CN113826762A - Functional liquid acidifier containing polyhydroxy phenolate and preparation method thereof - Google Patents
Functional liquid acidifier containing polyhydroxy phenolate and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
A functional liquid acidulant containing polyhydroxy phenolate and a method for preparing the same, comprising: water-soluble phenolate, formic acid or buffered formic acid, acetic acid, propionic acid or buffered propionic acid, citric acid, lactic acid, lignosulfonic acid or lignosulfonate and water; the invention has the advantages of scientific compounding, synergistic interaction among the components and multi-functional effect of one agent. The feed additive can increase the feed intake of livestock and poultry and promote growth; meanwhile, the quality of a drinking water system for livestock and poultry can be ensured, the growth and the propagation of harmful bacteria and mould in a water source and a waterline can be inhibited, and a biological film can be removed; the original functional formula is adopted, so that the water body has the functions of water body regulation and stabilization, and is less influenced by inherent environmental facilities; when in actual use, the artificial feed has no pungent smell, and cannot cause negative influence on the original culture system; the finished product is extremely convenient to use and can be directly put into a drinking water system. Extra technical guidance is not needed, and the method is very convenient for technical popularization.
Description
Technical Field
The invention relates to the technical field of processing of drinking water acidulant in animal husbandry, in particular to a functional liquid acidulant containing polyhydroxy phenolate and a preparation method thereof.
Background
Under the large background of 'banning' in the pasturing industry, the acidifier has the characteristics of no residue, no toxic or side effect and no drug resistance, and is recommended to be used as a substitute of antibiotics in more countries and regions at present. Therefore, the use of acidifiers has become a reliable means for improving the feeding environment and raising efficiency in the process of raising livestock. For example, in the aspect of livestock and poultry breeding, the daily water intake of livestock and poultry is usually several times of the food intake, and feeding of nutrient elements or medicines through drinking water is a very convenient and stable mode. In the process of poultry breeding production, the acidulant is added into the drinking water of the poultry, so that the propagation and metabolism of harmful bacteria can be inhibited for a long time, and the water quality cleanliness is improved; meanwhile, the biological membrane in the waterline can be cleaned, the pipeline of the waterline is kept clean, and the drinking water and the ingestion of the livestock and the poultry are not adversely affected. The special functional acidifier added into the drinking water of livestock and poultry can promote the ingestion of the livestock and poultry, thereby promoting the growth of the livestock and poultry. In the aspect of raising the pork pigs, a proper amount of acidifying agents such as potassium butyrate, acid-producing bacterial colonies, natural organic acids and the like are added into drinking water of the pork pigs, so that the diarrhea rate of piglets can be effectively reduced, and the feed weight ratio is improved. The addition of water-soluble acidulant has become a common method for improving the quality of drinking water for livestock and poultry. However, feeding nutritional elements or drugs by drinking water has many advantages, but has extremely high requirements on the water solubility of the optional nutritional elements or drugs.
The physiological activity of 4', 7-dihydroxyisoflavone, Daidzein, as a polyhydroxyphenolic acid, has been demonstrated in many studies. Such as its outstanding estrogenic activity, regulating immunity and resisting arrhythmia. Part of researchers also introduce the application of 4', 7-dihydroxyisoflavone into the field of animal husbandry, and particularly the application of the isoflavone in pig and poultry raising is most widely researched. 4', 7-dihydroxyisoflavone can improve oxidative stress in animals by scavenging free radicals and increasing the active expression of antioxidant proteins in animals; the 4', 7-dihydroxyisoflavone can also effectively promote the growth and the reproduction of animals by regulating the level of partial hormones in the animals; in addition, the 4', 7-dihydroxyisoflavone can also improve the antiviral ability of pigs and poultry and can effectively reduce the fatality rate of PRRSV (porcine reproductive and respiratory syndrome virus) to weaned piglets. The 4', 7-dihydroxyisoflavone has abundant physiological application and definite curative effect, and has the potential of long-term administration. However, 4', 7-dihydroxyisoflavone is a polyhydroxylated phenolic acid structure containing fused rings, and the structural property determines that the structure has poor hydrophilicity and lipophilicity. Therefore, the administration/feeding method is very limited, and the absorption and utilization in the body in the next stage are not ideal. The absolute bioavailability of 4', 7-dihydroxyisoflavone in vivo is only 6.1% (CN 110156736). This property greatly limits the application scenarios and application modes. Therefore, the development of water-soluble 4', 7-dihydroxyisoflavone derivative products is always a research hotspot in the related fields. On the other hand, the phenolic acid structure of 4', 7-dihydroxyisoflavone also determines that it is easily oxidized and deteriorated under alkaline conditions. Therefore, the 4', 7-dihydroxyisoflavone is an excellent combination mode when being compounded with the acidifier.
Disclosure of Invention
In order to overcome the above-mentioned disadvantages of the prior art, it is an object of the present invention to provide a functional liquid acidulant comprising salts of polyhydroxy phenolate and a method for preparing the same.
The technical scheme adopted by the invention for solving the technical problems is as follows: a functional liquid acidulant containing polyhydroxy phenolate comprises the following components in parts by weight:
0.1-10% of water-soluble phenolate, 15-50% of formic acid or buffered formic acid, 2-15% of acetic acid, 2-15% of propionic acid or buffered propionic acid, 5-15% of citric acid, 10-25% of lactic acid, 2-10% of lignosulfonic acid or lignosulfonate and 10-40% of water.
As a further improvement of the invention: the water-soluble phenolate accounts for 0.5-5.5% of the total acidifying agent in percentage by total weight.
As a further improvement of the invention: the liquid acidifying agent is diluted to a pH range of 1-6 when in use.
As a further improvement of the invention: the liquid acidifying agent is diluted to a pH range of 2-5 when in use.
As a further improvement of the invention: the liquid acidifying agent is diluted to a pH range of 3-4 when in use.
As a further improvement of the invention: the pH of the functional liquid acidulant is adjusted using a pH adjuster.
As a further improvement of the invention: the pH regulator is formic acid or buffered formic acid, acetic acid, propionic acid or buffered propionic acid, citric acid, lactic acid, lignosulfonic acid or lignosulfonate and water.
As a further improvement of the invention: a functional liquid acidulant containing polyhydroxy phenolate comprises the following components in parts by weight: 75g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1325g of ultrapure water, 5kg of a functional acidulant containing 5% o of water-soluble phenolate is prepared.
As a further improvement of the invention: a functional liquid acidulant containing polyhydroxy phenolate comprises the following components in parts by weight: 75g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1325g of ultrapure water, 5kg of a functional acidulant containing 1.5% of water-soluble phenolate was prepared.
As a further improvement of the invention: a functional liquid acidulant containing polyhydroxy phenolate comprises the following components in parts by weight: 125g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1275g of ultrapure water, 5kg of a 2.5% aqueous soluble phenolate-containing functional acidulant was prepared.
As a further improvement of the invention: a functional liquid acidulant containing polyhydroxy phenolate comprises the following components in parts by weight: 165g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1235g of ultrapure water, 5kg of a functional acidulant containing 3.5% of water-soluble phenolate was prepared.
As a further improvement of the invention: a functional liquid acidulant containing polyhydroxy phenolate comprises the following components in parts by weight: 225g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1175g of ultrapure water, a 4.5% functional acidulant containing 5kg of water-soluble phenolate was prepared.
A method for preparing functional liquid acidulant containing polyhydroxy phenolate comprises the following steps:
(1) weighing the water-soluble phenolate and the citric acid, adding part of ultrapure water, and dissolving;
(2) weighing the weighed xylonic acid sulfonic acid, adding the rest ultrapure water, and dissolving;
(3) mixing the dissolved water-soluble phenolate, citric acid solution and lignosulfonic acid solution, and uniformly stirring;
(4) sequentially adding weighed formic acid, acetic acid, propionic acid and lactic acid into the mixed solution, and stirring while adding until the mixture is uniformly stirred;
(5) transferring and packaging to obtain the functional liquid acidifier;
(6) when in use, the additive is directly added into drinking water of livestock and poultry according to the required amount.
The use method of the functional liquid acidulant containing the polyhydroxy phenolate comprises the following step of mixing the functional liquid acidulant with drinking water in a weight ratio of 1: 100-1: 5000.
As a further improvement of the invention: the weight ratio of the functional liquid acidifying agent to the drinking water is 1: 500-1: 2000.
As a further improvement of the invention: the weight ratio of the functional liquid acidifier to the drinking water is 1: 800-1: 1200.
Compared with the prior art, the invention has the beneficial effects that:
1) the functional liquid acidifier is scientifically compounded, the components are synergistic, and the effect of one dosage is more than one dosage, so that the functional liquid acidifier can increase the ingestion of livestock and poultry and promote the growth; meanwhile, the quality of a drinking water system for livestock and poultry can be ensured, the growth and the propagation of harmful bacteria and mould in a water source and a waterline can be inhibited, and the biofilm can be removed.
2) The functional liquid acidifier adopts an original functional formula, has the functions of water body regulation and stabilization, and is less influenced by inherent environmental facilities.
3) When the functional liquid acidifier is actually used, the functional liquid acidifier has no pungent smell and cannot cause negative influence on an original culture system.
4) The functional liquid acidifier is extremely convenient to use, can be directly put into a drinking water system, does not need additional technical guidance, and is very convenient for technical popularization.
Drawings
FIG. 1 is a graph of the in vitro free radical scavenging (DPPH.) effect of a water-soluble phenolate salt of a polyhydroxy phenolate salt of a functional liquid acidulant of the present invention in animal feeding experiments.
Detailed Description
In this document, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. The invention will now be further described with reference to the accompanying description and examples:
the first embodiment is as follows:
a functional liquid acidulant containing polyhydroxy phenolate is prepared by preparing 5kg of functional acidulant containing 5 per mill of water-soluble phenolate, wherein the functional acidulant comprises the following components in percentage by weight: 25g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1375g of ultrapure water.
The preparation method and the using method are as follows:
(1) weighing the water-soluble phenolate and the citric acid, adding part of ultrapure water, and dissolving;
(2) weighing the weighed xylonic acid sulfonic acid, adding the rest ultrapure water, and dissolving;
(3) mixing the dissolved water-soluble phenolate, citric acid solution and lignosulfonic acid solution, and uniformly stirring;
(4) sequentially adding weighed formic acid, acetic acid, propionic acid and lactic acid into the mixed solution, and stirring while adding until the mixture is uniformly stirred;
(5) transferring and packaging to obtain the functional liquid acidifier;
(6) when in use, the additive is directly added into drinking water of livestock and poultry according to the required amount.
The second embodiment:
a functional liquid acidulant comprising polyhydroxy phenolate salt, 5kg of a 1.5% functional acidulant containing water soluble phenolate salt is prepared, the percentages by weight including the following components: 75g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1325g of ultrapure water.
The preparation method and the using method are as follows:
(1) weighing the water-soluble phenolate and the citric acid, adding part of ultrapure water, and dissolving;
(2) weighing the weighed xylonic acid sulfonic acid, adding the rest ultrapure water, and dissolving;
(3) mixing the dissolved water-soluble phenolate, citric acid solution and lignosulfonic acid solution, and uniformly stirring;
(4) sequentially adding weighed formic acid, acetic acid, propionic acid and lactic acid into the mixed solution, and stirring while adding until the mixture is uniformly stirred;
(5) transferring and packaging to obtain the functional liquid acidifier;
(6) when in use, the additive is directly added into drinking water of livestock and poultry according to the required amount.
The third embodiment is as follows:
a functional liquid acidulant containing polyhydroxy phenolate is prepared into 5Kg of functional acidulant containing 2.5% of water-soluble phenolate, and the weight percentage comprises the following components: 125g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1275g of ultrapure water.
The preparation method and the using method are as follows:
(1) weighing the water-soluble phenolate and the citric acid, adding part of ultrapure water, and dissolving;
(2) weighing the weighed xylonic acid sulfonic acid, adding the rest ultrapure water, and dissolving;
(3) mixing the dissolved water-soluble phenolate, citric acid solution and lignosulfonic acid solution, and uniformly stirring;
(4) sequentially adding weighed formic acid, acetic acid, propionic acid and lactic acid into the mixed solution, and stirring while adding until the mixture is uniformly stirred;
(5) transferring and packaging to obtain the functional liquid acidifier;
(6) when in use, the additive is directly added into drinking water of livestock and poultry according to the required amount.
The fourth embodiment is as follows:
a functional liquid acidulant comprising polyhydroxy phenolate salt, 5kg of a 3.5% functional acidulant containing water-soluble phenolate salt is prepared, the percentages by weight including the following components: 165g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1235g of ultrapure water.
The preparation method and the using method are as follows:
(1) weighing the water-soluble phenolate and the citric acid, adding part of ultrapure water, and dissolving;
(2) weighing the weighed xylonic acid sulfonic acid, adding the rest ultrapure water, and dissolving;
(3) mixing the dissolved water-soluble phenolate, citric acid solution and lignosulfonic acid solution, and uniformly stirring;
(4) sequentially adding weighed formic acid, acetic acid, propionic acid and lactic acid into the mixed solution, and stirring while adding until the mixture is uniformly stirred;
(5) transferring and packaging to obtain the functional liquid acidifier;
(6) when in use, the additive is directly added into drinking water of livestock and poultry according to the required amount.
The fifth embodiment:
a functional liquid acidulant comprising polyhydroxy phenolate salt, 5kg of a 4.5% functional acidulant containing water-soluble phenolate salt is prepared, the percentages by weight including the following components: 225g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1175g of ultrapure water.
The preparation method and the using method are as follows:
(1) weighing the water-soluble phenolate and the citric acid, adding part of ultrapure water, and dissolving;
(2) weighing the weighed xylonic acid sulfonic acid, adding the rest ultrapure water, and dissolving;
(3) mixing the dissolved water-soluble phenolate, citric acid solution and lignosulfonic acid solution, and uniformly stirring;
(4) sequentially adding weighed formic acid, acetic acid, propionic acid and lactic acid into the mixed solution, and stirring while adding until the mixture is uniformly stirred;
(5) transferring and packaging to obtain the functional liquid acidifier;
(6) when in use, the additive is directly added into drinking water of livestock and poultry according to the required amount.
Animal feeding experiments were performed using functional liquid acidifiers:
720 Wenchang chickens which are 5 weeks old and in the middle chicken stage are selected for the test. The weight was randomly divided into 6 groups of 6 replicates each, and 20 replicates each. In each group, the water drinking system is respectively added with the functional acidulant containing 0, 0.5%, 1.5%, 2.5%, 3.5% and 4.5% of water soluble phenolate, and the ratio of the acidulant to the water drinking is 1: 999. The water source is underground and superficial water inside the farm. The same coarse grain corn-soybean meal type diet was used for all groups. The test period was 21 d.
TABLE 1 pH of functional liquid acidulant-added drinking system
The pH conditions of the blank group and the functional acidulant addition group in each proportion in the feeding experiment are respectively recorded. The result shows that the pH value of a drinking water system is kept between 3 and 4 and is relatively stable after the functional acidulant is added. The range does not affect drinking water of livestock and poultry, can inhibit the breeding of microorganisms in water, and is a more suitable range.
TABLE 2 Chicken weight gain test with addition of functional liquid acidulant
As shown in Table 2, the average daily increase in body weight of the test chickens in the feeding experiment was recorded in the blank group and the functional acidulant-added group at each ratio after the end of the experimental period. From the results, it was found that the functional acidulant of the present invention was diluted 1000 times and added to the drinking water of Wenchang chicken to promote the weight gain. And the effect is most obvious when 2.5 percent of the water-soluble phenolate is added, and the average daily gain is improved by 3.20 percent compared with that of the group without the water-soluble phenolate.
As shown in FIG. 1, the in vitro radical scavenging (DPPH. cndot.) effect of a water-soluble phenolate salt of a polyphenol acid salt was recorded. The results show that the water soluble phenolate has a certain radical scavenging ability. The free radical scavenging effect can reduce the oxidative stress in animals, and is probably the reason for promoting the growth of livestock and poultry.
The main functions of the invention are as follows:
the water-soluble polyhydroxy phenolate provided by the invention is 3 '-sodium sulfonate-4', 7-dihydroxyisoflavone, is a water-soluble derivative (hereinafter referred to as water-soluble phenolate) of 4 ', 7-dihydroxyisoflavone, and the water solubility of the water-soluble polyhydroxy phenolate is improved by thousands of times compared with the water-soluble phenolate of the 4', 7-dihydroxyisoflavone. The water soluble phenolate salt as a derivative of 4 ', 7-dihydroxyisoflavone has a similar physiological activity thereto and has some physiological properties different from those of 4', 7-dihydroxyisoflavone. In addition, the 4', 7-dihydroxyisoflavone derivative has excellent water solubility and acid stability, and is more convenient to be compatible with other feed additives, auxiliaries, carriers and the like. The use scene and the use mode are both expanded sufficiently.
The invention has the advantages of scientific compounding, synergistic interaction among the components and multi-functional effect of one agent. The feed additive can increase the feed intake of livestock and poultry and promote growth; meanwhile, the quality of a drinking water system for livestock and poultry can be ensured, the growth and the propagation of harmful bacteria and mould in a water source and a waterline can be inhibited, and a biological film can be removed; the original functional formula is adopted, so that the water body has the functions of water body regulation and stabilization, and is less influenced by inherent environmental facilities; when in actual use, the artificial feed has no pungent smell, and cannot cause negative influence on the original culture system; the finished product is extremely convenient to use and can be directly put into a drinking water system. Extra technical guidance is not needed, and the method is very convenient for technical popularization.
In summary, after reading the present disclosure, those skilled in the art can make various other corresponding changes without creative mental labor according to the technical solutions and concepts of the present disclosure, and all of them are within the protection scope of the present disclosure.
Claims (10)
1. A functional liquid acidulant containing polyhydroxy phenolate is characterized by comprising the following components in parts by weight:
0.1-10% of water-soluble phenolate, 15-50% of formic acid or buffered formic acid, 2-15% of acetic acid, 2-15% of propionic acid or buffered propionic acid, 5-15% of citric acid, 10-25% of lactic acid, 2-10% of lignosulfonic acid or lignosulfonate and 10-40% of water.
2. The functional liquid acidulant comprising salts of polyhydroxy phenolic acid as claimed in claim 1 wherein said water soluble phenolic acid salts are present in a total percentage of 0.5-5.5% of the total acidulant.
3. The functional liquid acidulant comprising polyhydroxy phenolate as claimed in claim 1, wherein said liquid acidulant is diluted to a pH in the range of 1 to 6 in use.
4. The functional liquid acidulant comprising polyhydroxy phenolate as claimed in claim 3, wherein said functional liquid acidulant is diluted to pH range of 3-4 at the time of use.
5. The functional liquid acidulant comprising polyhydroxy phenolate as claimed in claim 1, wherein the pH of said functional liquid acidulant is adjusted using pH adjusting agent.
6. The functional liquid acidulant comprising salts of polyhydroxy phenolate as claimed in claim 5 wherein said pH adjusting agent is formic acid or buffered formic acid, acetic acid, propionic acid or buffered propionic acid, citric acid, lactic acid, lignosulfonic acid or lignosulfonate and water.
7. The functional liquid acidulant containing polyhydroxy phenolate as claimed in claim 1, comprising the following components in parts by weight: 75g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1325g of ultrapure water.
8. The functional liquid acidulant containing polyhydroxy phenolate as claimed in claim 1, comprising the following components in parts by weight: 125g of water-soluble phenolate, 1500g of formic acid, 250 g of acetic acid, 500g of propionic acid, 500g of citric acid, 750g of lactic acid, 100g of lignosulfonic acid and 1275g of ultrapure water.
9. A method for preparing functional liquid acidulant containing polyhydroxy phenolate is characterized by comprising the following preparation steps:
(1) weighing the water-soluble phenolate and the citric acid, adding part of ultrapure water, and dissolving;
(2) weighing the weighed xylonic acid sulfonic acid, adding the rest ultrapure water, and dissolving;
(3) mixing the dissolved water-soluble phenolate, citric acid solution and lignosulfonic acid solution, and uniformly stirring;
(4) sequentially adding weighed formic acid, acetic acid, propionic acid and lactic acid into the mixed solution, and stirring while adding until the mixture is uniformly stirred;
(5) transferring and packaging to obtain the functional liquid acidifier;
(6) when in use, the additive is directly added into drinking water of livestock and poultry according to the required amount.
10. The use method of the functional liquid acidulant containing the polyhydroxy phenolate is characterized in that the weight ratio of the functional liquid acidulant to drinking water is 1: 100-1: 5000.
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| CN103504137A (en) * | 2013-10-23 | 2014-01-15 | 麦仑(漳州)生物科技有限公司 | Enteric coated fodder acidifying agent and preparation method thereof |
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| CN108617874A (en) * | 2018-05-11 | 2018-10-09 | 广东新南都饲料科技有限公司 | A kind of additive premix reducing sow oxidative stress |
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| CN110122668A (en) * | 2019-06-03 | 2019-08-16 | 烟台金海药业有限公司 | Butcher's beast, domestic animal acylate compound acidulant and preparation method thereof |
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2021
- 2021-09-28 CN CN202111146522.9A patent/CN113826762A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103504137A (en) * | 2013-10-23 | 2014-01-15 | 麦仑(漳州)生物科技有限公司 | Enteric coated fodder acidifying agent and preparation method thereof |
| CN106962612A (en) * | 2016-06-13 | 2017-07-21 | 广东蔚莱生物科技有限公司 | A kind of acidity regulator |
| CN108617874A (en) * | 2018-05-11 | 2018-10-09 | 广东新南都饲料科技有限公司 | A kind of additive premix reducing sow oxidative stress |
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