CN113916732A - Active sludge microscopic image real-time observation recording pulse flow cell - Google Patents
Active sludge microscopic image real-time observation recording pulse flow cell Download PDFInfo
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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Abstract
The invention discloses an activated sludge microscopic image real-time observation recording pulse flow cell, which comprises a sample introduction reflux system, a flow cell (2) and a microscopic image acquisition analysis system, wherein the flow cell (2) is provided with a cavity, and the sample introduction reflux system is used for injecting a sludge suspension into the cavity of the flow cell (2); the microscopic image acquisition and analysis system comprises a microscope (31) and a processor (32), wherein a microscopic picture of sludge suspension in the flow cell (2) is acquired through the microscope (31), and the microscopic picture is analyzed and recorded through the processor (32). The activated sludge micro-image real-time observation recording pulse flow cell provided by the invention solves the problem that the sludge micro morphological parameters such as the typing characteristics of the activated sludge are difficult to extract on line, and realizes automatic sampling and real-time observation and recording of the micro morphology and the dynamic characteristics of the activated sludge flocs.
Description
Technical Field
The invention relates to an activated sludge microscopic image real-time observation recording pulse flow cell system, and belongs to the technical field of sewage treatment.
Background
In the field of water treatment, the activated sludge process is an effective method for treating sewage, and sludge-water separation in the sedimentation process is an important treatment process. Researches show that the deterioration of the settleability of the activated sludge causes the problems of easy sludge loss, increased effluent suspended matters, reduced sewage treatment capacity and the like, and once the deterioration of the settleability of the activated sludge occurs, a long time is required for the activated sludge to recover, so that the real-time understanding of the quality of the activated sludge and the adoption of corresponding measures are very important.
In practical application, the activated sludge settleability is generally characterized by a Sludge Volume Index (SVI), but earlier researches prove that the macroscopic settleability of the activated sludge is determined by the comprehensive action of some microscopic factors such as the surface property and morphological structure of sludge flocs, the size distribution characteristics of the flocs, filamentous fungi and the like.
The microscopic factors can be detected through a microscope, and are all detected manually at present, but the detection result of manual detection is influenced by subjective factors of an observer, and the defects of low efficiency, large delay and the like exist in manual detection.
Therefore, there is a need to develop a system that can acquire microscopic images of activated sludge quickly, stably, in real time, and with low latency.
Disclosure of Invention
In order to overcome the above problems, the present inventors have conducted intensive studies to provide the following:
on one hand, the invention provides an activated sludge micro-image real-time observation recording pulse flow cell, which comprises a sample introduction reflux system, a flow cell 2 and a micro-image acquisition analysis system,
the flow cell 2 is provided with a cavity, and the sample introduction reflux system is used for injecting sludge suspension into the cavity of the flow cell 2.
The microscopic image acquisition and analysis system comprises a microscope 31 and a processor 32, wherein a microscopic picture of sludge suspension in the flow cell 2 is acquired through the microscope 31, and the microscopic picture is analyzed and recorded through the processor 32.
Further, the sample return system comprises a first sample inlet pipe 11, a sample tank 12, a return tank 13, a first return pipe 14 and a second sample inlet pipe 15,
the first sample inlet pipe 11 continuously takes out the active sludge suspension from the reactor or the biochemical pool and conveys the active sludge suspension to the sample inlet tank 12, the sample inlet tank 12 is positioned above or inside the reflux tank 13, so that after the sample inlet tank 12 is filled with the sludge suspension, the sludge suspension overflows into the reflux tank 13, and the sludge suspension in the reflux tank 13 flows back to the reactor or the biochemical pool through the first reflux pipe 14;
one end of the second sample inlet pipe 15 is connected with the flow cell 2, and the other end is positioned in the sample inlet tank 12, so that sludge suspension in the sample inlet tank 12 is transferred to the cavity of the flow cell 2.
Further, a first sample injection pump 111 is disposed on the first sample injection pipe 11, a first reflux pump 141 is disposed on the first reflux pipe 14, a second sample injection pump 151 is disposed on the second sample injection pipe 15, the sample injection speed of the first sample injection pipe 11, the reflux speed of the first reflux pipe and the sample injection speed of the second sample injection pipe 15 are respectively controlled,
preferably, the sample injection speed of the first sample injection pipe 11 is 10 times higher than that of the second sample injection pipe 15.
Preferably, the first reflux pump 141 with a proper discharge capacity is selected to ensure that the time for transferring the sludge suspension from the reactor or the biochemical pool to the sample tank 12 is not more than 1s, and the second sample pump 151 with a proper discharge capacity is selected to ensure that the time for transferring the sludge suspension from the sample tank 12 to the flow cell 2 is not more than 10 s.
Preferably, the discharge capacity of the second sample pump 151 is adjustable, and at the initial time, the second sample pump 151 samples with a large discharge capacity, so that the flow cell is filled with the activated sludge suspension rapidly, and at the subsequent time, the second sample pump 151 samples with a small discharge capacity, so that the sludge suspension in the flow cell flows slowly.
Preferably, the sample return system further comprises a pressure pipe 16, one end of the pressure pipe 16 is connected to the flow cell 2, the other end is located in the sample tank 12, and a pressure pump 161 is disposed on the pressure pipe 16.
Preferably, the sample introduction reflux system further comprises a water tank 17, the water tank 17 is connected with the flow cell 2 through a dilution water pipe 18, and distilled water is placed in the water tank 17 and used for diluting the activated sludge suspension.
Preferably, the activated sludge micro-image real-time observation recording pulse flow cell further comprises a cleaning system, the cleaning system comprises a cleaning pipe 41, a cleaning valve 42 is arranged on the cleaning pipe 41, and the cleaning pipe 41 is connected with an inlet of the cavity of the flow cell 2.
On the other hand, the invention also discloses a real-time observation and recording method of the activated sludge microscopic image, which is preferably realized by adopting the activated sludge microscopic image real-time observation and recording pulse flow cell, and comprises the following steps:
s1, communicating the sample feeding groove with the reactor or the biochemical pool to enable the sludge suspension in the sample feeding groove to be synchronous with the sludge suspension in the reactor or the biochemical pool;
s2, transferring the sludge suspension in the sample feeding groove to a flow cell for microscopic observation and recording.
Preferably, in step S1, the sample feeding tank is communicated with the reactor or the biochemical tank through the first sample feeding pipe, the reflux tank and the first reflux pipe, and the time for transferring the sludge suspension from the reactor or the biochemical tank to the sample feeding tank 12 is not more than 1S.
The invention has the advantages that:
(1) the problem that the micro morphological parameters of the sludge such as the typing characteristics of the active flocs and the like are difficult to extract on line is solved;
(2) the automatic sampling and the real-time observation and recording of the microscopic morphology and the dynamic characteristics of the activated sludge flocs are realized;
(3) the long-term stable observation and recording can be realized, and the observation and analysis result is accurate.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of an activated sludge micro-image real-time observation recording pulse flow-through cell according to a preferred embodiment of the invention;
FIG. 2 is a schematic diagram showing the structure of the bottom of a flow cell in an activated sludge micro-image real-time observation recording pulse flow cell according to a preferred embodiment of the invention;
FIG. 3 is a schematic diagram illustrating an upper cover structure of a flow cell in a pulse flow cell for observing and recording a microscopic image of activated sludge in real time according to a preferred embodiment of the invention;
FIG. 4 is a schematic diagram showing a channel controller structure in an activated sludge micro-image real-time observation recording pulse flow-through cell according to a preferred embodiment of the invention;
FIG. 5 is a schematic diagram illustrating an anti-edge strip structure in an activated sludge micro-image real-time observation recording pulse flow cell according to a preferred embodiment of the invention;
FIG. 6 is a schematic view showing a sectional state of a flow cell when a channel controller in a recording pulse flow cell is pressed down for real-time observation of a microscopic image of activated sludge according to a preferred embodiment of the present invention;
FIG. 7 is a schematic diagram showing a sectional state of a flow cell after an activated sludge micro-image is observed and recorded in real time and a channel controller in a pulse flow cell is moved upwards according to a preferred embodiment of the invention;
FIG. 8 is a partial picture taken by a microscope in example 1;
FIG. 9 shows the two-dimensional fractal dimension results for sludge flocs in the picture of example 1;
FIG. 10 shows the one-dimensional entropy results of the sludge flocs in the picture of example 1.
The reference numbers illustrate:
2-a flow-through cell;
11-a first sample introduction tube;
12-a sample feeding groove;
13-a reflux tank;
14-a first return pipe;
15-a second sample injection tube;
16-a pressurized tube;
17-a water tank;
18-a dilution water pipe;
19-a second return conduit;
21-pool bottom;
22-putting a pool cover;
23-a path controller;
31-a microscope;
32-a processor;
41-cleaning tube;
42-a purge valve;
111-a first sample pump;
141-a first reflux pump;
151-a second sample injection pump;
161-pressure pump;
181-a dilution pump;
211-grooves;
212-support column;
213-a support plate;
214-anti-falling edge strips;
231-a viewing aperture;
312-microscope light source.
Detailed Description
The invention is explained in more detail below with reference to the figures and examples. The features and advantages of the present invention will become more apparent from the description.
The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments. While the various aspects of the embodiments are presented in drawings, the drawings are not necessarily drawn to scale unless specifically indicated.
On the one hand, the invention provides an activated sludge micro-image real-time observation recording pulse flow cell, which comprises a sample introduction reflux system, a flow cell 2 and a micro-image acquisition and analysis system.
The flow cell 2 is provided with a cavity, and the sample introduction reflux system is used for injecting the sludge suspension into the cavity of the flow cell 2;
2 cavity walls of flowcell are transparent material, the microscopic image acquisition analytic system includes microscope 31 and treater 32, gathers the microscopic picture of mud turbid liquid in the flowcell 2 through microscope 31, carries out analysis record to the microscopic picture through treater 32, obtains mud floc microscopic morphology and dynamic characteristics.
Furthermore, the sample introduction reflux system takes out the activated sludge suspension from the reactor or the biochemical pool, injects the activated sludge suspension into the cavity of the flow cell 2, and analyzes the flocs in the activated sludge through the microscopic image acquisition and analysis system.
The invention carries out the following technical design in order to ensure that the activated sludge suspension is injected into the flow cell with the lowest delay.
In the present invention, the sample return system includes a first sample inlet tube 11, a sample tank 12, a return tank 13, a first return tube 14, and a second sample inlet tube 15, as shown in fig. 1.
The first sample inlet pipe 11 continuously takes out the active sludge suspension from the reactor or the biochemical pool and conveys the active sludge suspension to the sample inlet tank 12, the sample inlet tank 12 is positioned above or inside the reflux tank 13, so that after the sample inlet tank 12 is filled with the sludge suspension, the sludge suspension overflows to the reflux tank 13, and the sludge suspension in the reflux tank 13 flows back to the reactor or the biochemical pool through the first reflux pipe 14.
In a preferred embodiment, the first sample inlet pipe 11 is connected to the bottom of the sample inlet tank 12, so that the activated sludge suspension moves from bottom to top in the sample inlet tank 12, and all the activated sludge suspension in the sample inlet tank 12 is in a moving state, thereby avoiding sludge deposition in the sample inlet tank 12.
In a preferred embodiment, the first return pipe 14 is located at the bottom end of the return tank 13, and the bottom of the return tank 13 is tapered or flared to facilitate the sludge suspension therein to enter the first return pipe.
One end of the second sample inlet pipe 15 is connected with the flow cell 2, and the other end is positioned in the sample inlet tank 12, so that sludge suspension in the sample inlet tank 12 is transferred to the cavity of the flow cell 2.
Further, the length of the second sample introduction pipe 15 is less than 0.25 meter.
In the invention, a circulating pipeline is established between the sample introduction tank 12 and the reactor or the biochemical tank through the first sample introduction pipe 11, the sample introduction tank 12, the reflux tank 13 and the first reflux pipe 14, so that sludge suspension in the sample introduction tank 12 and sludge suspension in the reactor or the biochemical tank are synchronous, and the activity of the sludge suspension in the sample introduction tank 12 is maintained. Further, the length of the second sampling pipe 15 is greatly reduced by the circulation, so that the second sampling pipe 15 can quickly inject the activated sludge suspension into the flow cell 2, and the activity of the sludge suspension subjected to microscopic analysis is ensured.
According to the present invention, the first sample inlet pipe 11 is provided with a first sample pump 111, the first reflux pipe 14 is provided with a first reflux pump 141, and the second sample inlet pipe 15 is provided with a second sample pump 151, which respectively control the sample inlet speed of the first sample inlet pipe 11, the reflux speed of the first reflux pipe, and the sample inlet speed of the second sample inlet pipe 15.
In a preferred embodiment, the sample introduction speed of the first sample introduction pipe 11 is 10 times or more, preferably 100 times or more higher than that of the second sample introduction pipe 15, and synchronization between the sludge suspension in the sample introduction tank 12 and the sludge suspension in the reactor or biochemical tank is ensured by high-speed sample introduction of the first sample introduction pipe 11.
In a more preferred embodiment, the displacement of the first reflux pump 141 is greater than that of the first reflux pump 141, so as to ensure that the sludge suspension in the reflux tank 13 can be rapidly transported back to the reactor or the biochemical pool, and avoid activity reduction caused by precipitation and the like of the sludge suspension in the reflux tank 13.
In a preferred embodiment, the sample injection speed of the second sample injection tube 15 is less than 1mL/min, the sample injection speed of the first sample injection tube 11 is not less than 300mL/min, preferably 500mL/min, and the reflux speed of the first reflux tube 14 is not less than 400mL/min, preferably 600 mL/min.
In a preferred embodiment, the first reflux pump 141 with a proper discharge capacity is selected, so that the time for transferring the sludge suspension from the reactor or the biochemical tank to the sample tank 12 is not more than 1s, and the second sample pump 151 with a proper discharge capacity is selected, so that the time for transferring the sludge suspension from the sample tank 12 to the flow cell 2 is not more than 10s, thereby not only ensuring the activity of the sludge suspension, but also reducing the analysis delay, and further realizing the effect of observing and recording the microscopic morphology and dynamic characteristics of the active sludge flocs in real time and with low delay.
In a preferred embodiment, the flow rate of the second sample pump 151 is adjustable, at the initial time, the second sample pump 151 samples at a large flow rate, so that the flow cell is quickly filled with the activated sludge suspension, and at the subsequent time, the second sample pump 151 samples at a small flow rate, so that the sludge suspension in the flow cell slowly flows, and thus, the continuous observation and analysis are facilitated.
According to a preferred embodiment of the present invention, the second sample injection pump 151 injects a sample at a large flow rate, which means that the sample injection speed of the second sample injection pipe 15 is greater than 15mL/min, and the small flow rate refers to that the sample injection speed of the second sample injection pipe 15 is less than 1 mL/min.
More preferably, in order to maintain the passing performance of the flow cell, the sample injection speed of the second sample injection tube 15 is periodically adjusted, and the passage of the flow cell is enlarged in cooperation with the flow cell passage control device, so that the flow cell is purged in a pulse manner.
For example, 60s is used as one cycle, the large flow time is 10s, and the small flow time is 50 s.
The inventor finds that the microscopic image acquisition and analysis system requires the sludge suspension to be in a low-flow-rate state when detecting the sludge suspension in the flow cell 2, and a small amount of activated sludge flocs can be adhered to the inner wall of the flow cell 2 after 5-10 min, so that the observation and analysis accuracy is influenced.
In the present invention, the sample introduction reflux system further comprises a pressure pipe 16, one end of the pressure pipe 16 is connected to the flow cell 2, the other end is located in the sample introduction tank 12, and a pressure pump 161 is disposed on the pressure pipe 16, as shown in fig. 1.
Further, at regular intervals, the pressurizing pump 161 is turned on, and large-flow sludge suspension is pumped into the flow cell 2 through the pressurizing pipe 16, so that activated sludge flocs adhered to the inner wall of the flow cell are washed away, and the trafficability of the flow cell 2 is maintained.
Preferably, the flow rate of the pressurizing pipe 16 is not less than 15 mL/min.
The inventors have found that when the concentration of the activated sludge suspension is too high, for example, when MLSS is greater than 5000mg/L, the observation and analysis effect is not good, and it is necessary to dilute the activated sludge suspension, and according to a preferred embodiment of the present invention, the activated sludge suspension is proportionally diluted to 5000mg/L or less according to the concentration of the activated sludge suspension in the actual feed water before the collection of the microscopic image.
In a preferred embodiment, the sample return system further comprises a water tank 17, the water tank 17 is connected to the flow cell 2 through a dilution water pipe 18, and distilled water is placed in the water tank 17 for diluting the activated sludge suspension.
Further, a dilution pump 181 is provided in the dilution water pipe 18 to control the flow rate of the dilution water.
Further, according to the present invention, the sample return system further comprises a second return pipe 19 for conveying the sludge suspension flowing out of the flow cell 2 back to the reactor or the biochemical cell.
In a preferred embodiment, the first sample injection pump, the first reflux pump, the second sample injection pump, the pressurizing pump and the diluting pump are peristaltic pumps, and the peristaltic pumps do not damage the form of the activated sludge and are easy to control the flow rate.
In a preferred embodiment, the chamber wall of the flow cell 2 is made of a transparent material with a visible light transmittance of more than 90%, and has a certain hardness and mechanical strength to prolong the service life, such as quartz, glass, transparent plastic, gel organic glass, and the like.
Preferably, the flow cell 2 is a cube.
Preferably, the thickness of the cavity is 0.1-0.2 mm, so that a microscope can be well focused on sludge flocs in the cavity.
Preferably, the length of the chamber is no greater than 50mm to facilitate cleaning.
In a preferred embodiment, the flow cell 2 includes a cell bottom 21, an upper cell cover 22, and a pathway controller 23, as shown in FIGS. 2-5.
The pool bottom 21 is provided with a groove 211, the corner position of the groove 211 is provided with a support column 212, the upper pool cover 22 is arranged on the support column 212 and is attached to the pool bottom 21, so that a cavity is formed at the position of the groove 211.
Further, both ends of the chamber have openings to allow inflow and outflow of the sludge suspension.
Preferably, the two end openings are connected with the pipeline through rubber sleeves to realize sealing.
Furthermore, the upper tank cover 22 is a transparent flat plate with certain elasticity, such as an acrylic flat plate, the channel controller 23 is arranged at the upper end of the upper tank cover 22, the channel controller 23 is a mechanism capable of moving up and down, and the upper tank cover 22 is pressed down through the channel controller 23 to realize the control of the thickness of the cavity.
When the large-flow pulse washing is performed, the channel controller 23 moves upwards without pressing the upper tank cover 22, so that the upper tank cover 22 is restored to be in a flat plate shape, and the volume of the cavity is larger, so that large granular sludge can normally pass through the cavity and is cleared through the whole flow-through tank, as shown in fig. 6;
when low-flow-rate observation is required, the passage controller 23 moves down to press the upper tank cover 22, so that the upper tank cover 22 deforms downward, the thickness of the cavity is narrowed, and the sludge is compressed to the same focal plane, so that the sludge can be conveniently shot by a microscope, as shown in fig. 7.
Preferably, when a low flow rate observation is required, after the passage controller 23 is moved down, the distance between the upper tank cover 22 and the bottom surface of the groove 211 is not more than 0.2mm, i.e. the thickness of the cavity is not more than 0.2 mm.
In a preferred embodiment, the passage controller 23 is provided at a central position of the upper tank cover 22, so as to control the deformation amount of the upper tank cover 22.
Furthermore, the channel controller 23 is provided with an observing hole 231 penetrating up and down, so that the microscope lens can observe the inside of the cavity of the flow cell 2 through the channel controller 23.
In a preferred embodiment, the lower end of the path controller 23 is curved to reduce stress damage to the upper tank cover 22 when the path controller presses down the upper tank cover 22.
Further, in the present invention, the specific mechanism for moving the path controller 23 up and down is not particularly limited, and those skilled in the art can freely set the path controller according to actual needs, for example, the path controller is implemented by a screw motor.
In a preferred embodiment, a supporting plate 213 is further disposed inside the groove 211, preferably at the edge of the groove 211, the supporting plate 213 protrudes from the bottom of the groove 211, preferably by a height not greater than 0.2mm, so that after the upper tank cover 22 is deformed by pressure, a gap is formed between the upper tank cover 22 and the bottom of the groove 211, thereby avoiding the blockage of the flow cell 2 caused by complete compression.
In a more preferable real-time protection, an anti-separation edge strip 214 is further disposed at the edge of the pool bottom 21, and the anti-separation edge strip 214 is disposed at the top end of the upper pool cover 22 to prevent the upper pool cover 22 from being separated from the groove 211 after moving on the channel controller 23.
According to a preferred embodiment of the present invention, the inlet and the outlet of the cavity of the flow cell 2 are tubular, and the second sample inlet tube 15, the pressurizing tube 16 and the dilution water tube 18 are merged into one tube and then connected with the inlet of the cavity, preferably through a rubber tube.
Further, the flow cell 2 is detachably fixed to the stage of the microscope 31, and the flow cell 2 can be cleaned or replaced when it is dirty.
According to the present invention, the microscope 31 has an electron eyepiece thereon to capture a microscope image for transmission to the processor 32, and the stage is located between the objective of the microscope 31 and the microscope light source 312.
In the present invention, the type of the microscope 31 is not particularly limited, and may be a digital microscope, a stereoscopic microscope, a general optical microscope, an inverted optical microscope, a fluorescence microscope, a multi-light source microscope, a confocal laser microscope, or the like, and preferably, the microscope 31 has a high-speed camera to improve the quality of the captured image.
In the invention, the processor 32 identifies sludge flocs in the image, analyzes fractal dimension and information entropy parameters, and expresses the state of the activated sludge through the fractal dimension and the information quotient parameters.
The inventor finds that the activity state of the sludge has a corresponding relation with the fractal dimension and the information quotient of the sludge flocs under the microscopic image, and the lower the sludge activity is, the lower the fractal dimension of the sludge flocs in the microscopic image is, and the higher the information quotient of the sludge flocs is.
Further preferably, in the invention, the sludge state in the reactor or biochemical pool is monitored by recording the two-dimensional fractal dimension and the one-dimensional information entropy of the sludge flocs in the microscopic image.
Fractal dimension and one-dimensional information entropy are common concepts in image processing, and detailed description is not provided in the specific calculation process of the invention.
In a preferred embodiment, the activated sludge micro-image real-time observation recording pulse flow cell further comprises a cleaning system, the cleaning system comprises a cleaning pipe 41, a cleaning valve 42 is arranged on the cleaning pipe 41, the cleaning pipe 41 is connected with an inlet of the cavity of the flow cell 2, and when the sludge micro-observation is not performed, a cleaning solution is introduced into the flow cell 2 through the cleaning pipe 41 to clean the flow cell 2, so that the light transmittance of the flow cell is ensured.
Preferably, the washing liquid is a hydrogen peroxide solution.
On the other hand, the invention also provides a real-time observation and recording method of the activated sludge microscopic image, which is realized by adopting the activated sludge microscopic image real-time observation and recording pulse flow cell and comprises the following steps:
s1, communicating the sample feeding groove with the reactor or the biochemical pool to enable the sludge suspension in the sample feeding groove to be synchronous with the sludge suspension in the reactor or the biochemical pool;
s2, transferring the sludge suspension in the sample feeding tank to a flow cell for microscopic observation and recording;
preferably, the method further comprises a step S3 of cleaning the circulation tank.
In step S1, the sample inlet tank is communicated with the reactor or the biochemical pool through the first sample inlet tube, the reflux tank and the first reflux tube.
The first sample inlet pipe continuously takes out the active sludge suspension from the reactor or the biochemical pool and conveys the active sludge suspension to the sample inlet tank, after the sample inlet tank is filled with the sludge suspension, the sludge suspension overflows to the reflux tank, and the sludge suspension in the reflux tank flows back to the reactor or the biochemical pool through the first reflux pipe.
Preferably, the sample injection speed of the first sample injection pipe 11 is not less than 300mL/min, preferably 500mL/min, and the reflux speed of the first reflux pipe 14 is not less than 400mL/min, preferably 600 mL/min.
Preferably, the time for transferring the sludge suspension from the reactor or the biochemical pond to the sample feeding groove 12 is not more than 1 s.
In step S2, the sludge suspension in the sample tank is transferred to the flow cell through the second sample inlet tube.
Preferably, the sample injection speed of the second sample injection pipe is less than 1 ml/min.
Preferably, the sample injection speed of the first sample injection pipe is 10 times higher than that of the second sample injection pipe, and preferably 100 times higher.
Preferably, the time for transferring the sludge suspension from the sample feeding groove to the flow cell is not more than 10 s.
Preferably, the sampling speed of the second sampling tube is adjustable, at an initial time, the sampling speed of the second sampling tube is fast, for example, greater than 15mL/min, so that the flow cell is quickly filled with the activated sludge suspension, and at a subsequent time, the sampling speed of the second sampling tube is slow, for example, less than 1mL/min, so that the sludge suspension in the flow cell slowly flows, and the continuous observation and analysis are facilitated.
Preferably, the sampling speed of the second sampling tube is adjusted once every certain time, for example, every 1 minute, and the rapid and slow sampling is repeated, so that the observed and recorded microscopic image has periodicity.
Preferably, at intervals, pumping a large-flow sludge suspension into the flow cell through the pressurizing pipe to flush away activated sludge flocs adhered to the inner wall of the flow cell, wherein the large flow refers to the flow not less than 15 mL/min.
Preferably, when the MLSS of the activated sludge suspension is more than 5000mg/L, the sludge suspension entering the flow cell is diluted through a dilution water pipe.
In step S2, a microscopic image of the sludge suspension in the flow cell is taken by a microscope, and sludge flocs in the recorded image are identified and analyzed by the processor.
In step S3, a washing solution is introduced into the flow cell through the washing tube to wash the flow cell, and preferably, the washing solution is a hydrogen peroxide solution.
Examples
Example 1
A simulation experiment is carried out by adopting the device shown in figure 1, and the device is verified to analyze and record the performance of the sludge suspension.
The sample introduction speed of the first sample introduction pipe 11 is 500mL/min, the reflux speed of the first reflux pipe 14 is 600mL/min, the second sample introduction pump 151 performs pulse type circulation sample introduction with 60s as a period, wherein the sample introduction is performed at a flow rate of 20mL/min for 10s, the sample introduction is performed at a flow rate of 0.5mL/min for 50s, the height of the support plate 213 of the flow cell 2 protruding out of the bottom of the groove 211 is 0.2mm, and the length of the cavity is 40 mm.
And (3) treating a sludge suspension sample to poison the sludge, and detecting the poisoning process of the sludge.
In the detection process, a part of pictures obtained by taking pictures through a microscope is shown in fig. 8, wherein the lower part of the pictures indicates the shooting time, and the sludge activity is normal at 0 minute.
The two-dimensional fractal dimension result corresponding to the sludge flocs in the picture is shown in fig. 9;
the one-dimensional information entropy results corresponding to the sludge flocs in the picture are shown in fig. 10.
As can be seen from figures 8-10, the device can be fine and continuously detect and analyze the sludge suspension, the problem that the micro form of the sludge is difficult to extract on line is solved, long-term stable observation and recording can be realized, and the observation and analysis result is accurate.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", and the like indicate orientations or positional relationships based on operational states of the present invention, and are only used for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise specifically stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; the connection may be direct or indirect via an intermediate medium, and may be a communication between the two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The present invention has been described above in connection with preferred embodiments, but these embodiments are merely exemplary and merely illustrative. On the basis of the above, the invention can be subjected to various substitutions and modifications, and the substitutions and the modifications are all within the protection scope of the invention.
Claims (10)
1. An activated sludge micro-image real-time observation recording pulse flow cell is characterized by comprising a sample introduction reflux system, a flow cell (2) and a micro-image acquisition analysis system,
the flow cell (2) is provided with a cavity, and the sample introduction reflux system is used for injecting sludge suspension into the cavity of the flow cell (2);
the microscopic image acquisition and analysis system comprises a microscope (31) and a processor (32), wherein a microscopic picture of sludge suspension in the flow cell (2) is acquired through the microscope (31), and the microscopic picture is analyzed and recorded through the processor (32).
2. The activated sludge microscopic image real-time observation recording pulse flow-through cell according to claim 1, characterized in that,
the sample introduction reflux system comprises a first sample introduction pipe (11), a sample introduction groove (12), a reflux groove (13), a first reflux pipe (14) and a second sample introduction pipe (15),
the first sample inlet pipe (11) continuously takes out the active sludge suspension from the reactor or the biochemical pool and conveys the active sludge suspension to the sample inlet tank (12), the sample inlet tank (12) is positioned above or inside the reflux tank (13), so that after the sample inlet tank (12) is filled with the sludge suspension, the sludge suspension overflows into the reflux tank (13), and the sludge suspension in the reflux tank (13) returns to the reactor or the biochemical pool through the first reflux pipe (14);
one end of the second sample inlet pipe (15) is connected with the flow cell (2), the other end of the second sample inlet pipe is positioned in the sample inlet groove (12), and sludge suspension in the sample inlet groove (12) is transferred into the cavity of the flow cell (2).
3. The activated sludge microscopic image real-time observation recording pulse flow-through cell according to claim 2, characterized in that,
a first sample feeding pump (111) is arranged on the first sample feeding pipe (11), a first reflux pump (141) is arranged on the first reflux pipe (14), a second sample feeding pump (151) is arranged on the second sample feeding pipe (15), the sample feeding speed of the first sample feeding pipe (11), the reflux speed of the first reflux pipe and the sample feeding speed of the second sample feeding pipe (15) are respectively controlled,
the sample introduction speed of the first sample introduction pipe (11) is higher than that of the second sample introduction pipe (15) by more than 10 times.
4. The activated sludge microscopic image real-time observation recording pulse flow-through cell according to claim 2, characterized in that,
the first backflow pump (141) with proper discharge capacity is selected, so that the time for transferring the sludge suspension from the reactor or the biochemical pool to the sample feeding tank (12) is not more than 1s, and the second backflow pump (151) with proper discharge capacity is selected, so that the time for transferring the sludge suspension from the sample feeding tank (12) to the flow cell (2) is not more than 10 s.
5. The activated sludge microscopic image real-time observation recording pulse flow-through cell according to claim 2, characterized in that,
the discharge capacity of the second sample injection pump (151) is adjustable, at the initial moment, the second sample injection pump (151) injects the sample with large discharge capacity, so that the flow cell is filled with the activated sludge suspension rapidly, and at the subsequent time, the second sample injection pump (151) injects the sample with small discharge capacity, so that the sludge suspension in the flow cell flows slowly.
6. The activated sludge microscopic image real-time observation recording pulse flow-through cell according to claim 2, characterized in that,
the sample injection reflux system further comprises a pressure pipe (16), one end of the pressure pipe (16) is connected with the flow cell (2), the other end of the pressure pipe is positioned in the sample injection groove (12), and a pressure pump (161) is arranged on the pressure pipe (16).
7. The activated sludge microscopic image real-time observation recording pulse flow-through cell according to claim 2, characterized in that,
the flow cell (2) comprises a cell bottom (21), an upper cell cover (22) and a channel controller (23), wherein the cell bottom (21) is provided with a groove (211), a supporting column (212) is arranged at the corner position of the groove (211), the upper cell cover (22) is arranged on the supporting column (212) and is attached to the cell bottom (21), so that a cavity is formed at the position of the groove (211);
go up pond lid (22) and be transparent elastic plate, access controller (23) are the mechanism that can reciprocate, set up in last pond lid (22) upper end, through pushing down pond lid (22), realize the control of cavity thickness.
8. The activated sludge microscopic image real-time observation recording pulse flow-through cell according to claim 2, characterized in that,
the activated sludge microimage real-time observation recording pulse flow cell further comprises a cleaning system, the cleaning system comprises a cleaning pipe (41), a cleaning valve (42) is arranged on the cleaning pipe (41), and the cleaning pipe (41) is connected with an inlet of a cavity of the flow cell (2).
9. A real-time observing and recording method of activated sludge microscopic images, which is preferably realized by adopting the activated sludge microscopic image real-time observing and recording pulse flow cell as claimed in any one of claims 1 to 8,
the method comprises the following steps:
s1, communicating the sample feeding groove with the reactor or the biochemical pool to enable the sludge suspension in the sample feeding groove to be synchronous with the sludge suspension in the reactor or the biochemical pool;
s2, transferring the sludge suspension in the sample feeding groove to a flow cell for microscopic observation and recording.
10. The method for observing and recording the microscopic image of the activated sludge in real time according to claim 9,
in step S1, the sample inlet tank is communicated with the reactor or the biochemical tank through the first sample inlet pipe, the reflux tank and the first reflux pipe, and the time for transferring the sludge suspension from the reactor or the biochemical tank to the sample inlet tank 12 is not more than 1S.
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