CN113946045A - Glass slide and use method thereof - Google Patents
Glass slide and use method thereof Download PDFInfo
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- CN113946045A CN113946045A CN202111196106.XA CN202111196106A CN113946045A CN 113946045 A CN113946045 A CN 113946045A CN 202111196106 A CN202111196106 A CN 202111196106A CN 113946045 A CN113946045 A CN 113946045A
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- 239000011521 glass Substances 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000009194 climbing Effects 0.000 claims abstract description 29
- 238000001514 detection method Methods 0.000 claims abstract description 20
- 239000000523 sample Substances 0.000 claims description 122
- 238000011534 incubation Methods 0.000 claims description 13
- 238000003860 storage Methods 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 241000219098 Parthenocissus Species 0.000 claims description 8
- 239000003292 glue Substances 0.000 claims description 4
- 239000012470 diluted sample Substances 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 238000001890 transfection Methods 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of glass slides, and particularly discloses a glass slide and a use method of the glass slide. The glass slide comprises a bottom plate, wherein a first groove is formed by inwards recessing the upper surface of the bottom plate, and a sample adding groove and an article placing groove are formed by inwards recessing the bottom of the first groove; put the thing recess and be provided with a plurality ofly, a plurality ofly put the even setting of thing recess in the periphery of application of sample recess, every puts all through first passageway level intercommunication between thing recess and the application of sample recess. According to the invention, a worker can add a sample into the sample adding groove to enable the sample to flow into the object placing groove from the first channel, so that the sample can be fully distributed on the climbing sheet in the object placing groove, and further testing is facilitated; this process is easy and simple to handle, only needs when adding the sample add in the sample groove, and then can not appear leaking the circumstances of adding or adding the sample more, has improved the detection efficiency of sample.
Description
Technical Field
The invention relates to the technical field of glass slides, in particular to a glass slide and a use method of the glass slide.
Background
When the cell substrate is used as a basic method for sample detection, the sample is required to be added into the hole where each slide is located when the sample is added, the operation of the process is complicated, and the detection efficiency is further influenced; moreover, the phenomenon of adding missing or adding more samples is easy to occur in the process of adding samples, individual differences exist in the operation process, the influence on the factors of the test result is large, the same item and the same test method are often generated, the test results of two laboratories are different, and clinical misdiagnosis is easy to cause when a clinician combines the test results.
Disclosure of Invention
The invention aims to provide a glass slide and a use method of the glass slide, which are used for solving the problems of complicated operation and low efficiency of a detection process caused by the fact that samples need to be added into holes of each slide when the samples are detected in the prior art, and simultaneously reducing the phenomena of addition omission or addition.
In order to achieve the purpose, the invention adopts the following technical scheme:
in one aspect, the present invention provides a slide comprising:
the upper surface of the bottom plate is recessed inwards to form a first groove, and the bottom of the first groove is recessed inwards to form a sample adding groove and an object placing groove; the sample adding groove is used for adding a sample, and the article placing groove is used for placing a climbing sheet; the article placing grooves are provided with a plurality of articles, the article placing grooves are uniformly arranged at the periphery of the sample adding groove, and each article placing groove is horizontally communicated with the sample adding groove through a first channel, so that the samples in the sample adding grooves can flow into the article placing grooves through the first channels.
Furthermore, the height of the bottom of the sample adding groove is lower than that of the bottom of the object placing groove, and the sample adding groove is arranged in the middle of the first groove.
Further, a second channel is arranged between two adjacent article placing grooves, and the second channels are horizontally communicated with the two adjacent article placing grooves.
Furthermore, the bottom height of the second channel is higher than the groove bottom height of the object placing groove.
Further, the bottom plate is of a polygonal structure, and a chamfer is arranged at one of the top corners of the bottom plate.
Further, the bottom plate is of a rectangular structure, and a holding part is arranged at one end of the bottom plate along the length direction.
Furthermore, the glass slide also comprises a cover plate, and the cover plate is covered in the first groove and used for pressing the climbing piece.
Further, a stepped groove is formed by the upper surface of the bottom plate sinking inwards, the stepped groove comprises a first groove at the lower part and a second groove at the upper part, and a step is arranged between the first groove and the second groove; the sample adding groove and the article placing groove are both arranged at the bottom of the first groove;
the cover plate comprises a cover plate body and a limiting part convexly arranged on the lower surface of the cover plate body, the cover plate body is covered in the second groove and supported on the step to block the sample in the object placing groove from flowing out to the outside; the limiting part is covered in the first groove.
Furthermore, the cover plate is provided with a first through hole, and the first through hole and the sample adding groove are oppositely arranged and communicated in the vertical direction, so that the sample can be added into the sample adding groove through the first through hole.
Further, a second through hole is formed in the cover plate, and the second through hole and the accommodating groove are oppositely arranged in the vertical direction and are communicated with each other so as to release gas in the accommodating groove.
In another aspect, the present invention provides a method of using a slide glass, the method of using the slide glass is performed using the slide glass described above, and the method includes the following steps:
s1, fixing the climbing piece: transferring the creeper with cells after transfection or drug treatment of the recombinant vector into the article placing groove of the glass slide, and before transferring the creeper, dripping glue on the lower surface of the creeper for fixing the creeper in the article placing groove;
s2, incubation of sample: dropping a sufficient diluted sample into the sample adding groove, so that the sample in the sample adding groove can flow into the object placing groove from the first channel, and placing the whole glass slide into a constant-temperature and moisture-preserving incubator for incubation of the sample;
s3, rinsing: sucking away the sample in the sample adding groove, and then dropwise adding sufficient rinsing liquid into the sample adding groove for rinsing, wherein the rinsing frequency is set according to requirements;
s4, incubation secondary antibody: sucking away the rinsing liquid in the sample adding groove, then dropwise adding enough secondary antibody into the sample adding groove, and placing the whole glass slide in a constant-temperature and moisture-preserving incubator to perform secondary antibody incubation;
s5, rinsing: absorbing the second antibody in the sample adding groove, then dropwise adding sufficient rinsing liquid into the sample adding groove for rinsing, setting the rinsing times according to requirements, and finally filling the rinsing liquid into the storage groove in the glass slide;
s6, observation result: the slide glass is translated to a stage of a fluorescence microscope, and the result of the detection is observed under the fluorescence microscope through the back surface of the base plate.
The invention has the beneficial effects that:
the glass slide comprises a bottom plate, wherein a first groove is formed by inwards recessing the upper surface of the bottom plate, a plurality of object placing grooves are formed by inwards recessing the bottom of the first groove, an object adding groove and an object placing groove are formed by inwards recessing the bottom of the first groove, the object placing grooves are uniformly arranged at the periphery of the object adding groove, and each object placing groove is horizontally communicated with the object adding groove through a first channel; this process is easy and simple to handle, only needs when adding the sample add in the sample groove, and then can not appear leaking the circumstances of adding or adding the sample more, has improved the detection efficiency of sample.
The use method of the glass slide is completed by utilizing the glass slide, and the use method of the glass slide can accurately detect the cultivation condition of a sample; the use method of the glass slide is simple and convenient, the detection efficiency of the sample is high, and meanwhile, the referential performance of the detected result is high.
Drawings
FIG. 1 is a schematic view of a slide according to one embodiment of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1 at A;
FIG. 3 is a schematic view of a cover plate according to an embodiment of the present invention;
FIG. 4 is a schematic view of a slide according to a second embodiment of the present invention;
fig. 5 is a schematic structural diagram of a cover plate according to a second embodiment of the present invention.
In the figure:
1. a base plate; 11. a first groove; 111. a sample adding groove; 112. a storage groove; 113. a first channel; 114. a second channel; 12. a second groove; 13. chamfering; 14. a holding section; 115. a first boss portion; 2. a cover plate; 21. a first through hole; 22. a second through hole; 23. a second boss portion; 24. a cover plate body; 25. a limiting part; 3. and (5) climbing sheets.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Where the terms "first position" and "second position" are two different positions, and where a first feature is "over", "above" and "on" a second feature, it is intended that the first feature is directly over and obliquely above the second feature, or simply means that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
Example one
The present embodiment provides a slide glass for performing specimen testing.
As shown in FIGS. 1-2, the slide includes a base plate 1 and a cover plate 2. The upper surface of the bottom plate 1 is recessed inwards to form a first groove 11, the bottom of the first groove 11 is recessed inwards to form a sample adding groove 111 and an article placing groove 112, meanwhile, a plurality of article placing grooves 112 are arranged, the plurality of article placing grooves 112 are uniformly arranged on the periphery of the sample adding groove 111, and each placing groove 112 is horizontally communicated with the sample adding groove 111 through a first channel 113, wherein, the sample adding groove 111 is used for adding samples, the object placing groove 112 is used for placing the climbing sheet 3, so that the sample loaded in the loading groove 111 can flow into the holding groove 112 through the first channel 113, so that the climbing sheets 3 in the storage groove 112 can be fully distributed with samples, cells on the climbing sheets 3 can be conveniently placed in the samples for further testing, and when the samples are independently added on each climbing sheet 3, the impact force in the adding process causes the cells on the climbing sheet 3 to fall off, so that the detection result fails; and the middle position department of first recess 11 is located to application of sample recess 111, and then conveniently evenly sets up the same size in application of sample recess 111's periphery and puts thing recess 112, the even inflow of the sample of being convenient for and the staff to the detection of sample. Optionally, the surfaces of the placement recesses 112 are treated with a hydrophilic coating to promote uniform flow of sample from the sample application recess 111 into each placement recess 112.
The process is simple and convenient to operate, and the sample is only added into the sample adding groove 111 when being added, so that the condition of adding missing or adding more samples is avoided, and the detection efficiency of the sample is improved; wherein, in first recess 11 is located to apron 2 lid to be used for compressing tightly climbing piece 3, prevent that climbing piece 3 from appearing floating the removal in putting thing recess 112. Optionally, glue may be used to secure the slide 3 within the placement groove 112 to prevent movement of the slide 3 during subsequent sample addition or testing. Further optionally, the number of the storage grooves 112 and the number of the crawlers 3 may be increased or decreased according to the number of the detection items, so as to be suitable for various detection situations, meanwhile, a plurality of first grooves 11 may be formed in one glass slide, each first groove 11 is provided with a sample adding groove 111 and a storage groove 112, which are the same as those in the above description, and then, by adding a sample into each sample adding groove 111, the processing speed of the sample may be greatly increased, and the loss of the sample may also be reduced.
Further, be provided with first through-hole 21 on apron 2, and first through-hole 21 and application of sample recess 111 set up relatively and communicate in vertical direction, and then locate 2 lids of apron in first recess 11 back, can make the sample add by first through-hole 21 and put in the pressurization recess, thereby the sample of interpolation can follow the slow inflow in application of sample recess 111 and put in the recess 112, and will put the interior climbing piece 3 cover of recess 112, and simultaneously, after covering apron 2, the inside formation cavity of slide, so that the sample more evenly flows in each and puts in the recess 112. Optionally, still be provided with second through-hole 22 on apron 2, second through-hole 22 with put thing recess 112 and set up and communicate relatively in vertical direction, and then at the in-process that adds the sample, can release the gas of putting the production in the thing recess 112, avoid putting the production bubble in the thing recess 112, further influence the detection effect, simultaneously, can make things convenient for the staff to observe the sample through microscope via second through-hole 22.
As shown in fig. 2, the bottom height of the sample adding groove 111 is lower than the bottom height of the object placing groove 112, and then when the sample in the sample adding groove 111 is sucked away by the dropper, the sample in the object placing groove 112 can flow into the sample adding groove 111, so that the sample in the object placing groove 112 can be sucked away by the worker, and the problem of low efficiency caused by independent suction of the sample in each object placing groove 112 is avoided. Specifically, the outer periphery of the groove bottom of the sample addition groove 111 is inclined upward toward the outer periphery of the sample addition groove 111 along an inclination angle of 5 ° to 10 ° with the horizontal direction.
Further, a second channel 114 is arranged between two adjacent article placing grooves 112, and the second channel 114 horizontally communicates the two adjacent article placing grooves 112, so that after the bottom plate 1 is covered with the cover plate 2, a sample dropped into the sample adding groove 111 can flow into the article placing groove 112, and then flows into the other adjacent article placing groove 112 through the article placing groove 112, so that in the process of adding the sample, the sample can more uniformly flow into each article placing groove 112. Meanwhile, because the first channel 113 and the second channel 114 are arranged, the first protruding portion 115 is reserved between the two article placing grooves 112, the first protruding portion 115 can not only play a role in isolating the two article placing grooves 112, but also can limit the movement of the climbing sheet 3, and when the climbing sheet 3 is placed into the article placing grooves 112, the first protruding portion 115 can limit the climbing sheet 3, and guide the climbing sheet 3 to fall into the article placing grooves 112. Wherein, the bottom height of second passageway 114 is higher than the tank bottom height of putting thing recess 112 to climbing piece 3 can be restricted to put in thing recess 112, avoids climbing piece 3 to produce along the horizontal direction and rocks, influences the detection of sample.
Optionally, the bottom plate 1 is polygonal structure, and one of them apex angle department in a plurality of apex angles of bottom plate 1 is provided with chamfer 13, and then chamfer 13 can carry out the reference of fixing a position to putting thing recess 112 to make things convenient for the staff to distinguish every position of putting thing recess 112, prevent the misplacement of climbing piece 3 and the misplacement of sample.
As shown in fig. 3, the bottom of the cover plate 2 is provided with a second protruding portion 23 in the vertical direction, the second protruding portion 23 can be disposed in the second channel 114 and attached to the side wall of the second channel 114, and meanwhile, the second protruding portion 23 extends in the horizontal direction, so that the portion of the second protruding portion 23 extending in the horizontal direction can compress the climbing sheet 3, and the climbing sheet 3 is prevented from floating after the storage groove 112 is filled with the sample; wherein, apron 2 lid is located bottom plate 1 back, and second bellying 23 and second passageway 114 have the space in vertical direction, and then adjacent two samples of putting in the recess 112 still can circulate each other, do not receive the separation.
The embodiment also provides a use method of the glass slide, which is completed by using the glass slide, and comprises the following steps:
s1, fixing the climbing piece: transferring the cell-stored climbing sheet 3 after transfection or drug treatment of the recombinant vector into the storage groove 112 of the glass slide, and before transferring the climbing sheet 3, dripping glue on the lower surface of the climbing sheet 3 to fix the climbing sheet 3 in the storage groove 112;
s2, incubation of sample: dropping a sufficient diluted sample into the sample adding groove 111, so that the sample in the sample adding groove 111 can flow into the article placing groove 112 through the first channel 113, and placing the whole glass slide into a constant-temperature and moisture-preserving incubator for incubation of the sample;
s3, rinsing: sucking away the sample in the sample adding groove 111, then dropwise adding sufficient rinsing liquid into the sample adding groove 111 for rinsing, wherein the rinsing frequency is set according to requirements;
s4, incubation secondary antibody: sucking away the rinsing liquid in the sample adding groove 111, then dropwise adding sufficient secondary antibody into the sample adding groove 111, and then placing the whole glass slide into a constant-temperature and moisture-preserving incubator to perform secondary antibody incubation;
s5, rinsing: absorbing the second antibody in the sample adding groove 111 away, then dropwise adding sufficient rinsing liquid into the sample adding groove 111 for rinsing, setting the rinsing times according to requirements, and finally filling the rinsing liquid into the article placing groove 112 in the fully-loaded glass;
s6, observation result: the slide glass is translated to the stage of a fluorescence microscope, and the detection result is observed under the fluorescence microscope through the back surface of the base plate (1).
The use method of the glass slide provided by the embodiment can accurately detect the incubation condition of the sample; the use method of the glass slide is simple and convenient, the detection efficiency of the sample is high, and meanwhile, the referential performance of the detected result is high.
Example two
The present embodiment also provides a slide glass that can also be used for performing specimen testing.
The slide glass provided by the embodiment is basically the same as the slide glass in the first embodiment, except that, as shown in fig. 4-5, a stepped groove is formed by inwards recessing the upper surface of the bottom plate 1 in the embodiment, the stepped groove comprises a first groove 11 at the lower part and a second groove 12 at the upper part, and a step is arranged between the first groove 11 and the second groove 12; the sample adding groove 111 and the article placing groove 112 are both arranged at the bottom of the first groove 11; meanwhile, the cover plate 2 comprises a cover plate body 24 and a limiting part 25 convexly arranged on the lower surface of the cover plate body 24, the cover plate body 24 is covered in the second groove 12 and supported on the step to block the sample in the object placing groove 112 from flowing out to the outside, so that the influence on the detection of the sample and the increase of the detection cost are avoided; spacing portion 25 lid is located in first recess 11 to can prevent that the shake from appearing along the horizontal direction in apron 2, cause the influence to the detection of sample.
Optionally, the bottom plate 1 is of a rectangular structure, one end of the bottom plate 1 in the length direction is provided with a holding portion 14, and the holding portion 14 can facilitate a worker to take a slide. Specifically, the holding part 14 is a boss protruding from one end of the bottom plate 1 along the length direction, and the height of the upper surface of the holding part 14 is lower than that of the upper surface of the step part 12, so that the worker can distinguish the holding part 14. During specific implementation, the size of the glass slide can be set to be the same as that of a conventional and universal glass slide, so that the glass slide is not required to be taken out alone for observation, and the whole glass slide is directly placed on a microscope objective table. Further optionally, in this embodiment, the slide is 75x25mm in size.
This example also provides a method of using a slide glass that is the same as the method of using the slide glass of example one.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (11)
1. A slide, comprising:
the device comprises a bottom plate (1), wherein a first groove (11) is formed by inwards recessing the upper surface of the bottom plate (1), and a sample adding groove (111) and an article placing groove (112) are formed by inwards recessing the bottom of the first groove (11); the sample adding groove (111) is used for adding a sample, and the article placing groove (112) is used for placing the climbing sheet (3); put thing recess (112) and be provided with a plurality ofly, it is a plurality of put thing recess (112) are in the periphery of application of sample recess (111) evenly sets up, every put thing recess (112) with all communicate through first passageway (113) level between application of sample recess (111), so that in application of sample recess (111) the sample can by first passageway (113) flows in put in the thing recess (112).
2. The slide according to claim 1, wherein the groove bottom of the sample application groove (111) has a height lower than that of the placement groove (112), and the sample application groove (111) is provided at a middle position of the first groove (11).
3. The slide as claimed in claim 1 wherein a second channel (114) is disposed between two adjacent receiving recesses (112), the second channel (114) horizontally communicating the two adjacent receiving recesses (112).
4. The slide as claimed in claim 3, wherein the bottom height of the second channel (114) is higher than the bottom height of the placement recess (112).
5. Glass slide according to claim 1, characterized in that the base plate (1) is of polygonal configuration, one of the corners of the base plate (1) being provided with a chamfer (13).
6. Slide according to claim 1, wherein the base plate (1) is of rectangular configuration, and a grip portion (14) is provided at one end of the base plate (1) in the longitudinal direction.
7. A slide as claimed in claim 1, further comprising a cover plate (2), said cover plate (2) being housed in said first recess (11) for pressing said slide (3).
8. The slide glass according to claim 1, wherein the upper surface of the bottom plate (1) is recessed inwardly to form a stepped groove comprising a lower first groove (11) and an upper second groove (12), wherein a step is provided between the first groove (11) and the second groove (12); the sample adding groove (111) and the article placing groove (112) are both arranged at the bottom of the first groove (11);
the cover plate (2) comprises a cover plate body (24) and a limiting part (25) convexly arranged on the lower surface of the cover plate body (24), the cover plate body (24) is arranged in the second groove (12) in a covering mode and supported on the step to block the sample in the storage groove (112) from flowing out to the outside; the limiting part (25) is arranged in the first groove (11) in a covering mode.
9. The slide glass according to claim 7, wherein the cover plate (2) is provided with a first through hole (21), and the first through hole (21) is vertically opposed to and communicates with the sample addition groove (111) so that the sample can be added into the sample addition groove (111) through the first through hole (21).
10. The slide glass according to claim 9, wherein the cover plate (2) is provided with a second through hole (22), and the second through hole (22) is disposed opposite to and communicates with the housing recess (112) in the vertical direction to release the gas in the housing recess (112).
11. A method of using a slide, wherein the method of using the slide is performed using the slide of any one of claims 1 to 10, comprising the steps of:
s1, fixing the climbing piece: transferring the creeper (3) with cells stored therein after transfection or drug treatment of the recombinant vector into the storage groove (112) of the glass slide, and before transferring the creeper (3), dripping glue on the lower surface of the creeper (3) for fixing the creeper (3) in the storage groove (112);
s2, incubation of sample: dropping a sufficient amount of diluted sample into the sample adding groove (111), so that the sample in the sample adding groove (111) can flow into the storage groove (112) from the first channel (113), and placing the whole glass slide into a constant-temperature and moisture-preserving incubator for incubation of the sample;
s3, rinsing: sucking away the sample in the sample adding groove (111), and then dropwise adding sufficient rinsing liquid into the sample adding groove (111) for rinsing, wherein the rinsing times are set according to requirements;
s4, incubation secondary antibody: sucking away rinsing liquid in the sample adding groove (111), then dropwise adding sufficient secondary antibody into the sample adding groove (111), and placing the whole glass slide in a constant-temperature and moisture-preserving incubator for incubation of the secondary antibody;
s5, rinsing: absorbing the secondary antibody in the sample adding groove (111), then dropwise adding a sufficient amount of rinsing liquid into the sample adding groove (111) for rinsing, setting the rinsing times according to requirements, and finally filling the rinsing liquid into the storage groove (112) in the glass slide;
s6, observation result: the glass slide is translated to a fluorescence microscope stage, and the detection result is observed under a fluorescence microscope through the back surface of the bottom plate (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111196106.XA CN113946045A (en) | 2021-10-14 | 2021-10-14 | Glass slide and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111196106.XA CN113946045A (en) | 2021-10-14 | 2021-10-14 | Glass slide and use method thereof |
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| CN113946045A true CN113946045A (en) | 2022-01-18 |
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| CN112834763A (en) * | 2019-11-22 | 2021-05-25 | 京东方科技集团股份有限公司 | Detection chip and detection system |
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2021
- 2021-10-14 CN CN202111196106.XA patent/CN113946045A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105665044A (en) * | 2016-01-28 | 2016-06-15 | 浙江大学 | Micro-fluidic chip assembly |
| CN208984536U (en) * | 2018-10-17 | 2019-06-14 | 重庆城市管理职业学院 | Trace of liquid detects reaction chamber |
| CN209707523U (en) * | 2019-02-13 | 2019-11-29 | 天津市宝坻区人民医院 | Respiratory pathogen IgM antibody detection plate in serum |
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