) is characterized in that limiting rods (12) are fixedly connected to two sides of the inner bottom end of the box body (1), limiting rings (13) are connected to the surfaces of the limiting rods (12) in a sliding mode, and one sides of the limiting rings (13) are fixedly connected to the side edges of the lifting rings (10).
5. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: the sealing door is characterized in that a fixing plate (23) is fixedly connected to the upper end and the lower end of one side of the sealing door (2), a second screw rod (24) is rotatably connected to the two sides of the inside of the fixing plate (23), one end of the second screw rod (24) penetrates through the inside of the driving crawler (36), and a driving motor is arranged inside the driving crawler (36).
6. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: the surface of the second screw rod (24) is in threaded connection with a moving ring (25), and a moving plate (28) is fixedly connected in the middle of the moving ring (25).
7. A scientific experimental facility for enabling cells to grow normally in artificial environment according to claim 6, characterized in that: both ends of one side of the moving plate (28) are provided with ultraviolet lamps (33), the surface of the moving plate (28) is provided with a humidity sensor (30), the upper end of the humidity sensor (30) is provided with a temperature sensor (29), and the lower end of the humidity sensor (30) is provided with a concentration sensor (31).
8. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: the carbon dioxide reactor (32) is arranged on two sides of the lower end of the sealing door (2), the heating plate (35) is arranged on one side of the sealing door (2), and the humidifier (34) is arranged at the upper end of the heating plate (35).
9. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: and a display (3) is arranged on the outer side surface of the sealing door (2).
10. A scientific experimental facility for enabling normal growth of cells in artificial environment according to claim 1, characterized in that: and an alarm (37) is arranged on one side of the upper surface of the box body (1).
CN202111557581.5A 2021-03-22 2021-12-19 Scientific experimental device for normal growth of cells in artificial environment Pending CN113980809A (en)

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