CN113874495A - 一种生产病毒的方法及收获液组合物 - Google Patents
一种生产病毒的方法及收获液组合物 Download PDFInfo
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Abstract
提供一种生产病毒的方法及收获液组合物。所述方法包括：培养已接种病毒或已转染病毒包装用元件的细胞，使培养得到的细胞与收获液组合物接触，一步收获病毒；所述收获液组合物包含：胰酶、pH缓冲液和任选的核酸酶，pH大于7.5且不超过10.5。
Description
PCT国内申请，说明书已公开。
Claims (24)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2019105748863 | 2019-06-28 | ||
| CN201910574886.3A CN112143693A (zh) | 2019-06-28 | 2019-06-28 | 一种生产病毒的方法及收获液组合物 |
| PCT/CN2020/097901 WO2020259532A1 (zh) | 2019-06-28 | 2020-06-24 | 一种生产病毒的方法及收获液组合物 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113874495A true CN113874495A (zh) | 2021-12-31 |
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| CN201910574886.3A Pending CN112143693A (zh) | 2019-06-28 | 2019-06-28 | 一种生产病毒的方法及收获液组合物 |
| CN202080036017.6A Pending CN113874495A (zh) | 2019-06-28 | 2020-06-24 | 一种生产病毒的方法及收获液组合物 |
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| US (1) | US20220267713A1 (zh) |
| EP (1) | EP3992282A4 (zh) |
| JP (1) | JP2022539511A (zh) |
| CN (2) | CN112143693A (zh) |
| WO (1) | WO2020259532A1 (zh) |
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| CN113373120B (zh) * | 2021-06-18 | 2022-04-26 | 浙江康佰裕生物科技有限公司 | Gmp级逆转录病毒载体的纯化方法与应用 |
| CN115261341B (zh) * | 2022-09-05 | 2024-03-22 | 东曜药业有限公司 | 一种澄清溶瘤痘苗病毒收获液的方法 |
| CN115838694A (zh) * | 2022-10-14 | 2023-03-24 | 杭州明德生物医药技术有限公司 | 一种从细胞收获病毒的方法 |
Citations (4)
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| CN1582333A (zh) * | 2000-09-25 | 2005-02-16 | 波利门科学生物免疫研究有限公司 | 活疫苗及生产方法 |
| WO2012010280A1 (en) * | 2010-07-20 | 2012-01-26 | Bavarian Nordic A/S | Method for harvesting expression products |
| CN103602639A (zh) * | 2013-08-16 | 2014-02-26 | 科兴（大连）疫苗技术有限公司 | 一种采用低渗透压收获液收获病毒的方法 |
| CN103614346A (zh) * | 2013-08-16 | 2014-03-05 | 科兴（大连）疫苗技术有限公司 | 一种采用高渗透压收获液收获病毒的方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPO845497A0 (en) * | 1997-08-08 | 1997-08-28 | Commonwealth Scientific And Industrial Research Organisation | Vaccine for fowlpox virus |
| US6830917B2 (en) * | 2001-12-10 | 2004-12-14 | Baxter Healthcare S.A. | Method of isolation and purification of trypsin from pronase protease and use thereof |
| CN1250284C (zh) * | 2002-10-17 | 2006-04-12 | 中国医学科学院医学生物学研究所 | 甲型肝炎灭活疫苗 |
| US20080138354A1 (en) * | 2006-07-21 | 2008-06-12 | City Of Hope | Cytomegalovirus vaccine |
| EP2240573A4 (en) * | 2008-02-12 | 2011-08-31 | Sanofi Pasteur Ltd | METHOD USING ION EXCHANGE AND GELFILTRATION CHROMATOGRAPHY FOR POXVIRUS CLEANING |
| EP2344634A4 (en) * | 2008-09-24 | 2012-08-29 | Medimmune Llc | METHODS OF CELL CULTURE, AND PROPAGATION AND PURIFICATION OF VIRUSES |
| CN102191225A (zh) * | 2010-03-17 | 2011-09-21 | 李福胜 | 一种用mdck细胞获得流感病毒高产毒株的方法 |
| WO2016201224A1 (en) * | 2015-06-10 | 2016-12-15 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Processes for production and purification of nucleic acid-containing compositions |
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-
2019
- 2019-06-28 CN CN201910574886.3A patent/CN112143693A/zh active Pending
-
2020
- 2020-06-24 JP JP2021576078A patent/JP2022539511A/ja active Pending
- 2020-06-24 WO PCT/CN2020/097901 patent/WO2020259532A1/zh active Application Filing
- 2020-06-24 US US17/596,732 patent/US20220267713A1/en active Pending
- 2020-06-24 CN CN202080036017.6A patent/CN113874495A/zh active Pending
- 2020-06-24 EP EP20832620.7A patent/EP3992282A4/en active Pending
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| CN1582333A (zh) * | 2000-09-25 | 2005-02-16 | 波利门科学生物免疫研究有限公司 | 活疫苗及生产方法 |
| WO2012010280A1 (en) * | 2010-07-20 | 2012-01-26 | Bavarian Nordic A/S | Method for harvesting expression products |
| CN103602639A (zh) * | 2013-08-16 | 2014-02-26 | 科兴（大连）疫苗技术有限公司 | 一种采用低渗透压收获液收获病毒的方法 |
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Also Published As
| Publication number | Publication date |
|---|---|
| US20220267713A1 (en) | 2022-08-25 |
| CN112143693A (zh) | 2020-12-29 |
| WO2020259532A1 (zh) | 2020-12-30 |
| JP2022539511A (ja) | 2022-09-12 |
| TW202115247A (zh) | 2021-04-16 |
| EP3992282A1 (en) | 2022-05-04 |
| EP3992282A4 (en) | 2023-08-16 |
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