CN211272512U - A sterilization device for sterile culture medium - Google Patents
A sterilization device for sterile culture medium Download PDFInfo
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- CN211272512U CN211272512U CN201922210259.XU CN201922210259U CN211272512U CN 211272512 U CN211272512 U CN 211272512U CN 201922210259 U CN201922210259 U CN 201922210259U CN 211272512 U CN211272512 U CN 211272512U
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 49
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 48
- 239000001963 growth medium Substances 0.000 title claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 241000894006 Bacteria Species 0.000 claims description 5
- 210000005069 ears Anatomy 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 5
- 238000009434 installation Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
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Abstract
Description
技术领域technical field
本实用新型涉及无菌培养基领域,尤其涉及一种无菌培养基的灭菌装置。The utility model relates to the field of aseptic culture medium, in particular to a sterilization device for aseptic culture medium.
背景技术Background technique
培养基是指供微生物、植物组织和动物组织生长和维持用的人工配制的养料,一般都含有碳水化合物、含氮物质、无机盐以及维生素和水等,有的培养基还含有抗菌素、色素、激素和血清,不同培养基可根据实际需要,添加一些自身无法合成的化合物,即生长因子。由于培养基配制的原料不同,使用要求不同,而贮存保管方面也稍有不同。一般培养基在受热、吸潮后,易被细菌污染或分解变质,因此一般培养基必须防潮、避光、阴凉处保存。对一些需严格灭菌的培养基(如组织培养基),较长时间的贮存,必须放在2~6℃的冰箱内。Culture medium refers to the artificially prepared nutrients for the growth and maintenance of microorganisms, plant tissues and animal tissues, generally containing carbohydrates, nitrogenous substances, inorganic salts, vitamins and water, etc. Hormones and serum, different media can add some compounds that cannot be synthesized by themselves, that is, growth factors according to actual needs. Due to the different raw materials for the preparation of the medium, the requirements for use are different, and the storage and storage are also slightly different. Generally, the medium is easily contaminated or decomposed by bacteria after being heated and absorbing moisture. Therefore, the general medium must be kept in a cool and moisture-proof place. For some media that need to be strictly sterilized (such as tissue culture medium), they must be stored in a refrigerator at 2~6°C for a long time.
目前对于无菌培养基,都是采用先灭菌处理再转移至储存装置进行保存,但是现有的培养基灭菌装置每一次灭菌的量都比较大,对灭菌后的培养基从灭菌箱内拿取不方便,不能很好的应用于生产研究过程。At present, the sterile medium is sterilized first and then transferred to a storage device for storage. However, the amount of each sterilization of the existing medium sterilization device is relatively large, and the sterilized medium is never sterilized. The bacteria box is inconvenient to take and cannot be well applied to the production and research process.
实用新型内容Utility model content
本实用新型的目的是为了解决现有技术中存在的缺点,而提出的一种无菌培养基的灭菌装置。The purpose of the utility model is to propose a sterilization device for aseptic culture medium in order to solve the shortcomings existing in the prior art.
为了实现上述目的,本实用新型采用了如下技术方案:In order to achieve the above-mentioned purpose, the utility model adopts the following technical solutions:
一种无菌培养基的灭菌装置,包括操作箱,所述操作箱的内腔内壁中心处通过轴承转动连接有转轴,所述转轴的表面套接有齿轮,所述转轴的一端贯穿操作箱并延伸至操作箱的外部,所述转轴的延伸端连接有电机,所述齿轮的顶端啮合有齿条,所述齿条顶部的两侧分别固定连接有第一移动杆和第二移动杆,所述第一移动杆、第二移动杆的顶端分别固定有第一滑块、第二滑块,所述操作箱内腔的顶部开设有与第一滑块、第二滑块配合使用的第一滑槽、第二滑槽,所述第一滑块、第二滑块与第一滑槽、第二滑槽的内壁滑动连接,所述操作箱的顶部开设有位于第二滑槽上方的槽口,所述第二滑块的顶端固定有支撑杆;A sterilization device for sterile culture medium, comprising an operation box, a rotating shaft is rotatably connected to the center of the inner wall of the inner cavity of the operation box through a bearing, a gear is sleeved on the surface of the rotating shaft, and one end of the rotating shaft penetrates the operation box and extends to the outside of the operation box, the extension end of the rotating shaft is connected with a motor, the top of the gear is engaged with a rack, and the two sides of the top of the rack are fixedly connected with a first moving rod and a second moving rod, respectively, The top ends of the first moving rod and the second moving rod are respectively fixed with a first sliding block and a second sliding block, and the top of the inner cavity of the operation box is provided with a first sliding block and a second sliding block used in cooperation with the first sliding block and the second sliding block. A chute and a second chute, the first slider and the second slider are slidably connected with the inner walls of the first and second chute, and the top of the operation box is provided with a slider located above the second chute a notch, a support rod is fixed on the top end of the second sliding block;
操作箱的上表面左侧固定有灭菌箱,所述支撑杆的顶端固定有移动板,所述移动板上表面放置有放置板,所述放置板上开有多个等距排列的凹槽,所述放置板上的凹槽内放置有无菌培养皿,所述灭菌箱的右侧铰接有与移动板相对应的箱门,所述灭菌箱的内壁固定有与无菌培养皿相对应的加热器,所述灭菌箱的底端固定有水箱,所述水箱的底端通过水管连接有水泵,所述水泵的输出端通过水管连接有安装板,所述安装板固定于灭菌箱的顶端,所述安装板上安装有多个等距排列的雾化喷头,所述灭菌箱的侧壁上开有排气孔。A sterilization box is fixed on the left side of the upper surface of the operation box, a moving plate is fixed on the top of the support rod, a placing plate is placed on the upper surface of the moving plate, and a plurality of equidistantly arranged grooves are opened on the placing plate , a sterile culture dish is placed in the groove on the placement plate, the right side of the sterilization box is hinged with a box door corresponding to the moving plate, and the inner wall of the sterilization box is fixed with a sterile culture dish For the corresponding heater, a water tank is fixed at the bottom end of the sterilization box, a water pump is connected to the bottom end of the water tank through a water pipe, and a mounting plate is connected to the output end of the water pump through a water pipe, and the mounting plate is fixed to the sterilizer. At the top of the bacteria box, a plurality of equidistantly arranged atomizing nozzles are installed on the mounting plate, and an exhaust hole is opened on the side wall of the sterilization box.
优选的,所述操作箱的外部外壁固定有安装箱,所述电机位于安装箱内部。Preferably, an installation box is fixed on the outer wall of the operation box, and the motor is located inside the installation box.
优选的,所述移动板远离支撑杆的一侧固定有两个对称设置的滚轮。Preferably, two symmetrically arranged rollers are fixed on the side of the moving plate away from the support rod.
优选的,所述无菌培养皿为长条型,且无菌培养皿两侧对称的设置有侧耳。Preferably, the sterile culture dish is a long strip, and lateral ears are symmetrically arranged on both sides of the sterile culture dish.
优选的,所述第一滑块、第二滑块均为T型滑块。Preferably, the first sliding block and the second sliding block are both T-shaped sliding blocks.
优选的,所述排气孔有多个,且在灭菌箱的外壁等距排列。Preferably, there are multiple exhaust holes, and they are arranged at equal distances on the outer wall of the sterilization box.
本实用新型的有益效果为:The beneficial effects of the present utility model are:
1、本实用新型中,通过操作箱、齿轮、转轴、齿条、第二移动杆、第一滑槽、第一移动杆、第一滑块、第二滑槽、第二滑块、支撑杆、槽口、滚轮、移动板的配合使用,方便对灭菌后放置板内的培养基从灭菌箱内拿取出来,然后将待灭菌的培养基进行重新装配送入灭菌箱内灭菌,1. In this utility model, through the operation box, gear, rotating shaft, rack, second moving rod, first sliding groove, first moving rod, first sliding block, second sliding groove, second sliding block, support rod It is convenient to take out the medium placed in the plate after sterilization from the sterilization box, and then reassemble the medium to be sterilized and send it to the sterilization box for sterilization. bacteria,
2、通过水泵、雾化喷头的配合使用,采用蒸汽式加热高温灭菌更加稳定,且不容易培养基造成损坏。2. Through the combined use of water pump and atomizing nozzle, steam heating and high temperature sterilization is more stable, and it is not easy to damage the culture medium.
附图说明Description of drawings
图1为本实用新型的一种无菌培养基的灭菌装置的结构示意图。FIG. 1 is a schematic structural diagram of a sterilization device for a sterile culture medium according to the present invention.
图2为本实用新型的一种无菌培养基的灭菌装置的安装箱的结构示意图。FIG. 2 is a schematic structural diagram of an installation box of a sterilization device for a sterile culture medium according to the present invention.
图3为本实用新型的一种无菌培养基的灭菌装置的第二滑块的结构示意图。3 is a schematic structural diagram of a second slider of a sterilization device for sterile culture medium of the present invention.
图4为本实用新型的一种无菌培养基的灭菌装置的无菌培养皿的结构示意图。4 is a schematic structural diagram of a sterile petri dish of a sterilization device for a sterile culture medium of the present invention.
图5为本实用新型的一种无菌培养基的灭菌装置的移动板的底端的结构示意图。5 is a schematic structural diagram of the bottom end of a moving plate of a sterilization device for sterile culture medium according to the present invention.
图6为本实用新型的一种无菌培养基的灭菌装置的主视图。6 is a front view of a sterilization device for a sterile culture medium of the present invention.
图中标号:1操作箱、3齿轮、4转轴、5齿条、6第二移动杆、7第一滑槽、8第一移动杆、9第一滑块、10第二滑槽、11第二滑块、12支撑杆、13槽口、14滚轮、15放置板、16无菌培养皿、17加热器、18箱门、19灭菌箱、20排气孔、21水箱、22水泵、23安装板、24雾化喷头、25电机、26安装箱、27移动板。Labels in the figure: 1 operation box, 3 gear, 4 shaft, 5 rack, 6 second moving rod, 7 first chute, 8 first moving rod, 9 first sliding block, 10 second chute, 11 first sliding
具体实施方式Detailed ways
下面将结合本实用新型实施例中的附图,对本实用新型实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本实用新型一部分实施例,而不是全部的实施例。The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the implementations. example.
参照图1-6,一种无菌培养基的灭菌装置,包括操作箱1,操作箱1的内腔内壁中心处通过轴承转动连接有转轴4,所述转轴4的表面套接有齿轮3,所述转轴4的一端贯穿操作箱1并延伸至操作箱1的外部,转轴4的延伸端连接有电机25,齿轮3的顶端啮合有齿条5,齿条5顶部的两侧分别固定连接有第一移动杆8和第二移动杆6,第一移动杆8、第二移动杆6的顶端分别固定有第一滑块9、第二滑块11,操作箱1内腔的顶部开设有与第一滑块9、第二滑块11配合使用的第一滑槽7、第二滑槽10,第一滑块9、第二滑块11与第一滑槽7、第二滑槽10的内壁滑动连接,操作箱1的顶部开设有位于第二滑槽10上方的槽口13,第二滑块11的顶端固定有支撑杆12;1-6, a sterilization device for sterile culture medium, comprising an operation box 1, a rotating shaft 4 is rotatably connected to the center of the inner wall of the inner cavity of the operation box 1 through a bearing, and a gear 3 is sleeved on the surface of the rotating shaft 4 , one end of the rotating shaft 4 penetrates the operation box 1 and extends to the outside of the operation box 1, the extension end of the rotating shaft 4 is connected with the motor 25, the top of the gear 3 is engaged with a rack 5, and the two sides of the top of the rack 5 are respectively fixedly connected There are a first moving rod 8 and a second moving rod 6, the tops of the first moving rod 8 and the second moving rod 6 are respectively fixed with a first sliding block 9 and a second sliding
操作箱1的上表面左侧固定有灭菌箱19,支撑杆12的顶端固定有移动板27,移动板27上表面放置有放置板15,放置板15上开有多个等距排列的凹槽,放置板15上的凹槽内放置有无菌培养皿16,灭菌箱19的右侧铰接有与移动板27相对应的箱门18,灭菌箱19的内壁固定有与无菌培养皿16相对应的加热器17,灭菌箱19的底端固定有水箱21,水箱21的底端通过水管连接有水泵22,水泵22的输出端通过水管连接有安装板23,安装板23固定于灭菌箱19的顶端,安装板23上安装有多个等距排列的雾化喷头24,灭菌箱19的侧壁上开有排气孔20,操作箱1的外部外壁固定有安装箱26,电机25位于安装箱26内部,移动板27远离支撑杆12的一侧固定有两个对称设置的滚轮14,无菌培养皿16为长条型,且无菌培养皿16两侧对称的设置有侧耳,第一滑块9、第二滑块11均为T型滑块,排气孔20有多个,且在灭菌箱19的外壁等距排列。A
工作原理:working principle:
本实用新型使用时,首先将培养基原料放在无菌培养皿16中完成配制,然后通过侧耳拿取无菌培养皿16,并将无菌培养皿16放置在放置板15表面的凹槽中,然后打开箱门18,启动电机15,电机15带动转轴4转动,转轴4带动齿轮3转动,齿轮3带动齿条向左移动,齿条3通过第二移动杆6带动第二滑块11移动,第二滑块11通过支撑杆12带动移动板27移动,通过移动板27将放置板15上的无菌培养皿16移动至灭菌箱19内,启动水泵22通过雾化喷头24对无菌培养皿16内的培养基进行喷水,启动加热器17对培养基进行高温灭菌处理,雾化后的蒸汽通过20排出,然后在启动电机15,电机15带动转轴4转动,转轴4带动齿轮3转动,齿轮3带动齿条向右移动,齿条3通过第二移动杆6带动第二滑块11移动,第二滑块11通过支撑杆12带动移动27移动,通过移动27将放置板15上的无菌培养皿16移动至19外部,将灭菌后的培养基拿取出来进行保存。When the utility model is used, the culture medium raw material is firstly placed in the
以上所述,仅为本实用新型较佳的具体实施方式,但本实用新型的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本实用新型揭露的技术范围内,根据本实用新型的技术方案及其实用新型构思加以等同替换或改变,都应涵盖在本实用新型的保护范围之内。The above description is only a preferred embodiment of the present invention, but the protection scope of the present invention is not limited to this. Equivalent replacement or modification of the new technical solution and its utility model concept shall be included within the protection scope of the present utility model.
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CN112425509A (en) * | 2020-12-17 | 2021-03-02 | 青州市亚泰农业科技有限公司 | Sterile culture medium for waxy phalaenopsis industrial tissue culture |
CN113070116A (en) * | 2021-04-26 | 2021-07-06 | 陈疑 | Operation panel convenient to deposit anaerobic culture medium |
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CN112425509A (en) * | 2020-12-17 | 2021-03-02 | 青州市亚泰农业科技有限公司 | Sterile culture medium for waxy phalaenopsis industrial tissue culture |
CN113070116A (en) * | 2021-04-26 | 2021-07-06 | 陈疑 | Operation panel convenient to deposit anaerobic culture medium |
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