CN106106548A - A kind of composite water spike of rice sprouts inhibitor and its preparation method and application - Google Patents
A kind of composite water spike of rice sprouts inhibitor and its preparation method and application Download PDFInfo
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Abstract
The invention belongs to Rice Panicle and sprout inhibitor field, disclose a kind of composite water spike of rice and sprout inhibitor and its preparation method and application.This Rice Panicle is sprouted inhibitor and is made up of following raw material by mass percentage: Coumarins extract 17.00~18.00%, Semen Armeniacae Amarum extract 15.00~16.00%, Radix Mori extract 10.00~11.00%, salicylic acid extract 15.00~16.00%, plum extract 14.50~16.00%, Fructus Myricae rubrae extract 20.00~22.50%, paclobutrazol 3.00~3.50%, abscisic acid 1.50~2.00%.Inventive formulation is reasonable, experiments verify that and Rice Panicle is sprouted inhibition clearly, the purpose having reached the most safely but also having prevented fringe from sprouting;Processing and fabricating is simple, easy to use, sprouts for prevention of water spike of rice and provides a kind of preferably medicine.
Description
Technical field
The invention belongs to Rice Panicle and sprout inhibitor field, sprout inhibitor and preparation thereof particularly to a kind of composite water spike of rice
Methods and applications.
Background technology
Rice Panicle sprouts the phenomenon before referring to the rice paddy seed maturation later stage and not yet departing from maternal plant at head sprouting.Oryza sativa L. makees
For one of cereal crops that China is important, the quality of its yield and quality is the problem of relation its people's lifeblood.China is mostly
District, all there will be fringe in various degree during Oryza sativa L. grain-production, the hybrid paddy rice production of hybrid seeds and breeding and sprouts.South China is because of by the summer
Autumn season long wet weather climatic effect, the paddy fields having more than 6% occurs that fringe sprouts phenomenon, and wherein Cross-incompatibility groups district fringe is sprouted
Rate can reach 20%;Particularly during breeding of hybrid rice, due to a large amount of uses of " 920 ", add sterile line
Specific dormant trait is shallow and seed easily splits the feature of grain husk, and fringe sprouts rate up to 60%, may be up to more than 95% (Wang Qiwei time serious
1992).For the seed occurring fringe to sprout, substantially lose Seed practical value, and some is not yet occurred to fringe bud performance is planted
Strain, often has occurred and that various metabolic alterations, sprouts for fringe and prepare inside seed, be easier to split during the storage of seeds
Becoming, seed vitality declines, and also can reduce the nutrition of seed, sowing and processing quality simultaneously.
Numerous studies confirm, it is kind inherited character and the coefficient result of the external environment factor that Rice Panicle is sprouted.Kind
Inherited character include the inherited characters such as seed and the character in fringe portion, the thickness of grain husk shell and kernel seed coat colour all to the dormancy of seed and
Fringe sprouts the impact having in various degree;The external environment factor includes the hydrolase in seed moisture content and temperature and seed body
Content, phytohormone (gibberellins GA and abscisic acid ABA) content directly affect Rice Panicle and sprout.
In the case of breeding methods and common cultivation step all can not preferably suppress fringe to sprout, applied chemistry reagent control
Making this phenomenon is one of measure most-often used during current agricultural produces.There are some researches prove, sprout before death (one at Rice Panicle
As in dough stage) spray certain density ABA and can effectively prevent fringe from sprouting, but owing to ABA price is high, when large area uses,
Bring pressure to production cost, and the seed that ABA processed has stronger hibernation feature, needs the deposition through the long period
Process and could sprout (Gubler etc. 2005;Tan Huijuan etc. 2006).Paclobutrazol and uniconazole P are the synthesis of plant endogenous gibberellins
Inhibitor, they are possible not only to preferably suppress Rice Panicle to sprout, moreover it is possible to increase setting percentage and yield to a certain extent
(Rademacher 1991).There are some researches show, spray the paclobutrazol of 750g Rise's boot period every mu, fringe can be sprouted incidence rate
Reducing by 70%, in the range of finite concentration, along with the increase inhibition of concentration the best (Liao Yong auspicious 2009), but large area uses
After whether both growth substances bring pedo relict and suppression to agricultural production, the side effect such as work still needs to be verified further.Blue or green
Fresh element (maleic acid hydrazide, MH) and daminozide (B9) have the effect that preferably suppression Rice Panicle is sprouted, and this material is by reducing seed
Activity, has containment, injury effect to germination, there are some researches show through the spike of rice that MH processed, the Repiration of seed and interior
The content of source gibberellins has declined, but research shows that MH and B9 can be distorted by induced chromosome, has carcinogenesis, therefore
(Wang Yu unit 1989 is the most gradually eliminated on producing;Hu Weimin 2000;Tao etc. 2007).Additionally at present commercialization " fringe is sprouted
Inhibitor " (being mainly composed of 30% maleic hydrazide+60% retardation organic acid) and " Suiyake " (main component is 1g/L coumaric acid
With 0.03g/L paclobutrazol) there are a certain degree of application (what is said or talked about Hui Juan etc. 2006 aborning;Zhang Chaoxian and height elder generation light 2009).Zhejiang
Jiang great Xue Hu Jin (2014) finds, the eugenol spraying 1-2g/kg at Oryza sativa L. milking maturity latter stage to the initial stage stage of yellow ripeness can effectively suppress
Fringe is sprouted, the method environmentally safe.
The research of summary, when breeding methods and common cultivation step all can not preferably suppress the situation that fringe sprouts
Under, it should it is one of measure most-often used during current agricultural produces that chemical raw material controls this phenomenon.But due to chemical principle
Material and growth hormone the impact of the envirment factors such as soil, air, water body and seed vitality and toxicant is needed into
One step is inquired into, and there is safety issue.Therefore, reduce chemical raw material and the usage amount of growth hormone, with in natural plants as far as possible
It is appropriate that the effective ingredient part replacement chemical raw material extracted and growth hormone form compound fringe to be sprouted inhibitor.
Summary of the invention
In place of solving above-mentioned the deficiencies in the prior art, the primary and foremost purpose of the present invention is to provide one compound
Type Rice Panicle sprouts inhibitor.This fringe sprouts inhibitor can be by being used alone or by the field management with the Oryza sativa L. maturation later stage
Measure combines and controls the fringe sprouting life of Oryza sativa L..
Another object of the present invention is to the preparation method providing above-mentioned compound fringe to sprout inhibitor.
It is still another object of the present invention to provide above-mentioned compound fringe and sprout the application of inhibitor.
The purpose of the present invention is realized by following technical proposals: a kind of composite water spike of rice sprouts inhibitor, this Rice Panicle
Sprout inhibitor to be made up of following raw material by mass percentage:
This mixed type Rice Panicle is sprouted inhibitor and is preferably made up of following raw material by mass percentage:
In described Coumarins extract, in terms of coumarin, weight/mass percentage composition >=10%;
In described Semen Armeniacae Amarum extract, in terms of amygdaloside, weight/mass percentage composition >=50.0%;
In described Radix Mori extract, count with 1-DNJ (1-Deoxynojirimycin, DNJ), percent mass
Content >=0.5%;
In described salicylic acid extract, in terms of salicylic acid, weight/mass percentage composition >=50%;
In described plum extract, with citrometer, weight/mass percentage composition >=2.5%;
In described Fructus Myricae rubrae extract, with ampelopsin and citrometer, weight/mass percentage composition >=15% of ampelopsin, citric acid
Weight/mass percentage composition >=2.5%;
The purity of described paclobutrazol is more than 15%;
The purity of described abscisic acid is more than 85%;
Described Coumarins extract is to prepare according to the following steps: utilize coumarin kind compound to dissolve in heat alkali liquid
In, the characteristic that acid adding separates out again is extracted.Specifically according to following steps: the raw material of coumarin kind compound rich content is used
Mass percentage concentration 0.5% sodium hydrate aqueous solution heating extraction, removes impurity with ether after extracting solution cooling, then acid adding again
Regulation pH, to neutral, is acidified after concentration again, and Coumarins and glycosides thereof can separate out, and is dried and separates out crystallization Coumarins extract;
The raw material of described coumarin kind compound rich content is Melilotus suaveolens Ledeb., Radix Peucedani, Herba Cichorii, the Radix Angelicae Dahuricae, Fructus Psoraleae, tonka beans or Cymbidium ensifolium (L.) Sw.;
Described raw material is 1:10~12 with the mass ratio of sodium hydrate aqueous solution.
Described Semen Armeniacae Amarum extract is to prepare according to the following steps: uses the ripe Semen Armeniacae Amarum fruit gathered summer, removes
Remove sarcocarp and nucleocapsid, take out seed, under the conditions of temperature 35~45 DEG C, be dried to obtain dry Semen Armeniacae Amarum raw material;By dry Semen Armeniacae Amarum
Raw material uses ethanol extraction method to obtain extracting solution, more spray-dried method obtains Semen Armeniacae Amarum extract;In described ethanol extraction method
The mass ratio being dried Semen Armeniacae Amarum raw material and ethanol is 1:10~12, and the concentration of volume percent of ethanol is 65~95%, preferably
Concentration of volume percent 70%;Described Semen Armeniacae Amarum fruit is to use rosaceous plant Prunus armeniaca L.var.ansu Maxim. (bitter Fructus Pruni) Prunus armeniaca
L.var.ansu Maxim., Siberia Fructus Pruni (Prunus armeniaca L.var.ansu Maxim.) Prunus sibirica L., prunus mandshuricaKoehne Prunus mandshurica
(Maxim.) Koehne or the dry mature seed of Fructus Pruni Prunus armeniaca L., preferably prunus mandshuricaKoehne.
Described Radix Mori extract is to prepare according to the following steps: be dried under the conditions of temperature 35~45 DEG C by fresh Radix Mori
After obtain Radix Mori and be dried raw material;Radix Mori is dried raw material uses ethanol extraction method to obtain Radix Mori extracting solution, more spray-dried method
Obtain Radix Mori extract;In described ethanol extraction method, Radix Mori is dried the mass ratio of raw material and ethanol is 1:10~12, ethanol
Concentration of volume percent is 65~95%, preferred volume percent concentration 70%;Described fresh Radix Mori is to use mulberry (Guangdong, Guangdong
Mulberry) winter produce fresh Radix Mori.
Described salicylic acid extract is to prepare according to the following steps: raw material abundant for salicylic acid content is torn into fritter it
After be immersed in water and be heated to boiling, maintains 20~30 minutes, filtration;Filtrate heating being concentrated into bottom solution has white brilliant
Heating is stopped when body produces;Then lowering the temperature to solution with frozen water, separate out crystal, the crystal being filtrated to get is salicylic acid and extracts
Thing;The raw material that described salicylic acid content enriches is willow, willow, Ilicis Purpureae bark or kiwifruit fruit, preferably bark of willow.
Described plum extract is to prepare according to the following steps: fresh Armeniaca mume Sieb. employing ethanol extraction method is obtained Armeniaca mume Sieb. and carries
Take liquid, more spray-dried method obtains plum extract;In described ethanol extraction method, Armeniaca mume Sieb. raw material with the mass ratio of ethanol is
1:10~12, the concentration of volume percent of ethanol is 65~95%, preferred volume percent concentration 70%;Described Armeniaca mume Sieb. is Guangdong
The Armeniaca mume Sieb. produced.
Described Fructus Myricae rubrae extract is to prepare according to the following steps: the fruit of new arbutus, bark or leaf are used ethanol
Extraction method obtains Fructus Myricae rubrae extracting solution, more spray-dried method obtains Fructus Myricae rubrae extract;In described ethanol extraction method Fructus Myricae rubrae raw material with
The mass ratio of ethanol is 1:10~12, and the concentration of volume percent of ethanol is 65~95%, preferred volume percent concentration
70%;Described Fructus Myricae rubrae is the Fructus Myricae rubrae fruit tree of Zhejiang cultivation.
Above-mentioned mixed type Rice Panicle sprouts the preparation method of inhibitor, including following operating procedure: weigh institute by said ratio
Having raw material to be placed in blend tank, stir, compositing aluminium bag i.e. obtains composite water spike of rice after packing or being canned and sprouts inhibitor.
The principle of the present invention is:
Coumarins extract: main active is coumarin kind compound, its medicine effect is factor anticoagulant
Synthesis at liver.Coumarins medicine is similar to the structure of vitamin K.Coumarins medicine is at liver and vitamin K epoxy
Compound reductase combines, and suppression vitamin K is converted to hydroquinone type by epoxide, and the circulation of vitamin K is suppressed.It may be said that it is fragrant
Legumin class medicine is vitamin K antagon, or competitive inhibitor.This formula uses and suppresses water mainly by it
The synthesis of α-amylase in rice later stage Rice During Ripening Process, reduces soluble sugar content in Oryza sativa L., reduces Repiration, supports with this
The generation that fringe processed is sprouted.
Semen Armeniacae Amarum extract: main component is amygdaloside, amygdaloside is a kind of laetrile, bitter for Chinese medicine
One of effective ingredient in Semen Armeniacae Amarum, is widely present in the seed of the rosaceous plants such as Fructus Persicae, Fructus Pruni, prunus mume (sieb.) sieb.et zucc., Fructus Mali pumilae, Fructus Crataegi, tradition doctor
Show, amygdaloside have antitussive, relieving asthma, antiinflammatory, analgesia and antitumor action, but in this formula mainly by it be
Suppression gibberellins (GA) synthesis, plays the effect of the suppression seed germination of similar abscisic acid (ABA).
Radix Mori extract: be mainly composed of alkaloid compound, traditional medicine thinks have treatment infantile convulsion, conjunctival congestion, tooth
The effect such as pain and bones and muscles pain, but in this formula, mainly suppression rice paddy seed is in the increase entering ripe later stage α-amylase
Reduce soluble sugar in seed, reduce Repiration, thus stop fringe to sprout phenomenon and occur.
Salicylic acid extract: be mainly composed of salicylic acid.Salicylic acid is a kind of phenols hormone, and the growth of scalable plant is sent out
Educate, photosynthesis, the transpiration of plant is also had regulation effect with the absorption of ion with transport.Salicylic acid can also lure simultaneously
Lead the differentiation of plant cell and the generation of chloroplast.Pathogen is also resisted by salicylic acid as interior raw signal involved in plant, logical
Cross induced tissue and produce pathogenesis-related proteins, produce resistance when a part for plant is by pathogenic infection in other parts.
By forming volatile methyl salicylate, this signal also can transmit between different plants.
Plum extract: main active is the organic acid such as citric acid, malic acid and multivitamin and mineral, Huang
The materials such as ketoside, triterpene saponin, polyphenol and alkene, have promoting the production of body fluid to quench thirst, appetite stimulator, disinfecting detoxifying, purification blood, strengthen liver
The effects such as dirty function, prophylaxis of hypertension and cerebral hemorrhage and suppression kinds of tumors.Pharmacological action is antibacterial, anthelmintic, antiallergic, significantly
Improve the effects such as the intestines and stomach, conditioning intestinal merit, free radical resisting, antioxidation, slow down aging.This formula suppresses mainly by it
Gibberellins (GA) synthesizes, and reduces Repiration, plays the effect of the suppression seed germination of similar abscisic acid (ABA).
Fructus Myricae rubrae extract: Myruca ceas red bayberry spp.ing plant Fructus Myricae rubrae leaves, skin, root and the extract of fruit;Main active
For the flavone such as ampelopsin, tannin and polyphenols and the organic acid such as citric acid, malic acid, Fructus Myricae rubrae extract has convergence
The effect of agent, analeptic and emetic, be used for suffering from diarrhoea, jaundice type hepatitis, lymphoid tuberculosis, unrestrained property pharyngolaryngitis etc..The bark element of Fructus Myricae rubrae
Also there is non-oxidizability, eliminate interior free yl.Among the people always with regard to useful Fructus Myricae rubrae antiinflammatory and the tradition for the treatment of toothache.At this formula
In mainly by the hydrolysis of saccharide compound during its suppression seed germination, the effect such as breeding of microorganism.
Paclobutrazol is the triazole type plant growth regulator succeeded in developing the eighties, is the suppression of endogenous gibberellins synthesis
Agent.The agriculture using value of paclobutrazol is its control effect to plant growth.Having and delay plant growing, suppression cane is stretched
Long, shorten internode, promote plant tillering, promotion bud differentiation, increase plant stress-resistance performance, improve the effects such as yield.Originally joining
Side uses and delays plant growing mainly by it, hinder material synthesis and metabolic pathway when sprouting, thus stop seed to be sprouted
Send out.
Abscisic acid (abscisic acid, ABA) is called: natural.The phytohormone of a kind of Developing restraint, because promoting leaf
Son comes off and gains the name.Bud can be made to enter resting state, promote white potato shaped one-tenth tuber etc..Prolongation to cell also has suppression to make
With.This formula uses and promotes that seed enters resting state, Inhibiting germination mainly by it.
Hinge structure of the present invention, has such advantages as and beneficial effect: (1) inventive formulation is reasonable, comprehensive utilization
With extract in chemical raw material, growth hormone and natural plants, fringe is sprouted the respective effect of suppression, makes again chemical raw material, growth
The fringe of hormone preparation is sprouted the side effect of inhibitor and is reduced, and experiments verify that and Rice Panicle is sprouted inhibition clearly, reach
Purpose that is the safest but also that prevent fringe from sprouting;(2) processing and fabricating is simple, easy to use, sprouts for prevention of water spike of rice and provides a kind of ideal
Medicine.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Each raw material sources that following example are used are as follows:
1, Coumarins extract is to prepare according to the following steps: use Melilotus suaveolens Ledeb., Radix Peucedani, Herba Cichorii, the Radix Angelicae Dahuricae, Psoralen
The raw material that fat, tonka beans or Cymbidium ensifolium (L.) Sw. etc. are enriched containing coumarin kind compound, utilizes coumarin kind compound to dissolve in heat alkali liquid,
The characteristic that acid adding separates out again is extracted.During actual extracting, carry with the heating of mass percent concentration 0.5% sodium hydrate aqueous solution
Take, after extracting solution cooling, remove impurity with ether again, then add acid for adjusting pH to neutral, concentrate, then be acidified, then Coumarins and
Its glycosides can separate out, and is dried and separates out crystallization Coumarins extract.The described raw material abundant containing coumarin kind compound and hydrogen-oxygen
The mass ratio changing sodium water solution is 1:10~12.In gained Coumarins extract, in terms of total coumarins, weight/mass percentage composition
>=10%.
2, Semen Armeniacae Amarum extract is to prepare according to the following steps: use ripe Semen Armeniacae Amarum fruit (the bitter Fructus Pruni gathered summer
A kind of fruit, such as apple, pear, etc. is to use rosaceous plant Prunus armeniaca L.var.ansu Maxim., Siberia Fructus Pruni, prunus mandshuricaKoehne or the dry mature seed of Fructus Pruni in fact), remove sarcocarp and core
Shell, takes out seed, is dried to obtain dry Semen Armeniacae Amarum under the conditions of temperature 35~45 DEG C;Dry Semen Armeniacae Amarum raw material employing ethanol is carried
Follow the example of and obtain extracting solution, more spray-dried method obtains Semen Armeniacae Amarum extract;The ethanol volume basis that described alcohol steep uses
Specific concentration is 70~80%, and raw material is 1:10~12 with the mass ratio of ethanol, and extraction temperature 80~85 DEG C, extraction time is 1
Hour.In gained Semen Armeniacae Amarum extract, in terms of amygdaloside, weight/mass percentage composition >=50.0%.
3, Radix Mori extract is to prepare according to the following steps: by fresh Radix Mori under the conditions of temperature 35~45 DEG C dried
Obtain Radix Mori and be dried raw material;Radix Mori is dried raw material uses ethanol extraction method to obtain Radix Mori extracting solution, more spray-dried method obtains
To Radix Mori extract;In described ethanol extraction method, Radix Mori is dried the mass ratio of raw material and ethanol is 1:10~12, the body of ethanol
Long-pending percent concentration is 85~95%.In gained Radix Mori extract, with 1-DNJ (1-Deoxynojirimycin,
DNJ) meter, weight/mass percentage composition >=0.5%.
4, salicylic acid extract is to prepare according to the following steps: torn by the raw material such as bark of willow etc. that salicylic acid content is abundant
It is immersed in water and is heated to boiling after becoming fritter, maintain about 20~30 minutes, filter;Filtrate heating is concentrated at the bottom of solution
Heating is stopped when portion has white crystal to produce.Then lower the temperature to solution with frozen water, it can be seen that more crystal separates out, and filters fall
The crystal that solution after temperature obtains is salicylic acid extract.Gained extract of willow bark in terms of salicylic acid, weight/mass percentage composition
>=50%.
5, plum extract is to prepare according to the following steps: fresh Armeniaca mume Sieb. employing ethanol extraction method is obtained Armeniaca mume Sieb. and extracts
Liquid, more spray-dried method obtains plum extract;In described ethanol extraction method, Armeniaca mume Sieb. raw material is 1 with the mass ratio of ethanol:
10~12, the concentration of volume percent of ethanol is 65~95%.In gained plum extract, with citrometer, percent mass contains
Amount >=2.5%.
6, Fructus Myricae rubrae extract is to prepare according to the following steps: fresh Waxberry fruit, bark or leaf are used ethanol extraction
Method obtains Fructus Myricae rubrae extracting solution, more spray-dried method obtains Fructus Myricae rubrae extract;Fructus Myricae rubrae raw material and ethanol in described ethanol extraction method
Mass ratio be 1:10~12, the concentration of volume percent of ethanol is 65~95%.In gained Fructus Myricae rubrae extract, with ampelopsin
And citrometer, weight/mass percentage composition >=15% of ampelopsin, weight/mass percentage composition >=2.5% of citric acid.
7, paclobutrazol: produced by Hubei Di Xin chemical industry Manufacturing Co., Ltd, purity is more than 15%.
8, abscisic acid (ABA): produced by Chendu Inst. of Biology, Chinese Academy of Sciences, purity is more than 85%.
Embodiment 1:
Weigh respectively mass percent be 17.50% Coumarins extract, 15.50% Semen Armeniacae Amarum extract, 10.50%
Radix Mori extract, 15.50% salicylic acid extract, 15.25% plum extract, 20.75% Fructus Myricae rubrae extract, 3.25% multiple-effect
Azoles, 8 kinds of raw materials of 1.75% abscisic acid (ABA) are placed in blend tank, stir, and compositing aluminium bag i.e. obtains mixing after packing or being canned
Type Rice Panicle sprouts inhibitor.
Embodiment 2:
Weigh respectively mass percent be 17.50% Coumarins extract, 15.50% Semen Armeniacae Amarum extract, 10.50%
Radix Mori extract, 15.00% salicylic acid extract, 14.75% plum extract, 22.00% Fructus Myricae rubrae extract, 3.25% multiple-effect
Azoles, 8 kinds of raw materials of 1.5% abscisic acid (ABA) are placed in blend tank, stir, and compositing aluminium bag i.e. obtains mixing after packing or being canned
Type Rice Panicle sprouts inhibitor.
Embodiment 3:
Weigh respectively mass percent be 17.00% Coumarins extract, 16.00% Semen Armeniacae Amarum extract, 11.00%
Radix Mori extract, 15.00% salicylic acid extract, 14.50% plum extract, 22.00% Fructus Myricae rubrae extract, 3.00% multiple-effect
Azoles, 8 kinds of raw materials of 1.50% abscisic acid (ABA) are placed in blend tank, stir, and compositing aluminium bag i.e. obtains mixing after packing or being canned
Type Rice Panicle sprouts inhibitor.
Embodiment 4: sprout at inhibitor through composite water spike of rice of the present invention under " 368 " serial hybridization Oryza sativa L. natural growthing condition
After reason, fringe sprouts the situation of change of rate
The composite water spike of rice weighing embodiment 1 preparation sprouts inhibitor 1.00kg, by the heating of 200kg water, stirring and dissolving, uses
I.e. obtain the fringe that mass percent is 0.5% after Plastic Drum fill to sprout inhibitor and directly use liquid.Directly " winning excellent with liquid by above-mentioned
368 ", sky excellent 368 " and " Qiu You 368 " hybrid rice produce land for growing field crops (under natural growthing condition, Oryza sativa L. sprays the stage of yellow ripeness) spray 1
Secondary, spray usage amount for directly with liquid 50kg/ mu.The fringe investigating each serial hybridization Rice Production land for growing field crops after Oryza glutinosa maturation respectively is sprouted
Incidence rate.As it can be seen from table 1 spray the fringe of Oryza glutinosa after 1 fringe sprouts inhibitor the stage of yellow ripeness, to sprout inhibition obvious.Above-mentioned hybridization
Rice Combination title is provided by Golden Rice Seeds Co., Ltd. of Guangdong, and fringe sprouts the computing formula of incidence rate: fringe sprouts incidence rate %=
Bud paddy number/total Number of kernels × 100%.
Table 1 " 368 " serial hybridization Oryza sativa L. sprouts fringe after inhibitor processes through fringe and sprouts the situation of change of rate
Embodiment 5: " 998 " serial hybridization Oryza sativa L. sprouts inhibitor through composite water spike of rice of the present invention in artificial intelligence greenhouse
After process, fringe sprouts the situation of change of rate
The composite water spike of rice weighing embodiment 2 preparation sprouts inhibitor 1.00kg, by the heating of 200kg water, stirring and dissolving, uses
I.e. obtain the fringe that mass percent is 0.5% after Plastic Drum fill to sprout inhibitor and directly use liquid.Directly use liquid in the stage of yellow ripeness by above-mentioned
Spray at " Tianyou998 ", Qiu You 998 with dough stage " and " II excellent 998 " hybrid rice on (hybrid rice plant artificial intelligence
In greenhouse, regulate temperature, humidity simultaneously, make fringe sprout incidence rate and improve.) each 1 time.Spray usage amount for directly using liquid every time
50kg/ mu.The fringe investigating each serial hybridization Oryza sativa L. after Oryza glutinosa maturation respectively sprouts incidence rate.From table 2 it can be seen that the stage of yellow ripeness and wax
The ripe phase respectively sprays the fringe of Oryza glutinosa after 1 fringe sprouts inhibitor and sprouts inhibition clearly.Above-mentioned Hybrid Rice Combinations title is by extensively
East saves gold seed rice industry company limited and provides, and fringe sprouts the computing formula of incidence rate: fringe sprout incidence rate %=bud paddy number/total Number of kernels ×
100%.
Table 2 " 368 " serial hybridization Oryza sativa L. sprouts fringe after inhibitor processes through fringe and sprouts the situation of change of rate
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-described embodiment
Limit, the change made under other any spirit without departing from the present invention and principle, modify, substitute, combine, simplify,
All should be the substitute mode of equivalence, within being included in protection scope of the present invention.
Claims (10)
1. a composite water spike of rice sprouts inhibitor, it is characterised in that: this Rice Panicle sprout inhibitor by following by mass percentage
The raw material of meter is made:
A kind of composite water spike of rice the most according to claim 1 sprouts inhibitor, it is characterised in that: this composite water spike of rice is sprouted
Inhibitor is preferably made up of following raw material by mass percentage:
A kind of composite water spike of rice the most according to claim 1 and 2 sprouts inhibitor, it is characterised in that:
In described Coumarins extract, in terms of coumarin, weight/mass percentage composition >=10%;
In described Semen Armeniacae Amarum extract, in terms of amygdaloside, weight/mass percentage composition >=50.0%;
In described Radix Mori extract, in terms of 1-DNJ, weight/mass percentage composition >=0.5%;
In described salicylic acid extract, in terms of salicylic acid, weight/mass percentage composition >=50%;
In described plum extract, with citrometer, weight/mass percentage composition >=2.5%;
In described Fructus Myricae rubrae extract, with ampelopsin and citrometer, weight/mass percentage composition >=15% of ampelopsin, the matter of citric acid
Amount percentage composition >=2.5%;
The purity of described paclobutrazol is more than 15%;
The purity of described abscisic acid is more than 85%.
A kind of composite water spike of rice the most according to claim 1 and 2 sprouts inhibitor, it is characterised in that:
Described Coumarins extract is to prepare according to the following steps: by the raw material matter of coumarin kind compound rich content
Amount percentage concentration 0.5% sodium hydrate aqueous solution heating extraction, removes impurity with ether after extracting solution cooling again, and then acid adding is adjusted
Joint pH, to neutral, is acidified after concentration again, and Coumarins and glycosides thereof can separate out, and is dried and separates out crystallization Coumarins extract;Institute
The raw material stating coumarin kind compound rich content is Melilotus suaveolens Ledeb., Radix Peucedani, Herba Cichorii, the Radix Angelicae Dahuricae, Fructus Psoraleae, tonka beans or Cymbidium ensifolium (L.) Sw.;Institute
The mass ratio stating raw material and sodium hydrate aqueous solution is 1:10~12.
A kind of composite water spike of rice the most according to claim 1 and 2 sprouts inhibitor, it is characterised in that:
Described Semen Armeniacae Amarum extract is to prepare according to the following steps: use the ripe Semen Armeniacae Amarum fruit gathered summer, removes fruit
Meat and nucleocapsid, take out seed, be dried to obtain dry Semen Armeniacae Amarum raw material under the conditions of temperature 35~45 DEG C;By dry Semen Armeniacae Amarum raw material
Use ethanol extraction method to obtain extracting solution, more spray-dried method obtains Semen Armeniacae Amarum extract;Described ethanol extraction method is dried
Semen Armeniacae Amarum raw material is 1:10~12 with the mass ratio of ethanol, and the concentration of volume percent of ethanol is 65~95%;Described bitter Fructus Pruni
A kind of fruit, such as apple, pear, etc. is to use rosaceous plant Prunus armeniaca L.var.ansu Maxim., Siberia Fructus Pruni, prunus mandshuricaKoehne or the dry mature seed of Fructus Pruni in fact.
A kind of composite water spike of rice the most according to claim 1 and 2 sprouts inhibitor, it is characterised in that:
Described Radix Mori extract is to prepare according to the following steps: obtain dried under the conditions of temperature 35~45 DEG C for fresh Radix Mori
It is dried raw material to Radix Mori;Radix Mori is dried raw material uses ethanol extraction method to obtain Radix Mori extracting solution, more spray-dried method obtains
Radix Mori extract;In described ethanol extraction method, Radix Mori is dried the mass ratio of raw material and ethanol is 1:10~12, the volume of ethanol
Percent concentration is 65~95%;Described fresh Radix Mori is the fresh Radix Mori using Guangdong mulberry product in winter.
A kind of composite water spike of rice the most according to claim 1 and 2 sprouts inhibitor, it is characterised in that:
Described salicylic acid extract is to prepare according to the following steps: soak after the raw material that salicylic acid content is abundant is torn into fritter
Duck in drink and be heated to boiling, maintaining 20~30 minutes, filtering;Filtrate heating being concentrated into bottom solution has white crystal to produce
Heating is stopped time raw;Then lowering the temperature to solution with frozen water, separate out crystal, the crystal being filtrated to get is salicylic acid extract;Institute
The raw material stating salicylic acid content abundant is willow, willow, Ilicis Purpureae bark or kiwifruit fruit.
A kind of composite water spike of rice the most according to claim 1 and 2 sprouts inhibitor, it is characterised in that:
Described plum extract is to prepare according to the following steps: fresh Armeniaca mume Sieb. employing ethanol extraction method is obtained Armeniaca mume Sieb. and extracts
Liquid, more spray-dried method obtains plum extract;In described ethanol extraction method, Armeniaca mume Sieb. raw material is 1 with the mass ratio of ethanol:
10~12, the concentration of volume percent of ethanol is 65~95%;Described Armeniaca mume Sieb. is the Armeniaca mume Sieb. that is produced from Guangdong;
Described Fructus Myricae rubrae extract is to prepare according to the following steps: the fruit of new arbutus, bark or leaf are used ethanol extraction
Method obtains Fructus Myricae rubrae extracting solution, more spray-dried method obtains Fructus Myricae rubrae extract;Fructus Myricae rubrae raw material and ethanol in described ethanol extraction method
Mass ratio be 1:10~12, the concentration of volume percent of ethanol is 65~95%;Described Fructus Myricae rubrae is the Fructus Myricae rubrae of Zhejiang cultivation
Fruit tree.
9. sprout the preparation method of inhibitor according to the composite water spike of rice described in any one of claim 1~8, it is characterised in that bag
Include following operating procedure: weigh all raw materials by said ratio and be placed in blend tank, stir, compositing aluminium bag packaging or canned
After i.e. obtain composite water spike of rice and sprout inhibitor.
10. sprout inhibitor answering in suppression Rice Panicle is sprouted according to the composite water spike of rice described in any one of claim 1~8
With.
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