CN107271440A - The portable detector and method of a kind of parasite egg - Google Patents

The portable detector and method of a kind of parasite egg Download PDF

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Publication number
CN107271440A
CN107271440A CN201710529135.0A CN201710529135A CN107271440A CN 107271440 A CN107271440 A CN 107271440A CN 201710529135 A CN201710529135 A CN 201710529135A CN 107271440 A CN107271440 A CN 107271440A
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China
Prior art keywords
chamber
analytic unit
tiny sampler
observation
portable detector
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Pending
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CN201710529135.0A
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Chinese (zh)
Inventor
吕志跃
邬燕琪
徐月
徐一月
吴忠道
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Sun Yat Sen University
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Sun Yat Sen University
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Priority to CN201710529135.0A priority Critical patent/CN107271440A/en
Publication of CN107271440A publication Critical patent/CN107271440A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a kind of portable detector of parasite egg and method.Including fluid box, analytic unit and bearing;Injection port is provided with the top of the fluid box, middle part is provided with observation chamber, and bottom is provided with waste liquid chamber, and injection port, observation chamber and waste liquid chamber are sequentially connected logical by sample introduction runner;Tiny sampler filter assemblies are provided with the observation chamber;Peep hole is provided with position on the fluid box box body just to observing chamber;The analytic unit is gathering with camera and/or the analytic unit for handling image;Optical amplifier component is provided between the camera and peep hole.Device of the present invention can remove the complex operations such as artificial smear, micro- sem observation from, realize that the type of the quickly and easily tiny sampler such as worm's ovum judges and detected;It is not only simple in structure, it is with low cost, loss and false drop rate can be also substantially reduced, and the workload of operator is reduced, applicability is extensive, with larger application prospect.

Description

The portable detector and method of a kind of parasite egg
Technical field
The invention belongs to Bioexperiment equipment technical field.More particularly, to a kind of tiny sampler(Such as parasite worm Ovum)Portable detector and method.
Background technology
At present, the detection method for the parasite egg in fecal sample or urine mainly have direct smear inspection technique, Saturated salin e flotation method, water washing and precipitating method, polyamide fibre sieve pocket Fulleborn's method etc..
These above-mentioned methods are finally required for treated sample being applied on thin slice, pass through the side of manual observation Formula, observes detection in microscope.Laboratory technician needs to carry out smear by hand, using micro- sem observation, and according to the experience of itself The quantity for judging parasite egg is counted with knowledge, due to the subjectivity and fatiguability of people, is easily caused and is examined in detection process Survey efficiency is low, accuracy rate is low and labor intensity is big.
Above mentioned problem is summarized, is badly in need of simply and easily worm's ovum detection means, quickly, efficiently and accurately detects worm's ovum.
The content of the invention
The technical problems to be solved by the invention are to overcome lacking for parasite egg detection technique in existing excrement or urine Fall into and not enough there is provided a kind of portable detector of parasite egg, can remove artificial smear from using the present apparatus and operate, quickly Easily and efficiently realize the detection of worm's ovum in urine.The device could be applicable to the detection of other various tiny samplers, including each Plant micro-organisms, biological tissue and particulate material.
It is an object of the invention to provide a kind of portable detector of tiny sampler.
It is a further object of the present invention to provide detect tiny sampler using described device(Such as worm's ovum in urine)Method.
The above-mentioned purpose of the present invention is to give realization by the following technical programs:
A kind of portable detector of tiny sampler, including fluid box 1, analytic unit 2 and bearing 3;The fluid box 1 is pushed up Portion is provided with injection port 101, and middle part is provided with observation chamber 103, and bottom is provided with waste liquid chamber 105, injection port 101, observation chamber Room 103 and waste liquid chamber 105 are sequentially connected logical by sample introduction runner 102;Tiny sampler filtering is provided with the observation chamber 103 Component 104;The fluid box 1 is provided with peep hole 107 at the position just to observing chamber 103;The analytic unit 2 be with The analytic unit for gathering and/or handling image of camera(Such as smart mobile phone);Analytic unit 2 and bearing 3 are respectively placed at On the analytic unit seat 302 and fluid cassette holder 301 set gradually before and after on bearing 3, the camera of analytic unit 2 is just to peep hole Optical amplifier component is provided between 107, and the camera and peep hole 107.
The optical amplifier component can be arranged on the dividing plate between fluid cassette holder 301 and analytic unit seat 302(Now The height of dividing plate is higher than relevant position on camera and peep hole, dividing plate and is provided with opening, and optical amplifier component is arranged on opening Place), can also be arranged on the outside of camera or on peep hole 107(Now the height of dividing plate is less than camera and peep hole).
Tiny sampler filter assemblies 104 are provided with the observation chamber 103 of the present invention, prevent tiny sampler from by stream Road, and liquid and other impurities can pass through runner;Camera, optical amplifier component, peep hole 107 and the observation of analytic unit 2 Chamber 103 is point-blank;The camera of analytic unit 2 coordinates optical amplifier component to photograph in observation chamber 103 The tiny sampler being trapped, the detection of tiny sampler is realized according to the graphical analysis photographed.
In the said apparatus of the present invention, analysis software is additionally provided with analytic unit 2, the software can obtain micro- in image The information of small sample and the type for analyzing tiny sampler.Specifically, a variety of post is written with by taking parasite egg as an example, in software The morphological feature and image of infested worm's ovum, after mobile phone collects worm's ovum image, software is analysed and compared to image, is passed through Worm's ovum progress multiple alignment with being write in software, determines the type of worm's ovum.
During using the present apparatus, the tiny sampler that liquid is injected in observation chamber 103, liquid by sample introduction runner 102 is by mistake Filter component 104 is trapped in observation chamber 103, and liquid is then flowed into waste liquid chamber 105 by runner.Then analytic unit 2 is used Obtain the image of tiny sampler and analyze and determine its type.
Preferably, the port aperture of the tiny sampler filter assemblies 104 is less than tiny sampler to be detected.
Furthermore it is preferred that the tiny sampler filter assemblies 104 are the port size runner grid more smaller than tiny sampler; The runner grid are multiple equiform short runners, and the upper end of each short runner is in funnel-form.
Preferably, the tiny sampler filter assemblies 104 can also be the smaller screen pack of aperture ratio tiny sampler.
The design of the bearing 3 makes detection process simpler, convenient, reduces manpower and materials;Preferably, the bearing 3 For approximate right-angled trapezium block.
Preferably, it is provided with pulsometer at the injection port 101;When liquid is because of the reason such as the aperture of sample introduction runner 102 is too small And when being had any problem using gravity entrance observation chamber, liquid can more swimmingly be squeezed into by pulsometer and observed in chamber.
Preferably, the analytic unit 2 can be the smart mobile phone with corresponding function, tablet personal computer, video camera etc..
Preferably, it is additionally provided with the venting flow path 106 being connected with waste liquid chamber 105 in the fluid box 1;Fluid box can be made Inside and outside air pressure keeps balance.
It is highly preferred that the gas outlet of the venting flow path 106 is arranged at the top of fluid box.
Preferably, the sample introduction runner 102 is bending.
Preferably, the peep hole 107 is more bigger than observation chamber 103, is easy to observe whole chamber.
The method of tiny sampler, comprises the following steps in a kind of utilization said apparatus detection liquid:
S1. toward liquid of the addition containing tiny sampler to be detected in injection port 101, liquid is injected into sight by sample introduction runner 102 Examine in chamber 103;
S2. the tiny sampler in liquid is intercepted at the top of filter assemblies 104 in observation chamber 103, and liquid then passes through runner Flow into waste liquid chamber 105;
S3. the image of the tiny sampler of the collection of analytic unit 2 retention, and analyzing image, determines tiny sampler type.
Preferably, step S1 is liquid is injected into sight by way of external world's pressurization toward addition liquid in injection port 101 Examine in chamber;Preferably syringe is injected.
The present invention device be applied to various tiny samplers detection, including various micro-organisms, biological tissue and Particulate material.The size dimension of specific device, and filter assemblies 104 aperture(Openings of sizes)According to small sample to be detected The size of product is reasonably adjusted.
Exemplified by detecting the parasite egg in urine, a kind of preferred operable mode, the use of said apparatus are used as Method specifically includes following steps:
Urine specimen is injected into sample introduction runner 102 by the injection port 101 and pulsometer on fluid box 1 first, Ran Houfeng Close injection port.Press pulsometer so that the urine specimen in sample introduction runner 102 is flowed into observation chamber 103, observes chamber There are multiple short runners in 103, form runner grid 104.Urine enters after observation chamber 103, big due to the upper port of runner grid 104 It is small more smaller than worm's ovum in urine, therefore obstructed worm's ovum so that worm's ovum is stuck on runner grid 104, and other liquid then pass through stream Gate 104 is flowed into waste liquid chamber 105, as shown in Figure 5.The venting flow path 106 connected with waste liquid chamber 105 may be such that fluid box 1 Interior air pressure keeps balance, and urine specimen can smoothly flow into observation chamber 103 and waste liquid chamber 105.By 3 points of fluid box 1 and mobile phone It An Fang not be fixed in the mobile phone seat 302 and fluid cassette holder 301 in bearing 3 as shown in Figure 4, the camera of mobile phone 3 can pass through The worm's ovum in 107 pairs of observation chambers 103 of peep hole in camera hole 303 and the shell of fluid box 1 on dividing plate carries out IMAQ, Worm's ovum analysis inspection software on mobile phone can be analyzed image, finally judge the worm's ovum type in urine specimen.
The sample introduction requirement of apparatus of the present invention is impurity(Especially size is more than or equal to the impurity of tiny sampler to be detected)To the greatest extent The few liquid of amount, when detection object is not, it is necessary to do corresponding pre-treatment when worm's ovum or sample are not urines.
Compared with prior art, the invention has the advantages that:
The invention provides a kind of tiny sampler(Such as parasite egg in urine)Portable detector, described device can exempt from The complex operations such as artificial smear, micro- sem observation are gone, realize that the type of the quickly and easily tiny sampler such as worm's ovum judges and detected; It is not only simple in structure, it is with low cost, loss and false drop rate can be also substantially reduced, and the workload of operator is reduced, it is applicable Property extensively, with larger application prospect.
Brief description of the drawings
The structure assembling schematic diagram of the portable detector of worm's ovum in the urine of Fig. 1 embodiments 1.
The side view of the portable detector of worm's ovum in the urine of Fig. 2 embodiments 1.
In the urine of Fig. 3 embodiments 1 in the portable detector of worm's ovum fluid box profile.
In the urine of Fig. 4 embodiments 1 in the portable detector of worm's ovum bearing structural representation.
Fig. 5 embodiments 1 observe chamber structure schematic diagram.
Caption:1- fluid boxes;2- analytic units(Mobile phone);3- bearings;101- injection ports;102- sample introduction runners;103- is seen Examine chamber;104- tiny sampler filter assemblies(Runner grid);105- waste liquid chambers;106- venting flow paths;107- peep holes;301- flows Body cassette holder;302- mobile phone seats;303- camera holes.
Embodiment
The present invention is further illustrated below in conjunction with the drawings and specific embodiments.Wherein, accompanying drawing is given for example only type explanation, What is represented is only schematic diagram, rather than pictorial diagram, it is impossible to be interpreted as the limitation to this patent;In order to which the reality of the present invention is better described Example is applied, some parts of accompanying drawing have omission, zoomed in or out, and do not represent the size of actual product.
The portable detector of worm's ovum in a kind of urine of embodiment 1
The present embodiment by taking the detection of parasite egg in urine as an example, illustrate the present invention tiny sampler detection means structure and Application method.
The portable detector of worm's ovum in a kind of urine, including worm fluid box 1, mobile phone 2 and bearing 3;The worm fluid The top of box 1, which is provided with injection port 101, injection port 101, is provided with small pneumatic pump, and middle part is provided with observation chamber in fluid box 103, bottom is provided with waste liquid chamber 105, injection port 101, observation chamber 103 and the three of waste liquid chamber 105 by sample introduction runner 102 according to It is secondary to be connected;The port size runner grid 104 more smaller than worm's ovum are provided with the observation chamber 103;The fluid box 1 is just right Peep hole 107 is provided with the position for observing chamber 103;The mobile phone 2 is with IMAQ and worm's ovum analysis inspection software Smart mobile phone;The bearing 3 is provided with the fluid cassette holder 301 and mobile phone seat 302 for laying fluid box and mobile phone respectively;Mobile phone seat 302 before fluid cassette holder 301, and both are parallel to each other, just to seeing on the dividing plate between fluid cassette holder 301 and mobile phone seat 302 It is provided with the position for examining hole 107 and the size identical camera hole 303 of peep hole 107;Optics is provided with the camera hole to put Lens group greatly.
The morphological feature and image of a variety of urine worm's ovums are written with the worm's ovum analysis inspection software, when mobile phone collection To after worm's ovum image, worm's ovum inspection software is analysed and compared to image, and multiple ratio is carried out by the worm's ovum with being write in software It is right, determine the type of worm's ovum;In addition, the differentiation of the worm's ovum image information progress worm's ovum type of acquisition also can be directly observed, and nothing Software analysis need to be passed through.
Meanwhile, the venting flow path 106 being connected with waste liquid chamber 105 is provided with the fluid box, its aeration vent is set At the top of fluid box, fluid box inner and outer air pressure can be made to keep balance.
In addition, the sample introduction runner 102 is bending.
The peep hole 107 is more bigger than observation chamber 103, is easy to observe whole chamber.
The mobile phone camera, camera hole 303, peep hole 107 and observation chamber 103 it is same perpendicular to fluid cassette holder and On the straight line of mobile phone seat.
The bearing 3 is approximate right-angled trapezium block.
During using apparatus of the present invention, urine specimen is injected into by the injection port 101 and pulsometer on fluid box 1 first In sample introduction runner 102, injection port is then closed.Press pulsometer so that the urine specimen in sample introduction runner 102 is flowed into observation In chamber 103, there are multiple short runners in observation chamber 103, form runner grid 104.Urine enters after observation chamber 103, due to The size of the upper port of runner grid 104 is more smaller than worm's ovum in urine, therefore has obstructed worm's ovum so that worm's ovum is stuck on runner grid 104, And other liquid are then flowed into waste liquid chamber 105 by runner grid 104, as shown in Figure 5.The vent flow connected with waste liquid chamber 105 Road 106 may be such that the air pressure in fluid box 1 keeps balance, and urine specimen can smoothly flow into observation chamber 103 and waste liquid chamber 105. Fluid box 1 and mobile phone 3 are laid respectively in the mobile phone seat 302 and fluid cassette holder 301 being fixed in bearing 3 as shown in Figure 4, hand The camera of machine 3 can be by 107 pairs of observation chambers 103 of peep hole in the camera hole 303 on dividing plate and the shell of fluid box 1 The worm's ovum inspection software that worm's ovum is carried out on IMAQ, mobile phone can be analyzed image, finally judged in urine specimen Worm's ovum type.
In addition, it is not urine to work as sample, but, it is necessary to which first pretreatment is processed into containing worm when excrement or other samples The liquid of ovum, it is ensured that try one's best less or do not contain the impurity that size is more than or equal to worm's ovum in liquid.
Detection means of the present invention can remove artificial smear operation from, simple in construction, facilitate testing staff to carry, it is easy to scene inspection Survey, it is with low cost, the loss and false drop rate of worm's ovum in urine can be substantially reduced, and reduce artificial workload.

Claims (8)

1. a kind of portable detector of tiny sampler, it is characterised in that including fluid box(1), analytic unit(2)And bearing (3);The fluid box(1)Top is provided with injection port(101), middle part is provided with observation chamber(103), bottom is provided with waste liquid Chamber(105), injection port(101), observation chamber(103)With waste liquid chamber(105)Pass through sample introduction runner(102)It is sequentially connected logical;Institute State observation chamber(103)In be provided with tiny sampler filter assemblies(104);The fluid box(1)Just to observation chamber on box body (103)Position at be provided with peep hole(107);The analytic unit(2)For gathering with camera and/or processing figure The analytic unit of picture;Analytic unit(2)And bearing(3)It is respectively placed at bearing(3)The analytic unit seat set gradually before and after upper (302)With fluid cassette holder(301)On, analytic unit(2)Camera just to peep hole(107), and the camera and observation Hole(107)Between be provided with optical amplifier component.
2. portable detector according to claim 1, it is characterised in that the tiny sampler filter assemblies(104) Port aperture be slightly less than tiny sampler to be detected.
3. portable detector according to claim 1, it is characterised in that the optical amplifier component is arranged on fluid Cassette holder(301)With analytic unit seat(302)Between dividing plate on, on the outside of camera or peep hole(107)On.
4. portable detector according to claim 1, it is characterised in that the injection port(101)Place is provided with gas Press pump.
5. portable detector according to claim 1, it is characterised in that be provided with the fluid box and waste liquid chamber (105)The venting flow path being connected(106).
6. the portable detector of worm's ovum in urine according to claim 5, it is characterised in that the venting flow path (106)Gas outlet be arranged at the top of fluid box.
7. portable detector according to claim 1, it is characterised in that the analytic unit(2)In be additionally provided with Analysis software, the software can obtain the information of tiny sampler in image and analyze the type of tiny sampler.
8. the method that the device described in a kind of any one of utilization claim 1~7 detects tiny sampler, it is characterised in that including Following steps:
S1. toward injection port(101)Middle to add the liquid containing tiny sampler to be detected, liquid passes through sample introduction runner(102)Injection To observation chamber(103)In;
S2. the tiny sampler in liquid is intercepted at observation chamber(103)In filter assemblies(104)Top, liquid then passes through Runner flows into waste liquid chamber(105)In;
S3. analytic unit(2)The image of the tiny sampler of retention is gathered, the detection of tiny sampler is realized according to graphical analysis.
CN201710529135.0A 2017-07-01 2017-07-01 The portable detector and method of a kind of parasite egg Pending CN107271440A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113207844A (en) * 2021-03-19 2021-08-06 温州医科大学 Parasite protection device for IVC system

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CN106442962A (en) * 2016-09-12 2017-02-22 北京纳迅科技股份有限公司 POCT instant detection device
CN106769327A (en) * 2017-01-23 2017-05-31 湖南省天骑医学新技术股份有限公司 A kind of Double-layered cup and the method for inspection for collecting Helminthic Eggs
CN207366452U (en) * 2017-07-01 2018-05-15 中山大学 A kind of portable detector of parasite egg

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WO1986000704A1 (en) * 1984-07-17 1986-01-30 International Health Services Method and apparatus for filtering particulate matter from fluids of biomedical interest and examining same
US5681529A (en) * 1994-08-25 1997-10-28 Nihon Medi-Physics Co., Ltd. Biological fluid analyzing device
WO2001032828A2 (en) * 1999-10-29 2001-05-10 Pall Corporation Biological fluid processing
US20040253143A1 (en) * 2003-06-16 2004-12-16 Yokogawa Electric Corporation Method for processing waste liquid in cartridges and a chemical reaction cartridge applying the method
US20140367317A1 (en) * 2012-01-24 2014-12-18 Koninklijke Philips N.V. Analysis cartridge with filter unit
KR20130130443A (en) * 2012-05-22 2013-12-02 성기용 Semen analysis system using portable device
WO2014142754A1 (en) * 2013-03-13 2014-09-18 Cellsievo Pte Ltd Microsieve
CN204945143U (en) * 2015-08-31 2016-01-06 福州大学 A kind of biochemical test bar pick-up unit being applicable to mobile terminal
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113207844A (en) * 2021-03-19 2021-08-06 温州医科大学 Parasite protection device for IVC system

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