CN113661917A - Method for breeding granular indica-japonica intermediate type wide-compatibility restorer line - Google Patents
Method for breeding granular indica-japonica intermediate type wide-compatibility restorer line Download PDFInfo
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- CN113661917A CN113661917A CN202110727355.0A CN202110727355A CN113661917A CN 113661917 A CN113661917 A CN 113661917A CN 202110727355 A CN202110727355 A CN 202110727355A CN 113661917 A CN113661917 A CN 113661917A
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- japonica
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
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Abstract
The invention relates to a breeding method of a granular indica-japonica intermediate wide-affinity restorer line, which comprises the following steps: one of the conventional round-grained non-glutinous rice, the restorer or the maintainer is hybridized with the indica-japonica intermediate wide-affinity restorer, the first generation is self-bred, the grain type of each individual plant of the group is observed and separated, the individual plant with the round grain type and excellent plant leaf morphology is selected from the group to harvest seeds, the continuous multi-generation self-breeding is carried out, the plant line is taken as a unit, the grain type and the plant leaf morphology of each individual plant of each plant line are selected by each generation, and the genetically stable cluster indica-japonica intermediate wide-affinity restorer is obtained after the continuous multi-generation selection. The method has strong operability, does not need high-precision complex steps for cultivation, and has strong universality.
Description
Technical Field
The invention relates to a breeding method of a granular indica-japonica intermediate wide-affinity restorer line, belonging to the technical field of rice breeding.
Background
The utilization of heterosis greatly improves the yield per unit of rice in China, and the current planting area of hybrid rice accounts for about 50 percent of the total planting area of rice in China. The breeding of new hybrid rice varieties is the original power of the development of the hybrid rice industry, and the early hybrid rice varieties bred in China mainly comprise subspecies intraspecific hybrid rice and are mainly concentrated in subspecies of indica type; because the problems of affinity and the like of the interspecific hybrid rice are solved, and the great advantages in the aspects of yield, resistance and the like are also adopted, more and more new varieties of the indica-japonica interspecific hybrid rice are approved in recent years, the utilization level of the rice heterosis in China is further improved, the further development of the hybrid rice industry in China is promoted, a new breeding stage is also predicted to come, and the current stage is just in a transition stage, namely the stage of transition from the subspecies internal hybridization to the indica-japonica interspecific hybridization.
At present, a plurality of new indica-japonica hybrid rice varieties are independently or jointly bred in middle and lower reaches of Yangtze river, in particular in a plurality of breeding units and seed enterprises in Zhejiang province, wherein the new varieties are represented by Yongyou series varieties bred by Ningbo city agricultural academy and spring-you series varieties bred by Chinese rice institute, and are currently in the stage of popularization and application.
As the research of the indica-japonica subspecies hybrid rice is late, the prior indica-japonica subspecies hybrid rice is mainly matched in a japonica non-indica recovery mode, and most of the hybrid rice utilizes a three-line hybrid system, so that the varieties of the two-line hybrid indica-japonica subspecies hybrid rice are less. Most of restorers of indica-japonica subspecies hybrid rice are indica-japonica intermediate restorers with wide affinity bred by indica-japonica introgression hybridization, the grain types of the restorers are between the cluster type of typical japonica rice and the long grain type of typical indica rice, the length-width ratio is about 2.4, the cluster type restorers are few, and the production requirements of various grain types cannot be met.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the existing problems and defects, the invention aims to provide a method for breeding a granular indica-japonica intermediate wide-affinity restorer line, which makes up the shortage of granules in the prior art, and has simple steps and easy operation.
The technical scheme is as follows: in order to realize the purpose, the invention adopts the following technical scheme:
a method for breeding a granular indica-japonica intermediate wide-affinity restorer line comprises the following steps:
step 1: hybridizing one of the conventional round-grained japonica rice, the restorer or the maintainer with the indica-japonica intermediate wide-affinity restorer to obtain F1；
Step 2: f obtained in step 11Selfing the first generation, observing and recording the obtained grain types;
and step 3: and (3) selecting cluster grain types from the grain types obtained in the step (2), wherein the plant leaves are different from those of typical japonica rice or indica rice, and taking the plant leaf shapes with luxuriant growth, good color change at the mature period and relatively large spike types to obtain the clustered indica rice and japonica rice intermediate wide-affinity restorer line.
Further, selfing the granular indica-japonica intermediate wide-affinity restorer line obtained in the step 3, selecting a granular type, a luxuriant growing, a good color change in the mature period and a relatively large ear type in each generation, then selfing, continuously screening for multiple generations, and finally obtaining the genetically stable granular indica-japonica intermediate wide-affinity restorer line seed.
Further, the agglomerate type is a particle type having an aspect ratio of 1.5 to 2.3.
Further, the rice grain of the agglomerate type is 6mm long.
Further, in the step 1, the round-grained japonica rice restorer line is used as a male parent to be hybridized with the indica-japonica intermediate wide-affinity restorer line.
Has the advantages that: compared with the prior art, the invention has the following advantages: the method is a novel breeding method of the granular indica-japonica intermediate wide-affinity restorer line, can realize the preparation of the granular indica-japonica intermediate wide-affinity restorer line, is easy to operate and saves cost.
Detailed Description
The present invention is further illustrated by the following examples, which are intended to be purely exemplary and are not intended to limit the scope of the invention, as various equivalent modifications of the invention will occur to those skilled in the art upon reading the present disclosure and fall within the scope of the appended claims.
Examples
Taking the granular restorer C84 and indica-japonica intermediate wide-affinity restorer T7 as examples, F is obtained by hybridization1Then F is added1Selfing the first generation, observing and recording to obtain each grain type of the single plant. The cluster grain type is picked out from each grain type obtained in step 2 and the plant leaves are different from those of typical japonica or indica rice. Namely, a grain type having a rice grain length of 6mm and an aspect ratio of 1.5 to 2.3. And the required plant leaves have excellent morphology, namely, the plants grow luxuriantly, the mature period is good, the color change is good, the ear type is relatively large, and finally the indica-japonica intermediate type wide-affinity restoring line with the property of granules can be obtained.
However, the existing method can not obtain the restoring line which simultaneously meets the intermediate type and wide affinity of the granules and the indica-japonica rice, only one or two of the restoring lines can be met, the method is complicated, and the complicated steps have the risk of influencing the purities of various degrees due to the requirements of the conditions such as medicines or temperature and the like, and simultaneously have the problem of not really putting into practice.
The embodiment shows that the granular indica-japonica intermediate wide-affinity restorer line with excellent properties can be obtained by the method, the target is clear, the accuracy of the final granular indica-japonica intermediate wide-affinity restorer line is higher, and the operability and the adaptability are stronger compared with the existing granular rice breeding.
Claims (5)
1. A method for breeding a granular indica-japonica intermediate wide-affinity restorer line is characterized in that: the method comprises the following steps:
step 1: hybridizing one of the conventional round-grained japonica rice, the restorer or the maintainer with the indica-japonica intermediate wide-affinity restorer to obtain F1；
Step 2: f obtained in step 11Selfing the first generation, observing and recording the obtained grain types;
and step 3: and (3) selecting cluster grain types from the grain types obtained in the step (2), wherein the plant leaves are different from those of typical japonica rice or indica rice, and taking the plant leaf shapes with luxuriant growth, good color change at the mature period and relatively large spike types to obtain the clustered indica rice and japonica rice intermediate wide-affinity restorer line.
2. The method for breeding a clustered indica-japonica intermediate wide-affinity restorer line according to claim 1, wherein the method comprises the following steps: and (3) selfing the granular indica-japonica intermediate wide-affinity restorer line obtained in the step (3), selecting a granular type and a large-variety indica-japonica intermediate wide-affinity restorer line in each generation, selfing the granular type and the large-variety indica-japonica intermediate wide-affinity restorer line with good color change in the mature period and relatively large spike type, and continuously screening for multiple generations to obtain the genetically stable granular indica-japonica intermediate wide-affinity restorer line seed.
3. The method for breeding a clustered indica-japonica intermediate wide-affinity restorer line according to claim 1, wherein the method comprises the following steps: the agglomerate type is a particle type with an aspect ratio of 1.5-2.3.
4. The method for breeding a clustered indica-japonica intermediate wide-affinity restorer line according to claim 3, wherein the method comprises the following steps: the rice grain of the pellet type is 6mm long.
5. The method for breeding a clustered indica-japonica intermediate wide-affinity restorer line according to claim 1, wherein the method comprises the following steps: in the step 1, the round grain type japonica rice restorer line is used as a male parent to be hybridized with the indica rice intermediate type wide-affinity restorer line.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110727355.0A CN113661917B (en) | 2021-06-29 | 2021-06-29 | Method for breeding granular indica-japonica intermediate type wide-compatibility restorer line |
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| CN202110727355.0A CN113661917B (en) | 2021-06-29 | 2021-06-29 | Method for breeding granular indica-japonica intermediate type wide-compatibility restorer line |
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| CN113661917A true CN113661917A (en) | 2021-11-19 |
| CN113661917B CN113661917B (en) | 2022-05-06 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640701A (en) * | 2012-05-16 | 2012-08-22 | 中国水稻研究所 | Selecting and breeding method for long-grained hybrid japonica rice |
| CN108849485A (en) * | 2018-09-10 | 2018-11-23 | 贵州省水稻研究所 | A kind of selection of fragrant rice |
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2021
- 2021-06-29 CN CN202110727355.0A patent/CN113661917B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640701A (en) * | 2012-05-16 | 2012-08-22 | 中国水稻研究所 | Selecting and breeding method for long-grained hybrid japonica rice |
| CN108849485A (en) * | 2018-09-10 | 2018-11-23 | 贵州省水稻研究所 | A kind of selection of fragrant rice |
Non-Patent Citations (4)
| Title |
|---|
| 华宇峰等: "滇型晚粳稻不育系春江29A的选育及应用", 《杂交水稻》 * |
| 吴险峰，吴让祥，金宜祥，张爱国，李根明，徐乐斌，宁会琴，王开宏: "水稻亚种强优组合选育初报", 《安徽农业科学》 * |
| 吴险峰等: "水稻亚种间优势利用广亲和恢复系的选育研究", 《安徽农业科学》 * |
| 宋昕蔚等: "水稻籼粳亚种间杂种优势利用研究进展与展望", 《科学通报》 * |
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