CN106591407A - Fish collagen powder and preparing method thereof - Google Patents
Fish collagen powder and preparing method thereof Download PDFInfo
- Publication number
- CN106591407A CN106591407A CN201611063110.8A CN201611063110A CN106591407A CN 106591407 A CN106591407 A CN 106591407A CN 201611063110 A CN201611063110 A CN 201611063110A CN 106591407 A CN106591407 A CN 106591407A
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- CN
- China
- Prior art keywords
- fish
- skin
- protein powder
- collagen protein
- spray drying
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
The invention provides a fish collagen powder and a preparing method thereof. The preparing method of the fish collagen powder comprises the steps of using water to clean fish skin, raising the temperature to 40-50 DWG C, soaking and softening the fish kin, and rinsing the fish skin to be clean; conducting enzymolysis on the fish skin through papain at the temperature of 50-55 DEG C, and conducting enzymatic hydrolysis for 4-6 hours to obtain fish skin enzymatic hydrolysate, wherein fish skin protein converts into protein polypeptides and amino acid, and input amount of the papain in the fish skin is 0.5-2%wt; conducting decoloration, filtration and sterilization on the fish skin enzymatic hydrolysate through activated carbon to obtain transparent liquid; conducting spray drying on the transparent liquid, wherein the inlet temperature of the spray drying is 165-185 DEG C and the outlet temperature of the spray drying is 82-92 DEG C; passing a product obtained through the spray drying through a (50-150)-mesh screen to obtain the fish collagen powder. According to the preparing method of the fish collagen powder, enzymolysis efficiency is high, and the obtained fish collagen powder is high in purity and easy to digest.
Description
Technical field
The present invention relates to collagen protein powder technology of preparing, more particularly to a kind of fish collagen protein powder and preparation method thereof.
Background technology
Isin glue collagen（Polypeptide）By biology enzyme orientation shearing skill mainly from Tilapia mossambica, the new fresh hide of cod, fish scale
Art is extracted, and product its main component in addition to wild deep-sea cod collagen is various high-tech reparative factors.
Fish collagen protein powder smooths smooth cut, the effects such as repairing wrinkle and brighten beautiful skin.The generation of wrinkle and fine wrinkles with
The natural loss of collagen is relevant, supplements the skin corium that can directly penetrate to the skin after the pure fish collagen protein powder of East Sea pharmacy, weight
Whole skin fibr tissue mechanism, promotes cell metabolism, delaying cell aging to be unfolded fine wrinkles, recovers skin
Compact and elasticity.
Collagen is beauty health-care food, and production collagen generally using hydrolyzing and digesting two methods, digests skill
Art, compares gelatin hydrolysate（Or Animal Bone）Method safety, its natural chemistry knot will not be destroyed because of strong acid, the effect of highly basic
Structure.Animal proteolytic enzyme is generally adopted in existing zymolysis technique, its enzymolysis efficiency is low.
The content of the invention
It is an object of the present invention to being directed to above-mentioned existing zymolysis technique prepares that fish collagen protein powder enzymolysis efficiency is low to ask
Topic, proposes a kind of preparation method of fish collagen protein powder, and the method enzymolysis efficiency is high, the Isin glue collagen powder purity for preparing
It is high, it is easy to digest.
For achieving the above object, the technical solution used in the present invention is：A kind of preparation method of fish collagen protein powder, including
Following steps：
（1）By fish-skin water（Drinking water）Cleaning, is heated up to 40-50 DEG C of immersion and softens, rinsed clean；
（2）Step（1）50-55 DEG C of enzymolysis of the fish-skin Jing papains for obtaining, enzyme digestion reaction 4-6 hours obtain fish-skin enzymolysis
Liquid, the fish-skin albumen in fish-skin enzymolysis liquid is converted into polypeptide and amino acid, and the input amount of papain is in fish-skin
0.5-2%wt；
（3）By step（2）The fish-skin enzymolysis liquid Jing decolorizing with activated carbon that obtains, filtration, sterilizing obtain transparency liquid；
（4）By step（3）The transparency liquid for obtaining is spray-dried, inlet temperature 165-185 DEG C of spray drying, outlet temperature
82-92℃；
（5）By step（4）The product that spray drying is obtained crosses 50-150 mesh sieves, prepares fish collagen protein powder.
Further, the preparation method of fish collagen protein powder, comprises the following steps：
（1）By fish-skin water（Drinking water）Cleaning, is heated up to 40 DEG C of immersions and softens 4-6h, rinsed clean；
（2）By step（1）50-55 DEG C of enzymolysis of the fish-skin Jing papains for obtaining, enzyme digestion reaction 5 hours obtains fish-skin enzymolysis
Liquid, the fish-skin albumen in fish-skin enzymolysis liquid is converted into polypeptide and amino acid, and the input amount of papain is 1% in fish-skin
wt；
（3）By step（2）The fish-skin enzymolysis liquid Jing decolorizing with activated carbon that obtains, filtration, sterilizing obtain transparency liquid；
（4）By step（3）The transparency liquid for obtaining is spray-dried, inlet temperature 165-185 DEG C of spray drying, outlet temperature
82-92℃；
（5）By step（4）The product that spray drying is obtained crosses 80 mesh sieves, prepares fish collagen protein powder, and 0.5kg packagings are interior
The outer aluminium foil bag of polyethylene plastic bag, obtains finished product.
Further, step（1）Fish-skin band water after rinsing is ground using colloid mill and obtains cladding slurry（Water content is 50-
85%）, solid particle size is 0.1-2.0mm in cladding slurry.
Further, step（1）Vinegar is added in fish-skin after rinsing, and carries out ultrasonication, to remove fish-skin raw meat
Taste, the addition of vinegar is 2-3%wt.
Further, by step（2）The fish-skin enzymolysis liquid for obtaining goes out enzyme, and the enzyme that goes out includes being heated to 80-100 degree Celsius, protects
Warm 1h.
Another object of the present invention additionally provides a kind of fish collagen protein powder, is prepared from using said method.
The preparation method of fish collagen protein powder of the present invention is simple, easy, compared with prior art with advantages below：
（1）The present invention solves recovery, the Utilizing question of fish-skin in fish processing, turns waste into wealth, and adds so as to improve aquatic products
The added value of work leftover bits and pieces, improves the comprehensive utilization ratio of fresh-water fishes.
（2）Using collagen protein powder steady quality, quality better obtained by this technique, its collagen content reaches 93%
More than, Vitro Digestibility reaches more than 90%.
（3）The activated centre of the papain that the present invention is adopted contains cysteine, belongs to thiol protease, and it has enzyme
High, heat endurance living is good, natural safe and sanitary.
（4）Fish-skin band water is ground using colloid mill and obtains cladding slurry, can be while hydrolysis result is ensured, when reducing enzymolysis
Between, reduce Papain enzyme dosage.
Description of the drawings
Fig. 1 is the preparation method step schematic diagram of fish collagen protein powder.
Specific embodiment
With reference to embodiments the present invention is further described：
Embodiment 1
Present embodiment discloses a kind of preparation method of fish collagen protein powder, as shown in figure 1, comprising the following steps：
（1）By fish-skin water（Drinking water）Cleaning, is heated up to 40 DEG C of immersions and softens 5h, rinsed clean；Fish-skin band water adopts colloid
Grind and obtain cladding slurry（Water content is 50-85%）, solid particle size is 0.1-1.0mm in cladding slurry.Fish-skin after rinsing
Middle addition vinegar, and ultrasonication is carried out, to remove fish-skin fishy smell, the addition of vinegar is 2%wt.
（2）By step（1）50-55 DEG C of enzymolysis of the fish-skin Jing papains for obtaining, enzyme digestion reaction 5 hours obtains fish-skin
Enzymolysis liquid, the fish-skin albumen in fish-skin enzymolysis liquid is converted into polypeptide and amino acid, the input amount of papain in fish-skin
For 1%wt；Fish-skin enzymolysis liquid is gone out enzyme, the enzyme that goes out includes being heated to 80-100 degree Celsius, is incubated 1h.
（3）By step（2）The fish-skin enzymolysis liquid Jing decolorizing with activated carbon that obtains, filtration, sterilizing obtain transparency liquid；
（4）By step（3）The transparency liquid for obtaining is spray-dried, inlet temperature 165-185 DEG C of spray drying, outlet temperature
82-92℃；
（5）By step（4）The product that spray drying is obtained crosses 80 mesh sieves, prepares fish collagen protein powder, and 0.5kg packagings are interior
The outer aluminium foil bag of polyethylene plastic bag, obtains finished product.
Using collagen protein powder steady quality, quality better obtained by this technique, its collagen content reach 93% with
On, Vitro Digestibility reaches more than 95%.
Embodiment 2
Present embodiment discloses a kind of preparation method of fish collagen protein powder, comprises the following steps：
（1）By fish-skin water（Drinking water）Cleaning, is heated up to 50 DEG C of immersions and softens 5h, rinsed clean；Fish-skin band water adopts colloid
Grind and obtain cladding slurry（Water content is 50-85%）, solid particle size is 1.0-2.0mm in cladding slurry.Fish-skin after rinsing
Middle addition vinegar, and ultrasonication is carried out, to remove fish-skin fishy smell, the addition of vinegar is 3%wt.
（2）By step（1）50-55 DEG C of enzymolysis of the fish-skin Jing papains for obtaining, enzyme digestion reaction 5 hours obtains fish-skin
Enzymolysis liquid, the fish-skin albumen in fish-skin enzymolysis liquid is converted into polypeptide and amino acid, the input amount of papain in fish-skin
For 0.8%wt；Fish-skin enzymolysis liquid is gone out enzyme, the enzyme that goes out includes being heated to 80-100 degree Celsius, is incubated 1h.
（3）By step（2）The fish-skin enzymolysis liquid Jing decolorizing with activated carbon that obtains, filtration, sterilizing obtain transparency liquid；
（4）By step（3）The transparency liquid for obtaining is spray-dried, inlet temperature 165-185 DEG C of spray drying, outlet temperature
82-92℃；
（5）By step（4）The product that spray drying is obtained crosses 100 mesh sieves, prepares fish collagen protein powder, and 0.5kg packagings are interior
The outer aluminium foil bag of polyethylene plastic bag, obtains finished product.
Using collagen protein powder steady quality, quality better obtained by this technique, its collagen content reach 93% with
On, Vitro Digestibility reaches more than 90%.
Finally it should be noted that：Various embodiments above only to illustrate technical scheme, rather than a limitation；To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to
So the technical scheme described in foregoing embodiments can be modified, either which part or all technical characteristic are entered
Row equivalent；And these modifications or replacement, do not make the essence disengaging various embodiments of the present invention technology of appropriate technical solution
The scope of scheme.
Claims (6)
1. a kind of preparation method of fish collagen protein powder, it is characterised in that comprise the following steps：
（1）Fish-skin is cleaned with water, 40-50 DEG C of immersion is heated up to and is softened, rinsed clean；
（2）Step（1）50-55 DEG C of enzymolysis of the fish-skin Jing papains for obtaining, enzyme digestion reaction 4-6 hours obtain fish-skin enzymolysis
Liquid, the fish-skin albumen in fish-skin enzymolysis liquid is converted into polypeptide and amino acid, and the input amount of papain is in fish-skin
0.5-2%wt；
（3）By step（2）The fish-skin enzymolysis liquid Jing decolorizing with activated carbon that obtains, filtration, sterilizing obtain transparency liquid；
（4）By step（3）The transparency liquid for obtaining is spray-dried, inlet temperature 165-185 DEG C of spray drying, outlet temperature
82-92℃；
（5）By step（4）The product that spray drying is obtained crosses 50-150 mesh sieves, prepares fish collagen protein powder.
2. the preparation method of fish collagen protein powder according to claim 1, it is characterised in that comprise the following steps：
（1）Fish-skin is cleaned with water, 40 DEG C of immersions is heated up to and is softened 4-6h, rinsed clean；
（2）By step（1）50-55 DEG C of enzymolysis of the fish-skin Jing papains for obtaining, enzyme digestion reaction 5 hours obtains fish-skin enzymolysis
Liquid, the fish-skin albumen in fish-skin enzymolysis liquid is converted into polypeptide and amino acid, and the input amount of papain is 1% in fish-skin
wt；
（3）By step（2）The fish-skin enzymolysis liquid Jing decolorizing with activated carbon that obtains, filtration, sterilizing obtain transparency liquid；
（4）By step（3）The transparency liquid for obtaining is spray-dried, inlet temperature 165-185 DEG C of spray drying, outlet temperature
82-92℃；
（5）By step（4）The product that spray drying is obtained crosses 80 mesh sieves, prepares fish collagen protein powder.
3. the preparation method of fish collagen protein powder according to claim 1 or claim 2, it is characterised in that step（1）Fish after rinsing
Belt water is ground using colloid mill and obtains cladding slurry, and solid particle size is 0.1-2.0mm in cladding slurry.
4. the preparation method of fish collagen protein powder according to claim 1 or claim 2, it is characterised in that step（1）Fish after rinsing
Vinegar is added in skin, and carries out ultrasonication, to remove fish-skin fishy smell, the addition of vinegar is 2-3%wt.
5. the preparation method of fish collagen protein powder according to claim 1 or claim 2, it is characterised in that by step（2）The fish for obtaining
Skin enzymolysis liquid goes out enzyme, and the enzyme that goes out includes being heated to 80-100 degree Celsius, is incubated 1h.
6. a kind of fish collagen protein powder, it is characterised in that using the system of fish collagen protein powder described in claim 1-5 any one
Preparation Method is prepared from.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611063110.8A CN106591407A (en) | 2016-11-28 | 2016-11-28 | Fish collagen powder and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611063110.8A CN106591407A (en) | 2016-11-28 | 2016-11-28 | Fish collagen powder and preparing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106591407A true CN106591407A (en) | 2017-04-26 |
Family
ID=58593543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611063110.8A Pending CN106591407A (en) | 2016-11-28 | 2016-11-28 | Fish collagen powder and preparing method thereof |
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| Country | Link |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164442A (en) * | 2017-06-05 | 2017-09-15 | 深圳知本康业有限公司 | A kind of collagen polypeptide and application |
| CN108902438A (en) * | 2018-06-21 | 2018-11-30 | 重庆申高生化制药股份有限公司 | Gadus peptide and its enzymatic extraction method |
| CN115944088A (en) * | 2022-12-20 | 2023-04-11 | 江西盖比欧科技有限公司 | Protein powder for body-building meal replacement and preparation method thereof |
Citations (4)
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| CN102533914A (en) * | 2011-12-27 | 2012-07-04 | 广州合诚实业有限公司 | High-purity fishy smell and foreign odor-free fish collagen protein peptide and preparation method thereof |
| CN104498568A (en) * | 2014-11-24 | 2015-04-08 | 威海市桢昊生物技术有限公司 | Method for preparing fish-skin collagen powder by use of fresh fish skin |
| CN104498569A (en) * | 2014-11-24 | 2015-04-08 | 威海市桢昊生物技术有限公司 | Method for preparing fish-skin collagen powder by use of dry fish skin |
| CN105039483A (en) * | 2015-09-21 | 2015-11-11 | 广东海洋大学 | Method for preparing fishskin fish-scale collagen protein |
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2016
- 2016-11-28 CN CN201611063110.8A patent/CN106591407A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533914A (en) * | 2011-12-27 | 2012-07-04 | 广州合诚实业有限公司 | High-purity fishy smell and foreign odor-free fish collagen protein peptide and preparation method thereof |
| CN104498568A (en) * | 2014-11-24 | 2015-04-08 | 威海市桢昊生物技术有限公司 | Method for preparing fish-skin collagen powder by use of fresh fish skin |
| CN104498569A (en) * | 2014-11-24 | 2015-04-08 | 威海市桢昊生物技术有限公司 | Method for preparing fish-skin collagen powder by use of dry fish skin |
| CN105039483A (en) * | 2015-09-21 | 2015-11-11 | 广东海洋大学 | Method for preparing fishskin fish-scale collagen protein |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164442A (en) * | 2017-06-05 | 2017-09-15 | 深圳知本康业有限公司 | A kind of collagen polypeptide and application |
| CN107164442B (en) * | 2017-06-05 | 2020-10-13 | 深圳知本康业有限公司 | Collagen polypeptide and application thereof |
| CN108902438A (en) * | 2018-06-21 | 2018-11-30 | 重庆申高生化制药股份有限公司 | Gadus peptide and its enzymatic extraction method |
| CN108902438B (en) * | 2018-06-21 | 2021-07-30 | 重庆申高生化制药股份有限公司 | Cod peptide and enzymolysis extraction method thereof |
| CN115944088A (en) * | 2022-12-20 | 2023-04-11 | 江西盖比欧科技有限公司 | Protein powder for body-building meal replacement and preparation method thereof |
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Application publication date: 20170426 |
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