CN105175533A - Process for extracting collagen from thin cow skin - Google Patents
Process for extracting collagen from thin cow skin Download PDFInfo
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- CN105175533A CN105175533A CN201510688404.9A CN201510688404A CN105175533A CN 105175533 A CN105175533 A CN 105175533A CN 201510688404 A CN201510688404 A CN 201510688404A CN 105175533 A CN105175533 A CN 105175533A
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Abstract
The invention relates to a process for extracting collagen from thin cow skin, belonging to the technical field of collagen extraction. The process comprises liming, deliming, pre-pickling, pickling, acid washing and skin discharge and is characterized in that organic weak acid and salt are adopted to soak the thin cow skin during pickling; and sodium bicarbonate is firstly added for neutralization and then clear water is used for soaking during acid washing. The process has the beneficial effects that not only can interfibrillar substances be dissolved out but also collagen fibers can be ensured not to be damaged, thus increasing the collagen extraction rate of the thin cow skin.
Description
Technical field
The present invention relates to a kind of thin cow leather collagen extraction process, belong to collagen protein extractive technique field.
Background technology
Collagen protein is a kind of extracellular protein, twists into spiral fibrous protein by 3 peptide chains.Collagen protein is the most rich in protein of people's in-vivo content, accounts for more than 30% of whole body gross protein.Collagen protein contains abundant amino acid, and wherein indispensable amino acid accounts for 15.78% of total amino acid content, and protein nutritive value is high.Collagen product has been widely used in food, protective foods, makeup, medicine and other fields, and the market requirement sharply increases, and therefore the extraction process of collagen protein more and more receives publicity.
The extraction process of collagen protein in current animal skin, be generally animal skin first to pickle through grey alkali, after liming terminates, with a large amount of clean water deliming in rotary drum, pickling cleaning and acidifying expansion is carried out again after deliming, remove and further dispersion agent protofibril with reinforcing fibre interstitial, be convenient to follow-up collagen extraction.In acidification technique, ox-hide carries out adding acid for many times, acid discharge in rotary drum, until ox-hide acidifying is thorough, acid used is hydrochloric acid.After acidifying terminates, carry out washing acid, namely repeatedly clean ox-hide with clear water, to reach the pH requirement of production technique.But this technique exists a problem, in uneven thickness due to ox-hide, thin skin to find to wash rotten washing after acid terminates, collegen filament are havoc, and part collegen filament have entered to be washed in acid solution, causes the reduction of thin skin collagen yield.
Summary of the invention
The object of this invention is to provide a kind of thin cow leather collagen extraction process, it can make interfibrillar substance stripping, can ensure that again collegen filament are not destroyed, improve the collagen yield of thin ox-hide.
Described thin cow leather collagen extraction process, comprises liming, deliming, acidifying, washes acid and go out skin, adopts organic monoacid and salt to soak thin ox-hide during acidifying; First add sodium bicarbonate when washing acid to neutralize, then soak with clear water.
Described organic monoacid is the mixture of acetic acid and citric acid, and mixed weight is than being 1.5-2:1, and organic monoacid consumption is the 1-3% of thin ox-hide quality.By experimental results demonstrate, washing in sour process, adopting the mixture of acetic acid and citric acid as acid rinse, and adopt above blending ratio, very little to the damage degree of the collagen protein in thin ox-hide.
Described salt is sodium-chlor, Repone K or sodium acetate, and consumption is the 0.5-5% of thin ox-hide quality.
When adopting organic monoacid and salt to soak thin ox-hide, the volume adding clear water is the twice of thin ox-hide quality, and soak time is 6-12h.
The add-on of described sodium bicarbonate is the 0.3-1.5% of thin ox-hide quality.
Described thin cow leather collagen extraction process, comprises the following steps when washing acid:
(1) acid solution after acidifying is drained, rinse one time and emptying with clear water;
(2) in the thin ox-hide of step (1) gained, sodium bicarbonate and water is added, waste liquid of draining after rotation;
(3) in the thin ox-hide of step (2) gained, add water, after immersion, go out skin.
In step (2), the volume adding clear water is the twice of thin ox-hide quality, rotates 20-30min.
In step (3), the volume adding clear water is four times of thin ox-hide quality, soaks 2-5h, goes out skin again when the pH value of detection solution is 2-3.
The present invention is directed to problems of the prior art, thin ox-hide is separated separately, to acidifying with wash sour technique and improve.Acid used during acidifying, changes strong acid into organic monoacid and controls its consumption, changes rotation ox-hide into immersion ox-hide, decreases the injury of the external force such as rotation to ox-hide, and adds a small amount of salt and avoid superdistension; Add a certain amount of sodium bicarbonate when washing acid to carry out neutralizing to reduce washing sour number of times, and can skin be gone out after soaking with a large amount of clear water.
Compared with prior art, the present invention has following beneficial effect:
The present invention can make interfibrillar substance stripping, can ensure that again collegen filament are not destroyed, improve the collagen yield of thin ox-hide; Add to decrease after sodium bicarbonate neutralizes and wash sour number of times, energy-conserving and environment-protective.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but it does not limit enforcement of the present invention.
Embodiment 1
Described thin cow leather collagen extraction process is as follows:
(1) liming in rotary drum of thin for 3000kg ox-hide is pickled 15 days;
(2) in the thin ox-hide after pickling, add 6 tons of water, rotate in rotary drum and within 30 minutes, carry out draining deliming, continuous deliming 3 times;
(3) in the thin ox-hide after deliming, add 6 tons of water, 40kg acetic acid and 20kg citric acid, then add 60kg sodium-chlor, after mixing, soak 8 hours;
(4) acid solution of soaking in complete thin ox-hide in step (3) is drained, rinse one time, emptying with 3 tons of water;
(5) add 30kg sodium bicarbonate and 6 tons of water in the thin ox-hide rinsed well to step (4), rotate 20 minutes, waste liquid of draining;
(6) drain in step (5) waste liquid thin ox-hide in add clear water volume be four times of thin ox-hide quality, soak 2 hours, detect when water liquid pH reaches 2.8 and go out skin.
Wash collegen filament in acid solution not obvious, illustrate technique of the present invention wash in sour process very light to the damage degree of collagen protein.
Embodiment 2
Described thin cow leather collagen extraction process is as follows:
(1) liming in rotary drum of thin for 3000kg ox-hide is pickled 15 days;
(2) in the thin ox-hide after pickling, add 6 tons of water, rotate in rotary drum and within 30 minutes, carry out draining deliming, continuous deliming 3 times;
(3) in the thin ox-hide after deliming, add 6 tons of water, 18kg acetic acid and 12kg citric acid, then add 15kg sodium-chlor, after mixing, soak 12 hours;
(4) acid solution of soaking in complete thin ox-hide in step (3) is drained, rinse one time, emptying with 3 tons of water;
(5) add 9kg sodium bicarbonate and 6 tons of water in the thin ox-hide rinsed well to step (4), rotate 30 minutes, waste liquid of draining;
(6) drain in step (5) waste liquid thin ox-hide in add clear water volume be four times of thin ox-hide quality, soak 5 hours, detect when water liquid pH reaches 2.0 and go out skin.
Wash collegen filament in acid solution not obvious, illustrate technique of the present invention wash in sour process very light to the damage degree of collagen protein.
Embodiment 3
Described thin cow leather collagen extraction process is as follows:
(1) liming in rotary drum of thin for 3000kg ox-hide is pickled 15 days;
(2) in the thin ox-hide after pickling, add 6 tons of water, rotate in rotary drum and within 30 minutes, carry out draining deliming, continuous deliming 3 times;
(3) in the thin ox-hide after deliming, add 6 tons of water, 58kg acetic acid and 32kg citric acid, then add 150kg sodium-chlor, after mixing, soak 6 hours;
(4) acid solution of soaking in complete thin ox-hide in step (3) is drained, rinse one time, emptying with 3 tons of water;
(5) add 45kg sodium bicarbonate and 6 tons of water in the thin ox-hide rinsed well to step (4), rotate 25 minutes, waste liquid of draining;
(6) drain in step (5) waste liquid thin ox-hide in add clear water volume be four times of thin ox-hide quality, soak 3 hours, detect when water liquid pH reaches 3.0 and go out skin.
Wash collegen filament in acid solution not obvious, illustrate technique of the present invention wash in sour process very light to the damage degree of collagen protein.
Embodiment 4
Described thin cow leather collagen extraction process is as follows:
(1) liming in rotary drum of thin for 3000kg ox-hide is pickled 15 days;
(2) in the thin ox-hide after pickling, add 6 tons of water, rotate in rotary drum and within 30 minutes, carry out draining deliming, continuous deliming 3 times;
(3) in the thin ox-hide after deliming, add 6 tons of water, 45kg acetic acid and 20kg citric acid, then add 65kg sodium-chlor, after mixing, soak 7 hours;
(4) acid solution of soaking in complete thin ox-hide in step (3) is drained, rinse one time, emptying with 3 tons of water;
(5) add 30kg sodium bicarbonate and 6 tons of water in the thin ox-hide rinsed well to step (4), rotate 25 minutes, waste liquid of draining;
(6) drain in step (5) waste liquid thin ox-hide in add clear water volume be four times of thin ox-hide quality, soak 2.5 hours, detect when water liquid pH reaches 2.7 and go out skin.
Wash collegen filament in acid solution not obvious, illustrate technique of the present invention wash in sour process very light to the damage degree of collagen protein.
Comparative example 1
A kind of thin cow leather collagen extraction process is as follows:
(1) liming in rotary drum of thin for 3000kg ox-hide is pickled 15 days;
(2) in the thin ox-hide after pickling, add 6 tons of water, rotate in rotary drum and within 30 minutes, carry out draining deliming, continuous deliming 3 times;
(3) in the thin ox-hide after deliming, add 6 tons of water and 60kg hydrochloric acid, rotate 20 minutes, draining, continuous pickling 2 times;
(4) acidifying further, adds 6 tons of water and 60kg hydrochloric acid again, rotates 20 minutes, acid discharge, then repeat once in the thin ox-hide in step (3);
(5) in aged thin ox-hide in step (4), add 6 tons of water, rotate 30 minutes, draining, continuous wash 6 times, pH reaches 2.8, goes out skin.
Wash in acid solution and obviously see there are collegen filament, illustrate technique described in comparative example wash in sour process very heavy to the damage degree of collagen protein.
Take out extracting the thin ox-hide after collagen protein in embodiment 1-4 and comparative example, measure the content of oxyproline in skin, and then calculate collagen content, detected result is in table 1.
Detected result in table 1 embodiment 1-4 and comparative example
Claims (8)
1. a thin cow leather collagen extraction process, comprises liming, deliming, acidifying, washes acid and go out skin, it is characterized in that: adopt organic monoacid and salt to soak thin ox-hide during acidifying; First add sodium bicarbonate when washing acid to neutralize, then soak with clear water.
2. thin cow leather collagen extraction process according to claim 1, is characterized in that: organic monoacid is the mixture of acetic acid and citric acid, and mixed weight is than being 1.5-2:1, and organic monoacid consumption is the 1-3% of thin ox-hide quality.
3. thin cow leather collagen extraction process according to claim 1, is characterized in that: salt is sodium-chlor, Repone K or sodium acetate, consumption is the 0.5-5% of thin ox-hide quality.
4. thin cow leather collagen extraction process according to claim 1, is characterized in that: when adopting organic monoacid and salt to soak thin ox-hide, the volume adding clear water is the twice of thin ox-hide quality, and soak time is 6-12h.
5. thin cow leather collagen extraction process according to claim 1, is characterized in that: the add-on of sodium bicarbonate is the 0.3-1.5% of thin ox-hide quality.
6. thin cow leather collagen extraction process according to claim 1, comprises the following steps when it is characterized in that washing acid:
(1) acid solution after acidifying is drained, rinse and emptying with clear water;
(2) in the thin ox-hide of step (1) gained, sodium bicarbonate and water is added, waste liquid of draining after rotation;
(3) in the thin ox-hide of step (2) gained, add water, after immersion, go out skin.
7. thin cow leather collagen extraction process according to claim 6, is characterized in that: in step (2), and the volume adding clear water is the twice of thin ox-hide quality, rotates 20-30min.
8. thin cow leather collagen extraction process according to claim 6, is characterized in that: in step (3), and the volume adding clear water is four times of thin ox-hide quality, soaks 2-5h, goes out skin again when the pH value of detection solution is 2-3.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510688404.9A CN105175533A (en) | 2015-10-21 | 2015-10-21 | Process for extracting collagen from thin cow skin |
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| CN201510688404.9A CN105175533A (en) | 2015-10-21 | 2015-10-21 | Process for extracting collagen from thin cow skin |
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| CN105175533A true CN105175533A (en) | 2015-12-23 |
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| CN201510688404.9A Pending CN105175533A (en) | 2015-10-21 | 2015-10-21 | Process for extracting collagen from thin cow skin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109371086A (en) * | 2018-11-29 | 2019-02-22 | 沾化恒鑫工业科技有限公司 | A method of bovine collagen albumen is produced using cattle hide gelatin tail road glue |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109371086A (en) * | 2018-11-29 | 2019-02-22 | 沾化恒鑫工业科技有限公司 | A method of bovine collagen albumen is produced using cattle hide gelatin tail road glue |
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