CN107568257B - Formula for improving seed setting rate of rice seeds and preparation method and application thereof - Google Patents
Formula for improving seed setting rate of rice seeds and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
Abstract
The invention belongs to the field of rice seeds, and discloses a formula for improving the maturing rate of rice seeds and a preparation method and application thereof. The formula is prepared from the following raw materials in percentage by mass: 70.00-80.00% of ampelopsis grossedentata extract dihydromyricetin, 6.00-10.00% of bitter almond extract, 3.00-5.00% of mulberry root extract, 3.00-5.00% of resveratrol, 2.00-4.00% of vitamin E, 2.00-4.00% of vitamin C, 1.00-3.00% of agricultural streptomycin sulfate and 1.00-3.00% of paclobutrazol. The raw materials are weighed according to the proportion and placed in a blending tank, the mixture is uniformly stirred, and the formula for improving the maturing rate of the rice seeds is obtained after the composite aluminum bag packaging or the canning. The invention has reasonable formula, simple processing and preparation and convenient use, and provides an ideal medicine for improving seed setting rate.
Description
Technical Field
The invention belongs to the field of rice seeds, and particularly relates to a formula for improving the maturing rate of rice seeds and a preparation method and application thereof.
Background
The seed set of rice is the percentage of the number of the filled grains in the total glumes. The maturing rate level can be used as one of important reference standards for evaluating the practical application value of rice varieties and is also an important factor influencing the rice yield. The maturing rate of rice is an important regulating and controlling factor of the rice yield, and the maturing rate of rice varieties directly influences the rice yield (Yang hong, etc. 2013). The fruit set rate is calculated by the following formula:
set percentage (%) -total number of rice ears- × 100
The period with a large influence on the rice setting rate is mainly the pollen development period or the filling period. Sterility, empty grain, or rental grain is prone to occur if the effects of adverse conditions are encountered during these several periods. In addition to genetic control, the setting percentage is susceptible to many environmental factors and is thus greatly changed, making it the most unstable element (leaf plum 2015) among the constituent elements of rice yield traits.
At present, the aspects of improving the seed setting rate of rice mainly comprise the culture and the reasonable fertilization of strong seedlings, a dry-wet alternative irrigation technology and chemical substance (plant growth regulator) regulation, wherein the most important aspect is the chemical substance (plant growth regulator) regulation. If 2.4-D (with the concentration of 2-10ppm), monopotassium phosphate (with the concentration of 0.1-0.2%), boric acid (with the concentration of 0.1%), vitamin G (with the concentration of 50ppm) and triacontanol (with the concentration of 0.05-0.08mg/L) are mixed in the heading and flowering period and sprayed on the leaf surfaces, or 500G of urea, 1000G of calcium superphosphate, 250G of potassium chloride and 50kg of water are mixed, not only can grain grouting be accelerated, but also the aging of functional leaves and root systems can be delayed. Significantly improved fruit set rate and increased yield (winter birth 1982). The exogenous hormone plant growth regulator GA3+6-BA + BR is sprayed in the early rice heading stage and the full heading stage, so that the grain filling rate can be improved, the conversion of cane sugar to starch and the accumulation of starch are promoted, and the maturing rate and the yield are improved. Researches such as Zhao Quanzhi and the like show that spraying of the compound plant growth regulator PR1 in the booting period can obviously promote the initial grouting rate of weak grains, start the grouting of the weak grains as soon as possible, improve the relative fullness of grains, improve the quality of rice, reduce the chalky grain rate and chalky degree of the grains, and increase the polished rice rate and the whole polished rice rate. The methods improve the seed setting rate of the rice to a certain extent, but still have the defects of small improvement amplitude, hormone pollution, high cost and the like.
Disclosure of Invention
In order to solve the defects in the prior art, the invention mainly aims to provide a formula for improving the maturing rate of rice seeds. The formula can be used independently or be combined with the field management measures in the later growth stage of the rice seeds to synergistically improve the maturing rate of the rice seeds.
Another object of the present invention is to provide a process for preparing the above formulation.
The invention also aims to provide application of the formula.
The purpose of the invention is realized by the following technical scheme:
the formula for improving the seed setting rate of the rice seeds is prepared from the following raw materials in percentage by mass:
preferably, the formula is prepared from the following raw materials in percentage by mass:
the ampelopsis grossedentata extract dihydromyricetin is a product obtained by extracting and purifying ampelopsis grossedentata serving as a raw material, wherein the mass percentage content of the dihydromyricetin is more than or equal to 55%;
the mass percentage content of the bitter almond extract is more than or equal to 50.0 percent calculated by amygdalin;
the mulberry root extract contains 1-Deoxynojirimycin (DNJ) with a mass percentage of more than or equal to 0.5%;
the resveratrol is a product obtained by extracting and purifying grape skin, grape seeds or giant knotweed rhizome serving as a raw material, wherein the resveratrol is more than or equal to 20% in percentage by mass;
the mass percentage of the vitamin E in the vitamin E is more than 95 percent;
the mass percentage of the vitamin C in the vitamin C is more than 95 percent
The mass percentage content of the streptomycin sulfate in the agricultural streptomycin sulfate is more than 70 percent
The mass percentage content of the paclobutrazol in the paclobutrazol is more than 70 percent.
The ampelopsis grossedentata extract dihydromyricetin is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting the dry vine tea raw materials by an ethanol extraction method to obtain vine tea extract, concentrating the extract to 30-40% of the original volume, placing the concentrated solution in a refrigeration house at-4 ℃ for standing for 24 hours, and precipitating the bottom of the dry solution to obtain a dihydromyricetin extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%; the Ampelopsis grossedentata is prepared from Ampelopsis grossedentata produced in Guangdong, Guangxi, Hunan, Hubei or Jiangxi.
Preferably, the ethanol is at a concentration of 90% by volume; the sediment at the bottom of the drying liquid is obtained by drying the sediment at the bottom of the concentrated solution in an oven at the temperature of 90-95 ℃; the vine tea is produced in spring and summer in Zhang Jiajie region of Hunan province.
The bitter almond extract is prepared by the following steps: adopting mature bitter almond fruits collected in summer, removing pulp and nucleocapsid, taking out seeds, and drying at 35-45 ℃ to obtain dry bitter almond raw materials; extracting dried semen Armeniacae amarum with ethanol to obtain extractive solution, and spray drying to obtain semen Armeniacae amarum extract; the mass ratio of the dried bitter almond raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 65-95%, and the preferred volume percentage concentration is 70%; the almond fruit is dried mature seed of Prunus armeniaca L.var.ansu Maxim, Prunus sibirica L.Siberian, Prunus mandshurica (Maxim.) Koehne or Prunus armeniaca L.in Rosaceae, preferably Prunus northeast.
The mulberry root extract is prepared by the following steps: drying fresh mulberry roots at the temperature of 35-45 ℃ to obtain a mulberry root drying raw material; extracting the mulberry root dried raw material by adopting an ethanol extraction method to obtain a mulberry root extracting solution, and then performing a spray drying method to obtain a mulberry root extract; the mass ratio of the mulberry root dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 65-95%, and the preferred volume percentage concentration is 70%; the fresh mulberry root is produced in Guangdong mulberry (Yue Mulberry) in winter.
The resveratrol is prepared by the following steps: extracting rhizoma Polygoni Cuspidati as raw material with 75% ethanol 6 times the volume of the raw material as extraction solvent at 70-75 deg.C for 1 hr, concentrating, recovering ethanol, and spray drying the extractive solution to obtain resveratrol extract.
The vitamin E is produced by Zhengzhou Longsheng food chemical industry limited company; the vitamin C is produced by Zhengzhou Chengwang chemical industry Co., Ltd; the agricultural streptomycin sulfate and paclobutrazol are produced by Sichuan Chengdu general bioengineering company Limited.
The preparation method of the formula for improving the maturing rate of the rice seeds comprises the following operation steps: weighing Ampelopsis grossedentata extract dihydromyricetin, bitter almond extract, mulberry root extract, resveratrol, vitamin E, vitamin C, agricultural streptomycin sulfate and paclobutrazol according to the proportion, placing the mixture into a blending tank, uniformly stirring, and packaging or canning by using a composite aluminum bag to obtain the formula for improving the maturing rate of the rice seeds.
The formula is applied to improving the seed setting rate of rice seeds.
The application comprises the following operation steps: in the pollen development period, the flowering period and/or the filling period of the rice seed production, the formula for improving the seed setting rate of the rice seeds is stirred and dissolved by water to form direct use liquid with the mass percentage of 0.5-1.0%, the direct use liquid is sprayed on rice leaves in a rice seed production field according to the use amount of 50-60 kg/mu, and the direct use liquid is preferably sprayed for 1 time in the pollen development period, the flowering period and the filling period of the rice seeds respectively.
The principle of the invention is as follows:
the ampelopsis grossedentata extract dihydromyricetin is a main active component in ampelopsis grossedentata plants, and has multiple functions of resisting aging, resisting oxidation and the like. The dihydromyricetin in the formula has the main functions of eliminating active oxygen and preventing the generation and accumulation of malondialdehyde, so that the aim of improving the pollen activity and further improving the seed setting rate is fulfilled.
Bitter almond extract: the main component is amygdalin which is vitamin B17, is one of the effective components in traditional Chinese medicine amygdalin and widely exists in seeds of rosaceous plants such as peaches, apricots, plums, apples and hawthorns. In the formula, the dihydromyricetin extracted from the vine tea and the vine tea are mainly utilized to have a synergistic antioxidation effect.
Mulberry root extract: the main component is a alkaloid compound, and the traditional medicine considers that the traditional Chinese medicine has the effects of treating fright epilepsy, conjunctival congestion, toothache, arthralgia and myalgia and the like. The formula mainly balances the nutrition growth and reproductive growth of the rice, thereby achieving the purpose of improving the seed setting rate.
Resveratrol: resveratrol is a phytoncide, and has anticancer, blood pressure lowering, and osteoporosis preventing effects. It also has effects in treating inflammation, relieving lipid metabolism disorder, and resisting oxidation. Resveratrol is also an activator of histone deacetylase and has effect in inhibiting cell aging. The sterilization effect of the formula is mainly utilized in the formula, so that the damage of microorganisms to rice in the pollination process is reduced, the premature senility of cells is prevented, and the seed setting rate is improved.
Vitamin e (vitamin e) is a fat-soluble vitamin whose hydrolysate is tocopherol, one of the most important antioxidants. The formula is mainly used for facilitating the lipid to enter a lipid cell membrane, participate in lipid metabolism of embryonic cells and eliminate series of free radicals in the metabolic process, so that the aims of protecting the embryonic cells and improving the seed setting rate are fulfilled.
Vitamin C: is a water-soluble antioxidant. The formula is mainly used for enhancing the anti-stress capability and the immunity of the embryo cells of the seeds to the external environment, thereby improving the seed setting rate.
Agricultural streptomycin sulfate: is easily soluble in water, and is an antibiotic bactericide. The main control objects are bacterial brown spot and bacterial rot, and the pesticide is a common agricultural pesticide. The sterilization effect is mainly utilized in the formula to kill microorganisms inside and outside the glumes of the seeds, so that the damage of the microorganisms to the seeds in the storage and seed soaking processes is reduced, and the seed setting rate is improved.
Paclobutrazol: acts synergistically with streptomycin sulphate for agricultural use.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the invention has reasonable formula, comprehensively utilizes the respective effects of chemical raw materials and extracts in natural plants on improving the seed setting rate, and has very obvious effect on improving the seed setting rate through experimental verification;
(2) simple processing and manufacturing, convenient use and provides an ideal medicine for improving seed setting rate.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
The following examples use the following sources of raw materials:
1. the ampelopsis grossedentata extract dihydromyricetin is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting the dry vine tea raw materials by an ethanol extraction method to obtain vine tea extract, concentrating the extract to 30-40% of the original volume, placing the concentrated solution in a refrigeration house at-4 ℃ for standing for 24 hours, and precipitating the bottom of the dry solution to obtain a dihydromyricetin extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%, and the preferred volume percentage concentration is 90%. The ampelopsis grossedentata adopts ampelopsis grossedentata produced in Guangdong, Guangxi, Hunan, Hubei, Jiangxi and the like, and preferably is ampelopsis grossedentata produced in spring and summer in Zhang Jiajie region of Hunan province. And the sediment at the bottom of the drying liquid is obtained by drying the sediment at the bottom of the concentrated solution in an oven at the temperature of 90-95 ℃. In the obtained dihydromyricetin extract product, the mass percentage content of dihydromyricetin is more than or equal to 55%.
2. The bitter almond extract is prepared by the following steps: adopting a mature bitter almond fruit collected in summer (the bitter almond fruit is dried mature seeds of wild apricot, Siberian apricot, northeast apricot or apricot of Rosaceae), removing pulp and nucleocapsid, taking out seeds, and drying at 35-45 deg.C to obtain dried bitter almond; extracting dried semen Armeniacae amarum with ethanol to obtain extractive solution, and spray drying to obtain semen Armeniacae amarum extract; the ethanol concentration used for ethanol extraction is 70-80%, and the mass ratio of the raw materials to the ethanol is 1: 10-12, the leaching temperature is 80-85 ℃, and the leaching time is 1 hour. The obtained semen Armeniacae amarum extract contains at least 50.0 wt% of amygdalin.
3. The mulberry root extract is prepared by the following steps: drying fresh mulberry roots at the temperature of 35-45 ℃ to obtain a mulberry root drying raw material; extracting the mulberry root dried raw material by adopting an ethanol extraction method to obtain a mulberry root extracting solution, and then performing a spray drying method to obtain a mulberry root extract; the mass ratio of the mulberry root dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%. The obtained mulberry root extract contains 1-Deoxynojirimycin (DNJ) with a mass percentage of more than or equal to 0.5%.
4. The resveratrol extract is prepared by the following steps: rhizoma Polygoni Cuspidati rich in resveratrol is used as raw material, ethanol with volume percentage of 75% 6 times of the raw material is added as extraction solvent, the extraction is carried out at 70-75 deg.C for 1 hr, ethanol is recovered by concentration, and the extract is spray dried to obtain resveratrol extract. The resveratrol extract contains resveratrol more than 20 wt%.
5. The vitamin E is produced by Zhengzhou Longsheng food chemical industry limited company, and the mass percentage content is more than 95 percent.
6. The vitamin C is produced by Zhengzhou Chengwang chemical industry Co., Ltd, and the mass percentage content is more than 95%.
7. Agricultural streptomycin sulfate and paclobutrazol are produced by Sichuan Chengdu general bioengineering company Limited, the mass percentage content of the streptomycin sulfate is 72 percent, and the mass percentage content of the paclobutrazol is 75 percent.
Example 1:
74.00% of ampelopsis grossedentata extract dihydromyricetin, 8.00% of bitter apricot seed extract, 4.00% of mulberry root extract, 4.00% of resveratrol, 3.00% of vitamin E, 3.00% of vitamin C, 2.00% of agricultural streptomycin sulfate and 2.00% of paclobutrazol 8 raw materials are respectively weighed and placed in a blending tank, uniformly stirred, and packaged or canned by a composite aluminum bag to obtain the formula product for improving seed setting rate.
Example 2:
respectively weighing 75.00 mass percent of ampelopsis grossedentata extract dihydromyricetin, 7.00 mass percent of bitter apricot seed extract, 5.00 mass percent of mulberry root extract, 4.00 mass percent of resveratrol, 2.00 mass percent of vitamin E, 3.00 mass percent of vitamin C, 2.00 mass percent of agricultural streptomycin sulfate and 2.00 mass percent of paclobutrazol 8 raw materials, placing the raw materials into a blending tank, uniformly stirring, and packaging or canning by using a composite aluminum bag to obtain a formula product for improving seed setting rate.
Example 3: variation of seed production seed setting rate after spraying formula product for improving setting rate during seed production of hybrid rice
Weighing 500g of the product prepared in the example 1, stirring and dissolving the product into emulsion by using 50kg of water, and filling the emulsion into a plastic barrel to obtain the direct use solution of the formula product for improving the seed setting rate, wherein the mass percent of the direct use solution is 1%. The direct liquid is sprayed for 1 time respectively in the pollen development period and the late stage of milk maturity in the field for seed production of hybrid rice seeds of 8-best-8-best and 368-day-best, and the spraying dosage is 50 kg/mu per time. In addition, a control CK without spraying the direct solution is arranged in the adjacent zone of the field for producing the hybrid rice seeds sprayed with the direct solution. After the seeds are mature, the seed setting rate is measured.
Set percentage (%) -total number of rice ears- × 100
The following table 1 shows the data change of the seed setting rate after the treatment during the 2 hybrid rice combined seed production periods (the hybrid rice combined material is provided by the Jincano rice seed industry Co., Ltd. in Guangdong province), and it can be seen that the seed production setting rate of the seeds sprayed with the product of the invention is obviously improved.
Table 1 shows the variation of seed setting rate of different "368" series hybrid rice after being treated by the formula product during seed production
Example 4: the change situation of the rice setting rate after the formula product for improving the setting rate is sprayed during the field production of the 998 series hybrid rice
Weighing 0.75kg of the product prepared in the embodiment 2, stirring and dissolving the product by using 100kg of water to form emulsion, and filling the emulsion by using a plastic barrel to obtain the direct liquid for the formula product for improving the seed setting rate, wherein the mass percent of the direct liquid is 0.75%. The direct solution is sprayed on the experimental fields for producing hybrid rice seeds of Tianyou 998, Qiuyou 998 and II you 998 for 1 time respectively in the late stage of maturity of pollen in the development period and the filling period. The area of each experimental field is 0.1 mu, and the application amount of each spraying is 60 kg/mu. In addition, a control CK without spraying the direct solution is arranged in the adjacent zone of the field for producing the hybrid rice seeds sprayed with the direct solution. After the seeds are mature, the seed setting rate is measured.
Set percentage (%) -total number of rice ears- × 100
The following table 2 shows the data change of the setting percentage of 2 '998' series hybrid rice combinations (hybrid rice combination materials are provided by Jincano Rice variety Co., Ltd. in Guangdong province), and it can be seen that the seed setting percentage of the product of the invention sprayed on the seed is obviously improved.
TABLE 2 variation of setting percentage after treatment with the formulation during field production of "998" series hybrid rice
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A composition for increasing seed set percentage of rice seeds, which is characterized in that: the composition is prepared from the following raw materials in percentage by mass:
2. the composition for increasing seed set percentage of rice as claimed in claim 1, wherein: the composition is prepared from the following raw materials in percentage by mass:
3. the composition for increasing seed set percentage of rice as claimed in claim 1, wherein: the ampelopsis grossedentata extract dihydromyricetin is a product obtained by extracting and purifying ampelopsis grossedentata serving as a raw material, wherein the mass percentage content of the dihydromyricetin is more than or equal to 55%;
the mass percentage content of the bitter almond extract is more than or equal to 50.0 percent calculated by amygdalin;
the mulberry root extract contains 1-Deoxynojirimycin (DNJ) with a mass percentage of more than or equal to 0.5%;
the resveratrol is a product obtained by extracting and purifying grape skin, grape seeds or giant knotweed rhizome serving as a raw material, wherein the resveratrol is more than or equal to 20% in percentage by mass;
the mass percentage of the vitamin E in the vitamin E is more than 95 percent;
the mass percentage of the vitamin C in the vitamin C is more than 95 percent;
the mass percentage content of the streptomycin sulfate in the agricultural streptomycin sulfate is more than 70 percent;
the mass percentage content of the paclobutrazol in the paclobutrazol is more than 70 percent.
4. The composition for increasing seed set percentage of rice as claimed in claim 1, wherein:
the ampelopsis grossedentata extract dihydromyricetin is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting the dry vine tea raw materials by an ethanol extraction method to obtain vine tea extract, concentrating the extract to 30-40% of the original volume, placing the concentrated solution in a refrigeration house at-4 ℃ for standing for 24 hours, and precipitating the bottom of the dry solution to obtain a dihydromyricetin extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%; the Ampelopsis grossedentata is prepared from Ampelopsis grossedentata produced in Guangdong, Guangxi, Hunan, Hubei or Jiangxi.
5. The composition for increasing seed set percentage of rice as claimed in claim 4, wherein: the volume percentage concentration of the ethanol is 90%; the sediment at the bottom of the drying liquid is obtained by drying the sediment at the bottom of the concentrated solution in an oven at the temperature of 90-95 ℃; the vine tea is produced in spring and summer in Zhang Jiajie region of Hunan province.
6. The composition for increasing seed set percentage of rice as claimed in claim 1, wherein:
the bitter almond extract is prepared by the following steps: adopting mature bitter almond fruits collected in summer, removing pulp and nucleocapsid, taking out seeds, and drying at 35-45 ℃ to obtain dry bitter almond raw materials; extracting dried semen Armeniacae amarum with ethanol to obtain extractive solution, and spray drying to obtain semen Armeniacae amarum extract; the mass ratio of the dried bitter almond raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 65-95%;
the mulberry root extract is prepared by the following steps: drying fresh mulberry roots at the temperature of 35-45 ℃ to obtain a mulberry root drying raw material; extracting the mulberry root dried raw material by adopting an ethanol extraction method to obtain a mulberry root extracting solution, and then performing a spray drying method to obtain a mulberry root extract; the mass ratio of the mulberry root dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 65-95%;
the resveratrol is prepared by the following steps: extracting rhizoma Polygoni Cuspidati as raw material with 75% ethanol 6 times the volume of the raw material as extraction solvent at 70-75 deg.C for 1 hr, concentrating, recovering ethanol, and spray drying the extractive solution to obtain resveratrol extract.
7. The method for preparing the composition for improving the seed setting percentage of rice according to claim 1, comprising the following steps: weighing Ampelopsis grossedentata extract dihydromyricetin, bitter almond extract, mulberry root extract, resveratrol, vitamin E, vitamin C, agricultural streptomycin sulfate and paclobutrazol according to the proportion, placing the mixture into a blending tank, uniformly stirring, and packaging or canning by using a composite aluminum bag to obtain the composition for improving the seed setting rate of the rice.
8. The use of the composition for increasing seed set percentage of rice according to any one of claims 1 to 6, wherein the composition comprises: the application comprises the following operation steps: in the development period, the flowering period and/or the filling period of the pollen produced by the rice seeds, the composition for improving the seed setting rate of the rice seeds is stirred and dissolved by water to form direct solution with the mass percentage of 0.5-1.0%, and the direct solution is sprayed on the rice leaves in the field for producing the rice seeds according to the dosage of 50-60 kg/mu.
9. Use according to claim 8, characterized in that: the application is that the spraying is carried out for 1 time respectively in the pollen development period, the flowering period and the filling period of rice seeds.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710711525.XA CN107568257B (en) | 2017-08-18 | 2017-08-18 | Formula for improving seed setting rate of rice seeds and preparation method and application thereof |
| PCT/CN2018/098450 WO2019033933A1 (en) | 2017-08-18 | 2018-08-03 | Formulation for increasing seed setting rate of rice, preparation method therefor and use thereof |
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| CN201710711525.XA CN107568257B (en) | 2017-08-18 | 2017-08-18 | Formula for improving seed setting rate of rice seeds and preparation method and application thereof |
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| CN107568257B true CN107568257B (en) | 2020-06-26 |
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| CN107568257B (en) * | 2017-08-18 | 2020-06-26 | 广东省农业科学院农业生物基因研究中心 | Formula for improving seed setting rate of rice seeds and preparation method and application thereof |
| CN109287482B (en) * | 2018-10-30 | 2022-03-01 | 江西智汇中药材种植有限公司 | Special culture medium for tissue culture and rapid propagation of gardenia jasminoides |
| CN114557352B (en) * | 2022-03-09 | 2024-02-02 | 广东省农业科学院农业生物基因研究中心 | Composition for promoting early harvesting of rice seeds as well as preparation method and application thereof |
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| CN1049771A (en) * | 1989-09-01 | 1991-03-13 | 帝国化学工业公司 | Composition pesticide |
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| CN106106548A (en) * | 2016-06-28 | 2016-11-16 | 广东省农业科学院水稻研究所 | A kind of composite water spike of rice sprouts inhibitor and its preparation method and application |
| CN106172523A (en) * | 2016-06-28 | 2016-12-07 | 广东省农业科学院水稻研究所 | A kind of Green Water spike of rice sprouts inhibitor and its preparation method and application |
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| CN107568257B (en) * | 2017-08-18 | 2020-06-26 | 广东省农业科学院农业生物基因研究中心 | Formula for improving seed setting rate of rice seeds and preparation method and application thereof |
| CN107517995B (en) * | 2017-08-18 | 2020-09-22 | 广东省农业科学院农业生物基因研究中心 | Formula for improving storage capacity of hybrid rice seeds, preparation method and application |
| CN107517994B (en) * | 2017-08-18 | 2020-09-22 | 广东省农业科学院农业生物基因研究中心 | Formula for improving rice grain filling degree and preparation method and application thereof |
-
2017
- 2017-08-18 CN CN201710711525.XA patent/CN107568257B/en active Active
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| CN1049771A (en) * | 1989-09-01 | 1991-03-13 | 帝国化学工业公司 | Composition pesticide |
| CN103250695A (en) * | 2013-05-24 | 2013-08-21 | 广东省农业科学院水稻研究所 | Natural plant extract rice seed embryo vigor retention agent as well as preparation method and application thereof |
| CN103250744A (en) * | 2013-05-24 | 2013-08-21 | 广东省农业科学院水稻研究所 | Composite natural plant extract rice seed initiator as well as preparation method and application thereof |
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| WO2019033933A1 (en) | 2019-02-21 |
| CN107568257A (en) | 2018-01-12 |
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