KR101968243B1 - Enterococcus faecium ami-1110 and use thereof - Google Patents

Abstract

The present invention relates to a strain of Enterococcus faecium AMI-1110 (KCCM12386P); and a cosmetic composition and a food composition comprising the same. Unlike conventional synthetic antibacterial substances causing skin irritations and cosmetic composition comprising the same, the composition comprising the strain of Enterococcus faecium AMI-1110 (KCCM12386P) of the present invention has excellent antibacterial activities, and particularly, has excellent antibacterial activities against bacteria causing acne and atopic dermatitis.

Description

Enterococcus faecium AMI-1110 strain and its use {ENTEROCOCCUS FAECIM AMI-1110 AND USE THEREOF}

The present invention relates to Enterococcus faecium AMI-1110 KCCM12386P strain, a cosmetic composition containing the same, and a food composition.

Human skin is an organ that makes direct contact with the surrounding environment, and infections such as bacteria and fungi that are involved in various skin diseases are easily caused. For example, the stapling Philo Staphylococcus aureus causing maturation skin nose kusu aureus (Staphylococcus aureus) the person, including the skin flora of Stability Philo nose kusu epidermidis (Staphlyococcus epidermidis), acne bacteria Propionibacterium sludge tumefaciens arc Ness (Propionibacterium acnes ) are present in the skin. The bacteria degrade the sebum or sweat secreted on the skin to generate an odor. In addition, the degradation products stimulate the skin and cause inflammation. Propionibacterium acnes , in particular, uses lipase, a fatty acid degrading enzyme, to decompose sebum to produce free fatty acids. These free fatty acids not only stimulate the skin but also cause inflammatory lesions such as papules, pustules, and nodules, which are red rashes found in acne.

Antimicrobial compounds such as triclocarban and triclosan have been used as antimicrobial agents against such bacteria, but they are known to cause side effects such as skin burning, burning, and skin redness during long-term use.

As described above, the conventional antimicrobial synthetic material and the cosmetic composition containing the antimicrobial material have a risk of causing skin irritation and require a new composition of antimicrobial substance in that they can cause skin troubles.

Korea Patent No. 10-1841090

It is an object of the present invention to provide Enterococcus faecium AMI-1110 KCCMKCCM12386P strain.

Another object of the present invention is to provide a cosmetic composition comprising the above strain, a biomass thereof, a bacterium thereof, a culture thereof, a disruption thereof or an extract thereof.

It is still another object of the present invention to provide a food composition comprising the strain, a live cell thereof, a bacterium thereof, a culture thereof, a lysate thereof or an extract thereof.

In order to accomplish the above object, an aspect of the present invention relates to Enterococcus faecium AMI-1110 KCCM12386P strain.

The 16S rDNA nucleotide sequence for the identification and classification of the Enterococcus pathum AMI-1110 of the present invention is shown in SEQ ID NO: 1 attached hereto. Thus, the Enterococcus faecium AMI-1110 of the present invention may comprise the 16S rDNA of SEQ ID NO: 1.

As a result of analysis of the 16S rDNA sequence of SEQ ID NO: 1, 99% homology with the known Enterococcus paclitus strains showed the highest molecular phylogenetic relationship with the Enterococcus pathum. Thus, the strain was identified as Enterococcus faecium , designated Enterococcus lacium AMI-1110, and deposited on Nov. 12, 2018 (Accession No. KCCM 12386P) at the Korean Microorganism Conservation Center.

In the present invention, the term " culture product " or " culture medium " means an object obtained by culturing the novel strain of the present invention in a known liquid medium or solid medium. In the present invention, a novel Enterococcus paclility AMI- .

In the present invention, " antibacterial " refers to all actions that inhibit bacteria or fungi, and in particular may mean inhibition or elimination of bacteria or fungi harmful to the human body.

In one embodiment of the present invention, when a mixture or a cosmetic composition containing a cultured strain of Enterococcus faecium AMI-1110 KCCM 12386P was applied, antimicrobial activity exhibiting suppression of bacteria residing in the skin was exhibited (see Table 5 ), And it was confirmed that acne improvement effect was observed when applied to skin (Table 7). In particular, the antimicrobial activity exhibits antibacterial activity not only against acne-causing bacteria but also against atopy-induced bacteria, and can be utilized for improving acne or atopy.

Specifically, the antimicrobial activity may be selected from the group consisting of Micrococcus luteus , Staphyrococcus aureus KCTC 3881, Candida albicans KCTC 7965, Propionibacterium acnes ATCC 6919 ), Staphylococcus epidermidis ATCC 12228, Malassezia furfur ATCC 14521, and Proteus rettgeri ATCC 29944. The antimicrobial activity may be at least one selected from the group consisting of Staphylococcus epidermidis ATCC 12228, These microorganisms are also known as causative microorganisms or atopy causative microorganisms. The microorganism of the present invention, the microbial cells of the present invention, the microbial cells thereof, the microbial cells thereof, the microbial cells thereof, the disruption products thereof or the extracts thereof, It can also be used for atopy improvement.

In the present invention, " acne " is caused by an increase in the production of sebum in the pores, abnormal erosion of the skin epithelium, blocking the opening of the pore, and proliferation of bacteria causing acne and causing inflammation. By inhibiting the bacteria that cause acne, it is possible to prevent the clogging of pores and the excessive secretion of sebum, thereby improving acne.

In the present invention, "atopy" refers to an allergic reaction in which the body becomes extremely sensitive without direct contact with allergic antigens. The cause of the allergic reaction is not specifically disclosed, and the family history, sex, body mass index, , Pets, birth order, socioeconomic factors, and so on. Steroids, which are currently used as atopic treatments, are reported to have side effects, and development of natural substances to reduce side effects is required.

The cosmetic composition of the present invention can be used as a cosmetic composition in the form of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening lotion, a pack, a soft water, a latex, a makeup base, But are not limited to, those selected from the group consisting of solutions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powdered foundations, emulsion foundations, wax foundations, patches and sprays no.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

Specifically, in the above-mentioned cosmetic composition, the strain, the cells thereof, the cells thereof, the culture thereof, the disruption thereof or the extract thereof may be contained in an amount of 0.005 to 30.0% by weight based on the total weight of the cosmetic composition. And more specifically from 0.01% to 20% by weight. In addition to excellent antimicrobial activity within the above range, it has an advantage of improving acne and atopy, and has an advantage that the formulation of the composition is stabilized.

In addition, the composition of the present invention can be used as a transdermal administration method such as direct application or spraying on the skin, and the administration route of the composition of the present invention can be administered through any ordinary route as long as it can reach the target tissue.

The amount of the composition of the present invention can be appropriately adjusted according to individual differences such as age, degree of lesion, and the like, and can be used for one week to several months after once to several times daily application.

In order to achieve the above object, another aspect of the present invention relates to a food composition comprising the strain, the biomass thereof, the biomass thereof, the culture thereof, the degradation product thereof or the extract thereof. Specifically, the food composition may be characterized by exhibiting antibacterial activity, but is not limited thereto, and may be used for acne or atopic amelioration.

The terms "antibacterial", "acne", "atopy" and the like are as described above.

There is no particular limitation on the kind of the food. Foods to which the Enterococcus faecium AMI-110 can be added include dairy products including sausages, meat, bread, chocolates, snacks, candies, confectionery, ramen, pizza and other noodles, gums, ice cream, , Tea, a drink, an alcoholic beverage, and a vitamin complex. In the case of formulation with beverage, the liquid ingredient to be added in addition to the Enterococcus passif AMI-1110 may include various flavors or natural carbohydrates such as ordinary beverages as an additional ingredient. The above-mentioned natural carbohydrates may be used in combination with monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose and polysaccharides such as dextrins and cyclodextrins and xylitol and sorbitol , Erythritol, and the like.

The type of the food may be specifically a health functional food. The health functional food may contain flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, organic acids, A pH adjuster, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, and the like. In addition, the health functional food of the present invention may contain pulp for the production of fruit and vegetable drinks. These components may be used singly or in combination, and the proportion of such additives is generally selected in the range of 0.001 to 50 parts by weight based on the total weight of the composition.

The health functional food is a food emphasizing the biological control function of food, and is added with added value so as to function and manifest to a specific purpose using physical, biochemical and biotechnological methods. These health functional food ingredients are designed and manufactured so that the body control functions related to regulation of body defense and body rhythm, prevention and recovery of disease are sufficiently exhibited to the living body, and food additives, And may contain raw materials.

When the Enterococcus lacZ AMI-1110 of the present invention is used as a health functional food (or a health functional beverage additive), the enterococcus lacZ AMI-1110 may be directly added or used together with other food or food ingredients, And the like. The mixing amount of the Enterococcus faecium AMI-1110 may suitably be determined according to its use purpose (prevention, health or improvement, therapeutic treatment).

The Enterococcus faecium AMI-1110 KCCM12386P strain or composition comprising the same of the present invention has excellent antimicrobial activity, and particularly has excellent antimicrobial activity against acne and atopy causative bacteria.

In addition, the composition of the present invention is free of toxicity and does not cause adverse effects even when it is applied to human body, so that it is highly utilized as a cosmetic composition and a food composition.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1 Preparation and Identification of Enterococcus Pacem AMI-1110

1-1. Separation from the salted cod

The salted salted salted fish was crushed and homogenized, and 1.0 g of the salted salted salted fish was suspended in 9.0 ml of sterilized physiological saline (0.85% NaCl), diluted stepwise, and sprouted on each of the MRS growth medium and the respective selective media shown in Table 1 below. After culturing at 37 ° C for 24 to 48 hours, strains with different colonies were selected and purified by pure culture several times. The pure isolates were plated on MRS solid medium and cultured at 37 ° C for 24 hours. The pure colonies were cultured in MRS broth and mixed with 1% (v / v) glycerol of 20% (v / v) And stored at -80 ° C in a cryogenic freezer.

Strain Optional badge Propagation medium Lactobacillus sp. Strain
Lactobacillus spp. LBS
(Lactobacillus selection) MRS Lactococcus sp.
Lactococcus spp. M17 MRS Pediococcus sp.
Pediococcus spp. PSM MRS Strain of Ryukonosu
Leuconostoc spp. PES-30 MRS Enterococcus spp. Enterococcus spp. LBS MRS

1-2. Identification of Enterococcus pathum AMI-1110 strain

The isolated strains were identified by 16S rDNA sequencing. Genomic DNA of isolate strain was extracted with DNA using ZR Bacterial DNA MiniPrepTMkit (ZYMORESEARCH) and used as a template. PCR primers were primers 27F (5'-AGAGTTTGATCMTGGCTCAG-3 ') and 1492R (5'-TACGGYT ACCTTGTT ACGACTT-3') as primers for PCR amplification of 16S rDNA. Min, denaturation at 94 ° C for 45 seconds, annealing at 55 ° C for 60 seconds, and extension at 72 ° C for 60 seconds. The 16S rDNA sequence was verified by Macrogen, Inc., and strains corresponding to the GRAS (Generally Recognized as Safe Substance) were firstly screened.

SEQ ID NO: primer order 2 27F 5'-AGAGTTTGATCMTGGCTCAG-3 ' 3 1492R 5'-TACGGYT ACCTTGTT ACGACTT-3 '

The nucleotide sequences of the strains obtained above were compared with the nucleotide sequences listed in the GenBank database. As a result, the nucleotide sequences of Enterococcus were 99% similar to those of Enterococcus . In addition, compared with the nucleotide sequence of the Enterococcus faecium JCM 8727 strain, the similarity was analyzed to be 99%, and a commonly accepted paper (Stackebrandt & Goebel, 1994 ; Stackebrandt & Ebers, 2006), and was identified as belonging to the genus Lactobacillus plantarum . The identified strain was named Enterococcus faecium AMI-1110, and on November 12, 2018, the Korean microorganism conservation center (Address: 2 Kagiri 45 Jyungim Building, Hongje, Seodaemun- ), And a single colony of Enterococcus faecium AMI-1110 ( Enterococcus faecium AMI-1110), a strain isolated in the above example, was added to each flask. And cultured for 48 hours. Suspension cultures were centrifuged, supernatant was obtained, and the cells were removed by filtration with a 0.22-μm filter. Using the culture broth prepared above, a cytotoxicity test, a paper disc measurement test, an L-DOPA (L-3, 4-Dihydroxyphenylalanine) oxidation inhibitory activity, . As a control, a culture solution of Enterococcus pathum JCM 8727 strain, which showed 99% homology in the 16S rDNA gene sequence, was used, and the same method was also used for preparing the strain culture solution of the control group.

Experimental Example 1. Cytotoxicity test

To verify skin safety, cytotoxicity tests on human fibroblasts were performed. Fibroblasts were plated in 96-well plates at 5 × 10 ³ / well, respectively, and cultured for 24 hours. Then, the prepared culture broth was treated for each concentration, and after 48 hours, the broth containing the culture broth was removed. Then, 10% EZ-Cytox reagent was mixed in DMEM medium, and 100 ul was added per well. After incubation for 2 hours, absorbance was measured at 450 nm wavelength with a microplate reader (Biotek, Synergy HT). Cell viability was then calculated according to equation 1 below.

[Formula 1]

Cell survival rate (%) = (absorbance of the culture medium treated with the strain / absorbance of the culture medium without treatment) X 100

Human fibroblast cell survival rate (%) Concentration (ug / ml) 0 0.5 One 3 5 Enterococcus pathum AMI-1110 strain 100 104.75 101.12 99.28 97.26 Enterococcus Passium JCM 8727
Strain 100 98.15 97.64 81.35 68.41

As shown in Table 3, it was confirmed that the strain of the present invention can be used as a safe material because cytotoxicity is not observed.

Experimental Example 2. Identification of Antibacterial Activity

The antimicrobial activity of the strain of the present invention against skin-resident bacteria was confirmed using a well diffusion assay.

Specifically, micrococcus luteus , Staphyrococcus aureus KCTC 3881, Candida albicans KCTC 7965, Propionibacterium acnes ATCC 6919, staphylococcus aureus, Staphylococcus epidermidis ATCC 12228, Malassezia furfur ATCC 14521 and Proteus rettgeri ATCC 29944 were cultured in NB / MRS liquid medium for 24 hours, followed by centrifugation and filtration to obtain supernatant Were recovered and mixed at a level of 1.0 x 10 ⁶ CFU / ml in 100 ml of NB / YM soft agar (0.8 agar), respectively. The mixed medium solution was dispensed into Petri Dish (SPL, Φ) in 25 ml portions and completely hardened, and then a hole having a diameter of 0.5 to 0.8 cm was formed using a sterilized tip. 80 [mu] l of the culture medium of the AMM-1110 strain, the culture medium of the Enterococcus pathum JCM 8727 strain and the positive control triclosan was allowed to stand at room temperature for 1 hour to allow it to diffuse into the supernatant. For more than 15 hours to confirm the generation of inhibitory rings.

Inhibition ring (cm ² ) Enterococcus Fassium AMI-1110 Enterococcus Passium JCM 8727 Positive control group
(Triclosan) Microcorcus Ruteus 6.1 1.2 4.0 Staphylococcus aureus 7.3 2.1 3.8 Candida Albikans 6.5 1.5 5.1 Probiorni Bacterium Acne 6.8 2.6 4.2 Staphylococcus epidermidis 7.1 1.9 4.1 Marasegia Pullear 7.4 2.3 3.7 Proteus Retgeri 6.8 3.0 3.9

As shown in Table 4, when the Enterococcus lacZ AMI-1110 culture medium of the present invention was applied, it showed excellent antimicrobial activity and was found to be superior to triclonic acid, which is a positive control, in antimicrobial activity.

Particularly, the present invention relates to a method for producing microorganisms such as Micrococcus luteus , Staphyrococcus aureus KCTC 3881, Candida albicans KCTC 7965, Propionibacterium acnes ATCC 6919, Staphylococcus aureus, Staphylococcus epidermidis ATCC 12228, Malassezia furfur ATCC 14521, and Proteus rettgeri ATCC 29944 are known as acne or atopy-causing strains. When the strain of the present invention has acne or atopy The results of this study are as follows.

Experimental Example 3. Identification of acne improvement effect

The cosmetic composition containing the culture solution of the strain of the present invention was tested for improving skin complexion. Thirty women (mean age 35 years) with an acne lesion of 20 years or older under the conditions of a temperature of 24 to 26 DEG C and a humidity of 75% were divided into three groups and coated with the cosmetic composition according to the following Table 5, respectively.

ingredient Experimental Example Positive control group An Enterococcus pathum AMI-1110 culture medium or an Enterococcus pathum JCM 8727 culture medium 10.0 Commercial Acne Cream Cetostearyl alcohol 2.0 Chin type glyceryl stearate 1.5 Glyceryl stearate SE 1.5 Phitosqualan 4.0 Hydrogenated Polydecenes 3.0 Dimethicone 0.5 Polysorbate 60 1.0 Sorbitan sesquioleate 0.5 Methyl paraben 0.1 Propylparaben 0.1 Purified water Balance Butylene glycol 5.0 Polyacrylate-13 0.5 synthesis 100 100

The formulated cosmetic composition was applied for 4 weeks twice a day (morning and evening), and degree of improvement was evaluated according to the following evaluation criteria according to the opinion of the user. The results are shown in Table 6 below.

division 1 week 2 weeks 3 weeks 4 weeks Enterococcus pathum AMI-1110 culture medium + ++ ++ +++ Enterococcus pathum JCM 8727 culture medium ± ± ± + Commercial Acne Cream ± + + ++

As shown in Table 6, when the cosmetic composition comprising the culture medium of Enterococcus faecium AMI-1110 of the present invention was applied, it was confirmed that the degree of improvement of acne was excellent.

Production Example 1. Preparation of an essence containing culture medium of Enterococcus pathum AMI-1110 strain

A cosmetic composition of the essence formulation was prepared as shown in Table 7 below (unit: wt%).

ingredient Content (% by weight) Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 Enterococcus pathum AMI-1110 culture medium 5 Purified water To 100

Production Example 2. Production of cream containing culture medium of Enterococcus pathum AMI-1110 strain

A cosmetic composition of cream form was prepared as shown in Table 8 below (unit: wt%).

ingredient Content (% by weight) Enterococcus pathum AMI-1110 culture medium 5 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Suitable amount incense Suitable amount Purified water to 100

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described in a single form may be distributed and implemented, and components described as being similarly distributed may also be implemented in a combined form. The scope of the present invention is defined in the appended claims And all changes or modifications derived from the meaning and scope of the claims and their equivalents are to be interpreted as being included within the scope of the present invention.

Korea Microorganism Conservation Center (overseas) KCCM12386P 20181112

<110> AMI COSMETIC CO., LTD. <120> ENTEROCOCCUS FAECIUM AMI-1110 AND USE THEREOF <130> 18PP31068 <160> 3 <170> KoPatentin 3.0 <210> 1 <211> 1501 <212> DNA <213> Artificial Sequence <220> <223> Enterococcus faecium AMI-1110 16S rDNA <400> 1 ttcgttcagg acaaacgcgg cggcgtgcct aatacatgca agtcgtacgc ttctttttcc 60 accggagctt gctccaccgg aaaaagagga gtggcgaacg ggtgagtaac acgtgggtaa 120 cctgcccatc agaaggggat aacacttgga aacaggtgct aataccgtat aacaatcgaa 180 accgcatggt tttgatttga aaggcgcttt cgggtgtcgc tgatggatgg acccgcggtg 240 cattagctag ttggtgaggt aacggctcac caaggccacg atgcatagcc gacctgagag 300 ggtgatcggc cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg 360 gaatcttcgg caatggacga aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt 420 cggatcgtaa aactctgttg ttagagaaga acaaggatga gagtaactgt tcatcccttg 480 acggtatcta accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg 540 tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg caggcggttt cttaagtctg 600 atgtgaaagc ccccggctca accggggagg gtcattggaa actgggagac ttgagtgcag 660 aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca 720 gtggcgaagg cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa 780 acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttggagggt 840 ttccgccctt cagtgctgca gctaacgcat taagcactcc gcctggggag tacgaccgca 900 aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat 960 tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct ttgaccactc tagagataga 1020 gcttcccctt cgggggcaaa gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga 1080 gatgttgggt taagtcccgc aacgagcgca acccttattg ttagttgcca tcattcagtt 1140 gggcactcta gcaagactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca 1200 tcatgcccct tatgacctgg gctacacacg tgctacaatg ggaagtacaa cgagttgcga 1260 agtcgcgagg ctaagctaat ctcttaaagc ttctctcagt tcggattgca ggctgcaact 1320 cgcctgcatg aagccggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt 1380 cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg 1440 aggtaacctt tttggagcca gccgcctaag gtgggataga tgattggggt gaagtctaaa 1500 a 1501 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F primer <400> 2 agagtttgat cmtggctcag 20 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 1492R primer <400> 3 tacggytacc ttgttacgac tt 22

Claims (12)

Enterococcus faecium AMI-1110 KCCM12386P strain. The method according to claim 1,
11. The Enterococcus faecium AMI-1110 KCCM12386P strain, wherein said strain comprises the 16S rDNA nucleotide sequence of SEQ ID NO: 1. The Enterococcus faecium AMI-1110 strain KCCM12386P according to claim 1, wherein said strain exhibits antibacterial activity. The method according to claim 3, wherein the antibacterial activity is selected from the group consisting of Micrococcus luteus , Staphyrococcus aureus , Candida albicans , Propionibacterium acnes , Wherein the antimicrobial activity is at least one selected from the group consisting of Staphylococcus epidermidis , Malassezia furfur , and Proteus rettgeri . Enterococcus faecium AMI-1110) KCCM12386P strain. A cosmetic composition comprising a strain according to any one of claims 1 to 4, a live cell thereof, a dead cell thereof, a culture thereof, a lysate thereof or an extract thereof. 6. The cosmetic composition according to claim 5, wherein the cosmetic composition exhibits antibacterial activity. 7. The method according to claim 6, wherein the antimicrobial activity is selected from the group consisting of Micrococcus luteus , Staphyrococcus aureus , Candida albicans , Propionibacterium acnes , Wherein the composition is an antimicrobial activity against at least one selected from the group consisting of Staphylococcus epidermidis , Malassezia furfur , and Proteus rettgeri . 6. The cosmetic composition according to claim 5, wherein the cosmetic composition is used for improving acne or atopy. A food composition comprising the strain of any one of claims 1 to 4, a live cell thereof, a live cell thereof, a culture thereof, a crushed product thereof or an extract thereof. 10. The food composition of claim 9, wherein the food composition exhibits antimicrobial activity. 11. The method of claim 10, wherein the antimicrobial activity is selected from the group consisting of Micrococcus luteus , Staphyrococcus aureus , Candida albicans , Propionibacterium acnes , Wherein the food composition is an antimicrobial activity against at least one selected from the group consisting of Staphylococcus epidermidis , Malassezia furfur , and Proteus rettgeri . 10. The food composition of claim 9, wherein the food composition is an acne or atopy improvement application.