CN113599349A

CN113599349A - Traditional Chinese medicine tooth-cleaning gel for dogs and preparation method thereof

Info

Publication number: CN113599349A
Application number: CN202111087485.9A
Authority: CN
Inventors: 李静; 阮崇美; 胡倩倩; 熊永洁; 赵晋颐; 贺绍君; 刘德义
Original assignee: Anhui University of Science and Technology
Current assignee: Anhui University of Science and Technology
Priority date: 2021-09-16
Filing date: 2021-09-16
Publication date: 2021-11-05
Anticipated expiration: 2041-09-16
Also published as: CN113599349B

Abstract

The invention provides a traditional Chinese medicine tooth-cleaning gel for dogs and a preparation method thereof, wherein the traditional Chinese medicine tooth-cleaning gel for dogs is a medicinal powder tooth-cleaning gel or a medicinal liquid tooth-cleaning gel; can be used for treating canine dental calculus, dental plaque, gingival inflammation, and oral odor. The preparation method of the medicinal powder tooth-cleaning gel comprises the following steps: s101, taking radix sophorae flavescentis, dried alum, rhizoma drynariae and mint, crushing and sieving, adding pseudo-ginseng powder, lactic acid bacteria and tea polyphenol, and fully mixing; s102, heating and dissolving carbomer and water, cooling to room temperature, and adjusting the pH value to 5.5-6.5; s103, fully mixing the Chinese herbal compound powder of S101 with the carbomer gel of S102 to obtain the Chinese herbal compound powder; the preparation method of the liquid medicine tooth-cleaning gel comprises the following steps: s201, soaking radix sophorae flavescentis and rhizoma drynariae in water for 2 hours, decocting for 1.5-2 hours, adding pseudo-ginseng powder and dried alum, continuously decocting for 1-2 hours, adding mint, stopping heating, and filtering; s202, adding carbomer into the Chinese medicine liquid in the S201, heating to dissolve, cooling to room temperature, adding lactic acid bacteria and tea polyphenol, and adjusting the pH to 5.5-6.5 to obtain the oral liquid.

Description

Traditional Chinese medicine tooth-cleaning gel for dogs and preparation method thereof

Technical Field

The invention relates to the technical field of oral care products, in particular to a traditional Chinese medicine tooth-cleaning gel for dogs and a preparation method thereof.

Background

Canine tooth disease has a high incidence and is clinically common as a syndrome: calculus, dental plaque, gingival inflammation, oral odor, etc. If not prevented or treated in time, it can cause chewing difficulties, oronasal fistulas, alveolar abscesses, and even mandibular solubility fractures. Therefore, the pet health product market is in great demand.

At present, the canine tooth related gel in the market is mainly used for scraping dental calculus and dental plaque through curdling, has no obvious effect and does not have the treatment effects of fixing teeth and treating gingival inflammation. There is also no study of the results of the bacteriostatic effect. At present, no related traditional Chinese medicine compound tooth gel product exists in the market, and the traditional Chinese medicine compound tooth gel product is basically western medicines or other plant extract components.

Disclosure of Invention

In order to solve the problems, the invention aims to provide a traditional Chinese medicine tooth-cleaning gel for dogs and a preparation method thereof.

In order to achieve the above object, the technical solution of the present invention is as follows.

A preparation method of traditional Chinese medicine tooth-cleaning gel for dogs is provided, wherein the traditional Chinese medicine tooth-cleaning gel for dogs is medicinal powder tooth-cleaning gel or medicinal liquid tooth-cleaning gel prepared from radix Sophorae Flavescentis, dried Alumen, rhizoma Drynariae, herba Menthae, Notoginseng radix powder, lactobacillus, tea polyphenols, and carbomer;

the preparation method of the medicinal powder tooth-cleaning gel comprises the following steps:

s101, crushing and sieving radix sophorae flavescentis, dried alum, rhizoma drynariae and mint, adding pseudo-ginseng powder, lactic acid bacteria and tea polyphenol, and fully mixing to obtain traditional Chinese medicine compound powder;

s102, fully mixing carbomer with water, heating to dissolve the carbomer, cooling to room temperature, and adjusting the pH value to 5.5-6.5 to obtain carbomer gel;

s103, fully mixing the Chinese herbal compound powder prepared in the S101 with the carbomer gel prepared in the S102 to obtain medicinal powder tooth-cleaning gel;

the preparation method of the liquid medicine tooth-cleaning gel comprises the following steps:

s201, soaking radix sophorae flavescentis and rhizoma drynariae in water for 2 hours, decocting for 1.5-2 hours, adding pseudo-ginseng powder and dried alum, continuously decocting for 1-2 hours, adding mint, stopping heating, and filtering to obtain a traditional Chinese medicine liquid;

s202, adding carbomer into the traditional Chinese medicine liquid obtained in the step S201, heating to dissolve the carbomer, cooling to room temperature, adding lactic acid bacteria and tea polyphenol, fully mixing, and adjusting the pH to 5.5-6.5 to obtain the liquid medicine tooth-cleaning gel.

It should be noted that the fire was turned off immediately after 10min of mint addition to stop heating, mainly because the mint was not decocted for a long time.

Further, the mass ratio of the radix sophorae flavescentis, the dried alum, the rhizoma drynariae, the mint and the pseudo-ginseng powder is 10-30: 1-3: 2-6: 1-3: 2-6.

Further, in S102, the ratio of carbomer to water is 20 g: 150 and 200 mL.

Further, in S103, the mass ratio of the traditional Chinese medicine compound powder to the carbomer gel is 40: 180-220.

Further, in S202, the dosage ratio of the traditional Chinese medicine liquid to the carbomer is 150-: 20 g.

Further, triethanolamine was used for pH adjustment in both S102 and S202.

Further, the mass ratio of the lactic acid bacteria to the tea polyphenol is 2-4: 1-2.

Furthermore, in the preparation step of the traditional Chinese medicine tooth-cleaning gel for dogs, after lactic acid bacteria and tea polyphenol are added, pyrophosphate and sorbitol are also added; wherein the mass ratio of the lactic acid bacteria to the tea polyphenol to the pyrophosphate to the sorbitol is 2-4: 1-2: 40-60: 4-8.

Further, the carbomer is carbomer 943 p.

The invention also provides the traditional Chinese medicine tooth-cleaning gel for dogs, which is prepared according to the method.

The invention has the beneficial effects that:

1. the invention mainly adopts two preparation processes to respectively prepare the medicinal powder tooth-cleaning gel and the medicinal liquid tooth-cleaning gel. Compared with the two processes, the preparation process is simple and consumes less time. The experiments show that the medicinal liquid tooth-cleaning gel has more remarkable treatment effect on canine dental calculus and dental plaque than the medicinal powder tooth-cleaning gel. The effect of the Chinese herbal medicines after decoction is generally better than that of the medicines directly ground and pulverized. However, the time for the dog drug solution gel to adhere is not longer than that of the dog drug powder gel, and the proportion of the carbomer 943P to the drug solution in the preparation of the drug solution gel can be continuously discussed and improved.

2. The traditional Chinese medicine tooth-cleaning gel for dogs prepared by the two preparation processes has curative effects on canine dental calculus, dental plaque, gingival inflammation and oral odor. But the liquid medicine tooth-cleaning gel has better treatment effect on dental calculus and dental plaque.

Drawings

FIG. 1 is a photograph of teeth taken with 0d and 17d of the medicinal powder tooth cleaning gel of example 3 of the present invention. Wherein (a) is a 0d dental photograph of the medication; (b) the photograph of the teeth is taken for 17 d.

FIG. 2 is a photograph of teeth on liquid dentifrice gel of example 4 of the present invention taken at 0d and 17 d. Wherein (a) is a 0d dental photograph of the medication; (b) the photograph of the teeth is taken for 17 d.

FIG. 3 is a VITA16 color panel.

FIG. 4 is a histogram comparing the canine tooth color number before and after gel administration of the group A powder.

FIG. 5 is a bar graph comparing the canine tooth color number before and after gel administration of the group B solutions.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Experimental animals: 12 Chinese rural dogs under the age of 2 years old.

Experimental drugs: sorbitol (Shijiazhuang Ruixue pharmaceutical Co., Ltd.), triethanolamine (import 20190501, Toxico Yatai Co., Ltd.), mixed feed additive lactobacillus acidophilus (2014029042, Shandong kang dien Biotech Co., Ltd.), carbomer 943P (20190418, Fujian Borui Biotech Co., Ltd.), tea polyphenol (20181209, Jiangxi Yuanfu Yuan Biotech Co., Ltd.), sodium pyrophosphate (20190312, Hubei Xingfu chemical group Ltd.), pulvis radix Notoginseng, rhizoma Drynariae, herba Menthae, and dried Alumen.

Experimental article and apparatus: decocting pot, 200 mesh sieve, gauze, culture dish, LB nutrient agar (Qingdao high-tech industrial garden Haibo Biotechnology Co., Ltd.), water bath heating pot, glass rod, agar strip, inoculating loop, high-pressure steam sterilizing pot, incubator, centrifuge, pH meter, and refrigerator at 4 deg.C.

The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.

Example 1

The traditional Chinese medicine tooth-cleaning gel for dogs is medicinal powder tooth-cleaning gel, and the preparation method of the medicinal powder tooth-cleaning gel is as follows:

s1, putting 20g of radix sophorae flavescentis, 2g of dried alum, 4g of rhizoma drynariae and 2g of mint into a traditional Chinese medicine grinder for grinding and grinding, filtering by using a 200-mesh sieve, adding 4g of pseudo-ginseng powder, 2g of lactic acid bacteria and 1g of tea polyphenol, and fully mixing to obtain traditional Chinese medicine compound powder;

s2, weighing 20g of carbomer 943p, fully mixing with 180mL of distilled water, heating in a water bath to fully dissolve the carbomer 943p and the distilled water, cooling to room temperature, gradually dropwise adding triethanolamine, fully stirring, and measuring the pH value by using a pH titrator for multiple times until the pH value is about 6.0 to obtain carbomer gel;

s3, fully mixing 40g of the Chinese herbal compound powder prepared in the S1 and 200g of carbomer gel prepared in the S2 to obtain the medicinal powder tooth-cleaning gel.

Example 2

The traditional Chinese medicine tooth-cleaning gel for dogs is a liquid medicine tooth-cleaning gel, and the preparation method of the liquid medicine tooth-cleaning gel is as follows:

s1, adding 100g of radix sophorae flavescentis and 20g of rhizoma drynariae into 1500mL of water for soaking for 2h, pouring out 500mL of soaking solution for later use, boiling the materials with strong fire, decocting the materials with slow fire for 1.5h, wherein the total decocting time is 2h, pouring 500mL of soaking solution, adding 20g of pseudo-ginseng powder and 10g of dried alum, continuing to decoct the materials for 1.5h, adding 20g of mint 10min before turning off the fire, stopping heating, filtering the materials with gauze, repeatedly filtering filter residues with water for 3 times, and combining the filtrates to obtain a traditional Chinese medicine liquid;

s2, taking 180mL of the S1 traditional Chinese medicine liquid, adding 20g of carbomer, heating to dissolve, cooling to room temperature, adding 2g of lactic acid bacteria and 1g of tea polyphenol, fully mixing, dropwise adding triethanolamine, and adjusting the pH to 6.0 to obtain the liquid medicine tooth-cleaning gel.

Example 3

s1, putting 20g of radix sophorae flavescentis, 2g of dried alum, 4g of rhizoma drynariae and 2g of mint into a traditional Chinese medicine grinder for grinding and grinding, filtering by using a 200-mesh sieve, adding 4g of pseudo-ginseng powder, 2g of lactic acid bacteria, 1g of tea polyphenol, 48g of sodium pyrophosphate and 4g of sorbitol, and fully mixing to obtain traditional Chinese medicine compound powder;

Example 4

s1, adding 100g of radix sophorae flavescentis and 20g of rhizoma drynariae into 1500mL of water for soaking for 2h, pouring out 500mL of soaking solution for later use, boiling the materials with strong fire, decocting the materials with slow fire for 1.5h, wherein the total decocting time is about 2h, pouring 500mL of soaking solution, adding 20g of pseudo-ginseng powder and 10g of dried alum, continuing to decoct the materials for 1.5h, adding 20g of mint 10min before turning off the fire, stopping heating, filtering the materials with gauze, repeatedly filtering filter residues with water for 1 time, and combining the filtrates to obtain a traditional Chinese medicine liquid;

s2, taking 180mL of the S1 traditional Chinese medicine liquid, adding 20g of carbomer, heating to dissolve, cooling to room temperature, adding 2g of lactic acid bacteria, 1g of tea polyphenol, 48g of sodium pyrophosphate and 4g of sorbitol, fully mixing, dropwise adding triethanolamine, and adjusting the pH to 6.0 to obtain the liquid medicine tooth-cleaning gel.

Example 5

s1, putting 10g of radix sophorae flavescentis, 1g of dried alum, 2g of rhizoma drynariae and 1g of mint into a traditional Chinese medicine grinder for grinding and grinding, filtering by using a 200-mesh sieve, adding 2g of pseudo-ginseng powder, 3g of lactic acid bacteria and 1g of tea polyphenol, and fully mixing to obtain traditional Chinese medicine compound powder;

s2, weighing 20g of carbomer 943p, fully mixing with 150mL of distilled water, heating in a water bath to fully dissolve the carbomer 943p and the distilled water, cooling to room temperature, gradually dropwise adding triethanolamine, fully stirring, and measuring the pH value by using a pH titrator for multiple times until the pH value is about 5.5 to obtain carbomer gel;

s3, fully mixing 40g of the Chinese herbal compound powder prepared in the S1 and 180g of carbomer gel prepared in the S2 to obtain the medicinal powder tooth-cleaning gel.

Example 6

s1, putting 30g of radix sophorae flavescentis, 3g of dried alum, 6g of rhizoma drynariae and 3g of mint into a traditional Chinese medicine grinder for grinding and grinding, filtering by using a 200-mesh sieve, adding 6g of pseudo-ginseng powder, 4g of lactic acid bacteria and 2g of tea polyphenol, and fully mixing to obtain traditional Chinese medicine compound powder;

s2, weighing 20g of carbomer 943p, fully mixing with 200mL of distilled water, heating in a water bath to fully dissolve the carbomer 943p and the distilled water, cooling to room temperature, gradually dropwise adding triethanolamine, fully stirring, and measuring the pH value by using a pH titrator for multiple times until the pH value is about 6.5 to obtain carbomer gel;

s3, fully mixing 40g of the Chinese herbal compound powder prepared in the S1 and 220g of carbomer gel prepared in the S2 to obtain the medicinal powder tooth-cleaning gel.

Example 7

s1, adding 100g of radix sophorae flavescentis and 30g of rhizoma drynariae into 1500mL of water for soaking for 2h, pouring out 500mL of soaking solution for later use, boiling the materials with strong fire, decocting the materials with slow fire for 1.5h, wherein the total decocting time is 2h, pouring 500mL of soaking solution, adding 30g of pseudo-ginseng powder and 10g of dried alum, continuing to decoct the materials for 1.5h, adding 20g of mint 10min before turning off the fire, stopping heating, filtering the materials with gauze, repeatedly filtering filter residues with water for 3 times, and combining the filtrates to obtain a traditional Chinese medicine liquid;

s2, taking 150mL of S1 traditional Chinese medicine liquid, adding 20g of carbomer, heating to dissolve, cooling to room temperature, adding 2g of lactic acid bacteria and 1g of tea polyphenol, fully mixing, dropwise adding triethanolamine, and adjusting pH to 5.5 to obtain the liquid medicine tooth-cleaning gel.

Example 8

s2, taking 200mL of the S1 traditional Chinese medicine liquid, adding 20g of carbomer, heating to dissolve, cooling to room temperature, adding 2g of lactic acid bacteria and 1g of tea polyphenol, fully mixing, dropwise adding triethanolamine, and adjusting the pH to 6.5 to obtain the liquid medicine tooth-cleaning gel.

Example 9

s1, putting 20g of radix sophorae flavescentis, 2g of dried alum, 4g of rhizoma drynariae and 2g of mint into a traditional Chinese medicine grinder for grinding and grinding, filtering by using a 200-mesh sieve, adding 4g of pseudo-ginseng powder, 2g of lactic acid bacteria, 1g of tea polyphenol, 40g of sodium pyrophosphate and 5g of sorbitol, and fully mixing to obtain traditional Chinese medicine compound powder;

Example 10

s2, taking 180mL of the S1 traditional Chinese medicine liquid, adding 20g of carbomer, heating to dissolve, cooling to room temperature, adding 2g of lactic acid bacteria, 1g of tea polyphenol, 60g of sodium pyrophosphate and 8g of sorbitol, fully mixing, dropwise adding triethanolamine, and adjusting the pH to 6.0 to obtain the liquid medicine tooth-cleaning gel.

The prepared traditional Chinese medicine tooth-cleaning gel is stored in a refrigerator at the temperature of 4 ℃ and is stored in the shade as far as possible, and the carbomer gel is easily dissolved and the viscosity is reduced due to overhigh temperature. In order to improve the stability of the traditional Chinese medicine tooth cleaning gel, 0.5 wt% of sodium benzoate can be added into the gel product obtained in the above example 1-10.

The performances of the gel products obtained in examples 1 to 10 were substantially the same, and only the performances of the medicinal gel for cleaning teeth obtained in example 3 and the medicinal gel for cleaning teeth obtained in example 4 were compared.

First, stability detection

1. The medicinal powder tooth-cleaning gel prepared in example 3 and the medicinal liquid tooth-cleaning gel prepared in example 4 are respectively subpackaged in a centrifuge tube, and whether the gel stability is changed or not is observed after a centrifuge is used for 30min at 3000 r/min.

2. The powder dentifrice gel prepared in example 3 and the liquid dentifrice gel prepared in example 4 were stored in a refrigerator at 4 deg.C for 14 days, and the gel stability was observed.

3. Results of the experiment

From the stability test, it is found that the medicinal tooth cleaning gel prepared in example 3 and the medicinal tooth cleaning gel prepared in example 4 are both excellent in stability. After being centrifuged for 30min at 3000r/min by the centrifuge, the states of the medicinal powder tooth-cleaning gel prepared in the example 3 and the medicinal liquid tooth-cleaning gel prepared in the example 4 are not changed. After being placed in a refrigerator at 4 ℃ for 14 days, the stability of the medicinal powder tooth cleaning gel prepared in the example 3 and the medicinal liquid tooth cleaning gel prepared in the example 4 is not changed. However, after the medicinal powder tooth cleaning gel prepared in example 3 is coated on the surfaces of dogs, the medicinal powder tooth cleaning gel prepared in example 3 has longer adhesion time and stronger adhesion force compared with the medicinal powder tooth cleaning gel prepared in example 4.

Second, effect detection

1. Laboratory animal

12 Chinese countryside dogs under 2 years old have good physical condition.

During the test period, the dogs are fed with the natural nutritional dietary formula dog food (nutritional ingredients: crude fiber is less than or equal to 4.5%, crude protein is more than or equal to 28%, crude fat is more than or equal to 16%, calcium is more than or equal to 1.1%, crude ash is less than or equal to 9%, phosphorus is more than or equal to 0.9%, linoleic acid is more than or equal to 1.4%, and water is less than or equal to 10%) at eight morning and evening every day (baoding pet food manufacturing limited company). Except fasting and water prohibition within 2h after administration, water is freely drunk in the rest time.

Before administration, the cotton swab should be used to wipe food residue and saliva on canine teeth surface, and then the cotton swab is used to smear gel medicine, so as to increase adhesion time. In 2h after the use, dogs should be fasted and forbidden to drink, so that the traditional Chinese medicine tooth-cleaning gel attached to the surfaces of teeth is prevented from being scraped off due to the biting of food of the dogs. When in use, the repeated use of the cotton swab is avoided, and the canine oral bacteria can be brought into the canine traditional Chinese medicine compound gel, so that the decay and deterioration of the traditional Chinese medicine compound gel are accelerated, and the storage is not facilitated.

The traditional Chinese medicine tooth cleaning gel for dogs is stored in a refrigerator at 4 ℃ and is protected from light.

2. Design of experiments

The test is divided into two groups: group A and group B. A total of 12 Zhonghua garden dogs were selected, and the 12 dogs were divided into A, B groups of 6 dogs each.

Before administration, the 12 canine teeth to 4 th molar teeth of canine teeth with dental calculus and dental plaque were subjected to data measurement. 19 representative dental calculus and dental plaque were selected for each of group A and group B. And collecting canine dental calculus and bacteria on dental plaque surface, and separating and culturing.

Group A was administered the pharmaceutical dentifrice gel prepared in example 3 and group B was administered the pharmaceutical dentifrice gel prepared in example 4.

The traditional Chinese medicine tooth cleaning gels prepared in the example 3 and the example 4 are respectively smeared on the surfaces of teeth of 12 test dogs suffering from dental calculus and dental plaque. Canine calculus, length and width of dental plaque, and photograph acquisition were performed on canine teeth with dental calculus, dental plaque between canine teeth to 4 th molar teeth of each dog at the dose of 0 d. The 2 nd calculus, plaque length, width measurements and photograph acquisition were performed after 7 days of continuous dosing. Measurements of calculus, plaque length, width and photograph acquisition were performed 3 times after 17 days of continuous dosing.

3. Detection method

3.1, visual measurement: the plaque and calculus size of the test dog is detected 4 days before the drug test of the test dog. After the medicine is continuously smeared for 7 days, the sizes of dental calculus and dental plaque are detected for the 2 nd time, and compared and analyzed with the 1 st detection result. After the medicine is continuously smeared for 17 days, the detection of the sizes of the dental calculus and the dental plaque is carried out for the 3 rd time, and the detection is compared and analyzed with the data measured in the previous two times.

3.2, counting the number of teeth with dental calculus and dental plaque according to the VITA16 color matching board shown in figure 3. The pre-dose and post-dose amounts were compared.

3.3, drug sensitivity test: before the test dog is administrated, bacteria on the surfaces of dental calculus and dental plaque of the test dog are scraped, an LB agar culture medium is adopted in a water-proof constant temperature box at 37 ℃, after 24 hours of culture, partial bacterial colonies are picked, five prepared LB agar culture media are additionally taken and inoculated by adopting a plate marking method, a drug sensitive paper sheet which is uniformly wrapped with traditional Chinese medicine compound gel is pasted in the center of the culture medium, the culture is carried out in the water-proof constant temperature box at 37 ℃ for 24 hours, and then observation is carried out.

4. Data analysis processing

The test data is processed by SPSS24.0 software, and standard errors and mean values of canine tooth calculus and dental plaque before and after administration are calculated and expressed by mean value plus or minus standard deviation (M plus or minus SD). And analyzing the canine tooth colorimetric degree by adopting a counting method.

5. Results

5.1, visually measuring the results of dental calculus and dental plaque, comparing and analyzing, and showing in table 1.

TABLE 1 mean length and width of dental calculus and dental plaque in groups A and B

Note: each indicates a significant difference at P <0.05 levels compared to day 0.

As can be seen from Table 1, the mean length of dental calculus and dental plaque in group A was significantly reduced at the administration level 7d compared to the administration level 0 d; the mean length was significantly reduced when 17d was administered compared to 7 d. The width mean value of the A group of dental calculus and dental plaque is obviously reduced when the administration is carried out at 7d and compared with the administration at 0 d; the mean width was significantly reduced when 17d was administered compared to 7 d.

The length mean value of dental calculus and dental plaque in group B is obviously reduced when the drug administration is carried out at 7d and compared with the drug administration at 0 d; the mean length was significantly reduced when 17d was administered compared to 7 d. The width mean value of the dental calculus and dental plaque in the group B is obviously reduced when the drug administration is carried out at 7d and compared with the drug administration at 0 d; the mean width was significantly reduced when 17d was administered compared to 7 d.

The difference between the average length and width of A, B groups before and after administration was calculated, and the average length difference between group A was 1.30mm, the average length difference between group B was 1.63mm, the average width difference between group A was 0.71mm, and the average width difference between group B was 1.50 mm. Thus, the difference between the average lengths of group B before and after administration is larger than the difference between the lengths of group A, and the difference between the average widths of group B before and after administration is larger than the difference between the widths of group A. Comparing the average difference of the length and the width of the A, B groups, the medicine of the B group has better effect. The data analysis was not statistically significantly different, but a significant reduction in mean was observed.

A graph showing the comparison of canine dental calculus and dental plaque in group A dogs No. 1 before and after administration of the gel is shown in FIG. 1.

As can be seen from FIG. 1, the fourth anterior tooth observed with canine drug No. 1 at 17d had a marked lightening in color as compared to drug No. 0 d.

A comparison graph of canine dental calculus and dental plaque of No. 2 dog before and after administration of group B liquid dentifrice gel is shown in FIG. 2.

As seen from FIG. 2, the second anterior tooth and the third anterior tooth observed in 17d of No. 2 canine drug were noticeably lighter in color than in 0 d.

5.2 colorimetric analysis of canine teeth with calculus and dental plaque

The colorimetric analysis of canine teeth according to VITA16 color scale (see FIG. 3) is shown in tables 2-3 below and FIGS. 4-5. FIG. 4 is a histogram comparing the canine tooth color number before and after gel administration of the group A powder. FIG. 5 is a bar graph comparing the canine tooth color number before and after gel administration of the group B solutions.

TABLE 2 statistical table of canine tooth chroma before and after gel administration of group A powder (number of cases/19)

TABLE 3B group of statistics table of canine tooth chroma before and after gel administration (number of cases/19)

As can be seen from tables 2 and 3, the amount of a 1B 1B 2 that is lighter in color after administration is significantly increased compared to before administration, while the amount of a 2B 3B 4 that is darker in color is significantly decreased compared to before administration. Therefore, the traditional Chinese medicine tooth cleaning gel in the examples 3 and 4 has certain effect of reducing the color depth of canine dental calculus and dental plaque.

5.3 treatment of gingival bleeding results and analysis

During the test, gum bleeding of individual dogs caused by external force injury or poor gum health condition of the dogs is observed for multiple times. The dogs were found to have improved gingival bleeding by observation one day after dosing. Thus, the traditional Chinese medicine tooth cleaning gel of the examples 3 and 4 has certain treatment effect on gingival bleeding of dogs.

5.4 results and analysis of drug susceptibility testing

The result of the drug sensitivity test is effective. The obvious bacteriostasis zone can be seen, and the traditional Chinese medicine compound gel is proved to have better bacteriostasis effect.

In conclusion, the medicinal powder tooth-cleaning gel and the medicinal liquid tooth-cleaning gel are prepared by two processes respectively, and compared with the two processes, the preparation process is simple and consumes less time. The experiments show that the medicinal liquid tooth-cleaning gel has more remarkable treatment effect on canine dental calculus and dental plaque than the medicinal powder tooth-cleaning gel. The effect of the Chinese herbal medicines after decoction is generally better than that of the medicines directly ground and pulverized. However, the time for the dog drug solution gel to adhere is not longer than that of the dog drug powder gel, and the proportion of the carbomer 943P to the drug solution in the preparation of the drug solution gel can be continuously discussed and improved.

According to data processing analysis of table 1, the two traditional Chinese medicine tooth cleaning gels have no statistically significant difference on canine dental calculus and dental plaque, but have significant reduction effect on average values of length and width. It is possible that the difference in significance may not have been demonstrated due to too short a dosing cycle, and continued dosing on 17d basis may demonstrate a difference in significance. As shown in the figure 1-2, the traditional Chinese medicine tooth-cleaning gel has the efficacy of lightening the color of dental calculus and dental plaque. The gum health condition of each dog can be observed during the test, the gum red swelling and bleeding symptoms can appear in individual dogs, and the gum bleeding is obviously improved after the test is carried out for one day after the administration, so that the two traditional Chinese medicine tooth cleaning gels have certain treatment effect on the gum bleeding of the dogs. When dog teeth measurement is performed before administration, bad breath of a dog of the size viii is very serious, and at the third measurement, a measurer clearly feels that the dog has an improved oral odor. Therefore, the traditional Chinese medicine tooth-cleaning gel for dogs prepared by the two preparation processes has curative effects on canine dental calculus, dental plaque, gingival inflammation and oral peculiar smell. But the liquid medicine tooth-cleaning gel has better treatment effect on dental calculus and dental plaque.

In conclusion, the medicinal liquid tooth-cleaning gel and the medicinal powder tooth-cleaning gel have certain treatment effects on canine dental calculus, dental plaque, gingival inflammation, oral odor and other comprehensive symptoms. The medicinal liquid tooth-cleaning gel has more obvious treatment effect than the medicinal powder tooth-cleaning gel.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A preparation method of a traditional Chinese medicine tooth-cleaning gel for dogs is characterized in that the traditional Chinese medicine tooth-cleaning gel for dogs is a medicinal powder tooth-cleaning gel or a medicinal liquid tooth-cleaning gel prepared from radix sophorae flavescentis, dried alum, rhizoma drynariae, mint, pseudo-ginseng powder, lactic acid bacteria, tea polyphenol and carbomer;

2. The preparation method of the traditional Chinese medicine tooth-cleaning gel for dogs according to claim 1, wherein the mass ratio of the radix sophorae flavescentis, the dried alum, the rhizoma drynariae, the mint and the pseudo-ginseng powder is 10-30: 1-3: 2-6: 1-3: 2-6.

3. The preparation method of the traditional Chinese medicine tooth-cleaning gel for dogs according to claim 1, wherein in S102, the dosage ratio of carbomer to water is 20 g: 150 and 200 mL.

4. The preparation method of the traditional Chinese medicine tooth-cleaning gel for dogs according to claim 1, wherein in S103, the mass ratio of the traditional Chinese medicine compound powder to the carbomer gel is 40: 180-220.

5. The method for preparing traditional Chinese medicine tooth-cleaning gel for dogs as claimed in claim 1, wherein in S202, the dosage ratio of the traditional Chinese medicine liquid to the carbomer is 150-200 mL: 20 g.

6. The method for preparing a traditional Chinese medicine dentifrice gel for dogs according to claim 1, wherein triethanolamine is used for pH adjustment in S102 and S202.

7. The preparation method of the traditional Chinese medicine tooth-cleaning gel for dogs according to claim 1, wherein the mass ratio of the lactic acid bacteria to the tea polyphenol is 2-4: 1-2.

8. The method for preparing the traditional Chinese medicine tooth-cleaning gel for dogs according to claim 7, wherein in the preparation step of the traditional Chinese medicine tooth-cleaning gel for dogs, after lactic acid bacteria and tea polyphenol are added, pyrophosphate and sorbitol are further added;

wherein the mass ratio of the lactic acid bacteria to the tea polyphenol to the pyrophosphate to the sorbitol is 2-4: 1-2: 40-60: 4-8.

9. The method for preparing the traditional Chinese medicine tooth-cleaning gel for dogs according to claim 1, wherein the carbomer is carbomer 943 p.

10. A traditional Chinese medicine tooth-cleaning gel for dogs prepared according to any one of claims 1-9.