CN208857308U - Stem cell culture perfusing device - Google Patents
Stem cell culture perfusing device Download PDFInfo
- Publication number
- CN208857308U CN208857308U CN201821281152.3U CN201821281152U CN208857308U CN 208857308 U CN208857308 U CN 208857308U CN 201821281152 U CN201821281152 U CN 201821281152U CN 208857308 U CN208857308 U CN 208857308U
- Authority
- CN
- China
- Prior art keywords
- stem cell
- wall
- cell culture
- perfusion bottle
- electric telescopic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses stem cell culture perfusing devices, including perfusion bottle, the two sides of the perfusion bottle outer circumferential walls are plugged with delivery pipe, and two delivery pipes have discharge pipe by flanged joint far from one end of perfusion bottle, there is solenoid valve in the bottom of the discharge pipe by flanged joint, and the two sides of perfusion top of bottle outer wall are equipped with mounting shell, the middle position of two mounting shell top inner walls is equipped with the first electric telescopic rod, and two the first electric telescopic rods are equipped with the same outlet pipe far from one end of mounting shell, the spray head equidistantly distributed is arranged by flanged joint at the bottom of the outlet pipe outer circumferential walls, and the top of outlet pipe outer circumferential walls is equipped with scraper plate.The utility model realizes flushing and scraping to perfusion bottle circumferential inner wall, convenient for the cleaning to perfusion bottle circumferential inner wall, it is effectively prevent stem cell drippage extra at discharge pipe, guarantees uniformly sprinkling culture solution, and culture-liquid temp is suitable for, and improves the success rate of stem cell culture.
Description
Technical field
The utility model relates to stem cells technology field more particularly to stem cell culture perfusing devices.
Background technique
Stem cell is a kind of multipotential cell with the of self-replication capacity, and under certain condition, it can be divided into more
Kind functioning cell, the stage of development according to locating for stem cell is divided into embryonic stem cell and adult stem cell, according to the hair of stem cell
Potential is educated to be divided into three classes；Myeloid-lymphoid stem cell, multipotential stem cell and unipotent stem cell, stem cell are a kind of inabundant differentiation, still not
Mature cell has the potential function for regenerating various histoorgans and human body, and medical field is known as " general-purpose cell ", dry at present thin
The perfusion device of born of the same parents' culture is generally made of several culture dishes and flask, there are culture solution sprinklings when use uneven, perfusion bottle
The problem of inner wall washing inconvenience.
Summary of the invention
Purpose of the utility model is to solve disadvantages existing in the prior art, and the stem cell proposed cultivates perfusion
Device.
To achieve the goals above, the utility model adopts the technical scheme that
The two sides of stem cell culture perfusing device, including perfusion bottle, the perfusion bottle outer circumferential walls are plugged with delivery pipe,
And there are discharge pipe in two one end of delivery pipe far from perfusion bottle by flanged joint, the bottom of the discharge pipe passes through flanged joint
There is solenoid valve, and the two sides of perfusion top of bottle outer wall are equipped with mounting shell, the middle position of two mounting shell top inner walls
It is equipped with the first electric telescopic rod, and two the first electric telescopic rods are equipped with the same outlet pipe, institute far from one end of mounting shell
The spray head equidistantly distributed is arranged by flanged joint at the bottom for stating outlet pipe outer circumferential walls, and sets at the top of outlet pipe outer circumferential walls
There is scraper plate, the middle position of the perfusion top of bottle inner wall is equipped with coupling tube, and the bottom outer wall of coupling tube passes through flanged joint
There is atomizer, the middle position of the perfusion top of bottle outer wall is equipped with cabinet, and the bottom interior wall of cabinet is equipped with adding thermal resistance
Silk, the middle position of the top of the box outer wall is equipped with stirring motor, and the bottom of cabinet side outer wall is equipped with delivery pump, described
The side of perfusion bottle bottom outer wall is set there are two extension rod, and the bottom of two extension rod sides is equipped with the same mounting plate.
Preferably, there are connecting tube, and the one end of connecting tube far from delivery pump in the side of the delivery pump by flanged joint
Extend to the inside of coupling tube.
Preferably, the bottom of two extension rod sides is equipped with the second electric telescopic rod, and two second electronic are stretched
Contracting bar is equipped with the same catch tray far from one end of extension rod.
Preferably, the middle position of the collector tray bottom outer wall offers sliding slot, and the side at the top of mounting plate is equipped with
Pulley.
Preferably, the outer diameter of the pulley and the internal diameter of sliding slot are adapted, and catch tray is located at the bottom of discharge pipe.
Preferably, the output shaft of the stirring motor is equipped with stirring rod, and the bottom of stirring rod circumference is equipped with equidistant point
The agitating paddle of cloth.
Preferably, the solenoid valve, the first electric telescopic rod, delivery pump, stirring motor, the second electric telescopic rod and heating
Resistance wire passes through conducting wire and is connected with switch, and switchs and be connected with power supply by conducting wire.
The utility model has the following beneficial effects:
1. be provided with outlet pipe, spray head, the first electric telescopic rod and scraper plate, by outlet pipe and spray head to the circle of perfusion bottle
All inner walls are rinsed, while the first electric telescopic rod pushes outlet pipe to move downward, and scraper plate scrapes the circumferential inner wall of perfusion bottle
Only, flushing and the scraping to perfusion bottle circumferential inner wall are realized, convenient for the cleaning to perfusion bottle circumferential inner wall.
2. being provided with the second electric telescopic rod and catch tray, when the discharge of stem cell reaches discharge rate, solenoid valve is closed, together
When the second electric telescopic rod push catch tray to be moved to the bottom of discharge pipe, effectively prevent stem cell drop extra at discharge pipe
It falls.
3. being provided with resistive heater, stirring motor and atomizer, resistive heater is powered to the intracorporal culture solution of case
It is heated, stirring motor drives agitating paddle to be stirred culture solution, and delivery pump extracts the intracorporal culture solution of case and passes through atomization
Spray head guarantees uniformly sprinkling culture solution to uniformly being sprayed in perfusion bottle, and culture-liquid temp is suitable for, and improves stem cell culture
Success rate.
Detailed description of the invention
Fig. 1 be the utility model proposes stem cell culture perfusing device structural schematic diagram；
Fig. 2 be the utility model proposes stem cell culture perfusing device mounting plate side structure cross-sectional view；
Fig. 3 be the utility model proposes stem cell culture perfusing device perfusion bottle local side structure sectional view.
In figure: 1 perfusion bottle, 2 delivery pipes, 3 extension rods, 4 catch trays, 5 mounting plates, 6 solenoid valves, 7 discharge pipes, 8 spray heads, 9
Scraper plate, 10 first electric telescopic rods, 11 mounting shells, 12 connecting tubes, 13 delivery pumps, 14 cabinets, 15 stirring motors, 16 outlet pipes,
17 second electric telescopic rods, 18 sliding slots, 19 pulleys, 20 resistive heaters, 21 coupling tubes, 22 atomizers.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.
Referring to Fig.1-3, stem cell culture perfusing device, including perfusion bottle 1, the equal grafting in two sides of 1 outer circumferential walls of perfusion bottle
There is delivery pipe 2, and there are discharge pipe 7 in two one end of delivery pipe 2 far from perfusion bottle 1 by flanged joint, the bottom of discharge pipe 7 is logical
Crossing flanged joint has a solenoid valve 6, and the two sides of 1 top exterior walls of perfusion bottle pass through fastening bolt and are connected with mounting shell 11, and two
The middle position of 11 top inner wall of mounting shell passes through fastening bolt and is connected with the first electric telescopic rod 10, and two first electronic
The one end of telescopic rod 10 far from mounting shell 11 is connected with the same outlet pipe 16 by fastening bolt, 16 top exterior walls of outlet pipe
Side is plugged with water inlet pipe, and the spray head 8 equidistantly distributed is arranged by flanged joint at the bottom of 16 outer circumferential walls of outlet pipe, and is discharged
The top of 16 outer circumferential walls of pipe is connected with scraper plate 9, side of the scraper plate 9 far from outlet pipe 16 and perfusion bottle 1 by fastening bolt
Circumferential inner wall contact, the middle position of 1 top inner wall of perfusion bottle are connected with coupling tube 21 by fastening bolt, and coupling tube 21
Bottom outer wall has atomizer 22 by flanged joint, and the middle position of 1 top exterior walls of perfusion bottle is connected with by fastening bolt
Cabinet 14, and the bottom interior wall of cabinet 14 is connected with resistive heater 20, the centre of 14 top exterior walls of cabinet by fastening bolt
Position is connected with stirring motor 15 by fastening bolt, and the bottom of 14 side outer wall of cabinet is connected with conveying by fastening bolt
Pump 13, delivery pump 13 are connected by fastening bolt with the top exterior walls of perfusion bottle 1, and the side of 1 bottom outer wall of perfusion bottle passes through tight
There are two extension rods 3 for fixing bolt connection, and the bottom of two 3 sides of extension rod is connected with the same mounting plate by fastening bolt
5。
In the utility model, there is connecting tube 12 in the side of delivery pump 13 by flanged joint, and connecting tube 12 is far from conveying
One end of pump 13 extends to the inside of coupling tube 21, and the bottom of two 3 sides of extension rod passes through fastening bolt and is connected with second
Electric telescopic rod 17, and two second one end of electric telescopic rod 17 far from extension rod 3 be connected with by fastening bolt it is same
Catch tray 4, the middle position of 4 bottom outer wall of catch tray offers sliding slot 18, and the side at 5 top of mounting plate passes through fastening bolt
It is connected with pulley 19, the outer diameter of pulley 19 and the internal diameter of sliding slot 18 are adapted, and catch tray 4 is located at the bottom of discharge pipe 7, stirring
The output shaft of motor 15 is connected with stirring rod by fastening bolt, and the bottom of stirring rod circumference is connected with by fastening bolt
The agitating paddle of range distribution, solenoid valve 6, the first electric telescopic rod 10, delivery pump 13, stirring motor 15, the second electric telescopic rod
17 and resistive heater 20 switch is connected with by conducting wire, and switch and power supply be connected with by conducting wire.
Working principle: the culture solution in cabinet 14 is heated in the energization of resistive heater 20, and the drive of stirring motor 15 is stirred
Paddle rotation is mixed, culture solution is stirred, when needing to add culture solution into perfusion bottle 1, delivery pump 13 is extracted in cabinet 14
Culture solution, to uniformly being sprayed in perfusion bottle 1, is incited somebody to action by coupling tube 21 and atomizer 22 by delivery pipe 2 and discharge pipe 7
Stem cell discharge in perfusion bottle 1, when reaching discharge rate, solenoid valve is closed, while the second electric telescopic rod 17 pushes catch tray 4
It is moved to the bottom of discharge pipe 7, during which pulley 19 slides in sliding slot 18, when the inner wall to perfusion bottle 1 being needed to clean, leads to
It crosses outlet pipe 16 and spray head 8 is rinsed the circumferential inner wall of perfusion bottle 1, while the first electric telescopic rod 10 pushes outlet pipe 16
It moves downward, scraper plate 9 shaves the circumferential inner wall of perfusion bottle 1.
The preferable specific embodiment of the above, only the utility model, but the protection scope of the utility model is not
It is confined to this, anyone skilled in the art is within the technical scope disclosed by the utility model, practical according to this
Novel technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model
Within enclosing.
Claims (7)
1. stem cell culture perfusing device, including perfusion bottle (1), which is characterized in that the two sides of perfusion bottle (1) outer circumferential walls
It is plugged with delivery pipe (2), and two delivery pipes (2) have discharge pipe (7) by flanged joint far from the one end of perfusion bottle (1),
The bottom of the discharge pipe (7) has solenoid valve (6) by flanged joint, and the two sides of perfusion bottle (1) top exterior walls are equipped with peace
It fills shell (11), the middle position of two mounting shell (11) top inner walls is equipped with the first electric telescopic rod (10), and two
First electric telescopic rod (10) is equipped with the same outlet pipe (16) far from the one end of mounting shell (11), outlet pipe (16) circumference
The spray head (8) equidistantly distributed is arranged by flanged joint at the bottom of outer wall, and the top of outlet pipe (16) outer circumferential walls is equipped with and scrapes
The middle position of plate (9), perfusion bottle (1) top inner wall is equipped with coupling tube (21), and the bottom outer wall of coupling tube (21) is logical
Crossing flanged joint has atomizer (22), and the middle position of perfusion bottle (1) top exterior walls is equipped with cabinet (14), and cabinet
(14) bottom interior wall is equipped with resistive heater (20), and the middle position of cabinet (14) top exterior walls is equipped with stirring motor
(15), and the bottom of cabinet (14) side outer wall is equipped with delivery pump (13), and the side of perfusion bottle (1) bottom outer wall is equipped with two
A extension rod (3), and the bottom of two extension rod (3) sides is equipped with the same mounting plate (5).
2. stem cell culture perfusing device according to claim 1, which is characterized in that the side of the delivery pump (13) is logical
Crossing flanged joint has connecting tube (12), and connecting tube (12) extends to the interior of coupling tube (21) far from the one end of delivery pump (13)
Portion.
3. stem cell culture perfusing device according to claim 1, which is characterized in that two extension rod (3) sides
Bottom be equipped with the second electric telescopic rod (17), and two the second electric telescopic rods (17) set far from the one end of extension rod (3)
There are the same catch tray (4).
4. stem cell culture perfusing device according to claim 3, which is characterized in that catch tray (4) bottom outer wall
Middle position offer sliding slot (18), and the side at the top of mounting plate (5) be equipped with pulley (19).
5. stem cell culture perfusing device according to claim 4, which is characterized in that the outer diameter and cunning of the pulley (19)
The internal diameter of slot (18) is adapted, and catch tray (4) is located at the bottom of discharge pipe (7).
6. stem cell culture perfusing device according to claim 1, which is characterized in that the output of the stirring motor (15)
Axis is equipped with stirring rod, and the bottom of stirring rod circumference is equipped with the agitating paddle equidistantly distributed.
7. stem cell culture perfusing device according to claim 1, which is characterized in that the solenoid valve (6), first electronic
Telescopic rod (10), delivery pump (13), stirring motor (15), the second electric telescopic rod (17) and resistive heater (20) are by leading
Line is connected with switch, and switchs and be connected with power supply by conducting wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821281152.3U CN208857308U (en) | 2018-08-09 | 2018-08-09 | Stem cell culture perfusing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821281152.3U CN208857308U (en) | 2018-08-09 | 2018-08-09 | Stem cell culture perfusing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208857308U true CN208857308U (en) | 2019-05-14 |
Family
ID=66414393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821281152.3U Expired - Fee Related CN208857308U (en) | 2018-08-09 | 2018-08-09 | Stem cell culture perfusing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208857308U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778162A (en) * | 2020-07-29 | 2020-10-16 | 湖北明德健康科技有限公司 | Stem cell culture perfusion device |
| CN111826287A (en) * | 2020-08-04 | 2020-10-27 | 湖北明德健康科技有限公司 | Improved cell culture perfusion device |
| CN113980809A (en) * | 2021-03-22 | 2022-01-28 | 科复源再生医学(湖北)有限公司 | Scientific experimental device for normal growth of cells in artificial environment |
-
2018
- 2018-08-09 CN CN201821281152.3U patent/CN208857308U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778162A (en) * | 2020-07-29 | 2020-10-16 | 湖北明德健康科技有限公司 | Stem cell culture perfusion device |
| CN111826287A (en) * | 2020-08-04 | 2020-10-27 | 湖北明德健康科技有限公司 | Improved cell culture perfusion device |
| CN113980809A (en) * | 2021-03-22 | 2022-01-28 | 科复源再生医学(湖北)有限公司 | Scientific experimental device for normal growth of cells in artificial environment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208857308U (en) | Stem cell culture perfusing device | |
| CN208590397U (en) | A kind of stainless steel double-sided slot convenient for cleaning | |
| CN202843552U (en) | Intelligent dish-washing machine | |
| CN107670063A (en) | A kind of medicine equipment cleaning and sterilizing processing unit | |
| CN109433690A (en) | A kind of high-efficient cleaning device for medical appliance | |
| CN206761977U (en) | A kind of surgery flusher capable of washing | |
| CN208960548U (en) | A kind of Plant Tissue Breeding ware chlorination equipment | |
| CN108245113A (en) | A kind of Intelligent dish washing machine with classification spin-cleaning tableware | |
| CN208720697U (en) | A kind of drying kettle of pharmaceutical intermediate | |
| CN208042739U (en) | A kind of Ultrasonic-B probe cleaning sterilizing device | |
| CN207914234U (en) | A kind of medical instrument cycle perfusion cleaning device | |
| CN207640127U (en) | Cooking apparatus | |
| CN205275958U (en) | Pressure boost cleaning equipment that adjusts temperature | |
| CN108968657A (en) | A kind of novel meal braizing machine | |
| CN108729172A (en) | Clothing drying device | |
| CN206285528U (en) | A kind of extractor | |
| CN205431991U (en) | Fruit type cleaning machine | |
| CN207341928U (en) | A kind of Chinese herbal medicine multi-function extractor | |
| CN207487495U (en) | A kind of central air-conditioning fan coiler cleaning apparatus | |
| CN208613185U (en) | A kind of field beast medical appliance cleaning device | |
| CN208551328U (en) | Cooking apparatus | |
| CN209463947U (en) | A kind of water-saving dish washer | |
| CN209577659U (en) | Put a chimney on the stove and make the fire draw can washing apparatus | |
| CN209108252U (en) | A kind of anti-hydrops formula wound irrigation syringe of vascular surgery | |
| CN208551526U (en) | Material cleaning device and cooking apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190514 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |