CN106544383A - A kind of method that collagen protein is prepared as raw material with fishbone - Google Patents
A kind of method that collagen protein is prepared as raw material with fishbone Download PDFInfo
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- CN106544383A CN106544383A CN201510590254.8A CN201510590254A CN106544383A CN 106544383 A CN106544383 A CN 106544383A CN 201510590254 A CN201510590254 A CN 201510590254A CN 106544383 A CN106544383 A CN 106544383A
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Abstract
The invention discloses a kind of method for preparing collagen protein as raw material with fishbone, step：Fishbone is cleaned, impurity is removed；The pure water of 8 times of fish scale quality, 100 DEG C of steaming and decocting 30min is added to be cooled to 45-50 DEG C, be put into colloid mill micronizing 30min, gained serosity crosses 60 eye mesh screens, is put into 121 DEG C of high temperature sterilize 30min of fermentation tank, is cooled to 35 DEG C；The bacillus subtilises of fishbone slurry volume 5-15% are added, 30-40 DEG C of heat-preservation fermentation 48-72h, 121 DEG C of inactivation 10min are cooled to 45-50 DEG C；Fishbone serosity after enzymolysis is placed under -40 DEG C of 50Pa environment and freezes drying under reduced pressure, gained powder is collagen protein.The present invention, the fishbone discarded after by the use of enzymatic isolation method using Fish processing prepares collagen protein as raw material, and traditional preparation method is prepared with acid-base method, the process of acid-base pair fish bone material is reduced, it is to avoid effective ingredient is destroyed, obtained in of the invention, fish bone collagen protein antigenicity, anaphylaxis are low, possess good gelation, the dispersibility of height, low viscosity, water absorption, and emulsibility.
Description
Technical field
The present invention relates to a kind of method for preparing collagen protein as raw material with fishbone, belongs to marine organisms processing technique field.
Background technology
Collagen protein is a kind of high molecular weight protein of generally existing in animal body, is primarily present in the connective tissue of animal, accounts for the 25-30% of mammal vivo protein.Livestock and poultry animal tissue is always the main path that people obtain natural collagen protein, but due to the generation of the diseases such as bovine spongiform encephalopathy, foot and mouth disease, bird flu, safety of the people to terrestrial mammalian collagen product is made to generate suspection, therefore at present for the research of collagen sources is progressively turning to marine organisms.Aquatic animal especially in its processing waste such as skin, bone, squama contained by abundant collagen protein there is the unexistent advantage of many domestic animal collagen protein, such as certain gelation, dispersibility of height etc..Therefore the collagen protein that safety and sanitation are extracted from aquatic animal becomes a more and more popular in recent years new problem.
The content of the invention
The technical problem to be solved is to provide a kind of method for preparing collagen protein as raw material with fishbone, is different from the traditional method that collagen protein is extracted from mammal.
The solution of above-mentioned technical problem is to employ following technical measures, a kind of method for preparing collagen protein as raw material with fishbone, is mainly comprised the steps：
（1）Fishbone is cleaned, impurity is removed；
（2）The pure water of 8 times of fishbone quality, 100 DEG C of steaming and decocting 30min is added to be cooled to 45-50 DEG C, be put into colloid mill micronizing 30min, gained serosity crosses 60 eye mesh screens, is put into 121 DEG C of high temperature sterilize 30min of fermentation tank, is cooled to 35 DEG C；
（3）The bacillus subtilises of fishbone slurry volume 5-15% are added, 30-40 DEG C of heat-preservation fermentation 48-72h, 121 DEG C of inactivation 10min are cooled to 45-50 DEG C；
（4）Fishbone serosity after enzymolysis is placed under -40 DEG C of 50Pa environment and freezes drying under reduced pressure, gained powder is collagen protein.
The present invention for taking above-mentioned measure, the fishbone discarded after by the use of enzymatic isolation method using Fish processing prepares collagen protein as raw material, and traditional preparation method is prepared with acid-base method, the process of acid-base pair fish bone material is reduced, it is to avoid effective ingredient is destroyed, obtained in of the invention, fish bone collagen protein antigenicity, anaphylaxis are low, possess good gelation, the dispersibility of height, low viscosity, water absorption, and emulsibility.
Specific embodiment
Embodiment 1
（1）100kg shark bones are cleaned, impurity is removed；
（2）800kg pure water, 100 DEG C of steaming and decocting 30min is added to be cooled to 45 DEG C, be put into colloid mill micronizing 30min, gained serosity crosses 60 eye mesh screens, is put into 121 DEG C of high temperature sterilize 30min of fermentation tank, is cooled to 35 DEG C；
（3）The bacillus subtilises of fishbone slurry volume 5% are added, 30 DEG C of heat-preservation fermentation 48h, 121 DEG C of inactivation 10min are cooled to 45 DEG C；
（4）Fishbone serosity after enzymolysis is placed under -40 DEG C of 50Pa environment and freezes drying under reduced pressure, gained powder is collagen protein.
Embodiment 2
（1）200kg fishbones are cleaned, impurity is removed；
（2）1600kg pure water, 100 DEG C of steaming and decocting 30min is added to be cooled to 50 DEG C, be put into colloid mill micronizing 30min, gained serosity crosses 60 eye mesh screens, is put into 121 DEG C of high temperature sterilize 30min of fermentation tank, is cooled to 35 DEG C；
（3）The bacillus subtilises of fishbone slurry volume 15% are added, 40 DEG C of heat-preservation fermentation 72h, 121 DEG C of inactivation 10min are cooled to 50 DEG C；
（4）Fishbone serosity after enzymolysis is placed under -40 DEG C of 50Pa environment and freezes drying under reduced pressure, gained powder is collagen protein.
Claims (1)
1. a kind of method that collagen protein is prepared as raw material with fishbone, it is characterised in that methods described mainly comprises the steps：
（1）Fishbone is cleaned, impurity is removed；
（2）The pure water of 8 times of fishbone quality, 100 DEG C of steaming and decocting 30min is added to be cooled to 45-50 DEG C, be put into colloid mill micronizing 30min, gained serosity crosses 60 eye mesh screens, is put into 121 DEG C of high temperature sterilize 30min of fermentation tank, is cooled to 35 DEG C；
（3）The bacillus subtilises of fishbone slurry volume 5-15% are added, 30-40 DEG C of heat-preservation fermentation 48-72h, 121 DEG C of inactivation 10min are cooled to 45-50 DEG C；
（4）Fishbone serosity after enzymolysis is placed under -40 DEG C of 50Pa environment and freezes drying under reduced pressure, gained powder is collagen protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510590254.8A CN106544383A (en) | 2015-09-17 | 2015-09-17 | A kind of method that collagen protein is prepared as raw material with fishbone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510590254.8A CN106544383A (en) | 2015-09-17 | 2015-09-17 | A kind of method that collagen protein is prepared as raw material with fishbone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106544383A true CN106544383A (en) | 2017-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510590254.8A Pending CN106544383A (en) | 2015-09-17 | 2015-09-17 | A kind of method that collagen protein is prepared as raw material with fishbone |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913749A (en) * | 2018-08-02 | 2018-11-30 | 艾苛密（上海）健康科技股份有限公司 | Active peptide nanometre collagen and preparation method thereof |
| CN109452412A (en) * | 2018-11-15 | 2019-03-12 | 韶关市华工高新技术产业研究院 | One kind brick tea of calcium containing fish and preparation method thereof |
| CN114317657A (en) * | 2021-12-30 | 2022-04-12 | 祥农(北京)食品科技有限公司 | Fishbone peptide and preparation method and application thereof |
| CN114317657B (en) * | 2021-12-30 | 2024-06-28 | 祥农(北京)食品科技有限公司 | Fishbone peptide and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240313A (en) * | 2008-03-07 | 2008-08-13 | 陈成 | Method for preparing collagen peptide |
| CN102690370A (en) * | 2011-12-20 | 2012-09-26 | 浙江省海洋开发研究院 | Comprehensive utilization technique of marine fish bones |
-
2015
- 2015-09-17 CN CN201510590254.8A patent/CN106544383A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240313A (en) * | 2008-03-07 | 2008-08-13 | 陈成 | Method for preparing collagen peptide |
| CN102690370A (en) * | 2011-12-20 | 2012-09-26 | 浙江省海洋开发研究院 | Comprehensive utilization technique of marine fish bones |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913749A (en) * | 2018-08-02 | 2018-11-30 | 艾苛密（上海）健康科技股份有限公司 | Active peptide nanometre collagen and preparation method thereof |
| CN109452412A (en) * | 2018-11-15 | 2019-03-12 | 韶关市华工高新技术产业研究院 | One kind brick tea of calcium containing fish and preparation method thereof |
| CN114317657A (en) * | 2021-12-30 | 2022-04-12 | 祥农(北京)食品科技有限公司 | Fishbone peptide and preparation method and application thereof |
| CN114317657B (en) * | 2021-12-30 | 2024-06-28 | 祥农(北京)食品科技有限公司 | Fishbone peptide and preparation method and application thereof |
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Application publication date: 20170329 |