CN113907382A - Microbial fermentation feed fermentation system - Google Patents
Microbial fermentation feed fermentation system Download PDFInfo
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- CN113907382A CN113907382A CN202111186457.2A CN202111186457A CN113907382A CN 113907382 A CN113907382 A CN 113907382A CN 202111186457 A CN202111186457 A CN 202111186457A CN 113907382 A CN113907382 A CN 113907382A
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- fermentation
- montmorillonite
- base material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23N—MACHINES OR APPARATUS FOR TREATING HARVESTED FRUIT, VEGETABLES OR FLOWER BULBS IN BULK, NOT OTHERWISE PROVIDED FOR; PEELING VEGETABLES OR FRUIT IN BULK; APPARATUS FOR PREPARING ANIMAL FEEDING- STUFFS
- A23N17/00—Apparatus specially adapted for preparing animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to the technical field of fermented feed, and particularly discloses a microbial fermented feed fermentation system, which comprises the following operation steps: the method comprises the following steps: crushing montmorillonite raw materials, crushing the montmorillonite raw materials by 100-200 meshes, then sending the crushed montmorillonite raw materials into an electron beam irradiation box for electron beam irradiation-laser shock wave coupling treatment, and taking out the montmorillonite raw materials for later use after the electron beam irradiation-laser shock wave coupling treatment is finished; step two: then sending the mixture into a pretreatment tank, adding a modification liquid, stirring and mixing at the stirring speed of 100-500r/min for 30-40min to obtain a pre-primary material; step three: and (3) feeding the pre-primary material into an ionization tank for ionization treatment, and then washing, centrifuging and drying after ionization is finished to obtain the montmorillonite base material. The montmorillonite base material obtained by the invention is convenient for subsequent fermentation treatment, and is mixed with the microecological preparation, the microecological preparation is fully dispersed into the montmorillonite base material, so that the fermentation efficiency of the montmorillonite base material and the microecological preparation is improved, and the fermentation efficiency of the montmorillonite base material and the microecological preparation is further improved by the fermentation base material.
Description
Technical Field
The invention relates to the technical field of fermented feed, in particular to a microbial fermented feed fermentation system.
Background
The fermentation system is generally used for microbial fermentation, while the fermentation system for producing the mixed feed additive is used in the production process of the mixed feed additive, and most of the fermented microbes are beneficial bacteria or trace substances; with the rapid development of the breeding industry, the requirements on the feed required by the breeding are higher and higher, and the additives are added into the feed to improve the microbial environment in the livestock and supplement trace elements so as to achieve the purpose of improving the breeding yield and quality, so the requirements on the system for producing the fermentation additives are also higher and higher.
The existing feed fermentation system is simple, montmorillonite exists in the fermentation, the montmorillonite system structure is stable and not easy to disperse, the microorganism aggregation is easy to promote, and the fermentation efficiency is influenced.
Disclosure of Invention
The invention aims to provide a microbial fermented feed fermentation system to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a microbial fermentation feed fermentation system comprises the following operation steps:
the method comprises the following steps: crushing montmorillonite, crushing the montmorillonite by 100-200 meshes, then sending the crushed montmorillonite into an electron beam irradiation box for electron beam irradiation-laser shock wave coupling treatment, and taking out the montmorillonite for later use after the electron beam irradiation-laser shock wave coupling treatment is finished;
step two: then sending the mixture into a pretreatment tank, adding a modification liquid, stirring and mixing at the stirring speed of 100-500r/min for 30-40min to obtain a pre-primary material;
step three: feeding the pre-primary material into an ionization tank for ionization treatment, and then washing, centrifuging and drying after ionization to obtain a montmorillonite base material;
step four: mixing montmorillonite base material, molasses, microecological preparation and fermentation base material according to the weight ratio of 6:2:1:1, stirring at the rotation speed of 50-200r/in for 10-20min to obtain fermentation base material;
step five: homogenizing the fermentation material before feeding into a fermentation tank, and fermenting at 25-35 deg.C for 5-15 hr to obtain fermented feed.
Preferably, the irradiation dose of the electron beam in the electron beam irradiation-laser shock wave coupling treatment is 1-5 MeV.
Preferably, the irradiation dose of the electron beam is 3 MeV.
Preferably, the pulse width during the laser shock wave treatment is 20-30ns, and the laser shock power density is 0.1-0.3GW/cm2The energy is 1-3J, and the treatment time is 1-5 min.
Preferably, the preparation method of the modifying solution comprises the following steps: adding 2-5 parts of sodium chloride into 50-100 parts of water, then adding 5-10 parts of sodium citrate, stirring at the rotation speed of 500r/min for 20-30min at 300-.
Preferably, the microecological agent is one or more of bacillus subtilis, bacillus licheniformis, bacillus coagulans, a protein, an amino acid and clostridium butyricum in combination.
Preferably, the preparation method of the fermentation substrate comprises the following steps: processing calcium carbonate by ruby laser, wherein the output power is 2.2-2.8mw, the irradiation processing time is 4-6min, after the irradiation is finished, sending the calcium carbonate into a grinding machine for grinding, grinding the calcium carbonate through a 20-100 mesh sieve, after the grinding is finished, sending the calcium carbonate into water for ultrasonic reaction, and after the reaction is finished, washing and drying the calcium carbonate to obtain the fermentation substrate.
Preferably, the ultrasonic power of the ultrasonic reaction is 100-200W, and the ultrasonic time is 20-30 min.
Preferably, the homogenizing pressure of the homogenizing treatment is 10-30MPa, the homogenizing rotation speed is 500-1000r/min, and the homogenizing time is 35-45 min.
Preferably, the homogenizing pressure is 10-30MPa, the homogenizing rotation speed is 500-.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the feed fermentation system, the montmorillonite raw material is firstly crushed, and then electron beam irradiation-laser shock wave coupling is adopted, so that the surface structure in the raw material is damaged, the dispersing capacity of the raw material is improved, the raw material fermentation treatment is convenient, meanwhile, the added modification liquid is compounded by the raw materials such as tin sulfate and lanthanum chloride, the dispersing effect of the modification liquid can be improved by the added sodium citrate, the tin sulfate and the lanthanum chloride can permeate into the montmorillonite raw material, and then the ionization effect is improved in ionization, so that the structure of the montmorillonite raw material is further damaged, the dispersing capacity and the stability of the montmorillonite raw material are further improved, the feed fermentation system is conveniently combined with a microecological preparation, and the subsequent fermentation effect is improved.
(2) Impurities in the modification solution are removed by washing and centrifuging, the obtained montmorillonite base material is convenient for subsequent fermentation treatment, and after the montmorillonite base material is mixed with a microecological preparation, the microecological preparation is fully dispersed into the montmorillonite base material, so that the fermentation efficiency of the montmorillonite base material and the microecological preparation is improved.
(3) The fermentation substrate is irradiated by calcium carbonate laser, and then the activity and the dispersing capacity of the fermentation substrate are improved in a dispersing mode, so that the fermentation substrate is used as an intermediate material in fermentation, and the microecological preparation and the montmorillonite substrate are polymerized, so that the fermentation efficiency of the microecological preparation and the montmorillonite substrate is further improved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the microbial fermentation feed fermentation system comprises the following operation steps:
the method comprises the following steps: crushing montmorillonite raw material, sieving with 100 mesh sieve, feeding into an electron beam irradiation box for electron beam irradiation-laser shock wave coupling treatment, and taking out for later use;
step two: then sending the mixture into a pretreatment tank, adding the modified solution, stirring and mixing the mixture, wherein the stirring speed is 100r/min, and the stirring time is 30min to obtain a pre-primary material;
step three: feeding the pre-primary material into an ionization tank for ionization treatment, and then washing, centrifuging and drying after ionization to obtain a montmorillonite base material;
step four: mixing montmorillonite base material, molasses, microecological preparation and fermentation base material according to the weight ratio of 6:2:1:1, stirring at the rotation speed of 50r/in for 10min to obtain fermentation raw material;
step five: homogenizing the fermentation material before feeding into a fermentation tank, then performing fermentation treatment at 25 deg.C for 5 hr, and finishing fermentation to obtain fermented feed.
The irradiation dose of the electron beam in the electron beam irradiation-laser shock wave coupling treatment of this example was 1 MeV.
The pulse width of the laser shock wave treatment of the embodiment is 20-30ns, and the laser shock power density is 0.1GW/cm2The energy was 1J and the treatment time was 1 min.
The preparation method of the modified solution in this example is as follows: adding 2 parts of sodium chloride into 50 parts of water, then adding 5 parts of sodium citrate, stirring at the rotating speed of 300r/min for 20min, then adding 1 part of tin sulfate and 1 part of lanthanum chloride, then stirring at the temperature of 70 ℃ for 30min, wherein the stirring rotating speed is 500r/min, and obtaining the modified solution after stirring.
The microecological agent of this example was bacillus subtilis.
The preparation method of the fermentation substrate in this embodiment is as follows: the method comprises the following steps of processing calcium carbonate by ruby laser, wherein the output power is 2.2mw, the irradiation processing time is 4min, after irradiation, sending the calcium carbonate into a grinding machine for grinding, after grinding through a 20-mesh sieve, after grinding, sending the calcium carbonate into water for ultrasonic reaction, after the reaction, washing with water, and drying to obtain the fermentation substrate.
The ultrasonic power of the ultrasonic reaction in this example is 100W, and the ultrasonic time is 20 min.
In the present embodiment, the homogenization pressure is 10MPa, the homogenization rotation speed is 500r/min, and the homogenization time is 35 min.
Example 2:
the microbial fermentation feed fermentation system comprises the following operation steps:
the method comprises the following steps: crushing montmorillonite, sieving with 200 mesh sieve, and then sending into an electron beam irradiation box for electron beam irradiation-laser shock wave coupling treatment, and taking out for later use;
step two: then sending the mixture into a pretreatment tank, adding the modified solution, stirring and mixing the mixture, wherein the stirring speed is 500r/min, and the stirring time is 40min to obtain a pre-primary material;
step three: feeding the pre-primary material into an ionization tank for ionization treatment, and then washing, centrifuging and drying after ionization to obtain a montmorillonite base material;
step four: mixing montmorillonite base material, molasses, microecological preparation and fermentation base material according to the weight ratio of 6:2:1:1, stirring at the rotation speed of 200r/in for 20min to obtain fermentation raw material;
step five: homogenizing the fermentation material before feeding into a fermentation tank, then performing fermentation treatment at 35 deg.C for 10 hr, and finishing fermentation to obtain fermented feed.
The irradiation dose of the electron beam in the electron beam irradiation-laser shock wave coupling treatment of this example was 5 MeV.
The pulse width of the laser shock wave treatment of the embodiment is 20-30ns, and the laser shock power density is 0.3GW/cm2The energy was 3J and the treatment time was 5 min.
The preparation method of the modified solution in this example is as follows: adding 5 parts of sodium chloride into 100 parts of water, then adding 10 parts of sodium citrate, stirring at the rotating speed of 500r/min for 20-30min, then adding 1-4 parts of tin sulfate and 2 parts of lanthanum chloride, then stirring at the temperature of 90 ℃ for 40min, wherein the stirring rotating speed is 1000r/min, and obtaining the modified solution after stirring.
The microecological agent of the present embodiment is one or more combinations of bacillus subtilis, bacillus licheniformis, bacillus coagulans, proteins, amino acids, and clostridium butyricum.
The preparation method of the fermentation substrate in this embodiment is as follows: processing montmorillonite with ruby laser with output power of 2.8mw for 6min, grinding in a grinder after irradiation, grinding through 100 meshes, ultrasonic reacting in water, washing with water, and drying to obtain the fermentation substrate.
The ultrasonic power of the ultrasonic reaction in this example is 200W, and the ultrasonic time is 30 min.
In the present embodiment, the homogenization pressure is 30MPa, the homogenization rotation speed is 1000r/min, and the homogenization time is 45 min.
Example 3:
the microbial fermentation feed fermentation system comprises the following operation steps:
the method comprises the following steps: crushing montmorillonite raw material, sieving with 150 mesh sieve, feeding into an electron beam irradiation box for electron beam irradiation-laser shock wave coupling treatment, and taking out for later use;
step two: then sending the mixture into a pretreatment tank, adding the modified solution, stirring and mixing at the stirring speed of 300r/min for 35min to obtain a pre-primary material;
step three: feeding the pre-primary material into an ionization tank for ionization treatment, and then washing, centrifuging and drying after ionization to obtain a montmorillonite base material;
step four: mixing montmorillonite base material, molasses, microecological preparation and fermentation base material according to the weight ratio of 6:2:1:1, stirring at the rotating speed of 175r/in for 15min to obtain fermentation raw material;
step five: homogenizing the fermentation material before feeding into a fermentation tank, then performing fermentation treatment at 30 deg.C for 15 hr, and finishing fermentation to obtain fermented feed.
The irradiation dose of the electron beam in the electron beam irradiation-laser shock wave coupling treatment of this example was 3 MeV.
Pulse width at the time of laser shock wave processing of the present embodiment20-30ns, and laser impact power density of 0.2GW/cm2The energy was 2J and the treatment time was 3 min.
The preparation method of the modified solution in this example is as follows: adding 3.5 parts of sodium chloride into 75 parts of water, then adding 7.5 parts of sodium citrate, stirring at the rotating speed of 400r/min for 25min, then adding 2.5 parts of tin sulfate and 1.5 parts of lanthanum chloride, and then stirring at the temperature of 80 ℃ for 35min at the rotating speed of 750r/min, and obtaining the modified solution after stirring.
The microecological agent of this example is a combination of bacillus subtilis, bacillus licheniformis, bacillus coagulans, a protein, an amino acid, and clostridium butyricum.
The preparation method of the fermentation substrate in this embodiment is as follows: the method comprises the following steps of processing calcium carbonate by ruby laser, wherein the output power is 2.5mw, the irradiation processing time is 5min, after irradiation, sending the calcium carbonate into a grinding machine for grinding, after grinding through 60 meshes, after grinding, sending the calcium carbonate into water for ultrasonic reaction, after the reaction, washing with water, and drying to obtain the fermentation substrate.
The ultrasonic power of the ultrasonic reaction in this example was 150W, and the ultrasonic time was 25 min.
In the present embodiment, the homogenization pressure is 20MPa, the homogenization rotation speed is 750r/min, and the homogenization time is 40 min.
The homogenizing pressure of the present embodiment is 20MPa, the homogenizing rotation speed is 750r/min, and the homogenizing time is 40 min.
Comparative example 1:
the montmorillonite raw material is not subjected to ionization treatment.
Comparative example 2:
no fermentation substrate was added.
Comparative example 3:
the montmorillonite raw material is not sent into an electron beam irradiation box for treatment.
The pig feeds prepared in example 3 and comparative examples 1-3 of the present invention were fed to 50 piglets of substantially the same size, and the average weight was calculated by weighing the weight of each piglet until 30 days after feeding, and the results are shown in the following table.
Table 1 average body weight test results of the pig feed prepared by the processes of examples and comparative examples when fed to suckling pigs for 30 days are as follows:
as is clear from example 3 and comparative examples 1 to 3, the average body weight of the suckling pig of example 3 of the present invention at 30 days was significantly improved as compared with comparative examples 1 to 3, and it was found that the effect of feed fermentation was significantly improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. A microbial fermentation feed fermentation system is characterized by comprising the following operation steps:
the method comprises the following steps: crushing montmorillonite, crushing the montmorillonite by 100-200 meshes, then sending the crushed montmorillonite into an electron beam irradiation box for electron beam irradiation-laser shock wave coupling treatment, and taking out the montmorillonite for later use after the electron beam irradiation-laser shock wave coupling treatment is finished;
step two: then sending the mixture into a pretreatment tank, adding a modification liquid, stirring and mixing at the stirring speed of 100-500r/min for 30-40min to obtain a pre-primary material;
step three: feeding the pre-primary material into an ionization tank for ionization treatment, and then washing, centrifuging and drying after ionization to obtain a montmorillonite base material;
step four: mixing montmorillonite base material, molasses, microecological preparation and fermentation base material according to the weight ratio of 6:2:1:1, stirring at the rotation speed of 50-200r/in for 10-20min to obtain fermentation base material;
step five: homogenizing the fermentation material before feeding into a fermentation tank, and fermenting at 25-35 deg.C for 5-15 hr to obtain fermented feed.
2. The system for fermenting feed by utilizing microorganisms as claimed in claim 1, wherein the irradiation dose of the electron beam during the electron beam irradiation-laser shock wave coupling treatment is 1-5 MeV.
3. The system of claim 2, wherein the electron beam irradiation dose is 3 MeV.
4. The system of claim 1, wherein the laser shock wave treatment has a pulse width of 20-30ns and a laser shock power density of 0.1-0.3GW/cm2The energy is 1-3J, and the treatment time is 1-5 min.
5. The system for fermenting the microbial fermented feed according to claim 1, wherein the preparation method of the modified liquid comprises the following steps: adding 2-5 parts of sodium chloride into 50-100 parts of water, then adding 5-10 parts of sodium citrate, stirring at the rotation speed of 500r/min for 20-30min at 300-.
6. The system of claim 1, wherein the microbial preparation is one or more of bacillus subtilis, bacillus licheniformis, bacillus coagulans, proteins, amino acids, and clostridium butyricum.
7. The system for fermenting a microbial fermented feed according to claim 1, wherein the preparation method of the fermentation substrate comprises the following steps: processing calcium carbonate by ruby laser, wherein the output power is 2.2-2.8mw, the irradiation processing time is 4-6min, after the irradiation is finished, sending the calcium carbonate into a grinding machine for grinding, grinding the calcium carbonate through a 20-100 mesh sieve, after the grinding is finished, sending the calcium carbonate into water for ultrasonic reaction, and after the reaction is finished, washing and drying the calcium carbonate to obtain the fermentation substrate.
8. The system as claimed in claim 7, wherein the ultrasonic power of the ultrasonic reaction is 100-200W, and the ultrasonic time is 20-30 min.
9. The system as claimed in claim 1, wherein the homogenizing pressure is 10-30MPa, the homogenizing speed is 500-1000r/min, and the homogenizing time is 35-45 min.
10. The system as claimed in claim 9, wherein the homogenizing pressure is 10-30MPa, the homogenizing speed is 500-1000r/min, and the homogenizing time is 35-45 min.
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| CN115636497A (en) * | 2022-10-14 | 2023-01-24 | 淮阴工学院 | Preparation method of persulfate activated irradiation modified bentonite loaded nZVI |
| CN115636495A (en) * | 2022-10-13 | 2023-01-24 | 淮阴工学院 | Preparation method of irradiation modified bentonite loaded vulcanized nano zero-valent iron |
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| CN115636497A (en) * | 2022-10-14 | 2023-01-24 | 淮阴工学院 | Preparation method of persulfate activated irradiation modified bentonite loaded nZVI |
| CN115636497B (en) * | 2022-10-14 | 2023-12-01 | 淮阴工学院 | Preparation method of modified bentonite loaded nZVI by persulfate activation irradiation |
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| CN113907382B (en) | 2023-04-07 |
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