CN117210379B - Enterococcus faecium JYEF-355 for promoting cat to change hair and discharge hair and application - Google Patents
Enterococcus faecium JYEF-355 for promoting cat to change hair and discharge hair and application Download PDFInfo
- Publication number
- CN117210379B CN117210379B CN202311482499.XA CN202311482499A CN117210379B CN 117210379 B CN117210379 B CN 117210379B CN 202311482499 A CN202311482499 A CN 202311482499A CN 117210379 B CN117210379 B CN 117210379B
- Authority
- CN
- China
- Prior art keywords
- hair
- enterococcus faecium
- jyef
- cat
- promoting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000282326 Felis catus Species 0.000 title claims abstract description 89
- 210000004209 hair Anatomy 0.000 title claims abstract description 70
- 241000194031 Enterococcus faecium Species 0.000 title claims abstract description 40
- 230000001737 promoting effect Effects 0.000 title claims abstract description 15
- 230000008859 change Effects 0.000 title claims description 6
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 claims description 15
- 239000002068 microbial inoculum Substances 0.000 claims description 15
- 230000000813 microbial effect Effects 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 5
- 235000020183 skimmed milk Nutrition 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 230000003213 activating effect Effects 0.000 claims description 3
- 241001052560 Thallis Species 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 13
- 230000036541 health Effects 0.000 abstract description 11
- 230000002496 gastric effect Effects 0.000 abstract description 9
- 210000001015 abdomen Anatomy 0.000 abstract description 8
- 206010004542 Bezoar Diseases 0.000 abstract description 7
- 230000013872 defecation Effects 0.000 abstract description 6
- 238000000354 decomposition reaction Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 238000009629 microbiological culture Methods 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 238000004088 simulation Methods 0.000 abstract description 4
- 241000194033 Enterococcus Species 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 210000003608 fece Anatomy 0.000 description 10
- 239000000243 solution Substances 0.000 description 7
- 230000008673 vomiting Effects 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 206010047700 Vomiting Diseases 0.000 description 6
- 230000002354 daily effect Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 230000002550 fecal effect Effects 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- 201000004384 Alopecia Diseases 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 208000024963 hair loss Diseases 0.000 description 4
- 230000003676 hair loss Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000006996 mental state Effects 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 230000004580 weight loss Effects 0.000 description 4
- 206010010774 Constipation Diseases 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000003187 abdominal effect Effects 0.000 description 3
- 230000036528 appetite Effects 0.000 description 3
- 235000019789 appetite Nutrition 0.000 description 3
- 206010061428 decreased appetite Diseases 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 210000002268 wool Anatomy 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 206010024642 Listless Diseases 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 208000022531 anorexia Diseases 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 231100000640 hair analysis Toxicity 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 208000017971 listlessness Diseases 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 229940099596 manganese sulfate Drugs 0.000 description 2
- 239000011702 manganese sulphate Substances 0.000 description 2
- 235000007079 manganese sulphate Nutrition 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 2
- 239000001393 triammonium citrate Substances 0.000 description 2
- 235000011046 triammonium citrate Nutrition 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 206010000060 Abdominal distension Diseases 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 208000020401 Depressive disease Diseases 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 206010070245 Foreign body Diseases 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 208000032140 Sleepiness Diseases 0.000 description 1
- 206010041349 Somnolence Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 208000027697 autoimmune lymphoproliferative syndrome due to CTLA4 haploinsuffiency Diseases 0.000 description 1
- 235000014590 basal diet Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000002650 habitual effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000000077 insect repellent Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 201000003102 mental depression Diseases 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 230000036559 skin health Effects 0.000 description 1
- 230000037321 sleepiness Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004206 stomach function Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 210000004916 vomit Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides enterococcus faecium JYEF-355 for promoting cat hair removal and application thereof, belonging to the technical field of microorganisms; the enterococcus faecium JYEF-355 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.27222 at the time of 2023, 4 and 27, and is classified and named as enterococcus faeciumEnterococcus faeciumThe method comprises the steps of carrying out a first treatment on the surface of the Application of enterococcus faecium JYEF-355 in preparing cat hair-setting promoting product. The enterococcus faecium JYEF-355 can promote the decomposition of cat hair in a gastrointestinal simulation environment; the enterococcus faecium JYEF-355 can help cats discharge hair balls in the bellies in a defecation mode, has the effect of promoting hair discharge, and can prevent or treat hair ball diseases; the enterococcus faecium has effect in improving health condition of cat.
Description
Technical Field
The invention relates to enterococcus faecium JYEF-355 for promoting cat hair removal and application thereof, and belongs to the technical field of microorganisms.
Background
Since cats are habitual to licking Mao Shumao and have many barbs on the tongue, hair can not be spit out and can be rolled into the abdomen and gradually clustered into a small sphere. Some of these undigested hairs are expelled with the feces, and some are expelled from the mouth in the form of vomiting. However, a great amount of nondigestible hair is involved in the digestive tract of the cat and when a great amount of hair gathers to form a bulb which is difficult to discharge, the intestinal tract is blocked, so that the cat has symptoms of mental depression, inappetence, sleepiness, vomiting, abdominal distension, abdominal pain, constipation and the like, and the healthy growth of the cat is seriously affected, if the cat is not treated in time, the cat life is even threatened.
In order to prevent or treat hair bulb diseases and help cats discharge hair from the body, hair bulb products such as hair melting paste, hair melting chips, catfish and the like need to be fed to the cats regularly to assist the cats in discharging hair bulbs from the body.
The hair-dissolving paste mainly enables hair to slide out of the alimentary canal through the lubrication action of grease, simultaneously can also cause nutrient substances to slide out together, is easy to cause malnutrition of cats, affects the absorption of nutrient substances by intestines and stomach after long-term eating, and can possibly cause diarrhea; the hair-dissolving tablet contains a large amount of food-calling components and filling agents, has few effective components, can cause a cat to ingest a large amount of unnecessary filling agents when being eaten frequently, causes a certain burden on intestines and stomach, causes the weakening of stomach function when being eaten for a long time, and is not beneficial to the health of the cat; the catgrass is not convenient enough to obtain, the palatability is general, some cats do not like to eat plants, the problems of insufficient feed intake, poor hair removal effect and the like can be caused, and the catgrass mainly promotes the cats to discharge hair balls in a vomit mode, so that painless hair removal cannot be achieved.
Probiotics are microorganisms existing in the intestinal tracts of cats and beneficial to the health of the cats, are applied to the directions of treating diarrhea, improving soft stool, improving immunity and the like of the cats, but strains capable of helping the cats to convert hair and discharge hair are not reported at present.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention provides enterococcus faecium JYEF-355 for promoting cat to change hair and discharge hair and application thereof, and the following aims are achieved: promoting cat hair decomposition; the cat is helped to discharge the hairballs in the bellies in a defecation mode; improving the health condition of cats.
In order to solve the technical problems, the invention adopts the following technical scheme:
enterococcus faecium JYEF-355 for promoting cat to make hair discharge, wherein the enterococcus faecium JYEF-355 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 27222 and classified as enterococcus faecium in the year 2023, month 4 and day 27Enterococcus faecium；
A microbial inoculum prepared from the enterococcus faecium JYEF-355;
the enterococcus faecium JYEF-355 content in the microbial inoculum is 1.4-1.6X10 10 cfu/g；
The preparation method of the microbial inoculum comprises the steps of activating and culturing enterococcus faecium JYEF-355 to obtain microbial inoculum, centrifuging, collecting thalli, re-suspending in reconstituted skim milk to obtain microbial suspension, freeze-drying to obtain microbial powder, and mixing with glucose to obtain the microbial inoculum of enterococcus faecium;
in the bacterial suspension, the concentration of the enterococcus faecium JYEF-355 is 1.8-2.2X10 10 cfu/mL；
The mass concentration of the reconstituted skim milk is 14-16%;
application of enterococcus faecium JYEF-355 in preparing cat hair-setting promoting product.
The enterococcus faecium JYEF-355 was deposited in China general microbiological culture Collection center, address: beijing, chaoyang area, north Chenxi way No.1, no. 3, post code: 100101, accession number: CGMCC No.27222; the strain has effect in promoting cat hair formation and hair removal.
Compared with the prior art, the invention has the following beneficial effects:
the enterococcus faecium JYEF-355 can promote the decomposition of cat hair in a gastrointestinal simulation environment; the enterococcus faecium JYEF-355 can help cats discharge hair balls in the bellies in a defecation mode, has the effect of promoting hair discharge, and can prevent or treat hair ball diseases; the enterococcus faecium JYEF-355 can improve the health condition of cats, help the cats to relieve symptoms such as vomiting, anorexia, camping, constipation, listlessness, hair loss, rough and matt hair caused by accumulation of hair bulbs in the body, and improve scoring values of eight aspects such as stool scoring, vomiting degree, mental state, hair loss degree, hair smoothness, appetite, activity and abdominal state.
Drawings
FIG. 1 is a bar graph of hair output in cat feces;
* Shows significant difference compared with the control groupP< 0.05); * Shows that the difference is very significant compared with the control groupP＜0.001）；
FIG. 2 is a graph showing the effect of enterococcus faecium JYEF-355 on the body condition of cats.
Detailed Description
Example 1 isolation and identification of species
1. Isolation and purification of strains
(1) Bacterial strain source: healthy cat feces were collected from Yi Ling City, shandong, yishui county Chai Yangcun, 9 months and 20 days 2022.
(2) Preparation of MRS solid Medium: 10g of peptone, 10g of beef extract, 5g of yeast extract powder, 20g of glucose, 5g of sodium acetate, 2g of triammonium citrate, 2g of dipotassium hydrogen phosphate, 0.58g of magnesium sulfate, 0.25g of manganese sulfate, 80 1mL of tween-80 and 15g of agar powder are taken, deionized water is used for dissolving, the volume is fixed to 1000mL, high-pressure steam sterilization is carried out for 20min at 115 ℃, and the sterilized culture medium is poured into a plate for stand-by after cooling and solidification.
(3) Isolation and purification of strains: placing 10mL of sterile physiological saline (0.85%) into a sterile triangular flask, adding 1g of the healthy cat feces obtained in the step (1) into the sterile triangular flask, shaking and mixing uniformly, and performing gradient dilution with the sterile physiological saline to obtain a concentration gradient of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Is a diluent of (a). The dilutions were spread onto MRS solid medium plates, respectively, and placed in an anaerobic incubator for stationary culture at 37℃for 48h. And after the culture is finished, selecting colonies with neat edges, a bump in the middle of the bluish white color and a diameter of 1-2 mm. Picking single colony in MRSThree-region streaking is carried out on the body plate, the culture is carried out for 48 hours at 37 ℃ under anaerobic condition, the operation is repeated, 3 times of streaking are carried out, single bacterial colony is selected, and the single bacterial colony is placed in a glycerol tube for preservation at-80 ℃.
2. Authentication
And (3) delivering the single colony separated and purified in the step (3) to China center for identification by a China center for type culture Collection of microorganisms, wherein the gene sequence of the identified 16S rDNA is shown as SEQ ID NO.1 in a sequence table.
The strain is identified as enterococcus faeciumEnterococcus faeciumThe identified strain is named enterococcus faecium JYEF-355 and is sent to China general microbiological culture collection center (CGMCC) for preservation, and the preservation information is as follows:
strain name: enterococcus faecium JYEF-355; classification naming: enterococcus faeciumEnterococcus faeciumThe method comprises the steps of carrying out a first treatment on the surface of the Preservation date: 2023, 4, 27; address: beijing, chaoyang area, north Chenxi way No.1, no. 3, post code: 100101; preservation number: CGMCC No.27222.
EXAMPLE 2 preparation of enterococcus faecium JYEF-355 microbial agent
(1) Preparation of MRS liquid medium: taking 10g of peptone, 10g of beef extract, 5g of yeast extract powder, 20g of glucose, 5g of sodium acetate, 2g of triammonium citrate, 2g of dipotassium hydrogen phosphate, 0.58g of magnesium sulfate, 0.25g of manganese sulfate and 80 1mL of tween-80, dissolving with deionized water, sterilizing with high-pressure steam at 115 ℃ for 20min, pouring the sterilized culture medium into a plate, and cooling for later use.
(2) Preparing bacterial liquid: activating enterococcus faecium JYEF-355 preserved at-80deg.C on MRS solid culture medium plate, selecting 1 single colony after activation, transferring into 100mL MRS liquid culture medium, standing at 37deg.C for 12 hr to obtain seed solution, inoculating the seed solution into MRS liquid culture medium according to 1% (v/v) inoculum size, standing at 37deg.C for 48 hr under anaerobic condition to obtain a large amount of bacterial solution with bacterial concentration of 2.0X10 9 cfu/mL。
(3) Preparing a microbial inoculum: centrifuging the bacterial liquid, collecting bacterial cells, washing with sterile physiological saline, and re-suspending in 15% (w/w) reconstituted skim milk to obtain a concentration of 2.0×10 10 The cfu/mL bacterial suspension is frozen and dried to obtain bacterial powder; mixing the fungus powder with glucose to prepare a fungus agent; glucose was purchased from shan east king sugar industry limited; in this example, the number of cells in the microbial inoculum prepared was 1.5X10 10 cfu/g。
Example 3 in vitro bionic digestion test of cat hair
1. Test materials: 3 cats with good skin health conditions (the varieties are respectively puppets, silver gradual layers and short, and the cat hair is collected by 1g of each cat) and placed in a 50mL centrifuge tube. And taking out the cat hair, and drying the cat hair in an oven at 65 ℃ until the weight is constant.
2. Preparation of simulated gastric fluid and simulated intestinal fluid
(1) Preparation of simulated gastric fluid: weighing 0.2g of sodium chloride and 0.32g of pepsin, adding 100mL of distilled water, stirring for dissolution, adjusting the pH to 2.5 by using 1mol/L hydrochloric acid solution, and filtering with a 0.2 mu m filter membrane for later use.
(2) Preparation of simulated intestinal juice: 0.68g of potassium dihydrogen phosphate was weighed, dissolved in 50mL of distilled water, and pH was adjusted to 6.75 with 0.1mol/L sodium hydroxide solution. 1g of pancreatin is weighed, and 50mL of distilled water is added for stirring and dissolution. Mixing the above two solutions to obtain simulated intestinal juice.
3. The test method comprises the following steps:
(1) 6 identical beakers (3 each for the experimental group and the control group) were taken, 0.3g of the cat hair after constant weight in step 1 (0.1 g each for 3 cat hairs) was added to each beaker, 20mL of the bacterial liquid prepared in step (2) of example 2 was added to the experimental group, and 20mL of the sterile MRS liquid medium was added to the control group.
(2) 50mL of simulated gastric fluid was added to each beaker, shaken well, and placed on a shaker to simulate gastric digestion for 4h (60 rpm at 37 ℃). The gastric simulation product was removed, pH was adjusted to 6.75 using 1mol/L sodium hydroxide solution, 40mL of simulated intestinal fluid was added, sealing was performed, and the shaker intestinal simulated digestion was allowed to stand for 9h (60 rpm at 37 ℃), after which the product was removed after completion of the gastrointestinal simulation.
(3) The cat hair was filtered through an 80 mesh nylon mesh, washed three times with distilled water, and dried in an oven at 65 ℃ to constant weight.
(4) The weight of cat hair after constant weight was weighed, and the weight loss ratio was calculated, and the results are shown in Table 1.
TABLE 1 weight loss of cat hair
Note that: * Shows significant difference compared with the control groupP＜0.05）。
As can be seen from Table 1, the weight loss rate of cat hair increased after the enterococcus faecium JYEF-355 bacterial liquid was added. The weight loss rate of the cat hair test group is improved by 5.43 percent compared with that of the control group, and the cat hair test group has obvious change. The enterococcus faecium JYEF-355 can promote the decomposition of cat hair in the gastrointestinal simulated environment, and has a strong promoting effect on the cat hair decomposition.
EXAMPLE 4 enterococcus faecium JYEF-355 in vivo hairing test
(1) The test selects 20 healthy adult puppet cats with the weight of 3.5-4.5 kg, the weight is randomly divided into 2 groups (test group and control group), each group is 10 repeated, each group is repeated by 1 cat (each group comprises 1/2 of male and female cats), and all cats do not take any fur-dissolving product within half a year before the test starts.
(2) Necessary immunization and insect repellent treatment were performed before the test, the cat litter was replaced daily, and the cat house was cleaned daily.
(3) During the test period, each cat was fed in a single cage, the control group and the test group were fed with the same amount of basal diet daily, the control group was supplemented with 2g of glucose daily, and the test group was supplemented with 2g of the microbial inoculum prepared in example 2 daily.
(4) The pre-test period is 1 week, the positive test period is 8 weeks, on the 7 th day, the 14 th day, the 21 st day, the 28 th day, the 35 th day, the 42 th day, the 49 th day and the 56 th day, all the feces of the cat on the day are collected, whether the cat hair is discharged or not is observed, the cat hair in the feces is separated, the cat hair is dried to constant weight in a drying box at 65 ℃ after being washed by clear water, and the data are weighed and recorded, and the result is shown in figure 1.
As can be seen from fig. 1, during the test period, the fecal hair output of the cats in the test group tended to rise and then fall and then tended to be gentle, whereas the fecal hair output of the cats in the control group did not change much throughout the process. On test day 7, the cats in the test group discharge a large amount of hair through defecation, the weight of the hair bulb in the feces is increased until day 21, and the weight of the hair bulb in the feces of the cats in the control group is stable, which means that a large amount of hair bulb is accumulated in the abdomen of the cats, and if no product which is helpful for hair melting is taken, a small amount of hair can be discharged through defecation every day, and the intestinal tract remains, so that the health is influenced over time. After the microbial inoculum prepared in the example 2 is taken for a period of time, the hair discharge amount in the cat feces of the test group is obviously higher than that of the control group, which indicates that the microbial inoculum is helpful for cats to discharge hair balls in the bellies in a defecation mode, has the effect of promoting hair discharge, takes effect quickly and is beneficial to health. On day 28, the weight of the fecal hair bulb began to decrease in the test group, and after day 42, the fecal hair output tended to stabilize, probably by having previously discharged most of the hair bulb accumulated in the abdomen, but since cats were habituated to lick Mao Shumao, hair was constantly being discharged, and the fecal hair output of the test group was always higher than that of the control group during the test. Since cats in the control group do not take the wool-dissolving effect product for a long time, only a small amount of wool balls can be discharged daily, a large amount of wool balls are left in the abdomen, and the wool balls cannot be digested and cannot be discharged. On day 60, 20 cats were palpated by a professional feline doctor, more than half of the cats in the control group had soft foreign bodies in their small intestines, and were subsequently treated.
Example 5 test of the Effect of enterococcus faecium JYEF-355 on the body Condition of cats
When a large amount of hair gathers or has globus fulgidus in the cat digestive tract, the cat is often accompanied by symptoms such as vomiting, anorexia, camping, constipation, listlessness, hair loss, rough hair, no luster and the like. Cat health assessment criteria (table 2) were formulated based on the above symptoms and the cats in example 4 were scored for health by a professional assessor for a period of time of day 56 of example 4, with the assessment results shown in figures 2 and 3.
TABLE 2 Cat health assessment criteria
TABLE 3 enterococcus faecium JYEF-355 influence on cat physical condition scoring results
As can be seen from fig. 2 and table 3, the test group has higher score values in terms of feces, vomiting degree, mental state, hair loss degree, hair smoothness, appetite, activity and abdominal state than the control group, which indicates that the enterococcus faecium JYEF-355 microbial agent has an improving effect on the health condition of cats. Wherein, the method is particularly prominent in improving the mental state of cats, and the scores of cats in the test group on the mental state are significantly different from those of cats in the control groupP<0.001). In addition, the cat in the test group had the hair falling degree [ ]P=0.039), appetite%P=0.044) and abdominal stateP=0.014) the score values were significantly higher than the control group.
Claims (6)
1. Enterococcus faecium capable of promoting cat to change hair and discharge hairEnterococcus faecium) Bacterial strain JYEF-355 with preservation number of CGMCC No.27222。
2. A microbial inoculum prepared by using the enterococcus faecium strain JYEF-355 as claimed in claim 1.
3. The microbial agent of claim 2, wherein: the enterococcus faecium strain JYEF-355 in the microbial inoculum has the quantity of 1.4-1.6X10 10 cfu/g。
4. The microbial agent of claim 2, wherein: the preparation method of the microbial inoculum comprises the steps of activating and culturing enterococcus faecium strain JYEF-355 to obtain a microbial inoculum, centrifuging, collecting thalli, re-suspending in recovered skim milk to obtain a microbial suspension, freeze-drying to obtain microbial powder, and mixing with glucose to obtain the microbial inoculum.
5. The microbial agent of claim 4, wherein: in the bacterial suspension, the concentration of the enterococcus faecium strain JYEF-355 is 1.8-2.2X10 10 cfu/mL; the mass concentration of the reconstituted skim milk is 14-16%.
6. Use of the enterococcus faecium strain JYEF-355 according to claim 1 for preparing a microbial agent for preventing or treating chaetosis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311482499.XA CN117210379B (en) | 2023-11-09 | 2023-11-09 | Enterococcus faecium JYEF-355 for promoting cat to change hair and discharge hair and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311482499.XA CN117210379B (en) | 2023-11-09 | 2023-11-09 | Enterococcus faecium JYEF-355 for promoting cat to change hair and discharge hair and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117210379A CN117210379A (en) | 2023-12-12 |
| CN117210379B true CN117210379B (en) | 2024-01-30 |
Family
ID=89044791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311482499.XA Active CN117210379B (en) | 2023-11-09 | 2023-11-09 | Enterococcus faecium JYEF-355 for promoting cat to change hair and discharge hair and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117210379B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101968243B1 (en) * | 2018-11-30 | 2019-04-11 | 주식회사 아미코스메틱 | Enterococcus faecium ami-1110 and use thereof |
| CN109673861A (en) * | 2018-11-12 | 2019-04-26 | 山东艾贝迪宠物食品有限公司 | A kind of cat algal fungi nutrient laden hair cream and preparation method thereof |
| CN114223788A (en) * | 2021-12-29 | 2022-03-25 | 安徽爱不停蛋白质饲料有限公司 | Complex enzyme preparation for effectively removing hair bulbs in pet bodies and application thereof |
| CN114540239A (en) * | 2022-03-10 | 2022-05-27 | 浙江大学 | Rabbit-derived enterococcus faecium ZJuIDS-R1 for preventing pet diarrhea and application thereof |
| KR102531190B1 (en) * | 2022-06-28 | 2023-05-11 | 일동바이오사이언스(주) | Composition for preventing or treating obesity of companion animal comprising Enterococcus faecium IDCC 2102 |
| CN116640705A (en) * | 2023-07-21 | 2023-08-25 | 山东威曼宠物食品有限公司 | Lactobacillus acidophilus King45 for improving pet hair, and microbial inoculum and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112022026478A2 (en) * | 2020-06-29 | 2023-03-07 | Canbiocin Inc | RELATED COMPOSITIONS AND METHODS FOR SUPPORTING DOMESTIC ANIMALS WITH GASTROINTESTINAL DISORDERS |
-
2023
- 2023-11-09 CN CN202311482499.XA patent/CN117210379B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109673861A (en) * | 2018-11-12 | 2019-04-26 | 山东艾贝迪宠物食品有限公司 | A kind of cat algal fungi nutrient laden hair cream and preparation method thereof |
| KR101968243B1 (en) * | 2018-11-30 | 2019-04-11 | 주식회사 아미코스메틱 | Enterococcus faecium ami-1110 and use thereof |
| CN114223788A (en) * | 2021-12-29 | 2022-03-25 | 安徽爱不停蛋白质饲料有限公司 | Complex enzyme preparation for effectively removing hair bulbs in pet bodies and application thereof |
| CN114540239A (en) * | 2022-03-10 | 2022-05-27 | 浙江大学 | Rabbit-derived enterococcus faecium ZJuIDS-R1 for preventing pet diarrhea and application thereof |
| KR102531190B1 (en) * | 2022-06-28 | 2023-05-11 | 일동바이오사이언스(주) | Composition for preventing or treating obesity of companion animal comprising Enterococcus faecium IDCC 2102 |
| CN116640705A (en) * | 2023-07-21 | 2023-08-25 | 山东威曼宠物食品有限公司 | Lactobacillus acidophilus King45 for improving pet hair, and microbial inoculum and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| Heat-Killed Enterococcus faecalis EF-2001 Induces Human Dermal Papilla Cell Proliferation and Hair Regrowth in C57BL/6 Mice;Baek YH等;《Int J Mol Sci》;第23卷(第10期);第1-13页 * |
| 屎肠球菌QH06能减轻溃疡性结肠炎大鼠的结肠黏膜损伤;库尔班乃木•卡合曼等;《南方医科大学学报》;第42卷(第07期);第976-987页 * |
| 屎肠球菌制剂对老龄宠物犬血脂代谢、抗炎及抗氧化能力的影响;朱道仙等;《黑龙江畜牧兽医》;第2020卷(第11期);第146-149页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117210379A (en) | 2023-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103497906B (en) | Microecological preparation, preparation method, and applications thereof | |
| CN106509439B (en) | The feed addictive of substitute antibiotics preventing post-weaning diarrhea | |
| CN104774776B (en) | The Bu Ladi saccharomycete of one plant of high-temp resisting high-humidity resisting and its application | |
| US20160038547A1 (en) | Lactobacillus plantarum capsule for poultry and use thereof | |
| CN105368749B (en) | One bacillus subtilis and feed addictive comprising the bacterial strain | |
| CN109601734A (en) | Feed addictive containing procyanidine and dietary fiber and preparation method thereof and nonreactive pig starter feed product | |
| CN114774313A (en) | Application of lactobacillus rhamnosus LRa05 in preparing product for relieving constipation or regulating intestinal flora | |
| CN106417989A (en) | Probiotic composition for aquatic animal feed, preparation method and application thereof | |
| CN102618452B (en) | Preparation method, composition and application of lactobacillus salivarius and its metabolites | |
| CN110317753A (en) | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application | |
| KR102111669B1 (en) | Special medium for mass manufacturing a complex of probiotics, and method for mass manufacturing the complex of probiotics using it | |
| CN105029031A (en) | Method for improving growth performance of weaned pigs and reducing diarrhea rate through coprophilous fungus transplantation | |
| CN106721052B (en) | Feed produced by using orange peel dregs after pectin extraction and production process thereof | |
| CN109486732B (en) | Bifidobacterium longum and application thereof | |
| CN109561711B (en) | Methods of treating digestive tract disorders | |
| CN104830711B (en) | A kind of solid fermentation process of bacillus amyloliquefaciens feed addictive | |
| CN101999556A (en) | Special animal nutrient for beef cattle and preparation method thereof | |
| CN116855413B (en) | Bioactive substance for regulating human body microecological balance prepared from lactobacillus rhamnosus YSs069 and application thereof | |
| CN110089639A (en) | A kind of feed addictive improving child care pig growth performance and its preparation and application | |
| CN113616715A (en) | Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora thereof | |
| CN108902445A (en) | A kind of improvement intestine of young pigs health additive and preparation method thereof | |
| CN108783115A (en) | A kind of immunopotentiator and the preparation method and application thereof preventing red head disease of Pseudobagrus fulvidraco | |
| CN101228920B (en) | Application of microecologics prepared from bacillus laterosporus WY9701 on feed additive | |
| CN117210379B (en) | Enterococcus faecium JYEF-355 for promoting cat to change hair and discharge hair and application | |
| CN115895966B (en) | Bifidobacterium bifidum BL002 for assisting in relieving gout and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |