CN113519712A - Nutritional type anti-substitution feed additive for preventing African swine fever and preparation method thereof - Google Patents

Nutritional type anti-substitution feed additive for preventing African swine fever and preparation method thereof Download PDF

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CN113519712A
CN113519712A CN202110832176.3A CN202110832176A CN113519712A CN 113519712 A CN113519712 A CN 113519712A CN 202110832176 A CN202110832176 A CN 202110832176A CN 113519712 A CN113519712 A CN 113519712A
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parts
yeast
feed additive
swine fever
african swine
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张洋
马小林
何泰
王惠
谢妮
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Xi'an Institute Of Foruminant
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements

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  • Life Sciences & Earth Sciences (AREA)
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  • Polymers & Plastics (AREA)
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  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention discloses a nutritional type anti-substitution feed additive for preventing African swine fever, which comprises the following raw materials in parts by weight: 20-30 parts of inulin, 10-30 parts of protease, 20-30 parts of glucan, 20-30 parts of vitamin C, 0.05-2 parts of yeast iron, 0.05-2 parts of yeast selenium and 0.05-2 parts of yeast zinc; the preparation method comprises the following steps: (1) weighing raw materials; (2) adding yeast iron, yeast selenium and yeast zinc into dextran, mixing, adding protease into inulin, mixing, adding vitamin C, mixing, and sieving. The invention mixes the natural plant extract inulin, protease, glucan, vitamin C and organic trace elements of yeast iron, yeast selenium and yeast zinc, blocks the replication of virus in the animal body and activates the autoimmune system of the animal, provides trace elements necessary for biochemical reaction for the animal and has definite effect on preventing African swine fever.

Description

Nutritional type anti-substitution feed additive for preventing African swine fever and preparation method thereof
Technical Field
The invention relates to the technical field of animal feed, in particular to a nutritional type anti-substitution feed additive for preventing African swine fever and a preparation method thereof.
Background
African Swine Fever (ASF) is an acute, hemorrhagic and virulent infectious disease caused by African Swine Fever Virus (ASFV) infecting domestic pigs and various wild pigs (such as African wild pigs and European wild pigs). The world animal health Organization (OIE) classifies the animal epidemic disease as a legal report animal epidemic disease, and the disease is also a type of animal epidemic disease which is mainly prevented in China. The clinical symptoms of African swine fever are similar to those of swine fever, and diagnosis can be confirmed only by means of laboratory monitoring. 68 proved by China that epidemic sources have already found out the epidemic situation of the domestic pigs, and the transmission ways are mainly three: first, live pigs and their products are distributed and transported across regions, accounting for about 19% of all epidemic situations; secondly, feeding pigs with kitchen residues, wherein the kitchen residues account for about 34% of the whole epidemic situation; and thirdly, people and vehicles carry toxic spread, which is the most main mode for spreading the current epidemic situation and accounts for about 46 percent of the whole epidemic situation.
At present, the prevention of African swine fever is mainly based on enhancing the biological safety. For an African swine fever positive pig farm, the method of removing positive pigs by periodically detecting a tooth extraction mode is adopted for pig herd purification. For the drug prevention of African swine fever, the traditional Chinese medicines are mainly added, but the difference of the prevention effect is large, and no drug and feed additive specially used for preventing the African swine fever virus exists.
Therefore, how to provide a nutritional substitute feed additive for preventing African swine fever is a problem to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In view of the above, the present invention aims to provide a nutritional substitute antibiotic feed additive for preventing african swine fever and a preparation method thereof, wherein the additive is prepared by taking various natural plant extracts as raw materials and adding organic trace elements, so as to solve the defects in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a nutritional anti-substitution feed additive for preventing African swine fever comprises the following raw materials in parts by weight: 20-30 parts of inulin, 10-30 parts of protease, 20-30 parts of glucan, 20-30 parts of vitamin C, 0.05-2 parts of yeast iron, 0.05-2 parts of yeast selenium and 0.05-2 parts of yeast zinc;
preferably: 25 parts of inulin, 24.9 parts of protease, 24.9 parts of glucan, 24.9 parts of vitamin C, 0.1 part of yeast iron, 0.1 part of yeast selenium and 0.1 part of yeast zinc.
The action mechanism of the invention is as follows:
after natural plant extracts of inulin, protease, glucan, vitamin C and organic trace elements of yeast iron, yeast selenium and yeast zinc are proportioned, replication of viruses in an animal body is blocked, an animal autoimmune system is activated, trace elements necessary for biochemical reaction are provided for the animal, and the preparation method has a definite effect on prevention of African swine fever.
Wherein inulin is reserve polysaccharide in plant, mainly comes from plant, and can greatly increase Ca2+、Mg2+、Zn2+、Cu2 +、Fe2+Absorption of iso-minerals; inulin can enhance gastrointestinal peristalsis, improve gastrointestinal function, promote digestion and appetite, and improve immunity; short chain fatty acids produced by fermentation of inulin in the colon can lower the pH of the colon, inhibit the growth of saprophytic bacteria, reduce the production of toxic products, and reduce the irritation to the intestinal wall.
Proteases are a generic term for a class of enzymes that hydrolyze peptide chains of proteins, and are key enzymes in protein digestion and absorption. The protease is added into the animal feed, so that the digestion and absorption of the animal to protein can be improved, the anti-nutritional factors in the feed can be reduced, the daily gain can be improved, the feed conversion ratio can be reduced, the occurrence rate of nutritional diarrhea can be reduced, the feed utilization rate can be improved, and the animal production performance can be improved.
The glucan is a homotype polysaccharide which is composed of glucose as monosaccharide, and glucose units are connected by glycosidic bonds. The glucan is added into the animal feed and swells in water to form a high-viscosity solution, so that the viscosity of gastrointestinal chyme is increased, the release and diffusion of nutrient substances are hindered, the activity of digestive enzyme is reduced, and the digestion and absorption of the nutrient substances are reduced; meanwhile, the feed additive can break plant cell walls, reduce the viscosity of chyme, improve the activity of digestive enzyme, promote the digestion and absorption of nutrient substances, reduce the fermentation of harmful microorganisms in hindgut, stabilize the quality of the feed, improve the utilization rate of the feed and improve the production performance of animals.
The vitamin C can effectively slow down stress and reduce the morbidity of swinery to ensure the healthy growth of the swinery, and particularly in the heat stress period, the vitamin C is added into the feed, so that the capillary permeability of the organism can be reduced, the disease resistance of the organism can be enhanced, the fatty degeneration of liver cells can be reduced, and the death rate can be reduced.
The yeast iron, the yeast selenium and the yeast zinc have higher protein content and are easy to absorb, so that the production performance can be improved; effectively promote growth, improve daily gain, feed remuneration and reproductive performance; the cost of the feed is reduced; the safety is good, the composition does not contain harmful bacteria such as salmonella, and is nontoxic and harmless to people, livestock, fish and shrimp, and has no environmental pollution and cross pollution; the performance is stable, the performance is stable when the premix is added into powder, granules and premix, the fluidity is good, no electrostatic adsorption exists, the premix can resist gastric acid and high granulation temperature, and the viable bacteria rate is high.
The preparation method of the nutritional type anti-substitution feed additive for preventing African swine fever specifically comprises the following steps:
(1) weighing the raw materials according to the weight parts of the nutritional type anti-substitution feed additive;
(2) firstly adding yeast iron, yeast selenium and yeast zinc into glucan for primary mixing, then adding protease into inulin for primary mixing, then mixing the two mixtures for the second time, finally adding vitamin C for third mixing, and sieving to obtain the nutritional type replacement-resistant feed additive for preventing African swine fever.
Further, in the step (2), the temperature of the first mixing is 20-25 ℃, preferably 23 ℃; the stirring speed is 28-34r/min, preferably 32 r/min; the time is 3-5min, preferably 3 min. The temperature of the second mixing is 20-25 ℃, and the preferred temperature is 20 ℃; the stirring speed is 28-32r/min, preferably 32 r/min; the time is 2-5min, preferably 3 min. The temperature of the third mixing is 18-25 ℃, and the preferred temperature is 20 ℃; the stirring speed is 30-32r/min, preferably 32 r/min; the time is 2-4min, preferably 3 min. The mesh number of the sieved mesh is 80-100 meshes, and the preferred mesh is 80 meshes.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
the anti-feed additive can effectively reduce the death rate of pigs, improve the survival rate of lean and weak pigs and improve the economic benefit of a farm.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The nutritional anti-substitution feed additive for preventing African swine fever comprises the following raw materials by weight: 20kg of inulin, 20kg of protease, 30kg of glucan, 29.85kg of vitamin C, 0.05kg of yeast iron, 0.05kg of yeast selenium and 0.05kg of yeast zinc.
The preparation method of the nutritional type anti-substitution feed additive for preventing African swine fever specifically comprises the following steps:
(1) weighing the raw materials according to the weight of the nutritional type anti-substitution feed additive;
(2) firstly adding yeast iron, yeast selenium and yeast zinc into glucan, mixing for 3min at the temperature of 25 ℃ at the stirring speed of 30r/min, then adding protease into inulin, mixing for 3min at the temperature of 25 ℃ at the stirring speed of 30r/min, then mixing the two mixtures for 3min at the temperature of 22 ℃ at the stirring speed of 32r/min, finally adding vitamin C, mixing for 3min at the temperature of 20 ℃ at the stirring speed of 32r/min, and sieving with a 80-mesh sieve to obtain the nutritional substitute-resistant feed additive for preventing African swine fever.
Example 2
The nutritional anti-substitution feed additive for preventing African swine fever comprises the following raw materials by weight: 25kg of inulin, 24.9kg of protease, 24.9kg of glucan, 24.9kg of vitamin C, 0.1kg of yeast iron, 0.1kg of yeast selenium and 0.1kg of yeast zinc.
The preparation method of the nutritional type anti-substitution feed additive for preventing African swine fever specifically comprises the following steps:
(1) weighing the raw materials according to the weight of the nutritional type anti-substitution feed additive;
(2) firstly adding yeast iron, yeast selenium and yeast zinc into glucan, mixing for 3min at the temperature of 20 ℃ and the stirring speed of 32r/min, then adding protease into inulin, mixing for 3min at the temperature of 20 ℃ and the stirring speed of 32r/min, then mixing the two mixtures for 3min at the temperature of 25 ℃ and the stirring speed of 30r/min, finally adding vitamin C, mixing for 3min at the temperature of 20 ℃ and the stirring speed of 32r/min, and sieving with a 80-mesh sieve to obtain the nutritional substitute-resistant feed additive for preventing African swine fever.
Example 3
The nutritional anti-substitution feed additive for preventing African swine fever comprises the following raw materials by weight: 30kg of inulin, 10kg of protease, 30kg of glucan, 24kg of vitamin C, 2kg of yeast iron, 2kg of yeast selenium and 2kg of yeast zinc.
The preparation method of the nutritional type anti-substitution feed additive for preventing African swine fever specifically comprises the following steps:
(1) weighing the raw materials according to the weight of the nutritional type anti-substitution feed additive;
(2) firstly adding yeast iron, yeast selenium and yeast zinc into glucan, mixing for 3min at the temperature of 20 ℃ and the stirring speed of 30r/min, then adding protease into inulin, mixing for 3min at the temperature of 20 ℃ and the stirring speed of 30r/min, then mixing the two mixtures for 3min at the temperature of 25 ℃ and the stirring speed of 30r/min, finally adding vitamin C, mixing for 3min at the temperature of 20 ℃ and the stirring speed of 32r/min, and sieving with a 100-mesh sieve to obtain the nutritional substitute-resistant feed additive for preventing African swine fever.
Performance testing
Selecting a certain pig farm in the rural area of Xian, removing the columns on the two sides and only the positive pigs, dividing the remaining 9 columns into 2 groups, wherein the first group is 6 columns (241 heads), and using the nutritional type anti-substitution feed additive prepared in the embodiment 2; the second group is 3 columns (139 heads) using astragalus polysaccharides plus multivitamins.
Wherein, the first phase (10 days): the first group, using the nutritional type anti-feed additive prepared in example 2 30 g/head/day; in the second group, astragalus polysaccharide 5 g/head/day + multi-vitamin 5 g/head/day was used.
Second stage (10 days): the first group, using the nutritional anti-feed additive prepared in example 2 15 g/head/day; in the second group, astragalus polysaccharide 5 g/head/day + multi-vitamin 5 g/head/day was used.
1. During the test, the death (including the number of dead heads and the mortality) of pigs in each stage and each group needs to be recorded. The results are shown in Table 1.
TABLE 1 mortality of pigs of the first and second groups
Figure BDA0003175938620000061
As can be seen from table 1, the first stage mortality decreased 8.45%, the second stage mortality decreased 0.09%, and the total mortality decreased 7.84% compared to the second group.
The tests show that the nutritional anti-substitution feed additive prepared in the embodiment 2 can effectively reduce the death rate of negative pigs in an African swine fever pig farm.
2. In the test process, sampling and inspection are carried out on each group of columns every week, and the infection condition is evaluated: the oral liquid is collected by using gauze strips for sample collection, the African swine fever pathogen is detected, one sample is arranged in each column, and gloves are replaced in each column during sampling. The results are shown in Table 2.
TABLE 2 survival rate and test results of lean and weak pigs of the first and second groups
Recording items First group Second group
Survival rate of lean pig (%) 18.91 14.29
3.18 first test Negative of Negative of
3.25 second test Negative of Negative of
3.31 third test Negative of Negative of
As shown in Table 2, the survival rate of the first group of the lean and weak pigs was increased by 4.62% compared with the second group, and the three tests were negative.
The above experiments show that the nutritional anti-substitution feed additive prepared in example 2 of the invention has a better effect of improving the survival rate of lean and weak pigs than astragalus polysaccharide, and both of the additive and the additive can prevent African swine fever.
3. During the test, the feeding condition, hair color and mental state of each group of pigs were recorded. The results are shown in Table 3.
TABLE 3 ingestion, hair color and mental status of the first and second groups of pigs
Figure BDA0003175938620000071
As can be seen from Table 3, the health status of the pigs was significantly improved, showing a bright hair color and a good mental status, with the first group of effects being more significant than the second group.
The above experiments show that the nutritional anti-substitution feed additive and astragalus polysaccharide prepared in example 2 of the present invention can increase feed intake and promote health, and the former effect is more obvious.
In conclusion, the anti-feed additive can effectively reduce the death rate of the pigs, improve the survival rate of the lean and weak pigs and improve the economic benefit of the farm.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (7)

1. The nutritional type anti-substitution feed additive for preventing African swine fever is characterized by comprising the following raw materials in parts by weight: 20-30 parts of inulin, 10-30 parts of protease, 20-30 parts of glucan, 20-30 parts of vitamin C, 0.05-2 parts of yeast iron, 0.05-2 parts of yeast selenium and 0.05-2 parts of yeast zinc.
2. The nutritional replacement resistance feed additive for preventing African swine fever according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 25 parts of inulin, 24.9 parts of protease, 24.9 parts of glucan, 24.9 parts of vitamin C, 0.1 part of yeast iron, 0.1 part of yeast selenium and 0.1 part of yeast zinc.
3. The preparation method of the nutritional type anti-substitution feed additive for preventing African swine fever is characterized by comprising the following steps:
(1) weighing the raw materials according to the weight parts of the nutritional type anti-substitution feed additive of claim 1 or 2;
(2) adding yeast iron, yeast selenium and yeast zinc into glucan for first mixing, adding protease into inulin for first mixing, then mixing the two mixtures for the second time, adding vitamin C for third mixing, and sieving to obtain the nutritional type replacement-resistant feed additive for preventing African swine fever.
4. The method for preparing the nutritional substitute feed additive for preventing African swine fever according to claim 3, wherein in the step (2), the temperature of the first mixing is 20-25 ℃, the stirring speed is 28-34r/min, and the time is 3-5 min.
5. The method for preparing the nutritional substitute feed additive for preventing African swine fever according to claim 3, wherein in the step (2), the temperature of the second mixing is 20-25 ℃, the stirring speed is 28-32r/min, and the time is 2-5 min.
6. The method for preparing the nutritional substitute feed additive for preventing African swine fever according to claim 3, wherein in the step (2), the temperature of the third mixing is 18-25 ℃, the stirring speed is 30-32r/min, and the time is 2-4 min.
7. The method for preparing the nutritional substitute feed additive for preventing African swine fever according to claim 3, wherein in the step (2), the mesh number of the sieved screen is 80-100 meshes.
CN202110832176.3A 2021-07-22 2021-07-22 Nutritional type anti-substitution feed additive for preventing African swine fever and preparation method thereof Pending CN113519712A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101669575A (en) * 2008-09-10 2010-03-17 陈健雄 Feed additive for improving disease resistance of livestock and poultry and production method thereof
CN106306346A (en) * 2015-06-26 2017-01-11 上海杰隆生物工程股份有限公司 Feeding method of live pigs without using feed antibiotics
CN111772046A (en) * 2020-07-14 2020-10-16 江西大佑农生物科技有限公司 Reinforced big pig compound premix feed
CN111789192A (en) * 2020-06-28 2020-10-20 天津瑞益瑞美生物技术有限公司 Functional additive for improving reproductive performance of breeding pigs and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101669575A (en) * 2008-09-10 2010-03-17 陈健雄 Feed additive for improving disease resistance of livestock and poultry and production method thereof
CN106306346A (en) * 2015-06-26 2017-01-11 上海杰隆生物工程股份有限公司 Feeding method of live pigs without using feed antibiotics
CN111789192A (en) * 2020-06-28 2020-10-20 天津瑞益瑞美生物技术有限公司 Functional additive for improving reproductive performance of breeding pigs and preparation method thereof
CN111772046A (en) * 2020-07-14 2020-10-16 江西大佑农生物科技有限公司 Reinforced big pig compound premix feed

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
毕晓华等: "非洲猪瘟疫情下维生素在猪饲料中的应用", 《中国畜牧杂志》 *
陈辉: "菊粉的生物学功能及其在养猪生产中的应用", 《广东饲料》 *

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