CN108794614A - The method for extracting wool keratin from waste wool - Google Patents
The method for extracting wool keratin from waste wool Download PDFInfo
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- CN108794614A CN108794614A CN201810643337.2A CN201810643337A CN108794614A CN 108794614 A CN108794614 A CN 108794614A CN 201810643337 A CN201810643337 A CN 201810643337A CN 108794614 A CN108794614 A CN 108794614A
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- wool
- keratin
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- extracting
- waste wool
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4741—Keratin; Cytokeratin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- Wood Science & Technology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- General Chemical & Material Sciences (AREA)
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- Gastroenterology & Hepatology (AREA)
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- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Processing Of Solid Wastes (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
This application discloses a kind of methods for extracting wool keratin from waste wool, including：(1), discarded wool is pre-processed using hydrogenperoxide steam generator；(2), solution ph is adjusted to 9~11, alkali protease is then added, discarded wool is dissolved by aminosal peptide bond；(3), pass through centrifugation, freezing, dry acquisition keratin solid；(4), it is crushed with pulverizer keratin, obtains wool keratin powder.The method of the present invention can extract wool keratin from waste wool, and the dissolution rate of waste wool is high, and the yield of keratin can reach 91%.
Description
Technical field
This application involves textile technology fields, more particularly to a kind of method for extracting wool keratin from waste wool.
Background technology
Wool keratin matter fiber is as human use's one of natural fiber material earlier, due to wool quality and weaving
The limitation of technology has a large amount of staple fiber, crude fibre to be discarded every year so that the regeneration of this fiber and grinding for method
Study carefully and has always been concerned with.Wool fiber main component is alpha-Keratin, and keratin molecule passes through cystine linkage, hydrogen bond, sat linkage
It acts on forming highly cross-linked three-dimensional stability structure with other keys, due to the complicated compactness of its structure, under general condition
Insoluble, the method for tradition dissolving wool protein is to destroy protein point by reagents such as highly basic, strong acid, reducing agent or oxidants
The cystine linkage or peptide bond of son resolve into small peptide, the environment such as these a large amount of inorganic acids of method generally existing, alkali, reducing agent or oxidants
The waste of unfriendly solvent causes serious influence to environment.
Invention content
The purpose of the present invention is to provide a kind of methods for extracting wool keratin from waste wool, to overcome the prior art
In deficiency.
To achieve the above object, the present invention provides the following technical solutions：
The embodiment of the present application discloses a kind of method for extracting wool keratin from waste wool, including：
(1), discarded wool is pre-processed using hydrogenperoxide steam generator；
(2), solution ph is adjusted to 9~11, and alkali protease is then added by aminosal peptide bond to discarded
Wool is dissolved；
(3), pass through centrifugation, freezing, dry acquisition keratin solid；
(4), it is crushed with pulverizer keratin, obtains wool keratin powder.
Preferably, above-mentioned from the method that waste wool extracts wool keratin, in step (1), at normal temperatures will
Waste wool is added into the hydrogenperoxide steam generator of 20g/L.
Preferably, above-mentioned from the method that waste wool extracts wool keratin, in step (1), pH value of solution is controlled
Value is 10.5~11.
Preferably, above-mentioned from the method that waste wool extracts wool keratin, in step (1), pretreated temperature
Degree is 60~90 DEG C, and processing time is 0.5~2 hour.
Preferably, above-mentioned from the method that waste wool extracts wool keratin, in step (2), alkali protease
Addition be 3.2~3.5%o.w.f.
Preferably, above-mentioned from the method that waste wool extracts wool keratin, in step (2), alkali protease
Enzyme activity be 100~110u/mg.
Preferably, it in step (2), is first heated to from the method that waste wool extracts wool keratin above-mentioned
60 DEG C and 60min is kept, then proceed to be heated to 80 DEG C and keeps 60min, is finally cooled to room temperature.
Compared with the prior art, the advantages of the present invention are as follows：The method of the present invention can extract wool angle from waste wool
The dissolution rate of albumen, waste wool is high, and the yield of keratin can reach 91%.
Specific implementation mode
The present invention is described further by the following example：According to following embodiments, the present invention may be better understood.
However, as it will be easily appreciated by one skilled in the art that specific material ratio, process conditions and its result described in embodiment are only used
In illustrating the present invention, without the present invention described in detail in claims should will not be limited.
Embodiment 1
The method for extracting wool keratin from waste wool：
Waste wool is added into the hydrogenperoxide steam generator of 20g/L at normal temperatures, then control solution ph adds 11
Heat is handled 0.5~2 hour to 60 DEG C.
40~45 DEG C are cooled to, the alkali protease of 3.2%o.w.f is then added in mixed solution, controls pH value of solution
Value is heated to 60 DEG C and keeps 60min, then proceed to be heated to 80 DEG C and keep 60min, then cool to room temperature 10.5.
Pass through centrifugation, freezing, dry acquisition keratin solid.
It is crushed with pulverizer keratin, obtains wool keratin powder.
In the embodiment, the dissolution rate of waste wool has reached 92%, and the yield of keratin reaches 91%.
Embodiment 2
The method for extracting wool keratin from waste wool：
Waste wool is added into the hydrogenperoxide steam generator of 22g/L at normal temperatures, then control solution ph adds 11
Heat is handled 1 hour to 60~90 DEG C.
40 DEG C are cooled to, the alkali protease of 3.5%o.w.f is then added in mixed solution, control solution ph exists
10.5, it is heated to 45 DEG C and keeps 60min, then proceed to be heated to 80 DEG C and keep 60min, then cool to room temperature.
Pass through centrifugation, freezing, dry acquisition keratin solid.
It is crushed with pulverizer keratin, obtains wool keratin powder.
In the embodiment, the dissolution rate of waste wool has reached 90.4%, and the yield of keratin reaches 90.1%.
Embodiment 3
The method for extracting wool keratin from waste wool：
Waste wool is added into the hydrogenperoxide steam generator of 24g/L at normal temperatures, control solution ph is 10.5, then
90 DEG C are heated to, is handled 0.5 hour.
45 DEG C are cooled to, the alkali protease of 3.2%o.w.f is then added in mixed solution, control solution ph exists
10.5, it is heated to 50 DEG C and keeps 45min, then proceed to be heated to 90 DEG C and keep 60min, then cool to room temperature.
Pass through centrifugation, freezing, dry acquisition keratin solid.
It is crushed with pulverizer keratin, obtains wool keratin powder.
In the embodiment, the dissolution rate of waste wool has reached 89.9%, and the yield of keratin reaches 90.3%.
Embodiment 4
The method for extracting wool keratin from waste wool：
Waste wool is added into the hydrogenperoxide steam generator of 20g/L at normal temperatures, control solution ph is 10.5, then
80 DEG C are heated to, is handled 2 hours.
40 DEG C are cooled to, the alkali protease of 3.2%o.w.f is then added in mixed solution, control solution ph exists
9, it is heated to 60 DEG C and keeps 60min, then proceed to be heated to 80 DEG C and keep 50min, then cool to room temperature.
Pass through centrifugation, freezing, dry acquisition keratin solid.
It is crushed with pulverizer keratin, obtains wool keratin powder.
In the embodiment, the dissolution rate of waste wool has reached 90.1%, and the yield of keratin reaches 89.8%.
Embodiment 5
The method for extracting wool keratin from waste wool：
Waste wool is added into the hydrogenperoxide steam generator of 20g/L at normal temperatures, then control solution ph adds 11
Heat is handled 0.5 hour to 90 DEG C.
45 DEG C are cooled to, the alkali protease of 3.5%o.w.f is then added in mixed solution, control solution ph exists
9, it is heated to 50 DEG C and keeps 60min, then proceed to be heated to 90 DEG C and keep 60min, then cool to room temperature.
Pass through centrifugation, freezing, dry acquisition keratin solid.
It is crushed with pulverizer keratin, obtains wool keratin powder.
In the embodiment, the dissolution rate of waste wool has reached 91.7%, and the yield of keratin reaches 90.6%.
Finally, it is to be noted that, the terms "include", "comprise" or its any other variant be intended to it is non-exclusive
Property include so that including a series of elements process, method, article or equipment not only include those elements, but also
Further include other elements that are not explicitly listed, or further include for this process, method, article or equipment it is intrinsic
Element.
Claims (7)
1. a kind of method for extracting wool keratin from waste wool, which is characterized in that including：
(1), discarded wool is pre-processed using hydrogenperoxide steam generator；
(2), solution ph is adjusted to 9~11, and alkali protease is then added by aminosal peptide bond to discarded wool
It is dissolved；
(3), pass through centrifugation, freezing, dry acquisition keratin solid；
(4), it is crushed with pulverizer keratin, obtains wool keratin powder.
2. the method according to claim 1 for extracting wool keratin from waste wool, which is characterized in that in step (1),
Waste wool is added into the hydrogenperoxide steam generator of 20~24g/L at normal temperatures.
3. the method according to claim 1 for extracting wool keratin from waste wool, which is characterized in that in step (1),
Solution ph is controlled 10.5~11.
4. the method according to claim 1 for extracting wool keratin from waste wool, which is characterized in that in step (1),
Pretreated temperature is 60~90 DEG C, and processing time is 0.5~2 hour.
5. the method according to claim 1 for extracting wool keratin from waste wool, which is characterized in that in step (2),
The addition of alkali protease is 3.2~3.5%o.w.f.
6. the method according to claim 1 for extracting wool keratin from waste wool, which is characterized in that in step (2),
The enzyme activity of alkali protease is 100~110u/mg.
7. the method according to claim 1 for extracting wool keratin from waste wool, which is characterized in that in step (2),
It is first heated to 50~60 DEG C and keeps 45~60min, then proceed to be heated to 80~90 DEG C and keep 45~60min, finally
It is cooled to room temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810643337.2A CN108794614A (en) | 2018-06-21 | 2018-06-21 | The method for extracting wool keratin from waste wool |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810643337.2A CN108794614A (en) | 2018-06-21 | 2018-06-21 | The method for extracting wool keratin from waste wool |
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| CN108794614A true CN108794614A (en) | 2018-11-13 |
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| CN201810643337.2A Pending CN108794614A (en) | 2018-06-21 | 2018-06-21 | The method for extracting wool keratin from waste wool |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110144760A (en) * | 2019-05-21 | 2019-08-20 | 江南大学 | A kind of preparation method of low cost heat-insulating sound-insulating waste wool/PVA extrusion coating paper |
| CN110270316A (en) * | 2018-12-11 | 2019-09-24 | 齐齐哈尔大学 | Decolorising agent and preparation method thereof |
| CN113848102A (en) * | 2021-09-27 | 2021-12-28 | 深圳市心月生物科技有限公司 | Methylamphetamine extracting solution and preparation method thereof |
| CN113855588A (en) * | 2021-11-16 | 2021-12-31 | 上海威之信生物技术有限公司 | Method for preparing keratin solution by wool hydrolysis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565902A (en) * | 2009-04-22 | 2009-10-28 | 江南大学 | Wool-fabric protease anti-felting method based on weak oxidation and cutinase pretreatment |
| CN103497244A (en) * | 2013-09-11 | 2014-01-08 | 昆山市万丰制衣有限责任公司 | Method for extracting keratins from rabbit hair |
-
2018
- 2018-06-21 CN CN201810643337.2A patent/CN108794614A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565902A (en) * | 2009-04-22 | 2009-10-28 | 江南大学 | Wool-fabric protease anti-felting method based on weak oxidation and cutinase pretreatment |
| CN103497244A (en) * | 2013-09-11 | 2014-01-08 | 昆山市万丰制衣有限责任公司 | Method for extracting keratins from rabbit hair |
Non-Patent Citations (1)
| Title |
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| 史吉华等: "碱性蛋白酶法提取废羊毛中角蛋白的机理", 《印染》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110270316A (en) * | 2018-12-11 | 2019-09-24 | 齐齐哈尔大学 | Decolorising agent and preparation method thereof |
| CN110270316B (en) * | 2018-12-11 | 2022-06-24 | 齐齐哈尔大学 | Decoloring agent and preparation method thereof |
| CN110144760A (en) * | 2019-05-21 | 2019-08-20 | 江南大学 | A kind of preparation method of low cost heat-insulating sound-insulating waste wool/PVA extrusion coating paper |
| CN113848102A (en) * | 2021-09-27 | 2021-12-28 | 深圳市心月生物科技有限公司 | Methylamphetamine extracting solution and preparation method thereof |
| CN113855588A (en) * | 2021-11-16 | 2021-12-31 | 上海威之信生物技术有限公司 | Method for preparing keratin solution by wool hydrolysis |
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Application publication date: 20181113 |