CN113616585A - Whitening liquid containing placenta mesenchymal stem cells and preparation method thereof - Google Patents
Whitening liquid containing placenta mesenchymal stem cells and preparation method thereof Download PDFInfo
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- CN113616585A CN113616585A CN202110536942.1A CN202110536942A CN113616585A CN 113616585 A CN113616585 A CN 113616585A CN 202110536942 A CN202110536942 A CN 202110536942A CN 113616585 A CN113616585 A CN 113616585A
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 56
- 239000007788 liquid Substances 0.000 title claims abstract description 55
- 210000002826 placenta Anatomy 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000284 extract Substances 0.000 claims abstract description 51
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000012228 culture supernatant Substances 0.000 claims abstract description 21
- 102000008186 Collagen Human genes 0.000 claims abstract description 18
- 108010035532 Collagen Proteins 0.000 claims abstract description 18
- 229920001436 collagen Polymers 0.000 claims abstract description 18
- 241000220317 Rosa Species 0.000 claims abstract description 17
- 235000012907 honey Nutrition 0.000 claims abstract description 17
- 239000003906 humectant Substances 0.000 claims abstract description 17
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 15
- 238000004113 cell culture Methods 0.000 claims abstract description 13
- 229930003231 vitamin Natural products 0.000 claims abstract description 9
- 235000013343 vitamin Nutrition 0.000 claims abstract description 9
- 239000011782 vitamin Substances 0.000 claims abstract description 9
- 229940088594 vitamin Drugs 0.000 claims abstract description 9
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims description 24
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 20
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 20
- 238000010438 heat treatment Methods 0.000 claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 10
- 229930003268 Vitamin C Natural products 0.000 claims description 10
- 229930003427 Vitamin E Natural products 0.000 claims description 10
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 10
- 230000003169 placental effect Effects 0.000 claims description 10
- 235000019154 vitamin C Nutrition 0.000 claims description 10
- 239000011718 vitamin C Substances 0.000 claims description 10
- 235000019165 vitamin E Nutrition 0.000 claims description 10
- 229940046009 vitamin E Drugs 0.000 claims description 10
- 239000011709 vitamin E Substances 0.000 claims description 10
- SDJPNDMWNUPUFI-VIFPVBQESA-N (4-hydroxyphenyl) (2s)-5-oxopyrrolidine-2-carboxylate Chemical compound C1=CC(O)=CC=C1OC(=O)[C@H]1NC(=O)CC1 SDJPNDMWNUPUFI-VIFPVBQESA-N 0.000 claims description 9
- 244000183278 Nephelium litchi Species 0.000 claims description 9
- 229950000857 pidobenzone Drugs 0.000 claims description 9
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- 238000005119 centrifugation Methods 0.000 claims description 2
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- 230000000694 effects Effects 0.000 abstract description 29
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- 206010014970 Ephelides Diseases 0.000 abstract description 8
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 230000037303 wrinkles Effects 0.000 abstract description 7
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- 210000003491 skin Anatomy 0.000 description 23
- 230000000052 comparative effect Effects 0.000 description 13
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- 230000014759 maintenance of location Effects 0.000 description 4
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- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 2
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- 230000002045 lasting effect Effects 0.000 description 2
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- 201000004624 Dermatitis Diseases 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/368—Carboxylic acids; Salts or anhydrides thereof with carboxyl groups directly bound to carbon atoms of aromatic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The invention discloses a whitening liquid containing placenta mesenchymal stem cells and a preparation method thereof, wherein the whitening liquid comprises the following components: 20-25 parts of placenta mesenchymal stem cell culture supernatant, 2-3 parts of rose extract, 1-2 parts of honey extract, 3-4 parts of humectant, 1-1.5 parts of vitamin, 0.5-1 part of Pidobenzonone, 0.3-0.5 part of salicylic acid, 2-2.5 parts of collagen liquid and 1-2 parts of litchi seed extract. The invention has the beneficial effects that: the freckle removing cream is mild in freckle removing, can effectively remove pigmentation, chloasma, black specks and freckles to enable the skin to be tender, white and natural, is beneficial to improving the freckle removing and whitening effects, is beneficial to improving the effects of moistening, beautifying, protecting and beautifying the skin, is beneficial to improving the effects of preventing wrinkles and resisting oxidation, can effectively resist free radicals, reduces the volume of pigment cells, can achieve the whitening effect, can effectively repair skin damage and remove scars, and can effectively improve the effects of moisturizing, locking water and preventing sunburn.
Description
Technical Field
The invention relates to the field of whitening of placenta mesenchymal stem cells, in particular to a whitening lotion containing placenta mesenchymal stem cells and a preparation method thereof.
Background
The skin is an important barrier of the human body. After the facial skin is exposed outside for a long time, epidermal cells are easily oxidized and damaged by wind and sunshine, so that the skin becomes dry, and burns, skin inflammation and scars can be possibly caused. Furthermore, with age, the renewal of skin cells slows down, leading to a slowing down of skin aging and apoptotic cell renewal, collagen loss in the skin, pigmentation and freckles. People in daily life often maintain the facial skin by applying water, milk and whitening liquid after cleaning the face.
However, most of the whitening liquid used in the market at present is composed of a single moisturizing or freckle removing substance and the like, the freckle removing element is single, freckle removing efficiency is low, the moisturizing substance is single, moisturizing effect is poor, the problem of lack of water on the surface skin can be relieved only temporarily, the effect is not lasting, and skin damage and scars can not be repaired.
Therefore, the whitening liquid has the effects of moisturizing for a long time and repairing the injury.
Disclosure of Invention
Based on the above, the invention aims to provide the whitening liquid containing the placenta mesenchymal stem cells and the preparation method thereof, so as to solve the technical problems that most of the whitening liquids used in the current market are composed of single moisturizing or freckle removing substances and the like, the freckle removing elements are single, the freckle removing efficiency is low, the moisturizing substances are single, the moisturizing effect is poor, the lack of water on the surface skin can be relieved only temporarily, the effect is not lasting, the skin injury cannot be repaired, and scars can be removed.
In order to achieve the purpose, the invention provides the following technical scheme: a whitening liquid containing placenta mesenchymal stem cells and a preparation method thereof comprise: placenta mesenchymal stem cell culture supernatant, rose extract, honey extract, humectant, vitamins, Pidobenzone, salicylic acid, collagen liquid and litchi seed extract.
By adopting the technical scheme, the freckle removing is mild, can effectively remove pigmentation, chloasma, black specks and freckles to make the skin tender, white and natural, is beneficial to improving the freckle removing and whitening effect, is beneficial to improving the effects of moistening, beautifying, protecting and beautifying skin, is beneficial to improving the effects of preventing wrinkles and resisting oxidation, can effectively resist free radicals, reduces the volume of pigment cells, can achieve the whitening effect, and can effectively repair skin injury and remove scars.
The invention further provides the components of the whitening liquid containing the placenta mesenchymal stem cells by weight: 20-25 parts of placenta mesenchymal stem cell culture supernatant, 2-3 parts of rose extract, 1-2 parts of honey extract, 3-4 parts of humectant, 1-1.5 parts of vitamin, 0.5-1 part of Pidobenzonone, 0.3-0.5 part of salicylic acid, 2-2.5 parts of collagen liquid and 1-2 parts of litchi seed extract.
By adopting the technical scheme, the freckle removing is mild, can effectively remove pigmentation, chloasma, black specks and freckles to make the skin tender, white and natural, is beneficial to improving the freckle removing and whitening effect, is beneficial to improving the effects of moistening, beautifying, protecting and beautifying skin, is beneficial to improving the effects of preventing wrinkles and resisting oxidation, can effectively resist free radicals, reduces the volume of pigment cells, can achieve the whitening effect, and can effectively repair skin injury and remove scars.
The invention is further configured as a preparation method of the placenta mesenchymal stem cell culture supernatant, which comprises the following steps: culturing the placenta mesenchymal stem cells by a serum-free culture medium at the culture temperature of 35-37 ℃ for 72-96 h, centrifuging at the centrifugal rotation speed of 12000-13000 rpm for 20-30 min, and collecting supernatant after centrifugation to obtain the placenta mesenchymal stem cell culture supernatant.
By adopting the technical scheme, the skin injury can be effectively repaired and scars can be removed, and the repair efficiency of injured cells can be improved.
The invention further provides that the humectant comprises a sealing agent, a moisture absorbent, a hydrophilic matrix and a light-proofing agent, and the humectant comprises the following components in percentage by weight: 0.7 to 1 portion of sealing agent, 0.7 to 1 portion of moisture absorbent, 0.7 to 1 portion of hydrophilic matrix and 0.7 to 1 portion of light-proofing agent.
By adopting the technical scheme, the effects of wrinkle prevention and oxidation resistance are favorably improved, and the effects of moisturizing, water locking and sun protection can be effectively improved.
The invention further provides that the vitamins include vitamin C and vitamin E.
By adopting the technical scheme, the anti-oxidation effect can be effectively enhanced, free radicals can be resisted, the volume of pigment cells is reduced, and the whitening effect can be achieved.
The invention is further provided that the preparation method of the whitening liquid containing the placenta mesenchymal stem cells comprises the following steps:
a1, mixing Pidobenzone and salicylic acid, heating and stirring uniformly to obtain solution A;
a2, mixing vitamin C and vitamin E, stirring, adding collagen liquid, stirring, and heating to obtain solution B;
a3, mixing the rose extract, the honey extract and the lychee seed extract, heating to 40-50 ℃, and uniformly stirring to obtain solution C;
and A4, when the solution A, the solution B and the solution C are cooled to 20 ℃, pouring the solution A, the solution B and the solution C into a container, mixing and stirring, then adding the prepared culture supernatant of the placenta mesenchymal stem cells and a humectant, and homogenizing for 5-10 min to obtain the whitening liquid containing the placenta mesenchymal stem cells.
By adopting the technical scheme, the freckle removing is mild, can effectively remove pigmentation, chloasma, black specks and freckles to make the skin tender, white and natural, is beneficial to improving the freckle removing and whitening effect, is beneficial to improving the effects of moistening, beautifying, protecting and beautifying skin, is beneficial to improving the effects of preventing wrinkles and resisting oxidation, can effectively resist free radicals, reduces the volume of pigment cells, can achieve the whitening effect, and can effectively repair skin injury and remove scars.
In summary, the invention mainly has the following beneficial effects: the freckle removing cream is mild in freckle removing, can effectively remove pigmentation, chloasma, black specks and freckles to enable the skin to be tender, white and natural, is beneficial to improving the freckle removing and whitening effects, is beneficial to improving the effects of moistening, beautifying, protecting and beautifying the skin, is beneficial to improving the effects of preventing wrinkles and resisting oxidation, can effectively resist free radicals, reduces the volume of pigment cells, can achieve the whitening effect, can effectively repair skin damage and remove scars, and can effectively improve the effects of moisturizing, locking water and preventing sunburn.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention.
The following describes an embodiment of the present invention based on its overall structure.
The whitening liquid containing the placenta mesenchymal stem cells comprises culture supernatant of the placenta mesenchymal stem cells, rose extract, honey extract, humectant, vitamin, Pidobenzone, salicylic acid, collagen liquid and lychee seed extract, and comprises the following components in parts by weight: 20-25 parts of placenta mesenchymal stem cell culture supernatant, 2-3 parts of rose extract, 1-2 parts of honey extract, 3-4 parts of humectant, 1-1.5 parts of vitamin, 0.5-1 part of Pidobenzonone, 0.3-0.5 part of salicylic acid, 2-2.5 parts of collagen liquid and 1-2 parts of litchi seed extract.
Example 1
The weight components of the whitening liquid containing the placenta mesenchymal stem cells are as follows: 25 parts of placenta mesenchymal stem cell culture supernatant, 3 parts of rose extract, 2 parts of honey extract, 1 part of sealant, 1 part of hygroscopic agent, 1 part of hydrophilic matrix, 1 part of light-proofing agent, 0.75 part of vitamin C, 0.75 part of vitamin E, 1 parts of Pidobenone, 0.5 part of salicylic acid, 2.5 parts of collagen liquid and 2 parts of litchi seed extract.
Example 1 preparation of a whitening solution containing placental mesenchymal stem cells:
b1, mixing 1 part of Pidobenzone and 0.5 part of salicylic acid, heating and stirring uniformly to obtain solution A;
b2, mixing 0.75 part of vitamin C and 0.75 part of vitamin E, stirring uniformly, adding
2.5 parts of collagen liquid is stirred and heated to obtain liquid B;
b3, mixing 3 parts of rose extract, 2 parts of honey extract and 2 parts of lychee seed extract, heating to 40-50 ℃, and uniformly stirring to obtain solution C;
b4, 1 part of sealant, 1 part of hygroscopic agent, 1 part of hydrophilic matrix and 1 part of light-proofing agent, mixing the components in a container, heating the mixture to 30 ℃, and homogenizing the mixture for 5min to obtain 4 parts of humectant;
and B5, when the liquid A, the liquid B and the liquid C are cooled to 20 ℃, pouring the liquid A, the liquid B and the liquid C into a container, mixing and stirring, then adding 25 parts of prepared culture supernatant of the placenta mesenchymal stem cells and 4 parts of humectant, and homogenizing for 5-10 min to obtain the whitening liquid containing the placenta mesenchymal stem cells.
Has the advantages that: the freckle removing cream is mild, can effectively remove pigmentation, chloasma, black specks and freckles to make skin tender, white and natural, is beneficial to improving the freckle removing and whitening effects, is beneficial to improving the effects of moistening, beautifying, protecting skin and beautifying, is beneficial to improving the effects of preventing wrinkles and resisting oxidation, can effectively resist free radicals, reduces the volume of pigment cells, can achieve the whitening effect, can effectively repair skin injury and remove scars, and can effectively improve the effects of moisturizing, locking water and preventing sun.
Comparative example 1
The weight components of the whitening liquid containing the placenta mesenchymal stem cells are as follows: 25 parts of placenta mesenchymal stem cell culture supernatant, 3 parts of rose extract, 2 parts of honey extract, 0.75 part of vitamin C, 0.75 part of vitamin E, 1 parts of Pidobenzone, 0.5 part of salicylic acid, 2.5 parts of collagen liquid and 2 parts of litchi seed extract.
Comparative example 1 preparation of a whitening solution containing placental mesenchymal stem cells:
c1, mixing 1 part of Pidobenzone and 0.5 part of salicylic acid, heating and stirring uniformly to obtain solution A;
c2, mixing 0.75 part of vitamin C and 0.75 part of vitamin E, stirring, adding
2.5 parts of collagen liquid is stirred and heated to obtain liquid B;
c3, mixing 3 parts of rose extract, 2 parts of honey extract and 2 parts of lychee seed extract, heating to 40-50 ℃, and uniformly stirring to obtain solution C;
and C4, when the solution A, the solution B and the solution C are cooled to 20 ℃, pouring the solution A, the solution B and the solution C into a container, mixing and stirring, and then adding 25 parts of prepared culture supernatant of the placenta mesenchymal stem cells to homogenize for 5-10 min, thus obtaining the whitening liquid containing the placenta mesenchymal stem cells.
Advantages of comparative example 1: the freckle removing agent is mild in freckle removing, can effectively remove pigmentation, chloasma, black speck and freckle to make skin tender, white and natural, is beneficial to improving the effects of wrinkle prevention and oxidation resistance, can effectively resist free radicals, reduces the volume of pigment cells, can achieve the whitening effect, and can effectively repair skin injury and remove scars.
Disadvantage of comparative example 1: the moisture retention is poor, and the skin can not be locked, so that the moisture retention time is short, the sun protection effect is poor, and the skin can not be prevented from being damaged by external ultraviolet rays.
Comparative example 2
The weight components of the whitening liquid containing the placenta mesenchymal stem cells are as follows: 25 parts of placenta mesenchymal stem cell culture supernatant, 3 parts of rose extract, 2 parts of honey extract, 1 parts of Pidobenone, 0.5 part of salicylic acid, 2.5 parts of collagen liquid and 2 parts of litchi seed extract.
Comparative example 2 preparation of a whitening solution containing placental mesenchymal stem cells:
d1, mixing 1 part of Pidobenzone and 0.5 part of salicylic acid, heating and stirring uniformly to obtain solution A;
d2, mixing 3 parts of rose extract, 2 parts of honey extract and 2 parts of lychee seed extract, heating to 40-50 ℃, and uniformly stirring to obtain solution C;
d3, when the solution A and the solution C are cooled to 20 ℃, pouring the solution A and the solution C into a container for mixing and stirring, then adding 25 parts of prepared culture supernatant of the placenta mesenchymal stem cells and 2.5 parts of collagen liquid, and homogenizing for 5-10 min to obtain the whitening liquid containing the placenta mesenchymal stem cells.
Advantages of comparative example 2: the freckle is milder, can effectively remove pigmentation, chloasma, black speck and freckle, makes the skin tender, white and natural, and can effectively repair skin injury and remove scars.
Disadvantage of comparative example 2: the moisture retention is poor, and the skin can not be locked, so that the moisture retention time is short, the sun protection effect is poor, the skin can not be prevented from being damaged by external ultraviolet rays, and the effects of wrinkle prevention and oxidation resistance are poor.
Comparative example 3
The weight components of the whitening liquid containing the placenta mesenchymal stem cells are as follows: 25 parts of placenta mesenchymal stem cell culture supernatant, 3 parts of rose extract, 2 parts of honey extract, 1 part of sealant, 1 part of hygroscopic agent, 1 part of hydrophilic matrix, 1 part of light-proofing agent, 0.75 part of vitamin C, 0.75 part of vitamin E, 2.5 parts of collagen liquid and 2 parts of litchi seed extract.
Comparative example 3 preparation of a whitening solution containing placental mesenchymal stem cells:
e1, mixing 0.75 part of vitamin C and 0.75 part of vitamin E, stirring uniformly, adding
2.5 parts of collagen liquid is stirred and heated to obtain liquid B;
e2, mixing 3 parts of rose extract, 2 parts of honey extract and 2 parts of lychee seed extract, heating to 40-50 ℃, and uniformly stirring to obtain solution C;
e3, 1 part of sealant, 1 part of moisture absorbent, 1 part of hydrophilic matrix and 1 part of light-proofing agent, mixing the components in a container, heating to 30 ℃, and homogenizing for 5min to obtain 4 parts of humectant;
e4, when the liquid B and the liquid C are cooled to 20 ℃, pouring the liquid B and the liquid C into a container, mixing and stirring, then adding 25 parts of prepared culture supernatant of the placenta mesenchymal stem cells and 4 parts of humectant, and homogenizing for 5-10 min to obtain the whitening liquid containing the placenta mesenchymal stem cells.
Advantages of comparative example 3: the whitening and skin-protecting mask is beneficial to improving the effects of moistening, beautifying, protecting and beautifying, improving the effects of preventing wrinkles and resisting oxidation, effectively resisting free radicals, reducing the volume of pigment cells, further achieving the whitening effect, effectively repairing skin injury, and effectively improving the effects of moisturizing, locking water and preventing sun.
Disadvantage of comparative example 3: the freckle removing effect is poor, pigmentation, chloasma, black specks and freckles cannot be effectively removed, and the whitening effect is poor.
The comparison of the examples with the comparative examples shows that the product of example 1 produces better results.
Although embodiments of the present invention have been shown and described, it is intended that the present invention should not be limited thereto, that the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples, and that modifications, substitutions, variations or the like, which are not inventive and may be made by those skilled in the art without departing from the principle and spirit of the present invention and without departing from the scope of the claims.
Claims (7)
1. A whitening lotion containing placenta mesenchymal stem cells and a preparation method thereof are characterized by comprising the following steps: placenta mesenchymal stem cell culture supernatant, rose extract, honey extract, humectant, vitamins, Pidobenzone, salicylic acid, collagen liquid and litchi seed extract.
2. The whitening solution containing placental mesenchymal stem cells according to claim 1 and the preparation method thereof, wherein the whitening solution comprises: the whitening liquid containing the placenta mesenchymal stem cells comprises the following components in parts by weight:
20-25 parts of placenta mesenchymal stem cell culture supernatant, 2-3 parts of rose extract, 1-2 parts of honey extract, 3-4 parts of humectant, 1-1.5 parts of vitamin, 0.5-1 part of Pidobenzonone, 0.3-0.5 part of salicylic acid, 2-2.5 parts of collagen liquid and 1-2 parts of litchi seed extract.
3. The whitening solution containing placental mesenchymal stem cells according to claim 1 and the preparation method thereof, wherein the whitening solution comprises: the preparation method of the culture supernatant of the placenta mesenchymal stem cells comprises the following steps: culturing the placenta mesenchymal stem cells by a serum-free culture medium at the culture temperature of 35-37 ℃ for 72-96 h, centrifuging at the centrifugal rotation speed of 12000-13000 rpm for 20-30 min, and collecting supernatant after centrifugation to obtain the placenta mesenchymal stem cell culture supernatant.
4. The whitening solution containing placental mesenchymal stem cells according to claim 1 and the preparation method thereof, wherein the whitening solution comprises: the humectant comprises a sealing agent, a moisture absorbent, a hydrophilic matrix and a light-proofing agent.
5. The whitening solution containing placental mesenchymal stem cells according to claim 1 and the preparation method thereof, wherein the whitening solution comprises: the vitamins include vitamin C and vitamin E.
6. The whitening solution containing placental mesenchymal stem cells according to claim 1 and the preparation method thereof, wherein the whitening solution comprises: the humectant comprises the following components in percentage by weight:
0.7 to 1 portion of sealing agent, 0.7 to 1 portion of moisture absorbent, 0.7 to 1 portion of hydrophilic matrix and 0.7 to 1 portion of light-proofing agent.
7. The whitening solution containing placental mesenchymal stem cells according to claim 1 and the preparation method thereof, wherein the whitening solution comprises: the preparation method of the whitening liquid containing the placenta mesenchymal stem cells comprises the following steps:
a1, mixing Pidobenzone and salicylic acid, heating and stirring uniformly to obtain solution A;
a2, mixing vitamin C and vitamin E, stirring, adding collagen liquid, stirring, and heating to obtain solution B;
a3, mixing the rose extract, the honey extract and the lychee seed extract, heating to 40-50 ℃, and uniformly stirring to obtain solution C;
and A4, when the solution A, the solution B and the solution C are cooled to 20 ℃, pouring the solution A, the solution B and the solution C into a container, mixing and stirring, then adding the prepared culture supernatant of the placenta mesenchymal stem cells and a humectant, and homogenizing for 5-10 min to obtain the whitening liquid containing the placenta mesenchymal stem cells.
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