CN113575249A - Seedling growing method for improving medicinal value of forsythia fruits - Google Patents
Seedling growing method for improving medicinal value of forsythia fruits Download PDFInfo
- Publication number
- CN113575249A CN113575249A CN202110793780.XA CN202110793780A CN113575249A CN 113575249 A CN113575249 A CN 113575249A CN 202110793780 A CN202110793780 A CN 202110793780A CN 113575249 A CN113575249 A CN 113575249A
- Authority
- CN
- China
- Prior art keywords
- seeds
- forsythia
- forsythia suspense
- medicinal value
- fruits
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000555682 Forsythia x intermedia Species 0.000 title 1
- 241000555712 Forsythia Species 0.000 claims abstract description 45
- KFFCKOBAHMGTMW-LGQRSHAYSA-N Forsythin Chemical compound C1=C(OC)C(OC)=CC=C1[C@H]1[C@@H](CO[C@@H]2C=3C=C(OC)C(O[C@H]4[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)=CC=3)[C@@H]2CO1 KFFCKOBAHMGTMW-LGQRSHAYSA-N 0.000 claims abstract description 15
- JJVGFDTWFVSBIM-UHFFFAOYSA-N Phillyrin Natural products COc1ccc(cc1OC)C2OCC3C2COC3c4ccc(OC)c(OC5OC(CO)C(O)C(O)C5O)c4 JJVGFDTWFVSBIM-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000007952 growth promoter Substances 0.000 claims abstract description 14
- 238000005507 spraying Methods 0.000 claims abstract description 14
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 12
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 8
- 230000004720 fertilization Effects 0.000 claims abstract description 4
- 238000009331 sowing Methods 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims description 36
- 244000068988 Glycine max Species 0.000 claims description 22
- 235000010469 Glycine max Nutrition 0.000 claims description 22
- 241000233866 Fungi Species 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 241000222290 Cladosporium Species 0.000 claims description 12
- 241000228153 Penicillium citrinum Species 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 230000003213 activating effect Effects 0.000 claims description 9
- 229910021538 borax Inorganic materials 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 9
- 239000008188 pellet Substances 0.000 claims description 9
- 239000004328 sodium tetraborate Substances 0.000 claims description 9
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 9
- 150000001413 amino acids Chemical class 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000004080 punching Methods 0.000 claims description 6
- 239000010231 banlangen Substances 0.000 claims description 5
- 241000334160 Isatis Species 0.000 claims description 4
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 3
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000000084 colloidal system Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000001962 electrophoresis Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 238000007603 infrared drying Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 235000021419 vinegar Nutrition 0.000 claims description 3
- 239000000052 vinegar Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000012856 weighed raw material Substances 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 241000576429 Forsythia suspensa Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 230000004060 metabolic process Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 238000002054 transplantation Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 5
- DTOUWTJYUCZJQD-QJDQKFITSA-N Forsythiaside Natural products C[C@@H]1O[C@H](OC[C@H]2O[C@@H](OCCc3ccc(O)c(O)c3)[C@H](O)[C@@H](O)[C@@H]2OC(=O)C=Cc4ccc(O)c(O)c4)[C@H](O)[C@H](O)[C@H]1O DTOUWTJYUCZJQD-QJDQKFITSA-N 0.000 description 4
- QIMGUQIHCNDUKU-UHFFFAOYSA-N 2-[[6-[2-(3,4-dihydroxyphenyl)ethoxy]-3,4,5-trihydroxyoxan-2-yl]methoxy]-6-methyloxane-3,4,5-triol Chemical compound OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OCCC=2C=C(O)C(O)=CC=2)O1 QIMGUQIHCNDUKU-UHFFFAOYSA-N 0.000 description 2
- DTOUWTJYUCZJQD-UJERWXFOSA-N Forsythiaside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](OC(=O)\C=C\C=2C=C(O)C(O)=CC=2)[C@H](O)[C@@H](O)[C@H](OCCC=2C=C(O)C(O)=CC=2)O1 DTOUWTJYUCZJQD-UJERWXFOSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229930189432 forsythoside Natural products 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 201000000297 Erysipelas Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010025421 Macule Diseases 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 241000207834 Oleaceae Species 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 201000007227 lymph node tuberculosis Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 201000009240 nasopharyngitis Diseases 0.000 description 1
- 201000008383 nephritis Diseases 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention belongs to the technical field of plant cultivation, and discloses a seedling growing method for improving the medicinal value of forsythia fruits, which comprises the following steps: selecting strong fructus forsythiae mother tree in the middle and late ten days of 6 months, collecting mature fruit with full seeds, and shelling to obtain fructus forsythiae seeds; treating the obtained forsythia suspense seeds; sowing the treated seeds into a planting field loosened in advance, and spraying the prepared bean sprout juice into the planting field; when the seeds germinate, spraying a pre-prepared growth promoter to the roots and leaves of the forsythia suspense, and performing fertilization, watering and other field management. The invention can enhance the nutrition metabolism capability of forsythia suspense seedlings, improve the absorption, utilization and conversion capability of nutrients, effectively promote the robust growth of forsythia suspense seedlings, effectively culture strong seedlings, improve the survival rate and growth capability after transplantation, effectively improve the phillyrin content of forsythia suspense fruits and improve the medicinal value of forsythia suspense fruits.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a seedling growing method for improving the medicinal value of forsythia fruits.
Background
At present: fructus forsythiae is the dry fruit of fructus forsythiae of forsythia of Oleaceae, has the effects of clearing away heat and toxic material, reducing swelling and resolving masses, is mainly used for treating diseases such as tepidity, erysipelas, macula, carbuncle and ulcer pyogenic infections, scrofula, urinary stranguria and the like, and is clinically used for treating diseases such as respiratory tract infection, acute nephritis, hepatitis, meningitis and the like; modern pharmacological research shows that fructus forsythiae has the effects of resisting bacteria, inflammation and heat, forsythoside has the obvious effects of resisting oxidation and microbial infection, eliminating free radicals and relieving peroxidation injury, and forsythoside has the effects of clearing heat, detoxifying, dissipating stagnation and expelling pus and the like and is commonly used for wind-heat type common cold; the method for improving the content of forsythiaside and forsythiaside in fructus forsythiae fruits can effectively improve the medicinal value of fructus forsythiae fruits, further promote the further high-speed development of fructus forsythiae industries and increase the economic value of fructus forsythiae fruits.
Through the above analysis, the problems and defects of the prior art are as follows: the fructus forsythiae cultivated by the existing fructus forsythiae seedling culture method has low content of effective components and unstable quality.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a seedling raising method for improving the medicinal value of forsythia fruits.
The invention is realized in such a way that a seedling raising method for improving the medicinal value of forsythia fruits comprises the following steps:
selecting a strong fructus forsythiae mother tree in the middle and late ten days of 6 months, collecting mature fruits with full seeds, and carrying out shelling treatment to obtain fructus forsythiae seeds;
step two, treating the obtained forsythia suspense seeds; sowing the treated seeds into a planting field loosened in advance, and spraying the prepared bean sprout juice into the planting field;
and step three, when the seeds germinate, spraying a pre-prepared growth promoter to the roots and leaves of the forsythia suspense, and performing fertilization, watering and other field management.
Further, in the second step, the processing of the obtained forsythia suspense seeds comprises:
(1) spraying a layer of white vinegar on the surfaces of the forsythia seeds, and then placing the forsythia seeds in a stable magnetic field for treatment;
(2) preparing a seed soaking solution, placing the seed treated by the magnetic field in an electrophoresis tank, wrapping the seed in a gauze bag, soaking the seed in the seed soaking solution, fixing the seed soaking solution at an anode end, switching on a power supply, wherein a direct current power supply is 6-8V, and the seed soaking treatment time is 1-2 hours;
(3) drying the soaked seeds in an infrared drying oven; and (4) placing the dried seeds in a constant temperature box for low-temperature treatment.
Further, the intensity of the steady magnetic field is 1400-1600Gs, and the time of the magnetic field treatment is 8-10 min.
Further, in the step (2), the seed soaking liquid comprises, by mass, 25-30 parts of pearl powder, 15-20 parts of amino acid chelating liquid, 5-8 parts of isatis root extract, 10-13 parts of borax and the balance of water.
Further, the seed soaking liquid preparation method comprises the following steps:
preparing radix Isatidis extract, and pulverizing Borax; weighing pearl powder, radix Isatidis extract, and pulverized borax according to a certain proportion;
uniformly mixing the weighed raw materials, adding water, soaking at room temperature for 1-2h, and filtering to obtain a soaking solution;
and combining the soaking solution and the amino acid chelating solution, and concentrating to obtain the soaking solution.
Further, in the step (3), the wavelength of the infrared is controlled to be 20-25 μm.
Further, the performing low-temperature treatment includes: controlling the temperature in the constant temperature box at 8-10 deg.C, and treating at low temperature for 8-10 min.
Further, the bean sprout juice preparation comprises:
selecting soybeans, soaking the soybeans in clear water for 4-6h, washing the soybeans with clear water when the soybeans swell, covering the soybeans with moist gauze, then putting the soybeans in an incubator at 25-30 ℃ for culturing and sprouting, and spraying the clear water once every morning and evening;
and after 3-5 days of sprouting, taking out buds of 3-7cm, cleaning, draining, adding deionized water of which the weight is 1.5-2.5 times that of the buds, and grinding the mixture by using a colloid mill to obtain soybean sprout juice.
Furthermore, the growth promoter consists of cladosporium dendritic LQ-1, phillyrin endophytic fungus G10 and Penicillium citrinum LB strain.
Further, the preparation method of the growth promoter comprises the following steps:
activating dendritic cladosporium LQ-1 on an improved culture medium, punching after hyphae grow over the surface of the culture medium, inoculating a fungus cake into a culture solution, performing shake fermentation culture at constant temperature for 6-8 days, filtering mycelium pellets, and retaining filtrate to obtain the dendritic cladosporium LQ-1;
activating phillyrin endophytic fungus G10 on an improved culture medium, perforating after hyphae grow over the surface of the culture medium, inoculating fungus cakes into a culture solution, performing shake fermentation at constant temperature for 8-10d, filtering mycelium pellets, and retaining filtrate to obtain the phillyrin endophytic fungus G10;
activating the penicillium citrinum LB strain on an improved culture medium, punching after hyphae grow over the surface of the culture medium, inoculating a fungus cake into the improved culture solution, performing shake fermentation at constant temperature for 9-11d, filtering mycelium pellets, and retaining filtrate to obtain the penicillium citrinum LB strain;
at room temperature, adding dendritic cladosporium LQ-1, phillyrin endophytic fungi G10 and penicillium citrinum LB strains into a container in sequence according to a ratio, and shaking uniformly to obtain the growth promoter.
Further, the improved medium is composed of 8 g.L-1Glucose, 1 mg. L-1Yeast extract, 1 mg. L-12,4-D、0.5mg·L-1KT and 0.5 mg. L-16-BA.
By combining all the technical schemes, the invention has the advantages and positive effects that: the invention can enhance the nutrition metabolism capability of forsythia suspense seedlings, improve the absorption, utilization and conversion capability of nutrients, effectively promote the robust growth of forsythia suspense seedlings, effectively culture strong seedlings, improve the survival rate and growth capability after transplantation, effectively improve the phillyrin content of forsythia suspense fruits and improve the medicinal value of forsythia suspense fruits.
The method has the advantages that the forsythia seeds are pretreated, so that the dormancy of the seeds can be effectively relieved, the activity of the seeds is enhanced, the activity of the seeds is improved, the seeds are promoted to germinate and emerge quickly, the seedlings are aligned and strong, the quick rooting of the forsythia plants is promoted, the quick growth, development and extension and expansion of root systems are promoted, the activity of the forsythia plants can be effectively improved, and the content of phillyrin is obviously improved; the invention combines the soybean juice and the growth promoter, can change the biological characteristics of the forsythia, promote the growth of forsythia apricots, improve the capability of the forsythia to resist adverse environment, promote the synthesis and accumulation of effective components of the forsythia, greatly improve the economic value and the medicinal value and have better application prospect.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a seedling growing method for improving the medicinal value of forsythia fruits provided by the embodiment of the invention.
FIG. 2 is a flow chart of a method for treating the obtained forsythia suspense seeds according to an embodiment of the present invention.
FIG. 3 is a flow chart of a method for preparing seed soaking liquid according to an embodiment of the present invention.
Fig. 4 is a flow chart of a method for preparing bean sprout juice according to an embodiment of the present invention.
FIG. 5 is a flow chart of a method for preparing growth promoting agent provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a seedling raising method for improving the medicinal value of forsythia fruits, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the seedling raising method for improving the medicinal value of forsythia fruits provided by the embodiment of the invention comprises the following steps:
s101, selecting a strong fructus forsythiae mother tree in the middle and late ten days of 6 months, collecting mature fruits with full seeds, and carrying out shelling treatment to obtain fructus forsythiae seeds;
s102, treating the obtained fructus forsythiae seeds; sowing the treated seeds into a planting field loosened in advance, and spraying the prepared bean sprout juice into the planting field;
s103, when the seeds germinate, spraying a pre-prepared growth promoter to the roots and leaves of the fructus forsythiae, and performing fertilization, watering and other field management.
As shown in fig. 2, the processing of the obtained forsythia suspense seeds according to the embodiment of the present invention includes:
s201, spraying a layer of white vinegar on the surfaces of fructus forsythiae seeds, and then placing the fructus forsythiae seeds in a steady magnetic field for treatment;
s202, preparing seed soaking liquid, placing the seed treated by the magnetic field in an electrophoresis tank, wrapping the seed in a gauze bag, soaking the seed in the seed soaking liquid and fixing the seed in an anode end, and switching on a power supply, wherein a direct current power supply is 6-8V, and the seed soaking treatment time is 1-2 hours;
s203, placing the soaked seeds in an infrared drying oven for drying; and (4) placing the dried seeds in a constant temperature box for low-temperature treatment.
The intensity of the steady magnetic field provided by the embodiment of the invention is 1400-1600Gs, and the time for magnetic field treatment is 8-10 min.
The soaking liquid provided by the embodiment of the invention comprises, by mass, 25-30 parts of pearl powder, 15-20 parts of amino acid chelating liquid, 5-8 parts of isatis root extract, 10-13 parts of borax and the balance of water.
As shown in fig. 3, the method for preparing the seed soaking liquid provided by the embodiment of the invention includes:
s301, preparing an isatis root extract, and crushing borax; weighing pearl powder, radix Isatidis extract, and pulverized borax according to a certain proportion;
s302, uniformly mixing the weighed raw materials, adding water, soaking at room temperature for 1-2 hours, and filtering to obtain a soaking solution;
and S303, combining the soaking solution and the amino acid chelating solution, and concentrating to obtain the soaking solution.
The wavelength of infrared provided by the embodiment of the invention is controlled to be 20-25 μm.
The low-temperature treatment provided by the embodiment of the invention comprises the following steps: controlling the temperature in the constant temperature box at 8-10 deg.C, and treating at low temperature for 8-10 min.
As shown in fig. 4, the preparation of bean sprout juice according to the embodiment of the present invention includes:
s401, selecting soybeans, soaking the soybeans in clear water for 4-6h, washing the soybeans with clear water when the soybeans swell, covering the soybeans with moist gauze, then putting the soybeans in an incubator at 25-30 ℃ for culturing and sprouting, and spraying the clear water once every morning and evening;
s402, after 3-5 days of sprouting, taking out 3-7cm sprouts, cleaning, draining, adding deionized water with the weight 1.5-2.5 times of the weight of the sprouts, and grinding the mixture by using a colloid mill to obtain soybean sprout juice.
The growth promoter provided by the embodiment of the invention consists of cladosporium dendritic LQ-1, phillyrin endophytic fungus G10 and penicillium citrinum LB strain.
As shown in fig. 5, the preparation method of the growth promoter provided by the embodiment of the present invention includes:
s501, activating dendritic cladosporium LQ-1 on an improved culture medium, punching after hyphae grow over the surface of the culture medium, inoculating a fungus cake into a culture solution, performing shake fermentation culture at constant temperature for 6-8 days, filtering mycelium pellets, and retaining filtrate to obtain the dendritic cladosporium LQ-1;
s502, activating phillyrin endophytic fungus G10 on an improved culture medium, punching after hyphae grow over the surface of the culture medium, inoculating fungus cakes into a culture solution, performing shake fermentation at a constant temperature for 8-10 days, filtering mycelium pellets, and retaining filtrate to obtain the phillyrin endophytic fungus G10;
s503, activating the Penicillium citrinum LB strain on an improved culture medium, perforating after hyphae grow over the surface of the culture medium, inoculating a bacterial cake into the improved culture solution, performing shake fermentation at constant temperature for 9-11 days, filtering mycelium pellets, and retaining filtrate to obtain the Penicillium citrinum LB strain;
s504, adding dendritic cladosporium LQ-1, phillyrin endophytic fungi G10 and penicillium citrinum LB strains into a container in sequence according to the proportion at room temperature, and vibrating uniformly to obtain the growth promoter.
The improved culture medium provided by the embodiment of the invention is composed of 8 g.L-1Glucose, 1 mg. L-1Yeast extract, 1 mg. L-12,4-D、0.5mg·L-1KT and 0.5 mg. L-16-BA.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (10)
1. A seedling raising method for improving the medicinal value of forsythia fruits is characterized by comprising the following steps:
selecting a strong fructus forsythiae mother tree in the middle and late ten days of 6 months, collecting mature fruits with full seeds, and carrying out shelling treatment to obtain fructus forsythiae seeds;
step two, treating the obtained forsythia suspense seeds; sowing the treated seeds into a planting field loosened in advance, and spraying the prepared bean sprout juice into the planting field;
and step three, when the seeds germinate, spraying a pre-prepared growth promoter to the roots and leaves of the forsythia suspense, and performing fertilization, watering and other field management.
2. The method for improving the medicinal value of forsythia suspense fruit as claimed in claim 1, wherein in the second step, the treating the forsythia suspense seeds comprises:
(1) spraying a layer of white vinegar on the surfaces of the forsythia seeds, and then placing the forsythia seeds in a stable magnetic field for treatment;
(2) preparing a seed soaking solution, placing the seed treated by the magnetic field in an electrophoresis tank, wrapping the seed in a gauze bag, soaking the seed in the seed soaking solution, fixing the seed soaking solution at an anode end, switching on a power supply, wherein a direct current power supply is 6-8V, and the seed soaking treatment time is 1-2 hours;
(3) drying the soaked seeds in an infrared drying oven; and (4) placing the dried seeds in a constant temperature box for low-temperature treatment.
3. The method as claimed in claim 2, wherein the intensity of the steady magnetic field is 1400-1600Gs, and the time for the magnetic field treatment is 8-10 min.
4. The seedling raising method for improving the medicinal value of forsythia suspense fruits according to claim 2, wherein in the step (2), the seed soaking solution is composed of, by mass, 25-30 parts of pearl powder, 15-20 parts of amino acid chelating solution, 5-8 parts of isatis root extract, 10-13 parts of borax and the balance of water.
5. The method for improving the medicinal value of forsythia suspense fruits according to claim 2, wherein the method for preparing the seed soaking solution comprises:
preparing radix Isatidis extract, and pulverizing Borax; weighing pearl powder, radix Isatidis extract, and pulverized borax according to a certain proportion;
uniformly mixing the weighed raw materials, adding water, soaking at room temperature for 1-2h, and filtering to obtain a soaking solution;
and combining the soaking solution and the amino acid chelating solution, and concentrating to obtain the soaking solution.
6. The method for improving the medicinal value of forsythia suspense fruit as claimed in claim 2, wherein in the step (3), the wavelength of the infrared ray is controlled to be 20 to 25 μm.
7. The method for improving the medicinal value of forsythia suspensa fruit as claimed in claim 1, wherein the performing the low temperature treatment comprises: controlling the temperature in the constant temperature box at 8-10 deg.C, and treating at low temperature for 8-10 min.
8. The method of claim 1, wherein the preparing of the bean sprout juice comprises:
selecting soybeans, soaking the soybeans in clear water for 4-6h, washing the soybeans with clear water when the soybeans swell, covering the soybeans with moist gauze, then putting the soybeans in an incubator at 25-30 ℃ for culturing and sprouting, and spraying the clear water once every morning and evening;
and after 3-5 days of sprouting, taking out buds of 3-7cm, cleaning, draining, adding deionized water of which the weight is 1.5-2.5 times that of the buds, and grinding the mixture by using a colloid mill to obtain soybean sprout juice.
9. The method for improving the medicinal value of forsythia suspense fruits according to claim 1, wherein the growth promoter comprises cladosporium dendritic LQ-1, phillyrin endophytic fungus G10, and penicillium citrinum LB strain.
10. The method for improving the medicinal value of forsythia suspense fruits according to claim 1, wherein the growth promoter is prepared by a method comprising:
activating dendritic cladosporium LQ-1 on an improved culture medium, punching after hyphae grow over the surface of the culture medium, inoculating a fungus cake into a culture solution, performing shake fermentation culture at constant temperature for 6-8 days, filtering mycelium pellets, and retaining filtrate to obtain the dendritic cladosporium LQ-1;
activating phillyrin endophytic fungus G10 on an improved culture medium, perforating after hyphae grow over the surface of the culture medium, inoculating fungus cakes into a culture solution, performing shake fermentation at constant temperature for 8-10d, filtering mycelium pellets, and retaining filtrate to obtain the phillyrin endophytic fungus G10;
activating the penicillium citrinum LB strain on an improved culture medium, punching after hyphae grow over the surface of the culture medium, inoculating a fungus cake into the improved culture solution, performing shake fermentation at constant temperature for 9-11d, filtering mycelium pellets, and retaining filtrate to obtain the penicillium citrinum LB strain;
sequentially adding dendritic cladosporium LQ-1, phillyrin endophytic fungus G10 and penicillium citrinum LB strains into a container according to a ratio at room temperature, and uniformly shaking to obtain the growth promoter;
the improved culture medium consists of 8 g.L-1Glucose, 1 mg. L-1Yeast extract, 1 mg. L-12,4-D、0.5mg·L-1KT and 0.5 mg. L-16-BA.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110793780.XA CN113575249A (en) | 2021-07-14 | 2021-07-14 | Seedling growing method for improving medicinal value of forsythia fruits |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110793780.XA CN113575249A (en) | 2021-07-14 | 2021-07-14 | Seedling growing method for improving medicinal value of forsythia fruits |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113575249A true CN113575249A (en) | 2021-11-02 |
Family
ID=78247251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110793780.XA Pending CN113575249A (en) | 2021-07-14 | 2021-07-14 | Seedling growing method for improving medicinal value of forsythia fruits |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113575249A (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102115347A (en) * | 2010-12-12 | 2011-07-06 | 夏学德 | Mixture fertilizer and preparation method thereof |
| CN104293678A (en) * | 2014-04-14 | 2015-01-21 | 河南科技大学 | Forsythia endophytic fungi capable of producing forsythoside A and B and forsythin, and application thereof |
| CN106105780A (en) * | 2016-07-07 | 2016-11-16 | 孙备宽 | A kind of Se-rich pearl Cordyceps production method |
| CN106134875A (en) * | 2016-06-28 | 2016-11-23 | 包宗辉 | A kind of cultural method of the rich red rice of magnesium |
| CN106212176A (en) * | 2016-07-31 | 2016-12-14 | 普定县远山林下养鸡场 | A kind of peach tree implantation methods |
| CN106282032A (en) * | 2016-08-11 | 2017-01-04 | 浙江工业大学 | Aspergillus citrimum LB and prepare the application in phillygenol at bioconversion phillyrin |
| CN106576837A (en) * | 2016-12-18 | 2017-04-26 | 钦州市科学技术开发中心 | Planting method of onions |
| CN107382426A (en) * | 2017-08-28 | 2017-11-24 | 平南县正达农业发展有限公司 | A kind of organic rice implantation methods for improving rice total amino acid content |
| CN108370977A (en) * | 2016-10-25 | 2018-08-07 | 甘博 | A kind of cultural method of richness 916 miscellaneous excellent water rice of magnesium |
| CN109105125A (en) * | 2018-08-15 | 2019-01-01 | 马鞍山市绿营林业有限公司 | A kind of method for culturing seedlings of apocarya |
| CN109197304A (en) * | 2018-07-18 | 2019-01-15 | 枞阳县天腾生态农业有限公司 | A kind of method for culturing seedlings promoting Fructus Forsythiae fruit medical value |
| CN111642339A (en) * | 2020-07-03 | 2020-09-11 | 三亚双盛实业有限公司 | Hami melon seedling culture method |
| CN112646732A (en) * | 2020-12-24 | 2021-04-13 | 湖南科技学院 | Growth promoting preparation capable of simultaneously increasing content of lactone A, C and bilobalide in ginkgo leaf and preparation method and application thereof |
-
2021
- 2021-07-14 CN CN202110793780.XA patent/CN113575249A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102115347A (en) * | 2010-12-12 | 2011-07-06 | 夏学德 | Mixture fertilizer and preparation method thereof |
| CN104293678A (en) * | 2014-04-14 | 2015-01-21 | 河南科技大学 | Forsythia endophytic fungi capable of producing forsythoside A and B and forsythin, and application thereof |
| CN106134875A (en) * | 2016-06-28 | 2016-11-23 | 包宗辉 | A kind of cultural method of the rich red rice of magnesium |
| CN106105780A (en) * | 2016-07-07 | 2016-11-16 | 孙备宽 | A kind of Se-rich pearl Cordyceps production method |
| CN106212176A (en) * | 2016-07-31 | 2016-12-14 | 普定县远山林下养鸡场 | A kind of peach tree implantation methods |
| CN106282032A (en) * | 2016-08-11 | 2017-01-04 | 浙江工业大学 | Aspergillus citrimum LB and prepare the application in phillygenol at bioconversion phillyrin |
| CN108370977A (en) * | 2016-10-25 | 2018-08-07 | 甘博 | A kind of cultural method of richness 916 miscellaneous excellent water rice of magnesium |
| CN106576837A (en) * | 2016-12-18 | 2017-04-26 | 钦州市科学技术开发中心 | Planting method of onions |
| CN107382426A (en) * | 2017-08-28 | 2017-11-24 | 平南县正达农业发展有限公司 | A kind of organic rice implantation methods for improving rice total amino acid content |
| CN109197304A (en) * | 2018-07-18 | 2019-01-15 | 枞阳县天腾生态农业有限公司 | A kind of method for culturing seedlings promoting Fructus Forsythiae fruit medical value |
| CN109105125A (en) * | 2018-08-15 | 2019-01-01 | 马鞍山市绿营林业有限公司 | A kind of method for culturing seedlings of apocarya |
| CN111642339A (en) * | 2020-07-03 | 2020-09-11 | 三亚双盛实业有限公司 | Hami melon seedling culture method |
| CN112646732A (en) * | 2020-12-24 | 2021-04-13 | 湖南科技学院 | Growth promoting preparation capable of simultaneously increasing content of lactone A, C and bilobalide in ginkgo leaf and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 方子卫等: "中微量元素肥料硼与锌对水稻生产的影响及施用技术", 《现代农业科技》 * |
| 马保芳: "林州市无公害生姜高产栽培技术", 《河南农业》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101870608B (en) | Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof | |
| CN102030581B (en) | Novel multi-functional biological organic seed dressing agent and method for preparing same | |
| CN106688849A (en) | Soilless culture method of Chinese flowering cabbage | |
| CN107047165A (en) | A kind of implantation methods of high-yield rice | |
| CN111807911B (en) | Soil microorganism regulator for enhancing plant stress resistance | |
| CN107079711B (en) | Cultivation method of morchella | |
| AU2020102508A4 (en) | A one-step seedling culture medium for in vitro culture of Cyperus esculentus L. | |
| CN105724011A (en) | Planting and cultivating method for selenium-enriched peony | |
| CN108033858B (en) | Special clay soil conditioner for facility agriculture | |
| CN114195598B (en) | Microalgae fertilizer for improving soil fertility and preparation method and application thereof | |
| CN103725626B (en) | Bacillus laterosporus preparation | |
| CN110627582A (en) | Preparation method of solid carbon-based fertilizer and liquid pesticide-fertilizer integrated preparation | |
| CN110036851B (en) | Pinellia ternata water-drought segmented seed breeding method | |
| CN111657148A (en) | Grass seed detoxification treatment method | |
| CN107396748A (en) | A kind of cultural method of Se-rich xianggu | |
| CN114931150B (en) | Cultivation method for improving medicinal quality of cyclocarya paliurus leaves by using trichoderma asperellum | |
| CN106305107A (en) | Seedling growing method of Longzhao balsamine | |
| CN106576741A (en) | Cultivation method of cabbages with high production capacity | |
| CN113575249A (en) | Seedling growing method for improving medicinal value of forsythia fruits | |
| CN107820779A (en) | A kind of modification processing method of corn seed | |
| CN109928855A (en) | One kind is rich in potassium fulvate type microbial bacterial agent and preparation method thereof | |
| CN112979361A (en) | Soilless culture nutrition gel | |
| CN115053915B (en) | Cultivation method for improving medicinal quality of cyclocarya paliurus leaves by using bacillus | |
| CN111279994B (en) | Method for cultivating tetraploid dandelion | |
| CN114451098B (en) | Method for promoting germination of pepper seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211102 |