CN113754732A - Small molecular substance for improving cotton yield and fiber quality - Google Patents
Small molecular substance for improving cotton yield and fiber quality Download PDFInfo
- Publication number
- CN113754732A CN113754732A CN202111079997.0A CN202111079997A CN113754732A CN 113754732 A CN113754732 A CN 113754732A CN 202111079997 A CN202111079997 A CN 202111079997A CN 113754732 A CN113754732 A CN 113754732A
- Authority
- CN
- China
- Prior art keywords
- zlc
- cotton
- solution
- yield
- fiber quality
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a small molecular substance ZLC for improving cotton yield and fiber quality, which comprises the effective component of any one or the combination of more than two of small molecular proteins ZLC 1-ZLC 20, wherein the rear 6-position amino acid of the small molecular proteins is SPGMGH. The cotton leaves are sprayed with the aqueous solution containing the micromolecule substance ZLC in the full-bloom stage, so that the nitrogen, phosphorus and potassium absorption capacity of cotton can be enhanced, the growth of the cotton is promoted, and the cotton yield and the fiber quality (fiber length and fiber strength) are improved.
Description
Technical Field
The invention belongs to the technical field of agricultural biology, and particularly relates to a micromolecule substance ZLC for improving cotton yield and fiber quality.
Background
The plants need to absorb major elements such as nitrogen, phosphorus, potassium and the like and trace elements such as iron, magnesium, zinc and the like in the growth process, and the major elements and the trace elements are all necessary for the growth of crops. In agricultural production, fertilizers containing various elements are required to be applied to promote the growth of crops and improve the yield of the crops. The fertilization plays an important role in improving the crop yield, but the fertilizer utilization rate is obviously reduced along with the massive application of the fertilizer, so that the fertilizer is lost, and the environmental pollution is caused. The root system of the crop is a key part for fertilizer absorption, and nutrients such as nitrogen, phosphorus, potassium and the like mainly enter the crop through the root system absorption. Crop roots absorb nutrients from the external environment mainly through different types of transporters. Most nutrient absorption genes are regulated and controlled by external environment (rhizosphere nutrient content, drought, salt stress and the like) and internal factors. Therefore, the nutrient absorption capacity of the root system of the crop is improved by changing the external and internal environments, and the utilization rate of the crop on nutrients can be improved. Photosynthesis plays an important role in plant growth, plant photosynthesis capacity is improved, and plant growth can be promoted by prolonging the photosynthesis time of plant leaves.
The cotton is a crop with strong drought resistance, salt resistance and barren resistance. The cotton has different requirements on fertilizer in different growth periods, and the blooming period is the period of most vigorous fertilizer requirement of the cotton. Therefore, in field production, in order to save labor, fertilization is often performed for 2 times, wherein 1 time is base fertilizer application during sowing, and the other time is topdressing during the flowering stage. However, there is also a significant problem in cotton production in that the fertilizer utilization is reduced by the application of a large amount of fertilizer. On the premise of ensuring that the cotton yield is not reduced, the fertilizer input is reduced, and the improvement of the fertilizer utilization rate is a key problem to be solved in the agricultural production at the present stage.
The plant hormone plays an important regulation role in growth and development of animals and plants and stress tolerance. At present, besides the 6 major phytohormones that are common, many small molecule proteins are also found to have hormone-like functions. At present, a large number of secretory small molecular proteins (polypeptides) are found in plants, and the small molecular proteins can be transported among different tissues and organs and have important functions in the aspects of regulating and controlling plant drought resistance, salt tolerance, plant nutrient absorption and the like. Therefore, on the basis of fully researching the functions of the small molecular proteins, novel small molecular proteins are developed, key small molecular proteins are selected, and a proper in vitro application method is established, so that a brand-new scientific and technological support type strategy method can be provided for improving the utilization rate of crop nutrients and increasing the crop yield.
Disclosure of Invention
The invention aims to find new micromolecular protein for improving cotton yield, increase the content of active micromolecular protein in cotton body by a method of foliage spraying, improve the nutrient absorption capacity of cotton, increase the utilization rate of fertilizer, improve the photosynthesis capacity of cotton, and improve the cotton yield and fiber quality.
Therefore, the technical scheme adopted by the invention is as follows: the micromolecule substance ZLC for improving the cotton yield and the fiber quality is characterized in that the active ingredient is any one or the combination of more than two of 20 micromolecule proteins, and the amino acid sequence is as follows:
ZLC-1：DFRPTTPGHSPGMGH；
ZLC-2：AFRPTTPGHSPGMGH；
ZLC-3：DFRPTNPGNSPGMGH；
ZLC-4：DFRPTTQGHSPGMGH；
ZLC-5：DVRPTAPGHSPGMGH；
ZLC-6：DVRPTTPGHSPGMGN；
ZLC-7：DFMPSGPGHSPGMGH；
ZLC-8：DFPPSGPGHSPGMGH；
ZLC-9：DFRPTAPGHSPGMGH；
ZLC-10：DFQPTTPGHSPGMGH；
ZLC-11：DVHPTPRGHSPGMGH；
ZLC-12：DFRRTSPGHSPGMGH；
ZLC-13：DFRPTTPGNSPGMGH；
ZLC-14：GFRHTTPGNSPGMGH；
ZLC-15：DFRPTTPGSSPGMGH；
ZLC-16：DFQPTAPGNSPGMGH；
ZLC-17：GFRPTTPGNSPGMGH；
ZLC-18：DFKRTSPGHSPGMGH；
ZLC-19：AVRPTKPGHSPGMGH；
ZLC-20：AFRSTTPGHSPGMGH。
further, the ZLC solution is an aqueous solution of ZLC containing Silwet series silicone surfactants. Preferably, Silwet L-77 surfactant is used.
Preferably, the working concentrations of the ZLC solution are: the final concentration of the individual ZLCs or ZLC mixtures is 0.1-0.2g/L (preferably 0.15g/L) and the concentration of the silwet surfactant is 0.005-0.02% (preferably 0.01%). The components are prepared according to the proportion, and are uniformly mixed, and then the sprayer is used for spraying the foliage of the cotton.
The invention also provides a field application method of the ZLC solution, which comprises the following steps:
1) spraying period and dosage: spraying 10-15L ZLC solution per mu in the full-bloom stage of cotton; spraying after 4 pm or in cloudy days without rain;
2) the spraying method comprises the following steps: when in spraying, the front and back sides of the cotton leaves can be uniformly sprayed with the ZLC solution as much as possible;
3) spraying effect: and the ZLC solution is sprayed, so that the growth of cotton can be promoted, the yield of the cotton can be improved, and the length and the strength of cotton fibers can be improved.
The ZLC solution can also be used for crops such as wheat, corn, rice, peanut, soybean, sorghum, millet and the like, can also be used for vegetables and fruits such as Chinese cabbage, garlic, hot pepper, melon, tomato, cucumber, apple and the like, and economic crops such as sesame and the like, and is used for promoting the growth of the crops (vegetables), improving the yield and quality, and improving the absorption and utilization rate of nutrients such as nitrogen, phosphorus, potassium and the like.
The invention has the following beneficial effects:
1. in the prior art, the cotton yield is improved and the fiber quality is improved mainly by conventional breeding or molecular breeding means. The cotton yield and the fiber quality are in negative correlation, and the conventional breeding means has difficulty in improving the cotton yield and the fiber quality at the same time. The method of the invention breaks the limitation of the prior art, small molecular protein is sprayed on leaves, the cotton yield and the fiber quality (fiber length and strength) are improved synergistically, and the nutrient absorption and utilization rate of cotton is greatly improved after the ZLC solution is sprayed, so that the product can reduce the fertilizer consumption, reduce the environmental pollution, improve the cotton yield and quality, and has wide market application prospect.
2. Experiments show that after the fertilizer is sprayed on the leaf surfaces of a cotton field, the fertilizer can obviously improve the content of nutrients such as nitrogen, phosphorus, potassium and the like in cotton bodies, can promote the growth of cotton, and can improve the yield and the quality of the cotton. Therefore, the method of the invention not only can improve the utilization rate of the cotton fertilizer, but also has the function of improving the yield and quality of cotton, and provides a practical and effective product and method for weight-reducing and efficiency-increasing of cotton and promoting the sustainable development of cotton industry.
3. The micromolecule protein sequence of the invention is verified by a large number of experiments of the inventor of the application, and the small molecule protein can be promoted to be absorbed and utilized by plants by adding silwet in ZLC, so that the nutrient absorption of cotton is improved, and the yield and the quality of cotton are improved.
Drawings
FIG. 1 shows the effect of ZLC solution treatment on cotton growth and yield in experimental examples. Note: CK is spraying clear water as contrast, and ZLC is spraying ZLC solution.
FIG. 2 is a graph showing the effect of dual-purpose ZLC solution treatment on cotton nutrient content in experimental examples. Note: CK is spraying clear water as contrast, and ZLC is spraying ZLC solution.
FIG. 3 shows the results of the effect of the three-way ZLC solution treatment on cotton fiber quality in the experimental examples. Note: CK is spraying clear water as contrast, and ZLC is spraying ZLC solution.
Detailed Description
The technical solution and the technical effects thereof are further explained in the following with reference to the specific embodiments and the accompanying drawings, and the following description is only for explaining the present invention, but not limiting the present invention in any way, and any changes or substitutions based on the present invention are within the protection scope of the present invention. The methods of the present invention are conventional in the art unless otherwise specified. The reagents of the present invention are commercially available unless otherwise specified.
Example 1: determination and synthesis of amino acid sequence of small molecule protein
(1) Determination of amino acid sequence of small molecule protein
The inventor of the application carries out related analysis and research of bioinformatics according to the protein sequence in various plants (including cotton) and by combining the expression abundance of the protein gene in the cotton, changes the 13 th protein into methionine (M), and finds that the function of the small molecular protein can be obviously improved after the 13 th protein is changed into the methionine. Therefore, 20 small molecule protein sequences were finally determined. The last 6 amino acids of the 20 small molecule proteins are conserved as follows: SPGMGH. The 20 small molecule protein sequences are named as: ZLC-1 to ZLC-20. Remarking: ZLC stands for wisdom (Zhi hui), green (Lv se), Cotton (Cotton).
(2) Small molecule protein synthesis and preservation
Synthesizing the needed small molecular protein by using a solid phase synthesis method, and purifying by using a reverse high performance liquid chromatography and a low temperature freeze drying method to obtain the small molecular protein with the purity of more than 95%. The small molecular protein can be stored at-80 deg.C for about 2 years, and at-20 deg.C for about half a year.
Example 2: preparation and application of foliar fertilizer ZLC
Dissolving 15g ZLC (single substance or mixture) with 1L deionized water to obtain 100x mother solution, and standing the mother solution at-20 deg.C for 1-2 weeks, if needed at room temperature.
When the surfactant is applied to fields, 100ml of ZLC mother liquor is diluted by tap water, the final dilution volume is up to 10L, then 1ml of silwet L-77 surfactant is added and uniformly mixed, the final concentration of the surfactant (single ZLC or ZLC mixture) is up to 0.15g/L, and the ZLC working solution contains 1/10000 of the silwet L-77 surfactant.
Spraying the foliage of the cotton in the full-bloom stage, wherein the spraying amount is 10-15L/mu; the spraying is carried out after 4 pm or in cloudy days without rain. When the agent is sprayed, the front and back sides of the cotton leaves can be uniformly sprayed with the ZLC solution as much as possible.
Test example I, influence of foliar spray of ZLC solution on growth and yield of field cotton
And (4) sowing the filled and plump cotton seeds K836 in the field in 2020, 4 months and 30 days. 20 treatments of spraying ZLC (20 types of ZLC-1 to ZLC-20 in total) solutions were set, and the treatments were repeated 3 times in random block arrangement, with clear water sprayed with silwet L-77 surfactant of the same concentration as a control. Cell area 30m2The method adopts 4-row area, and plants are planted at equal row spacing, the row spacing is 76cm, and the planting spacing is 15 cm. And performing daily management on cotton according to local cotton planting habits. In the full-bloom stage, after 4 pm, 15L of ZLC solution of 0.15g/L is sprayed on each mu of leaf surface, and clear water is sprayed as a control. In the boll opening period, cotton is harvested, and the biological yield and the seed cotton yield of the cotton are measured. The ZLC solutions in this experiment were 20 kinds of small molecule protein solutions containing ZLC-1 to ZLC-20, respectively (the preparation method is shown in example 2).
And (3) test results: cotton was harvested at the boll opening stage (10 months and 11 days), biomass and seed cotton yield were measured, and it was found that spraying ZLC significantly increased cotton biological yield (fig. 1A) and seed cotton yield (fig. 1B) compared to the control. The fact that the leaf surface spraying of ZLC can obviously promote the growth of cotton and improve the yield of the cotton is proved.
Test example II, influence of foliar spraying of ZLC solution on nutrient absorption and utilization of field cotton
And (4) sowing the filled and plump cotton seeds K836 in the field in 2020, 4 months and 30 days. 20 treatments of spraying ZLC (20 types of ZLC-1 to ZLC-20 in total) solutions were set, and the treatments were repeated 3 times in random block arrangement, with clear water sprayed with silwet L-77 surfactant of the same concentration as a control. Cell area 30m2The method adopts 4-row area, and plants are planted at equal row spacing, the row spacing is 76cm, and the planting spacing is 15 cm. And performing daily management on cotton according to local cotton planting habits. In the full-bloom stage, after 4 pm, 15L of ZLC solution of 0.15g/L is sprayed on each mu of leaf surface, and clear water is sprayed as a control. And (4) harvesting cotton in a boll opening period, and measuring the nitrogen, potassium and phosphorus contents absorbed by the cotton. The ZLC solutions in this experiment were 20 kinds of small molecule protein solutions containing ZLC-1 to ZLC-20, respectively (the preparation method is shown in example 2).
And (3) test results: cotton is harvested in the boll opening period (10 months and 11 days), the nitrogen, potassium and phosphorus absorbed by the cotton are measured, and the nitrogen (figure 2A), potassium (figure 2B) and phosphorus (figure 2C) absorbed by the cotton are obviously improved by spraying the ZLC compared with a control.
Experimental example influence of trilobate spraying ZLC solution on field cotton fiber quality
And (3) sowing the full and full cotton seeds K836 to the Amelanchier-free field in 2020, 4 and 30 days. 20 treatments of spraying ZLC (20 types of ZLC-1 to ZLC-20 in total) solutions were set, and the treatments were repeated 3 times in random block arrangement, with clear water sprayed with silwet L-77 surfactant of the same concentration as a control. Cell area 30m2The method adopts 4-row area, and plants are planted at equal row spacing, the row spacing is 76cm, and the planting spacing is 15 cm. And performing daily management on cotton according to local cotton planting habits. In the full-bloom stage, after 4 pm, 15L of ZLC solution of 0.15g/L is sprayed on each mu of leaf surface, and clear water is sprayed as a control. During the boll opening period, cotton is harvested and the cotton fiber quality is determined. The ZLC solutions in this experiment were 20 kinds of small molecule protein solutions containing ZLC-1 to ZLC-20, respectively (the preparation method is shown in example 2).
And (3) test results: cotton was harvested during the boll opening period (10 months and 11 days), cotton fiber quality was determined, and it was found that spraying ZLC significantly increased cotton fiber length (fig. 3A) and fiber strength (fig. 3B) compared to the control.
Claims (6)
1. A micromolecule substance ZLC for improving cotton yield and fiber quality is characterized in that the active ingredient is any one of or the combination of more than two of 20 micromolecule proteins;
the amino acid sequence of the small molecule protein is as follows:
ZLC-1：DFRPTTPGHSPGMGH；
ZLC-2：AFRPTTPGHSPGMGH；
ZLC-3：DFRPTNPGNSPGMGH；
ZLC-4：DFRPTTQGHSPGMGH；
ZLC-5：DVRPTAPGHSPGMGH；
ZLC-6：DVRPTTPGHSPGMGN；
ZLC-7：DFMPSGPGHSPGMGH；
ZLC-8：DFPPSGPGHSPGMGH；
ZLC-9：DFRPTAPGHSPGMGH；
ZLC-10：DFQPTTPGHSPGMGH；
ZLC-11：DVHPTPRGHSPGMGH；
ZLC-12：DFRRTSPGHSPGMGH；
ZLC-13：DFRPTTPGNSPGMGH；
ZLC-14：GFRHTTPGNSPGMGH；
ZLC-15：DFRPTTPGSSPGMGH；
ZLC-16：DFQPTAPGNSPGMGH；
ZLC-17：GFRPTTPGNSPGMGH；
ZLC-18：DFKRTSPGHSPGMGH；
ZLC-19：AVRPTKPGHSPGMGH；
ZLC-20：AFRSTTPGHSPGMGH。
2. a small molecule substance ZLC solution for improving cotton yield and fiber quality, which is characterized in that the effective component is any one or the combination of more than two of the 20 small molecule proteins in the claim 1, and the ZLC solution is a ZLC aqueous solution containing Silwet series organosilicon surfactants.
3. The small molecule substance ZLC solution for improving cotton yield and fiber quality as claimed in claim 2, wherein said ZLC solution has working concentrations of: the final concentration of the single ZLC or ZLC mixture is 0.1-0.2g/L and the concentration of the silwet surfactant is 0.005-0.02%.
4. The small molecule substance ZLC solution for improving cotton yield and fiber quality of claim 3, wherein said ZLC solution has working concentrations of: the final concentration of the individual ZLCs or ZLC mixtures was 0.15g/L and the concentration of the silwet surfactant was 0.01%.
5. The field application method of ZLC solution as claimed in claims 1-4 wherein the ZLC solution is sprayed at full bloom stage of cotton at 10-15L/acre; the spraying is carried out after 4 pm or in cloudy days without rain.
6. Use of a ZLC solution according to any one of claims 1 to 4 for the preparation of products for improving the yield, quality and nutrient absorption of cotton, other crops and vegetables.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111079997.0A CN113754732B (en) | 2021-09-15 | 2021-09-15 | Small molecular substance for improving cotton yield and fiber quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111079997.0A CN113754732B (en) | 2021-09-15 | 2021-09-15 | Small molecular substance for improving cotton yield and fiber quality |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113754732A true CN113754732A (en) | 2021-12-07 |
| CN113754732B CN113754732B (en) | 2023-04-25 |
Family
ID=78795765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111079997.0A Active CN113754732B (en) | 2021-09-15 | 2021-09-15 | Small molecular substance for improving cotton yield and fiber quality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113754732B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114478705A (en) * | 2022-02-24 | 2022-05-13 | 山东省农业科学院 | Small molecular protein for improving photosynthesis of wheat and application thereof |
| CN114507271A (en) * | 2022-02-24 | 2022-05-17 | 山东省农业科学院 | Small molecular protein for improving tobacco leaf quality |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245489A (en) * | 2017-06-08 | 2017-10-13 | 山东农业大学 | A kind of apple polypeptide hormone gene M dCEP7 of regulation and control root system development and its application |
| CN110063332A (en) * | 2019-05-21 | 2019-07-30 | 山东棉花研究中心 | It is a kind of improve root system of plant NO3--N translocation promotor and application |
| CN111620935A (en) * | 2019-02-27 | 2020-09-04 | 中国农业大学 | Application of ZmCEP1 gene in regulation and control of corn kernel development |
-
2021
- 2021-09-15 CN CN202111079997.0A patent/CN113754732B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245489A (en) * | 2017-06-08 | 2017-10-13 | 山东农业大学 | A kind of apple polypeptide hormone gene M dCEP7 of regulation and control root system development and its application |
| CN111620935A (en) * | 2019-02-27 | 2020-09-04 | 中国农业大学 | Application of ZmCEP1 gene in regulation and control of corn kernel development |
| CN110063332A (en) * | 2019-05-21 | 2019-07-30 | 山东棉花研究中心 | It is a kind of improve root system of plant NO3--N translocation promotor and application |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114478705A (en) * | 2022-02-24 | 2022-05-13 | 山东省农业科学院 | Small molecular protein for improving photosynthesis of wheat and application thereof |
| CN114507271A (en) * | 2022-02-24 | 2022-05-17 | 山东省农业科学院 | Small molecular protein for improving tobacco leaf quality |
| CN114478705B (en) * | 2022-02-24 | 2023-05-05 | 山东省农业科学院 | Small molecular protein for improving photosynthesis of wheat and application thereof |
| CN114507271B (en) * | 2022-02-24 | 2023-06-09 | 山东省农业科学院 | Small molecular protein for improving tobacco leaf quality |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113754732B (en) | 2023-04-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108934851B (en) | Saline-alkali-tolerant cold-area stress-resistant rice seedling raising method | |
| CN102285841B (en) | Fertilizer special for bamboo and production method thereof | |
| CN103980052A (en) | Special fertilizer for fruit trees and preparation method thereof | |
| CN101953249B (en) | Fertilization method for protected tomato overwinter long-season cultivation | |
| CN113754732B (en) | Small molecular substance for improving cotton yield and fiber quality | |
| CN110698256A (en) | Water-soluble fertilizer containing fish protein | |
| CN107155550B (en) | Thick-skin melon cultivation fertilizing method | |
| CN106957201A (en) | Tea oil tree special-purpose foliage fertilizer and preparation method thereof | |
| CN104108989B (en) | Growth regulator dedicated and the application of a kind of kyoto grape | |
| CN111943752A (en) | Fertilizer capable of remarkably improving quality and yield of vegetables | |
| CN103314754B (en) | Sweet potato cultivation method using whole-course chemical control | |
| CN112409058A (en) | Preparation method of amino acid-containing water-soluble fertilizer | |
| CN108901507B (en) | Method for preventing and treating cotton verticillium wilt | |
| CN111763115B (en) | Fertilizer synergist and preparation method and application thereof | |
| CN113754489A (en) | Multi-effect foliar fertilizer and preparation method thereof | |
| CN105294311A (en) | Honeysuckle compound leaf fertilizer and preparation method thereof | |
| CN103694030B (en) | A kind of fruit retention agent for preventing drop that prevents persimmon fruit drop containing AVG | |
| CN107827520A (en) | A kind of change controlling agent for improving wheat yield and change prosecutor method | |
| CN107556077B (en) | Special base fertilizer and additional fertilizer for grapes suitable for integration of water and fertilizer in southwest region | |
| CN113475332A (en) | Method for spraying magnesium fertilizer on rice leaf surfaces | |
| CN110981588A (en) | Novel liquid rooting agent and preparation method thereof | |
| CN110317104A (en) | A kind of soil conditioner and method of administration suitable for facilities horticulture protecting field | |
| WO2006075675A1 (en) | Novel method of cultivating fruit vegetable crop under low nutritional condition | |
| Shcatula et al. | Features of application of mineral fertilizers in the growing of winter barley | |
| CN108546179A (en) | Promoting root growth, anti-lopsided block root crop fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |