CN113545368B - Growth regulator for potatoes and preparation method thereof - Google Patents
Growth regulator for potatoes and preparation method thereof Download PDFInfo
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- CN113545368B CN113545368B CN202110822136.0A CN202110822136A CN113545368B CN 113545368 B CN113545368 B CN 113545368B CN 202110822136 A CN202110822136 A CN 202110822136A CN 113545368 B CN113545368 B CN 113545368B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention discloses a growth regulator for potatoes, which comprises benayurine, choline chloride and acacia flower-moringa oleifera leaf-hawthorn composite extract, wherein the preparation method of the acacia flower-moringa oleifera leaf-hawthorn composite extract comprises the following steps: (1) mixing flos Sophorae Immaturus petal, Moringa oleifera leaf and fructus crataegi pulp, chopping, adding deionized water, pressurizing, standing, and vacuumizing; (2) adding a mixed aqueous solution of sodium citrate, glycine and acetic acid, extracting in a water bath at constant temperature, and filtering to obtain a filtrate A and filter residues; (3) placing filter residues in a container, adding deionized water, sealing the container, heating to 140 +/-5 ℃, preserving heat for 20-30 min, cooling to normal temperature in air, and filtering to obtain filtrate B; (4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to obtain the sophora japonica-moringa oleifera leaf-hawthorn composite extract. The growth regulator of the invention has obvious growth promoting effect on potatoes, and the yield increasing rate and the starch content of the potatoes are obviously improved after the growth regulator is applied.
Description
Technical Field
The invention relates to the technical field of vegetable planting, in particular to a growth regulator for potatoes and a preparation method thereof.
Background
The potato serving as the 4 th large grain crop has the characteristics of wide planting distribution, multiple application and use, high functional value and the like, is an important dual-purpose crop of grain, vegetable and feed, is an indispensable raw material for the light industry, the food industry and the medicine manufacturing industry, increases the potato planting area of China and countries all over the world year by year, and has good industrial economic chain development situation.
In recent years, a technology of regulating and controlling the endogenous hormone level of plants and the proportional relation among hormones by spraying a plant growth regulator is widely applied, and the growth and development of crops and physiological metabolic processes, such as photosynthesis, respiration, stomatal index, photosynthetic enzyme activity and the like of the crops, are further controlled by the growth regulator. In addition, the plant growth regulator also has an important effect on the carbon metabolism process of crops, and the regulator can regulate and control the starch metabolism process in tubers, so that the yield of the tubers is increased, and the quality of the tubers is improved.
Disclosure of Invention
Therefore, the invention provides a growth regulator for potatoes, which comprises benaylaminopurine, choline chloride and acacia flower-moringa oleifera leaf-hawthorn fruit composite extract, wherein the preparation method of the acacia flower-moringa oleifera leaf-hawthorn fruit composite extract comprises the following steps:
(1) Mixing and chopping sophora japonica petals, moringa oleifera leaves and hawthorn pulp, placing chopped diced meat into a container, adding deionized water into the container, sealing the container, pressurizing to 8-10 standard atmospheric pressures, standing for 10-20 min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing the container into a vacuum box, vacuumizing until the pressure is less than or equal to 0.01 standard atmospheric pressure, maintaining the pressure for 5-6 min, and taking out the container;
(2) preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into the container, extracting for 2-3 h in a water bath at the temperature of 75 +/-5 ℃ at constant temperature, then cooling to the normal temperature, and filtering to obtain a filtrate A and filter residues;
(3) placing the filter residue in a container, adding deionized water into the container, sealing the container, heating to 140 +/-5 ℃, preserving heat for 20-30 min, air-cooling to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to obtain the sophora japonica-moringa oleifera leaf-hawthorn composite extract.
Further, the growth regulator comprises the following components in parts by weight: 10 parts of benzylaminopurine, 2-6 parts of choline chloride and 4-9 parts of acacia flower-moringa leaf-hawthorn composite extract.
Further, the mixing mass ratio of the acacia flower petals to the moringa leaves to the hawthorn pulp is as follows: and (3) horseradish tree leaves: the ratio of hawthorn pulp to 1: 0.3-0.8: 0.1-0.2.
Further, in the mixed water solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 1-3 g/100mL, the concentration of glycine is 0.2-0.4 g/100mL, the concentration of acetic acid is 0.2-0.4 g/100mL, and the balance is water; and adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into a container, wherein the mass of the mixed aqueous solution is 7-8 times of the total mass of the mixed diced leaves of acacia flower petals, moringa leaves and hawthorn fruit pulp.
Further, in the step (3), adding deionized water into the container, wherein the mass of the deionized water is 5-6 times of that of filter residues in the container; and (4) concentrating the mixed solution of the filtrate A and the filtrate B under reduced pressure to 1/10-1/9 of the volume before concentration to obtain the robinia pseudoacacia flower-moringa oleifera leaf-hawthorn fruit composite extract.
The invention also discloses an application method of the growth regulator, the growth regulator is mixed with water to prepare a medicament, and the medicament is sprayed in a seedling stage of potatoes, wherein the spraying amount is 200-300 mL/plant.
The water adding proportion of the growth regulator is 120-180 mg/L.
The invention has the beneficial effects that: the growth regulator has obvious growth promotion effect on potatoes, the yield of the potatoes is obviously increased after the growth regulator is applied, and the content of starch in the potatoes is obviously higher than that in a control group without the growth regulator, so that the growth regulator has the effect of improving the yield and the quality of the potatoes.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
A growth regulator for potatoes comprises benayurine, choline chloride and acacia flower-moringa oleifera leaf-hawthorn composite extract, and comprises the following components in parts by weight: 10 parts of benzylaminopurine, 2 parts of choline chloride and 4 parts of acacia flower-moringa oleifera leaf-hawthorn fruit composite extract.
The preparation method of the acacia flower-moringa oleifera leaf-hawthorn composite extract comprises the following steps:
(1) mixing the sophora japonica flower petals in a mixing mass ratio: and (3) horseradish tree leaves: mixing acacia flower petals, moringa oleifera leaves and hawthorn pulp in a ratio of 1:0.3:0.1, chopping, putting the chopped diced pieces into a container, and adding deionized water into the container, wherein the addition amount of the deionized water is 1.6 times of the total mass of the diced pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) Preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, wherein in the mixed aqueous solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 1g/100mL, the concentration of glycine is 0.2g/100mL, the concentration of acetic acid is 0.2g/100mL, and the balance is water; adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into the container, wherein the added mass of the mixed aqueous solution of sodium citrate, glycine and acetic acid is 7 times of the total mass of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp mixed diced pieces, extracting for 2 hours in a water bath at the temperature of 75 +/-5 ℃, then cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 20min, cooling to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the acacia flower-moringa oleifera leaf-hawthorn fruit compound extract.
Example 2
A growth regulator for potatoes comprises benayurine, choline chloride and acacia flower-moringa oleifera leaf-hawthorn composite extract, and comprises the following components in parts by weight: 10 parts of benzylaminopurine, 3 parts of choline chloride and 5 parts of acacia flower-moringa leaf-hawthorn composite extract.
The preparation method of the acacia flower-moringa oleifera leaf-hawthorn composite extract comprises the following steps:
(1) mixing the sophora japonica flower petals in a mixing mass ratio: and (3) horseradish tree leaves: mixing acacia flower petals, moringa leaves and hawthorn pulp in a ratio of 1:0.5:0.1, chopping, putting the chopped diced pieces into a container, and adding deionized water into the container, wherein the addition amount of the deionized water is 1.6 times of the total mass of the diced pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, wherein in the mixed aqueous solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 2g/100mL, the concentration of glycine is 0.3g/100mL, the concentration of acetic acid is 0.3g/100mL, and the balance is water; adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into the container, wherein the added mass of the mixed aqueous solution of sodium citrate, glycine and acetic acid is 7 times of the total mass of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp mixed diced pieces, extracting for 2 hours in a water bath at the temperature of 75 +/-5 ℃, then cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) Placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 20min, cooling in air to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the acacia flower-moringa oleifera leaf-hawthorn fruit composite extract.
Example 3
A growth regulator for potatoes comprises benayurine, choline chloride and acacia flower-moringa oleifera leaf-hawthorn composite extract, and comprises the following components in parts by weight: 10 parts of benzylaminopurine, 4 parts of choline chloride and 7 parts of acacia flower-moringa oleifera leaf-hawthorn fruit composite extract.
The preparation method of the acacia flower-moringa oleifera leaf-hawthorn composite extract comprises the following steps:
(1) mixing the sophora japonica flower petals in a mixing mass ratio: and (3) horseradish tree leaves: mixing sophora japonica flower petals, moringa oleifera leaves and hawthorn pulp in a ratio of 1:0.7:0.2, chopping, putting the chopped diced pieces into a container, and adding deionized water into the container, wherein the addition amount of the deionized water is 1.6 times of the total mass of the diced pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) Preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, wherein in the mixed aqueous solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 2g/100mL, the concentration of glycine is 0.3g/100mL, the concentration of acetic acid is 0.3g/100mL, and the balance is water; adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into the container, wherein the added mass of the mixed aqueous solution of sodium citrate, glycine and acetic acid is 7 times of the total mass of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp mixed diced pieces, extracting for 2 hours in a water bath at the temperature of 75 +/-5 ℃, then cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 30min, cooling to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the acacia flower-moringa oleifera leaf-hawthorn fruit composite extract.
Example 4
A growth regulator for potatoes comprises benayurine, choline chloride and acacia flower-moringa oleifera leaf-hawthorn composite extract, and comprises the following components in parts by weight: 10 parts of benzylaminopurine, 6 parts of choline chloride and 9 parts of acacia flower-moringa oleifera leaf-hawthorn fruit composite extract.
The preparation method of the acacia flower-moringa oleifera leaf-hawthorn composite extract comprises the following steps:
(1) mixing the sophora japonica flower petals in a mixing mass ratio: and (3) horseradish tree leaves: mixing sophora japonica flower petals, moringa oleifera leaves and hawthorn pulp in a ratio of 1:0.8:0.2, chopping, putting the chopped diced pieces into a container, and adding deionized water into the container, wherein the addition amount of the deionized water is 1.6 times of the total mass of the diced pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, wherein in the mixed aqueous solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 3g/100mL, the concentration of glycine is 0.4g/100mL, the concentration of acetic acid is 0.4g/100mL, and the balance is water; adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into the container, wherein the added mass of the mixed aqueous solution of sodium citrate, glycine and acetic acid is 7 times of the total mass of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp mixed diced pieces, extracting for 2 hours in a water bath at the temperature of 75 +/-5 ℃, then cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) Placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 30min, cooling to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the acacia flower-moringa oleifera leaf-hawthorn fruit compound extract.
Comparative example 1
A growth regulator for potatoes comprises benzylaminopurine and choline chloride, and comprises the following components in parts by weight: 10 parts of benzylaminopurine and 4 parts of choline chloride.
Comparative example 2
A growth regulator for potatoes comprises benayurine, choline chloride and acacia flower extract, and comprises the following components in parts by weight: 10 parts of benzylaminopurine, 4 parts of choline chloride and 7 parts of sophora japonica extract.
The preparation method of the sophora japonica extract comprises the following steps:
(1) cutting up acacia flower petals, putting the cut-up diced pieces into a container, and adding deionized water into the container, wherein the adding amount of the deionized water is 1.6 times of the total mass of the diced pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) Preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, wherein in the mixed aqueous solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 2g/100mL, the concentration of glycine is 0.3g/100mL, the concentration of acetic acid is 0.3g/100mL, and the balance is water; adding the mixed aqueous solution of the sodium citrate, the glycine and the acetic acid into the container, wherein the added mass of the mixed aqueous solution of the sodium citrate, the glycine and the acetic acid is 7 times of the total mass of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp mixed diced meat, performing water bath constant-temperature extraction at 75 +/-5 ℃ for 2 hours, then performing air cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 30min, cooling in air to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the acacia flower extract of the comparative example.
Comparative example 3
A growth regulator for potatoes comprises benayl aminopurine, choline chloride and a moringa oleifera leaf extract, and comprises the following components in parts by weight: 10 parts of benzylaminopurine, 4 parts of choline chloride and 7 parts of moringa oleifera leaf extract.
The preparation method of the moringa oleifera leaf extract comprises the following steps:
(1) cutting moringa leaves into pieces, putting the cut pieces into a container, and adding deionized water into the container, wherein the adding amount of the deionized water is 1.6 times of the total mass of the pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, wherein in the mixed aqueous solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 2g/100mL, the concentration of glycine is 0.3g/100mL, the concentration of acetic acid is 0.3g/100mL, and the balance is water; adding the mixed aqueous solution of the sodium citrate, the glycine and the acetic acid into the container, wherein the added mass of the mixed aqueous solution of the sodium citrate, the glycine and the acetic acid is 7 times of the total mass of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp mixed diced meat, performing water bath constant-temperature extraction at 75 +/-5 ℃ for 2 hours, then performing air cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) Placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 30min, cooling to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the moringa oleifera leaf extract of the comparative example.
Comparative example 4
A growth regulator for potatoes comprises benayl aminopurine, choline chloride and a hawthorn extract, and comprises the following components in parts by weight: 10 parts of benzylaminopurine, 4 parts of choline chloride and 7 parts of hawthorn extract.
The preparation method of the hawthorn extract comprises the following steps:
(1) cutting hawthorn pulp into pieces, placing the cut pieces into a container, and adding deionized water into the container, wherein the adding amount of the deionized water is 1.6 times of the total mass of the pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) Preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, wherein in the mixed aqueous solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 2g/100mL, the concentration of glycine is 0.3g/100mL, the concentration of acetic acid is 0.3g/100mL, and the balance is water; adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into the container, wherein the added mass of the mixed aqueous solution of sodium citrate, glycine and acetic acid is 7 times of the total mass of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp mixed diced pieces, extracting for 2 hours in a water bath at the temperature of 75 +/-5 ℃, then cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 30min, cooling to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the hawthorn extract of the comparative example.
Comparative example 5
A growth regulator for potatoes comprises benayurine, choline chloride and a sophora japonica-moringa oleifera leaf-hawthorn fruit composite extract, and the components in parts by weight are as follows: 10 parts of benzylaminopurine, 4 parts of choline chloride and 7 parts of sophora japonica-moringa oleifera leaf-hawthorn fruit composite extract.
The preparation method of the acacia flower-moringa oleifera leaf-hawthorn composite extract comprises the following steps:
(1) mixing the sophora japonica flower petals in a mixing mass ratio: and (3) horseradish tree leaves: mixing sophora japonica flower petals, moringa oleifera leaves and hawthorn pulp in a ratio of 1:0.7:0.2, chopping, putting the chopped diced pieces into a container, and adding deionized water into the container, wherein the addition amount of the deionized water is 1.6 times of the total mass of the diced pieces in the container. Sealing the container, pressurizing to 10 standard atmospheric pressures, standing for 20min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing in a vacuum box, vacuumizing to 0.01 standard atmospheric pressure, maintaining the pressure for 6min, and taking out the container;
(2) adding deionized water into the container, wherein the mass of the deionized water is 7 times of the total mass of the mixed diced pieces of the acacia flower petals, the moringa leaves and the hawthorn fruit pulp, extracting for 2 hours in a water bath at the temperature of 75 +/-5 ℃, then cooling to normal temperature, and filtering to obtain filtrate A and filter residues;
(3) placing the filter residue in a container, adding deionized water into the container, adding deionized water with the mass 5 times of that of the filter residue in the container, sealing the container, heating to 140 +/-5 ℃, keeping the temperature for 30min, cooling to normal temperature, opening the container, and filtering to obtain filtrate B;
(4) And mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/9 of the volume before concentration to obtain the acacia flower-moringa oleifera leaf-hawthorn fruit composite extract of the comparative example.
Example 5
The potato of the variety Favorita is taken as a research object. Designing 10 potato test fields, planting 10 rows and 10 columns of potatoes in each test field, wherein the row spacing and the column spacing are 0.6m, and applying a compound fertilizer of 1200kg/hm -2 The base fertilizer is uniformly supplemented with 150kg/hm of nitrogen fertilizer in the seedling stage -2 . On the third day after the seedling stage, the growth regulators prepared by the methods described in each example and comparative example were applied, respectively, by the following methods: adding water into the corresponding growth regulator to prepare a medicament, wherein the water adding ratio is 150 mg/L. Spraying is carried out in the seedling stage of the potatoes, and the spraying amount is 300mL per plant. Wherein the control group is sprayed with clear water,the boron application rate was also 300 mL/strain. Other field management modes are carried out according to the conventional method, and all the test fields are the same. After harvesting, the yield increase of each test field to which the growth regulator was applied was calculated as (potato yield of the test field-potato yield of the control test field) ÷ potato yield of the control test field × 100%, and the results are shown in table 1. In addition, the starch content of potatoes produced in each test field was determined by taking 5 potatoes produced in the same field and averaging the starch content of the potatoes produced in the test field, and the results are shown in table 1.
TABLE 1
As can be seen from Table 1, the growth regulator of the present invention has a significant growth promoting effect on potatoes, the yield of potatoes is significantly increased after application, and the potato starch content is significantly higher than that of a control group to which the regulator of the present invention is not applied, indicating that the growth regulator of the present invention has the efficacy of improving the yield and quality of potatoes. Comparing example 3 with various proportions, it can be seen that the acacia flower-moringa oleifera leaf-hawthorn fruit composite extract has a good effect of improving the growth regulator, and compared with the extract without the extract or the extract with only a single component, the effect of the composite extract is obviously better, which indicates that the three composite extracts have a synergistic effect of improving the growth regulator.
The technical solutions provided by the present invention are described in detail above, and for those skilled in the art, the ideas according to the embodiments of the present invention may be changed in the specific implementation manners and the application ranges, and in summary, the content of the present description should not be construed as limiting the present invention.
Claims (3)
1. The growth regulator for the potatoes is characterized by comprising benayurine, choline chloride and a sophora japonica-moringa oleifera leaf-hawthorn fruit composite extract, wherein the components in parts by weight are as follows: 10 parts of benzylaminopurine, 2-6 parts of choline chloride and 4-9 parts of acacia flower-moringa leaf-hawthorn composite extract, wherein the preparation method of the acacia flower-moringa leaf-hawthorn composite extract comprises the following steps:
(1) Mixing and chopping sophora japonica petals, moringa oleifera leaves and hawthorn pulp, placing chopped diced meat into a container, adding deionized water into the container, sealing the container, pressurizing to 8-10 standard atmospheric pressures, standing for 10-20 min, balancing the internal and external atmospheric pressures of the container, opening the container, filtering to remove residual water in the container, placing the container into a vacuum box, vacuumizing until the pressure is less than or equal to 0.01 standard atmospheric pressure, maintaining the pressure for 5-6 min, and taking out the container; the mixing mass ratio of the acacia flower petals to the moringa leaves to the hawthorn pulp is as follows: and (3) horseradish tree leaves: hawthorn pulp =1: 0.3-0.8: 0.1-0.2;
(2) preparing a mixed aqueous solution of sodium citrate, glycine and acetic acid, adding the mixed aqueous solution of sodium citrate, glycine and acetic acid into the container, extracting for 2-3 h in a water bath at the temperature of 75 +/-5 ℃ at constant temperature, then cooling to the normal temperature, and filtering to obtain a filtrate A and filter residues; in the mixed water solution of sodium citrate, glycine and acetic acid, the concentration of sodium citrate is 1-3 g/100mL, the concentration of glycine is 0.2-0.4 g/100mL, the concentration of acetic acid is 0.2-0.4 g/100mL, and the balance is water; adding the mixed aqueous solution of the sodium citrate, the glycine and the acetic acid into a container, wherein the mass of the mixed aqueous solution is 7-8 times of the total mass of the mixed diced American sophora flower petals, the moringa leaves and the hawthorn fruit pulp;
(3) Placing the filter residue in a container, adding deionized water into the container, sealing the container, heating to 140 +/-5 ℃, preserving heat for 20-30 min, cooling in air to normal temperature, opening the container, and filtering to obtain filtrate B; wherein the mass of the deionized water added into the container is 5-6 times of the mass of the filter residue in the container;
(4) and mixing the filtrate A and the filtrate B, and concentrating under reduced pressure to 1/10-1/9 of the volume of the filtrate before concentration to obtain the robinia pseudoacacia flower-moringa oleifera leaf-hawthorn fruit compound extract.
2. The method for applying a growth regulator according to claim 1, wherein the growth regulator is mixed with water to prepare a chemical, and the chemical is sprayed at a rate of 200 to 300 mL/plant in a seedling stage of potatoes.
3. The method for applying the growth regulator according to claim 2, wherein the water adding ratio of the growth regulator is growth regulator/water = 120-180 mg/L.
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