CN113349292B - Preparation method of transtumor gastric lipase - Google Patents
Preparation method of transtumor gastric lipase Download PDFInfo
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- CN113349292B CN113349292B CN202110141412.7A CN202110141412A CN113349292B CN 113349292 B CN113349292 B CN 113349292B CN 202110141412 A CN202110141412 A CN 202110141412A CN 113349292 B CN113349292 B CN 113349292B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
- A23K40/35—Making capsules specially adapted for ruminants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
Abstract
The invention discloses a preparation method of novel trans-tumor gastric lipase, belonging to the technical field of enzyme preparations and comprising the following steps: (1) preparing a coating; (2) preparing a core material; (3) coating treatment; (4) carrying out corona treatment; (5) and (4) carrying out cryogenic treatment. The application provides a preparation method of novel trans-tumor gastric lipase, and the finally prepared trans-tumor gastric lipase is added into the daily ration of a milk cow, so that the dry matter intake and milk yield of the milk cow can be remarkably improved, the bioavailability of the daily ration is improved, and the productivity is improved to a great extent.
Description
Technical Field
The invention belongs to the technical field of enzyme preparations, and particularly relates to a preparation method of a tumor-free gastric lipase.
Background
In order to maintain the requirement of milk production, the dairy cows in negative energy balance inevitably use a large amount of body fat for energy supply, the weight of the dairy cows is reduced due to the decomposition of the body fat, the exertion of the milk production genetic potential of the dairy cows is limited, the reproductive performance of the dairy cows is influenced, and meanwhile, the metabolic function of the dairy cows can be caused to be obstructed, so that the dairy cows can suffer from diseases such as ketosis, fatty liver and the like. To increase energy intake, the energy level of the ration must be increased. The common method is to increase the feeding amount of the concentrated feed, which results in that the concentrated ratio of the concentrated feed to the coarse feed is too high, the feed intake of the coarse feed is seriously reduced, the rumen fermentation type is changed, the acetic acid is reduced, the propionic acid is increased, the pH value of the rumen is rapidly reduced, the activity of cellulolytic enzyme is inhibited, the milk fat rate is reduced, and diseases such as rumen acidosis and the like are easily caused.
The problem of insufficient energy of the feed can be solved by adding fat into the daily ration of the high-yield dairy cow, the requirement of the dairy cow on energy is met, and the method has very important significance for improving the body condition of the high-yield dairy cow in the early lactation period and improving the milk yield. In an animal body, various lipases control the processes of digestion, absorption, fat reconstruction, lipoprotein metabolism and the like, and promote the absorption and utilization of nutrient substances, the addition of the lipases to improve the fat digestibility is an effective way, and when the lipases are initially applied to monogastric animals such as pigs, chickens and the like, the lipases can improve the digestion and production performance of the animals on feed nutrients, and the action mechanism is clear. However, the use of lipases in ruminants has been rarely reported, mainly because the direct addition of lipases, most of which are inactivated by microbial destruction in the rumen, can reach the small intestine and are rarely utilized. Therefore, aiming at the problem that the unprocessed lipase is directly added into the daily ration of the dairy cow to degrade most lipase in the rumen in the prior art, the novel rumen-protected lipase processed by a special process is designed to be applied to the dairy cow, the fat digestion amount can be increased by coating the processed lipase, the digestion and absorption of nutrient substances are promoted, the loss of the nutrient substances is reduced, the feed utilization rate is improved, the nitrogen excretion is reduced, and the animal production performance is improved.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the preparation method of the trans-tumor gastric lipase, and the finally prepared trans-tumor gastric lipase is added into the daily ration of the dairy cow, so that the dry matter intake and milk yield of the dairy cow can be obviously improved, the bioavailability of the daily ration is improved, and the productivity is improved to a great extent.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing the methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, and taking out for later use after the electron beam irradiation treatment is finished;
b. weighing 10-12 parts by weight of the methylcellulose treated in the operation a, 20-24 parts by weight of lac, 18-22 parts by weight of sodium alginate and 35-45 parts by weight of pure water, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization, and taking out a homogenized product for later use;
c. c, placing the homogeneous product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1: 2-3 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1: 12-16, and extruding into 80-120-mesh pellets for later use;
c. placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.3-0.5%, and obtaining core materials for later use;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating body obtained in the step (3) in a corona discharge instrument for corona treatment, and taking out for later use after the corona treatment is finished;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, and taking out the coating body after the subzero treatment for vacuum packaging.
Further, the irradiation dose is controlled to be 3-5 MeV during the electron beam irradiation treatment in the operation a in the step (1), and the irradiation treatment time is 2-3 min.
Further, the working pressure is controlled to be 80-90 MPa during the homogenization treatment in the operation b of the step (1).
Further, the rotation speed of the dispersion cylinder during the dispersion treatment in the operation c of the step (1) is 400 to 500rpm, and the frequency of the ultrasonic wave during the ultrasonic treatment is controlled to be 30 to 50 kHz.
By adopting the technical scheme, the methyl cellulose is placed in an electron beam irradiation box for electron beam irradiation treatment, when methylcellulose is subjected to high energy electron beam radiation, the energy of the incident electron beam radiation is lost, atoms in the impacted molecules are released, the atoms are excited, a certain amount of active free radicals are formed on the molecular chain skeleton, so that the activity of the methyl cellulose is improved, then the treated methylcellulose, lac, sodium alginate and pure water are put into a micro-jet high-pressure homogenizer according to a proper proportion for high-pressure homogenization treatment, the materials are impacted at high speed in the oscillation reaction cavity, the materials are subjected to high-frequency oscillation, instantaneous pressure drop, strong shearing, cavitation action and the like, so that the homogeneous fusion of the raw materials is promoted, finally, the obtained homogeneous product is placed in a dispersion cylinder and is uniformly dispersed under the action of ultrasonic waves, and finally, the prepared coating has certain activity.
Further, the temperature is controlled to be 40-50 ℃ and the vacuum degree is 1.3-1.7 Pa during the low-temperature vacuum drying treatment in the operation c of the step (2).
By adopting the technical scheme, the lipase and the carrier are prepared into the pellets together, so that the subsequent processing is facilitated.
Further, the control voltage during the corona treatment in the step (4) is 10-12 kV.
Further, the time of the deep cooling treatment in the step (5) is 3-5 min.
By adopting the technical scheme, the prepared coating is coated on the surface of the core material to form the coating body, then corona treatment is carried out, a low-temperature plasma region can be formed on the surface of the coating body through discharge, so that the activity of the coating body is improved, the digestion and absorption of a finished product after intake are promoted, cryogenic treatment is carried out, and the stability of the structure and the performance of the coating body is ensured by controlling the time of the cryogenic treatment.
Compared with the prior art, the invention has the following advantages:
the application provides a preparation method of the trans-tumor gastric lipase, the finally prepared trans-tumor gastric lipase is added into the daily ration of the dairy cow, the dry matter intake and the milk yield of the dairy cow can be obviously improved, the bioavailability of the daily ration is improved, and the productivity is improved to a great extent.
Detailed Description
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing the methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 3-5 MeV, controlling the irradiation treatment time to be 2-3 min, and taking out for later use after the irradiation treatment is finished;
b. weighing 10-12 parts by weight of the methylcellulose treated in the operation a, 20-24 parts by weight of lac, 18-22 parts by weight of sodium alginate and 35-45 parts by weight of pure water, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 80-90 MPa, and taking out a homogenized product for later use;
c. b, placing the homogeneous product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 400-500 rpm, and the frequency of the ultrasonic wave is controlled to be 30-50 kHz;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1: 2-3 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1: 12-16, and extruding into 80-120-mesh pellets for later use;
c. placing the pellets obtained in the operation b into a vacuum drying oven, and performing low-temperature vacuum drying until the water content is 0.3-0.5% to obtain core materials for later use, wherein the low control temperature is 40-50 ℃, and the vacuum degree is 1.3-1.7 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 10-12 kV, and taking out for later use after completion;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, taking out after 3-5 min of treatment, and carrying out vacuum packaging.
For further explanation of the present invention, reference will now be made to the following specific examples.
Example 1
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing the methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 3MeV and the irradiation treatment time to be 2min, and taking out for later use after the irradiation treatment is finished;
b. weighing 10 parts of methyl cellulose, 20 parts of lac, 18 parts of sodium alginate and 35 parts of pure water according to the corresponding parts by weight after the treatment in the operation a, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 80MPa, and taking out a homogenized product for later use;
c. b, placing the homogenized product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 400rpm, and the frequency of the ultrasonic wave is controlled to be 30 kHz;
(2) preparing a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:2 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:12:12, and extruding into 80-mesh pellets for later use;
c. c, placing the pellets obtained in the operation b in a vacuum drying oven for low-temperature vacuum drying until the water content is 0.3%, and obtaining core materials for later use, wherein the low control temperature is 40 ℃, and the vacuum degree is 1.3 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 10-12 kV, and taking out for later use after completion;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, taking out after 3min of treatment, and carrying out vacuum packaging.
Example 2
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 4MeV and the irradiation treatment time to be 2.5min, and taking out for later use after the irradiation treatment is finished;
b. weighing 11 parts of methyl cellulose, 22 parts of lac, 20 parts of sodium alginate and 40 parts of pure water according to the corresponding parts by weight after the treatment in the operation a, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 85MPa, and taking out a homogenized product for later use;
c. b, placing the homogenized product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 450rpm, and the frequency of the ultrasonic wave is controlled to be 40 kHz;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:2.5 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:14:14, and extruding into pellets of 100 meshes for later use;
c. c, placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.4%, and obtaining core materials for later use, wherein the low control temperature is 45 ℃ and the vacuum degree is 1.5 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating body obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 11kV, and taking out for later use after the corona treatment is finished;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, taking out after 4min of treatment, and carrying out vacuum packaging.
Example 3
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing the methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 5MeV and the irradiation treatment time to be 3min, and taking out for later use after the irradiation treatment is finished;
b. weighing 12 parts of methyl cellulose, 24 parts of lac, 22 parts of sodium alginate and 45 parts of pure water according to the corresponding parts by weight after the treatment in the operation a, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 90MPa, and taking out a homogenized product for later use;
c. b, placing the homogenized product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 500rpm, and the frequency of the ultrasonic wave is controlled to be 50 kHz;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:3 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:16:16, and extruding into 120-mesh pellets for later use;
c. c, placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.5%, and obtaining core materials for later use, wherein the low control temperature is 50 ℃ and the vacuum degree is 1.7 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating body obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 12kV, and taking out for later use after the corona treatment is finished;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, taking out after 5min of treatment, and carrying out vacuum packaging.
Example 4
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. weighing 11 parts of methyl cellulose, 22 parts of lac, 20 parts of sodium alginate and 40 parts of pure water according to the corresponding parts by weight, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 85MPa, and taking out a homogenized product for later use;
b. b, placing the homogenized product obtained in the operation a into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 450rpm, and the frequency of the ultrasonic wave is controlled to be 40 kHz;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:2.5 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:14:14, and extruding into pellets of 100 meshes for later use;
c. c, placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.4%, and obtaining core materials for later use, wherein the low control temperature is 45 ℃ and the vacuum degree is 1.5 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating body obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 11kV, and taking out for later use after the corona treatment is finished;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, taking out after 4min of treatment, and carrying out vacuum packaging.
Example 5
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 4MeV and the irradiation treatment time to be 2.5min, and taking out for later use after the irradiation treatment is finished;
b. weighing 11 parts of methyl cellulose, 22 parts of lac, 20 parts of sodium alginate and 40 parts of pure water according to the corresponding parts by weight after the treatment in the operation a, sequentially placing the components in a homogenizer for homogenizing treatment at normal pressure, and taking out a homogenized product for later use;
c. b, placing the homogenized product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 450rpm, and the frequency of the ultrasonic wave is controlled to be 40 kHz;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:2.5 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:14:14, and extruding into pellets of 100 meshes for later use;
c. c, placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.4%, and obtaining core materials for later use, wherein the low control temperature is 45 ℃ and the vacuum degree is 1.5 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating body obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 11kV, and taking out for later use after the corona treatment is finished;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, taking out after 4min of treatment, and carrying out vacuum packaging.
Example 6
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 4MeV and the irradiation treatment time to be 2.5min, and taking out for later use after the irradiation treatment is finished;
b. weighing 11 parts of methyl cellulose, 22 parts of lac, 20 parts of sodium alginate and 40 parts of pure water according to the corresponding parts by weight after the treatment of the operation a, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 85MPa, and taking out a homogenized product for later use;
c. c, placing the homogeneous product obtained in the operation b into a dispersion cylinder for dispersion treatment, and controlling the rotation speed of the dispersion cylinder to be 450 rpm;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:2.5 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:14:14, and extruding into pellets of 100 meshes for later use;
c. c, placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.4%, and obtaining core materials for later use, wherein the low control temperature is 45 ℃ and the vacuum degree is 1.5 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating body obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 11kV, and taking out for later use after the corona treatment is finished;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, taking out after 4min of treatment, and carrying out vacuum packaging.
Example 7
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 4MeV and the irradiation treatment time to be 2.5min, and taking out for later use after the irradiation treatment is finished;
b. weighing 11 parts of methyl cellulose, 22 parts of lac, 20 parts of sodium alginate and 40 parts of pure water according to the corresponding parts by weight after the treatment of the operation a, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 85MPa, and taking out a homogenized product for later use;
c. b, placing the homogenized product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 450rpm, and the frequency of the ultrasonic wave is controlled to be 40 kHz;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:2.5 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:14:14, and extruding into pellets of 100 meshes for later use;
c. c, placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.4%, and obtaining core materials for later use, wherein the low control temperature is 45 ℃ and the vacuum degree is 1.5 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) cryogenic treatment:
and (4) placing the coated body treated in the step (3) in liquid nitrogen for subzero treatment, taking out after 4min of treatment, and carrying out vacuum packaging.
Example 8
A preparation method of peroxidase for rumen comprises the following steps:
(1) preparing a coating:
a. placing methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, controlling the irradiation dose to be 4MeV and the irradiation treatment time to be 2.5min, and taking out for later use after the irradiation treatment is finished;
b. weighing 11 parts of methyl cellulose, 22 parts of lac, 20 parts of sodium alginate and 40 parts of pure water according to the corresponding parts by weight after the treatment of the operation a, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 85MPa, and taking out a homogenized product for later use;
c. b, placing the homogenized product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment, wherein the rotating speed of the dispersion cylinder is controlled to be 450rpm, and the frequency of the ultrasonic wave is controlled to be 40 kHz;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1:2.5 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1:14:14, and extruding into pellets of 100 meshes for later use;
c. c, placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.4%, and obtaining core materials for later use, wherein the low control temperature is 45 ℃ and the vacuum degree is 1.5 Pa;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
and (4) placing the coating body obtained in the step (3) in a corona discharge instrument for corona treatment, controlling the voltage to be 11kV, and taking out for later use after the corona treatment is finished for vacuum packaging.
In order to compare the technical effects of the present application, the methods of example 2 and examples 4 to 8 were respectively used to correspondingly prepare the rumen bypass ratio of the rumen bypass lipase, and then the rumen bypass ratio performance of the rumen bypass lipase prepared in example 2 and examples 4 to 8 was evaluated:
the results of the rumen degradation rate measurement are shown in Table 1.
Table 1: degradation ratio of Permalus gastric lipase (%), measured by Nylon bag method
As can be seen from the data in the above table 1, the degradation rate of the unprocessed rumen lipase in 12 hours is 95.38%, while the degradation rate of the rumen lipase prepared in example 2 in 12 hours in the rumen is 16.32%, so the rumen bypass rate of the rumen lipase treated by the preparation method is as high as 83%, and the effect is remarkable.
In order to further compare the effect of the application, 70 Holstein cows with 10-15 days after delivery, similar ages and times of gestation and annual milk yield of more than 6 tons are selected from a dairy farm and randomly divided into 7 groups with equal quality and quantity, wherein 10 groups of the 6 groups are test groups, namely, examples 2 and 4-8, and 1 group is a blank control group, and then the method of example 2 and the method of example 4-8 are respectively used for correspondingly preparing the rumen lipase, 10g of the rumen lipase is respectively added into each basic daily ration every day, the basic daily ration is fed, the test cows are fed in a single trough, the cows are fed three times in the morning, in the evening and in the evening, the rumen lipase is extruded for three times, and the rumen lipase is mixed into concentrate twice in the morning and evening and is allowed to be freely taken for 30 days. Feed intake and milk production of cows were recorded 1 day before the test and 10, 20, and 30 days after feeding of the rumen lipase. The specific experimental comparison data are shown in tables 2 and 3 below:
table 2: dry matter intake (kg) of the cows of each group
As can be seen from table 2 above, the application provides a preparation method of the trans-tumor gastric lipase, and the finally prepared trans-tumor gastric lipase is added into the daily ration of the dairy cow, so that the dry matter intake of the dairy cow can be remarkably improved, and the bioavailability of the daily ration is improved.
Table 3: milk yield (kg) of each group of cows
As can be seen from the above table 3, the application provides a preparation method of the trans-tumor gastric lipase, the finally prepared trans-tumor gastric lipase is added into the daily ration of the dairy cow, the milk yield of the dairy cow is obviously improved during the test period, and the productivity is improved to a great extent.
Claims (5)
1. A preparation method of peroxidase in a rumen is characterized by comprising the following steps:
(1) preparing a coating:
a. placing the methyl cellulose in an electron beam irradiation box for electron beam irradiation treatment, and taking out for later use after the electron beam irradiation treatment is finished;
b. weighing 10-12 parts by weight of methyl cellulose, 20-24 parts by weight of shellac, 18-22 parts by weight of sodium alginate and 35-45 parts by weight of pure water which are treated by the operation a, sequentially placing the components in a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, and taking out a homogenized product for later use, wherein the irradiation dose is controlled to be 3-5 MeV during the electron beam irradiation treatment, and the irradiation treatment time is 2-3 min;
c. c, placing the homogeneous product obtained in the operation b into a dispersion cylinder for dispersion treatment, and performing ultrasonic treatment with specific frequency while performing the dispersion treatment;
(2) preparation of a core material:
a. uniformly mixing lipase and hydrogenated oil according to the weight ratio of 1: 2-3 to obtain a mixture A for later use;
b. uniformly mixing the mixture A obtained in the operation a, starch and pure water according to the weight ratio of 1: 12-16, and extruding into 80-120-mesh pellets for later use;
c. placing the pellets obtained in the operation b into a vacuum drying oven for low-temperature vacuum drying until the water content is 0.3-0.5%, and obtaining core materials for later use;
(3) coating treatment:
coating the core material obtained in the step (2) with the coating obtained in the step (1) by adopting a spray drying method, and obtaining a coating body for later use after the coating is finished;
(4) corona treatment:
placing the coating obtained in the step (3) in a corona discharge instrument for corona treatment, and taking out the coating for later use, wherein the control voltage is 10-12 kV during the corona treatment;
(5) cryogenic treatment:
and (4) placing the coating body subjected to corona treatment in the step (4) in liquid nitrogen for subzero treatment, and taking out the coating body after the subzero treatment for vacuum packaging.
2. The method for preparing peroxygastric lipase according to claim 1, wherein the working pressure during the homogenization treatment in the operation b of step (1) is controlled to be 80-90 MPa.
3. The method for preparing peroxidase of rumen according to claim 1, wherein the rotation speed of the dispersion cylinder during the dispersion treatment in operation c of step (1) is 400 to 500rpm, and the frequency of the ultrasonic wave during the ultrasonic treatment is controlled to be 30 to 50 kHz.
4. The method for preparing peroxygastric lipase according to claim 1, wherein the temperature of the low-temperature vacuum drying treatment in the operation c of step (2) is controlled to be 40-50 ℃ and the vacuum degree is 1.3-1.7 Pa.
5. The method for preparing peroxysome gastric lipase according to claim 1, wherein the time of the cryogenic treatment in step (5) is 3-5 min.
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