CN113528632A - 用于多核苷酸测序的组合物和方法 - Google Patents

用于多核苷酸测序的组合物和方法 Download PDF

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Publication number
CN113528632A
CN113528632A CN202110859489.8A CN202110859489A CN113528632A CN 113528632 A CN113528632 A CN 113528632A CN 202110859489 A CN202110859489 A CN 202110859489A CN 113528632 A CN113528632 A CN 113528632A
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CN
China
Prior art keywords
polynucleotide
sequence
translocation
helicase
pore
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CN202110859489.8A
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English (en)
Chinese (zh)
Inventor
埃里克·斯塔瓦
简斯·H·冈拉克
杰弗里·G·曼德尔
凯文·L·冈德森
伊恩·M·德灵顿
侯赛因·莫西玛尼
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Illumina Inc
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Illumina Inc
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Publication of CN113528632A publication Critical patent/CN113528632A/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202110859489.8A 2013-11-26 2014-11-26 用于多核苷酸测序的组合物和方法 Pending CN113528632A (zh)

Applications Claiming Priority (3)

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US201361909316P 2013-11-26 2013-11-26
US61/909,316 2013-11-26
CN201480064725.5A CN105934522B (zh) 2013-11-26 2014-11-26 用于多核苷酸测序的组合物和方法

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CN113528632A true CN113528632A (zh) 2021-10-22

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CN201480064725.5A Active CN105934522B (zh) 2013-11-26 2014-11-26 用于多核苷酸测序的组合物和方法

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US (5) US9689033B2 (https=)
EP (3) EP3556869B1 (https=)
JP (4) JP6800749B2 (https=)
CN (2) CN113528632A (https=)
AU (3) AU2014354726B2 (https=)
CA (2) CA2926871C (https=)
DK (2) DK3074534T3 (https=)
ES (2) ES2958715T3 (https=)
FI (1) FI3556869T3 (https=)
LT (1) LT3074534T (https=)
SI (1) SI3074534T1 (https=)
WO (2) WO2015081178A1 (https=)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
BR112014020211A2 (pt) 2012-02-16 2017-07-04 Oxford Nanopore Tech Ltd métodos para analisar uma série ordenada por tempo de medições de um polímero, para estimar a presença, ausência ou quantidade de um polímero alvo, e para determinar uma alteração em um polímero, programa de computador, e, dispositivos de análise e de diagnóstico
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US10213746B2 (en) 2016-04-14 2019-02-26 Lockheed Martin Corporation Selective interfacial mitigation of graphene defects
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
EP3556869B1 (en) * 2013-11-26 2023-08-02 Illumina Inc. Compositions and methods for polynucleotide sequencing
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
ES3035698T3 (en) * 2014-02-19 2025-09-08 Univ Washington Nanopore-based analysis of protein characteristics
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
EP3971300B1 (en) * 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
EP3334840A1 (en) * 2015-08-10 2018-06-20 Stratos Genomics, Inc. Single molecule nucleic acid sequencing with molecular sensor complexes
JP2018533010A (ja) * 2015-10-21 2018-11-08 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft ナノポアベースのdna配列決定のための脂質二重層形成を支援するための撥水性層としてのフルオロポリマーの使用
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
EP3475447B1 (en) * 2016-06-23 2021-04-21 F. Hoffmann-La Roche AG Formation and calibration of nanopore sequencing cells
WO2018029108A1 (en) 2016-08-08 2018-02-15 F. Hoffmann-La Roche Ag Basecalling for stochastic sequencing processes
US20180127816A1 (en) 2016-10-19 2018-05-10 Illumina, Inc. Methods for chemical ligation of nucleic acids
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
WO2019002893A1 (en) 2017-06-30 2019-01-03 Vib Vzw NEW PROTEIN PORES
CA3067230A1 (en) * 2017-09-15 2019-03-21 Illumina, Inc. Tuning and calibration features of a sequence-detection system
JP2020530257A (ja) * 2017-09-15 2020-10-22 イルミナ インコーポレイテッド 配列検出システム
US10310959B2 (en) 2017-11-07 2019-06-04 Bank Of America Corporation Pre-deployment validation system using intelligent databases
JP7377222B2 (ja) * 2018-06-21 2023-11-09 エフ. ホフマン-ラ ロシュ アーゲー 配列決定のためのトンネル接合部
EP3814529A1 (en) * 2018-06-26 2021-05-05 Electronic Biosciences Inc. Controlled nanopore translocation utilizing extremophilic replication proteins
MY204234A (en) 2018-08-27 2024-08-16 Regeneron Pharma Use of raman spectroscopy in downstream purification
JP2020031557A (ja) * 2018-08-28 2020-03-05 株式会社日立ハイテクノロジーズ 生体分子分析装置
WO2020084705A1 (ja) * 2018-10-24 2020-04-30 株式会社日立ハイテク 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
WO2021056598A1 (zh) 2019-09-29 2021-04-01 北京齐碳科技有限公司 一种Mmup单体变体及其应用
EP4061965A1 (en) * 2019-11-19 2022-09-28 Rijksuniversiteit Groningen Artificial nanopores and uses and methods relating thereto
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN114807317A (zh) * 2021-01-22 2022-07-29 上海羿鸣生物科技有限公司 一种优化的dna线性扩增方法及试剂盒
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
CN113322180B (zh) * 2021-05-31 2022-07-15 中国科学院物理研究所 基于纳米孔测序的力谱分析方法和分析装置
WO2023164728A2 (en) * 2022-02-28 2023-08-31 Rajant Health Incorporated Systems and methods for alignment-free variant calling
IL320502A (en) 2022-10-28 2025-06-01 Univ Groningen Nanopore-based protein efflux
EP4698646A2 (en) * 2023-04-21 2026-02-25 ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY 1475 North Scottsdale Road Novel transmembrane sensors and method of characterization for lysis-free detection of intracellular targets
WO2026073207A1 (en) 2024-09-30 2026-04-02 Illumina, Inc. Affinity-binder-based assay compositions and methods
CN119639558B (zh) * 2024-12-13 2025-09-26 杭州海兰时生物科技有限责任公司 一种用于单细胞测序的液滴融合系统

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013057495A2 (en) * 2011-10-21 2013-04-25 Oxford Nanopore Technologies Limited Enzyme method

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
US5508179A (en) 1994-03-18 1996-04-16 Bio-Rad Laboratories, Inc. Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP2003502166A (ja) 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
EP1990428B1 (en) 2000-02-07 2010-12-22 Illumina, Inc. Nucleic acid detection methods using universal priming
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
WO2010004265A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CA3092369C (en) 2008-09-22 2025-09-23 University Of Washington MSP NANOPORES AND ASSOCIATED PROCESSES
GB0905140D0 (en) * 2009-03-25 2009-05-06 Isis Innovation Method
EP2614156B1 (en) * 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
EP3385992B1 (en) 2011-07-27 2024-09-04 The Board of Trustees of the University of Illinois Nanopore sensors for biomolecular characterization
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
JP5809572B2 (ja) 2012-01-30 2015-11-11 ルネサスエレクトロニクス株式会社 半導体装置
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US9116118B2 (en) * 2012-06-08 2015-08-25 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
EP2875128B8 (en) * 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
EP3556869B1 (en) * 2013-11-26 2023-08-02 Illumina Inc. Compositions and methods for polynucleotide sequencing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013057495A2 (en) * 2011-10-21 2013-04-25 Oxford Nanopore Technologies Limited Enzyme method

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FI3556869T3 (fi) 2023-09-28
JP2023116553A (ja) 2023-08-22
EP3556869B1 (en) 2023-08-02
AU2022231786B2 (en) 2025-04-17
US20200024657A1 (en) 2020-01-23
WO2015081211A3 (en) 2015-08-06
EP3556869A1 (en) 2019-10-23
AU2020202489B2 (en) 2022-06-23
JP2025072503A (ja) 2025-05-09
LT3074534T (lt) 2019-08-26
EP3074534B1 (en) 2019-05-01
ES2958715T3 (es) 2024-02-13
US11879155B2 (en) 2024-01-23
ES2735015T3 (es) 2019-12-13
CA3157586C (en) 2026-02-03
SI3074534T1 (sl) 2019-08-30
EP4282983A3 (en) 2024-02-28
DK3556869T3 (da) 2023-10-02
CN105934522B (zh) 2021-07-20
US20210355534A1 (en) 2021-11-18
WO2015081211A2 (en) 2015-06-04
JP2021040646A (ja) 2021-03-18
WO2015081178A1 (en) 2015-06-04
CN105934522A (zh) 2016-09-07
US11041196B2 (en) 2021-06-22
CA3157586A1 (en) 2015-06-04
AU2014354726B2 (en) 2020-03-12
AU2022231786A1 (en) 2022-10-13
US10364462B2 (en) 2019-07-30
US20240167085A1 (en) 2024-05-23
AU2020202489A1 (en) 2020-05-07
US20150152495A1 (en) 2015-06-04
US20170268055A1 (en) 2017-09-21
JP2017503473A (ja) 2017-02-02
AU2014354726A1 (en) 2016-06-02
CA2926871C (en) 2022-08-16
US9689033B2 (en) 2017-06-27
EP4282983A2 (en) 2023-11-29
JP7349972B2 (ja) 2023-09-25
EP3074534A1 (en) 2016-10-05
DK3074534T3 (da) 2019-07-29
CA2926871A1 (en) 2015-06-04
JP6800749B2 (ja) 2020-12-23

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Application publication date: 20211022