CN113712032A - Compound growth regulator and preparation method and application thereof - Google Patents
Compound growth regulator and preparation method and application thereof Download PDFInfo
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- CN113712032A CN113712032A CN202010465826.0A CN202010465826A CN113712032A CN 113712032 A CN113712032 A CN 113712032A CN 202010465826 A CN202010465826 A CN 202010465826A CN 113712032 A CN113712032 A CN 113712032A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Pest Control & Pesticides (AREA)
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- Agronomy & Crop Science (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a compound growth regulator and a preparation method and application thereof, and the compound growth regulator comprises an active ingredient and a synergistic assistant, wherein the active ingredient comprises the following components in parts by weight: 0.001-1 part of brassinolide and 1-10 parts of potassium hydroxy phosphate, wherein the synergistic additive comprises the following components in parts by weight: 0.1-5 parts of pollen polysaccharide. The compound growth regulator of the invention has the functions of promoting the healthy growth of crops, promoting the germination, rooting, stem leaf growth and flowering of crops, improving the fruit setting rate, enabling the crops to be early mature, enhancing the cold and drought resistance, enlarging fruits, increasing the yield and improving the fruit quality, does not generate phytotoxicity to the crops, is environment-friendly and highly safe to the crops.
Description
Technical Field
The invention relates to the technical field of agricultural growth regulators, in particular to a preparation method and application of a compound growth regulator containing brassinolide and potassium hydroxy phosphate.
Background
The plant growth regulator is a large class of chemical substances which have obvious effects on the growth and development of plants, are artificially extracted and synthesized according to the structure, function and action principle of plant hormones, and can change the synthesis, transportation, metabolism and effects of the hormones in plants so as to regulate the growth and development and physiological functions of the plants. It has been found that substances having a function of regulating plant growth and development include diethyl aminoethyl hexanoate (DA-6), forchlorfenuron, sodium nitrophenolate, auxin, gibberellin, ethylene, cytokinin, abscisic acid, brassinolide, salicylic acid, jasmonic acid, paclobutrazol, polyamine, and the like. The plant growth regulator can promote or inhibit the growth and development of plants at a lower concentration. Plant growth regulators have been used in crops, fruit trees and vegetables for a wide range of applications.
At present, with the continuous expansion of the application area of the plant growth regulator, the demand of plant growth regulation is also continuously increased, a plurality of plant growth regulators cannot achieve ideal effects when used alone, and the single application of the plant growth regulator has the following defects: the biological activity is single, the growth regulating effect is relatively low, the use amount (or use concentration) is large, and the growth regulating effect can be achieved only in a certain growth period of crops generally. When two or even more plant growth regulators are used in combination, the physiological effect of the combined action is often greater than the sum of the respective effects. Therefore, the compounding and blending preparation of the plant growth regulator has become one of the main development directions of the plant growth regulator industry.
Brassinolide, a novel green environment-friendly plant growth regulator, is treated by soaking seeds and spraying stems and leaves of brassinolide with proper concentration. Can promote the growth of vegetables, melons, fruits and other crops, improve the quality, improve the yield, have bright color and more thick leaves, and also can make melons and fruits have higher sugar content, larger individuals, higher yield and better storage resistance. Is harmless to human and livestock, and has safe and effective dosage when used normally. The natural brassin can be widely applied to various economic crops, can increase the yield by 5-10% and 20% higher, can obviously improve the quality, increase the sugar content and the weight of fruits and increase the gorgeous crops. Meanwhile, the drought resistance and the cold resistance of crops can be improved, and the symptoms of plant diseases and insect pests, phytotoxicity, fertilizer damage and freezing damage of the crops can be relieved.
Potassium salt of hydroxy phosphate of the formula (C)2H4O)nC12H26O·xH3O4P.xK, is nearA non-hormone, novel natural and environment-friendly crop nutrition conditioner is discovered in the years. The plant growth can be promoted through the absorption of stems and leaves of the plants, the accumulation of dry matters is increased, the permeability of cell membranes is improved, the content of chlorophyll is increased, and the photosynthetic strength is improved. Can make the sugar content of the melon and fruit higher, the fruit expands and the specific gravity of the fruit increases. It has the synergistic effect of auxin and cytokinin in controlling the number of branches and yield of plant. Can be widely applied to grain crops such as rice, wheat and potatoes, and can generally increase the yield by about 10 percent; the yield can be increased by 10-40% generally, can be increased by 5-15% generally on average, can reach a high yield of 30-40% and can obviously improve the quality when being applied to various economic crops such as fruit trees, vegetables, strawberries, melons and fruits, cotton and hemp, flowers and the like. Meanwhile, the drought resistance and the cold resistance of crops can be improved, and the symptoms of plant diseases and insect pests, phytotoxicity, fertilizer damage and freezing damage of the crops can be relieved.
The pollen polysaccharide is mainly polysaccharide active matter, and contains more than 20 kinds of amino acids, unsaturated fatty acid, phenols, vitamins, mineral elements, etc. The functions of the pollen polysaccharide on crops are mainly shown in that (1) root systems are promoted, the utilization rate of fertilizer is improved, and trees are strengthened; (2) the drought resistance and low temperature resistance of crops are improved, and the healthy growth of the crops is promoted; (3) the flower bud differentiation is promoted, the fruit is preserved, and the fruit swelling is realized, so that the crop yield is improved; (4) the fruit shape is improved, the fruit color of the fruit is improved, and the natural preservation and the shelf life of agricultural products are prolonged;
The prior art has no report about the preparation of a compound growth regulator by brassinolide, potassium hydroxy phosphate and pollen polysaccharide (synergist). Therefore, the reasonable and advanced formula not only is a reasonable distribution measure for blending plant nutrition and promoting plant growth and development, but also is a key method for ensuring the yield and quality of plants.
Disclosure of Invention
The invention aims to provide a compound plant growth regulator, and the technical scheme adopted by the invention is as follows in order to achieve the aim:
the compound growth regulator comprises an active ingredient and a synergistic auxiliary agent, wherein the active ingredient comprises the following components in parts by weight: 0.001-1 part of brassinolide and 1-20 parts of potassium hydroxy phosphate, wherein the synergistic additive comprises the following components in parts by weight: 0.1-2 parts of pollen polysaccharide and 0.1-5 parts of pollen polysaccharide.
Further, the brassinolide is selected from any one of 28-homobrassinolide, 24-epibrassinolide, propionyl brassinolide, 28-epibrassinolide, mixotrophin brassinolide or 14-hydroxy brassinosteroid.
Further, the pollen polysaccharide is extracted from cattail pollen, radix codonopsitis, corn, camellia, rape, pine, ginkgo and the like.
Further, the extraction step of the pollen polysaccharide comprises the following steps:
(1) preparation of a pollen sample: removing impurities from pollen group, pulverizing, extracting with petroleum ether (1: 4) in 70 deg.C water bath for 12 hr, and air drying.
(2) Crude extraction of pollen polysaccharides: alternately treating the pollen sample with-30 deg.C freezing and 70 deg.C heating repeatedly for more than 6 times, repeatedly performing water extraction, mixing extractive solutions, ethanol extraction, vacuum centrifuging, and drying at room temperature of 70 deg.C to obtain crude extract.
(3) Purifying and preparing pollen polysaccharide: and (3) removing protein from the crude extract by a trichloroacetic acid (TCA) method, repeatedly performing vacuum centrifugation, alcohol extraction, centrifugal precipitation and other processes, and finally performing Sephadex G-100 column chromatography purification to obtain a purified product.
Further, the method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture and an auxiliary agent allowed in pesticides into any one of a soluble solution, a soluble powder, an emulsifiable solution, a microemulsion, an emulsion in water, a suspending agent, a wettable powder or water dispersible granules and the like.
According to the technical scheme, the invention has the advantages that: promoting the healthy growth of crops, promoting the germination, rooting, stem leaf growth and flowering of crops, improving the fruit setting rate, enabling the crops to be early mature, enhancing the cold resistance and drought resistance, expanding fruits, increasing the yield and improving the fruit quality, generating no phytotoxicity on the crops, being environment-friendly and highly safe for the crops.
The invention also aims to protect the application of the compound growth regulator in planting fruits and vegetables such as strawberries or melons and vegetables.
Detailed Description
The following is a detailed description of specific embodiments of the invention.
Example 1
The compound growth regulator comprises an active ingredient and a synergistic auxiliary agent, wherein the active ingredient comprises the following components in parts by weight: 0.01 part of 28-high brassinolide and 10 parts of potassium hydroxy phosphate, wherein the synergistic additive comprises the following components in parts by weight: 1 part of pollen polysaccharide.
The extraction steps of the pollen polysaccharide comprise:
(1) preparation of a pollen sample: removing impurities from pollen group, pulverizing, extracting with petroleum ether (1: 4) in 70 deg.C water bath for 12 hr, and air drying.
(2) Crude extraction of pollen polysaccharides: alternately treating the pollen sample with-30 deg.C freezing and 70 deg.C heating repeatedly for more than 6 times, repeatedly performing water extraction, mixing extractive solutions, ethanol extraction, vacuum centrifuging, and drying at room temperature of 70 deg.C to obtain crude extract.
(3) Purifying and preparing pollen polysaccharide: purifying and preparing pollen polysaccharide: and (3) removing protein from the crude extract by a trichloroacetic acid (TCA) method, repeatedly performing vacuum centrifugation, alcohol extraction, centrifugal precipitation and other processes, and finally performing Sephadex G-100 column chromatography purification to obtain a purified product.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing missible oil with 13% of active ingredients in percentage by mass according to a conventional preparation method.
Example 2
The compound growth regulator comprises an active ingredient and a synergistic auxiliary agent, wherein the active ingredient comprises the following components in parts by weight: 0.15 part of 24-epibrassinolide and 20 parts of potassium hydroxy phosphate, wherein the synergistic additive comprises the following components in parts by weight: 2 parts of pollen polysaccharide.
The above pollen polysaccharides were extracted by the method described in example 1.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the microemulsion with the active ingredient mass percentage of 2.5% according to a conventional preparation method.
Example 3
The compound growth regulator comprises an active ingredient and a synergistic auxiliary agent, wherein the active ingredient comprises the following components in parts by weight: 0.075 part of 28-epibrassinolide and 13 parts of hydroxyl phosphate potassium salt, wherein the synergistic additive comprises the following components in parts by weight: 0.1 part of pollen polysaccharide.
The above pollen polysaccharides were extracted by the method described in example 1.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the mixture into the aqueous emulsion with the active ingredient mass percentage of 1.7% according to the conventional preparation method.
Example 4
The compound growth regulator comprises an active ingredient and a synergistic auxiliary agent, wherein the active ingredient comprises the following components in parts by weight: 1 part of mixed epibrassinolide and 1 part of potassium hydroxy phosphate, wherein the synergistic additive comprises the following components in parts by weight: 1 part of pollen polysaccharide.
The above pollen polysaccharides were extracted by the method described in example 1.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing the suspending agent with the active ingredient mass percentage of 0.75% according to a conventional preparation method.
Example 5
The compound growth regulator comprises an active ingredient and a synergistic auxiliary agent, wherein the active ingredient comprises the following components in parts by weight: propionyl brassinolide 0.007 parts, hydroxyl phosphate potassium salt 17 parts, the synergistic additive comprises the following components in parts by weight: 0.5 part of pollen polysaccharide.
The above pollen polysaccharides were extracted by the method described in example 1.
The method for preparing the compound growth regulator comprises the steps of uniformly mixing the raw materials in the weight ratio, and preparing wettable powder with the active ingredient mass percentage of 3.3% according to a conventional preparation method.
Example 6
The compound growth regulator comprises an active ingredient and a synergistic auxiliary agent, wherein the active ingredient comprises the following components in parts by weight: 0.75 part of 14-hydroxyl brassinol and 13 parts of hydroxyl phosphate potassium salt, wherein the synergistic additive comprises the following components in parts by weight: 5 parts of pollen polysaccharide.
The above pollen polysaccharides were extracted by the method described in example 1.
According to the method for preparing the compound growth regulator, the raw materials in the weight ratio are uniformly mixed, and the water dispersible granules with the active ingredient mass percentage of 4.0% are prepared according to a conventional preparation method.
Test of field drug effect
The efficacy test is divided into a test group and a control group, and the influence of the medicament provided by the invention on the growth of the strawberries is inspected. The strawberry variety is red cheek, and the grouping condition is as follows:
the test group agents are 1.3% of missible oil, 2.5% of microemulsion, 1.7% of aqueous emulsion, 0.75% of suspending agent, 3.3% of wettable powder and 4.0% of water dispersible granule prepared in examples 1-6, and can be listed as test 1, test 2, test 3, test 4, test 5 and test 6 in sequence.
The control components were control 1, control 2 and blank. Wherein, the drug of the comparison group 1 is 0.01 percent of mixotrophin lactone, and the comparison group 2 is mixotrophin lactone + potassium hydroxy phosphate.
Spraying the pesticide once respectively in the seedling stage, full-bloom stage and fruit expansion stage of the strawberries, and applying the pesticide three times in total. Spraying by using a Gongnong-16 knapsack manual sprayer every time, preparing a liquid medicine according to the medicine concentration of 3g.a.i/ha, and uniformly and thoroughly spraying the test strawberries.
For each treatment, 20 strawberries were randomly selected, and listed, and the 1 st flower of each treatment was recorded as the initial flowering stage, and the 1 st fruit was recorded as the fertile stage. The 1 st ripe fruit harvested was recorded as the primary harvest time and the last ripe fruit harvested as the last harvest time. Randomly weighing 20 fruits harvested per treatment, weighing 5 times in total, and calculating the average single fruit weight. Meanwhile, the mass of fruit harvested per treatment batch was recorded and the average individual yield was calculated. Randomly selecting 20 fruits from the fruits harvested in the full bearing period, and measuring the soluble solid content, Vc and soluble sugar content of the strawberries.
TABLE 1 Effect of different treatments on strawberry yield and quality
As can be seen from Table 1, the average single fruit weight, average single plant yield, soluble sugar content, VC content and soluble solid content of the strawberries of the drug treatments (test groups 1-6 and comparative groups 1-2) are higher than those of the blank control group.
In addition, indexes of the strawberries treated by the embodiment (test groups 1-6) of the invention are all higher than those of the strawberries treated by the comparative groups 1-2. The test result shows that the compound growth regulator provided by the invention has the advantages of obviously improving the growth, the yield, the fruit quality and the like of strawberries, generating no phytotoxicity to crops, being environment-friendly and highly safe to the crops.
The above embodiments are merely preferred embodiments of the present invention, and any simple modification, modification and substitution changes made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (7)
1. The compound growth regulator is characterized by comprising an active ingredient and a synergistic assistant, wherein the active ingredient comprises the following components in parts by weight: 0.001-1 part of brassinolide and 1-20 parts of potassium hydroxy phosphate, wherein the synergistic additive comprises the following components in parts by weight: 0.1-5 parts of pollen polysaccharide.
2. The complex growth regulator of claim 1, wherein the brassinolide is selected from the group consisting of 28-homobrassinolide, 24-epibrassinolide, propionyl brassinolide, 28-epibrassinolide, mixotrophin brassinolide and 14-hydroxybrassinosteroid.
3. The compound growth regulator of claim 1, wherein the pollen polysaccharides are extracted from pollen Typhae, radix Codonopsis, semen Maydis, flos Camelliae Japonicae, caulis et folium Brassicae campestris, pine Tree, semen Ginkgo, etc.
4. The compound growth regulator of claim 1, wherein the pollen polysaccharide extraction step comprises:
(1) preparation of a pollen sample: removing impurities from pollen group, pulverizing, extracting with petroleum ether (1: 4) in 70 deg.C water bath for 12 hr, and air drying.
(2) Crude extraction of pollen polysaccharides: alternately treating the pollen sample with-30 deg.C freezing and 70 deg.C heating repeatedly for more than 6 times, repeatedly performing water extraction, mixing extractive solutions, ethanol extraction, vacuum centrifuging, and drying at room temperature of 70 deg.C to obtain crude extract.
(3) Purifying and preparing pollen polysaccharide: purifying and preparing pollen polysaccharide: and (3) removing protein from the crude extract by a trichloroacetic acid (TCA) method, repeatedly performing vacuum centrifugation, alcohol extraction, centrifugal precipitation and other processes, and finally performing Sephadex G-100 column chromatography purification to obtain a purified product.
5. A method for preparing the compound growth regulator of claim 1, wherein the raw materials in the weight ratio are uniformly mixed and are prepared into any one of soluble solution, soluble powder, missible oil, microemulsion, emulsion in water, suspending agent, wettable powder or water dispersible granule with pesticide-allowable auxiliary agent.
6. The compound growth regulator according to claim 1, wherein the suspending agent comprises an oil suspending agent and an aqueous suspending agent.
7. The use of the compound growth regulator of claim 1 in planting fruits and vegetables such as strawberries or melons and vegetables.
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| CN202010465826.0A CN113712032A (en) | 2020-05-21 | 2020-05-21 | Compound growth regulator and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110637826A (en) * | 2019-10-21 | 2020-01-03 | 江西鑫邦生化有限公司 | Plant growth regulator and preparation method and application thereof |
| CN111937906A (en) * | 2020-08-31 | 2020-11-17 | 江西鑫邦科技有限责任公司 | Plant growth regulating mixture and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110637826A (en) * | 2019-10-21 | 2020-01-03 | 江西鑫邦生化有限公司 | Plant growth regulator and preparation method and application thereof |
| CN111937906A (en) * | 2020-08-31 | 2020-11-17 | 江西鑫邦科技有限责任公司 | Plant growth regulating mixture and preparation method and application thereof |
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Application publication date: 20211130 |