CN108739847A - Bud inhibition agent and preparation method thereof on a kind of hybrid rice fringe - Google Patents
Bud inhibition agent and preparation method thereof on a kind of hybrid rice fringe Download PDFInfo
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- CN108739847A CN108739847A CN201810587574.1A CN201810587574A CN108739847A CN 108739847 A CN108739847 A CN 108739847A CN 201810587574 A CN201810587574 A CN 201810587574A CN 108739847 A CN108739847 A CN 108739847A
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- hybrid rice
- inhibition agent
- fringe
- bud inhibition
- bud
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The present invention provides bud inhibition agent on a kind of hybrid rice fringe, raw material includes vitamin, plant extracts, Osmolyte regulator, growth inhibitor, surfactant and water, vitamin is phytic acid, plant extracts is cumarin, Osmolyte regulator is NaCl, growth inhibitor is abscisic acid, and surfactant is polysorbas20.The method for preparing bud inhibition agent on hybrid rice fringe is to weigh each raw material according to the ratio, sequentially adds in water, pH is adjusted after stirring and evenly mixing.Bud inhibition agent prescription is at low cost on hybrid rice fringe provided by the present invention, and preparation method is simple, nontoxic, long-time service will not destroy ecological environment, its follow-up sprouting quality is not influenced, it is easy to use effective, it is sprayed once in mist with sprayer in Grain Filling, soak time is short, dosage is minimum, and each raw material is water-soluble substances in formula, and liquid is odorless, longer term invariance can be stored, be suitable for the vast breeding of hybrid rice in China and producing region uses.
Description
Technical field
The invention belongs to rice paddy seed production fields, and in particular to bud inhibition agent and its preparation side on a kind of hybrid rice fringe
Method.
Background technology
Rice seed germination is period the most key in seed plant ontogeny, directly affects the plantation of rice and miscellaneous
Hand over the production of hybrid seeds.A kind of mode that bud is sprouted as seed on Rice Panicle refers to that some cereal crops are met day after day in the grouting middle and later periods
Wet weather causes seed on fringe the phenomenon that rudiment (Pre-harvest sprouting, abbreviation PHS) before harvest.China hybridizes
Rice seed farm majority, since its rest period is generally shorter or unobvious, is harvesting behind south, the Grain Ripening of hybrid rice
It is preceding to meet with rainy for days and high temperature, high humidity weather often, in addition 920 are sprayed in the jointing stage to improve the outcrossing rate of sterile line
(GA3) aftereffect caused by, easily leads to head sprouting.The cardinal symptom of bud shows as head sprouting and sees blueness on fringe, does not germinate
But the seed viability that inside is sprouted also declines, and seriously affects rice quality and seed quality, and then generate to subsequent use
It influences.Bud rate is 5% or so on normal year fringe, and special time (high temperature and rainy) can be more than 20%, and a large amount of due to " 920 " make
With in addition the shallow feature with seed grain husk easy to crack of dormant trait specific to sterile line, fringe are sprouted rate up to 60%, be may be up to when serious
95% or more.According to investigations, bud makes breeding of hybrid rice yield reduce 1-2 at serious to be more than half, and every year on average on fringe
Hybrid rice seed makes the amplitude that viability declines lead to germination percentage and seed percentage establishment of seedling 10% or more because internal physiological changes
It reduces, the gently then underproduction is heavy then influence hybrid rice cultivated area and can not complete grain-production plan.Therefore, how effectively anti-
Only bud has become breeding of hybrid rice and further increases hybrid rice yield urgent problem on fringe.
It is dedicated to the research that cereal crop fringe is sprouted there are many seed biologist and crop breeding scholar both at home and abroad, and from
Twentieth century starts just to have held international cereal Sui Shang buds organizing committee the seventies, researchers from rice paddy seed water content,
Environmental condition (temperature, moisture, mineral nutrition etc.), hydrolytic enzyme activities, plant hormone, hereditary capacity and major gene resistance and minor effect base
Regulation and control of cause etc. on Rice Panicle the phenomenon that bud to conducting extensive research, to explain bud phenomenon on Rice Panicle and finding on fringe
Bud inhibition agent provides reference.CN106172523A discloses a kind of Green Water spike of rice and sprouts inhibitor and preparation method thereof, the rice
Fringe is sprouted inhibitor and is made of red bayberry extract, Semen Armeniacae Amarum extract, mulberry root extract, salicylic acid extract and plum extract,
The characteristic that raw material is each provided with rationally is utilized in the formula, and rationally assembling, which makes it play comprehensive fringe, sprouts inhibition comprehensive function, through reality
Verification sprouts inhibition clearly to Rice Panicle, although the inhibitor of invention offer is safe and can prevent fringe from sprouting,
Each extract is more troublesome acquisition process.CN106106548A discloses a kind of composite water spike of rice and sprouts inhibitor and its preparation
Methods and applications, the Rice Panicle sprout inhibitor by Coumarins extraction, Semen Armeniacae Amarum extract, the extraction of mulberry root, salicylic acid extract,
Plum extract, red bayberry extract, paclobutrazol and abscisic acid composition, the inhibitor which provides can prevent fringe from sprouting, however need
The extract wanted is still more, and extract preparation process is more troublesome.
To sum up, still need to develop bud inhibition agent on a kind of new hybrid rice fringe, the formula of the inhibitor answers of low cost, side
Just effectively, nontoxic and do not influence its and follow-up sprout quality.
Invention content
To solve problems of the prior art, an object of the present invention is to provide bud suppression on a kind of hybrid rice fringe
Preparation.
The second object of the present invention is to provide the preparation method of above-mentioned inhibitor.
To achieve the above object, the present invention uses following technical scheme：
Bud inhibition agent on a kind of hybrid rice fringe, includes the raw material of following quality permillage meter：
Vitamin 2~25 ‰,
Plant extracts 0.01~1 ‰,
Osmolyte regulator 0.2~3 ‰,
Surfactant 0.1~0.6 ‰,
Surplus is water.
Preferably, on the fringe bud inhibition agent include following quality permillage meter raw material：
Vitamin 5~25 ‰,
Plant extracts 0.1~1 ‰,
Osmolyte regulator 0.2~0.5 ‰,
Surfactant 0.2~0.4 ‰,
Surplus is water.
Preferably, on the fringe bud inhibition agent further included based on quality permillage 0.001~0.2 ‰ growth inhibition
Agent.
It is further preferred that the growth inhibitor is abscisic acid.
Preferably, the vitamin is phytic acid.
Preferably, the plant extracts is cumarin.
Preferably, the Osmolyte regulator is NaCl.
Preferably, the surfactant is polysorbas20.
A method of bud inhibition agent on above-mentioned hybrid rice fringe being prepared, specially：Each raw material is weighed according to the ratio, is added successively
Enter mixing in water, adjusts pH.
Preferably, ranging from the 5.8~6.0 of the pH.
It is further preferred that it is HCl to adjust the reagent used in pH.
Abscisic acid (Abscisic acid, ABA) plays an important role to bud on fringe, be in plant most important hormone it
One, it can be with regulating growth of plants, while improving plant drought, salt tolerant etc. to the respond of adverse circumstance, while being also one
It is to cause bud dormancy, leaf abscission and inhibit growth etc. that kind, which inhibits growing plants hormone, main function, in seed dormancy, is sprouted
Important regulating and controlling effect is played in the abiotic stress response such as hair, stomata closing, arid, low temperature.
Phytic acid is one kind of vitamin B, and scientific name is-six phosphate of inositol also known as inositol hexaphosphate, is nature
The more important natural materials of middle generally existing are widely present in beans, cereal, the dry plants such as fruits and vegetables and fruit, especially
Its content highest in seed is mainly stored in the form of phosphate and inositol, is hardly stored in a free form, usually with two
Valence or Tricationic such as Ca2+、Mg2+And Mn2+Plasma is initially formed salt, then forms the blister with single layer with protein again
Bead, these blister beads are further collected as higher volume of orbicule, these orbicules are that phytic acid is main in organism
Deposition pattern.Phytic acid is soluble in second alcohol and water, is insoluble in anhydrous ether, chloroform and benzene.Phytic acid is faint yellow or brown syrup
Like liquid.Aqueous solution is highly acid.With adjusting pH value and cushioning effect.Phytic acid is heated to be decomposed, in 120 DEG C or less short time
Interior be heated is stable.Phytic acid is also very stable to light, but unstable to microorganism, and phytase can be broken down into inositol and phosphorus
Acid, it is very sensitive to yeast, easily destroyed by its fermentation.All there is phytic acid sequestering power, chelating to make in very wide pH value range
Power, related with the metal ion type of chelating, usually its chelation is stronger under acid and neutrallty condition.
Phytic acid has important role in the growth and development process of plant, in plant can with Mg, Ca, Mn, Fe,
Ba and Zn plasmas combine and form phytate, are gradually accumulated in the development of seed and maturation, energy is provided for plant growth
Amount promotes seed to sprout.When seed is sprouted, internal phytase dissociates phytate, in conjunction with metal ion be released, with
Meet the level of seed Phos in the needs and seed of every metabolic activity in germination process.Phytic acid has also assisted in cell
The processes such as interior ATP synthesis, signal transduction, DNA damage reparation and rna transport.Phytic acid can also rise in the transportational process of substance
Instrumentality.The effect of signal transduction and thin in animal and plant cells of the incomplete hydrolysate such as inositoltriphosphoric acid of phytic acid
Intracellular can be used as secondary courier's transmission.
Phytic acid and cumarin can effectively inhibit the sprouting of seed on this basis suitably to match phytic acid, cumarin and ABA
Than that can extend seed rest period or delay bud on seed ear.
Phytic acid used in instant component be by the inhibition to the endogenous alpha-amylase activity of hybrid rice seed to
Inhibit bud on fringe, meanwhile, phytic acid can also reduce free phosphoric acid in hybrid rice seed, inhibit the activity of hydrolase, weaken and form sediment
The reaction of powder enzymically decomposed starch improves the activity of amylosynthease, is conducive to the synthesis of starch.
Cumarin can prevent the hydrolysis of starch, and the matter and energy needed for the rice seed germination initial period is needed from endosperm
It is obtained in the decomposition of middle storage nutriment, wherein major storage substance is in starch.
Abscisic acid can improve enzymatic protective system-superoxide dismutase and hydrogen peroxide in plant in instant component
Enzymatic activity, while abscisic acid can act synergistically with phytic acid to inhibit the activity of seed endogenous hydrolytic enzymes.
NaCl, which can be used as Osmolyte regulator, used in instant component quickly reduces seed moisture content.
Polysorbas20, which is added, can make reagent be not easy to fall off from fringe, to promote inhibition.
Beneficial effects of the present invention
1, bud inhibition agent prescription is at low cost on hybrid rice fringe provided by the present invention, and preparation method is simple, nontoxic,
Long-time service will not destroy ecological environment, not influence its follow-up sprouting quality；
2, bud inhibition agent is easy to use effectively on hybrid rice fringe provided by the present invention, in Grain Filling sprayer
It is sprayed once in mist；
3, inhibitor dosage provided by the present invention is minimum, after area converts 20 liters of water dilutions with 100mL finished product liquids per acre
It can be used, each raw material is water-soluble substances in formula, and liquid is odorless, the longer term can store invariance, is suitable for China
Vast breeding of hybrid rice and producing region use.
Specific implementation mode
The following is specific embodiments of the present invention, and is further retouched to technical scheme of the present invention work in conjunction with the embodiments
It states, however, the present invention is not limited to these examples.
Embodiment 1
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Phytic acid 5g, cumarin 0.1g, abscisic acid 0.001g, NaCl 0.5g, polysorbas20 0.3g, water 994.099g.
Embodiment 2
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Phytic acid 25g, coumarin 1 g, abscisic acid 0.02g, NaCl 3g, polysorbas20 0.3g, water 970.68g.
Embodiment 3
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Phytic acid 20g, cumarin 0.2g, abscisic acid 0.003g, NaCl 0.5g, polysorbas20 0.3g, water 978.997g.
Comparative example 1
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Phytic acid 41.5g, water 958.5g.
Comparative example 2
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Cumarin 0.8g, water 999.2g.
Comparative example 3
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Abscisic acid 0.013g, water 999.987g.
Comparative example 4
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Phytic acid 20g, cumarin 0.2g, abscisic acid 0.003g, polysorbas20 0.3g, water 979.497g.
Comparative example 5
Bud inhibition agent on a kind of hybrid rice fringe, raw material and proportioning in every liter of inhibitor are：
Phytic acid 20g, cumarin 0.2g, abscisic acid 0.003g, water 979.797g.
Test example
By bud inhibition agent on the hybrid rice fringe in Examples 1 to 3 and comparative example 1~5, with control group (not spraying) into
Row compares, and is sprayed once to experiment rice in misty with sprayer in Grain Filling, 3 liters are converted with 15mL finished product liquids per square meter
It is used after water dilution.Fringe sprouts the variation of rate after comparing inhibited dose of processing, as a result such as table 1：
1 fringe of table sprouts rate comparison
| Number | Inhibitor is numbered | The total grain number of paddy | Fringe sprouts incidence |
| 1 | Embodiment 1 | 1000 | 52% |
| 2 | Embodiment 2 | 1000 | 34% |
| 3 | Embodiment 3 | 1000 | 46% |
| 4 | Comparative example 1 | 1000 | 72% |
| 5 | Comparative example 2 | 1000 | 61% |
| 6 | Comparative example 3 | 1000 | 62% |
| 7 | Comparative example 4 | 1000 | 50% |
| 8 | Comparative example 5 | 1000 | 54% |
| 9 | It compares (not spraying) | 1000 | 98% |
Using formula provided by the present invention and ratio, the synergistic effect between each raw material makes the inhibitor effect being prepared
It is optimal, the missing of raw material components or the excessive effect that can all influence inhibitor.Each reagent is excessively added, although initial stage inhibiting effect
Obviously, however harmful effect can be generated to later stage seed.
Claims (10)
1. bud inhibition agent on a kind of hybrid rice fringe, which is characterized in that include the raw material of following quality permillage meter：
Vitamin 2~25 ‰,
Plant extracts 0.01~1 ‰,
Osmolyte regulator 0.2~3 ‰,
Surfactant 0.1~0.6 ‰,
Surplus is water.
2. bud inhibition agent on hybrid rice fringe according to claim 1, which is characterized in that on the fringe bud inhibition agent include
The raw material of following quality permillage meter：
Vitamin 5~25 ‰,
Plant extracts 0.1~1 ‰,
Osmolyte regulator 0.2~0.5 ‰,
Surfactant 0.2~0.4 ‰,
Surplus is water.
3. bud inhibition agent on hybrid rice fringe according to claim 1 or 2, which is characterized in that bud inhibition agent on the fringe
0.001~0.2 ‰ growth inhibitor is further included based on quality permillage.
4. bud inhibition agent on hybrid rice fringe according to claim 3, which is characterized in that the growth inhibitor is to fall off
Acid.
5. bud inhibition agent on hybrid rice fringe according to claim 1 or 2, which is characterized in that the vitamin is phytic acid.
6. bud inhibition agent on hybrid rice fringe according to claim 1 or 2, which is characterized in that the plant extracts is
Cumarin.
7. bud inhibition agent on hybrid rice fringe according to claim 1 or 2, which is characterized in that the Osmolyte regulator is
NaCl。
8. bud inhibition agent on hybrid rice fringe according to claim 1 or 2, which is characterized in that the surfactant is
Polysorbas20.
9. a kind of method preparing the bud inhibition agent on hybrid rice fringe as described in any one of claim 1~8, which is characterized in that
The method is：Each raw material is weighed according to the ratio, sequentially adds mixing in water, adjusts pH.
10. the method for preparing bud inhibition agent on hybrid rice fringe according to claim 9, which is characterized in that the model of the pH
Enclose is 5.8~6.0.
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| CN201810587574.1A CN108739847B (en) | 2018-06-08 | 2018-06-08 | Hybrid rice ear bud-growing inhibitor and preparation method thereof |
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| CN201810587574.1A CN108739847B (en) | 2018-06-08 | 2018-06-08 | Hybrid rice ear bud-growing inhibitor and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111955459A (en) * | 2020-08-20 | 2020-11-20 | 浙江大学 | Hybrid rice pre-harvest sprouting inhibitor, preparation method thereof and method for inhibiting hybrid rice pre-harvest sprouting |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773137A (en) * | 2010-01-26 | 2010-07-14 | 湖南农业大学 | Hybrid rice pre-harvest sprouting inhibitor |
| CN106106548A (en) * | 2016-06-28 | 2016-11-16 | 广东省农业科学院水稻研究所 | A kind of composite water spike of rice sprouts inhibitor and its preparation method and application |
-
2018
- 2018-06-08 CN CN201810587574.1A patent/CN108739847B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101773137A (en) * | 2010-01-26 | 2010-07-14 | 湖南农业大学 | Hybrid rice pre-harvest sprouting inhibitor |
| CN106106548A (en) * | 2016-06-28 | 2016-11-16 | 广东省农业科学院水稻研究所 | A kind of composite water spike of rice sprouts inhibitor and its preparation method and application |
Non-Patent Citations (1)
| Title |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111955459A (en) * | 2020-08-20 | 2020-11-20 | 浙江大学 | Hybrid rice pre-harvest sprouting inhibitor, preparation method thereof and method for inhibiting hybrid rice pre-harvest sprouting |
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