CN112834763A

CN112834763A - Detection chip and detection system

Info

Publication number: CN112834763A
Application number: CN202010367824.8A
Authority: CN
Inventors: 赵静; 张玙璠; 袁春根; 安光明
Original assignee: BOE Technology Group Co Ltd
Current assignee: BOE Technology Group Co Ltd
Priority date: 2019-11-22
Filing date: 2020-04-30
Publication date: 2021-05-25
Also published as: CN211905402U; US20220241774A1; EP4067907A1; JP2023522804A; WO2021218537A1; EP4067907A4; KR20230005803A

Abstract

A detection chip and a detection system are provided, wherein the detection chip comprises a sample adding opening and at least one detection branch structure. Each of the at least one detection branch structures includes a detection section; the detection part comprises a detection groove and a reaction reagent, the detection groove is communicated with the sample adding opening, the reaction reagent is contained in the detection groove, and the detection part is configured to allow the optical detection of the reaction reagent in the detection groove. The detection chip can help to realize automatic detection and can help to realize a detection device which is convenient to carry.

Description

Detection chip and detection system

This application claims priority from chinese patent application No. 201911159478.8, filed on 22/11/2019, which is incorporated by reference in its entirety as part of this application.

Technical Field

The embodiment of the disclosure relates to a detection chip and a detection system.

Background

The micro-fluidic chip technology integrates basic operation units related to sample preparation, reaction, separation, detection and the like in the fields of biology, chemistry, medicine and the like into a chip with a micro-channel with a micron scale, and automatically completes the whole process of reaction and analysis. The chip used in this process is called a microfluidic chip, and may also be called a Lab-on-a-chip (Lab-on-a-chip). The microfluidic chip technology has the advantages of small sample consumption, high analysis speed, convenience for manufacturing a portable instrument, suitability for real-time and on-site analysis and the like, and is widely applied to various fields of biology, chemistry, medicine and the like.

Disclosure of Invention

At least one embodiment of the present disclosure provides a detection chip, which includes a sample addition opening and at least one detection branch structure; each of the at least one detection branch structure includes a detection portion including a detection groove and a reaction reagent, wherein the detection groove communicates with the sample addition opening, the reaction reagent is accommodated in the detection groove, and the detection portion is configured to allow optical detection of the reaction reagent located in the detection groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, each of the at least one detection branch structures further includes a flow guide groove, where the flow guide groove has a first end and a second end, the first end of the flow guide groove is communicated with the sample adding opening, and the second end of the flow guide groove is communicated with the detection groove, so that the detection groove is communicated with the sample adding opening through the flow guide groove.

For example, at least one embodiment of the present disclosure provides a detection chip further including: the sample feeding opening is a through hole in the first substrate, and the diversion trench and the detection groove are formed on the first surface of the first substrate; a second substrate laminated on the first surface of the first substrate and allowing the optical detection at a position corresponding to the detection groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, each of the at least one detection branch structures further includes a water absorption film, which is accommodated in the detection groove and at least partially overlaps with the reaction reagent in a direction perpendicular to the first substrate.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the water absorption film is disposed on a side of the reaction reagent away from the second substrate.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the liquid storage amount of the water absorption film is 10 μ L to 50 μ L.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the detection portion further includes a flow guide groove located around the detection groove, and the flow guide groove is communicated with the detection groove; at least part of the diversion grooves are smaller than the detection grooves in height.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the flow guide groove includes a flow guide wall in a slope shape, and one end of the flow guide wall is connected to a side surface of the detection groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the flow guide groove and the longitudinal section of the detection groove are integrally stepped.

For example, in a detection chip provided in at least one embodiment of the present disclosure, the flow guide groove at least partially surrounds the detection groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the height of the detection groove is 0.2mm to 5mm, the difference between the maximum height of the flow guide groove and the height of the detection groove is 0.1mm to 1mm, and the width of the flow guide groove is 0.1mm to 1 mm.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the detection portion further includes a liquid storage through hole penetrating through the first substrate and communicating with the detection groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the liquid storage through hole communicates with the center of the detection groove.

For example, in the detection chip provided by at least one embodiment of the present disclosure, the diameter of the liquid storage through hole is 0.2mm to 5mm, and the ratio of the depth of the liquid storage through hole to the height of the detection groove is 0.5:1 to 10: 1.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the height of the flow guide groove is 0.1mm to 1.5mm, and the width of the flow guide groove is 0.1mm to 2 mm.

For example, in the detection chip provided in at least one embodiment of the present disclosure, a ratio of the height of the flow guide groove to the width of the flow guide groove is 1:1-10: 1.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the inner wall of the flow guide groove has hydrophilicity.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the at least one detection branch structure includes a plurality of detection branch structures, and the plurality of detection branch structures are uniformly distributed along the periphery of the sample addition opening.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample addition opening includes a first main body and a first protrusion protruding from the first main body to the flow guide groove, and the first protrusion is communicated with the flow guide groove.

For example, in at least one embodiment of the present disclosure, there is provided a detection chip, wherein the first body has a diameter of 1mm to 10 mm.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction reagent includes a reaction membrane and/or a bulk reaction reagent.

For example, in a detection chip provided in at least one embodiment of the present disclosure, the reaction membrane is in a compressed state in a thickness direction.

For example, in the detection chip provided in at least one embodiment of the present disclosure, a ratio of a thickness of the reaction film in a relaxed state to a height of the detection groove is 1:1 to 1: 0.5.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction film is circular, or the reaction film is polygonal, and one corner of the polygon is directly communicated with the second end of the flow guide groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction membrane includes a membrane main body and a second protrusion protruding from the membrane main body to the flow guide groove, and at least a part of the second protrusion is located in the flow guide groove; the membrane main body is in the same shape as the detection groove and is circular.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction membrane has a diamond shape.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the detection groove is 3mm to 15mm, and the diameter of the reaction membrane is equal to or smaller than the diameter of the detection groove.

For example, in a detection chip provided in at least one embodiment of the present disclosure, the second substrate has a detection through hole at a position corresponding to the detection groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the second substrate is opaque to light; or, the detection chip further comprises a light shielding layer, wherein the light shielding layer covers the surface of the second substrate far away from and/or close to the first substrate, and exposes the detection through hole.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the diameter of the detection through hole is 2mm to 10 mm.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the material of the first substrate includes one or more of polymethyl methacrylate, polystyrene, and polycarbonate.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the material of the second substrate includes one or more of polymethyl methacrylate, polystyrene, polycarbonate, and polyethylene terephthalate.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the first substrate and the second substrate are bonded, welded, adhered, or snapped.

For example, at least one embodiment of the present disclosure provides a detection chip further comprising an adhesive layer; wherein the adhesive layer is positioned between the first substrate and the second substrate and used to bond the first substrate and the second substrate, the adhesive layer including an opening corresponding to the detection groove.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the reaction reagent includes a matrix material including glass fiber, cotton fiber, or a composite fiber of glass fiber and cotton fiber, and a detection reagent distributed in the matrix material.

For example, at least one embodiment of the present disclosure provides a detection chip further including: the sample feeding opening is a through hole in the first substrate, and the diversion trench and the detection groove are formed on the first surface of the first substrate; a second substrate laminated on the first surface of the first substrate and having a detection through-hole at a position corresponding to the detection groove to allow the optical detection through the detection through-hole; the at least one detection branch structure comprises a plurality of detection branch structures which are uniformly distributed along the periphery of the sample adding opening.

For example, at least one embodiment of the present disclosure provides a detection chip further including: the sample feeding opening is a through hole in the first substrate, the detection groove is formed on the first surface of the first substrate, and the flow guide groove is formed on the second surface of the first substrate; a second substrate that is laminated on the first surface of the first substrate and allows the optical detection at a position corresponding to the detection groove; and a third substrate stacked on the second surface of the first substrate and sealing the sample injection opening and the guide groove.

For example, at least one embodiment of the present disclosure provides a detection chip further including: the sample feeding opening, the flow guide groove and the detection groove are formed on the first surface of the first substrate, and the sample feeding opening is a non-through hole in the first substrate; a second substrate laminated on the first surface of the first substrate and exposing the sample addition opening and allowing the optical detection at a position corresponding to the detection groove.

For example, at least one embodiment of the present disclosure provides a detection chip further comprising an optical calibration branching structure, wherein the optical calibration branching structure comprises an optical path detection area configured to perform optical calibration.

For example, the detection chip provided in at least one embodiment of the present disclosure further includes a sample addition protrusion: the sample adding bulge protrudes from the first surface of the first substrate along the direction far away from the second substrate, and one end of the sample adding bulge is connected with the sample adding opening.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the sample addition protrusion includes a conical cavity, a volume of the conical cavity is 50 μ L to 200 μ L, and a height of the conical cavity protruding from the first substrate is 1mm to 20 mm; the conical cavity comprises a first end and a second end which are opposite to each other, the first end is connected with the sample adding opening, the diameter of the first end is 0.5mm-5mm, and the diameter of the second end is 1mm-20 mm.

For example, in the detection chip provided in at least one embodiment of the present disclosure, the first substrate includes a first notch, the second substrate includes a second notch corresponding to the first notch, and the first notch and the second notch are used to fix the detection chip.

At least one embodiment of the present disclosure also provides a detection system, including: the detection device and the detection chip of any embodiment of the disclosure; the detection device is configured to detect the reaction reagent in the detection groove through the detection portion.

For example, in a detection system provided in at least one embodiment of the present disclosure, the detection device includes: a light source configured to emit light toward the reaction reagent; a photodetection device configured to receive light emitted from the light source and reflected by the reactive agent.

Drawings

To more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below, and it is apparent that the drawings in the following description relate only to some embodiments of the present disclosure and are not limiting to the present disclosure.

Fig. 1A and 1B are a front perspective view and a back perspective view of a detection chip according to at least one embodiment of the present disclosure, respectively;

FIGS. 2A and 2B are exploded views of the detection chip shown in FIGS. 1A and 1B, respectively;

FIGS. 3A and 3B are front and back perspective views, respectively, of a first substrate of the detection chip shown in FIGS. 1A and 1B;

FIG. 4 is a schematic plan view of a first surface of a first substrate of the detection chip shown in FIG. 1A and FIG. 1B;

FIG. 5 is a schematic plan view of a second substrate of the detection chip shown in FIG. 1A and FIG. 1B;

FIG. 6 is a schematic view showing the structure of a reaction membrane of the detection chip shown in FIGS. 1A and 1B;

FIGS. 7A and 7B are a front perspective view and a back perspective view, respectively, of another detection chip provided in at least one embodiment of the present disclosure;

FIG. 8 is an exploded view of the sense die shown in FIGS. 7A and 7B;

FIGS. 9A and 9B are front and back perspective views, respectively, of the first substrate of the detection chip shown in FIGS. 7A and 7B;

FIG. 10 is a schematic plan view of the first surface of the first substrate of the detecting chip shown in FIG. 7A and FIG. 7B;

fig. 11 is an exploded view of another detection chip according to at least one embodiment of the present disclosure;

FIG. 12 is a schematic structural diagram of a first substrate of the detection chip shown in FIG. 11;

FIG. 13A is a schematic diagram of a portion of the first substrate of the detecting chip shown in FIG. 12;

FIG. 13B is a schematic cross-sectional view taken along line A-A' of FIG. 13A;

FIG. 13C is a schematic cross-sectional view taken along line B-B' of FIG. 13A;

fig. 14 is an exploded view of another detection chip according to at least one embodiment of the present disclosure;

fig. 15A and 15B are exploded views of still another detection chip according to at least one embodiment of the present disclosure;

fig. 16 is an exploded view of another detection chip according to at least one embodiment of the present disclosure; and

fig. 17 is a schematic diagram of a detection system according to at least one embodiment of the present disclosure.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without any inventive step, are within the scope of protection of the disclosure.

Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

During the design process of the microfluidic chip, the inventor hopes to integrate various functions of analysis and detection on the chip as much as possible to reduce the dependence of the chip on external operation, thereby realizing automation and integration. For example, the sample introduction part and the analysis and detection part of the microfluidic chip can be integrated together, and the automation of the detection process is realized through the structural design, so that the technical requirements on operators are reduced, the human errors are reduced, and the obtained detection data is more accurate.

At least one embodiment of the present disclosure provides a detection chip, which includes a sample application opening and at least one detection branch structure. Each of the at least one detection branch structures includes a detection section; the detection part comprises a detection groove and a reaction reagent, the detection groove is communicated with the sample adding opening, the reaction reagent is contained in the detection groove, and the detection part is configured to allow the optical detection of the reaction reagent in the detection groove.

At least one embodiment of the present disclosure provides a detection chip, which can integrate a plurality of basic structural units or components for sample detection onto the same chip, and complete the whole process of sample analysis and detection through active control or capillary action, thereby realizing an automated and integrated detection process. Therefore, the detection chip can reduce human errors possibly existing in the detection process, improve the accuracy of detection data, and meanwhile, the overall appearance of the detection chip can be thinned or miniaturized, so that the portable detection system is facilitated.

The detection chip and the detection system provided by the present disclosure are described below by several specific embodiments.

In some embodiments of the present disclosure, each of the at least one detection branch structures further includes a flow guide groove having a first end and a second end, the first end of the flow guide groove is communicated with the sample application opening, and the second end of the flow guide groove is communicated with the detection groove, so that the detection groove is communicated with the sample application opening through the flow guide groove.

Fig. 1A and 1B are a front perspective view and a back perspective view of a detection chip provided in at least one embodiment of the present disclosure, respectively, and fig. 2A and 2B are exploded views, i.e., exploded views, of the detection chip shown in fig. 1A and 1B, respectively. FIGS. 1A and 2A are top views of a detection chip showing a structure viewed from the front of the detection chip; fig. 1B and 2B are bottom views of the sense die, showing the structure as viewed from the back of the sense die.

For example, as shown in fig. 1A-2B, the detection chip 10 includes a sample application opening 110 and at least one detection branch structure 120, for example, a plurality of detection branch structures 120, and six detection branch structures 120 are shown as an example for introduction. The sample application opening 110 is used for applying a sample to be tested, such as breast milk, body fluid, blood, etc. The plurality of detecting branch structures 120 are uniformly distributed along the periphery of the sample loading opening 110, so that the plurality of detecting branch structures 120 can independently realize the detecting function.

For example, each of the plurality of detection branch structures 120 includes a diversion trench 130 and a detection part 140. Channel 130 has a first end and a second end, the first end communicating with sample addition opening 110. The sensing part 140 includes a sensing groove 141 and a reaction reagent 150, the sensing groove 141 communicates with the second end of the guide channel 130, and the reaction reagent 150 is accommodated in the sensing groove 141. The detecting part 140 is configured to allow optical detection of the reactive agent 150 in the detecting groove 141.

For example, in some embodiments, the reactive agent 150 may be in the form of a membrane as shown in fig. 1A-2B, i.e., a reactive membrane; alternatively, in some embodiments, the reactive reagent 150 may be a bulk or powdered reactive reagent, such as a lyophilized reagent that may be bulk or powdered, or the like; alternatively, in some embodiments, for example, in a case where the detection chip includes a plurality of detection branch structures, the reaction reagent contained in one part of the detection groove may be a membrane-shaped reaction film, and the reaction reagent contained in another part of the detection groove may be a block-shaped or powder-shaped reaction reagent different from the membrane-shaped reaction film, for example, which is not limited in this respect by the embodiments of the present disclosure.

The following describes a detection chip provided in an embodiment of the present disclosure, taking the reaction reagent 150 as a reaction membrane (i.e., the reaction membrane 150) as an example. It should be noted that the embodiments of the present disclosure include but are not limited thereto.

For example, in some embodiments, the detection chip further comprises an optical calibration branching structure comprising an optical path detection region configured to perform optical calibration, thereby promoting accuracy and precision of detection data obtained by optical detection.

For example, in the case that the detection chip includes a plurality of detection branch structures, the optical calibration branch structure and the plurality of detection branch structures are uniformly distributed along the periphery of the sample addition opening. The optical calibration branch structure is not provided with the reaction film, for example, other structures of the optical calibration branch structure except the reaction film can be basically the same as or similar to the detection branch structure, so that the optical calibration branch structure and the detection branch structure can be integrally arranged, and the preparation process and the preparation cost of the detection chip are simplified.

For example, taking the detection chip 10 shown in fig. 1A-2B as an example, the reaction film 150 may not be disposed in one of the detection branch structures 120 and may be used as the optical calibration branch structure of the detection chip 10 for optical calibration, and for example, the reaction film 150 (and the water absorption film 170 described below) may not be disposed in one of the detection branch structures 120 shown in fig. 2B and may be used as the optical calibration branch structure of the detection chip 10.

For example, the optical alignment branching structure includes an optical path detection area, which may be a position corresponding to the detection groove 141. Therefore, when the detection device is used to optically detect the reaction film 150 in the detection chip 10, the optical path emitted by the detection device can be calibrated through the optical calibration branching structure, for example, the irradiation angle of the optical path is calibrated, so as to improve the accuracy of the obtained detection data.

It should be noted that, in some embodiments of the present disclosure, two or more optical calibration branch structures may be provided for optical calibration according to actual requirements. For example, taking the detection chip 10 shown in fig. 1A-2B as an example, the reaction membrane 150 may not be disposed in the two detection branch structures 120 to serve as the optical calibration branch structure of the detection chip 10, and the two optical calibration branch structures may be symmetric with respect to the sample application opening 110, so as to improve the accuracy and precision of optical calibration and further improve the accuracy of the obtained detection data.

It should be noted that, in some other embodiments of the present disclosure, the detection chip may also implement calibration in other manners according to actual requirements, and accordingly, the calibration branch structure may also be other types of calibration structures besides optical calibration, for example, in other types of calibration structures, a reaction film may be disposed according to actual requirements, or no reaction film (or other structures or components that may affect calibration, such as the water absorption film 170 described below) may be disposed, which is not limited in this respect by the embodiments of the present disclosure.

For example, the reaction membrane 150 includes a detection reagent therein; the sample to be tested, which is loaded from the loading opening 110, enters the testing groove 141 through the guiding groove 130, and reacts with the testing reagent in the reaction membrane 150. In this case, the detection of the sample to be detected, for example, the presence or absence of a certain component or the amount of a certain component in the sample to be detected can be achieved by detecting, for example, a color change or the like which is reflected on the reaction film 150 after the reaction between the detection reagent and the sample to be detected by, for example, optical detection.

Therefore, the detection chip 10 provided by at least one embodiment of the present disclosure can realize integration of multiple functions such as mixing, analysis and detection, and complete the whole process of sample analysis and detection through active control and capillary action, thereby realizing an automated and integrated detection process, reducing human errors possibly existing in the detection process, and improving the accuracy of detection data. Moreover, in some embodiments, the detecting chip 10 can place the reaction films 150 comprising different detecting reagents in the detecting grooves 141 of the detecting branch structures 120, so that different detecting reagents can be used to simultaneously detect multiple indexes in the same sample, thereby shortening the detecting period of the sample and further realizing the timely detection of multiple indexes of the sample.

For example, in some examples, the sample being tested may be a liquid, such as a sample of breast milk. In the manufacturing process of the test chip 10, a desired test reagent (e.g., a coloring reagent) may be titrated in advance on the initial reaction film 150, the test reagent to be tested is dried after being immersed in the reaction film 150, and then the reaction film 150 including the test reagent is placed in the test groove of the test chip 10, thereby facilitating the storage and transportation of the test chip 10. When the detection chip 10 is used for detection, a sample is added from the sample addition opening 110, and the sample flows into the detection groove 141 through the diversion trench 130 and is mixed with the detection reagent on the reaction membrane 150 to react. Thus, the presence or absence of the analyte in the sample and the amount of the analyte are determined by detecting the reaction result (for example, by recognizing a change in color on the reaction film 150), and the detection of a certain index of the sample is realized.

For example, in at least one embodiment described above, the amount of sample injected may be 60 μ L to 80 μ L when the sample is detected using the detection chip 10. Thus, the amount of sample required for detecting a sample by the detection chip 10 can be reduced, thereby reducing or avoiding waste of the sample.

For example, in some embodiments, as shown in fig. 1A-2B, each of the detection branch structures 120 may further include a water absorption film 170, and the water absorption film 170 is accommodated in the detection groove 141 and at least partially stacked with, e.g., in direct contact with, the reaction film 150 in a thickness direction of the reaction film 150 (i.e., in a direction perpendicular to the first substrate 101 described below).

For example, the liquid storage amount of each water-absorbent film 170 may be 10. mu.L to 50. mu.L, for example, 30. mu.L. The water absorption film 170 can contain a certain amount of sample, and after the amount of liquid stored in the water absorption film 170 exceeds the amount of liquid stored in the water absorption film, the water absorption film 170 can not absorb the sample any more, so that the sample quantification function is achieved, and the sample amount control is facilitated.

For example, the material of the water absorbing film 170 may include glass fiber, cotton fiber, or a composite fiber of glass fiber and cotton fiber.

It should be noted that, in some other embodiments of the present disclosure, the detection chip may not employ a water absorption film, and in this case, the reaction film 150 may simultaneously function as a sample quantification medium and a reaction carrier.

It should be noted that, in the embodiment of the present disclosure, the lengths, widths, depths, and the like of the diversion trenches 130 are the same as each other, so that the amounts of the samples flowing into the different detection grooves 141 can be controlled to be relatively uniform, which is beneficial to controlling the amount of the samples in the detection grooves 141, and meanwhile, the reaction time between the samples in the different detection grooves 141 and the detection reagent on the reaction film 150 can be relatively uniform, thereby improving the accuracy of the obtained detection data.

In other embodiments of the present disclosure, for example, in the case of simultaneously detecting multiple indexes in the same sample by using different detection reagents, the diversion trenches 130 may have different sizes, for example, different lengths, widths, or depths, according to different indexes of the sample to be detected, so as to control the amount of the sample in different detection grooves 141 and the reaction time between the sample in different detection grooves 141 and the detection reagent on the reaction film 150, thereby obtaining multiple more accurate detection data according to actual requirements.

It should be noted that, in the embodiment of the present disclosure, the specific length, width, depth, etc. of the diversion trench 130 may be determined according to actual requirements, such as sample amount, sample characteristics, etc. For example, the length of the diversion trench 130 can be reduced appropriately to reduce or avoid waste of the sample during the flowing process, while achieving the effect of flowing the sample from the sample application opening 110 into the detection groove 141.

In some other embodiments of the present disclosure, the diversion trench 130 may not be disposed in the detection chip 10, for example, the detection groove 141 is directly connected to the sample application opening 110 for communication, so that waste of the sample during the flowing process can be further reduced or avoided. At this time, in some examples, the sample application opening 110 may have a flow guide structure therein, which is respectively communicated with the plurality of detection grooves 141, so that the sample applied to the sample application opening 110 can uniformly flow into the plurality of detection grooves 141.

For example, in some embodiments, the size of the plurality of detection grooves 141 can be selected according to the reagent condition, such as different indexes of the sample to be detected, the amount of the sample to be detected, and the type of the detection reagent. According to practical requirements, the sizes and shapes of the plurality of detection grooves 141 may be the same or different, and embodiments of the present disclosure are not limited thereto.

For example, in some embodiments, the detection chip 10 further includes a first substrate 101 and a second substrate 102. The first substrate 101 has a first surface and a second surface opposite to each other, the sample loading opening 110 is a through hole in the first substrate 101, and the guiding gutter 130 and the detecting groove 141 are formed on the first surface of the first substrate 101. The second substrate 102 is laminated on the first surface of the first substrate 101 and allows optical inspection at a position corresponding to the inspection groove 141.

For example, fig. 3A and 3B are front and back perspective views of the first substrate of the detection chip shown in fig. 1A and 1B, respectively. Fig. 3A is a top view of the first substrate 101, showing a front structure of the first substrate, and fig. 3B is a bottom view of the first substrate 101, showing a back structure of the first substrate. Fig. 4 is a schematic plan view of a first surface of a first substrate of the detection chip shown in fig. 1A and 1B, and fig. 5 is a schematic plan view of a second substrate of the detection chip shown in fig. 1A and 1B.

For example, as shown in fig. 1A-5, when the detection chip has a water absorption film 170, the water absorption film 170 may be disposed on a side of the reaction film 150 away from the second substrate 102, i.e., between the reaction film 150 and the detection groove 141.

For example, the second substrate 102 has a detection through hole 160 at a position corresponding to the detection groove 141, and the reaction film 150 in the detection groove 141 can be optically detected through the detection through hole 160 to obtain a detection index of the sample.

For example, the detection through hole 160 penetrates the second substrate 102, and the diameter of the detection through hole 160 may be set to 2mm to 10mm, further 0.5mm to 4mm, and for example, may be set to 3 mm. Therefore, optical detection can be realized through the detection through holes 160, and mutual interference between light rays emitted to different detection through holes 160 can be weakened or avoided, so that the accuracy and precision of optical detection are further improved.

For example, the portions of the second substrate 102 other than the detection through holes 160 may be configured as opaque portions, so that interference between light rays emitted to different detection through holes 160 and crosstalk between the detection through holes 160 can be reduced or avoided. For example, the opaque portion of the second substrate 102 may be formed by dyeing or the like.

For example, a light shielding layer may be disposed on a side of the second substrate 102 away from the first substrate 101, and the light shielding layer covers the surface of the second substrate 102 away from the first substrate 101 except for the position having the detection through hole 160, that is, the light shielding layer covers the surface of the second substrate 102 away from the first substrate 101 and exposes the detection through hole 160, so that interference between light rays emitted to different detection through holes 160 can be reduced or avoided, and crosstalk between the detection through holes 160 can be reduced or avoided. For example, the light shielding layer is made of an opaque material, and the light shielding layer may be disposed on the surface of the second substrate 102 by, for example, pasting, printing, and the like, and the embodiment of the present disclosure does not limit the specific manner of forming the light shielding layer.

For example, the light shielding layer may be disposed on a side of the second substrate 102 close to the first substrate 101, that is, the light shielding layer covers a surface of the second substrate 102 close to the first substrate 101 and exposes the detection through holes 160, so that interference between light rays emitted to different detection through holes 160 can be reduced or avoided. Alternatively, in some embodiments of the disclosure, a side of the second substrate 102 away from the first substrate 101 and a side of the second substrate 102 close to the first substrate 101 may both be provided with a light shielding layer, and the two light shielding layers respectively cover other portions of the surface of the second substrate 102 away from and close to the first substrate 101 except for the position having the detection through hole 160, so that the light rays emitted to different detection through holes 160 may be better reduced or prevented from interfering with each other, and further, the optical crosstalk between the detection through holes 160 may be reduced or prevented.

For example, in some other embodiments of the present disclosure, the second substrate of the detection chip may not be provided with a detection through hole, and in this case, the second substrate may be made of a transparent material that allows light to pass through, so that the reaction film in the detection groove can be optically detected directly through the second substrate.

For example, the second substrate may be made of a transparent material that allows light to pass through, and the detection window may be formed by printing or pasting an opaque light shielding layer on a surface of the second substrate away from the first substrate to allow light to pass through only the detection window. For example, in some embodiments, the light shielding layer may also be disposed on a surface of a side of the second substrate close to the first substrate, or both the surface of the side of the second substrate close to the first substrate and the surface of the side far from the first substrate may be disposed with the light shielding layer, which is not limited in this respect by the embodiments of the present disclosure. The embodiment of the present disclosure does not limit the specific material used for the second substrate, as long as the optical detection of the reaction film in the detection groove of the first substrate can be performed through the second substrate or the detection through hole formed in the second substrate.

It should be noted that, in the embodiment of the present disclosure, the number of the detection grooves 141 provided on the first substrate 101 and the number of the detection through holes 160 provided on the second substrate 102 and corresponding to the detection grooves 141 are only exemplary, and the specific number of the detection grooves 141 and the detection through holes 160 is not limited in the embodiment of the present disclosure. For example, 1-20 sensing grooves 141 may be provided on the first substrate 101, and correspondingly, 1-20 sensing through holes 160 may be provided on the second substrate 102.

For example, the material of the second substrate 102 may include one or more of polymethylmethacrylate, polystyrene, polycarbonate, and polyethylene terephthalate. For example, the material of the first substrate 101 may include one or more of polymethyl methacrylate, polystyrene, and polycarbonate, or may be other materials with high light transmittance, and the like. The embodiment of the present disclosure does not specifically limit the material of the first substrate 101 and the second substrate 102.

For example, the first substrate 101 and the second substrate 102 may be bonded (i.e., two materials are bonded together by van der waals force, molecular force, atomic force, or the like), welded, bonded, or snapped together, thereby simplifying the processes of the detection chip 10, such as processing and assembly, and reducing the manufacturing cost of the detection chip 10. For example, the first substrate 101 and the second substrate 102 may be bonded by ultrasonic bonding, thermal compression bonding, laser welding, ultrasonic welding, or silicone sealing. The embodiment of the present disclosure does not limit a specific bonding manner between the first substrate 101 and the second substrate 102.

For example, the first substrate 101 may be circular, rectangular or other suitable shape, and the second substrate 102 may also be circular, rectangular or other suitable shape.

For example, in the case where the shape of the first substrate 101 is a circle, the diameter of the first substrate 101 may be 3cm to 15cm, and further may be 3cm to 5 cm. Since the detecting chip 10 is required to be matched with a detecting device, the first substrate 101 with the above size can reasonably reduce the space occupied by the detecting chip 10, so that the detecting chip 10 can be designed to be thin, thereby facilitating the matching use of the detecting chip 10 and the detecting device, and reducing the cost of transportation, packaging, storage and the like of the detecting chip 10. For example, in the case where the second substrate 102 has a circular shape, the diameter of the second substrate 102 may be 3cm to 15cm, and further may be 3cm to 5 cm. Since the detecting chip 10 is required to be matched with a detecting device, the second substrate 102 with the above size can reasonably reduce the space occupied by the detecting chip 10, so that the detecting chip 10 can be designed to be thin, thereby facilitating the matching use of the detecting chip 10 and the detecting device, and reducing the cost of transportation, packaging, storage and the like of the detecting chip 10.

For example, in the case where the first substrate 101 has a rectangular shape, the diagonal line of the first substrate 101 may be 3cm to 15cm, and further may be 3cm to 5 cm. For example, the rectangular first substrate 101 can simplify the manufacturing process of the detection chip 10, and reduce the processing process and the precision requirement of the detection chip 10, thereby reducing the manufacturing cost of the detection chip 10. In the case where the second substrate 102 has a rectangular shape, the diagonal line of the second substrate 102 may be 3cm to 15cm, and further may be 3cm to 5 cm. For example, the rectangular second substrate 102 can simplify the manufacturing process of the test chip 10, and reduce the processing process and the precision requirement of the test chip 10, thereby reducing the manufacturing cost of the test chip 10.

For example, the first and

second substrates

101 and 102 may have the same or similar shape and size to each other to facilitate bonding of the first and

second substrates

101 and 102.

For example, the thickness of the first substrate 101 may be 0.5mm to 10mm, such as 3mm or 5mm, etc. For example, the thickness of the second substrate 102 may be 0.1mm to 5mm, such as 0.5mm or 2mm, etc. Therefore, the space occupied by the detection chip 10 can be reasonably reduced, the detection chip 10 is designed to be thin, the cost of transportation, packaging, storage and the like of the detection chip 10 is reduced, and the matching use of the detection chip 10 and a detection device is facilitated. In addition, the above numerical range is also useful for detecting the processing and preparation of the chip 10, and is convenient for a user to grasp with one hand, fix or pick with an apparatus, and the like.

For example, in one example, the first substrate 101 and the second substrate 102 have a diameter of 3.5cm, the first substrate 101 has a thickness of 1.5mm, and the second substrate 102 has a thickness of 1 mm. Therefore, the detection chip 10 is designed to be thin, and a user can conveniently hold the detection chip by one hand and fix or pick the detection chip by equipment, the preparation process of the detection chip 10 can be simplified, the processing process and the precision requirement of the detection chip 10 are reduced, and the processing and the preparation of the detection chip 10 are facilitated.

For example, as shown in fig. 1A-5, in some embodiments, the detection chip 10 can further include a sample addition projection 190. The sample addition protrusion 190 protrudes from the first surface of the first substrate 101 in a direction away from the second substrate 102, one end of the sample addition protrusion 190 is connected to the sample addition opening 110, and the other end can be communicated with the atmosphere. Application of sample bulge 190 can play effects such as sample accommodation and water conservancy diversion to make the sample flow to application of sample opening 110 through application of sample bulge 190 fast, thereby be convenient for inject the sample into in application of sample opening 110, increase the sample volume that detects chip 10, and be convenient for detect chip 10 for example take and place etc..

For example, the sample addition projection 190 comprises a conical cavity and communicates the sample addition opening 110 with the atmosphere. For example, the conical angle of the conical cavity (e.g., the angle between the centerline and the sidewall of the cone) may be 30-75 °, with a volume of 50-200 μ L. For example, the conical cavity of the sample addition bulge 190 comprises a first end and a second end opposite to each other, the first end is connected with the sample addition opening 110 and has a diameter of 0.5mm-5mm, and the second end has a diameter of 1mm-20 mm. The height of the conical cavity of the sample-adding projection part 190 protruding the first substrate 101 is 1mm-20 mm. Thus, when the detection chip 10 is used in combination with, for example, a detection device, the sample addition projection 190 can occupy a reasonable space to match the size of the detection device, thereby facilitating the use of the detection chip 10 in combination with the detection device. In addition, the sample-adding protrusion 190 determined by the above numerical range can better perform the functions of sample accommodation, flow guidance and the like, for example, the sample can uniformly flow to the sample-adding opening 110 at a reasonable speed, thereby facilitating the injection of the sample into the sample-adding opening 110.

For example, in one example, the diameter of the end of the sample addition protrusion 190 that meets the sample addition opening 110 is 3mm, the diameter of the other end is 10mm, and the height of the sample addition protrusion 190 that protrudes from the first substrate 101 is 1.5 mm. Thus, the sample can rapidly flow to the sample-adding opening 110 through the sample-adding projection 190, and the detection chip 10 can be taken, placed and used in cooperation with a detection device. Meanwhile, the preparation process of the sample adding bulge 190 can be simplified, so that the processing process and the precision requirement are reduced.

For example, in some embodiments, as shown in fig. 4, the sample loading opening 110 includes a first main body 111 and a first protrusion 112 protruding from the first main body 111 to the flow guide groove 130, and the first protrusion 112 is in communication with the flow guide groove 130 to facilitate rapid flow of the sample into the flow guide groove 130 through the first protrusion 112.

For example, the diameter of the first body 111 may be set to 1mm-10mm, such as 5mm or 7mm, etc. Therefore, the sample can be ensured to uniformly and rapidly flow into the diversion trench 130, and the space required by the detection groove 141 can be ensured.

For example, the shape of the first body 111 may be a circle, a square, or another suitable shape, and the first protrusion 112 may be a square, a pointed shape, or another shape, which is not limited in this disclosure. For example, when the shape of the first body 111 is a square, the diagonal length of the first body 111 may be set to 1mm to 10mm, for example, 5mm or 7mm, etc. For example, the first body 111 having a circular or square shape can simplify the manufacturing process of the test chip 10, and reduce the processing process and the precision requirement of the test chip 10, thereby reducing the manufacturing cost of the test chip 10.

For example, the inner wall of the guiding gutter 130 may be configured to have hydrophilicity, for example, a contact angle of liquid on the inner wall of the guiding gutter 130 is less than 90 °, so as to facilitate the sample to rapidly enter the guiding gutter 130, and the sample can rapidly flow into the detection groove 141 through the guiding gutter 130 to be mixed with the reaction membrane 150, thereby shortening the detection time of the sample and improving the accuracy of the obtained detection data. For example, in the process of manufacturing the test chip, a hydrophilic agent may be injected into the guiding groove 130, and the guiding groove 130 is soaked by the hydrophilic agent for 1 minute and then the hydrophilic agent is discharged by air, so that the inner wall of the guiding groove 130 may be set to have hydrophilicity.

It should be noted that the contact angle of the liquid on the inner wall of the guiding groove 130 is the included angle formed by the tangent line along the liquid drop surface and the inner wall surface of the guiding groove 130. For example, when the contact angle of the liquid on the inner wall of channel 130 is less than 90 °, the inner wall of channel 130 may be considered to have hydrophilicity. The smaller the value of the contact angle of the liquid on the inner wall of channel 130, the better the wettability of the inner wall of channel 130, e.g. when the contact angle of the liquid on the inner wall of channel 130 is 0 °, the inner wall material of channel 130 is completely wetted. Embodiments of the present disclosure do not limit a specific value of a contact angle of the liquid on the inner wall of the guide channel 130 as long as the contact angle is less than 90 ° to make the inner wall of the guide channel 130 hydrophilic.

For example, in some embodiments, the height of channels 130 may be set to 0.1mm-1.5mm, such as 0.5 mm; the width of the guide groove 130 may be set to 0.1mm to 2mm, for example, 0.5 mm. Thereby, it is helpful to control the amount of sample flowing into the detection groove 141 through the guide groove 130, and waste of sample during the flowing process is reduced or avoided.

For example, in some embodiments, the ratio of the height of channels 130 to the width of channels 130 may be set to 1:1 to 10:1, e.g., 2: 1. Thus, in some embodiments, when the first substrate 101 and the second substrate 102 are bonded by a bonding method, for example, the first surface of the first substrate 101 and the second substrate 102 are bonded by an adhesive, in this case, the adhesive may be made of a hydrophobic material, and since the hydrophobic adhesive is provided between the first surface of the first substrate 101 and the bonding surface of the second substrate 102, by increasing the ratio of the height of the flow guide groove 130 to the width of the flow guide groove 130, the contact area of the sample with the hydrophobic adhesive on the bonding surface of the second substrate 102 when the sample flows through the flow guide groove 130 may be reduced, thereby achieving a rapid flow of the sample in the flow guide groove 130.

For example, in one example, channel 130 has a height of 1mm and channel 130 has a width of 0.5 mm. Therefore, the rapid flow of the sample in the diversion trench 130 is facilitated, the waste of the sample in the flow process can be reduced or avoided, the preparation process of the diversion trench 130 can be simplified, and the requirements on the processing process and the precision are reduced.

It is noted that in some embodiments, the second end of the flow guide groove 130 (i.e., the end communicating with the detection groove 141) is in contact with the reaction membrane 150, so as to facilitate the sample to be immersed into the reaction membrane 150, and to allow the sample to sufficiently react with the detection reagent in the reaction membrane 150, for example, a portion of the reaction membrane 150 may be located in the flow guide groove 130. In some embodiments, the reaction film 150 may not contact the second end of the flow guide groove 130, which is not limited in the embodiments of the present disclosure.

For example, in the embodiment of the disclosure, the structures of the detection chip 10, such as the sample addition protrusion 190, the sample addition opening 110, the diversion trench 130, the detection groove 141, and the like, can be integrally formed by, for example, an injection molding process, so as to simplify the manufacturing process of the detection chip 10.

For example, in some embodiments, the reaction membrane 150 includes a matrix material, which may include glass fibers, cotton fibers, or a composite of glass fibers and cotton fibers, and a detection reagent distributed in the matrix material.

For example, in the detection chip of some embodiments, the reaction film 150 is in a compressed state in a thickness direction by the two substrates in a state where the first substrate 101 and the second substrate 102 are bonded. For example, the thickness of the reaction film 150 in the relaxed state may be equal to or slightly greater than the height of the detection groove 141, for example, the ratio of the thickness of the reaction film 150 in the relaxed state to the height of the detection groove 141 may be 1:1-1:0.5, and after the first substrate 101 and the second substrate 102 are combined, since the reaction film 150 is compressed along the thickness direction, the surface of the side of the reaction film 150 facing the second substrate 102 and the first surface of the first substrate 101 may be located on the same horizontal plane, so that the reaction film 150 and the second substrate 102 are in close contact, and a liquid storage phenomenon caused by a gap between the reaction film 150 and the first substrate 101 or the second substrate 102 may be avoided. Therefore, the entered sample can be more fully immersed into the reaction membrane 150 and more fully reacted with the detection reagent in the reaction membrane 150, and the accuracy of the obtained detection data is improved.

For example, in some embodiments of the present disclosure, the compression amount of the reaction film 150 may be set to 10% to 40%, so as to facilitate the reaction film 150 to be in close contact with the second substrate 102, thereby reducing or avoiding a liquid storage phenomenon caused by a gap between the reaction film 150 and the first substrate 101 or the second substrate 102.

For example, the reaction membrane 150 generally has the same or similar shape as the detection groove 141, so that the reaction membrane 150 is accommodated in the detection groove 141 and the uniform mixing between the reaction membrane 150 and the sample is facilitated.

For example, FIG. 6 shows an exemplary structure of a reaction membrane of the detection chip shown in FIGS. 1A and 1B.

For example, as shown in fig. 4 and 6, in some embodiments, the reactive membrane 150 includes a membrane body 151 and a second protrusion 152. The film body 151 and the detection groove 141 have the same shape and are circular. The second protrusion 152 protrudes from the film main body 151 to the guide groove 130, and at least a portion of the second protrusion 152 is located in the guide groove 130, so that the sample can be introduced into the detection groove 141 from the guide groove 130, and the sample can be sufficiently immersed in the reaction film 150, so that the sample can sufficiently react with the detection reagent in the reaction film 150, and the accuracy of the obtained detection data can be improved.

For example, the diameter of the detection groove 141 may be set to 3mm to 15mm, and the diameter of the film body 151 of the reaction film 150 is equal to or smaller than the diameter of the detection groove 141. Thereby, the reaction membrane 150 can be better accommodated in the detection groove 141, thereby facilitating uniform mixing between the reaction membrane 150 and the sample.

For example, in some other embodiments of the present disclosure, the whole of the reaction membrane may also be configured to be the same as the shape of the detection groove, i.e., the reaction membrane may only have the membrane main body portion and not have the protruding portion (e.g., the second protruding portion 152), which is not limited by the embodiments of the present disclosure.

For example, in some other embodiments of the present disclosure, the reaction membrane may be further configured as a polygon, and one corner of the polygon is directly connected to the second end of the flow guide groove. Accordingly, the detection groove is a polygon having the same or similar shape as the reaction membrane.

For example, the reaction film may be disposed in a regular shape such as a triangle, a square, a diamond, or the like, or may be disposed in an irregular shape, or the like, which is not limited in this regard by the embodiments of the present disclosure.

For example, fig. 7A and 7B are a front perspective view and a back perspective view, respectively, of another detection chip provided in at least one embodiment of the present disclosure. For example, fig. 7A is a top view of the detection chip, and fig. 7B is a bottom view of the detection chip. Fig. 8 is an exploded view, i.e., an exploded view, of the sense die shown in fig. 7A and 7B.

For example, as shown in fig. 7A-8, the detecting chip 20 includes a sample loading opening 210 and a plurality of detecting branch structures 220, and the plurality of detecting branch structures 220 are uniformly distributed along the periphery of the sample loading opening 210. Each of the plurality of detection branch structures 220 includes a flow guide groove 230 and a detection part 240. The flow guide groove 230 has a first end and a second end, and the first end is communicated with the sample adding opening 210. The sensing part 240 includes a sensing groove 241 and a reaction reagent (e.g., a reaction film 250), the sensing groove 241 communicates with the second end of the guide channel 230, the reaction film 250 is received in the sensing groove 241, and the sensing part 240 is configured to allow optical sensing of the reaction film 250 positioned in the sensing groove 241.

For example, as shown in FIGS. 7A to 8, the reaction membrane 250 of the detection chip 20 is provided in a polygonal shape, such as a square or a diamond shape, and accordingly, the shape of the detection groove 241 is also provided in a square or a diamond shape.

For example, the side length of the detection groove 241 may be set to 3mm to 15mm, such as 5mm or 10mm, and the side length of the reaction film 250 is equal to or less than the side length of the detection groove 241. Thereby, the reaction membrane 250 can be better accommodated in the detection groove 241, thereby facilitating uniform mixing between the reaction membrane 250 and the sample.

For example, in the case where the shape of the detection groove 341 is set to be square, the diagonal line of the square may be set to be 1mm to 20mm, for example, 10mm or 15mm, or the like. For example, in the case where the shape of the detection groove 341 is set to a diamond shape, two diagonal lines of the diamond shape may be set to 1mm to 20mm, respectively; for example, one diagonal is 10mm long, while the other diagonal is 6mm long. For example, the length ratio between two diagonal lines of the diamond shape can be set to 1:1-1: 2. For example, the included angle between two adjacent sides of the diamond shape may be set to be less than or equal to 60 °, so as to facilitate the sample to be sufficiently immersed into the reaction membrane 250 along the periphery of the reaction membrane 250, so that the sample can sufficiently react with the detection reagent in the reaction membrane 250, thereby improving the accuracy of the obtained detection data.

For example, one corner of the reaction membrane 250 directly communicates with the second end of the flow guide groove 230 to introduce the sample from the flow guide groove 230 into the detection groove 241, and the sample can be sufficiently immersed into the reaction membrane 250 to sufficiently react with the detection reagent in the reaction membrane 250, thereby improving the accuracy of the obtained detection data.

Fig. 9A and 9B are front and rear perspective views of the first substrate of the detection chip shown in fig. 7A and 7B, respectively. For example, fig. 9A is a top view of the first substrate 201, and fig. 9B is a bottom view of the first substrate 201. Fig. 10 is a schematic plan view of the first surface of the first substrate of the detection chip shown in fig. 7A and 7B.

For example, as shown in fig. 7A to 10, the detection chip 20 further includes a first substrate 201 and a second substrate 202. The first substrate 201 has a first surface and a second surface opposite to each other, the sample loading opening 210 is a through hole in the first substrate 201, and the diversion trench 230 and the detection groove 241 are formed on the first surface of the first substrate 201. The second substrate 202 is laminated on the first surface of the first substrate 201 and allows optical detection at a position corresponding to the detection groove 241.

For example, the second substrate 202 has a detection through hole 260 at a position corresponding to the detection groove 241, and the reaction film 250 in the detection groove 241 can be optically detected through the detection through hole 260 to obtain different detection indexes of the sample.

For example, in the embodiment of the present disclosure, the shape of the detection through hole 260 is a circle, while in some other embodiments of the present disclosure, the shape of the detection through hole 260 may also be set to be, for example, a square, a diamond, or other shape, which is the same as the shape of the detection groove 241, and the embodiment of the present disclosure is not limited thereto.

For example, as shown in fig. 10, the sample application opening 210 includes a first main body 211 and a first protrusion 212 protruding from the first main body 211 to the flow guide groove 230, and the first protrusion 212 is in communication with the flow guide groove 230, so that the sample flows into the flow guide groove 230 through the first protrusion 212.

It should be noted that, for the materials, specific structural parameters, and the like of the first substrate 201 and the second substrate 202, reference may be made to the corresponding descriptions of the first substrate 101 and the second substrate 102 of the detection chip 10 in the foregoing embodiments, and no further description is given here. For specific structural parameters, materials, functions, and the like of the detection chip 20, reference may be made to the corresponding descriptions of the detection chip 10 in the above embodiments, which are not described herein again.

For example, in some embodiments of the present disclosure, the detection portion of the detection chip may further include a flow guide groove located at a periphery of the detection groove, e.g., the flow guide groove may at least partially surround the detection groove. The flow guide groove is formed in the first surface of the first substrate and communicated with the detection groove, and the height of at least part of the flow guide groove is smaller than that of the detection groove. Therefore, the sample can be rapidly infiltrated along part of the peripheral area of the reaction film through the flow guide groove, capillary force is generated between the flow guide groove and the reaction film to play a role in drainage, and the sample is infiltrated from the periphery of the reaction film to the central area of the reaction film, so that the mixing speed between the sample and the reaction film is accelerated, and the detection time is further shortened. In addition, in the case that the sample is infiltrated from the periphery of the reaction membrane, due to the capillary action, the amount of the sample infiltrated into the central area of the reaction membrane is increased, so that the detection result (such as color development) of the central area of the reaction membrane is more obvious, the subsequent observation of the detection result is facilitated, and the accuracy of the obtained detection data is improved.

For example, in some embodiments of the present disclosure, the diversion groove surrounds the periphery of the detection groove, i.e., surrounds all the peripheral regions of the detection groove, so that the sample can more rapidly infiltrate through the diversion groove along the periphery of the reaction membrane and then infiltrate from the periphery of the reaction membrane to the center of the reaction membrane, thereby further increasing the mixing speed between the sample and the reaction membrane and more significantly shortening the required detection time. In addition, under the condition that the sample is simultaneously soaked from the periphery of the reaction film, due to the capillary action, the amount of the sample soaked to the center of the reaction film is increased, so that the detection result (such as color development) of the center of the reaction film is most obvious, the observation of the subsequent detection result is facilitated, and the accuracy of the obtained detection data is further improved.

It should be noted that, in some embodiments of the present disclosure, the flow guiding groove may also intersect or partially overlap with the detection groove in a direction perpendicular to the first surface of the first substrate, and the embodiments of the present disclosure are not limited thereto.

For example, fig. 11 is an exploded view, i.e., an exploded view, of another detection chip provided in at least one embodiment of the present disclosure. Fig. 12 is a schematic structural diagram of the first substrate of the detection chip shown in fig. 11. For example, fig. 12 is a bottom view of the first substrate 301 of the detection chip 30 shown in fig. 11. It should be noted that, except for the liquid storage through hole 380 and the flow guide groove 342, the structure and function of the detection chip 30 shown in fig. 11 are substantially the same as or similar to those of the detection chip 20 shown in fig. 7A-8, and are not repeated herein.

For example, as shown in conjunction with fig. 11 and 12, the detecting part 340 of the detecting chip 30 includes a reaction reagent (e.g., a reaction film 350), a detecting groove 341, and flow

guide grooves

342 and 343 surrounding the detecting groove 341.

Fig. 13A is a schematic partial structure diagram of the first substrate of the detection chip shown in fig. 12, for example, a schematic partial structure diagram of the first substrate 301 of the detection chip 30 including the detection portion 340, fig. 13B is a schematic sectional structure diagram along a-a 'line in fig. 13A, and fig. 13C is a schematic sectional structure diagram along a B-B' line in fig. 13A. Note that, for the sake of brevity and clarity, the reaction film 350 of the detection section 340 is not shown in fig. 12 to 13C.

For example, as shown in fig. 11 to 13C, the

flow guiding grooves

342 and 343 are formed on the first surface 3011 of the first substrate 301 and communicate with the detection groove 341, for example, one end of the flow guiding groove 342 is connected to the flow guiding groove 330, and the other end is connected to the flow guiding groove 343. Therefore, when the sample flows into the detection part 340 through the flow guide groove 330, the sample flows into the

flow guide grooves

342 and 343, rapidly infiltrates around the reaction membrane 350 through the

flow guide grooves

342 and 343, and infiltrates from the periphery of the reaction membrane 350 to the center of the reaction membrane 350, thereby increasing the mixing speed between the sample and the reaction membrane 350 and shortening the required detection time. Moreover, due to the capillary action, the amount of the sample infiltrated into the center of the reaction membrane 350 is increased, so that the detection result (such as color development) of the center of the reaction membrane 350 is more obvious, the observation of the subsequent detection result is facilitated, and the accuracy of the obtained detection data is improved.

For example, as shown in fig. 13A and 13B, the longitudinal sections of the diversion groove 342 and the detection groove 341 are entirely stepped, so that a larger gap for the sample to flow can be formed between the diversion groove 342 and the reaction film 350, thereby further increasing the flow speed of the sample in the diversion groove 342, so that the sample can rapidly infiltrate along the periphery of the reaction film 350, and then infiltrate from the periphery of the reaction film 350 to the center of the reaction film 350, further improving the infiltration effect of the center of the reaction film 350, and making the detection result (e.g., color development) of the center of the reaction film 350 more obvious.

For example, as shown in fig. 13A and 13B in combination, the height H2 of the flow guide groove 342 is smaller than the height H1 of the detection groove 341. For example, the height H1 of the detection groove 341 may be set to 0.2mm to 5mm, such as 2mm or 3mm, so that in a state where the first substrate 301 and the second substrate 302 are bonded, the reaction film 350 is in a compressed state compressed by the two substrates in the thickness direction, and a surface of one side of the reaction film 350 facing the second substrate 302 and the first surface 3011 of the first substrate 301 may be located on the same horizontal plane, so that the reaction film 350 is in close contact with the second substrate 302, and a liquid storage phenomenon caused by a gap generated between the reaction film 350 and the first substrate 301 or the second substrate 302 may be avoided. The difference between the height H2 of the flow guide groove 342 and the height H1 of the detection groove 341 is 0.1mm to 1mm, such as 0.5mm or 0.8mm, so that the sample can rapidly flow in the detection groove 341, thereby more rapidly infiltrating along the periphery of the reaction membrane 350.

For example, the width D1 of the diversion groove 342 may be set to 0.1mm-1mm, such as 0.5mm or 0.7mm, etc. Therefore, a sufficient and appropriate gap for the sample to flow is formed between the flow guide groove 342 and the reaction film 350, so as to facilitate the sample to infiltrate from the periphery of the reaction film 350 to the center of the reaction film 350, improve the infiltration effect of the center of the reaction film 350, and make the detection result (e.g., color development) of the center of the reaction film 350 more obvious.

For example, in the case where the shape of the detection groove 341 is set to be square, the diagonal line of the square may be set to be 1mm to 20mm, for example, 10mm or 15mm, or the like. For example, in the case where the shape of the detection groove 341 is set to a diamond shape, two diagonal lines of the diamond shape may be set to 1mm to 20mm, respectively; for example, one diagonal is 10mm long, while the other diagonal is 6mm long. For example, the length ratio between two diagonal lines of the diamond shape can be set to 1:1-1: 2. For example, an included angle between two adjacent sides of the diamond shape may be set to be less than or equal to 60 °, so as to facilitate the sample to infiltrate toward the center of the reaction membrane 350 along the periphery of the reaction membrane 250, so that the sample may be sufficiently immersed in the reaction membrane 250, thereby improving the accuracy of the obtained detection data.

For example, in some other embodiments of the present disclosure, the diversion groove 342 may also be designed to be stepped, including a multi-step structure, which is not limited by the embodiments of the present disclosure.

For example, as shown in conjunction with fig. 13A and 13C, the flow guide grooves 343 are provided in a ramp-like structure. For example, the flow guiding groove 343 includes a flow guiding wall 344 having a slope shape, the flow guiding wall 344 is inclined with respect to the first surface 3011 of the first substrate 301, a first end 3441 of the flow guiding wall 344 is connected to the first surface 3011 of the first substrate 301, and a second end 3442 of the flow guiding wall 344 is connected to a side surface of the detection groove 341. Since a gap for the sample to flow is formed between the flow guide groove 343 and the reaction membrane 350 is small compared to the flow guide groove 342, the flow velocity of the sample in the flow guide groove 343 is slightly smaller than the flow velocity of the sample in the flow guide groove 342. Therefore, after the sample flows into the diversion groove 343 through the diversion groove 342, the sample can be weakened or prevented from being excessively remained in the diversion groove 343, so that the infiltration speed of the sample from the periphery of the reaction membrane 350 to the center of the reaction membrane 350 is further increased, the amount of the sample infiltrated into the center of the reaction membrane 350 is increased, the detection result (such as color development) of the center of the reaction membrane 350 is more obvious, and the observation of the subsequent detection result is more facilitated.

It should be noted that, in some other embodiments of the present disclosure, the flow guide groove 343 may be configured in other suitable structures as long as the space formed between the flow guide groove 343 and the reaction membrane 350 for the sample to flow is smaller than or equal to the space formed between the flow guide groove 342 and the reaction membrane 350 for the sample to flow, and the embodiments of the present disclosure are not limited thereto.

For example, as shown in fig. 12, fig. 13A and fig. 13C, the second ends 3442 of the flow guide walls 344 are not parallel to the first surface 3011 of the first substrate 301, e.g., the second ends 3442 of the flow guide walls 344 are inclined with respect to the first surface 3011 of the first substrate 301 and extend toward the first surface 3011 of the first substrate 301 along the flow direction of the sample (e.g., along a direction away from the sample addition opening 310). That is, the surface area of the flow guide wall 344 (i.e., the contact area between the flow guide wall 344 and the sample) is gradually reduced in the flow direction of the sample, for example, in a direction away from the sample application opening 310, so that the space formed between the flow guide groove 343 and the reaction membrane 350 for the sample to flow is gradually reduced in the flow direction of the sample. Therefore, the speed of the sample infiltrating from the periphery of the reaction film 350 to the center of the reaction film 350 is further increased, the amount of the sample infiltrating to the center of the reaction film 350 is further increased to a certain extent, and further, the detection result (such as color development) of the center of the reaction film 350 is more obvious, which is more beneficial to the observation of the subsequent detection result, so that the accuracy of the obtained detection data is improved.

It should be noted that, in some other embodiments of the present disclosure, the flow guide wall 344 may also be provided with other suitable profile shapes as long as the contact area between the flow guide wall 344 and the sample is gradually reduced along the flow direction of the sample, and the embodiments of the present disclosure are not limited to this.

For example, the maximum height H4 of the diversion groove 343 is smaller than the height H1 of the detection groove 341, i.e., the distance between the second end of the diversion wall 344, which meets the side surface of the detection groove 341, and the first surface 3011 of the first substrate 301 is smaller than the height H1 of the detection groove 341. Therefore, a gap for the sample to flow is formed between the flow guide groove 343 and the reaction membrane 350, so that the sample can rapidly infiltrate along the periphery of the reaction membrane 350 and then infiltrate from the periphery of the reaction membrane 350 to the center of the reaction membrane 350, thereby improving the infiltration effect of the center of the reaction membrane 350 and making the detection result (e.g., color development) of the center of the reaction membrane 350 more obvious.

For example, a difference between the distance between the second end of the guide wall 344 and the first surface 3011 of the first substrate 301 and the height of the detection groove 341 (i.e., a difference between H4 and H1) may be set to 0.1mm to 1 mm. Thus, a proper gap for the sample to flow can be formed between the flow guide groove 343 and the reaction membrane 350, thereby improving the wetting effect of the center of the reaction membrane 350.

For example, the width D2 of the flow guide groove 343 may be set to 0.1mm to 1mm, such as 0.5mm or 0.7mm, so that a proper gap for the sample to flow is formed between the flow guide groove 343 and the reaction membrane 350, thereby improving the wetting effect of the center of the reaction membrane 350.

It should be noted that, in the above-mentioned embodiment shown in fig. 11-13C, the width D1 of the flow guiding groove 342 is the same as the width D2 of the flow guiding groove 343, and the height H2 of the flow guiding groove 342 is the same as the maximum height H4 of the flow guiding groove 343; in other embodiments of the present disclosure, the width D1 of the flow guiding groove 342 and the width D2 of the flow guiding groove 343 may be different from each other, and the height H2 of the flow guiding groove 342 and the maximum height H4 of the flow guiding groove 343 may also be different from each other, which is not limited in the embodiments of the present disclosure.

It should be noted that, in the above embodiments shown in fig. 11-13C, the flow guiding grooves surrounding the detection groove 341 include flow guiding grooves 342 and flow guiding grooves 343 with different structures from each other, while in other embodiments of the present disclosure, the flow guiding grooves surrounding the detection groove 341 may also all have the same structure, for example, all are set as flow guiding grooves 342, or all are set as flow guiding grooves 343, which is not limited by the embodiments of the present disclosure.

In the above embodiments shown in fig. 11 to 13C, the diversion grooves are formed around the diamond-shaped detection groove, and the structure of the diversion groove is described. In some other embodiments of the present disclosure, when the detection groove is in other regular shapes or irregular shapes, such as triangle, circle, etc., a diversion groove may also be formed on the periphery of the corresponding detection groove, for example, a diversion groove may be formed on the periphery of the detection groove 141 of the detection chip 10 shown in fig. 1A-2B, which is not limited in this respect.

For example, in the embodiment of the present disclosure, the

flow guiding grooves

342 and 343 surround the detection groove 341, and the profile shape of the

flow guiding grooves

342 and 343 is the same as that of the detection groove 341, while in some other embodiments of the present disclosure, the profile shape of the

flow guiding grooves

342 and 343 may be different from that of the detection groove 341, and the embodiment of the present disclosure is not limited thereto.

For example, in some other embodiments of the present disclosure, the diversion groove may also partially surround the detection groove, for example, a diamond-shaped detection groove is taken as an example, and the diversion groove may be disposed only corresponding to two sides of the detection groove adjacent to the diversion groove, so as to achieve rapid mixing between the sample and the reaction membrane. Meanwhile, the detection result (such as color development) in a certain area or a certain range close to the center on the reaction membrane can be more obvious, so that the subsequent observation of the detection result is facilitated, and the accuracy of the obtained detection data is improved.

For example, as shown in fig. 12 and 13A, the diversion trench 330 extends into the detection groove 341, and the second end of the diversion trench 330, which is communicated with the detection groove 341, is located in the diamond-shaped detection groove 341, so that the sample can more rapidly and sufficiently infiltrate the reaction membrane 350, and the required detection time is shortened.

For example, the second end of the diversion trench 330 extends into the detection groove 341 by a length less than or equal to 1.5mm, such as 0.5mm or 1 mm. Therefore, the sample can be introduced into the detection groove 341 from the diversion trench 330, so that the sample can be fully immersed into the reaction film 350, the sample can fully react with the detection reagent in the reaction film 350, and the accuracy of the obtained detection data is improved.

For example, in some embodiments of the present disclosure, the reactive film 350 is in a compressed state in the thickness direction (i.e., in the direction of the height H1 shown in fig. 13A or 13B). For example, the thickness of the reaction film 350 in the relaxed state may be equal to or slightly greater than the height H1 of the detection groove 341, for example, the ratio of the thickness of the reaction film 350 in the relaxed state to the height H1 of the detection groove 341 may be 1:1-1:0.5, and after the first substrate 301 and the second substrate 302 are combined, the reaction film 350 is in close contact with the second substrate 302 due to the compression of the reaction film 350 along the thickness direction, thereby preventing the liquid storage phenomenon caused by the gap between the reaction film 350 and the first substrate 301 or the second substrate 302.

For example, as shown in fig. 11 and 12, the detecting part 340 may further include a liquid storage hole 380 penetrating through the first substrate 301 and communicating with the detecting groove 341, so that in the case that the amount of the sample injected through the sample injection opening 310 is excessive, the excessive sample may be stored in the liquid storage hole 380, thereby preventing or reducing a liquid leakage phenomenon caused by the excessive sample injection, and the liquid storage hole 380 may further promote the sample to infiltrate from the periphery to the center of the reaction membrane 350 through the

flow guide grooves

342 and 343, so that the detection result (e.g., color development) at the center of the reaction membrane 350 is more obvious.

For example, the diameter of the liquid storage through hole 380 may be set to 0.2mm-5mm, such as 2mm or 3mm, so as to be beneficial to controlling the sample amount, avoid or reduce the leakage caused by excessive sample injection, promote the sample to infiltrate from the periphery of the reaction film 350 to the center, and also reduce the processing technology and precision requirement of the liquid storage through hole 380, thereby simplifying the preparation technology.

For example, as shown in fig. 13B and 13C, the ratio of the depth H3 of the liquid storage through hole 380 to the height H1 of the detection groove 341 may be set to 0.5:1-10:1, such as 3:1, so as to perform the function of sample quantification, which is beneficial for controlling the sample amount, and at the same time, the liquid storage through hole 380 may store more samples, thereby preventing the samples from overflowing.

For example, the reservoir hole 380 communicates with the center of the detection recess 341, i.e., the reservoir hole 380 is opened at the center of the bottom surface of the detection recess 341, so as to communicate the center of the detection recess 341 with the external environment, e.g., the atmosphere, thereby better promoting the sample to infiltrate from the periphery to the center of the reaction membrane 350.

For example, in the manufacturing process of the detection chip 30, in order to facilitate the assembly of the detection chip 30, a negative pressure may be applied to the liquid storage through hole 380 during the assembly process to fix the reaction membrane 350 in the detection groove 341. Therefore, the sample can react with the detection reagent in the reaction film 350 more fully, and the accuracy of the obtained detection data is further improved.

For example, in one example of the present disclosure, the shape of the detection groove 341 is a diamond, two diagonal lines of the diamond are 8mm and 5.8mm, respectively, the height H1 of the detection groove 341 is 0.5mm, the difference between the height H2 of the flow guide groove 342 and the height H1 of the detection groove 341 is 0.3mm, the difference between the maximum height H4 of the flow guide groove 343 and the height H1 of the detection groove 341 is 0.3mm, the width D1 of the flow guide groove 342 is 0.3mm, the width D2 of the flow guide groove 343 is 0.3mm, the diameter of the liquid storage hole 380 is 2.5mm, and the depth H3 of the liquid storage hole 380 is 1 mm. Furthermore, the amount of the sample in the detection groove 341 can be controlled, the leakage caused by excessive sample injection can be avoided or reduced, the sample can be promoted to infiltrate from the periphery to the center of the reaction film 350, the amount of the sample infiltrating to the center of the reaction film 350 can be increased, the detection result (such as color development) of the center of the reaction film 350 can be more obvious, and the observation of the subsequent detection result can be facilitated.

It should be noted that, in some other embodiments of the present disclosure, the liquid storage through hole may also be formed at other positions on the bottom surface of the detection groove; alternatively, according to actual needs, a plurality of liquid storage through holes can be formed in each detection part. The setting position, the number and the like of the liquid storage through holes are not limited in the embodiment of the disclosure.

Fig. 14 is an exploded view, i.e., an exploded view, of another detecting chip according to at least one embodiment of the present disclosure. FIG. 14 is a plan view of the detection chip, showing the structure as viewed from the front of the detection chip. It should be noted that, except for the adhesive layer 704 and the sample-adding protrusion 790, the structure and function of the detecting chip 70 shown in fig. 14 are substantially the same as or similar to those of the detecting chip 30 shown in fig. 11, and are not repeated herein.

For example, fig. 14 shows a case where the detection chip 70 is bonded to the first substrate 701 and the second substrate 702 through the adhesive layer 704.

For example, as shown in fig. 14, an adhesive layer 704 is laminated between the first substrate 701 and the second substrate 702, that is, on the first surface of the first substrate 701.

For example, the adhesive layer 704 may be made of, for example, an adhesive material having hydrophobicity, such as a double-sided tape.

For example, as shown in fig. 14, the adhesive layer 704 includes an opening 7041 corresponding to the detection groove and the flow guide groove (not shown) of the first substrate 701, so that the obstruction of the sample flow due to the uneven surface and self-viscosity of the adhesive layer 704 can be avoided, and the adhesion between the adhesive layer 704 and the reaction film 750 can be avoided, thereby facilitating the flow of the sample in the detection groove and the flow guide groove, enabling the sample to quickly and sufficiently wet the reaction film 750, and improving the accuracy of the obtained detection result.

For example, in some embodiments of the present disclosure, the shape and size of the opening 7041 may be the same as the shape and size of the cross-section of the detection groove, thereby reducing or avoiding the obstruction of the sample flow by the adhesive layer 704, or reducing or avoiding the adhesion between the adhesive layer 704 and the reaction film 750. In some embodiments of the present disclosure, the shape of the opening 7041 may also be the same as the shape of the cross section of the detection groove, and the size of the opening 7041 is slightly smaller than the size of the cross section of the detection groove, so as to slightly increase the contact area between the adhesive layer 704 and the first substrate 701 and the second substrate 702, so as to improve the adhesion between the first substrate 701 and the second substrate 702, thereby further reducing or avoiding the sample from leaking out, and improving the sealing effect of the detection chip 70.

For example, a tip portion (e.g., a vertex portion) of the opening 7041 may be provided at a right angle, or may be provided as a rounded corner with a certain curvature, for example, a right angle type or a circular arc type. For example, taking the diamond-shaped opening 7041 in fig. 14 as an example, the four corners of the diamond shape may be set to be right angles or rounded with a certain arc, and the embodiment of the disclosure is not limited thereto.

For example, in some examples, taking the diamond-shaped opening 7041 in fig. 14 as an example, two sets of opposite corners of the diamond shape may be divided into a first set and a second set, and the first set of opposite corners may be set as right angles, and the second set of opposite corners may be set as circular arcs. For example, since the shape of the diamond-shaped opening 7041 is substantially the same as the outline shape of the detection groove of the first substrate 701, the size of the diamond-shaped opening 7041 can be slightly smaller than the size of the cross section of the detection groove by making the first set of opposite corners arc, so that the contact area between the adhesive layer 704 and the first substrate 701 and the second substrate 702 is increased to a certain extent, and the adhesion effect between the first substrate 701 and the second substrate 702 is improved; by providing the second set of opposing corners at right angles, the resistance of the adhesive layer 704 to the flow of the sample can be reduced or avoided to some extent, and the adhesion between the adhesive layer 704 and the reaction membrane 750 can be reduced or avoided.

Therefore, by providing the first set of opposite corners and the second set of opposite corners, problems such as the blocking of the adhesive layer 704 to the sample flow due to its own viscosity and the adhesion to the reaction membrane 750 can be reduced or avoided, and the bonding between the first substrate 701 and the second substrate 702 can be better achieved, thereby reducing or avoiding the leakage of the sample and improving the sealing effect of the detection chip 70.

It should be noted that in some other examples, all the top corners of the opening 7041 may be designed to be right angles, or all the top corners may be designed to be circular arcs or other suitable contours, which is not limited by the embodiments of the present disclosure.

It should be noted that, in the embodiment shown in fig. 14, the shape and size of the opening 7041 are substantially the same as the outline shape and size of the detection groove and the diversion groove of the first substrate 701, but in some other embodiments of the present disclosure, the size of the opening of the adhesive layer may be slightly larger or smaller than the size of the detection groove or the diversion groove, or may be set to other suitable shapes or sizes, as long as the blocking of the sample infiltration reaction film by the adhesive layer can be avoided or weakened and the sealing effect of the detection chip can be ensured, which is not limited by the embodiments of the present disclosure.

For example, as shown in fig. 14, compared to the detection chip 30 shown in fig. 11, the detection chip 70 employs the sample addition protrusion 790 having a petal-shaped cross section, so that the contact area between the sample addition protrusion 790 and the first substrate 701 can be increased, which is favorable for the stable connection between the sample addition protrusion 790 and the first substrate 701, and is more suitable for holding with one hand, fixing or picking with a device, and the like.

For example, as shown in fig. 14, the first substrate 701 includes a first notch 7011, the second substrate 702 includes a second notch 7021 corresponding to the first notch 7011, and the first notch 7011 and the second notch 7021 overlap with each other in a direction perpendicular to a surface of the first substrate 701 or the second substrate 702. The first notch 7011 and the second notch 7021 form a limiting portion (or referred to as a limiting block), so that the detection chip 70 can be fixed on, for example, a detection device through the limiting block formed by the first notch 7011 and the second notch 7021, thereby facilitating detection of the reaction film 750 in the detection chip 70 and improving accuracy of the obtained detection data. In addition, a plurality of detection branch structures in the detection chip 70 can be marked and positioned by the positions of the first notch 7011 and the second notch 7021, thereby facilitating, for example, observation and recording of detection data.

It should be noted that the sizes of the first concave 7011 and the second concave 7021 may be determined according to the structure of, for example, the detection device to which the first concave 7011 and the second concave 7021 are fixed, and the embodiment of the present disclosure is not limited thereto as long as the detection chip 70 can be fixed to the detection device through the first concave 7011 and the second concave 7021.

For example, the first and

second recesses

7011 and 7021 may be disposed between adjacent detection grooves in a direction perpendicular to the surface of the first or

second substrate

701 or 702, or other suitable positions, which embodiments of the present disclosure do not limit.

For example, in the case where the detection chip 70 includes the adhesive layer 704 or other structural or functional layer, the structural or functional layer also has a notch structure at a position corresponding to the first notch 7011 and the second notch 7021, so that the detection chip 70 integrally forms a stopper.

For example, in some embodiments of the present disclosure, the sample adding opening, the guiding gutter, the detection groove, and other structures of the detection chip may also be set in other manners, as long as the arrangement of the sample adding opening, the guiding gutter, the detection groove, and other structures can be realized and the corresponding functions can be realized. The above embodiment is described in the case that the sample adding opening penetrates through the first substrate and the structures such as the diversion trench and the detection groove are disposed on the first surface of the first substrate of the detection chip, but this does not limit the disclosure.

Other possible arrangement positions of the diversion trench and the detection groove are exemplarily described below on the basis of the detection chip 30 shown in fig. 11.

For example, fig. 15A and 15B are exploded views, i.e., exploded views, of another detection chip provided in at least one embodiment of the present disclosure. For example, fig. 15A is a top view of the detection chip, and fig. 15B is a bottom view of the detection chip. It should be noted that, for the structures, functions, and the like of specific components in the detection chip 40 shown in fig. 15A and 15B, reference may be made to the corresponding descriptions of the detection chip 10, the detection chip 20, or the detection chip 30 in the foregoing embodiments, and no further description is provided here.

For example, as shown in fig. 15A and 15B, the detection chip 40 includes a first substrate 401, a second substrate 402, and a third substrate 403.

For example, first substrate 401 has a first surface (shown as an upper surface) and a second surface (shown as a lower surface) opposite to each other, channel 430 is formed on the second surface of first substrate 401, and sensing groove 441 is formed on the first surface of first substrate 401. The second substrate 402 is laminated on the first surface of the first substrate 401 and has a detection through-hole 460 at a position corresponding to the detection groove 441 to allow optical detection. Third substrate 403 is stacked on the second surface of first substrate 401 to seal flow guide channels 430 and sample application opening 410, so that the sample can flow into flow guide channels 430 from sample application opening 410.

For example, the sample loading opening 410 is a through hole penetrating through the first substrate 401, the second substrate 402 has a through hole exposing the sample loading opening 410, so that the sample can be loaded into the sample loading opening 410, and the sample loading protrusion 490 can extend from the second substrate 402, so as to facilitate taking and placing of the detection chip 40, for example.

For example, fig. 16 is an exploded view, i.e., an exploded view, of another detection chip provided in at least one embodiment of the present disclosure. For example, fig. 16 is a top view of a detection chip. It should be noted that, for the structure, the function, and the like of the specific components in the detection chip 50 shown in fig. 16, reference may be made to the corresponding description of the detection chip 10, the detection chip 20, or the detection chip 30 in the foregoing embodiments, and no further description is given here.

For example, as shown in fig. 16, the detection chip 50 includes a first substrate 501 and a second substrate 502. The first substrate 501 has a first surface (shown as an upper surface) and a second surface (shown as a lower surface) opposite to each other, the guide groove 530 and the detection groove 541 are formed on the first surface of the first substrate 501, and the second substrate 502 is stacked on the first surface of the first substrate 501 and has a detection through-hole 560 at a position corresponding to the detection groove 541 to allow optical detection.

For example, the sample-adding opening 510 is an opening in the first substrate 501, such as a non-through hole that does not penetrate through the first substrate 501, and at this time, the height (or depth) of the sample-adding opening 510 is smaller than the thickness of the first substrate 501. The second substrate 502 has a through hole exposing the sample loading opening 510 and a sample loading protrusion 590, so that the sample can be injected into the sample loading opening 510 through the sample loading protrusion 590, and the detection chip 50 can be conveniently taken and placed, for example.

At least one embodiment of the present disclosure also provides a detection system, including: the detection device and the detection chip provided by any embodiment of the present disclosure, such as the detection chip 10, the detection chip 20, the detection chip 30, the detection chip 40, or the detection chip 50 in the above embodiments, are configured to detect the reaction film in the detection groove through the detection portion of the detection chip.

For example, taking the detection chip 10 in the above embodiments as an example, fig. 17 is a schematic diagram of a detection system provided in at least one embodiment of the present disclosure.

For example, as shown in fig. 17, the detecting system 60 includes a detecting chip 10 and a detecting device 610, and the detecting device 610 is configured to detect the reactive agent in the detecting groove 141, such as the reactive film 150, by the detecting part 140 of the detecting chip 10.

For example, the detection device 610 includes a light source 611 and a photodetection device 612. The light source 611 is configured to emit light toward the reaction film 150, and the photodetection device 612 is configured to receive the light emitted from the light source 611 and reflected by the reaction film 150.

For example, the photodetection device 612 can compare the intensity of the light reflected by the reaction film 150 with the intensity of the light emitted by the light source 611, and determine the presence or absence, concentration, and the like of the sample according to the value of the absorbance of the reaction film 150 to be detected, thereby detecting the sample index. For example, taking a detection reagent titrated in advance on the reaction film 150 as a color reagent as an example, when the detection result is obtained, the darker the color developed on the reaction film 150 indicates that the content of the analyte in the sample to be detected is higher, and accordingly, the value of the absorbance of the reaction film 150 detected by the photoelectric detection device 612 is higher.

For example, in some embodiments, the photo detection device 612 may be a photodiode, which can convert the received light signal into an electrical signal, and then determine the intensity of the received light according to the change of the electrical parameter (e.g., the change of the current, etc.) in the electrical signal, so as to determine the value of the absorbance of the reaction membrane 150.

For specific description and technical effects of the detection system provided in the embodiment of the present disclosure, reference may be made to corresponding contents in the detection chip provided in the embodiment of the present disclosure, for example, reference may be made to corresponding contents of the detection chip 10, the detection chip 20, the detection chip 30, the detection chip 40, or the detection chip 50 in the above embodiments, and details are not repeated herein.

The following points need to be explained:

(1) the drawings of the embodiments of the present disclosure relate only to the structures related to the embodiments of the present disclosure, and other structures may refer to general designs.

(2) For purposes of clarity, the thickness of layers or regions in the figures used to describe embodiments of the present disclosure are exaggerated or reduced, i.e., the figures are not drawn on a true scale. It will be understood that when an element such as a layer, film, region, or first substrate is referred to as being "on" or "under" another element, it can be "directly on" or "under" the other element or intervening elements may be present.

(3) Without conflict, embodiments of the present disclosure and features of the embodiments may be combined with each other to arrive at new embodiments.

The above is only a specific embodiment of the present disclosure, but the scope of the present disclosure is not limited thereto, and the scope of the present disclosure should be determined by the scope of the claims.

Claims

1. A detection chip, comprising:

the sample-adding opening is provided with a sample-adding opening,

at least one detection branch structure, wherein each of the at least one detection branch structure comprises:

a detection part comprising a detection groove and a reaction reagent,

wherein the detection groove is communicated with the sample addition opening, the reaction reagent is accommodated in the detection groove,

the detection part is configured to allow optical detection of the reaction reagent located in the detection groove.

2. The detection chip of claim 1, wherein each of the at least one detection branch structure further comprises a flow guide trench,

the flow guide groove is provided with a first end and a second end, the first end of the flow guide groove is communicated with the sample adding opening, and the second end of the flow guide groove is communicated with the detection groove, so that the detection groove is communicated with the sample adding opening through the flow guide groove.

3. The detection chip of claim 2, further comprising:

the sample feeding opening is a through hole in the first substrate, and the diversion trench and the detection groove are formed on the first surface of the first substrate;

a second substrate laminated on the first surface of the first substrate and allowing the optical detection at a position corresponding to the detection groove.

4. The detection chip of claim 3, wherein each of the at least one detection branch structures further comprises a water-absorbing film,

the water absorbing film is accommodated in the detection groove and is at least partially stacked with the reaction reagent in a direction perpendicular to the first substrate.

5. The detection chip according to claim 4, wherein the water-absorbing film is provided on a side of the reaction reagent remote from the second substrate.

6. The detection chip according to claim 4 or 5, wherein the water-absorbent film has a liquid storage amount of 10 μ L to 50 μ L.

7. The detecting chip according to any one of claims 2 to 5, wherein the detecting portion further comprises a flow guide groove located at the periphery of the detecting groove, the flow guide groove being in communication with the detecting groove;

at least part of the diversion grooves are smaller than the detection grooves in height.

8. The detecting chip of claim 7, wherein the flow guiding recess includes a flow guiding wall having a slope shape,

one end of the flow guide wall is connected with the side surface of the detection groove.

9. The detecting chip according to claim 7, wherein the flow guiding groove is stepped integrally with a longitudinal section of the detecting groove.

10. The detection chip of claim 7, wherein the flow guide groove at least partially surrounds the detection groove.

11. The detection chip of claim 7, wherein the height of the detection groove is 0.2mm to 5mm, the difference between the maximum height of the flow guide groove and the height of the detection groove is 0.1mm to 1mm, and the width of the flow guide groove is 0.1mm to 1 mm.

12. The detecting chip according to any one of claims 3 to 5, wherein the detecting portion further includes a liquid storage through hole penetrating the first substrate and communicating with the detecting recess.

13. The detection chip according to claim 12, wherein the liquid storage through hole communicates with the center of the detection groove.

14. The detection chip according to claim 12, wherein the diameter of the liquid storage through hole is 0.2mm-5mm, and the ratio of the depth of the liquid storage through hole to the height of the detection groove is 0.5:1-10: 1.

15. The detecting chip according to any one of claims 2 to 5, wherein the height of the flow guide groove is 0.1mm to 1.5mm, and the width of the flow guide groove is 0.1mm to 2 mm.

16. The detection chip of any one of claims 2 to 5, wherein the ratio of the height of the flow guide groove to the width of the flow guide groove is 1:1 to 10: 1.

17. The detecting chip according to any one of claims 2 to 5, wherein the inner wall of the flow guide groove has hydrophilicity.

18. The detection chip according to any one of claims 2 to 5, wherein the at least one detection branch structure comprises a plurality of detection branch structures, and the plurality of detection branch structures are uniformly distributed along the periphery of the sample addition opening.

19. The detecting chip of any one of claims 2-5, wherein the sample loading opening comprises a first body and a first protrusion protruding from the first body toward the flow guide groove, and the first protrusion is in communication with the flow guide groove.

20. The detection chip of claim 19, wherein the first body has a diameter of 1mm-10 mm.

21. The detection chip according to any one of claims 2 to 5, wherein the reaction reagent comprises a reaction membrane and/or a bulk reaction reagent.

22. The detection chip according to claim 21, wherein the reaction membrane is in a compressed state in a thickness direction.

23. The detection chip of claim 22, wherein a ratio of a thickness of the reaction film in a relaxed state to a height of the detection groove is 1:1 to 1: 0.5.

24. The detection chip according to claim 21, wherein the reaction membrane is circular, or,

the reaction membrane is polygonal, and one corner of the polygon is directly communicated with the second end of the diversion trench.

25. The detection chip of claim 21, wherein the reaction membrane comprises a membrane main body and a second protrusion protruding from the membrane main body toward the flow guide groove, at least a portion of the second protrusion being located in the flow guide groove;

the membrane main body is in the same shape as the detection groove and is circular.

26. The detection chip of claim 21, wherein the reaction membrane has a diamond shape.

27. The detection chip according to claim 24, wherein the diameter of the detection groove is 3mm to 15mm, and the diameter of the reaction membrane is equal to or smaller than the diameter of the detection groove.

28. The detection chip according to any one of claims 3 to 5, wherein the second substrate has a detection through-hole at a position corresponding to the detection groove.

29. The detection chip of claim 28, wherein the second substrate is opaque to light; or,

the detection chip further comprises a light shielding layer, wherein the light shielding layer covers the surface of the second substrate far away from and/or close to the first substrate, and exposes the detection through hole.

30. The detection chip of claim 28, wherein the diameter of the detection through hole is 2mm-10 mm.

31. The detection chip of any one of claims 3 to 5, wherein the material of the first substrate comprises one or more of polymethyl methacrylate, polystyrene and polycarbonate.

32. The detection chip of any one of claims 3 to 5, wherein the material of the second substrate comprises one or more of polymethyl methacrylate, polystyrene, polycarbonate and polyethylene terephthalate.

33. The detection chip according to any one of claims 3 to 5, wherein the first substrate and the second substrate are bonded by bonding, welding, adhering or clamping.

34. The detection chip of claim 33, further comprising an adhesive layer;

wherein the adhesive layer is located between the first substrate and the second substrate and is used to bond the first substrate and the second substrate,

the adhesive layer includes an opening corresponding to the detection groove.

35. The detection chip according to any one of claims 2 to 5, wherein the reaction reagent comprises a base material and a detection reagent distributed in the base material,

the matrix material comprises glass fibers, cotton fibers or composite fibers of the glass fibers and the cotton fibers.

36. The detection chip of claim 2, further comprising:

a second substrate laminated on the first surface of the first substrate and having a detection through-hole at a position corresponding to the detection groove to allow the optical detection through the detection through-hole;

the at least one detection branch structure comprises a plurality of detection branch structures which are uniformly distributed along the periphery of the sample adding opening.

37. The detection chip of claim 2, further comprising:

the sample feeding opening is a through hole in the first substrate, the detection groove is formed on the first surface of the first substrate, and the flow guide groove is formed on the second surface of the first substrate;

a second substrate that is laminated on the first surface of the first substrate and allows the optical detection at a position corresponding to the detection groove;

and a third substrate stacked on the second surface of the first substrate and sealing the sample injection opening and the guide groove.

38. The detection chip of claim 2, further comprising:

the sample feeding opening, the flow guide groove and the detection groove are formed on the first surface of the first substrate, and the sample feeding opening is a non-through hole in the first substrate;

a second substrate laminated on the first surface of the first substrate and exposing the sample addition opening and allowing the optical detection at a position corresponding to the detection groove.

39. The detection chip according to any of claims 2 to 5, further comprising an optical alignment branching structure,

wherein the optical calibration branching structure comprises an optical path detection area configured to perform optical calibration.

40. The detection chip according to any one of claims 3 to 5, further comprising a sample addition projection part:

wherein the sample addition convex part protrudes from the first surface of the first substrate along a direction far away from the second substrate,

one end of the sample adding bulge is connected with the sample adding opening.

41. The detection chip of claim 40, wherein the sample application projection comprises a conical cavity,

the volume of the conical cavity is 50-200 mu L, and the height of the conical cavity protruding out of the first substrate is 1-20 mm;

the conical cavity comprises a first end and a second end which are opposite to each other, the first end is connected with the sample adding opening, the diameter of the first end is 0.5mm-5mm, and the diameter of the second end is 1mm-20 mm.

42. The detection chip of any one of claims 3 to 5, wherein the first substrate comprises a first notch, the second substrate comprises a second notch corresponding to the first notch,

the first notch and the second notch are used to fix the detection chip.

43. A detection system, comprising:

the detection chip according to any one of claims 1 to 42,

a detection device configured to detect the reaction reagent in the detection groove by the detection part.

44. The detection system of claim 43, wherein the detection device comprises:

a light source configured to emit light toward the reaction reagent,

a photodetection device configured to receive light emitted from the light source and reflected by the reactive agent.