CN113774082A - 一种核酸表达的方法 - Google Patents

一种核酸表达的方法 Download PDF

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Publication number
CN113774082A
CN113774082A CN202010442805.7A CN202010442805A CN113774082A CN 113774082 A CN113774082 A CN 113774082A CN 202010442805 A CN202010442805 A CN 202010442805A CN 113774082 A CN113774082 A CN 113774082A
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leu
glu
lys
arg
nucleic acid
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CN202010442805.7A
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Chinese (zh)
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谢洪涛
李羽
张洋扬
刘帅
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Shandong Shunfeng Biotechnology Co Ltd
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Shandong Shunfeng Biotechnology Co Ltd
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Priority to CN202010442805.7A priority Critical patent/CN113774082A/zh
Priority to CN202180003994.0A priority patent/CN113994007B/zh
Priority to PCT/CN2021/095310 priority patent/WO2021233442A1/fr
Publication of CN113774082A publication Critical patent/CN113774082A/zh
Pending legal-status Critical Current

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    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04001—Cytosine deaminase (3.5.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04002—Adenine deaminase (3.5.4.2)

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  • Botany (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN202010442805.7A 2020-05-22 2020-05-22 一种核酸表达的方法 Pending CN113774082A (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202010442805.7A CN113774082A (zh) 2020-05-22 2020-05-22 一种核酸表达的方法
CN202180003994.0A CN113994007B (zh) 2020-05-22 2021-05-21 一种核酸表达的方法
PCT/CN2021/095310 WO2021233442A1 (fr) 2020-05-22 2021-05-21 Procédé d'expression d'acide nucléique

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CN202010442805.7A CN113774082A (zh) 2020-05-22 2020-05-22 一种核酸表达的方法

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WO (1) WO2021233442A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115851784B (zh) * 2022-08-02 2023-06-27 安徽农业大学 一种利用Lbcpf1变体构建的植物胞嘧啶碱基编辑系统及其应用
CN117402855B (zh) * 2023-12-14 2024-03-19 中国农业科学院植物保护研究所 一种Cas蛋白、基因编辑系统及应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015007194A1 (fr) * 2013-07-16 2015-01-22 中国科学院上海生命科学研究院 Procédé pour la modification dirigée vers un site de génome de plantes
WO2018039438A1 (fr) * 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation d'acides aminés non naturels dans des protéines au moyen de l'édition de bases
CN106609282A (zh) * 2016-12-02 2017-05-03 中国科学院上海生命科学研究院 一种用于植物基因组定点碱基替换的载体
CN109321584B (zh) * 2017-12-27 2021-07-16 华东师范大学 一种简单定性/定量检测单碱基基因编辑技术工作效率的报告系统
CN110157726B (zh) * 2018-02-11 2023-06-23 中国科学院分子植物科学卓越创新中心 植物基因组定点替换的方法
CN110835634B (zh) * 2018-08-15 2022-07-26 华东师范大学 一种新型碱基转换编辑系统及其应用
CN110527695B (zh) * 2019-03-07 2020-06-16 山东舜丰生物科技有限公司 一种用于基因定点突变的核酸构建物
CN110526993B (zh) * 2019-03-06 2020-06-16 山东舜丰生物科技有限公司 一种用于基因编辑的核酸构建物
CN110129363A (zh) * 2019-06-11 2019-08-16 先正达作物保护股份公司 提高番茄CRISPR/Cas9基因编辑效率的方法

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WO2021233442A1 (fr) 2021-11-25
CN113994007A (zh) 2022-01-28
CN113994007B (zh) 2023-07-04

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