CN113754732B - Small molecular substance for improving cotton yield and fiber quality - Google Patents
Small molecular substance for improving cotton yield and fiber quality Download PDFInfo
- Publication number
- CN113754732B CN113754732B CN202111079997.0A CN202111079997A CN113754732B CN 113754732 B CN113754732 B CN 113754732B CN 202111079997 A CN202111079997 A CN 202111079997A CN 113754732 B CN113754732 B CN 113754732B
- Authority
- CN
- China
- Prior art keywords
- zlc
- cotton
- solution
- small molecular
- yield
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a small molecular substance ZLC for improving cotton yield and fiber quality, which comprises any one or more than two of small molecular proteins ZLC 1-ZLC 20 as active ingredients, wherein the last 6 amino acids of the small molecular proteins are SPGMGH. The cotton leaves are sprayed in the full bloom stage by adopting the aqueous solution containing the small molecular substance ZLC, so that the nitrogen, phosphorus and potassium absorption capacity of cotton can be enhanced, the cotton growth can be promoted, and the cotton yield and the fiber quality (fiber length and fiber strength) can be improved.
Description
Technical Field
The invention belongs to the technical field of agricultural biology, and particularly relates to a small molecular substance ZLC for improving cotton yield and fiber quality.
Background
The plant needs to absorb major elements such as nitrogen, phosphorus, potassium and the like and trace elements such as iron, magnesium, zinc and the like in the growth process, and the major elements and the trace elements are all necessary for the growth of crops. In agricultural production, it is necessary to apply fertilizers containing various elements, promote crop growth, and increase crop yield. Fertilization plays an important role in improving crop yield, but with the massive application of fertilizer, the fertilizer utilization rate is obviously reduced, so that fertilizer loss is caused, and environmental pollution is caused. The root system of the crop is a key part for absorbing fertilizer, and nitrogen, phosphorus, potassium and other nutrients are mainly absorbed into the crop through the root system. Crop roots absorb nutrients from the external environment mainly through different types of transport bodies. Most nutrient absorption genes are regulated and controlled by external environment (how much rhizosphere nutrient is, drought, salt stress and the like) and internal factors. Therefore, the nutrient absorption capacity of the root system of the crop is improved by changing the external environment and the internal environment, and the nutrient utilization rate of the crop can be improved. Photosynthesis plays an important role in plant growth, improves the photosynthesis capacity of plants, prolongs the photosynthesis time of plant leaves, and can promote plant growth.
Cotton is a crop with strong drought resistance, salt tolerance and barren tolerance. The cotton has different fertilizer requirements in different growth periods, and the full-bloom period is the period in which the cotton needs the most vigorous fertilizer. Therefore, in field production, 2 times of fertilization are usually performed for saving labor, 1 time of applying base fertilizer during sowing and another time of topdressing during the flower bell period. However, there is also a significant problem in cotton production that a large amount of fertilizer is applied, resulting in a decrease in fertilizer utilization. On the premise of ensuring that the cotton yield is not reduced, the fertilizer input is reduced, and the improvement of the fertilizer utilization rate is a key problem to be solved in the current-stage agricultural production.
The plant hormone plays an important role in regulating and controlling the growth and development of animals and plants and stress tolerance. At present, in addition to the common 6 major classes of plant hormones, many small molecule proteins have also been found to function like hormones. At present, a large number of secreted small molecular proteins (polypeptides) are found in plants, and the small molecular proteins can be transported between different tissues and organs, and have important functions in the aspects of regulating drought resistance and salt tolerance of plants, regulating nutrient absorption of plants and the like. Therefore, on the basis of fully researching the functions of the small molecular proteins, novel small molecular proteins are developed, key small molecular proteins are selected, and a proper in-vitro application method is established, so that a brand-new technological support strategy method is provided for improving the nutrient utilization rate of crops and the yield of crops.
Disclosure of Invention
The invention aims to find novel small molecular proteins for improving cotton yield, increase the content of active small molecular proteins in cotton by a foliar spraying method, improve the nutrient absorption capacity of cotton, increase the fertilizer utilization rate, improve the photosynthesis capacity of cotton and improve the cotton yield and fiber quality.
The technical scheme adopted by the invention is as follows: a small molecular substance ZLC for improving cotton yield and fiber quality is characterized in that the effective component is any one or the combination of more than two of 20 small molecular proteins, and the amino acid sequence is as follows:
ZLC-1：DFRPTTPGHSPGMGH；
ZLC-2：AFRPTTPGHSPGMGH；
ZLC-3：DFRPTNPGNSPGMGH；
ZLC-4：DFRPTTQGHSPGMGH；
ZLC-5：DVRPTAPGHSPGMGH；
ZLC-6：DVRPTTPGHSPGMGN；
ZLC-7：DFMPSGPGHSPGMGH；
ZLC-8：DFPPSGPGHSPGMGH；
ZLC-9：DFRPTAPGHSPGMGH；
ZLC-10：DFQPTTPGHSPGMGH；
ZLC-11：DVHPTPRGHSPGMGH；
ZLC-12：DFRRTSPGHSPGMGH；
ZLC-13：DFRPTTPGNSPGMGH；
ZLC-14：GFRHTTPGNSPGMGH；
ZLC-15：DFRPTTPGSSPGMGH；
ZLC-16：DFQPTAPGNSPGMGH；
ZLC-17：GFRPTTPGNSPGMGH；
ZLC-18：DFKRTSPGHSPGMGH；
ZLC-19：AVRPTKPGHSPGMGH；
ZLC-20：AFRSTTPGHSPGMGH。
further, the ZLC solution is an aqueous ZLC solution containing a Silwet series of silicone surfactants. A Silwet L-77 surfactant is preferably used.
Preferably, the working concentration of the ZLC solution is: the final concentration of the single ZLC or ZLC mixture is 0.1-0.2g/L (preferably 0.15 g/L) and the concentration of silwet surfactant is 0.005-0.02% (preferably 0.01%). The cotton leaf surface spraying agent is prepared according to the proportion, and the cotton leaf surface spraying agent is sprayed on the cotton leaf surface by using a sprayer after being uniformly mixed.
The invention also provides a field application method of the ZLC solution, which comprises the following steps:
1) Spraying period and dosage: spraying 10-15L of ZLC solution per mu in the cotton full bloom period; spraying after 4 pm or in cloudy days without rain;
2) The spraying method comprises the following steps: when in spraying, ZLC solution can be uniformly sprayed on the front and back surfaces of cotton leaves as much as possible;
3) The spraying effect is as follows: the ZLC solution is sprayed, so that the growth of cotton can be promoted, the yield of cotton can be improved, and the length and strength of cotton fibers can be improved.
The ZLC solution of the invention can be used for crops such as wheat, corn, rice, peanut, soybean, sorghum, millet and the like, vegetables and fruit plants such as cabbage, garlic, capsicum, melon, tomato, cucumber, apple and the like, and economic crops such as sesame and the like, and can be used for promoting the growth of the crops (vegetables), improving the yield and the quality, and improving the nutrient absorption and utilization rates of nitrogen, phosphorus, potassium and the like.
The invention has the following beneficial effects:
1. the prior art mainly improves cotton yield and fiber quality through conventional breeding or molecular breeding means. Cotton yield and fiber quality are inversely related, and it is difficult for conventional breeding means to increase both cotton yield and fiber quality. The method breaks the limitation of the prior art, adopts the blade to spray the small molecular protein, cooperatively improves the cotton yield and the fiber quality (improves the fiber length and the fiber strength), and greatly improves the nutrient absorption and utilization rate of the cotton after the ZLC solution is sprayed, so that the product can reduce the fertilizer consumption, reduce the environmental pollution, improve the cotton yield and the cotton quality, and has wide market application prospect.
2. Experiments show that after the large Tian Shemian cotton is sprayed, the nutrient contents of nitrogen, phosphorus, potassium and the like in cotton can be obviously improved, the growth of cotton can be promoted, the yield of cotton can be improved, and the quality of cotton can be improved. Therefore, the method of the invention not only can improve the utilization rate of cotton fertilizer, but also has the function of improving the yield and quality of cotton, and provides a realistic and effective product and method for reducing weight and enhancing efficiency of cotton and promoting sustainable development of cotton industry.
3. The invention discloses a method for preparing a small molecular protein sequence, which is verified by a large number of experiments of the inventor of the application, and the addition of silwet in ZLC can promote plant absorption and utilization of the small molecular protein, so that the nutrient absorption of cotton is improved, and the yield and quality of cotton are improved.
Drawings
FIG. 1 is a graph showing the results of a test example of the effect of treating cotton with ZLC solution on cotton growth and yield. And (3) injection: CK is the spray water control, ZLC is the spray ZLC solution treatment.
FIG. 2 is a graph showing the effect of treating cotton with ZLC solution on cotton nutrient content for test example two. And (3) injection: CK is the spray water control, ZLC is the spray ZLC solution treatment.
FIG. 3 is a graph showing the results of a test example of the effect of a three-purpose ZLC solution on cotton fiber quality when treating cotton. And (3) injection: CK is the spray water control, ZLC is the spray ZLC solution treatment.
Detailed Description
The technical scheme of the present invention and the technical effects thereof are further described below with reference to specific embodiments and drawings, the following description is only for explaining the present invention, but not limiting the present invention in any way, and any changes or substitutions based on the present invention are all within the scope of the present invention. The method of the invention is a conventional method in the art unless specifically stated otherwise. The reagents of the invention are commercially available unless otherwise specified.
Example 1: determination and synthesis of amino acid sequence of small molecular protein
(1) Determination of amino acid sequence of small molecule protein
The inventor of the application performs biological informatics related analysis and research according to the protein sequence in various plants (including cotton) and the expression abundance of the protein gene in the cotton, and changes the 13 th protein into methionine (M), and can obviously improve the function of the small molecular protein after finding that the 13 th protein is changed into methionine. Thus, 20 small molecule protein sequences were ultimately determined. The last 6 th amino acid of the 20 small molecule proteins is conserved, as follows: SPGMGH. The 20 small molecule protein sequences were respectively named: ZLC-1 to ZLC-20. Remarks: ZLC stands for wisdom (Zhi hui), green (Lv se), cotton (Cotton).
(2) Synthesis and preservation of small molecule proteins
The method comprises the steps of synthesizing the needed small molecular protein by using a solid phase synthesis method, and purifying by using a reverse high performance liquid chromatography and a low temperature freeze drying method to obtain the small molecular protein with the purity of more than 95%. The small molecular proteins can be stored at-80deg.C for about 2 years and at-20deg.C for about half a year.
Example 2: preparation and application of foliar fertilizer ZLC
15g of ZLC (single substance or mixture) is taken and dissolved by 1L of deionized water to prepare 100x mother liquor, and the mother liquor can be placed below-20 ℃ for 1-2 weeks, and is prepared at room temperature for use.
When the method is applied to fields, 100ml of ZLC mother liquor is taken, diluted by tap water, finally diluted to a constant volume of 10L, then 1ml of silwet L-77 surfactant is added, and the mixture is uniformly mixed, so that the final concentration of the ZLC mother liquor (single ZLC or ZLC mixture) reaches 0.15g/L, and the ZLC working liquor contains 1/10000 of silwet L-77 surfactant.
Spraying leaves in the cotton full-bloom period, wherein the spraying amount is 10-15L/mu; spraying after 4 pm or in cloudy days without rain. When in spraying, ZLC solution can be uniformly sprayed on the front and back surfaces of cotton leaves as much as possible.
Test example one, influence of ZLC solution sprayed on leaf surface on field cotton growth and yield
Full cotton seeds K836 were sown in the field at 30/4/2020. The treatment of 20 ZLC (ZLC-1 to ZLC-20 total of 20 types) solutions was set, and the spray of clear water containing the same concentration of silwet L-77 surfactant was used as a control, and the arrangement of the groups was random and repeated 3 times. Cell area 30m 2 4 rows of areas are adopted, the planting is carried out at equal row spacing, the row spacing is 76cm, and the plant spacing is 15cm. And carrying out daily management on cotton according to the habit of local cotton planting. In the full bloom stage, after 4 pm, ZLC solution of 0.15g/L is sprayed on each mu of leaf surface for 15L, and water is sprayed as a control. During the opening period, cotton was harvested and cotton biomass and seed cotton yield were measured. The ZLC solutions in this test were 20 small molecule protein solutions containing ZLC-1 through ZLC-20, respectively (the preparation method is shown in example 2).
Test results: cotton was harvested during the cotton opening period (10 months 11 days), biomass and seed cotton yield were measured, and ZLC was found to significantly increase cotton biomass yield (FIG. 1A) and seed cotton yield (FIG. 1B) when sprayed as compared to the control. Proved by the application of ZLC on leaf surfaces, the growth of cotton can be obviously promoted, and the cotton yield can be improved.
Test example II, influence of ZLC solution sprayed on leaf surface on nutrient absorption and utilization of field cotton
Full cotton seeds K836 were sown in the field at 30/4/2020. The treatment of 20 ZLC (ZLC-1 to ZLC-20 total of 20 types) solutions was set, and the spray of clear water containing the same concentration of silwet L-77 surfactant was used as a control, and the arrangement of the groups was random and repeated 3 times. Cell area 30m 2 4 rows of areas are adopted, the planting is carried out at equal row spacing, the row spacing is 76cm, and the plant spacing is 15cm. And carrying out daily management on cotton according to the habit of local cotton planting. In the full bloom stage, after 4 pm, ZLC solution of 0.15g/L is sprayed on each mu of leaf surface for 15L, and water is sprayed as a control. During the boll opening period, cotton is harvested and the nitrogen, potassium and phosphorus content absorbed by the cotton is measured. The ZLC solutions in this test were 20 small molecule protein solutions containing ZLC-1 through ZLC-20, respectively (the preparation method is shown in example 2).
Test results: cotton was harvested during the cotton opening period (10 months 11 days), and the nitrogen, potassium, and phosphorus content of the cotton was measured, and it was found that spraying ZLC significantly increased the nitrogen (fig. 2A), potassium (fig. 2B), and phosphorus (fig. 2C) content of the cotton compared to the control.
Test example Effect of three-blade surface ZLC solution spraying on field Cotton fiber quality
Full cotton K836 seed was sown in the chiselless field at 30/4/2020. The treatment of 20 ZLC (ZLC-1 to ZLC-20 total of 20 types) solutions was set, and the spray of clear water containing the same concentration of silwet L-77 surfactant was used as a control, and the arrangement of the groups was random and repeated 3 times. Cell area 30m 2 4 rows of areas are adopted, the planting is carried out at equal row spacing, the row spacing is 76cm, and the plant spacing is 15cm. And carrying out daily management on cotton according to the habit of local cotton planting. In the full bloom stage, after 4 pm, ZLC solution of 0.15g/L is sprayed on each mu of leaf surface for 15L, and water is sprayed as a control. During the opening period, cotton is harvested and the cotton fiber quality is determined. The ZLC solutions in this test were 20 small molecule protein solutions containing ZLC-1 through ZLC-20, respectively (the preparation method is shown in example 2).
Test results: cotton was harvested during the cotton opening period (10 months 11 days) and cotton fiber quality was measured and ZLC was found to significantly increase cotton fiber length (FIG. 3A) and fiber strength (FIG. 3B) when compared to the control.
Claims (6)
1. A small molecular substance ZLC for improving cotton yield and fiber quality is characterized in that the effective component is any one or more than two of 20 small molecular proteins;
the amino acid sequence of the small molecule protein is as follows:
ZLC-1：DFRPTTPGHSPGMGH；
ZLC-2：AFRPTTPGHSPGMGH；
ZLC-3：DFRPTNPGNSPGMGH；
ZLC-4：DFRPTTQGHSPGMGH；
ZLC-5：DVRPTAPGHSPGMGH；
ZLC-6：DVRPTTPGHSPGMGN；
ZLC-7：DFMPSGPGHSPGMGH；
ZLC-8：DFPPSGPGHSPGMGH；
ZLC-9：DFRPTAPGHSPGMGH；
ZLC-10：DFQPTTPGHSPGMGH；
ZLC-11：DVHPTPRGHSPGMGH；
ZLC-12：DFRRTSPGHSPGMGH；
ZLC-13：DFRPTTPGNSPGMGH；
ZLC-14：GFRHTTPGNSPGMGH；
ZLC-15：DFRPTTPGSSPGMGH；
ZLC-16：DFQPTAPGNSPGMGH；
ZLC-17：GFRPTTPGNSPGMGH；
ZLC-18：DFKRTSPGHSPGMGH；
ZLC-19：AVRPTKPGHSPGMGH；
ZLC-20：AFRSTTPGHSPGMGH。
2. the small molecular substance ZLC solution for improving cotton yield and fiber quality is characterized in that the effective component is any one or more than two of 20 small molecular proteins in the claim 1, and the ZLC solution is an aqueous solution of ZLC containing Silwet series organosilicon surfactant.
3. The small molecule substance ZLC solution according to claim 2, wherein the working concentration of the ZLC solution is: the final concentration of single ZLC or ZLC mixture is 0.1-0.2g/L, and the volume concentration of silwet surfactant is 0.005-0.02%.
4. A small molecule substance ZLC solution for increasing cotton yield and fiber quality as defined in claim 3, wherein said ZLC solution has a working concentration of: the final concentration of the single ZLC or ZLC mixture was 0.15g/L and the volume concentration of silwet surfactant was 0.01%.
5. The method for using the ZLC solution in the field according to any of the claims 2 to 4, wherein the ZLC solution is sprayed in the cotton full bloom period, 10 to 15 and L per mu; spraying after 4 pm or in cloudy days without rain.
6. Use of the ZLC solution of any of claims 2 to 4 for the preparation of a product that increases the yield, quality and nutrient absorption of cotton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111079997.0A CN113754732B (en) | 2021-09-15 | 2021-09-15 | Small molecular substance for improving cotton yield and fiber quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111079997.0A CN113754732B (en) | 2021-09-15 | 2021-09-15 | Small molecular substance for improving cotton yield and fiber quality |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113754732A CN113754732A (en) | 2021-12-07 |
| CN113754732B true CN113754732B (en) | 2023-04-25 |
Family
ID=78795765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111079997.0A Active CN113754732B (en) | 2021-09-15 | 2021-09-15 | Small molecular substance for improving cotton yield and fiber quality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113754732B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114478705B (en) * | 2022-02-24 | 2023-05-05 | 山东省农业科学院 | Small molecular protein for improving photosynthesis of wheat and application thereof |
| CN114507271B (en) * | 2022-02-24 | 2023-06-09 | 山东省农业科学院 | Small molecular protein for improving tobacco leaf quality |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245489A (en) * | 2017-06-08 | 2017-10-13 | 山东农业大学 | A kind of apple polypeptide hormone gene M dCEP7 of regulation and control root system development and its application |
| CN110063332A (en) * | 2019-05-21 | 2019-07-30 | 山东棉花研究中心 | It is a kind of improve root system of plant NO3--N translocation promotor and application |
| CN111620935A (en) * | 2019-02-27 | 2020-09-04 | 中国农业大学 | Application of ZmCEP1 gene in regulation and control of corn kernel development |
-
2021
- 2021-09-15 CN CN202111079997.0A patent/CN113754732B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245489A (en) * | 2017-06-08 | 2017-10-13 | 山东农业大学 | A kind of apple polypeptide hormone gene M dCEP7 of regulation and control root system development and its application |
| CN111620935A (en) * | 2019-02-27 | 2020-09-04 | 中国农业大学 | Application of ZmCEP1 gene in regulation and control of corn kernel development |
| CN110063332A (en) * | 2019-05-21 | 2019-07-30 | 山东棉花研究中心 | It is a kind of improve root system of plant NO3--N translocation promotor and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113754732A (en) | 2021-12-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113754732B (en) | Small molecular substance for improving cotton yield and fiber quality | |
| CN106866242A (en) | A kind of fertilizer synergist, enhanced fertilizer and preparation method thereof | |
| CN102285841B (en) | Fertilizer special for bamboo and production method thereof | |
| CN107306564B (en) | Method for diagnosing, regulating and fertilizing leaf age of rice in northern cold region | |
| CN101953249B (en) | Fertilization method for protected tomato overwinter long-season cultivation | |
| CN102584448A (en) | Compound fertilizer | |
| CN110698256A (en) | Water-soluble fertilizer containing fish protein | |
| CN112409058A (en) | Preparation method of amino acid-containing water-soluble fertilizer | |
| CN104108989A (en) | Kyoho grape special-purpose growth conditioning agent and application thereof | |
| CN108901507B (en) | Method for preventing and treating cotton verticillium wilt | |
| CN107556077B (en) | Special base fertilizer and additional fertilizer for grapes suitable for integration of water and fertilizer in southwest region | |
| CN109089782B (en) | Multi-fertilizer composition for vegetables and fertilization management method thereof | |
| CN114451165B (en) | Nitrogen synergist for regulating and controlling tobacco growth and use method and application thereof | |
| CN113754489A (en) | Multi-effect foliar fertilizer and preparation method thereof | |
| CN112174720A (en) | Joint-pulling fertilizer for water-fertilizer integrated planting of corn and preparation method and application thereof | |
| CN102381899A (en) | Compound organic fertilizer formula and application method | |
| CN110981588A (en) | Novel liquid rooting agent and preparation method thereof | |
| CN111253181A (en) | Drought-resistant foliar fertilizer | |
| CN112640756B (en) | Cultivation method for considering both shape forming and yield of mechanically harvested cotton plants in Hebei region | |
| CN107043299A (en) | A kind of ammonium nitrogen mill water culture nutrient solution in the corn time of infertility and preparation method thereof | |
| Doronin et al. | Sugar beet productivity formation depending on foliar application of microelements | |
| CN111777455A (en) | Lodging-resistant and yield-increasing silicon fertilizer for rice and preparation and use methods thereof | |
| CN111418324A (en) | Fertilizing method for cotton by replacing base fertilizer with additional fertilizer | |
| CN113087564A (en) | Controlled release fertilizer and preparation method and application thereof | |
| Georgieva et al. | Effect of bioorganic fertilizers and growth regulators on productivity and immune response of field tomatoes. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |