CN113855588A - Method for preparing keratin solution by wool hydrolysis - Google Patents
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- CN113855588A CN113855588A CN202111352548.9A CN202111352548A CN113855588A CN 113855588 A CN113855588 A CN 113855588A CN 202111352548 A CN202111352548 A CN 202111352548A CN 113855588 A CN113855588 A CN 113855588A
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- wool
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- enzymolysis
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- 210000002268 wool Anatomy 0.000 title claims abstract description 68
- 102000011782 Keratins Human genes 0.000 title claims abstract description 57
- 108010076876 Keratins Proteins 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000007062 hydrolysis Effects 0.000 title claims description 16
- 238000006460 hydrolysis reaction Methods 0.000 title claims description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000002253 acid Substances 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- 108090000787 Subtilisin Proteins 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 8
- 230000000415 inactivating effect Effects 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 238000005406 washing Methods 0.000 claims abstract description 6
- 239000003513 alkali Substances 0.000 claims abstract description 5
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 5
- 238000004042 decolorization Methods 0.000 claims abstract description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 230000009849 deactivation Effects 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 4
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 3
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 3
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 2
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 235000006408 oxalic acid Nutrition 0.000 claims description 2
- 239000011736 potassium bicarbonate Substances 0.000 claims description 2
- 235000015497 potassium bicarbonate Nutrition 0.000 claims description 2
- 229910000028 potassium bicarbonate Inorganic materials 0.000 claims description 2
- 229910000027 potassium carbonate Inorganic materials 0.000 claims description 2
- 235000011181 potassium carbonates Nutrition 0.000 claims description 2
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 235000017550 sodium carbonate Nutrition 0.000 claims description 2
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims description 2
- 235000010265 sodium sulphite Nutrition 0.000 claims description 2
- 230000020477 pH reduction Effects 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 238000004090 dissolution Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 210000004209 hair Anatomy 0.000 description 8
- 108010009736 Protein Hydrolysates Proteins 0.000 description 7
- 239000008367 deionised water Substances 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- 239000003531 protein hydrolysate Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000002386 leaching Methods 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 108010059378 Endopeptidases Proteins 0.000 description 1
- 102000005593 Endopeptidases Human genes 0.000 description 1
- 102000018389 Exopeptidases Human genes 0.000 description 1
- 108010091443 Exopeptidases Proteins 0.000 description 1
- 206010019049 Hair texture abnormal Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- QKSIFUGZHOUETI-UHFFFAOYSA-N copper;azane Chemical compound N.N.N.N.[Cu+2] QKSIFUGZHOUETI-UHFFFAOYSA-N 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000003700 hair damage Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Dermatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a method for preparing keratin solution by hydrolyzing wool, which comprises the steps of firstly soaking wool in acid solution with certain mass percentage concentration, heating for softening, cooling, washing with water and drying to obtain acidified wool; adding the obtained acidified wool into water, dropwise adding alkali liquor under an ultrasonic condition to adjust the pH value to 8.0-10.5, and filtering; carrying out enzymolysis on the pretreated wool by using subtilisin, inactivating enzyme after the enzymolysis is finished, adding activated carbon for decolorization, and filtering to obtain a hydrolyzed wool keratin dilute solution; and (3) concentrating the dilute hydrolyzed wool keratin solution on a concentrating device to obtain a hydrolyzed keratin solution. The acidification pretreatment in the method reduces the hardness of wool keratin, reduces the difficulty of wool enzymolysis, improves the wool enzymolysis efficiency and reduces the molecular weight of hydrolyzed keratin. The invention has simple process and convenient operation, the wool dissolution rate of the invention can reach more than 70 percent, and the extraction rate can reach more than 60 percent.
Description
Technical Field
The invention belongs to the technical field of keratin preparation methods, and particularly relates to a method for preparing a keratin solution by wool hydrolysis.
Background
Wool contains more than 80% of alpha-keratin, the structure of the alpha-keratin has high similarity with keratin in human hair, and the wool is hydrolyzed to prepare low molecular weight hydrolyzed keratin which can be used as a hair conditioner to be applied to hair washing and protecting products. Because the keratin molecular structure contains a large number of disulfide bonds, the crosslinking is generated inside the peptide chain and among the peptide chains, and a stable three-dimensional network structure is formed, so that the degradation of the keratin macromolecules has certain difficulty. At present, the wool dissolving method mainly comprises a mechanical method, an oxidation method, a reduction method, an acid-base method, a metal salt method, a copper ammonia solution method and the like. Researches show that the methods have certain defects, such as excessive molecular weight, low degradation rate, poor product purity, color, smell and stability and the like, and the obtained products cannot be applied to cosmetics. The enzymolysis method is characterized in that specific biological enzyme is utilized to break specific chemical bonds in protein molecular chains so as to prepare protein hydrolysate, the enzymolysis condition is mild, certain specificity is realized, side reactions are few, and the obtained protein hydrolysate is uniform in composition and is the best way for preparing small-molecule protein hydrolysate. Chinese invention patent CN109689022A describes a low molecular weight keratin hydrolysate, which is prepared by performing oxidation pretreatment of keratin, then performing enzymolysis under the hydrolysis action of endopeptidase and exopeptidase respectively to obtain keratin hydrolysate with molecular weight below 1000Da, and applying the keratin hydrolysate as a conditioner to skin and hair cleaning products.
Disclosure of Invention
The invention provides a method for preparing a keratin solution by hydrolyzing wool in order to overcome the defects in the prior art.
The invention is realized by the following technical scheme: the invention discloses a method for preparing a keratin solution by wool hydrolysis, which comprises the following steps:
(1) soaking wool in an acid solution with a certain mass percentage concentration, heating for softening, cooling, washing with water, and drying to obtain acidified wool;
(2) adding the acidified wool obtained in the step (1) into water, dropwise adding alkali liquor under an ultrasonic condition to adjust the pH value to 8.0-10.5, and filtering;
(3) carrying out enzymolysis on the pretreated wool obtained in the step (2) by using subtilisin, inactivating enzyme after the enzymolysis is finished, adding activated carbon for decolorization, and filtering to obtain a hydrolyzed wool keratin dilute solution;
(4) and (4) filtering and concentrating the dilute hydrolyzed wool keratin solution obtained in the step (3) on a concentrating device to obtain a hydrolyzed keratin solution.
Preferably, the length of the wool in the step (1) is controlled to be less than 5cm, the acid solution is one or more of acetic acid, oxalic acid, citric acid, hydrochloric acid, sulfuric acid or lactic acid, and the mass percentage concentration of the acid solution is 3-15%.
Preferably, in the step (1), wool and the acid solution are mixed according to a mass ratio of 1: feeding materials at a ratio of 5-1: 20, treating for 4-24 hours at a temperature of 60-90 ℃, and drying after cooling and water washing.
Preferably, the alkali liquor in step (2) is one or more of sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, sodium sulfite or sodium bisulfite.
Preferably, the mass percentage of the subtilisin in the acidified wool solution in the step (3) is 0.1-15%; the temperature of the enzymolysis step is 40-75 ℃, the enzymolysis PH = 8.0-10.5, the enzymolysis time is 16-72 h, and the enzyme deactivation condition is heat preservation treatment for 0.5-2 h under the temperature condition of 70-90 ℃; the mass ratio of the active carbon to the enzymolysis solution is (0.1-1): 100, the decoloring temperature is 60-90 ℃, and the decoloring time is 0.5-4 h.
As the best embodiment of the invention, the temperature of the enzymolysis step in the step (3) is 45 ℃, the enzymolysis pH =10, the enzymolysis time is 36h, and the enzyme deactivation condition is heat preservation treatment for 1h under the condition of 80 ℃; the mass ratio of the active carbon to the enzymolysis solution is 1:100, the decoloring temperature is 80 ℃, and the decoloring time is 1.5 h.
The hydrolyzed keratin solution in the step (4) is a clear and transparent yellow solution or a light yellow solution, the mass percentage is more than 18 percent, and the molecular weight is 500Da to 2000 Da.
The invention has the beneficial effects that: the invention aims to provide a method for preparing a keratin solution by wool hydrolysis, and the obtained product can be used as a hair conditioner, enters the interior of hair by means of permeation and surface adsorption and forms a protective film on the surface of the hair so as to improve the problems of hair damage, hair dryness and the like. Generally, the lower the molecular weight of the protein hydrolysate, the better the permeability, but not the film formation; in contrast, protein hydrolysates with a high molecular weight have good film forming properties but poor permeability. In order to fully exert the repairing function of protein hydrolysate from inside to outside, the invention takes wool as raw material, and hydrolyzes the wool according to the processes of acidification pretreatment, primary enzymolysis, decoloration, membrane separation and the like to prepare the low molecular weight keratin solution.
The acidification pretreatment in the method for preparing the keratin solution by hydrolyzing wool reduces the hardness of wool keratin, reduces the difficulty of wool enzymolysis, improves the wool enzymolysis efficiency and reduces the molecular weight of the hydrolyzed keratin. The method for preparing the keratin solution by hydrolyzing the wool has simple process and convenient operation, the wool dissolution rate of the invention can reach more than 70 percent, the extraction rate can reach more than 60 percent, the hydrolysis degree is high, the molecular weight of the obtained keratin is mainly concentrated in 500 Da-2000 Da, the permeability of the keratin in hair is ensured, and better film-forming property is provided.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1:
(1) soaking 20g of wool in 100g of sulfuric acid solution with the mass fraction of 3%, preserving heat at 90 ℃ for 4h, cooling to room temperature, filtering to remove acid solution, leaching with 500g of deionized water, and drying to obtain acidified wool;
(2) putting the wool obtained in the step (1) into 100g of deionized water, and slowly adding Na with the concentration of 0.5% dropwise under the ultrasonic condition2CO3Adjusting the pH value of the solution to be about 10.0, and filtering;
(3) heating to 45 ℃, adjusting the pH =10.0, adding subtilisin with the mass percent of acidified wool being 15% for enzymolysis, keeping the pH at about 10.0 for enzymolysis for 16h, inactivating enzyme after the enzymolysis is finished, heating to 90 ℃ and maintaining for 0.5 h;
(4) adding 1g of activated carbon into the step (3), stirring and decoloring for 0.5h at the temperature of 90 ℃, and filtering after decoloring to obtain a light yellow clear hydrolyzed keratin solution;
(5) purifying and concentrating the hydrolyzed keratin solution obtained in the step (4) by a membrane separation technology to obtain a yellow transparent keratin solution with the mass fraction of more than 18% and the molecular weight of 500 Da-2000 Da.
Example 2:
(1) soaking 10g of wool in 100g of 10% hydrochloric acid solution, preserving heat at 70 ℃ for 12h, cooling to room temperature, filtering to remove the acid solution, leaching with 500g of deionized water, and drying to obtain acidified wool;
(2) putting the wool obtained in the step (1) into 100g of deionized water, slowly dropwise adding NaOH with the concentration of 0.2% under an ultrasonic condition, adjusting the pH value of the solution to be about 9.5, and filtering;
(3) heating to 55 ℃, adjusting the pH =9.5, adding subtilisin with the mass percent of acidified wool being 10% for enzymolysis, keeping the pH at about 9.5 for enzymolysis for 48h, inactivating enzyme after the enzymolysis is finished, heating to 80 ℃ and maintaining for 1 h;
(4) adding 0.7g of activated carbon into the step (3), stirring and decoloring for 2h at the temperature of 80 ℃, and filtering after decoloring to obtain a light yellow clear hydrolyzed keratin solution;
(5) purifying and concentrating the hydrolyzed keratin solution obtained in the step (4) by a membrane separation technology to obtain a yellow transparent keratin solution with the mass fraction of 18% and the molecular weight of 500 Da-2000 Da.
Example 3:
(1) soaking 5g of wool in 100g of acetic acid solution with the mass fraction of 15%, preserving heat at 60 ℃ for 24h, cooling to room temperature, filtering to remove the acid solution, leaching with 500g of deionized water, and drying to obtain acidified wool;
(2) putting the wool obtained in the step (1) into 100g of deionized water, slowly dropwise adding KOH with the concentration of 0.5% under the ultrasonic condition, adjusting the pH of the solution to be about 8, and filtering;
(3) heating to 75 ℃, adjusting the pH =8, adding subtilisin with the mass percent of acidified wool being 1.5% for enzymolysis, keeping the pH at about 8 for enzymolysis for 72h, inactivating enzyme after the enzymolysis is finished, heating to 70 ℃ and maintaining for 2 h;
(4) adding 0.1g of activated carbon into the step (3), stirring and decoloring for 4 hours at the temperature of 60 ℃, and filtering after decoloring to obtain a light yellow clear hydrolyzed keratin solution;
(5) purifying and concentrating the hydrolyzed keratin solution obtained in the step (4) by a membrane separation technology to obtain a yellow transparent keratin solution with the mass fraction of 18% and the molecular weight of 500 Da-2000 Da.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. A method for preparing a keratin solution by wool hydrolysis, which is characterized by comprising the following steps: the method for preparing the keratin solution by hydrolyzing the wool specifically comprises the following steps:
(1) soaking wool in an acid solution with a certain mass percentage concentration, heating for softening, cooling, washing with water, and drying to obtain acidified wool;
(2) adding the acidified wool obtained in the step (1) into water, dropwise adding alkali liquor under an ultrasonic condition to adjust the pH value to 8.0-10.5, and filtering;
(3) carrying out enzymolysis on the pretreated wool obtained in the step (2) by using subtilisin, inactivating enzyme after the enzymolysis is finished, adding activated carbon for decolorization, and filtering to obtain a hydrolyzed wool keratin dilute solution;
(4) and (4) concentrating the dilute hydrolyzed wool keratin solution obtained in the step (3) on a concentrating device to obtain a hydrolyzed keratin solution.
2. A method of wool hydrolysis for the preparation of a keratin solution as claimed in claim 1, characterized in that: the length of the wool in the step (1) is controlled below 5 cm.
3. A method for the preparation of a keratin solution by wool hydrolysis according to claim 1 or 2, characterized in that: the acid solution in the step (1) is one or more of acetic acid, oxalic acid, citric acid, hydrochloric acid, sulfuric acid or lactic acid, and the mass percentage concentration of the acid solution is 3-15%.
4. A method of wool hydrolysis for the preparation of a keratin solution as claimed in claim 3, characterized in that: in the step (1), wool and an acid solution are mixed according to a mass ratio of 1: feeding materials at a ratio of 5-1: 20, treating for 4-24 hours at a temperature of 60-90 ℃, and drying after cooling and water washing.
5. A method of wool hydrolysis for the preparation of a keratin solution as claimed in claim 1, characterized in that: the alkali liquor in the step (2) is one or more of sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, sodium sulfite or sodium bisulfite.
6. A method for the preparation of a keratin solution by wool hydrolysis according to claim 1 or 2, characterized in that: in the step (3), the mass percentage of the subtilisin in the acidified wool solution is 0.1-15%.
7. A method of wool hydrolysis for the preparation of a keratin solution as claimed in claim 6, characterized in that: the temperature of the enzymolysis step in the step (3) is 40-75 ℃, the enzymolysis PH = 8.0-10.5, the enzymolysis time is 16-72 h, and the enzyme deactivation condition is heat preservation treatment for 0.5-2 h at the temperature of 70-90 ℃; the mass ratio of the active carbon to the enzymolysis solution is (0.1-1): 100, the decoloring temperature is 60-90 ℃, and the decoloring time is 0.5-4 h.
8. A method for the preparation of a keratin solution by wool hydrolysis according to claim 1 or 2, characterized in that: the temperature of the enzymolysis step in the step (3) is 50 ℃, the enzymolysis PH =9, the enzymolysis time is 36h, and the enzyme deactivation condition is that the heat preservation treatment is carried out for 0.5h under the condition of 80 ℃; the mass ratio of the active carbon to the enzymolysis solution is 1:100, the decoloring temperature is 80 ℃, and the decoloring time is 1.5 h.
9. A method for the preparation of a keratin solution by wool hydrolysis according to claim 1 or 2, characterized in that: the hydrolyzed keratin solution in the step (4) is a clear and transparent yellow solution or a light yellow solution, the mass percentage is more than 18%, and the molecular weight is 500 Da-2000 Da.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114456253A (en) * | 2022-03-01 | 2022-05-10 | 上海天同纺织科技有限公司 | Waste wool dissolving and keratin extracting process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH037595A (en) * | 1989-06-06 | 1991-01-14 | Seiwa Kasei:Kk | Production of keratin hydrolyzate |
| CN108727485A (en) * | 2018-06-07 | 2018-11-02 | 河南双汇投资发展股份有限公司 | A kind of preparation method of food-grade hydrolysis of keratin |
| CN108794614A (en) * | 2018-06-21 | 2018-11-13 | 澳洋集团有限公司 | The method for extracting wool keratin from waste wool |
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- 2021-11-16 CN CN202111352548.9A patent/CN113855588A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH037595A (en) * | 1989-06-06 | 1991-01-14 | Seiwa Kasei:Kk | Production of keratin hydrolyzate |
| CN108727485A (en) * | 2018-06-07 | 2018-11-02 | 河南双汇投资发展股份有限公司 | A kind of preparation method of food-grade hydrolysis of keratin |
| CN108794614A (en) * | 2018-06-21 | 2018-11-13 | 澳洋集团有限公司 | The method for extracting wool keratin from waste wool |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114456253A (en) * | 2022-03-01 | 2022-05-10 | 上海天同纺织科技有限公司 | Waste wool dissolving and keratin extracting process |
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