CN105602457B - A kind of method that gelatin is prepared by raw material of poultry skin and preparation method thereof - Google Patents
A kind of method that gelatin is prepared by raw material of poultry skin and preparation method thereof Download PDFInfo
- Publication number
- CN105602457B CN105602457B CN201610071118.2A CN201610071118A CN105602457B CN 105602457 B CN105602457 B CN 105602457B CN 201610071118 A CN201610071118 A CN 201610071118A CN 105602457 B CN105602457 B CN 105602457B
- Authority
- CN
- China
- Prior art keywords
- gelatin
- poultry skin
- raw material
- prepared
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108010010803 Gelatin Proteins 0.000 title claims abstract description 80
- 239000008273 gelatin Substances 0.000 title claims abstract description 80
- 229920000159 gelatin Polymers 0.000 title claims abstract description 80
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 80
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 50
- 244000144977 poultry Species 0.000 title claims abstract description 45
- 239000002994 raw material Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 239000002253 acid Substances 0.000 claims abstract description 10
- 239000003513 alkali Substances 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 239000000047 product Substances 0.000 claims description 18
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 230000006837 decompression Effects 0.000 claims description 12
- 230000010355 oscillation Effects 0.000 claims description 12
- 230000033228 biological regulation Effects 0.000 claims description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 9
- XCRBXWCUXJNEFX-UHFFFAOYSA-N peroxybenzoic acid Chemical compound OOC(=O)C1=CC=CC=C1 XCRBXWCUXJNEFX-UHFFFAOYSA-N 0.000 claims description 9
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000002604 ultrasonography Methods 0.000 claims description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- 150000001298 alcohols Chemical class 0.000 claims description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 4
- 150000007529 inorganic bases Chemical class 0.000 claims description 4
- 150000007530 organic bases Chemical class 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 4
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000013459 approach Methods 0.000 claims description 2
- 230000035617 depilation Effects 0.000 claims description 2
- 238000012805 post-processing Methods 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 239000012266 salt solution Substances 0.000 claims description 2
- 238000012423 maintenance Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 9
- 239000000835 fiber Substances 0.000 abstract description 5
- 238000005554 pickling Methods 0.000 abstract description 5
- 238000004042 decolorization Methods 0.000 abstract description 4
- 238000010438 heat treatment Methods 0.000 abstract 1
- 102000008186 Collagen Human genes 0.000 description 7
- 108010035532 Collagen Proteins 0.000 description 7
- 229920001436 collagen Polymers 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000009514 concussion Effects 0.000 description 6
- 241000272525 Anas platyrhynchos Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 210000003746 feather Anatomy 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 241000276707 Tilapia Species 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003518 caustics Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005201 scrubbing Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 206010048282 zoonosis Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09H—PREPARATION OF GLUE OR GELATINE
- C09H1/00—Pretreatment of collagen-containing raw materials for the manufacture of glue
- C09H1/04—Pretreatment of collagen-containing raw materials for the manufacture of glue of hides, hoofs, or leather scrap
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09H—PREPARATION OF GLUE OR GELATINE
- C09H3/00—Isolation of glue or gelatine from raw materials, e.g. by extracting, by heating
- C09H3/02—Purification of solutions of gelatine
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09H—PREPARATION OF GLUE OR GELATINE
- C09H9/00—Drying of glue or gelatine
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Cosmetics (AREA)
- Materials For Medical Uses (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a kind of method that gelatin is prepared by raw material of poultry skin, using discarded useless poultry skin as raw material, a pickling, an alkali cleaning are carried out respectively using ultrasonic acid washing method, ultrasonic pickling is to neutrality again, heating, which is boiled, obtains gelatin solution after certain time, centrifuge and dry obtained product gelatin.Preparation method of the present invention is simple and easy to apply, effectively loose fiber it can simultaneously can be decolourized by carrying out ultrasonic pickling respectively twice, without carrying out decolorization process again, compared with traditional manufacturing of gelatin method, the inventive method is with short production cycle, consume energy less, yield is high, has a good application prospect.
Description
Technical field
The present invention relates to processing of farm products field, more particularly to a kind of method that gelatin is prepared by raw material of poultry skin.
Background technology
Gelatin is the catabolite of collagen, is one of native biopolymer material, is widely used in food, doctor
Edible, pharmagel yield is up to 320,000 tons on the fields such as medicine, daily use chemicals, photography, international markets in 2012.Traditional manufacturing of gelatin master
To be produced by raw material of the osteoderm of the livestocks such as ox, pig, in recent years due to the shadow by zoonosis such as rabid ox disease and aftosas
Sound, many country's limitations or the manufacturing of gelatin for forbidding ox, pig to originate, import are used；On the other hand, due to religious belief, one
A little kosher and Islam area do not receive pig source gelatin product.In recent years, it is bright using aquatic products processing accessory substance as development of raw materials
Glue is as traditional pig, the substitute of ox source gelatin, but the gel characteristic of aquatic products gelatin is poor compared with mammal gelatin, limits significantly
Its application.Therefore, pig, ox source gelatin, water are replaced by development of raw materials high-quality gelatin product of novel mammalian source
Produce gelatin turns into the development trend of gelatin industry.
The collagen tissue that the physicochemical properties of gelatin are not only originated with different animals is closely related, is also produced by gelatin
The influence of technique.It is different by preparation method, gelatin can be divided into alkaline-process gelatin and enzyme process gelatin, acid process gelatin.Alkaline-process gelatin leads to
Be often after material is handled through liming hot water multiple tracks infusion collagen tissue and to obtain gelatin molten, then by the gelatin solution it is concentrated,
Dry, crushing obtains gelatin finished product；This method has the characteristics of gelatin quality is high, but there is liming cycle length (often up to 3-4
Individual month), endure the defects such as glue time length, energy consumption height.Enzyme process gelatin be in the presence of protease hydrolytic collagen tissue and obtain, have
The characteristics of production cycle is shorter, pollution is small；But because enzyme is easily influenceed by pH, Degree of Enzymatic Hydrolysis is difficult to control, this method is suitable only for
Produce the products such as the higher small molecule collagen peptide of palliating degradation degree.Acid technological process be at lower ph infusion collagen tissue and obtain, have
Have the advantages that with short production cycle, equipment is simple, supplies consumption rate is low, product transparency is high.It is disclosed using acid in the prior art
The technology acidification time that method extracts gelatin is generally 3-5 days or so, and its cycle is still longer.
At present, in the market occurs in that more gelatin technology of preparing, and it is bright that such as CN104472849A discloses a kind of edible ox-hide
The preparation method of glue, it includes step:1) by the cattle hide gelatin liquid boiled be diluted to mass concentration be 4-6% cattle hide gelatin it is dilute
Liquid, filtering；2) filtrate is passed through into ion exchange resin；3) it will transmit through liquid and be concentrated into mass concentration through evaporator evaporation for 30-
35% concentrate；4) by step 3) after gained nanofiltration concentrate sterilizing, quick-frozen into ice-like, it is 0.2-1.5mm's to dig into thickness
Roll film；5) drying steps 4 under the conditions of 50-60 DEG C of temperature) gained roll film, moisture is down to mass concentration for 10-12%；6) powder
Broken, mixing, sieving, packaging.A kind of preparation method of tilapia skin gelatin as disclosed in CN103059745A, it is Rofe first
Fish-skin goes the removal of impurity by soda acid immersion, and then fish-skin water swelling controls the acid-base value and Extracting temperature of extraction system,
The extraction for eventually passing certain time obtains gelatin, and the slurry (gelatin not being dried) of acquisition is imported containing liter film
The edible film of roll is obtained in the reel of drying system.The above-mentioned method for preparing gelatin in the prior art all exists bright
The defect that glue decolorizing effect is not good, preparation process is cumbersome, power consumption is high, yield poorly.
Therefore, how to study a kind of preparation method it is simple and easy to apply, can effectively loose fiber and without carry out again decolorization process,
With short production cycle, power consumption less, the high method that gelatin is prepared by raw material of poultry skin of yield be that current technology urgently to be resolved hurrily is asked
Topic.
The content of the invention
A kind of preparation method of present invention offer is simple and easy to apply, effectively simultaneously need not can again carry out decolorization process, life by loose fiber
The production cycle is short, consume energy less, the high method that gelatin is prepared by raw material of poultry skin of yield, can apply to processing of farm products and leads
Domain, has a good application prospect, and can turn waste into wealth.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is as follows：One kind is prepared by raw material of poultry skin
The method of gelatin, is comprised the following steps that：
1) poultry skin is pre-processed：Poultry skin is cleaned up, saline sook, washing after depilation, stripping and slicing；
2) once ultrasound acidifying：The use of acetone is solvent, does not cross the poultry skin pre-processed, addition concentration is 10-30%
Phosphoric acid, regulation pH be 3-4, sonic oscillation frequency be 15.0kHz-30.0kHz；
3) alkali cleaning：Use organic base or inorganic base regulating step 2) obtained solution, it is 10-12 to make its pH；
4) twice ultrasonic is acidified：By step 3) solution decompression concentration after cleaned with water, additions weight be 2-4 times of poultry skin
Alcohols solvent, add concentration be 10-30% hydrochloric acid, regulation pH be 6-7, sonic oscillation frequency be 10.0kHz-
20.0kHz；
5) heat：By step 4) to be rinsed 1-3 time with water after the concentration of obtained solution decompression, addition weight is poultry skin
0.01-0.05 times of benzoyl hydroperoxide and weight is the deionized water of 3-5 times of poultry skin, is heated 2-10 hours at 40-80 DEG C
Obtain gelatin solution；
6) post-process：Product gelatin is obtained after centrifugation, dry, sterilizing.
Further, the step 1) in salt solution be sodium chloride solution that mass fraction is 8-10%, soak time is 2-
5h。
Further, the step 1) in poultry skin stripping and slicing be 3-5mm square fritter.
Further, the step 2) in phosphoric acid concentration be 12%-25%, sonic oscillation frequency be 18.0kHz-
25.0kHz。
Further, the step 3) in organic base can be pyridine, triethylamine.
Further, the step 3) in inorganic base can be NaOH, KOH.
Further, the step 4) in alcohols solvent be ethanol, propyl alcohol or ethylene glycol.
Further, the step 5) in water used be deionized water, benzoyl hydroperoxide consumption is poultry skin weight
0.02-0.04 times.
Further, the step 5) in benzoyl hydroperoxide consumption be 0.025-0.035 times of poultry skin weight.
Further, the step 6) in post-processing approach be specially：By step 5) obtained gelatin solution is in 5000-
Centrifuged 10-20 minutes under the conditions of 7000rpm, take centrifuged supernatant, dried at 30-50 DEG C to moisture and be less than 3%, by temperature
Spend moment be raised to 110-130 DEG C maintain 1-5 seconds after be down to room temperature, obtain product gelatin.
Beneficial effects of the present invention：
Preparation method of the present invention is simple and easy to apply, effectively loose fiber can simultaneously can be taken off by carrying out ultrasonic pickling respectively twice
Color, without carrying out decolorization process again, compared with traditional manufacturing of gelatin method, the inventive method is with short production cycle, consume energy less, yield
Height, has a good application prospect.The present invention is by the ultrasonic effective loose fiber of pickling twice and can improve caustic scrubbing step removing
Grease and foreign protein efficiency are remained, gelatin yield, purity is improved, greatly shortens process time, achieve unexpected
Technique effect, have no in the prior art solve above-mentioned technical problem technical inspiration.
Embodiment
With reference to embodiment, the invention will be further described.Embodiment is intended to carry out citing description to the present invention, and
It is non-to limit the invention in any form.
Embodiment 1：
A kind of method that gelatin is prepared by raw material of poultry skin, is comprised the following steps that：
1) poultry skin is pre-processed：Cock skin is cleaned up, loses hair or feathers, cut the saline sook of mass fraction 8% after 3 × 3mm fritters
2h, washing；
2) once ultrasound acidifying：The use of acetone is solvent, does not cross the cock skin pre-processed, add the phosphorus that concentration is 10%
Acid, regulation pH is 3-4, and sonic oscillation frequency is 20kHz, and the concussion time is 2h；
3) alkali cleaning：Triethylamine is used to add step 2) in obtained solution, it is 10 to make its pH, and soak time is 5h；
4) twice ultrasonic is acidified：By step 3) solution decompression concentration after cleaned with water, add weight be 2 times of cock skin second
Alcohol, adds the hydrochloric acid that concentration is 20%, and regulation pH is 6-7, and sonic oscillation frequency is 15kHz, and the concussion time is 2h；
5) heat：By step 4) rinsed 1 time with water after the concentration of obtained solution decompression, it is 0.01 times of cock skin to add weight
Benzoyl hydroperoxide and the deionized water that weight is 3 times of cock skin, heat 5 hours to obtain gelatin solution at 60 DEG C；
6) post-process：By step 5) obtained gelatin solution centrifuges 10 minutes under the conditions of 5000rpm, takes centrifugation supernatant
Liquid, at 30 DEG C dry to moisture be less than 3%, by temperature moment be raised to 110 DEG C maintain 1 second after be down to room temperature, produced
Product gelatin.
Embodiment 2：
A kind of method that gelatin is prepared by raw material of poultry skin, is comprised the following steps that：
1) poultry skin is pre-processed：Duck skin is cleaned up, loses hair or feathers, cut the saline sook of mass fraction 9% after 4 × 4mm fritters
4h, washing；
2) once ultrasound acidifying：The use of acetone is solvent, does not cross the duck skin pre-processed, add the phosphorus that concentration is 20%
Acid, regulation pH is 3-4, and sonic oscillation frequency is 25kH, and the concussion time is 3h；
3) alkali cleaning：Pyridine is used to add step 2) in obtained solution, it is 11 to make its pH, and soak time is 5h；
4) twice ultrasonic is acidified：By step 3) solution decompression concentration after cleaned with water, add weight be 2 times of duck skin third
Alcohol, adds the hydrochloric acid that concentration is 20%, and regulation pH is 6-7, and sonic oscillation frequency is 15kHz, and the concussion time is 3h；
5) heat：By step 4) rinsed 3 times with water after the concentration of obtained solution decompression, it is 0.05 times of duck skin to add weight
Benzoyl hydroperoxide and the deionized water that weight is 5 times of duck skin, heat 10 hours to obtain gelatin solution at 40 DEG C；
6) post-process：By step 5) obtained gelatin solution centrifuges 15 minutes under the conditions of 6000rpm, takes centrifugation supernatant
Liquid, at 40 DEG C dry to moisture be less than 3%, by temperature moment be raised to 120 DEG C maintain 3 seconds after be down to room temperature, produced
Product gelatin.
Embodiment 3：
A kind of method that gelatin is prepared by raw material of poultry skin, is comprised the following steps that：
1) poultry skin is pre-processed：Cock skin is cleaned up, loses hair or feathers, cut the saline sook of mass fraction 10% after 5 × 5mm fritters
5h, washing；
2) once ultrasound acidifying：The use of acetone is solvent, does not cross the cock skin pre-processed, add the phosphorus that concentration is 30%
Acid, regulation pH is 3-4, and sonic oscillation frequency is 20kHz, and the concussion time is 5h；
3) alkali cleaning：KOH is used to add step 2) in obtained solution, it is 12 to make its pH, and soak time is 5h；
4) twice ultrasonic is acidified：By step 3) solution decompression concentration after cleaned with water, add weight be 2 times of cock skin second
Glycol, adds the hydrochloric acid that concentration is 20%, and regulation pH is 6-7, and sonic oscillation frequency is 15kHz, and the concussion time is 5h；
5) heat：By step 4) rinsed 3 times with water after the concentration of obtained solution decompression, it is 0.05 times of cock skin to add weight
Benzoyl hydroperoxide and the deionized water that weight is 5 times of cock skin, heat 3 hours to obtain gelatin solution at 60 DEG C；
6) post-process：By step 5) obtained gelatin solution centrifuges 20 minutes under the conditions of 7000rpm, takes centrifugation supernatant
Liquid, at 50 DEG C dry to moisture be less than 3%, by temperature moment be raised to 130 DEG C maintain 5 seconds after be down to room temperature, produced
Product gelatin.
Reference examples：
The method for not preparing poultry skin gelatin using twice ultrasonic acid wash：
1) poultry skin is pre-processed：Cock skin is cleaned up, loses hair or feathers, cut the saline sook of mass fraction 10% after 5 × 5mm fritters
5h, washing；
2) once it is acidified：The use of acetone is solvent, does not cross the cock skin pre-processed, add the phosphoric acid that concentration is 30%, adjust
Section pH is 3-4, and acidificatoin time is 5h；
3) alkali cleaning：KOH is used to add step 2) in obtained solution, it is 12 to make its pH, and soak time is 5h；
4) after-souring：By step 3) solution decompression concentration after cleaned with water, add weight be 2 times of cock skin second two
Alcohol, adds the hydrochloric acid that concentration is 20%, and regulation pH is 6-7, and acidificatoin time is 5h；
5) heat：By step 4) rinsed 3 times with water after the concentration of obtained solution decompression, add weight going for 5 times of cock skin
Ionized water, heats 3 hours to obtain gelatin solution at 60 DEG C；
6) post-process：By step 5) obtained gelatin solution centrifuges 20 minutes under the conditions of 7000rpm, takes centrifugation supernatant
Liquid, at 50 DEG C dry to moisture be less than 3%, by temperature moment be raised to 130 DEG C maintain 5 seconds after be down to room temperature, produced
Product gelatin.
Embodiment 4：
The product gelatin fish comparative examples that the embodiment 1-3 method that gelatin is prepared by raw material of poultry skin is prepared
Obtained ProductName see the table below its various aspects of performance that compares：
The result of the test of table 1
As can be seen here, as technical scheme has obtained a kind of high collagen content, gel strength height, transmittance
High, coloury pigskin gelatin, it has a good application prospect.
Above-described embodiment is simply to illustrate that the technical concepts and features of the present invention, the purpose is to be to allow in the art
Those of ordinary skill can understand present disclosure and implement according to this, and it is not intended to limit the scope of the present invention.It is all
It is the equivalent change or modification according to made by the essence of present invention, should all covers within the scope of the present invention.
Claims (10)
1. a kind of method that gelatin is prepared by raw material of poultry skin, it is characterised in that comprise the following steps that：
1) poultry skin is pre-processed：Poultry skin is cleaned up, saline sook, washing after depilation, stripping and slicing；
2) once ultrasound acidifying：The use of acetone is solvent, does not cross the poultry skin pre-processed, add the phosphorus that concentration is 10-30%
Acid, regulation pH is 3-4, and sonic oscillation frequency is 15.0kHz-30.0kHz；
3) alkali cleaning：Use organic base or inorganic base regulating step 2) obtained solution, it is 10-12 to make its pH；
4) twice ultrasonic is acidified：By step 3) solution decompression concentration after cleaned with water, add weight be 2-4 times of poultry skin alcohol
Class solvent, adds the hydrochloric acid that concentration is 10-30%, and regulation pH is 6-7, and sonic oscillation frequency is 10.0kHz-20.0kHz；
5) heat：By step 4) to be rinsed 1-3 time with water after the concentration of obtained solution decompression, addition weight is poultry skin 0.01-
0.05 times of benzoyl hydroperoxide and weight is the deionized water of 3-5 times of poultry skin, and gelatin is heated 2-10 hours to obtain at 40-80 DEG C
Solution；
6) post-process：Product gelatin is obtained after centrifugation, dry, sterilizing.
2. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step 1)
Middle salt solution is the sodium chloride solution that mass fraction is 8-10%, and soak time is 2-5h.
3. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step 1)
Middle poultry skin stripping and slicing is the fritter of 3-5mm square.
4. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step 2)
Middle phosphoric acid concentration is 12%-25%, and sonic oscillation frequency is 18.0kHz-25.0kHz.
5. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step 3)
Middle organic base can be pyridine, triethylamine.
6. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step 3)
Middle inorganic base can be NaOH, KOH.
7. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step 4)
Middle alcohols solvent is ethanol, propyl alcohol or ethylene glycol.
8. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step 5)
In water used be deionized water, benzoyl hydroperoxide consumption is 0.02-0.04 times of poultry skin weight.
9. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 8, it is characterised in that：The step 5)
Middle benzoyl hydroperoxide consumption is 0.025-0.035 times of poultry skin weight.
10. a kind of method that gelatin is prepared by raw material of poultry skin as claimed in claim 1, it is characterised in that：The step
6) post-processing approach is specially in：By step 5) obtained gelatin solution centrifuges 10-20 minutes under the conditions of 5000-7000rpm,
Centrifuged supernatant is taken, is dried at 30-50 DEG C to moisture and is less than 3%, temperature moment is raised to 110-130 DEG C of maintenance 1-5
Room temperature is down to after second, product gelatin is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610071118.2A CN105602457B (en) | 2016-02-01 | 2016-02-01 | A kind of method that gelatin is prepared by raw material of poultry skin and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610071118.2A CN105602457B (en) | 2016-02-01 | 2016-02-01 | A kind of method that gelatin is prepared by raw material of poultry skin and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105602457A CN105602457A (en) | 2016-05-25 |
| CN105602457B true CN105602457B (en) | 2017-09-29 |
Family
ID=55982829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610071118.2A Expired - Fee Related CN105602457B (en) | 2016-02-01 | 2016-02-01 | A kind of method that gelatin is prepared by raw material of poultry skin and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105602457B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113603768B (en) * | 2021-07-14 | 2024-01-23 | 烟台德胜海洋生物科技有限公司 | Preparation method of fish-source collagen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903918A (en) * | 2006-07-31 | 2007-01-31 | 华南理工大学 | Method of extracting collagen and method of using collagen to prepare collagen protein |
| CN105086838A (en) * | 2015-08-14 | 2015-11-25 | 浙江省海洋开发研究院 | Preparation method for tuna skin gelatin |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014209101A2 (en) * | 2013-06-28 | 2014-12-31 | Universiti Putra Malaysia | Method of obtaining gelatin from aquatic animals |
-
2016
- 2016-02-01 CN CN201610071118.2A patent/CN105602457B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903918A (en) * | 2006-07-31 | 2007-01-31 | 华南理工大学 | Method of extracting collagen and method of using collagen to prepare collagen protein |
| CN105086838A (en) * | 2015-08-14 | 2015-11-25 | 浙江省海洋开发研究院 | Preparation method for tuna skin gelatin |
Non-Patent Citations (1)
| Title |
|---|
| 鸡皮明胶的制备及性质研究;谢苗等;《中国食品学报》;20070228;第1-6页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105602457A (en) | 2016-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105542663B (en) | A kind of method that gelatin is prepared as raw material using pigskin and preparation method thereof | |
| CN102020705B (en) | Method for preparing spirulina protein isolate | |
| CN104313098A (en) | Method for preparing swimming bladder collagen powder | |
| CN102181319A (en) | Oil making method based on lower-moisture enzymatic auxiliary cold pressing | |
| CN103849670A (en) | Method of preparing high F-value collagen peptide by hydrolyzing anglerfish fishskins | |
| CN101240313B (en) | Method for preparing scale collagen peptide | |
| CN102363715B (en) | Method for preparing gelatin | |
| CN103361393A (en) | Preparation method of andrias oligopeptide | |
| CN110734948A (en) | extraction device and process for extracting selenium polypeptide from soybeans | |
| CN108203567A (en) | The technique that a kind of acid system prepares pharmagel | |
| CN104450845A (en) | Method for preparing sea cucumber polypeptide from sea cucumber endogenous protease | |
| CN102732592A (en) | Method for preparing freshwater fish bone gelatin by enzyme process | |
| CN106884033B (en) | Nano fibroin powder and preparation method thereof | |
| CN104673868A (en) | Method for preparing wild clam meat protein source antioxidant peptide through ultrasound-assisted enzymolysis method | |
| CN104031172A (en) | Method for extracting pectin from banana peel by ultrasonic synergistic ammonium oxalate method | |
| CN105602457B (en) | A kind of method that gelatin is prepared by raw material of poultry skin and preparation method thereof | |
| CN110128391A (en) | Method for extracting cationic anthocyanin from lonicera edulis | |
| CN104388512A (en) | Method for preparing hydrolyzed collagen | |
| CN109645211A (en) | The extracting method of collagen in a kind of ox-hide | |
| CN104910815B (en) | A kind of method that gelatin is extracted from rabbit | |
| CN106591407A (en) | Fish collagen powder and preparing method thereof | |
| CN110339113A (en) | A kind of peach colloidal solution and its preparation method and application | |
| CN105002246A (en) | Fish polypeptide with uniform molecular weight distribution, and preparation method thereof | |
| CN103992746B (en) | A kind of method utilizing the different gel strength pharmagel of Java tilapia skin preparation | |
| WO2023082523A1 (en) | Method for improving extraction rate of chondroitin sulfate prepared from tilapia skull |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180213 Address after: 510000 Guangzhou, Guangzhou, Guangdong, Tianhe District Tianhe District Sha Tai Nan Road (Jin Enterprise Science Park) 510-A134 room Patentee after: GUANGZHOU JULAN HEALTH INDUSTRY RESEARCH INSTITUTE Co.,Ltd. Address before: 528051 K, room, 2, 08-14, 127 Zhangcha Road, Chancheng District, Guangdong, Foshan Patentee before: FOSHAN JUCHENG BIOCHEMISTRY TECHNOLOGY R&D Co.,Ltd. |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20190529 Address after: 510610 Units 03 and 04, 23rd Floor, Junlin Building, 285 Lindong Road, Tianhe District, Guangzhou City, Guangdong Province Patentee after: GUANGZHOU NIUBINLE NUTRITION TECHNOLOGY Co.,Ltd. Address before: 510000 Room 510-A134, 85 Shatainan Road, Tianhe District, Guangzhou City, Guangdong Province Patentee before: GUANGZHOU JULAN HEALTH INDUSTRY RESEARCH INSTITUTE Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for preparing gelatin by adopting poultry skin as raw material and preparation method thereof Effective date of registration: 20190924 Granted publication date: 20170929 Pledgee: Development Zone sub branch of Bank of Guangzhou Co.,Ltd. Pledgor: GUANGZHOU NIUBINLE NUTRITION TECHNOLOGY Co.,Ltd. Registration number: Y2019440000071 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20201102 Granted publication date: 20170929 Pledgee: Development Zone sub branch of Bank of Guangzhou Co.,Ltd. Pledgor: GUANGZHOU NIUBINLE NUTRITION TECHNOLOGY Co.,Ltd. Registration number: Y2019440000071 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: The invention relates to a method for preparing gelatin from poultry skin and a preparation method thereof Effective date of registration: 20210127 Granted publication date: 20170929 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Tianhe branch Pledgor: GUANGZHOU NIUBINLE NUTRITION TECHNOLOGY Co.,Ltd. Registration number: Y2021440000027 |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170929 |