CN113945550A - Drug detection method and device based on fluorescent colloidal gold - Google Patents

Drug detection method and device based on fluorescent colloidal gold Download PDF

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CN113945550A
CN113945550A CN202111215888.7A CN202111215888A CN113945550A CN 113945550 A CN113945550 A CN 113945550A CN 202111215888 A CN202111215888 A CN 202111215888A CN 113945550 A CN113945550 A CN 113945550A
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colloidal gold
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阮系真
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Hangzhou Zhenxi Biotechnology Co ltd
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Hangzhou Zhenxi Biotechnology Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/558Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587Nanoparticles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors

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Abstract

The invention discloses a method and a device for detecting drugs based on fluorescent colloidal gold, wherein the method comprises the following steps: the mobile terminal establishes wireless communication connection with the fluorescent colloidal gold two-in-one detection device in a two-dimensional code mode of the scanning device; inputting personnel information of a virus detection object; scanning the unique code of the reagent card board through the mobile terminal; the mobile terminal binds the scanned card board coding information with the personnel information of the virus detection object; pushing the reagent card board carrying the toxicity test sample into a detection port of the equipment; sending a virus detection instruction to the two-in-one detection device through the mobile terminal; and after receiving the virus detection instruction, the two-in-one detection equipment performs card reading comparison on the reagent card board to form a virus detection result and pushes the virus detection result to the mobile terminal. The invention controls the virus detection process of the two-in-one detection device through the mobile terminal, improves the intelligent level of the device virus detection, binds the unique code of the reagent card board with the information of the personnel of the virus detection object, and effectively prevents the virus detection from being false and wrong and leaked.

Description

Drug detection method and device based on fluorescent colloidal gold
Technical Field
The invention relates to the technical field of drug detection, in particular to a method and a device for detecting drugs based on fluorescent colloidal gold.
Background
The immune colloidal gold technology and the fluorescence immunochromatography technology are widely applied to the field of drug detection due to the advantages of low detection cost, convenient use, rapid result reading, high detection sensitivity and the like. However, the detection principles of colloidal gold and fluorescence are not consistent, and most of the existing toxicity detection mechanisms use two different special devices to perform colloidal gold or fluorescence detection on drug samples, so that the use cost is high, and the convenience is poor.
Although two unification check out test set have appeared in the market at present, detect the function integration with fluorescence detection and colloidal gold and be in the same place, though solved and used convenience and problem with high costs, but current two unification check out test set includes detection mechanism and control mechanism, and inner structure is complicated, and is bulky, the portability is relatively poor, intelligent degree is lower, the detection record is not traceable, and most crucial, current two unification check out test set can't bind reagent card and poison object personnel information mutually, appear easily that detection such as false detection and detection mistake are leaked such as false poison detection.
Disclosure of Invention
The invention provides a method and a device for detecting drugs based on fluorescent colloidal gold, aiming at improving the intelligent degree of fluorescent colloidal gold two-in-one detection equipment, simplifying the internal structure of the two-in-one equipment, improving the use portability of the equipment, preventing false detection and wrong and missed detection.
In order to achieve the purpose, the invention adopts the following technical scheme:
the drug detection method based on the fluorescent colloidal gold comprises the following steps:
step S1, starting the fluorescent colloidal gold two-in-one detection device;
step S2, the mobile terminal establishes wireless communication connection with the fluorescent colloidal gold two-in-one detection device in a scanning device two-dimensional code mode;
step S3, inputting the personnel information of the virus detection object;
step S4, scanning the unique code of the fluorescent reagent card or the colloidal gold reagent card through the mobile terminal;
step S5, the mobile terminal binds the scanned card board coding information and the personnel information of the virus detection object;
step S6, pushing the fluorescent reagent card board or the colloidal gold reagent card board loaded with the toxicity test sample into a device detection port;
step S7, a poison detection instruction is sent to the fluorescent colloidal gold two-in-one detection device through the mobile terminal;
step S8, after receiving the virus detection instruction, the two-in-one fluorescent colloidal gold detection equipment reads the card of the reagent card and compares the card to form a virus detection result related to the virus detection object and pushes the virus detection result to the mobile terminal;
and step S9, the mobile terminal forms the virus detection result into a virus detection record and stores the virus detection record.
Preferably, in step S4, after the unique code of the fluorescent reagent card or the colloidal gold reagent card is scanned, the mobile terminal queries whether the currently scanned unique code of the card has an over-binding record from the card code binding record library,
if so, prompting a detector that the current card board has a binding record and refusing to bind again;
if not, the process proceeds to step S5.
Preferably, the fluorescent reagent card board or the colloidal gold reagent card board printed with the unique code is escorted to a drug detection site through a smart material evidence box, and the method for intelligently escorting the fluorescent reagent card board or the colloidal gold reagent card board comprises the following steps:
l1, scanning the two-dimensional codes of the intelligent material evidence box through the mobile terminal to establish mutual wireless communication connection;
step L2, after the fluorescent reagent card board and/or the colloidal gold reagent card board are/is placed in the intelligent material evidence box and a box cover is covered, a box opening password is set through the mobile terminal, and then a box locking instruction is sent to the intelligent material evidence box;
step L3, after the controller of the intelligent material evidence box receives the box locking instruction, controlling a box locking mechanism to execute a box locking action so as to lock the intelligent material evidence box;
step L4, after the intelligent material evidence box is escorted to a drug detection site, a detection person inputs the box opening password set in the step L2 through the mobile terminal and sends a box opening instruction to the intelligent material evidence box;
and L5, after receiving the unpacking command, the controller of the intelligent material evidence box controls an unpacking mechanism to execute unpacking actions so as to open the intelligent material evidence box.
Preferably, the unique code printed on the fluorescent reagent card or the colloidal gold reagent card contains the required virus detection item information, and the mobile terminal automatically acquires the virus detection item corresponding to the sample from the cloud after scanning and identifying the virus detection item information contained in the unique code of the card and writes the virus detection item into the fluorescent colloidal gold two-in-one detection device.
Preferably, the unique code printed on the fluorescent reagent card or the colloidal gold reagent card contains the required virus detection label information, and the mobile terminal automatically acquires the label data corresponding to the virus detection project from the cloud and writes the label data into the fluorescent colloidal gold two-in-one detection device after scanning and identifying the virus detection label information contained in the unique code of the card.
Preferably, in step S2, after the device two-dimensional code of the fluorescent colloidal gold two-in-one detection device is scanned, the mobile terminal actively acquires the device code information of each device having the operation authority of the current login account from the database and compares the device code information with the currently scanned two-in-one device code information,
if the comparison is successful, establishing wireless communication connection with the two-in-one detection device;
if the comparison fails, the wireless communication connection with the two-in-one detection device is refused to be established.
The invention also provides a drug detection device based on the fluorescent colloidal gold, which can realize the drug detection method, the drug detection device comprises a mobile terminal and fluorescent colloidal gold two-in-one detection equipment, and the mobile terminal comprises:
the code scanning module is used for providing a detection person to call a terminal camera to scan the two-dimensional code of the fluorescent colloidal gold two-in-one detection device, or the unique code of the fluorescent reagent card board, or the unique code of the colloidal gold reagent card board;
the communication connection module is connected with the code scanning module and used for establishing wireless communication connection with the fluorescent colloidal gold two-in-one device after scanning the two-dimensional code of the device;
the virus detection object personnel information input module is used for providing the personnel for the detection to input personnel information of the virus detection object;
the information binding module is connected with the code scanning module and the personnel information input module of the virus detection object and is used for carrying out information binding on the scanned card board coding information and the personnel information of the virus detection object and storing the binding information into a card board coding binding database;
the virus detection instruction generating and sending module is used for providing the virus detection instruction for the detection personnel to generate and send the virus detection instruction to the fluorescent colloidal gold two-in-one equipment;
and after the fluorescent colloidal gold two-in-one detection equipment receives the virus detection instruction and then performs card reading comparison on the reagent card, forming a virus detection result related to the virus detection object and pushing the virus detection result to the mobile terminal.
Preferably, the mobile terminal further includes:
a reagent card binding record inquiry module which is connected with the code scanning module and is used for inquiring whether the currently scanned unique code of the fluorescent reagent card or the colloidal gold reagent card has binding record or not from a card code binding database after the unique code of the fluorescent reagent card or the colloidal gold reagent card is scanned,
if yes, generating prompt information that the current card board cannot be bound and displaying the prompt information to the detection personnel;
if not, no response is made;
the virus inspection item cloud acquisition module is connected with the code scanning module and is used for automatically acquiring a virus inspection item to be detected from the cloud after scanning and identifying required virus inspection item information contained in the unique code of the card;
the cloud acquisition module for the virus inspection label data is connected with the code scanning module and is used for automatically acquiring label data corresponding to the virus inspection item from the cloud after the virus inspection label data information contained in the unique code of the card is scanned and identified;
and the data writing module is connected with the virus detection project cloud acquisition module and the virus detection standard curve data cloud acquisition module and is used for writing the acquired virus detection project and standard curve data into the fluorescent colloidal gold two-in-one detection device.
Preferably, drug detection device is still including being used for escort fluorescence reagent cardboard and/or the intelligent material evidence case of colloidal gold reagent cardboard, in the mobile terminal sweep the yard module and still be used for sweeping the equipment two-dimensional code of intelligent material evidence case, communication connection module still be used for sweep realize behind the equipment two-dimensional code with the wireless communication of intelligent material evidence case is connected, still include in the mobile terminal:
the unpacking password setting module is used for providing the escort personnel with the unpacking passwords of the intelligent material evidence box and storing the unpacking passwords;
the box locking instruction generating and sending module is connected with the box opening password setting module and used for automatically generating a box locking instruction after the box opening password is set and sending the box locking instruction to the intelligent material evidence box;
the box opening password input module is used for providing the box opening person with the box opening password to input the box opening password;
an unpacking password verification module connected with the unpacking password input module and used for comparing the input unpacking password with the stored unpacking password,
if the comparison is successful, generating a box opening instruction and sending the box opening instruction to the intelligent material evidence box;
if the comparison fails, generating a password error prompt message to display the unpacking personnel;
include in the intelligence material evidence case:
the instruction receiving module is used for receiving the box locking instruction or the box opening instruction sent by the mobile terminal;
and the control module is connected with the instruction receiving module and used for receiving the locking box instruction to control the locking box mechanism to execute the locking box action so as to lock the intelligent material evidence box or receiving the unlocking instruction to control the unlocking mechanism to execute the unlocking action so as to open the intelligent material evidence box.
The invention has the following beneficial effects:
1. the intelligent level of the two-in-one detection equipment is improved by controlling the poison detection process of the fluorescent colloidal gold two-in-one detection equipment through the mobile terminal, and the poison detection control function of the two-in-one detection equipment is transferred to the mobile terminal, so that a control mechanism originally integrated in the two-in-one equipment can be removed, the internal structure of the equipment is simplified, the size of the equipment is reduced, the convenience of the use of the equipment is improved, and the manufacturing cost of the equipment is reduced;
2. the unique code is printed on each reagent card, and the personnel information of the virus detection object is bound with the unique code information of the card, so that the phenomena of false detection of false name and false detection and missed detection of wrong detection are effectively avoided, and the virus detection flow is standardized.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments of the present invention will be briefly described below. It is obvious that the drawings described below are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.
FIG. 1 is a diagram illustrating steps of a method for detecting drugs based on colloidal gold fluorescence according to an embodiment of the present invention;
FIG. 2 is a diagram of the method steps for intelligent escort of reagent cards;
FIG. 3 is a schematic interface diagram of an APP software application code scanning connection fluorescent colloidal gold two-in-one detection device running on a mobile terminal;
FIG. 4 is a schematic interface diagram of a fluorescence colloidal gold two-in-one detection device connected to APP software application communication;
FIG. 5 is a diagram of a display interface of a virus detection result on an APP software application;
FIG. 6 is a schematic diagram of an interface of the APP software application code scanning connection intelligent material evidence box;
FIG. 7 is a schematic diagram of an interface for setting an open box password for a smart material evidence box;
FIG. 8 is a schematic diagram of an interface for entering an open box password for a smart material evidence box;
FIG. 9 is a schematic structural diagram of a device for detecting drugs based on colloidal gold fluorescence according to an embodiment of the present invention;
fig. 10 is an internal structure diagram of the mobile terminal;
fig. 11 is an internal structure schematic diagram of the intelligent material evidence box.
Detailed Description
The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.
Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "inner", "outer", etc. are used for indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not indicated or implied that the referred device or element must have a specific orientation, be constructed in a specific orientation and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limitations of the present patent, and the specific meanings of the terms may be understood by those skilled in the art according to specific situations.
In the description of the present invention, unless otherwise explicitly specified or limited, the term "connected" or the like, if appearing to indicate a connection relationship between the components, is to be understood broadly, for example, as being fixed or detachable or integral; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or may be connected through one or more other components or may be in an interactive relationship with one another. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
An embodiment of the present invention provides a method for detecting a drug based on a fluorescent colloidal gold, as shown in fig. 1, including the following steps:
step S1, starting the fluorescent colloidal gold two-in-one detection device;
step S2, establishing wireless communication connection between a mobile terminal (such as a mobile phone) and a fluorescent colloidal gold two-in-one detection device in a two-dimensional code mode of a scanning device; specifically, as shown in fig. 3, a fluorescent colloidal gold poison detection APP software application is run on the mobile terminal, the detection personnel calls a camera of the mobile terminal to scan the unique device two-dimensional code printed on the side surface of the two-in-one detection device by clicking a 'scan connection detector' in the interface shown in fig. 3,
in order to prevent the two-in-one detection device from being illegally used by non-detection personnel, preferably, after the two-in-one device two-dimensional code is scanned, the APP software application actively compares whether the current login account has the authority to operate the current code scanning device, if the current login account does not have the operation authority, the APP software application refuses to establish communication connection with the two-in-one detection device, and if the current login account has the operation authority, the APP software application establishes wireless communication connection with the two-in-one detection device. The permission comparison mode is that after the device code information of the device with permission operation of each login account is stored in the database, and the device two-dimensional code (containing the device code information) of the two-in-one detection device is scanned, the APP software application actively inquires the device code information with permission operation of the current login account from the database and compares the information with the currently scanned device code information, if the comparison is successful, the current login account is indicated to have the operation permission, otherwise, no operation permission exists. Referring to fig. 4, if the word "connected" (for example, "detector [ new _ V5.24] connected" in fig. 4) appears in fig. 4, it represents that the APP software application and the two-in-one detection device have established the wireless communication connection.
Step S3, inputting the personnel information of the virus detection object, including the name, identification card number, sex, contact way, address and other information of the virus detection object, the input way is: expanding a 'personnel information' column in the figure 4, filling personnel information into a corresponding information frame and storing the personnel information;
step S4, scanning the unique code of the fluorescent reagent card or the colloidal gold reagent card through the mobile terminal; specifically, the inspector calls the camera of the mobile terminal by clicking a 'scan bar code' button in the interface of fig. 4 to scan the unique code printed on the reagent card;
step S5, the mobile terminal binds the scanned card board coding information and the personnel information of the virus detection object so as to avoid the possible error phenomena of corresponding error of the reagent card board and the virus detection object, undetected detection and the like in the virus detection work; for example, the coded information of the scanned reagent card is "a 000", the expression form of the human information is "wanghua-330102 xxxxxxxxxxxx", "wanghua" represents the name, "330102 xxxxxxxxxxxxxxxx" is the identification number of "wanghua", and the expression form after the binding of the coded information of the reagent card and the human information may be "a 000-wanghua-330102 xxxxxxxxxxxx".
In the virus detection work, the virus detection of the reagent card plate which is detected as negative by part of the detectors can be bound to the virus detector again to be false. In order to prevent this, after the unique code of the fluorescent reagent card or the colloidal gold reagent card is scanned in step S4, the mobile terminal queries whether the scanned unique code of the card has an over-binding record from the card code binding record library,
if so, prompting a detector that the current card board has a binding record and refusing to bind again;
if not, the process proceeds to step S5.
Step S6, pushing the fluorescent reagent card board or the colloidal gold reagent card board loaded with the toxicity test sample into an equipment detection port of the fluorescent colloidal gold two-in-one detection equipment;
step S7, a virus detection instruction is sent to the fluorescent colloidal gold two-in-one detection device through the mobile terminal; specifically, a virus detection instruction is sent to the two-in-one detection device by clicking a 'start detection' button in the interface of fig. 4;
step S8, after receiving the virus detection instruction, the two-in-one fluorescent colloidal gold detection device performs card reading comparison on the reagent card, and pushes the virus detection result of the formed associated virus detection object to the APP software application, the APP software application displays the virus detection result to the detection personnel through the interface shown in fig. 5, and the APP software application forms the virus detection result into a virus detection record and stores the virus detection record;
in step S9, the mobile terminal forms the poison test result into a poison test record and stores the poison test record.
The uniqueness of the reagent card board code and the information binding of the reagent card board unique code and the personnel information of the virus detection object are the keys of solving the false and wrong detection of the virus detection, and refusing to bind the reagent card board unique code with the binding record again. Specifically, as shown in fig. 2, the method for intelligently escorting a reagent card includes:
and L1, scanning the two-dimensional codes of the intelligent material evidence box through the mobile terminal to establish mutual wireless communication connection. Specifically, as shown in fig. 6, the escort personnel click a "connect to the physical evidence box" button in the software interface shown in fig. 6 to call a camera of the mobile terminal to scan the two-dimensional code of the device printed on the intelligent physical evidence box;
step L2, placing the fluorescent reagent card board and/or the colloidal gold reagent card board into the intelligent material evidence box and covering the box cover, and sending a box locking instruction to the intelligent material evidence box after setting a box opening password through the mobile terminal; the method for setting the unpacking passwords is shown in fig. 7, wherein an escort person clicks a "locking and escorting" button in a software interface shown in fig. 7, inputs two unpacking passwords at a skipped position where the escorting passwords are input, namely, the process of setting the unpacking passwords is completed, and a box locking instruction is sent to the intelligent material evidence box, and when the unpacking passwords input for the second time are not consistent with the unpacking passwords input for the first time, the unpacking passwords are required to be reset after the setting of the unpacking passwords fails;
step L3, after the controller of the intelligent material evidence box receives the box locking command, the controller controls the box locking mechanism to execute the box locking action to lock the intelligent material evidence box;
l4, after the intelligent physical evidence box is escorted to a drug detection site, a detection person inputs the box opening password set in the L2 through the mobile terminal and sends a box opening instruction to a controller of the intelligent physical evidence box; referring to fig. 8, a detection person clicks a "password unpacking" button in fig. 8, and after a set unpacking password is input in the previous unpacking password input interface, the APP sends an unpacking instruction to the intelligent material evidence box (if the password is input incorrectly, the APP generates "unpacking password error" information to prompt the unpacking person);
and L5, after receiving the unpacking command, the controller of the intelligent material evidence box controls the unpacking mechanism to execute unpacking action so as to open the intelligent material evidence box.
In the prior art, a fluorescent virus detection device or a colloidal gold virus detection device is generally provided with an item slot and a standard curve slot, wherein the item slot is used for inserting a virus detection item card, and a virus detection item to be detected is stored in the virus detection item card; the identification card slot is used for inserting an identification card, identification data of a toxic test item is stored in the identification card, the toxic test equipment reads the toxic test item in the item card during toxic test, carries out toxic test on the sample according to the read item, reads the identification data in the identification card, compares the toxic test data with the corresponding identification data and then makes a toxic test result. The existing project card and mark card reading mode has the following defects:
1. a project clamping groove and a mark curve clamping groove are required to be arranged in the virus detection equipment, so that the structural complexity of the equipment is increased;
2. the virus detection items and the label data of different virus detection samples are usually different, and the corresponding virus detection item card and the corresponding label card need to be selected to be inserted into equipment according to the sample types during virus detection, so that the method is very troublesome and is easy to make mistakes.
In order to solve the above 2 defects, the present invention adopts a technical scheme that virus detection item information and label information are embedded in a unique code of a reagent card, each type of reagent card is used for detecting a corresponding virus detection sample, such as a fluorescent reagent card is used for detecting an a sample, a B sample and a C sample, a colloidal gold reagent card is used for detecting a D sample, an E sample and an F sample, more specifically, the fluorescent reagent card with the unique code initial letter of "1" is used for detecting the a sample, the fluorescent reagent card with the initial letter of "2" is used for detecting the B sample, the fluorescent reagent card with the initial letter of "3" is used for detecting the C sample, the required virus test item information and label information for testing the a sample can be embedded in the unique code of the fluorescent reagent card with the initial letter of "1", the information of the required virus detection project, the label information and the like for detecting the B sample are embedded in the unique code of the fluorescent reagent card with the initial letter of 2. After the mobile terminal scans and identifies the virus detection item information contained in the unique code of the card board, the mobile terminal automatically acquires the virus detection item corresponding to the sample from the cloud and writes the virus detection item into the two-in-one fluorescent colloidal gold detection device, and acquires the label data corresponding to the virus detection item from the cloud and writes the label data into the two-in-one fluorescent colloidal gold detection device. Therefore, the automation of acquisition of the virus inspection project and the standard song data is realized, the proper project card and standard song card do not need to be selected manually, and the project card slot and the standard song card slot do not need to be reserved in the virus inspection equipment, so that the structure of the virus inspection equipment is simplified, the miniaturization design of the equipment is facilitated, and the production cost is reduced.
The invention also provides a drug detection device based on the fluorescent colloidal gold, which can realize the drug detection method, and as shown in fig. 9, the drug detection device comprises a mobile terminal 1 and fluorescent colloidal gold two-in-one detection equipment 2.
Specifically, as shown in fig. 10, the mobile terminal 1 includes:
the code scanning module is used for providing a device two-dimensional code for the fluorescent colloidal gold two-in-one detection device for a detection person to scan by calling a terminal camera, or the unique code of the fluorescent reagent card board, or the unique code of the colloidal gold reagent card board;
the communication connection module is connected with the code scanning module and used for establishing wireless communication connection with the fluorescent colloidal gold two-in-one equipment (the two-in-one equipment is provided with the communication module) after the two-dimensional code of the equipment is scanned;
the virus detection object personnel information input module is used for providing personnel for detection to input personnel information of the virus detection object;
the information binding module is connected with the code scanning module and the virus detection object personnel information input module and is used for carrying out information binding on the scanned card board coding information and the personnel information of the virus detection object and storing the binding information into a card board coding binding database;
the virus detection instruction generating and sending module is used for providing a virus detection instruction for detection personnel and sending the virus detection instruction to the fluorescent colloidal gold two-in-one equipment;
and after receiving the virus detection instruction, the fluorescent colloidal gold two-in-one detection equipment performs card reading comparison on the reagent card board to form a virus detection result related to a virus detection object and pushes the virus detection result to the mobile terminal.
In order to prevent false detection of poison caused by secondary card binding, as shown in fig. 9-10, the mobile terminal further includes:
a reagent card binding record inquiry module which is connected with the code scanning module and is used for inquiring whether the currently scanned unique code of the fluorescent reagent card or the colloidal gold reagent card has binding record or not from the card code binding database after the unique code of the fluorescent reagent card or the colloidal gold reagent card is scanned,
if yes, generating prompt information that the current card board cannot be bound and displaying the prompt information to the detection personnel;
if not, no response is made;
the virus inspection item cloud acquisition module is connected with the code scanning module and is used for automatically acquiring a virus inspection item to be detected from the cloud 3 after scanning and identifying required virus inspection item information contained in the unique code of the card;
the cloud acquisition module for the virus inspection label data is connected with the code scanning module and is used for automatically acquiring label data corresponding to a virus inspection item from the cloud 3 after the virus inspection label data information contained in the unique code of the card is scanned and identified;
and the data writing module is connected with the virus detection project cloud acquisition module and the virus detection standard curve data cloud acquisition module and is used for writing the acquired virus detection project and standard curve data into the fluorescent colloidal gold two-in-one detection device.
In order to further standardize the poison and examine the flow, further reduce the poison and examine the false probability, drug detection device is still including being used for escort the intelligent material evidence case 4 of fluorescence reagent cardboard and/or colloidal gold reagent cardboard, sweep the equipment two-dimensional code that a yard module still is used for sweeping intelligent material evidence case 4 in mobile terminal 1, communication connection module still is used for sweeping the wireless communication connection who realizes and intelligent material evidence case 4 (having communication module in the intelligent material evidence case) behind the equipment two-dimensional code, still include in mobile terminal 1:
the unpacking password setting module is used for setting and storing the unpacking passwords of the intelligent material evidence box for escort personnel;
the box locking command generating and sending module is connected with the box opening password setting module and used for automatically generating a box locking command and sending the box locking command to the intelligent material evidence box after the box opening password is set;
the box opening password input module is used for providing box opening personnel with a box opening password to input the box opening password;
an unpacking password verification module connected with the unpacking password input module and used for comparing the input unpacking password with the stored unpacking password,
if the comparison is successful, generating a box opening instruction and sending the box opening instruction to the intelligent material evidence box;
if the comparison fails, generating a password error prompt message to display the unpacking personnel;
as shown in fig. 11, the intelligent material evidence box 4 includes:
the instruction receiving module is used for receiving a box locking instruction or a box opening instruction sent by the mobile terminal;
the control module is connected with the instruction receiving module and is used for controlling the box locking mechanism to execute box locking actions according to the received box locking instructions so as to lock the intelligent material evidence box or controlling the box opening mechanism to execute box opening actions according to the received box opening instructions so as to open the intelligent material evidence box;
and the communication module is used for establishing wireless communication connection with the mobile terminal.
In conclusion, the virus detection process of the two-in-one detection device is controlled by the mobile terminal, the intelligent level of the two-in-one detection device is improved, and the virus detection control function of the two-in-one detection device is transferred to the mobile terminal, so that the internal structure of the device is simplified, the size of the device is reduced, and the convenience in use of the device is improved; the unique code is printed on the reagent card, and the personnel information of the virus detection object is bound with the unique code information of the card, so that the phenomena of false detection of false name and false detection and missed detection of wrong detection are effectively avoided, and the virus detection process is standardized; by verifying the equipment operation authority of the detection personnel, the condition that irrelevant personnel are in disorder detection is effectively prevented, and the virus detection process is further standardized; the reagent card boards are escorted through the intelligent material evidence box, the reagent card boards with specific quantity are escorted for each virus detection task, and the possibility of virus detection and counterfeiting is further reduced.
It should be understood that the above-described embodiments are merely preferred embodiments of the invention and the technical principles applied thereto. It will be understood by those skilled in the art that various modifications, equivalents, changes, and the like can be made to the present invention. However, such variations are within the scope of the invention as long as they do not depart from the spirit of the invention. In addition, certain terms used in the specification and claims of the present application are not limiting, but are used merely for convenience of description.

Claims (9)

1. A method for detecting drugs based on fluorescent colloidal gold is characterized by comprising the following steps:
step S1, starting the fluorescent colloidal gold two-in-one detection device;
step S2, the mobile terminal establishes wireless communication connection with the fluorescent colloidal gold two-in-one detection device in a scanning device two-dimensional code mode;
step S3, inputting the personnel information of the virus detection object;
step S4, scanning the unique code of the fluorescent reagent card or the colloidal gold reagent card through the mobile terminal;
step S5, the mobile terminal binds the scanned card board coding information and the personnel information of the virus detection object;
step S6, pushing the fluorescent reagent card board or the colloidal gold reagent card board loaded with the toxicity test sample into a device detection port;
step S7, a poison detection instruction is sent to the fluorescent colloidal gold two-in-one detection device through the mobile terminal;
step S8, after receiving the virus detection instruction, the two-in-one fluorescent colloidal gold detection equipment reads the card of the reagent card and compares the card to form a virus detection result related to the virus detection object and pushes the virus detection result to the mobile terminal;
and step S9, the mobile terminal forms the virus detection result into a virus detection record and stores the virus detection record.
2. The method for detecting narcotics based on fluorescent colloidal gold according to claim 1, wherein in step S4, after the unique code of the fluorescent reagent card or the colloidal gold reagent card is scanned, the mobile terminal queries whether the scanned unique code of the card has an over-binding record from a card code binding record library,
if so, prompting a detector that the current card board has a binding record and refusing to bind again;
if not, the process proceeds to step S5.
3. The method for detecting drugs based on fluorescent colloidal gold according to claim 1, wherein the fluorescent reagent card printed with a unique code or the colloidal gold reagent card is escorted to the drug detection site through a smart material evidence box, and the method for intelligently escorting the fluorescent reagent card or the colloidal gold reagent card comprises:
l1, scanning the two-dimensional codes of the intelligent material evidence box through the mobile terminal to establish mutual wireless communication connection;
step L2, after the fluorescent reagent card board and/or the colloidal gold reagent card board are/is placed in the intelligent material evidence box and a box cover is covered, a box opening password is set through the mobile terminal, and then a box locking instruction is sent to the intelligent material evidence box;
step L3, after the controller of the intelligent material evidence box receives the box locking instruction, controlling a box locking mechanism to execute a box locking action so as to lock the intelligent material evidence box;
step L4, after the intelligent material evidence box is escorted to a drug detection site, a detection person inputs the box opening password set in the step L2 through the mobile terminal and sends a box opening instruction to the intelligent material evidence box;
and L5, after receiving the unpacking command, the controller of the intelligent material evidence box controls an unpacking mechanism to execute unpacking actions so as to open the intelligent material evidence box.
4. The method for detecting the drug based on the fluorescent colloidal gold as claimed in claim 1, wherein the unique code printed on the fluorescent reagent card or the colloidal gold reagent card contains the required drug detection item information, and the mobile terminal automatically obtains the drug detection item corresponding to the sample from the cloud after scanning and identifying the drug detection item information contained in the card unique code and writes the drug detection item into the fluorescent colloidal gold two-in-one detection device.
5. The method for detecting the drugs based on the fluorescent colloidal gold according to claim 1 or 4, wherein the unique code printed on the fluorescent reagent card or the colloidal gold reagent card contains the required virus detection label information, and after the mobile terminal scans and identifies the virus detection label information contained in the card unique code, the mobile terminal automatically obtains the label data corresponding to the virus detection project from a cloud and writes the label data into the fluorescent colloidal gold two-in-one detection device.
6. The method for detecting narcotics based on fluorescent colloidal gold according to claim 1, wherein in step S2, after the device two-dimensional code of the fluorescent colloidal gold two-in-one detection device is scanned, the mobile terminal actively obtains the device code information of each device having operation authority of the current login account from the database and compares the device code information with the currently scanned two-in-one device code information,
if the comparison is successful, establishing wireless communication connection with the two-in-one detection device;
if the comparison fails, the wireless communication connection with the two-in-one detection device is refused to be established.
7. A drug detection device based on the fluorescent colloidal gold, which can implement the drug detection method according to any one of claims 1 to 6, wherein the drug detection device comprises a mobile terminal and a fluorescent colloidal gold two-in-one detection device, and the mobile terminal comprises:
the code scanning module is used for providing a detection person to call a terminal camera to scan the two-dimensional code of the fluorescent colloidal gold two-in-one detection device, or the unique code of the fluorescent reagent card board, or the unique code of the colloidal gold reagent card board;
the communication connection module is connected with the code scanning module and used for establishing wireless communication connection with the fluorescent colloidal gold two-in-one device after scanning the two-dimensional code of the device;
the virus detection object personnel information input module is used for providing the personnel for the detection to input personnel information of the virus detection object;
the information binding module is connected with the code scanning module and the personnel information input module of the virus detection object and is used for carrying out information binding on the scanned card board coding information and the personnel information of the virus detection object and storing the binding information into a card board coding binding database;
the virus detection instruction generating and sending module is used for providing the virus detection instruction for the detection personnel to generate and send the virus detection instruction to the fluorescent colloidal gold two-in-one equipment;
and after the fluorescent colloidal gold two-in-one detection equipment receives the virus detection instruction and then performs card reading comparison on the reagent card, forming a virus detection result related to the virus detection object and pushing the virus detection result to the mobile terminal.
8. The fluorescent colloidal gold-based drug detection device of claim 7, wherein the mobile terminal further comprises:
a reagent card binding record inquiry module which is connected with the code scanning module and is used for inquiring whether the currently scanned unique code of the fluorescent reagent card or the colloidal gold reagent card has binding record or not from a card code binding database after the unique code of the fluorescent reagent card or the colloidal gold reagent card is scanned,
if yes, generating prompt information that the current card board cannot be bound and displaying the prompt information to the detection personnel;
if not, no response is made;
the virus inspection item cloud acquisition module is connected with the code scanning module and is used for automatically acquiring a virus inspection item to be detected from the cloud after scanning and identifying required virus inspection item information contained in the unique code of the card;
the cloud acquisition module for the virus inspection label data is connected with the code scanning module and is used for automatically acquiring label data corresponding to the virus inspection item from the cloud after the virus inspection label data information contained in the unique code of the card is scanned and identified;
and the data writing module is connected with the virus detection project cloud acquisition module and the virus detection standard curve data cloud acquisition module and is used for writing the acquired virus detection project and standard curve data into the fluorescent colloidal gold two-in-one detection device.
9. The fluorescent colloidal gold-based drug detection device according to claim 7 or 8, further comprising an intelligent physical evidence box for escorting the fluorescent reagent card board and/or the colloidal gold reagent card board, wherein the code scanning module in the mobile terminal is further configured to scan a two-dimensional code of the intelligent physical evidence box, the communication connection module is further configured to achieve wireless communication connection with the intelligent physical evidence box after scanning the two-dimensional code of the equipment, and the mobile terminal further comprises:
the unpacking password setting module is used for providing the escort personnel with the unpacking passwords of the intelligent material evidence box and storing the unpacking passwords;
the box locking instruction generating and sending module is connected with the box opening password setting module and used for automatically generating a box locking instruction after the box opening password is set and sending the box locking instruction to the intelligent material evidence box;
the box opening password input module is used for providing the box opening person with the box opening password to input the box opening password;
an unpacking password verification module connected with the unpacking password input module and used for comparing the input unpacking password with the stored unpacking password,
if the comparison is successful, generating a box opening instruction and sending the box opening instruction to the intelligent material evidence box;
if the comparison fails, generating a password error prompt message to display the unpacking personnel;
include in the intelligence material evidence case:
the instruction receiving module is used for receiving the box locking instruction or the box opening instruction sent by the mobile terminal;
and the control module is connected with the instruction receiving module and used for receiving the locking box instruction to control the locking box mechanism to execute the locking box action so as to lock the intelligent material evidence box or receiving the unlocking instruction to control the unlocking mechanism to execute the unlocking action so as to open the intelligent material evidence box.
CN202111215888.7A 2021-10-19 2021-10-19 Drug detection method and device based on fluorescent colloidal gold Pending CN113945550A (en)

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