CN113912458A - Method for preparing micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms - Google Patents
Method for preparing micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms Download PDFInfo
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Abstract
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using a super-selenium-resistant microorganism, which comprises the steps of carrying out amplification culture on bacillus subtilis, bacillus licheniformis and the like which have super-selenium-resistant capability and can reduce selenium by using a specific culture medium, transferring a proper amount of fresh bacterial liquid into another liquid culture medium, adding a certain amount of sodium selenite, terminating the culture until the culture medium is bright red, uniformly mixing with a certain amount of humic acid, biochemical fulvic acid and organic matters, and drying. The invention has the advantages of simple technology, easily obtained materials, convenient application, lasting selenium increase, feasibility, practicability and popularization value.
Description
Technical Field
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using a super-selenium-resistant microorganism.
Background
Selenium is one of 14 essential trace elements of the human body, is also a dietary nutrient which needs to be taken every day and must be supplemented by diet.
The trace element selenium has multiple functions of oxidation resistance, cancer resistance, virus resistance, immunity improvement and the like, has little content in a human body, is only about 14 mg, but has irreplaceable effect, is known as 'vital fire species', 'cancer king', 'heart guard', 'liver guard', 'insulin in trace elements' and the like, is an indispensable nutrient for the human body, and is an important vital nutrient equal to biochemical substances such as protein, vitamin, beta-carotene and the like.
The earth surface reserves of selenium are rare and extremely unbalanced in distribution, more than 40 countries in the world lack selenium, wherein the whole situation of Finland and New Zealand lack selenium is a global cardiovascular disease high-incidence area, the government adopts food-intake to establish the selenium content requirement, the domestic agricultural products and the livestock industry adopt mandatory selenium supplement measures, the selenium content standard is reached by applying selenium fertilizer and selenium feed, and the cardiovascular disease rate is at the lowest level in the world after being insisted for decades.
Selenium is found for more than 200 years, and numerous researches at home and abroad prove that the selenium is directly related to the occurrence and development of more than 40 diseases and indirectly related to hundreds of diseases from aspects of epidemiology, nutrition, preventive medicine, clinical medicine and the like. The extreme importance of selenium supplementation to human health and quality of life.
Under the environmental background of selenium deficiency of soil, the average selenium content in the soil is only 0.2mg/kg, the low-selenium soil is only about 0.03mg/kg, and the selenium-enriched agricultural products are only produced by increasing the selenium. The aim is mainly achieved by the following method:
1. adopting sodium selenite root irrigation or foliage spraying: the fertilizer has certain selenium increasing effect on agricultural products, but has larger side effect, namely, soil is acidified; secondly, the environment is polluted, especially the water source is polluted; thirdly, the spraying is needed for several times, the labor intensity is high, the cost is high, and the safety of people and livestock is threatened; fourthly, the bad dosage and method are easy to cause the loss due to the disorganization of roots or seedling burning.
2. The sodium selenite is chelated with fulvic acid, or chemical organic selenium (potassium selenocyanate) or selenocarrageenan is directly prepared into a foliar fertilizer for spraying, so that the same risks and problems as the above exist.
3. The selenium-containing rock is crushed and applied to soil, the problems of environment, cost and the like also exist, and the selenium-containing rock is protected according to law and mining is prohibited.
4. The solid fertilizer prepared by fermenting crop straws, animal wastes and sodium selenite is too high in cost, the application amount per mu needs to reach 2000kg, and resources and manpower are unrealistic.
The measures of the method have certain defects and problems, and a plurality of research reports exist in China and are not described any more.
The selenium fertilizer produced by the microorganisms in China is produced by utilizing the fermentation function of the selenium fertilizer, and the microorganisms are not directly used as slow-release raw materials. In addition, the general microorganisms do not have the selenium resistance, most microorganisms cannot survive under the condition of 200 mu g/ml selenium concentration, the selenium resistance of individual saccharomycetes is 300 mu g/ml, but the selenium resistance can reach the concentration of 2000 mu g/ml selenium through chemical induction or long-term domestication, thereby limiting the development and utilization. However, some microorganisms (mainly bacillus subtilis and bacillus licheniformis) growing on the Enshi selenium ore bed in Hubei can resist selenium of more than 50000 mu g/ml at most, because the microorganisms grow for a long time under the environment of high selenium and have genetic variation, the microorganisms can resist the high selenium and can reduce the selenium into red elemental selenium with the nanometer level (160-180nm) (Peng \31066;, separation, screening and identification of 3 strains of ultra-selenium-resistant microorganisms, and trace element and health research magazines 2012, 29 (3): 4), so the ultra-selenium-resistant microorganisms become the material basis of the invention.
Disclosure of Invention
The invention aims to provide a method for preparing a microbial slow-release organic selenium fertilizer by using a selenium-super-resistant microorganism.
The invention provides a technical process for preparing a micro-ecological slow-release organic selenium fertilizer by using a super-selenium-resistant microorganism. The content is that bacillus subtilis and bacillus licheniformis which have super selenium-resistant capability and can reduce selenium are expanded and cultured by a specific culture medium, a proper amount of fresh bacterial liquid is transferred to another liquid culture medium, a certain amount of sodium selenite is added, the culture is stopped until the culture medium is bright red (selenium is reduced into red biological simple substance selenium), and the culture medium is evenly mixed with a certain amount of humic acid, biochemical fulvic acid and organic matter and dried.
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super selenium-resistant microorganisms, which is characterized by comprising the following steps: a. and (3) secondary enrichment culture: collecting leaf mold and black soil on selenium ore bed, enriching for 6 hr at 36 deg.C in common broth culture medium, transferring the enriched liquid into nutrient broth culture medium containing 500 μ g/ml selenium, and culturing for 6 hr at 36 deg.C; b. inoculation: inoculating the bacterial liquid of the second enrichment culture on a nutrient agar culture medium containing 2000 mu g/ml selenium, and culturing for 24 hours at 36 ℃ to ensure that the culture medium turns deep red; c. seed transformation: transferring the deep red colony into a special liquid culture medium to culture for 24 hours at 36 ℃; d. drying and granulating: adding the seed conversion solution into 1500g of solid organic fertilizer, uniformly mixing, drying and granulating for later use.
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super selenium-resistant microorganisms, which is characterized by comprising the following steps: a. and (3) secondary enrichment culture: collecting leaf mold and black soil on selenium ore bed, enriching for 6 hr at 36 deg.C in common broth culture medium, transferring the enriched liquid into nutrient broth culture medium containing 500 μ g/ml selenium, culturing for 6 hr at 36 deg.C, and eliminating mixed bacteria; b. inoculation: inoculating the bacterial liquid of the second enrichment culture on a nutrient agar culture medium containing 2000 mug/ml selenium, and culturing for 24 hours at 36 ℃ to ensure that the culture medium becomes dark red, which indicates that the selenium in the culture medium is reduced into biological simple substance selenium by bacteria; c. seed transformation: transferring the deep red colony into a special liquid culture medium to be cultured for 24 hours at 36 ℃, wherein the special liquid culture medium is prepared by taking 1-3g of agarose, 3-5g of tryptone, 10-15g of yeast, 2-6g of dipotassium phosphate, 1-3g of glucose, 3-5g of beef extract, 5g of sodium chloride, 10-15g of brown sugar, 10-15g of biochemical fulvic acid and 6-10mg of manganese sulfate, adding clean water to 1000ml and keeping the pH value at 7.2; d. drying and granulating: adding the seed transfer solution into 1500g of solid organic fertilizer, uniformly mixing, drying and granulating for later use, wherein the solid organic fertilizer is prepared by uniformly mixing 500g of humic acid, biochemical fulvic acid and organic matter; the organic matter is peanut cake powder, soybean cake powder or other similar materials which are mixed according to the proportion of 1:1: 1.
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super selenium-resistant microorganisms, which is characterized in that 100g of root is applied to each plant, the surface layer is covered by soil, and the root can be applied singly or simultaneously with other fertilizers.
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms, which comprises the steps of collecting leaf mold and black soil samples on a selenium ore bed, enriching for 6 hours in a common broth culture medium at 36 ℃, transferring enriched liquid into a nutrient broth culture medium added with 500 mu g/ml of selenium, continuously culturing for 6 hours at 36 ℃, and eliminating mixed bacteria.
Inoculating the bacterial liquid of the second enrichment culture on a nutrient agar culture medium containing 2000 mu g/ml selenium, and culturing at 36 ℃ for 24 hours, wherein the culture medium turns deep red, which indicates that the selenium in the culture medium is reduced into biological elemental selenium by bacteria.
Transferring the deep red colony into a specific special liquid culture medium (agarose 1-3g/L, tryptone 3-5g/L, yeast 10-15g/L, dipotassium hydrogen phosphate 2-6g/L, glucose 1-3g/L, beef extract 3-5g/L, sodium chloride 5g/L, brown sugar 10-15g/L, biochemical fulvic acid 10-15g/L, manganese sulfate 6-10mg/L, clean water 1000ml, PH7.2), culturing at 36 ℃ for 24 hours, uniformly mixing humic acid, biochemical fulvic acid, organic matter (peanut cake powder, soybean cake powder or other similar materials in a ratio of 1:1: 1), drying, granulating for standby, applying 100g to roots of each plant, covering the surface layer with soil, and applying only, it can also be applied simultaneously with other fertilizers.
The invention discloses a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms, which is innovative in that: 1. the special selenium-resistant microorganisms (bacillus subtilis and bacillus licheniformis) are used as the slow release selenium source, a solution is provided for the slow release of organic selenium organisms, the selenium source is novel, and the method is initiated at home; 2. the method has the advantages that most bacteria cannot survive and reproduce under high selenium concentration, secondary selenium resistance screening is carried out on collected samples, complex identification such as culture characteristics, biological properties and biochemical characteristics is not needed, and the method is scientific, practical and innovative; 3. the developed special high-selenium culture medium is suitable for growth and propagation of bacillus subtilis and bacillus licheniformis and establishment of a soil microecosystem with selenium resistance, and is initiated at home.
The invention utilizes the super selenium-resistant microorganisms to prepare the micro-ecological slow-release organic selenium fertilizer, and the theoretical basis of the technology is as follows: 1. the super selenium-resistant capability of bacillus subtilis and bacillus licheniformis is utilized, and the method is the basis for the research and development of the micro-ecological selenium fertilizer; 2. the bacillus subtilis and the bacillus licheniformis can grow, reproduce and continuously passage under severe environments such as high temperature and the like, and the capability of reducing selenium is also the basis for the research and development of the micro-ecological selenium fertilizer; 3. the bacillus subtilis and the bacillus licheniformis absorb a large amount of selenium to enter the body, so that a large amount of selenium is kept from being lost, part of selenium is absorbed by plants after the bacteria are disintegrated, and part of selenium is re-absorbed by new generation bacteria, thereby laying a foundation for researching and developing the slow release selenium fertilizer. Therefore, after the slow-release micro-ecological organic selenium fertilizer provided by the invention is applied to soil, plants firstly absorb nano-scale (160-. 4. The humic acid, the biochemical fulvic acid and the organic matters are used as not only fertilizers but also microbial culture materials, and the performance of promoting the activity of the microorganisms is utilized, so that a material basis is provided for the long-term propagation of the microorganisms in soil, the growth of the bacteria needed by the heart, such as phosphate solubilizing bacteria, potassium solubilizing bacteria, nitrogen fixing bacteria and the like, and the establishment of the micro-ecology. The combination of all factors can not only provide organic selenium source and achieve the purpose of slow release, but also improve the soil performance, increase the soil fertility and promote the growth of crops. 5. The invention does not utilize chemical slow release agent, but utilizes the super selenium-resistant and reproductive performance of specific microorganism to play a slow release role, the developed special high-selenium liquid culture medium has strong pertinence and good culture effect, a microecological system which is favorable for improving the soil and the growth of plants can be established in the soil, the slow release microecological selenium supplement can last for 3 years, and the invention has creativity and advancement.
The invention discloses a technology for preparing a micro-ecological slow-release organic selenium fertilizer by using a super-selenium-resistant microorganism, provides a special technology for preparing the micro-ecological slow-release organic selenium fertilizer by using the super-selenium-resistant microorganism, and provides a biological solution for the slow release of organic selenium.
The invention discloses a technology for preparing a micro-ecological slow-release organic selenium fertilizer by using a super-selenium-resistant microorganism, and provides a special high-selenium culture medium which is suitable for growth and propagation of super-selenium-resistant bacillus subtilis and bacillus licheniformis and establishment of an excellent soil micro-ecological system.
The invention has the advantages of simple technology, easily obtained materials, convenient application, lasting selenium increase, feasibility, practicability and popularization value.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms, which comprises the following steps: a. and (3) secondary enrichment culture: collecting leaf mold and black soil on selenium ore bed, enriching for 6 hr at 36 deg.C in common broth culture medium, transferring the enriched liquid into nutrient broth culture medium containing 500 μ g/ml selenium, culturing for 6 hr at 36 deg.C, and eliminating mixed bacteria; b. inoculation: inoculating the bacterial liquid of the second enrichment culture on a nutrient agar culture medium containing 2000 mug/ml selenium, and culturing for 24 hours at 36 ℃ to ensure that the culture medium becomes dark red, which indicates that the selenium in the culture medium is reduced into biological simple substance selenium by bacteria; c. seed transformation: transferring the deep red colony into a special liquid culture medium to be cultured for 24 hours at 36 ℃, wherein the special liquid culture medium is prepared by taking 1-3g of agarose, 3-5g of tryptone, 10-15g of yeast, 2-6g of dipotassium phosphate, 1-3g of glucose, 3-5g of beef extract, 5g of sodium chloride, 10-15g of brown sugar, 10-15g of biochemical fulvic acid and 6-10mg of manganese sulfate, adding clean water to 1000ml and keeping the pH value at 7.2; d. drying and granulating: adding the seed transfer solution into 1500g of solid organic fertilizer, uniformly mixing, drying and granulating for later use, wherein the solid organic fertilizer is prepared by uniformly mixing 500g of humic acid, biochemical fulvic acid and organic matter; the organic matter is peanut cake powder, soybean cake powder or other similar materials which are mixed according to the proportion of 1:1: 1. 100g of fertilizer is applied to roots of each plant, the surface layer is covered by soil, and the fertilizer can be applied singly or simultaneously with other fertilizers.
Example 2
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms, which comprises the steps of inoculating a secondary enriched bacterial solution into a specific culture medium containing 1g/L of agarose, 3g/L of tryptone, 10g/L of yeast, 6g/L of dipotassium hydrogen phosphate, 2g/L of glucose, 3g/L of beef extract, 5g/L of sodium chloride, 10g/L of brown sugar, 15g/L of biochemical fulvic acid, 6mg/L of manganese sulfate, adding 1000ml of sodium selenite (analytically pure) clean water and pH7.2, culturing for 24 hours at 36 ℃, uniformly mixing 500g of humic acid, 500g of biochemical fulvic acid and 500g of organic matters (soybean cake powder and rapeseed cake 1:1 mixture), and drying for later use.
Example 3
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms, which comprises the steps of inoculating a secondary enriched bacterial solution into a specific culture medium containing 3g/L agarose, 5g/L tryptone, 15g/L yeast, 2g/L dipotassium hydrogen phosphate, 3g/L glucose, 5g/L beef extract, 5g/L sodium chloride, 15g/L brown sugar, 10g/L biochemical fulvic acid and 10mg/L manganese sulfate, culturing for 24 hours at 36 ℃, uniformly mixing 500g humic acid, 500g biochemical fulvic acid and 500g organic matters (soybean cake powder and rapeseed cake 1:1 mixture), and drying for later use.
Example 4
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using a super-selenium-resistant microorganism, and a potted tomato test
1. Inoculating the bacterial liquid subjected to secondary enrichment in the technical scheme to a specific culture medium containing 1g/L of agarose, 3g/L of tryptone, 10g/L of yeast, 6g/L of dipotassium hydrogen phosphate, 2g/L of glucose, 3g/L of beef extract, 5g/L of sodium chloride, 10g/L of brown sugar, 15g/L of biochemical fulvic acid, 6mg/L of manganese sulfate and sodium selenite (analytically pure) water, adding the mixture into 1000ml of the specific culture medium with pH of 7.2, culturing the mixture at 36 ℃ for 24 hours, uniformly mixing 500g of humic acid, 500g of biochemical fulvic acid and 500g of organic matters (soybean cake powder and rapeseed cake 1:1 mixture), and drying the mixture for later use.
Collecting field surface soil, performing potted tomato tests, setting 5 pots as one group, applying 100g of root to each tomato plant, using the same field soil as a control group, only applying no biological slow-release organic selenium fertilizer, and measuring the selenium content of the tomatoes during maturation. Picking a tomato with the same size from each tomato plant, merging and pulping, taking out about 50g of pulp, drying, weighing 1.0000g of pulp, and measuring selenium according to the GB5009.93 method.
The results were: the selenium content of the test group is 62.3 mug/100 g, the selenium content of the control group is 11.7 mug/100 g, the selenium content of the test group is 5.32 times of that of the control group, and the selenium increasing effect is very obvious. The total number of bacteria in the soil was determined to be 2.43 hundred million/g.
The potted soil after harvesting the tomatoes is placed under natural conditions, and weeds are removed timely for the next year of successive tests.
2. The soil cultivated in a pot in the first year is subjected to a double pot, water and nutrients are supplemented, tomato seedlings are planted, the selenium content of the tomatoes is determined by the method after the tomatoes are mature, and the result is that: the content of selenium in the test group is 4.9 times that in the control group. The selenium content in the test group decreased by 28.4% in the second year compared to the first year, approximately 30%, similar to the same other test results. The total number of bacteria in the soil is measured to be 2.01 hundred million/g, and the total number of the bacteria is reduced by 17.28 percent because the soil is stored for a long time and is not beneficial to the activity and the growth of the bacteria.
3. And (5) turning over the soil planted in the second year, adding water and nutrients, and using the soil for the test in the third year. The selenium content of the test group is 12.4 mug/100 g, the selenium content of the control group is 8.2 mug/100 g, and the selenium content of the test group is 1.5 times of that of the control group. The third year is reduced by 72.2 percent compared with the second year, and the reduction range is more than one time of the second year. The selenium content of the tomatoes just reaches the standard requirement of DBS 42/002-2014. The total number of bacteria in the soil is measured to be 1.03 hundred million/g, which is reduced by more than half compared with the first year, the effective period of the micro-ecological slow-release organic selenium fertilizer can reach 3 years for selenium supplement, and about 50g of the product can be applied in the second year if selenium-rich tomatoes with stable selenium content are produced.
Example 5
The invention relates to a method for preparing a micro-ecological slow-release organic selenium fertilizer by using a super-selenium-resistant microorganism, and a hot pepper planting test in a field
1. Inoculating the bacterial liquid subjected to secondary enrichment in the technical scheme to a specific culture medium containing 3g/L of agarose, 5g/L of tryptone, 15g/L of yeast, 2g/L of dipotassium hydrogen phosphate, 3g/L of glucose, 5g/L of beef extract, 5g/L of sodium chloride, 15g/L of brown sugar, 10g/L of biochemical fulvic acid and 10mg/L of manganese sulfate, culturing for 24 hours at 36 ℃, uniformly mixing 500g of humic acid, 500g of biochemical fulvic acid and 500g of organic matter (soybean cake powder and rapeseed cake 1:1 mixture), and drying for later use.
Selecting suitable field, and dividing into two parts, one part is used as test group, and the other part is used as control group. The pepper is planted by a conventional farming method, and each plant of root is applied with 100g of micro-ecological slow-release organic selenium fertilizer and covered with soil. After the pepper turns red and matures, sampling according to five points in four corners, picking 3 mature peppers per root, drying, crushing together, weighing 2.0000g, and measuring selenium according to the method specified in GB5009.93, wherein the selenium content of the test group is 46.23 mug/100 g, the selenium content of the control group is 8.94 mug/100 g, and the test group is 5.17 times of the control group and is consistent with the potted tomato. The total number of bacteria in the soil was determined to be 2.15 hundred million/g. After the pepper is harvested, the land is left unused until the next year for secondary cultivation.
2. Loosening soil again and weeding the land planted with the peppers in the first year, planting the peppers, and performing conventional management. The pepper is picked in the mode of the first year after being ripe, and the selenium is measured by sample preparation. As a result, the content of the selenium in the test group is reduced by 53.5 mu g/100g compared with that in the control group, the content of the selenium in the test group is reduced by 9.2 mu g/100g compared with that in the control group by 2.34 times, and the content of the selenium in the test group is reduced by 53.5% in the first year and is greatly higher than the content of the selenium in the potted tomato by 28.4% in the second year, which indicates that certain loss is brought to the microbial selenium in the field due to the effects of soil turning, dilution and loss. The total number of bacteria in the soil is measured to be 1.78 hundred million/g, which is 16.3 percent lower than that in the first year, and is consistent with the situation of pepper pot culture. The micro-ecological stability of the soil is better. After the pepper is harvested, the land is idle again to be cultivated again in the next year.
3. Loosening soil of the land planted with the peppers in the next year, and performing conventional management. The pepper is picked in the mode of the first year after being ripe, and the selenium is measured by sample preparation. As a result, the selenium content of the test group is 9.5 mug/100 g, the selenium content of the control group is 8.2 mug/100 g, the test group is reduced by 1.16 times compared with the control group, the selenium content of the test group is reduced by 55.8% in the second year, the reduction range is basically the same as that of the second year, but the reduction range is lower than that of the third year by 72.2% compared with the potted tomato test, and the reduction of the selenium content in the potting soil and the absorption of a large amount of plants in the potting soil can be related to the reduction of the selenium content in the third year. The total number of bacteria in the soil is measured to be 1.25 hundred million/g, which is 29.77 percent lower than that in the first year. The total decrease in bacteria in the soil may be related to other environmental factors in the soil. The selenium content of the third year pepper is 9.5 mug/100 g, which is already lower than the requirement of 10 mug/100 g. Therefore, 50g of micro-ecological organic selenium fertilizer should be additionally applied to each stump when the seedlings are planted in the third year.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A method for preparing a micro-ecological slow-release organic selenium fertilizer by using super selenium-resistant microorganisms is characterized by comprising the following steps: a. and (3) secondary enrichment culture: collecting leaf mold and black soil on selenium ore bed, enriching for 6 hr at 36 deg.C in common broth culture medium, transferring the enriched liquid into nutrient broth culture medium containing 500 μ g/ml selenium, and culturing for 6 hr at 36 deg.C; b. inoculation: inoculating the bacterial liquid of the second enrichment culture on a nutrient agar culture medium containing 2000 mu g/ml selenium, and culturing for 24 hours at 36 ℃ to ensure that the culture medium turns deep red; c. seed transformation: transferring the deep red colony into a special liquid culture medium to culture for 24 hours at 36 ℃; d. drying and granulating: adding the seed conversion solution into 1500g of solid organic fertilizer, uniformly mixing, drying and granulating for later use.
2. The method for preparing the microbial slow-release organic selenium fertilizer by using the selenium-super-resistant microorganisms as claimed in claim 1, which is characterized by comprising the following steps of: a. and (3) secondary enrichment culture: collecting leaf mold and black soil on selenium ore bed, enriching for 6 hr at 36 deg.C in common broth culture medium, transferring the enriched liquid into nutrient broth culture medium containing 500 μ g/ml selenium, culturing for 6 hr at 36 deg.C, and eliminating mixed bacteria; b. inoculation: inoculating the bacterial liquid of the second enrichment culture on a nutrient agar culture medium containing 2000 mug/ml selenium, and culturing for 24 hours at 36 ℃ to ensure that the culture medium becomes dark red, which indicates that the selenium in the culture medium is reduced into biological simple substance selenium by bacteria; c. seed transformation: transferring the deep red colony into a special liquid culture medium to be cultured for 24 hours at 36 ℃, wherein the special liquid culture medium is prepared by taking 1-3g of agarose, 3-5g of tryptone, 10-15g of yeast, 2-6g of dipotassium phosphate, 1-3g of glucose, 3-5g of beef extract, 5g of sodium chloride, 10-15g of brown sugar, 10-15g of biochemical fulvic acid and 6-10mg of manganese sulfate, adding clean water to 1000ml and keeping the pH value at 7.2; d. drying and granulating: adding the seed transfer solution into 1500g of solid organic fertilizer, uniformly mixing, drying and granulating for later use, wherein the solid organic fertilizer is prepared by uniformly mixing 500g of humic acid, biochemical fulvic acid and organic matter; the organic matter is peanut cake powder, soybean cake powder or other similar materials which are mixed according to the proportion of 1:1: 1.
3. The method for preparing the microbial slow-release organic selenium fertilizer by using the selenium-super-resistant microorganisms as claimed in claim 1, wherein 100g of the organic selenium fertilizer is applied to roots of each plant, the surface layer of the plant is covered by soil, and the organic selenium fertilizer can be applied singly or simultaneously with other fertilizers.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111236874.3A CN113912458A (en) | 2021-10-24 | 2021-10-24 | Method for preparing micro-ecological slow-release organic selenium fertilizer by using super-selenium-resistant microorganisms |
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| WO2009154234A1 (en) * | 2008-06-18 | 2009-12-23 | 電源開発株式会社 | Novel microorganisms, selenium oxide compound reducing agent, method for reducing and method for removing a selenium oxide compound, and process for producing metallic selenium |
| CN102703513A (en) * | 2012-05-26 | 2012-10-03 | 彭祚全 | Method for preparing red elemental selenium by utilizing super-selenium resistance microorganism |
| JP2014124106A (en) * | 2012-12-25 | 2014-07-07 | Electric Power Dev Co Ltd | New microorganism, selenate compound-reducing agent, method for reducing selenate compound, method for removing selenate compound and method for producing metallic selenium |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
| CN109182128A (en) * | 2018-10-16 | 2019-01-11 | 广西壮族自治区农业科学院农业资源与环境研究所 | A kind of strain culturing method of Efficient Conversion Selenium in Soil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2009154234A1 (en) * | 2008-06-18 | 2009-12-23 | 電源開発株式会社 | Novel microorganisms, selenium oxide compound reducing agent, method for reducing and method for removing a selenium oxide compound, and process for producing metallic selenium |
| CN102703513A (en) * | 2012-05-26 | 2012-10-03 | 彭祚全 | Method for preparing red elemental selenium by utilizing super-selenium resistance microorganism |
| JP2014124106A (en) * | 2012-12-25 | 2014-07-07 | Electric Power Dev Co Ltd | New microorganism, selenate compound-reducing agent, method for reducing selenate compound, method for removing selenate compound and method for producing metallic selenium |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
| CN109182128A (en) * | 2018-10-16 | 2019-01-11 | 广西壮族自治区农业科学院农业资源与环境研究所 | A kind of strain culturing method of Efficient Conversion Selenium in Soil |
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Application publication date: 20220111 |