CN219430014U - Independent embryo culture dish - Google Patents

Abstract

The utility model provides a stand-alone embryo culture dish, which comprises a dish body, wherein the dish body comprises a main body area and a plurality of culture bins arranged in the main body area, and any adjacent culture bins are arranged at intervals; the bottom of each cultivate the storehouse all is equipped with one and is used for placing the culture hole of embryo, and each cultivate the top of storehouse all be equipped with around this separation structure of cultivateing the storehouse setting, so that each cultivate the storehouse all has independent coverage area. According to the utility model, each culture bin has an independent coverage area, so that the culture oil in each culture bin is prevented from being mixed with each other, the independence of each culture bin is ensured, the function of identifying the loading position of the culture oil can be realized in the process of adding the culture oil, and the loading operation of the culture oil is facilitated.

Description

Independent embryo culture dish

Technical Field

The utility model relates to the technical field of assisted reproduction, in particular to a stand-alone embryo culture dish.

Background

Embryo culture refers to culturing the obtained early embryo in an in vitro artificial environment to monitor the development or the possibility of manual intervention in a certain development process, and conventional embryo culture is mostly carried out on a common culture dish and needs to be put into a special incubator for culture; embryo analysis requires manual transfer of embryos under the microscope in the atmosphere, and the embryos are returned to the incubator after microscopic examination. The operation can cause fluctuation of air pressure, temperature and humidity, increase the embryo exposure chance and influence the embryo development; moreover, the conventional embryo culture analysis and evaluation can only obtain development images at a plurality of time points, and the evaluation result has larger subjectivity and limitation due to different indexes selected in a laboratory and different time and proficiency of technicians in embryo evaluation.

At present, the time difference incubator technology can continuously photograph the embryo development process, embryologists do not need to take the embryo out of the incubator for observation, and people can intuitively and uninterruptedly observe the embryo development condition only through images in a laboratory; the whole process is free from interference and is closer to the culture condition of a parent body; meanwhile, the time difference monitoring technology enables embryo development potential assessment to be more objective and standardized, and reduces human errors.

Independent embryo culture dishes, i.e., individual embryos are placed in a single culture chamber, each of which is placed with culture fluid alone, without sharing culture fluid and nutrients produced by the embryos with the other chambers. Above the culture liquid, it is often necessary to cover a layer of culture oil in order to prevent fluid communication between the different culture liquids. The independent culture dishes of the prior art usually form a common culture oil layer above all the culture bins due to uncontrollable addition of culture oil. The common culture oil layer inevitably causes interaction and medium transfer between embryos. In order to avoid this phenomenon, when the culture oil is added to the independent embryo culture dish in the prior art, the addition amount of the culture oil in each culture bin needs to be accurately calculated, so as to prevent the occurrence of the liquid mixing of the culture oil between different culture bins due to the excessive loading of the culture oil, and further influence the culture effect of the embryo. The operation is complicated and depends on the hand feeling of doctors.

It should be noted that the information disclosed in this background section is only for enhancement of understanding of the general background of the utility model and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.

Disclosure of Invention

The utility model aims to provide a free-standing embryo culture dish which not only enables each culture bin to have an independent culture oil coverage area, but also can identify the loading position of the culture oil and prevent overload.

In order to achieve the above purpose, the utility model provides a stand alone embryo culture dish, comprising a dish body, wherein the dish body comprises a main body area and a plurality of culture bins arranged in the main body area, and any adjacent culture bins are arranged at intervals;

the bottom of each cultivate the storehouse all is equipped with one and is used for placing the culture hole of embryo, and each cultivate the top of storehouse all be equipped with around this separation structure of cultivateing the storehouse setting, so that each cultivate the storehouse all has independent coverage area.

Optionally, in some embodiments, the blocking structure above each culture bin is an annular boss disposed around the culture bin, an outer diameter of the annular boss is greater than an inner diameter of the culture bin, an outer diameter of the annular boss is 3.5mm to 3.8mm, and a height of the annular boss is 0.3mm to 0.5mm.

Optionally, in other embodiments, the barrier structure above each of the culture compartments is constituted by a grating disposed within the body region, the grating disposed around the culture compartments, the grating comprising a plurality of parallel disposed transverse grating strips and a plurality of parallel disposed longitudinal grating strips, the transverse grating strips and the longitudinal grating strips intersecting each other.

Further, small holes are formed at the connection positions of the transverse grating strips and the longitudinal grating strips.

Optionally, in the main body area, in addition to the area where the culture bin is located, a flushing bin area is further provided, which is located at one side or two opposite sides of the area where the culture bin is located, and each flushing bin area is provided with a plurality of flushing bins which are spaced from each other.

Optionally, the multiple flushing bins are respectively arranged on two sides of the multiple culture bins along the length direction of the dish body, two ends of the transverse grid bars are respectively abutted against corresponding outer side walls of the flushing bins, and two ends of the longitudinal grid bars are respectively abutted against inner walls of two sides of the main body area.

Optionally, the main part district is from top to bottom including consecutive enclose keep off, gather together slope and concave station, cultivate the storehouse with wash the storehouse and be located in the region that the concave station encloses, just cultivate the storehouse for concave station undercut sets up in order to extend to the below in main part district, wash the storehouse for concave station upward protrusion sets up in order to extend to gather together in the region that the slope encloses.

Optionally, the body region includes one or more of the following features:

the inner peripheral dimension of the gathering slope is gradually reduced from top to bottom, and the inner wall of the gathering slope is obliquely arranged at an angle of 5-45 degrees with the horizontal direction;

the inner peripheral dimension of the enclosure is gradually reduced from top to bottom, and the inner wall of the enclosure is obliquely arranged at an angle of more than 0 degrees and less than or equal to 30 degrees with the vertical direction;

the concave table comprises an inner wall in the vertical direction and an inner wall in the horizontal direction, wherein the inner wall is arranged at an angle of 90-110 degrees, and the depth of the concave table is 1-2 mm.

Optionally, the inner peripheral dimension of the flushing bin is gradually reduced from top to bottom, and the inner wall of the flushing bin is obliquely arranged at an angle of 10-60 degrees with the vertical direction.

Optionally, the outer bottom wall of the culture bin is provided with a supporting table extending downwards, and the bottom of the supporting table is provided with a first identifier for identifying the culture bin.

Optionally, the dish comprises one or more of the following features:

the distance between the central lines of two adjacent culture bins is 6.0 mm-6.5 mm;

a second identifier for identifying the flushing bin is arranged in the lower bottom view direction of the flushing bin;

The top of the dish body is provided with a handle, the handle is positioned outside the main body area, the handle is upwards arranged in a protruding mode relative to the main body area, the upper surface of the handle is arranged in a circular arc shape, and/or a first sand grinding layer is arranged on the outer side of the handle;

the top of the dish body is also provided with a mark pasting area, the mark pasting area is positioned outside the main body area, the mark pasting area is obliquely arranged at an angle of 10-45 degrees with the horizontal direction, and the mark pasting area is provided with a second sand grinding layer.

Optionally, the inner diameter of the culture hole is gradually reduced from top to bottom, the maximum diameter of the culture hole is 1.05 mm-1.08 mm, the minimum diameter of the culture hole is 0.2 mm-0.25 mm, and the depth of the culture hole is 0.50 mm-0.55 mm.

Optionally, the diameter of the inner cavity of the culture bin is 3.2-3.6 mm, and the depth of the inner cavity of the culture bin is 2.35-2.65 mm.

Optionally, the embryo culture dish further comprises a dish cover for covering the body area in use, the dish cover having one or more of the following features:

a gap is arranged between the dish cover and the main body area;

an anti-collision stop block is arranged on the outer surface of the top end of the dish cover;

The inner surface of the top end of the dish cover is provided with a limit stop;

and an anti-slip piece is arranged on the peripheral side wall of the dish cover.

Compared with the prior art, the independent embryo culture dish provided by the utility model has the following advantages:

the independent embryo culture dish provided by the utility model comprises a dish body, wherein the dish body comprises a main body area and a plurality of culture bins arranged in the main body area, and any adjacent culture bins are arranged at intervals;

the bottom of each cultivate the storehouse all is equipped with one and is used for placing the culture hole of embryo, and each cultivate the top of storehouse all be equipped with around this separation structure of cultivateing the storehouse setting, so that each cultivate the storehouse all has independent coverage area. Therefore, through the blocking structure, each culture bin has an independent coverage area, so that the culture oil in each culture bin is prevented from being mixed with each other, the independence of each culture bin is ensured, the function of marking the loading position of the culture oil can be achieved in the process of adding the culture oil, the loading operation of the culture oil is facilitated, and the overload of the loading amount of the culture oil is effectively prevented.

Drawings

FIG. 1 is a schematic perspective view of a free-standing embryo culture dish according to a first embodiment of the present utility model at a main viewing angle;

FIG. 2 is a schematic perspective view of the dish body shown in FIG. 1 at a bottom view angle;

FIG. 3 is a front view of the bowl shown in FIG. 1;

FIG. 4 is a top view of the dish shown in FIG. 1;

FIG. 5 is a bottom view of the dish shown in FIG. 1;

FIG. 6 is a cross-sectional view of the bowl shown in FIG. 4 taken along the A-A plane;

FIG. 7 is a cross-sectional view of the bowl shown in FIG. 4 taken along the B-B plane;

FIG. 8 is a schematic perspective view of a free-standing embryo culture dish body according to a second embodiment of the present utility model at a main viewing angle;

FIG. 9 is a schematic perspective view of the dish body shown in FIG. 8 in a bottom view;

FIG. 10 is a front view of the dish shown in FIG. 8;

FIG. 11 is a top view of the dish shown in FIG. 8;

FIG. 12 is a bottom view of the dish shown in FIG. 8;

FIG. 13 is a cross-sectional view of the bowl of FIG. 11 taken along the C-C plane;

FIG. 14 is a cross-sectional view of the bowl of FIG. 11 taken along the D-D plane;

FIG. 15 is a front view of a cover of a freestanding embryo culture dish provided in an embodiment of the utility model;

FIG. 16 is a cross-sectional view of the capsule shown in FIG. 15;

fig. 17 is a top view of the capsule shown in fig. 15.

Wherein, the reference numerals are as follows:

a dish body-100; a body region-110; a fence-111; gathering slope-112; a concave table-113; culturing cabin-120; culture well-121; flushing bin-130; an annular boss-140; a handle-150; an identification paste area-160; a support stand-170; grid-180; transverse grid strips-181; longitudinal grid strips-182; small holes-183;

dish cover-200; limit stops-210; bump stop-220; an anti-slip member-230;

a first identifier-310; a second identifier-320.

Detailed Description

The utility model is described in further detail below with reference to the drawings and specific examples. The advantages and features of the present utility model will become more apparent from the following description. It should be noted that the drawings are in a very simplified form and are all to a non-precise scale, merely for the purpose of facilitating and clearly aiding in the description of embodiments of the utility model. For a better understanding of the utility model with objects, features and advantages, refer to the drawings. It should be understood that the structures, proportions, sizes, etc. shown in the drawings are shown only in connection with the present disclosure for the understanding and reading of the present disclosure, and are not intended to limit the scope of the utility model, which is defined by the appended claims, and any structural modifications, proportional changes, or dimensional adjustments, which may be made by the present disclosure, should fall within the scope of the present disclosure under the same or similar circumstances as the effects and objectives attained by the present utility model. Specific design features of the utility model disclosed herein, including for example, specific dimensions, orientations, positions, and configurations, will be determined in part by the specific intended application and use environment. In the embodiments described below, the same reference numerals are used in common between the drawings to denote the same parts or parts having the same functions, and the repetitive description thereof may be omitted. In this specification, like reference numerals and letters are used to designate like items, and thus once an item is defined in one drawing, no further discussion thereof is necessary in subsequent drawings. Additionally, if a method described herein comprises a series of steps, and the order of the steps presented herein is not necessarily the only order in which the steps may be performed, and some of the described steps may be omitted and/or some other steps not described herein may be added to the method.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element. The singular forms "a," "an," and "the" include plural referents, the term "or" is generally used in the sense of comprising "and/or" and the term "several" is generally used in the sense of comprising "at least one," the term "at least two" is generally used in the sense of comprising "two or more," and the term "first," "second," "third," are for descriptive purposes only and are not to be construed as indicating or implying any relative importance or number of features indicated.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and to simplify the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "secured" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

Furthermore, in the description of the present specification, reference to the terms "one embodiment," "some embodiments," "implementation," "particular implementations," or "some implementations," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or implementation is included in at least one embodiment or implementation of the present utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or implementation. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or implementations. Furthermore, the different embodiments or implementations described in this specification and the features of the different embodiments or implementations may be combined and combined by persons skilled in the art without contradiction.

The core idea of the utility model is to provide a free-standing embryo culture dish, which not only can enable each culture bin to have an independent culture oil coverage area, but also can identify the loading position of the culture oil to prevent overload. It should be noted that, as those skilled in the art can understand, the term "plurality" herein includes two cases.

In order to achieve the above-mentioned idea, the utility model provides a stand alone embryo culture dish, comprising a dish body, wherein the dish body comprises a main body area and a plurality of culture bins arranged in the main body area, and any adjacent culture bins are arranged at intervals; the bottom of each cultivate the storehouse all is equipped with one and is used for placing the culture hole of embryo, and each cultivate the top of storehouse all be equipped with around this separation structure of cultivateing the storehouse setting, so that each cultivate the storehouse all has independent coverage area. Therefore, through the blocking structure, each culture bin has an independent coverage area, so that the culture oil in each culture bin is prevented from being mixed with each other, the independence of each culture bin is ensured, the function of marking the loading position of the culture oil can be achieved in the process of adding the culture oil, the loading operation of the culture oil is facilitated, and the overload of the loading amount of the culture oil is effectively prevented.

The independent embryo culture dish provided by the utility model is described in detail below through examples one to three.

Example 1

Referring to fig. 1 to 7, fig. 1 schematically shows a schematic perspective view of a vessel body of a free-standing embryo culture vessel according to a first embodiment of the present utility model at a main viewing angle; FIG. 2 schematically shows a perspective view of the dish of FIG. 1 at a bottom view; FIG. 3 schematically illustrates a front view of the dish shown in FIG. 1; FIG. 4 schematically shows a top view of the dish shown in FIG. 1; FIG. 5 schematically illustrates a bottom view of the dish of FIG. 1; FIG. 6 schematically shows a cross-sectional view of the bowl of FIG. 1 along the A-A plane; FIG. 7 schematically shows a cross-sectional view of the dish of FIG. 1 along the B-B plane.

As shown in fig. 1, 4 and 6, the independent embryo culture dish provided in this embodiment includes a dish body 100, where the dish body 100 includes a main body area 110 and a plurality of culture bins 120 disposed in the main body area 110, preferably, in the main body area 110, in addition to the area where the culture bins 120 are disposed, a flushing bin area disposed on one side or two opposite sides of the area where the culture bins 120 are disposed, each flushing bin area is provided with a plurality of flushing bins 130 spaced apart from each other, and any adjacent culture bins 120 are spaced apart from each other, that is, the inner cavities of the plurality of culture bins 120 are spaced apart from each other, the culture bins 120 and the flushing bins 130 are spaced apart from each other, and the flushing bins 130 are disposed on one side of the inner side of the main body area 110; or two rows, respectively disposed on both sides of the inner side of the body region 110. As shown in fig. 4, a culture hole 121 for placing embryos is formed at the bottom of each culture bin 120, and an annular boss 140 disposed around the culture bin 120 is formed at the top of each culture bin 120 to form the blocking structure above the culture bin 120, that is, the blocking structure above each culture bin 120 is the annular boss 140 disposed around the culture bin 120, and the outer diameter of the annular boss 140 is larger than the inner diameter of the culture bin 120. Therefore, in this embodiment, by disposing the annular boss 140 on the top of the culture bin 120 to form the blocking structure, not only each culture bin 120 has an independent coverage area, but also the annular boss 140 is fixedly connected with the top of the culture bin 120, so that the function of identifying the loading position of the culture oil can be better played in the process of adding the culture oil; meanwhile, since the blocking structures corresponding to each of the culture bins 120 are independently arranged at intervals, when the culture oil added in a certain culture bin 120 exceeds the top of the corresponding blocking structure, the overflowed culture oil can be ensured to directly flow into the area surrounded by the concave-convex surface of the main body area 110, and cannot flow into other culture bins 120, so that the occurrence of liquid mixing between the culture bins 120 is further prevented. In addition, since the annular boss 140 is relatively easy to process, the processing cost of the freestanding embryo culture dish provided by the utility model can be further reduced by adopting the annular boss 140 as a barrier structure.

It should be noted that, although the embodiment is described by taking the example that the dish body 100 includes 16 culture bins 120 and 6 washing bins 130 as an example, as those skilled in the art will appreciate, this is not a limitation of the present utility model, and in other embodiments, the number of the culture bins 120 may be more than 16 or less than 16, and the number of the washing bins 130 may be less than 6 or more than 6. In addition, as will be appreciated by those skilled in the art, the dish body 100 is made of transparent polymer material, so as to achieve the effect of conveniently observing the inside of the dish body 100. It should also be noted that, as those skilled in the art will appreciate, the specific shape of the body region 110 is not limited by the present utility model, and the shape of the cross section (the cross section perpendicular to the height direction) of the body region 110 includes, but is not limited to, a circle, a rectangle, a square, an ellipse, and the like.

Specifically, the volume of the washing chamber 130 is 25. Mu.L to 100. Mu.L, the volume of the culturing chamber 120 is more than 23. Mu.L, and the culturing chamber 120 can hold 23. Mu.L to 33. Mu.L of the culture solution so that a single embryo enjoys 23. Mu.L to 33. Mu.L of the culture solution. In the actual use process, adding flushing liquid into the flushing bin 130, adding culture solution into the culture bin 120, and adding 23-33 mu L of culture solution into the culture bin 120 by using a pipetting gun; after the liquid is balanced for a period of time, sucking the embryo by using a micro-tube, and rinsing in a rinsing bin 130 to remove cell debris; the embryos are then transferred to culture wells 121 in the culture bins 120 for culturing, and finally a disposable pipette is used to add culture oil into the culture bins 120, the level of which should not exceed the top of the barrier structure.

Further, the diameter of the inner cavity of the culture bin 120 is 3.2 mm-3.6 mm, and the depth of the inner cavity of the culture bin 120 is 2.35 mm-2.65 mm. Thus, such sizing ensures that the culture compartment 120 can hold 23. Mu.L to 33. Mu.L of culture solution.

Further, the outer diameter of the annular boss 140 is 3.5 mm-3.8 mm, and the height of the annular boss 140 is 0.3 mm-0.5 mm. Thus, the annular boss 140 can have a certain wall thickness and a certain height, so that the annular boss 140 can smoothly block the coverage area of each culture bin 120.

In an exemplary embodiment, inside the flushing bin 130, the inner circumferential dimension of the flushing bin 130 is gradually reduced from top to bottom, and the inner wall of the flushing bin 130 is inclined at an angle of 10 ° to 60 ° with respect to the vertical direction. Therefore, by arranging the flushing bin 130 in a gathering structure, an operator can conveniently and smoothly flow flushing liquid when using the disposable suction tube to add the flushing liquid, and the generation of bubbles is reduced. It should be noted that, as those skilled in the art will appreciate, the specific shape of the flushing cartridge 130 is not limited by the present utility model, and the cross-sectional shape (cross-section perpendicular to the height direction) of the flushing cartridge 130 includes, but is not limited to, circular, rectangular, square, slot-shaped, etc.

With continued reference to fig. 1 and 4, in an exemplary embodiment, the plurality of flushing bins 130 are disposed on either side of the plurality of culture bins 120, as shown in fig. 1 and 4. Therefore, by arranging the plurality of flushing bins 130 on both sides of the plurality of culturing bins 120, it is not only convenient for an operator to perform culturing in the culturing holes 121 in the neighboring culturing bins 120 after rinsing the embryo in the flushing bins 130, but also more convenient for the operator to rapidly perform positioning of the culturing bins 120 according to the positions of the flushing bins 130.

With continued reference to fig. 6, as shown in fig. 6, the culture hole 121 is recessed downward with respect to the inner bottom wall of the culture bin 120, and the inner diameter of the culture hole 121 gradually decreases from top to bottom. Thus, by this arrangement, it is possible to effectively ensure that the nutrient solution in the culture bin 120 can slowly and smoothly flow into the culture holes 121, and effectively reduce the generation of bubbles.

In an exemplary embodiment, the maximum diameter of the culture well 121 is 1.05mm to 1.08mm, the minimum diameter of the culture well 121 is 0.2mm to 0.25mm, and the depth of the culture well 121 is 0.50mm to 0.55mm. Thus, this arrangement ensures that the minimum diameter of the culture well 121 is greater than the diameter of the embryo (the diameter of the embryo is about 0.1 mm), thereby ensuring that the culture well 121 provides sufficient space for the embryo, while ensuring that the maximum diameter of the culture well 121 is greater than the diameter of the pipette tip (the diameter of the pipette tip is about 0.9 mm), while ensuring that the culture well 121 has sufficient depth to effectively prevent the embryo from being released from the culture well 121.

In an exemplary embodiment, the distance between the center lines of two adjacent culture bins 120 is 6.0mm to 6.5mm. Thus, the distance between the culture chambers 120 can be ensured to be independent of each other, so that the occurrence of liquid strings of liquid such as culture liquid and culture oil between the culture chambers 120 can be prevented, and the number of the culture chambers 120 which can be arranged in the dish body 100 can be prevented from being influenced by the overlarge distance between the adjacent culture chambers 120.

With continued reference to fig. 1, 6 and 7, as shown in fig. 1, 6 and 7, the main body area 110 is in a gathered shape from top to bottom, specifically, the main body area 110 includes a surrounding block 111, a gathering slope 112 and a concave table 113, which are sequentially connected from top to bottom, the culture bin 120 and the flushing bin 130 are located in an area surrounded by the concave table 113, the culture bin 120 is concavely arranged downward relative to the concave table 113 to extend to the lower side of the main body area 110, and the flushing bin 130 is convexly arranged upward relative to the concave table 113 to extend to the area surrounded by the gathering slope 112. Thus, by providing the accommodating portion of the three-stage structure, firstly, when liquid (including flushing liquid, culture liquid or culture oil) is added, the liquid can slowly flow to the bottom of the flushing bin 130 or the culture bin 120 along the gathering slope 112, so that turbulence is prevented from being generated when the liquid is added, and a large amount of bubbles are generated; secondly, when the culture oil is overloaded, the culture dish is effectively prevented from shaking violently in the time difference incubator, and a large amount of bubbles are generated due to turbulence of the culture solution and the culture oil in the culture dish; when shaking occurs, the culture oil can flow back along the gathering slope 112 without generating bubbles; thirdly, the accommodating part provides a gas exchange space, the time difference incubator provides oxygen favorable for embryo development, the embryo growth generates carbon dioxide gas, and the oxygen or the carbon dioxide gas can enter and exit the accommodating part through the culture oil layer; finally, the shape of the accommodating part is matched with the shape of the incubator, so that the culture dish is conveniently fixed in the incubator.

Further, as shown in fig. 6 and 7, the inner peripheral dimension of the gathering slope 112 is gradually reduced from top to bottom, and the inner wall of the gathering slope 112 is inclined at an angle of 5 ° to 45 ° with respect to the horizontal direction. Thus, by providing a relatively large angle slope in the gathering slope 112, both left-handed and right-handed operators can load embryos and liquids (including culture liquid and culture oil) to successfully perform the relevant operations.

With continued reference to fig. 6 and 7, as shown in fig. 6 and 7, the inner peripheral dimension of the enclosure 111 gradually decreases from top to bottom, and the inner wall of the enclosure 111 is inclined at an angle greater than 0 ° and less than or equal to 30 ° with respect to the vertical direction. Therefore, by arranging the slope with a smaller angle in the enclosure 111, the culture solution and the culture oil can be properly contained, and a large number of bubbles caused by turbulent flow of the culture solution and the culture oil due to severe shaking of the embryo culture dish when the embryo culture dish is matched with the base of the time difference incubator can be further effectively prevented; at the same time, sufficient space is reserved for gas exchange. It should be noted that, in other embodiments, the enclosure 111 may be a straight cavity structure, that is, the enclosure 111 may have an angle of 0 ° with respect to the vertical direction, as will be appreciated by those skilled in the art.

Further, the concave table 113 includes an inner wall in a vertical direction and an inner wall in a horizontal direction, which are disposed at an angle of 90 ° to 110 °, and the depth of the concave table 113 is 1mm to 2mm. Therefore, the independent embryo culture dish provided by the utility model can be further effectively prevented from shaking violently when being matched with the base of the time difference incubator, a large number of bubbles generated due to turbulence of culture solution and culture oil are reduced, and the independent embryo culture dish provided by the utility model can be more conveniently matched with the base of the time difference incubator.

With continued reference to fig. 1 and 4, the outer sidewalls of the flushing cartridge 130 on the same side are connected to each other as shown in fig. 1 and 4. Thus, the arrangement ensures the structural stability of the flushing cartridge 130, so that the flushing cartridge 130 can be firmly fixed in the body region 110.

With continued reference to fig. 1 to 3, as shown in fig. 1 to 3, a handle 150 is disposed at the top of the dish body 100, the handle 150 is located outside the main body 110, and the handle 150 protrudes upward relative to the main body 110. Therefore, by arranging the handles 150, an operator can conveniently pinch the embryo culture dish by one hand through the two handles 150, so that the pollution to the culture solution and embryos in the dish body 100 caused by frequent direct contact of hands with the dish body 100 is avoided, and meanwhile, the contact area of a pinching area can be increased. It should be noted that, as those skilled in the art will understand, the number of the handles 150 may be set according to the specific situation, and the present utility model is not limited thereto, and for example, the number of the handles 150 may be 1 or 2.

In an exemplary embodiment, the upper surface of the handle 150 is provided in a circular arc shape. Thus, this arrangement is more thumb-like and more convenient for an operator to hold the handle 150 in one hand.

Further, a first sanding layer is provided on the outer side of the handle 150. This arrangement thus increases friction, further facilitating one-hand grasping of the handle 150 by the operator. It should be noted that, as will be understood by those skilled in the art, the first sanding layer may be formed by roughening or sandblasting the surface of the handle 150.

With continued reference to fig. 1 and 4, as shown in fig. 1 and 4, the top of the dish body 100 is further provided with a label attaching area 160, and the label attaching area 160 is located outside the main body area 110. Therefore, a label carrying two-dimensional code or bar code of relevant information of the patient can be stuck on the label sticking area 160, so that the tracking and management of embryos can be more convenient for operators. It should be noted that, as those skilled in the art will appreciate, the specific shape of the label application area 160 is not limited by the present utility model, and the shape of the label application area 160 includes, but is not limited to, an arch, a rectangle, and a trapezoid.

With continued reference to fig. 1, as shown in fig. 1, the label applying area 160 is disposed at an angle of 10 ° to 45 ° with respect to the horizontal direction. Therefore, by obliquely arranging the mark pasting region 160, the label pasted on the mark pasting region 160 can be completely recognized by the recognition system in the time difference incubator when the independent embryo culture dish provided by the utility model is matched with the time difference incubator.

In one exemplary embodiment, the label application area 160 is provided with a second abrasive layer. Thus, this arrangement ensures that the marker can be inked, facilitating the operator to place a mark through the marker in the label application area 160. It should be noted that the second sanding layer may be formed by roughening or sand blasting the surface of the label application area 160, as will be appreciated by those skilled in the art.

Further, as shown in fig. 1, two handles 150 are provided on the top of the dish body 100, and the label attaching area 160 is provided between the two handles 150. Thus, the arrangement not only saves the internal space of the culture dish, but also further ensures that the label attached to the label attachment area 160 can be completely recognized by the recognition system in the time difference incubator.

With continued reference to fig. 2 and 5, as shown in fig. 2 and 5, a first identifier 310 for identifying the culture bins 120 is provided at the bottom of the culture bins 120, where each culture bin 120 corresponds to one first identifier 310. Since the photographing area is located right below the culture hole 121 in the culture chamber 120, the first identifier 310 should not overlap or intersect with the area where the culture hole 121 is located on the projection plane, i.e. the first identifier 310 is disposed below the culture chamber 120 and is offset from the center line of the culture hole 121 in the culture chamber 120. Thus, by setting the first identifier 310, it may be convenient to quickly distinguish embryos in different culture bins 120. It should be noted that, as will be understood by those skilled in the art, the first identifier 310 may be disposed on the inner side of the culture compartment 120 or may be disposed on the outer side of the culture compartment 120, and is preferably disposed on the inner side of the culture compartment 120.

Further, as shown in fig. 2 and 5, a second identifier 320 for identifying the flushing bin 130 is provided below the flushing bin 130, where each flushing bin 130 corresponds to one second identifier 320. By setting the second identifier 320, a quick differentiation between different flushing cartridges 130 can be facilitated. It should be noted that, as will be understood by those skilled in the art, the second identifier 320 may be disposed on the inner side of the flushing cartridge 130, or may be disposed on the outer side of the flushing cartridge 130 (i.e., the second identifier 320 is disposed in the bottom view direction below the flushing cartridge 130), where it is preferably disposed on the outer side of the flushing cartridge 130. It should also be noted that, as shown in fig. 2 and 5, since the flushing cartridge 130 is provided to extend upward, it is preferable that the second identifier 320 is provided at a region corresponding to the flushing cartridge 130 on the outer bottom wall of the main body region 110.

It should be noted that, as those skilled in the art will appreciate, the specific types of the first identifier 310 and the second identifier 320 are not limited by the present utility model, the types of the first identifier 310 and the second identifier 320 include, but are not limited to, numerals, english letters, roman characters, etc., the alignment of the first identifier 310 and the second identifier 320 is not limited by the present utility model, each of the first identifier 310 may be aligned centrally, aligned upwardly or aligned downwardly, and each of the second identifiers 320 may be aligned centrally, aligned upwardly or aligned downwardly.

With continued reference to fig. 2 and 6, as shown in fig. 2 and 6, the outer bottom wall of the culturing chamber 120 is provided with a supporting table 170 extending downward, and the bottom of the supporting table 170 is provided with the first identifier 310 for identifying the culturing chamber 120. The boss has the functions of: 1) The independent embryo culture dish can be stably fixed on the base, namely limited by being matched with the base of the time difference incubator; 2) Maintaining the spacing between the bottom of the support table 170 and the bottom of the culture well 121 facilitates zooming of the incubator lens.

Example two

Referring to fig. 8 to 14, fig. 8 schematically shows a schematic perspective view of a vessel body of a free-standing embryo culture vessel according to a second embodiment of the utility model at a main viewing angle; FIG. 9 schematically illustrates a perspective view of the dish of FIG. 8 at a bottom view; FIG. 10 schematically illustrates a front view of the dish of FIG. 8; FIG. 11 schematically shows a top view of the dish of FIG. 8; FIG. 12 schematically illustrates a bottom view of the dish of FIG. 8; FIG. 13 schematically shows a cross-sectional view of the bowl of FIG. 8 along the C-C plane; fig. 14 schematically shows a cross-sectional view of the dish of fig. 8 along the D-D plane.

As shown in fig. 8 to 14, the dish body 100 of the independent embryo culture dish provided in the present embodiment is different from the dish body 100 of the independent embryo culture dish provided in the first embodiment in that: the barrier structures vary. As shown in fig. 8 and 11, in the present embodiment, a grating 180 is provided in the main body region 110, and the grating 180 is located above the culture bins 120 and disposed around the culture bins 120, so that a separate culture oil coverage area is formed above each culture bin 120. The grating 180 includes a plurality of parallel transverse grating bars 181 and a plurality of parallel longitudinal grating bars 182, and the transverse grating bars 181 and the longitudinal grating bars 182 intersect each other to form a blocking structure above each of the culture bins 120, which is disposed around the culture bins 120, that is, the blocking structure above each of the culture bins 120 is formed by the grating 180. Therefore, by using the grating 180 to separate the coverage areas of the culture bins 120, the whole structure of the independent embryo culture dish provided by the utility model can be effectively simplified, and the processing procedure of the independent embryo culture dish provided by the utility model can be simplified. It should be noted that, as those skilled in the art will appreciate, the grille 180 and the main body 110 may be configured as a single unit or may be configured as a split unit, which is not limited by the present utility model.

Specifically, as shown in fig. 8 and 11, the transverse grid bars 181 extend along the length direction of the dish body 100, the longitudinal grid bars 182 extend along the width direction of the dish body 100, the plurality of flushing chambers 130 are respectively disposed on two sides of the plurality of culturing chambers 120 along the length direction of the dish body 100, two ends of the transverse grid bars 181 are respectively abutted against corresponding outer side walls of the flushing chambers 130, and two ends of the longitudinal grid bars 182 are respectively abutted against two side inner walls of the main body area 110 (specifically, two side inner walls of the recess 113 in the vertical direction). Thus, this arrangement further facilitates the formation of a barrier structure over each of the culture compartments 120 that is disposed around the culture compartments 120.

Further, as shown in fig. 8 and 11, small holes 183 are provided at the connection points of the transverse grating bars 181 and the longitudinal grating bars 182. Therefore, when the culture oil in one of the culture bins 120 overflows the barrier structure corresponding to the culture bin 120, the overflowed culture oil will flow into the small holes 183 on the grid 180 first, but not directly flow into the other culture bins 120, so as to effectively prevent the occurrence of fluid mixing between the culture bins 120. Meanwhile, the small holes 183 can also play a role in positioning, so that an operator can conveniently and quickly position the culture bin 120 according to the positions of the small holes 183. It should be noted that, as those skilled in the art will appreciate, the small holes 183 may extend through the entire thickness of the grille 180 (i.e., the small holes 183 are in a through hole structure), or may extend through only a portion of the thickness of the grille 180 (i.e., the small holes are in a groove structure), which is not limited by the present utility model.

In an exemplary embodiment, the height of the grating 180 is 0.3mm to 0.5mm. Thus, such a size arrangement ensures that the grille 180 can smoothly function as a barrier to the coverage area of each of the culture compartments 120.

Example III

Referring to fig. 15 to 17, fig. 15 schematically illustrates a front view of a cover of a free-standing embryo culture dish according to an embodiment of the present utility model; FIG. 16 schematically illustrates a cross-sectional view of the capsule of FIG. 15; fig. 17 schematically shows a top view of the capsule shown in fig. 15. As shown in fig. 15 to 17, the independent embryo culture dish provided by the present utility model further comprises a dish cover 200 for covering the main body area 110 in a use state. Thus, by providing the dish cover 200, the contamination of the culture solution and embryos in the dish body 100 can be effectively prevented.

Preferably, a gap is provided between the capsule 200 and the body region 110. Because a gap is provided between the capsule 200 and the main body 110, it is ensured that the culture chamber 120 and the outside can maintain gas flow exchange, so that the embryos in the culture chamber 120 can obtain the gas required in the culture process. It should be noted that, as will be appreciated by those skilled in the art, the capsule 200 shown in fig. 15 to 17 may be used with the capsule 100 of the above embodiments, and the capsule 200 is made of a transparent polymer material, so as to facilitate the observation of the embryo in the capsule 100. It should also be noted that the specific shape of the capsule 200 is not limited by the present utility model, and the shape of the capsule 200 includes, but is not limited to, rounded rectangle, square, or circle, as will be appreciated by those skilled in the art.

With continued reference to fig. 16, as shown in fig. 16, further, a limit stop 210 is provided on the inner surface of the top end of the capsule 200. As will be appreciated by those skilled in the art, the term "top" refers to the top of the capsule 200 in the use configuration. Thus, the limit stop 210 ensures that the capsule 200 can leave a vertical gap when being matched with the capsule body 100, so as to ensure gas flow exchange. It should be noted that, as those skilled in the art will appreciate, the specific shape of the limit stop 210 is not limited by the present utility model, and the shape of the limit stop 210 includes, but is not limited to, a half crescent, a circle, or a square. It should be further noted that, as those skilled in the art will appreciate, the specific location of the limit stop 210 on the inner surface of the top end of the capsule 200 is not limited in the present utility model, and the specific location of the limit stop 210 includes, but is not limited to, one or more of four corners of the inner surface of the top end of the capsule 200. Meanwhile, as will be appreciated by those skilled in the art, the number of the limit stops 210 is not limited in the present utility model, and may be set according to practical situations, for example, the number of the limit stops 210 may be 1, 2, 3 or more.

Preferably, a left-right and front-back gap is left between the inner contour of the dish cover 200 and the outer contour of the main body 110 of the dish body 100100. This arrangement can thereby further ensure gas flow exchange.

With continued reference to fig. 15 to 17, as shown in fig. 15 to 17, further, an anti-collision block 220 is provided on the outer surface of the top end of the capsule 200. Therefore, the anti-collision block 220 can avoid the problem that the upper surface of the dish cover 200 (namely, the outer surface of the top end of the dish cover 200) is scratched by an operator when the dish cover 200 is placed, so that the image observation of the embryo in the time difference incubator is affected. It should be noted that, as those skilled in the art will appreciate, the specific shape of the bump stop 220 is not limited by the present utility model, and the shape of the bump stop 220 includes, but is not limited to, a half crescent, a circle or a square. Furthermore, as those skilled in the art will appreciate, the present utility model is not limited to the specific location of the bump stop 220 on the outer surface of the top end of the dish cover 200, and the specific location of the bump stop 220 includes, but is not limited to, one or more of four corners of the outer surface of the top end of the dish cover 200. Meanwhile, as those skilled in the art will appreciate, the present utility model is not limited to the specific number of the bump stop blocks 220, and may be specifically set according to practical situations, for example, the number of the bump stop blocks 220 may be 1, 2, 3 or more.

With continued reference to fig. 15 and 17, as shown in fig. 15 and 17, the outer peripheral sidewall of the capsule 200 is provided with a slip prevention member 230. Therefore, the friction between the hand of the operator and the capsule 200 can be increased by the anti-slip member 230, so that the capsule 200 can be effectively prevented from slipping off the hand of the operator. It should be noted that, as those skilled in the art will appreciate, the specific structure of the anti-skid member 230 is not limited in the present utility model, for example, the anti-skid member 230 may be an anti-skid groove provided on the dish cover 200, and the anti-skid member 230 may also be an anti-skid stripe provided on the dish cover 200.

In summary, compared with the prior art, the independent embryo culture dish provided by the utility model has the following advantages:

the independent embryo culture dish provided by the utility model comprises a dish body 100, wherein the dish body 100 comprises a main body area 110 and a plurality of culture bins 120 arranged in the main body area 110, and any adjacent culture bins 120 are arranged at intervals; the bottom of each culturing chamber 120 is provided with a culturing hole 121 for placing embryos, and a blocking structure arranged around the culturing chamber 120 is arranged above each culturing chamber 120, so that each culturing chamber 120 has an independent coverage area. Therefore, by the blocking structure, each culture bin 120 has an independent coverage area, so that the culture oil in each culture bin 120 is prevented from being mixed with each other, the independence of each culture bin 120 is ensured, the function of marking the loading position of the culture oil can be achieved in the process of adding the culture oil, the loading operation of the culture oil is facilitated, and the overload of the loading amount of the culture oil is effectively prevented.

In addition, the present utility model can be used for rinsing the embryo before culturing to remove cell fragments and harmful substances by providing the rinsing bin 130 in the main body region 110, thereby further improving the molding rate of the blastocyst.

The above description is only illustrative of the preferred embodiments of the present utility model and is not intended to limit the scope of the present utility model, and any alterations and modifications made by those skilled in the art based on the above disclosure shall fall within the scope of the present utility model. It will be apparent to those skilled in the art that various modifications and variations can be made to the present utility model without departing from the spirit or scope of the utility model. Thus, the present utility model is intended to include such modifications and alterations insofar as they come within the scope of the utility model or the equivalents thereof.

Claims (15)

1. The independent embryo culture dish is characterized by comprising a dish body, wherein the dish body comprises a main body area and a plurality of culture bins arranged in the main body area, and any adjacent culture bins are arranged at intervals;

the bottom of each cultivate the storehouse all is equipped with one and is used for placing the culture hole of embryo, and each cultivate the top of storehouse all be equipped with around this separation structure of cultivateing the storehouse setting, so that each cultivate the storehouse all has independent coverage area.

2. The free-standing embryo culture dish of claim 1, wherein the barrier structure above each of the culture compartments is an annular boss disposed around the culture compartment, the outer diameter of the annular boss is greater than the inner diameter of the culture compartment, the outer diameter of the annular boss is 3.5 mm-3.8 mm, and the height of the annular boss is 0.3 mm-0.5 mm.

3. A freestanding embryo culture dish as claimed in claim 2, wherein in the main body area there are provided, in addition to the area in which the culture compartment is located, flushing compartment areas on one or both opposite sides of the area in which the culture compartment is located, each flushing compartment area being provided with a plurality of flushing compartments spaced apart from each other.

4. The free standing embryo culture dish of claim 1, wherein the barrier structure above each of the culture compartments is constituted by a grating disposed within the body area, the grating disposed about the culture compartments, the grating comprising a plurality of parallel disposed transverse grating strips and a plurality of parallel disposed longitudinal grating strips, the transverse grating strips and the longitudinal grating strips intersecting one another.

5. The free standing embryo culture dish of claim 4 wherein the junction of the transverse and longitudinal grating strips is provided with apertures.

6. The free-standing embryo culture dish of claim 4, wherein in the body area, in addition to the area in which the culture compartment is located, there are provided flushing compartment areas on one side or on opposite sides of the area in which the culture compartment is located, each flushing compartment area being provided with a plurality of flushing compartments spaced apart from each other.

7. The free-standing embryo culture dish according to claim 6, wherein the plurality of flushing chambers are respectively arranged on two sides of the plurality of culture chambers along the length direction of the dish body, two ends of the transverse grid bars are respectively abutted with the outer side walls of the corresponding flushing chambers, and two ends of the longitudinal grid bars are respectively abutted with the inner walls of two sides of the main body area.

8. The free-standing embryo culture dish of claim 3 or 6, wherein the main body area comprises a surrounding baffle, a gathering slope and a concave table which are sequentially connected from top to bottom, the culture bin is positioned in an area surrounded by the concave table, the culture bin is arranged in a downward concave manner relative to the concave table so as to extend to the lower part of the main body area, and the flushing bin is arranged in an upward convex manner relative to the concave table so as to extend to the area surrounded by the gathering slope.

9. The free-standing embryo culture dish of claim 8 wherein the body area comprises one or more of the following features:

the inner peripheral dimension of the gathering slope is gradually reduced from top to bottom, and the inner wall of the gathering slope is obliquely arranged at an angle of 5-45 degrees with the horizontal direction;

the inner peripheral dimension of the enclosure is gradually reduced from top to bottom, and the inner wall of the enclosure is obliquely arranged at an angle of more than 0 degrees and less than or equal to 30 degrees with the vertical direction; and

the concave table comprises an inner wall in the vertical direction and an inner wall in the horizontal direction, wherein the inner wall is arranged at an angle of 90-110 degrees, and the depth of the concave table is 1-2 mm.

10. The free-standing embryo culture dish of claim 1, wherein the outer bottom wall of the culture compartment is provided with a downwardly extending support platform, and the bottom of the support platform is provided with a first identifier for identifying the culture compartment.

11. The free-standing embryo culture dish of claim 1 wherein the dish body comprises one or more of the following features:

the distance between the central lines of two adjacent culture bins is 6.0 mm-6.5 mm;

the top of the dish body is provided with a handle, the handle is positioned outside the main body area, the handle is upwards arranged in a protruding mode relative to the main body area, the upper surface of the handle is arranged in a circular arc shape, and/or a first sand grinding layer is arranged on the outer side of the handle;

The top of the dish body is also provided with a mark pasting area, the mark pasting area is positioned outside the main body area, the mark pasting area is obliquely arranged at an angle of 10-45 degrees with the horizontal direction, and the mark pasting area is provided with a second sand grinding layer.

12. The free-standing embryo culture dish of claim 3 or 6 wherein the rinsing cartridge comprises one or more of the following features:

the inner peripheral dimension of the flushing bin is gradually reduced from top to bottom, and the inner wall of the flushing bin is obliquely arranged at an angle of 10-60 degrees with the vertical direction;

and a second identifier for identifying the flushing bin is arranged on the lower bottom view direction of the flushing bin.

13. The free-standing embryo culture dish according to claim 1, wherein the inner diameter of the culture well gradually decreases from top to bottom, the maximum diameter of the culture well is 1.05mm to 1.08mm, the minimum diameter of the culture well is 0.2mm to 0.25mm, and the depth of the culture well is 0.50mm to 0.55mm.

14. The free-standing embryo culture dish of claim 1, wherein the diameter of the inner cavity of the culture chamber is 3.2 mm-3.6 mm, and the depth of the inner cavity of the culture chamber is 2.35 mm-2.65 mm.

15. A freestanding embryo culture dish as claimed in claim 1, further comprising a dish cover for covering the body area in use, the dish cover having one or more of the following features:

a gap is arranged between the dish cover and the main body area;

an anti-collision stop block is arranged on the outer surface of the top end of the dish cover;

the inner surface of the top end of the dish cover is provided with a limit stop;

and an anti-slip piece is arranged on the peripheral side wall of the dish cover.