CN100369929C - Jellyfish collagen and method for preparing the same - Google Patents
Jellyfish collagen and method for preparing the same Download PDFInfo
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- CN100369929C CN100369929C CNB2006100421881A CN200610042188A CN100369929C CN 100369929 C CN100369929 C CN 100369929C CN B2006100421881 A CNB2006100421881 A CN B2006100421881A CN 200610042188 A CN200610042188 A CN 200610042188A CN 100369929 C CN100369929 C CN 100369929C
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Abstract
The present invention discloses a jelly fish collagen protein which is extracted from the mesoglea of jelly fish. The collagen protein is white fibrous solid or yellowish solid powder, wherein the bulk density of the collagen protein is from 0.110 to 0.645 g/ml. The collagen protein is completely dissolved in an acidic solution, and presents a clear transparent solution. The molecular weight of a single chain of the collagen protein is from 100 thousand to 300 thousand daltons, the sugar content is from 2.5 to 5%, and the amino acid content is 80 to 95% mg/100 mg, wherein aspartic acid accounts for 7.9 to 8.3%, glutamic acid accounts for 10.0 to 12.9%, threonine accounts for 3.5 to 4.0%, valine accounts for 3.5 to 3.9%, isoleucine accounts for 2.2 to 2.5%, lysine accounts for 3.4 to 3.8%, and hydroxylysine accounts for 2.0 to 2.7%. A preparation method of the jelly fish collagen protein comprises: firstly, salted jelly fish or fresh jelly fish is used as raw materials; secondly, the raw materials are pretreated; thirdly, mesoglea is separated; fourthly, the mesoglea is dissociated; fifthly, ultrafiltration, separation and concentration are carried out. The collagen protein with the molecular weight of 100 thousand to 300 thousand daltons is obtained, wherein the trapping rate of the collagen protein is from 95% to 98%. The method can be used for effectively recovering proteinase so that a reaction system can be recycled, and the purity of the obtained collagen protein product is high.
Description
Technical field
The present invention relates to the improvement of marine biochemical industry raw material, specifically is a kind of jellyfish collagen and preparation method thereof.It belongs to the biology,drug and chemical industry technical field.
Background technology
Jellyfish has another name called jellyfish, belongs to coelenterates.Jellyfish is carnivorous, and with planktonic organism, little crustaceans, polychetes even little fish are food.Health is umbrella body semisphere, general 30 to 45 centimetres of umbrella footpath, and big reaches 100 centimetres.Be mesogloea (mesoglea) between epidermal area of jellyfish and the gastral layer, mesogloea is very flourishing, has occupied the almost whole thickness of its health, wherein contains fiber and derives from ectodermic cell on a small quantity.Mesogloea is hard and thick, and visible mesogloea has many O-fibers under the electron microscope.Main component in the mesogloea is a moisture, wherein contains the protein and the polysaccharide of minute quantity, and containing organism is 5%, and exists with the form of collagenic protein, and this material has very strong elasticity and bounding force.Jellyfish is the edible macrozooplankton that China has long enjoyed a good reputation, and in recent years, cultures that the boundary has captured artificial breeding, the Xi shape body young survives the winter and the children is bitten technical barrier such as release, and has lasting jellyfish resource provisioning.The Umbrella Rhopilema esculenta main component is protein (collagen protein), does not contain cholesterol and lipid acid.United States Patent (USP) (USpatent6 is arranged, 894,029) reported that utilizing mouthful mouth of hat jellyfish section to be preced with a jellyfish (Stomolophus meleagris) adopts freeze-drying, alkali cleaning, pickling, protease digestion, salt fractionation technology, made class II collagen, be used for the treatment of rheumatoid arthritis.Also have Japanese Patent (JP2004099513) to report the aurelia (Aureiiaaurita) that utilizes ocean palpus jellyfish section, adopt damping fluid extraction collagen, collagen precipitation and separate, collagen dehydration, freeze drying process prepare collagen, the active jellyfish collagen that extracts from aurelia has anti-microbial activity, microorganismPropionibacterium acnes Propionibacterium is the pathogenic bacterium that cause whelk.Domestic more bibliographical information from beef tendon, pigskin, pig cartilage, fish-skin, fish scale, extract the technology and the technology of collagen protein, all be to adopt the enzymolysis extraction process of saltouing, adopting saltouts, and to extract the problem that exists be that proteolytic enzyme reclaims the recycle difficulty that difficulty, salt reclaim difficulty and acidic solution.The jellyfish collagen extracting method of prior art all is to adopt ectoderm in the jellyfish and mesogloea mixed extraction.This inside and outside protoblast foreign protein dissolves and dissociates, and jellyfish collagen is extracted bring influence.Adopt Collagenase to dissociate in addition mostly and combine extraction with the technology of saltouing, the technology of saltouing shortcoming is to adopt a large amount of salt, to forming brine waste.
Summary of the invention
The objective of the invention is to, a kind of jellyfish collagen and preparation method thereof is provided, adopt new purifying process, efficient recovery extracting tool enzyme, recycle reaction system, the active collagen purity height that obtains, recovery rate height.
The objective of the invention is to realize, developed a kind of jellyfish collagen by following technical scheme.Described collagen protein is to extract from the mesogloea of jellyfish, and this collagen protein is white fiber shape solid or light yellow solid powder, and bulk density is 0.110-0.645g/ml, and this collagen protein is dissolving fully in acidic solution, is clear solution; The single chain molecule amount of this collagen protein is 10-30 ten thousand dalton, and sugared content is 2.5-5%, and aminoacids content is 80-95%mg/100mg; Wherein, aspartic acid accounts for 7.9-8.3%, and L-glutamic acid accounts for 10.0-12.9%, and Threonine accounts for 3.5-4.0%, and Xie Ansuan accounts for 3.5-3.9%, and Isoleucine accounts for 2.2-2.5%, and Methionin accounts for 3.4-3.8%, and hydroxylysine accounts for 2.0-2.7%.
A kind of preparation method of above-mentioned jellyfish collagen carries out as follows;
(1) raw material of Cai Yonging is large-scale jellyfish, uses salt marsh jellyfish or fresh jellyfish;
(2) pre-treatment of raw material;
To be basic solution washing, the immersion of 0.1-0.5% with concentration through the jellyfish after the salt marsh dehydration; Soak time 0.5-2h, the pH value of solution after the immersion is 6-8, has ammonia to produce during alkali cleaning, bubble is obvious; The last hypodermal layer of Umbrella Rhopilema esculenta is by original White-opalescent, becomes transparently, and peel-away removal is easily removed unpleasant odor, ammonium ion and impurity;
(3) separate mesogloea;
With the salt marsh dehydration jellyfish after basic solution washing, the immersion, remove transparent last hypodermal layer, can obviously see the mesogloea of white, grind this white mesogloea;
(4) mesogloea that dissociates;
Adopt proteolysis from the ground mesogloea, enzyme dosage 0.1%-2%, stirs the time 10-48h that dissociates by dissociation temperature 10-30 ℃;
(5) ultra-filtration and separation concentrates;
With the dissociated mesogloea disassociation of above-mentioned proteolytic enzyme liquid, adopt the ultrafiltration membrance filter device, the controlling diaphragm intake pressure is at 1-2kg/cm
2, or adopt extra electric field 10-70V/cm, hold back and concentrate molecular weight in 10-30 ten thousand daltonian collagen proteins, its rejection 95%98%;
The disassociation liquid that contains proteolytic enzyme that will be left again adopts cellulose acetate HFA-200 film, proteolytic enzyme is dammed separate, and reclaims this proteolytic enzyme recirculation and uses;
(6) separate spissated collagen protein again through lyophilize, promptly adopt the slow freezing mode, the vacuum tightness of sublimation stage is at 10-30Pa, and condenser temperature obtains white fiber shape solid or light yellow solid powder at-50 ± 0.1 ℃.
The large-scale jellyfish raw material that described (1) step adopts comprises: the jellyfish (Rhopilema esculentum Kishinouye) that the jellyfish of the female section of root saliva (Rhizostomalidae) belongs to; The leaf wrist jellyfish (LobonmaSmithi) of the leaf wrist Medusa (Lobonema) of Rhopilema hispidum Vanhoeffen. (Rhopilema hispidum) and leaf wrist jellyfish section (Lobonematidae).
Described (1) step raw material salt marsh jellyfish quality meets national standard, i.e. moisture, and less than 68%, salt, 18-25%, alum, 1.2-2.2%.
The basic solution that adopts in the pre-treatment of described (2) step raw material is a sodium hydroxide solution, sodium carbonate solution, sodium hydrogen carbonate solution.
Dissociate proteolytic enzyme that mesogloea adopted or be aspartic protease or Sumizyme MP of described (4) step.
Described (4) step dissociates after the mesogloea, also can be with spissated wet collagen protein, again through the collagenase depth hydrolysis, the consumption 0.1-2.0% of this collagenase, dissociation temperature 20-40 ℃, time 10-48h dissociates, obtain hydrolyzed peptide, that is, obtain containing the structure collagen polypeptide of the amino-acid residue about 30, this peptide molecule length is the 30-50 nanometer, molecular weight 3000-20000 dalton.
The ultra-filtration membrane that adopts during described (5) step ultra-filtration and separation concentrates, or employing polymeric film Diaflo (XM30), or employing polyether sulphone film, or employing hollow cellulose film, or adopt asymmetric ultrafiltration UF film, or adopt asymmetric ultrafiltration FS film, or adopt asymmetric ultrafiltration HF film, or adopt asymmetric ultrafiltration T film.
The advantage of jellyfish collagen of the present invention and preparation method thereof is: owing in the pre-treatment of (2) step raw material, will be basic solution washing, the immersion of 0.1-0.5% with concentration through the jellyfish after the salt marsh dehydration; Have ammonia to produce during alkali cleaning, bubble is obvious; Alkali cleaning pre-treatment purpose is with hypodermal layer on the Umbrella Rhopilema esculenta (being epidermal area and gastral layer), by original White-opalescent, becomes transparently, softening peels off easily, removes nonferrous layer; Be the Ammonia material etc. of removing impurity such as alum in the salting process and decomposition again, recover the jellyfish protein-active; Make (3) step separate mesogloea, remove transparent last hypodermal layer, obtain the mesogloea of white smoothly, so that grind this white mesogloea.Owing to go on foot the mesogloea that dissociates be, adopt proteolysis, keep the sugared content of collagen protein simultaneously from the ground mesogloea in (4).Adopt sugared content in phenolsulfuric acid method (Zhang Weijie, the 1999) working sample; With fructose is standard, measures in the 490nm place.Owing to adopt the ultrafiltration and concentration purifying process, can efficient recovery proteolytic enzyme, make the reaction system recycle.The collagen protein product that this technology obtains obviously is different from the feature of the collagen protein that ox, pig, fish etc. extract.Adopt isolating dehydration to take off the mesogloea of raw meat, after rubbing, adopt proteolysis from, ultrafiltration technology, lyophilize obtains the purity height, has active collagen protein.If further obtain collagen polypeptide, not dry collagen through the collagenase depth hydrolysis, can be obtained hydrolyzed peptide again, adopt drying process with atomizing to obtain dry powder, be beneficial to storage.No matter warp is from uv-absorbing, conventional amino acid masses mark is measured, and still measures from infrared spectrum, and the protein gel that is extracted is a collagen protein, conform to the jellyfish collagen of bibliographical information, and the collagen protein purity that obtains is also higher.Jellyfish collagen of the present invention calculates with salt marsh jellyfish raw material, and the collagen protein recovery rate is 3-5%.
Embodiment
Embodiment 1)
Get salt marsh jellyfish 120g, at 0.2M sodium hydroxide solution 100ml, soak 2h, the solution after the immersion is pH6.5, has ammonia to produce during alkali cleaning, and bubble is obvious, can remove ammonium ion and impurity.Umbrella Rhopilema esculenta becomes transparent (similar with the lyophilization jellyfish) by White-opalescent, can obviously see mesogloea.Remove and go up hypodermal layer, white mesogloea is cut into slices and is preserved in the rearmounted refrigerator-freezer.
Get above-mentioned mesogloea, grind, add 0.1M acetate, 20ml adds 600mg stomach en-(magnificent, Sina-American Biotec, pepsin 1: 3000) again, at room temperature stirs the 30h that dissociates.
Adopt the ultrafiltration membrance filter device, the controlling diaphragm intake pressure is at 1-2kg/cm
2, and adopt extra electric field 10-70V/cm, hold back and concentrate molecular weight in 10-30 ten thousand daltonian collagen proteins, its rejection 98%; The ultra-filtration membrane that adopts, or adopt polymeric film Diaflo (XM30), or adopt the polyether sulphone film, or adopt the hollow cellulose film, or adopt asymmetric ultrafiltration UF film, or adopt asymmetric ultrafiltration FS film, or adopt asymmetric ultrafiltration HF film, or adopt asymmetric ultrafiltration T film.
Separate spissated collagen protein again through lyophilize, promptly adopt the slow freezing mode, the vacuum tightness of sublimation stage is at 10-30Pa, and condenser temperature obtains the fibrous light solid collagen protein of 3.851g, its recovery rate 3.21% at-50 ± 0.1 ℃.Jellyfish collagen of the present invention calculates with salt marsh jellyfish raw material, the calculation formula of recovery rate: C%=(m
1/ m) * 100%, wherein, C% is a yield, m
1Be collagen protein weight (g) that m is the weight (g) of salt marsh jellyfish.
The bulk density of this collagen protein is 0.110-0.645g/ml; This collagen protein is dissolving fully in acidic solution, is clear solution; The single chain molecule amount of this collagen protein is 10-30 ten thousand dalton, and sugared content is 2.5-5%, and aminoacids content is 80-95%mg/100mg; Wherein, aspartic acid accounts for 7.9-8.3%, and L-glutamic acid accounts for 10.0-12.9%, and Threonine accounts for 3.5-4.0%, and Xie Ansuan accounts for 3.5-3.9%, and Isoleucine accounts for 2.2-2.5%, and Methionin accounts for 3.4-3.8%, and hydroxylysine accounts for 2.0-2.7%.
The sugared content of collagen protein adopts sugared content in phenolsulfuric acid method (Zhang Weijie, the 1999) working sample.With fructose is standard, measures in the 490nm place.With light absorption value (A490) is X-coordinate, and sugared concentration is X-coordinate, and the drawing standard curve gets equation of linear regression: A490=0.004 * C-0.0016, R=0.9999.Take by weighing the 0.114g sample, be settled to 50ml with the dissolving of 0.5M acetate, measuring sugared content is 3.09%.
The disassociation liquid that contains proteolytic enzyme that will be left again uses cellulose acetate film (HFA-200), and stomach en-(molecular weight 35000) is dammed with recycling use.
The concrete processing condition in embodiment (1)-(7) with the results are shown in Table 1:
Comparative examples:
Get 50g salt marsh jellyfish, 0.2M sodium hydroxide, 100ml soaks 2h, gets the 40g mesogloea, fragmentation, 0.5M acetate 250ml, 0.5% proteolytic enzyme 250mg stirs the room temperature incubation, 48h, 15 ℃.Centrifugal, have to be the thing that dissociates in a large number.3.5M saltout, there is foam to produce, 4.5M adularescent solids, 5.5M has a large amount of flockss, dialysis tubing (8000-12000D) dialysis.Freezing, dry 24h obtains the shallow cotton-shaped white solid of 0.761g.Yield 1.52%.
Jellyfish collagen amino acid of the present invention is formed assay:
Take by weighing exsiccant jellyfish collagen 10mg, adding concentration is the hydrochloric acid soln 2mL of 5.7mol/L, places hydrolysis 24h in the 110 degree baking ovens, measures with the HITACHI835 automatic analyzer for amino acids, and aminoacids content is 92.57mg/100mg.Amino acid is formed content results and is seen Table 2.
Table 2 jellyfish collagen amino acid is formed content results
| The amino acid kind | Jellyfish collagen (mg/100mg) | The amino acid kind | Jellyfish collagen (mg/100mg) |
| Aspartic acid Asp | 8.31 | Xie Ansuan Val | 3.92 |
| L-glutamic acid Glu | 12.99 | Methionine(Met) Met | 0.62 |
| Serine Ser | 3.19 | Phenylalanine Phe | 1.21 |
| Histidine His | 0.25 | Isoleucine Iso | 2.46 |
| Glycine Gly | 18.01 | Leucine Leu | 3.66 |
| Threonine Thr | 4.05 | Methionin Lys | 3.40 |
| L-Ala Ala | 7.74 | Hydroxylysine Hydroxylys | 2.41 |
| Arginine Arg | 7.77 | Proline(Pro) Pro | 7.42 |
| Halfcystine Cys | 0 | Oxyproline Hydroxypro | 3.62 |
| Tyrosine Tyr | 0.33 | Tryptophane Try | 0 |
Those of ordinary skill in the art can understand, and in protection scope of the present invention, makes amendment for the foregoing description, and it all is possible adding and replacing, and it does not all exceed protection scope of the present invention.
Claims (6)
1. the preparation method of a jellyfish collagen, it is characterized in that: described preparation method carries out as follows;
(1) raw material of Cai Yonging is large-scale jellyfish, uses salt marsh jellyfish or fresh jellyfish;
(2) pre-treatment of raw material;
To be basic solution washing, the immersion of 0.1-0.5% with concentration through the jellyfish after the salt marsh dehydration; Soak time 0.5-2h, the pH value of solution after the immersion is 6-8, has ammonia to produce during alkali cleaning, bubble is obvious; The last hypodermal layer of Umbrella Rhopilema esculenta is by original White-opalescent, becomes transparently, and peel-away removal is easily removed unpleasant odor, ammonium ion and impurity;
(3) separate mesogloea;
With the salt marsh dehydration jellyfish after basic solution washing, the immersion, remove transparent last hypodermal layer, can obviously see the mesogloea of white, grind this white mesogloea;
(4) mesogloea that dissociates;
Adopt proteolysis from the ground mesogloea, enzyme dosage 0.1%-2%, stirs the time 10-48h that dissociates by dissociation temperature 10-30 ℃;
(5) ultra-filtration and separation concentrates;
With the dissociated mesogloea disassociation of above-mentioned proteolytic enzyme liquid, adopt the ultrafiltration membrance filter device, the controlling diaphragm intake pressure is at 1-2kg/cm
2, or adopt extra electric field 10-70V/cm, hold back and concentrate molecular weight at 10-30 ten thousand daltonian collagen proteins, its rejection 95%-98%;
The disassociation liquid that contains proteolytic enzyme that will be left again adopts cellulose acetate HFA-200 film, proteolytic enzyme is dammed separate, and reclaims this proteolytic enzyme recirculation and uses;
(6) separate spissated collagen protein again through lyophilize, promptly adopt the slow freezing mode, the vacuum tightness of sublimation stage is at 10-30Pa, and condenser temperature obtains white fiber shape solid or light yellow solid powder at-50 ± 0.1 ℃.
2. according to the preparation method of the described jellyfish collagen of claim 1, it is characterized in that: the large-scale jellyfish raw material that described (1) step adopts comprises: the jellyfish (Rhopilemaesculentum Kishinouye) that the jellyfish of the female section of root saliva (Rhizostomalidae) belongs to; The leaf wrist jellyfish (Lobonma Smithi) of the leaf wrist Medusa (Lobonema) of Rhopilema hispidum Vanhoeffen. (Rhopilema hispidum) and leaf wrist jellyfish section (Lobonematidae).
3. according to the preparation method of the described jellyfish collagen of claim 1, it is characterized in that: the basic solution that adopts in the pre-treatment of described (2) step raw material is a sodium hydroxide solution, sodium carbonate solution, sodium hydrogen carbonate solution.
4. according to the preparation method of the described jellyfish collagen of claim 1, it is characterized in that: dissociate proteolytic enzyme that mesogloea adopted or be aspartic protease or Sumizyme MP of described (4) step.
5. according to the preparation method of the described jellyfish collagen of claim 1, it is characterized in that: described (4) go on foot after the mesogloea that dissociates, also can be with spissated wet collagen protein, again through the collagenase depth hydrolysis, the consumption 0.1-2.0% of this collagenase, dissociation temperature 20-40 ℃, the time 10-48h that dissociates obtains hydrolyzed peptide, promptly, obtain containing the structure collagen polypeptide of the amino-acid residue about 30, this peptide molecule length is the 30-50 nanometer, molecular weight 3000-20000 dalton.
6. according to the preparation method of the described jellyfish collagen of claim 1, it is characterized in that: the ultra-filtration membrane that adopts during described (5) step ultra-filtration and separation concentrates, or employing polymeric film Diaflo, or employing polyether sulphone film, or adopt the hollow cellulose film, or adopt asymmetric ultrafiltration UF film, or adopt asymmetric ultrafiltration FS film, or adopt asymmetric ultrafiltration HF film, or adopt asymmetric ultrafiltration T film.
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101289507B (en) * | 2007-04-17 | 2010-12-22 | 史宗洁 | Collagen protein and collagen polypeptides, preparation thereof and applications |
| CN101331951B (en) * | 2007-06-29 | 2013-08-28 | 丹东海沃水产有限公司 | Preparation method of DHA jellyfish egg polypeptides freeze-dry powder |
| CN101157723B (en) * | 2007-09-24 | 2011-11-16 | 上海水产大学 | Shellfish jacket membrane collagen and method for making same |
| WO2010043073A1 (en) * | 2008-10-16 | 2010-04-22 | Shi Zongjie | Collagen polypeptide, preparation method and uses thereof |
| CN103518941A (en) * | 2012-11-29 | 2014-01-22 | 中国科学院海洋研究所 | Method for preparing jellyfish gonad hydrolyzed protein |
| CN105175533A (en) * | 2015-10-21 | 2015-12-23 | 淄博黄河龙生物工程有限公司 | Process for extracting collagen from thin cow skin |
| CN105255981B (en) * | 2015-11-12 | 2019-04-02 | 福州大学 | A kind of preparation method of non denatured jellyfish collagen |
| CN106819355A (en) * | 2016-11-30 | 2017-06-13 | 山东好当家海洋发展股份有限公司 | The preparation method of one seed oyster polypeptide capsule |
| CN107354027A (en) * | 2017-07-13 | 2017-11-17 | 芜湖慧宇商贸有限公司 | A kind of preparation method of the skin repair soap of the albumen containing jellyfish |
| CN109770275A (en) * | 2019-02-22 | 2019-05-21 | 水母娘娘海洋生物科技有限公司 | A kind of aurelia water and its preparation method and application |
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