CN113475534B - Morinda citrifolia sterilizing preparation and preparation method thereof - Google Patents
Morinda citrifolia sterilizing preparation and preparation method thereof Download PDFInfo
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Abstract
The invention provides a morinda citrifolia sterilizing preparation and a preparation method thereof, wherein the sterilizing preparation comprises the following raw materials in parts by weight: 12 to 30 parts of Adenophora trachelioides Trachelioides extract, 10 to 25 parts of stellera chamaejasme extract, 8 to 15 parts of horseradish extract, 3 to 5 parts of semen lepidii oil and 0.3 to 1.5 parts of shikimic acid, wherein the mixture ratio of the raw materials synergistically plays a role in bacteriostasis, the extract has high bactericidal activity, particularly has a higher control effect on pythium aphanidermatum, anthrax, fusarium and soot bacteria, and has a higher control effect on the stem rot and anthracnose of Morinda citrifolia caused by the fungus, the control effects after 14 days respectively reach 86.2 percent and 84.2 percent, and the control effects after 14 days of anthracnose are 85.7 percent and 84.5 percent.
Description
Technical Field
The invention relates to the field of agricultural sterilization, and particularly relates to a morinda citrifolia sterilization preparation and a preparation method thereof.
Background
Morinda citrifolia (scientific name: morinda citrifolia L.) is called Morinda citrifolia, noni, is a shrub or small arbor of the genus Morinda of the family Rubiaceae, up to 5 meters in height; the stem is straight and the branch is close to the quadrangular shape. The leaves are alternately opposite, the two ends are gradually sharp or sharp, the leaves are glossy and have no hair, the two sides of the veins are convex, and the axilla of the veins below are densely covered with short hair; leaves of the Tuoye should be kept between the stems of the leaves without hair. Head-shaped inflorescences are arranged one by one, are opposite to leaves, have most flowers and have no stems; the calyx vessels are bonded to each other, and the calyx eave is truncated; the corolla is white, funnel-shaped, split-piece egg-shaped and needle-shaped, the throat part of the corolla is planted with flowers with filaments about 3 mm, the anther is inward, the style is about as long as the crown canal, the ovary is sometimes sterile, 1 ovule in each chamber is slightly flat, the ovule gathers flowers, nuts and berries, and the flower is oval, small in seed, flat and long in shape, and the flower season all year round. The whole plant has medicinal value, can assist in treating various diseases, such as obesity, hypertension, diabetes, constipation, liver disease, cancer, apoplexy, myocardial infarction and the like, and can play a role in inhibiting nervous depression, relieving pressure, balancing blood pressure, improving immune system, promoting the health and relaxing the bowels and the like; noni leaves contain many physiochemical components, mainly including antioxidants and bioflavonoids, such as terpene compounds, sterols, fatty acids, glycosides, terpene glycosides, flavonoid glycosides, etc.
The morinda citrifolia is rich in nutrition and high in medicinal value, the morinda citrifolia is planted in China at an early stage, yunnan and the like in China, but the growth of the morinda citrifolia is threatened by the damage of plant diseases and insect pests in the planting and cultivating process, researches show that the morinda citrifolia leaves, fruits and tender stems are damaged by the anthrax, the initial stage of lesions on the damaged leaves is nearly round and light red, and the outer ring of the lesions is reddish brown; a plurality of black brown cushion-shaped conidium discs grow on the later stage lesion spots, and the centers of diseased tissues become dry and thin and are paper-shaped; finally, perforation is carried out, the disease spots are connected together to form larger disease spots in severe cases, after the fruits and the tender stems are damaged, the disease spots which are slightly sunken downwards, nearly round and tan are formed, and black brown cushion-shaped conidiophore discs grow out in the later period; in addition, the stem rot is caused by infection of pythium, anthracnose and fusarium, the separation frequency of pathogenic bacteria in different ecological areas is different, and the separation frequency of pathogenic bacteria in the same ecological area is obviously different from year to year and from area to area. In rainy regions, pythium type stalk rot is often the main cause, and in arid regions, fusarium type stalk rot is the main cause. Diseases caused by pathogenic bacteria threaten the growth of morinda citrifolia, so a high-efficiency bactericidal preparation suitable for bacteriostasis and disease prevention of morinda citrifolia planting needs to be researched.
Disclosure of Invention
In view of this, the present invention provides a morinda citrifolia bactericidal preparation and a preparation method thereof, which solve the above problems.
The technical scheme of the invention is realized as follows: a morinda citrifolia bactericidal preparation comprises: the feed comprises the following raw materials in parts by weight: 12 to 30 parts of adenophora trachelioides maxim extract, 10 to 25 parts of stellera chamaejasme extract, 8 to 15 parts of horseradish extract, 3 to 5 parts of lepidium seed oil and 0.3 to 1.5 parts of shikimic acid.
Further, the morinda citrifolia bactericidal preparation comprises the following components: the feed comprises the following raw materials in parts by weight: adenophora trachelioides extract 16 parts, stellera chamaejasme extract 18 parts, horseradish extract 11 parts, lepidium seed oil 4 parts, and shikimic acid 0.9 part.
Further, the extract of adenophorea trachelioides is prepared by removing impurities from fresh adenophorea trachelioides rhizomes, grinding, sieving with a 60-100 mesh sieve, extracting three times in a solvent consisting of water, ethanol, methanol and acetic acid according to a mass ratio of 100.
Further, the stellera chamaejasme extract is prepared by adding 2-4 times by mass of distilled water into dry roots of stellera chamaejasme, soaking for 30-60 min, taking out, boiling with big fire (1700-2100W), decocting with small fire (700-1200W) for 40-60 min, filtering, repeatedly extracting with water for 2 times, combining filtrates obtained in three times, concentrating to a concentration of 80-120 mg/mL, and storing at 3-5 ℃ to obtain the stellera chamaejasme extract.
Further, the horseradish extract is a horseradish whole plant extract, fresh horseradish whole plants are taken and crushed, sieved by 80-120 meshes, soaked in ethyl acetate or ethanol with the mass of 4-6 times that of the powder for dry powder, kept stand for 20-30 h, ultrasonically extracted for 1-3 h, subjected to vacuum filtration and continuous extraction for three times, combined with filtrate, concentrated to 50-80 mg/mL, and dried to obtain the horseradish whole extract.
Further, the preparation method of the morinda citrifolia bactericidal preparation comprises the following steps:
s1, adding a stellera chamaejasme extract into deionized water, heating to 40-60 ℃, and stirring for 10-30 min to obtain a mixed material A;
s2, stirring and heating the extract of the Adenophora rimotiflora and the extract of the horseradish extract to 70-90 ℃ at constant speed, and preserving heat for 40-60 min to obtain a mixed material B;
and S3, mixing the mixed material A and the mixed material B, dropwise adding shikimic acid, and homogenizing at a high speed at the temperature of 60-65 ℃ to obtain the morinda citrifolia bactericidal preparation.
Further, the mass volume ratio of the S1 stellera chamaejasme extract to deionized water is 5-10.
Further, the S3 high-speed homogenizing rotation speed is 5000-7000 rpm, and the homogenizing lasts for 18-25 min.
Compared with the prior art, the invention has the beneficial effects that:
the sterilization preparation has high bactericidal activity, particularly has the bactericidal activity against pythium, anthracnose, fusarium and soot bacteria which are more than 90 percent, has high control effect on the morinda officinalis stalk rot and the anthracnose caused by the fungus, and has the control effects of 86.2 percent and 84.2 percent after 14 days and 85.7 percent and 84.5 percent after 14 days; the sterilization preparation has high sterilization activity on the morinda citrifolia plant and is not easy to generate resistance; the adenophora trachelioides extract, the stellera chamaejasme extract and the horseradish extract are extracted through a specific extraction process, so that antibacterial active ingredients of the adenophora trachelioides extract, the stellera chamaejasme extract and the horseradish extract are fully extracted, the shikimic acid can increase the antibacterial performance of pathogenic bacteria aiming at the infection of the morinda officinalis pathogenic bacteria effectively, and the lepidium seed oil can promote the mixing of the adenophora trachelioides extract and the adenophora trachelioides extract in the preparation process, so that the antibacterial performance is improved.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
A Morinda citrifolia bactericidal preparation: the feed comprises the following raw materials in parts by weight: adenophora trachelioides extract 12 parts, stellera chamaejasme extract 10 parts, horseradish extract 8 parts, lepidium seed oil 3 parts, and shikimic acid 0.3 parts;
extracting Adenophora trachelioides extract at 60 deg.C for three times to obtain supernatant, and filtering to obtain Adenophora trachelioides extract, wherein the Adenophora trachelioides extract is prepared by removing impurities from fresh Adenophora trachelioides rhizome, pulverizing, sieving with 60 mesh sieve, adding into a solvent comprising water, ethanol, methanol and acetic acid at a mass ratio of 100;
the stellera chamaejasme extract is prepared by soaking dried root of stellera chamaejasme in distilled water 2 times the weight of the dried root for 30min, taking out, boiling with strong fire, decocting with slow fire for 40min, filtering, repeatedly extracting with water for 2 times, mixing filtrates, concentrating to 80mg/mL, and storing at 3 deg.C to obtain stellera chamaejasme extract;
the horseradish extract is a horseradish whole plant extract, fresh horseradish whole plants are crushed and sieved by a 80-mesh sieve, ethyl acetate with the volume 4 times that of the horseradish whole plants is used for soaking dry powder, the mixture is kept stand for 20 hours, ultrasonic extraction is carried out for 1 hour, vacuum filtration and continuous extraction are carried out for three times, filter liquor is combined, the mixture is concentrated to 50mg/mL, and the horseradish whole extract is obtained after drying.
Example 2
A Morinda citrifolia bactericidal preparation: the feed comprises the following raw materials in parts by weight: adenophora trachelioides extract 30 parts, stellera chamaejasme extract 25 parts, horseradish extract 15 parts, lepidium seed oil 5 parts, and shikimic acid 1.5 parts;
extracting Adenophora trachelioides extract at 90 deg.C for three times to obtain supernatant, and filtering to obtain Adenophora trachelioides extract, wherein the Adenophora trachelioides extract is prepared by removing impurities from fresh Adenophora trachelioides rhizomes, pulverizing, sieving with 100 mesh sieve, adding into a solvent comprising water, ethanol, methanol and acetic acid at a mass ratio of 100;
the stellera chamaejasme extract is prepared by adding dried roots of stellera chamaejasme into distilled water with the mass 4 times of that of the dried roots of stellera chamaejasme, soaking for 60min, taking out, boiling with strong fire, decocting with slow fire for 60min, filtering, repeatedly extracting with water for 2 times, combining three filtrates, concentrating to the concentration of 120mg/mL, and storing at 5 ℃ to obtain the stellera chamaejasme extract;
the horseradish extract is a horseradish whole plant extract, fresh horseradish whole plants are crushed and sieved by a 120-mesh sieve, dry powder is soaked in ethanol with the mass 6 times that of the powder, the mixture is kept stand for 30 hours, ultrasonic extraction is carried out for 3 hours, vacuum filtration and continuous extraction are carried out for three times, filtrate is combined, the mixture is concentrated to 80mg/mL, and the horseradish whole extract is obtained after drying.
Example 3
A morinda citrifolia bactericidal preparation comprises: the feed comprises the following raw materials in parts by weight: adenophora trachelioides extract 16 parts, stellera chamaejasme extract 18 parts, horseradish extract 11 parts, lepidium seed oil 4 parts, and shikimic acid 0.9 part;
extracting Adenophora trachelioides extract at 70 deg.C for three times to obtain supernatant, and filtering to obtain Adenophora trachelioides extract, wherein the Adenophora trachelioides extract is prepared by removing impurities from fresh Adenophora trachelioides rhizomes, pulverizing, sieving with 80 mesh sieve, adding into a solvent comprising water, ethanol, methanol and acetic acid at a mass ratio of 100;
the stellera chamaejasme extract is prepared by soaking dried root of stellera chamaejasme in 3 times of distilled water for 50min, taking out, boiling with strong fire, decocting with slow fire for 50min, filtering, repeatedly extracting with water for 2 times, mixing the three filtrates, concentrating to concentration of 100mg/mL, and storing at 4 deg.C to obtain stellera chamaejasme extract;
the horseradish extract is a horseradish whole plant extract, fresh horseradish whole plants are crushed and sieved by a 100-mesh sieve, ethyl acetate with the mass 5 times that of the powder is used for soaking dry powder, standing is carried out for 25 hours, ultrasonic extraction is carried out for 2 hours, vacuum filtration and continuous extraction are carried out for three times, filtrates are combined, concentrated to 60mg/mL, and the horseradish whole extract is obtained after drying.
The above examples 1 to 3 were prepared by the following method:
s1, adding stellera chamaejasme extract into deionized water, wherein the mass volume ratio of the stellera chamaejasme extract to the deionized water is (g/mL) 8;
s2, heating the extract of the horseradish and the extract of the Adenophora rimotiflora to 80 ℃ under uniform stirring, adding the lepidium seed oil, mixing, and keeping the temperature for 50min to obtain a mixed material B;
and S3, mixing the mixture A and the mixture B, dropwise adding shikimic acid, and homogenizing at a high speed of 6000rpm at 63 ℃ for 22min to obtain the morinda citrifolia bactericidal preparation.
Example 4
The difference between the embodiment and the embodiment 3 is that the extract from adenophorea trachelioides is obtained by removing impurities from fresh adenophorea trachelioides rhizomes, pulverizing, sieving with a 80-mesh sieve, extracting three times at 70 ℃ in a solvent composed of water and ethanol with a mass ratio of 100.
Example 5
The difference between the embodiment and the embodiment 3 is that the stellera chamaejasme extract is obtained by adding 3 times of methanol into the dried root of stellera chamaejasme to soak for 50min, taking out, boiling with strong fire, then decocting for 50min with slow fire, filtering, repeating water extraction for 2 times, combining the three filtrates, concentrating to the concentration of 100mg/mL, and storing at 4 ℃.
Example 6
The difference between the embodiment and the embodiment 3 is that the mass-volume ratio of the S1 stellera chamaejasme extract to the deionized water is 2 g/mL, which is specifically the following preparation method:
s1, adding stellera chamaejasme extract into deionized water, wherein the mass-volume ratio of the stellera chamaejasme extract to the deionized water is (g/mL) 2;
s2, stirring and heating the extract of the radix adenophorae trachelioides and the extract of the adenophora trachelioides maxim at a constant speed to 80 ℃, adding the lepidium seed oil, mixing, and keeping the temperature for 50min to obtain a mixed material B;
and S3, mixing the mixture A and the mixture B, dropwise adding shikimic acid, and homogenizing at a high speed of 6000rpm at 63 ℃ for 22min to obtain the morinda citrifolia bactericidal preparation.
Comparative example 1
The difference between the comparative example and the example 3 is that the morinda citrifolia bactericidal preparation comprises the following raw materials in parts by weight: adenophora trachelioides extract 10 parts, radix Euphorbiae Fischerianae extract 30 parts, herba Solani Nigri extract 5 parts, semen Lepidii oil 8 parts, and shikimic acid 2 parts;
the preparation method of the morinda citrifolia sterilizing preparation comprises the following steps: the method comprises the following steps:
s1, adding stellera chamaejasme extract into deionized water, wherein the mass-volume ratio of the stellera chamaejasme extract to the deionized water is 8 g/mL;
s2, heating the extract of the horseradish and the extract of the Adenophora rimotiflora to 80 ℃ under uniform stirring, adding the lepidium seed oil, mixing, and keeping the temperature for 50min to obtain a mixed material B;
and S3, mixing the mixture A and the mixture B, dropwise adding shikimic acid, and homogenizing at a high speed of 6000rpm at 63 ℃ for 22min to obtain the morinda citrifolia bactericidal preparation.
Comparative example 2
The difference between the comparative example and the example 3 is that shikimic acid is not added into the raw materials of the morinda officinalis sterilizing preparation, and the morinda officinalis sterilizing preparation comprises the following raw materials in parts by weight: adenophora trachelioides extract 16 parts, stellera chamaejasme extract 18 parts, horseradish extract 11 parts, and lepidium seed oil 4 parts;
the preparation method of the morinda citrifolia sterilizing preparation comprises the following steps:
s1, adding stellera chamaejasme extract into deionized water, wherein the mass-volume ratio of the stellera chamaejasme extract to the deionized water is 8 g/mL;
s2, heating the extract of the horseradish and the extract of the Adenophora rimotiflora to 80 ℃ under uniform stirring, adding the lepidium seed oil, mixing, and keeping the temperature for 50min to obtain a mixed material B;
and S3, mixing the mixed material A and the mixed material B, and homogenizing at a high speed of 6000rpm at 63 ℃ for 22min to obtain the morinda citrifolia bactericidal preparation.
Comparative example 3
The difference between the comparative example and the example 3 is that the raw materials of the morinda citrifolia bactericidal preparation are not added with the pepperweed seed oil, and the morinda citrifolia bactericidal preparation comprises the following raw materials in parts by weight: adenophora trachelioides extract 16 parts, stellera chamaejasme extract 18 parts, horseradish extract 11 parts and shikimic acid 0.9 part;
the preparation method of the morinda citrifolia bactericidal preparation comprises the following steps:
s1, adding stellera chamaejasme extract into deionized water, wherein the mass-volume ratio of the stellera chamaejasme extract to the deionized water is 8 g/mL;
s2, stirring and heating the extract of the adenophora rimotiflora and the extract of the adenophora rimotiflora at a constant speed to 80 ℃, and preserving heat for 50min to obtain a mixed material B;
and S3, mixing the mixture A and the mixture B, dropwise adding shikimic acid, and homogenizing at a high speed of 6000rpm at 63 ℃ for 22min to obtain the morinda citrifolia bactericidal preparation.
1. In vitro antifungal Activity test
The bactericidal formulations prepared in the above examples 1 to 6 and comparative examples 1 to 3 were diluted 100 times for activity test to determine the inhibitory effects of pythium, anthracnose, fusarium and soot on morinda citrifolia plants; the test is carried out on a sterile operating platform, all strains are cultured on PDA at 26 ℃ for 5 days, and the mycelium is ensured to have stronger activity and is used for identifying the antifungal activity. A cake of about 5 mm in diameter was cut out from the medium full of mycelia, inoculated into a petri dish containing a bactericidal preparation using a sterile inoculating needle, and cultured at 26 ℃ for 5 days. Sterile distilled water was used as a negative control, three replicates for each treatment condition in the three examples. Fungal colony diameter length was measured. The inhibition rate was calculated using the following formula:
I(％)＝〔(C－T)/(C－0.5)〕×100
c: diameter of fungal growth on control PDA;
t: diameter of fungi on treated PDA;
i: the inhibition rate.
TABLE 1 bacteriostatic Activity of pathogenic bacteria
From the above table, the bactericidal preparation prepared by the invention has higher bacteriostatic activity on pythium, anthracnose, fusarium and soot; the highest embodiment 3 is used, and the comparison between embodiment 3 and comparative example 1 shows that the weight parts of the raw materials can synergistically exert the bacteriostatic effect, the addition of shikimic acid in comparative example 2 can enhance the bacteriostatic performance on pathogenic bacteria, and the comparison between lepidium seed oil and comparative example 3 can show that the lepidium seed oil can promote the mixing of the horseradish extract and the adenophora trachelioides extract, so that the bacteriostatic performance is improved.
2. Potted plant bactericidal activity test
The bactericidal preparation of example 3 was diluted and used to measure the inhibitory effect on the basal rot and anthracnose of morinda citrifolia. In the experiment, a spraying mode is adopted, the sterilization preparations with different concentrations are uniformly sprayed on the surface and the rhizome of the leaf of the morinda citrifolia, and the pathogenic bacteria are inoculated after 7 days and 14 days of treatment. The inoculated leaves are cultured under the conditions of 25-30 ℃ and relative humidity of 80-85%. Equal volume of distilled water was used as negative control and commercial drug as positive control, as detailed in table 2. The infection degree is calculated according to the disease grade of each pathogenic bacterium.
Basal rot of morinda citrifolia
The classification criteria are as follows:
no disease spot at level 0;
grade 1, the lesion area is less than 5 percent of the whole rhizome area;
grade 3, the lesion spots account for 6 to 10 percent of the whole rhizome area;
grade 5, the lesion area accounts for 11 to 20 percent of the whole rhizome area;
7, the lesion area accounts for 21 to 40 percent of the whole rhizome area;
the area of the 9-grade lesion spots accounts for more than 40 percent of the whole rhizome area.
The prevention and treatment effect calculation formula is as follows:
disease index = Σ (disease grade number × root number of disease grade)/total number of leaf and root examined × highest grade number × 100
Control effect (%) = (control disease index-treatment group disease index)/(control disease index) × 100
TABLE 2 EXAMPLE 3 prevention and treatment of Stem rot disease in Morinda citrifolia plants with the fungicidal formulations
The above is an average value, "-" indicates that there is no data
Morinda citrifolia anthracnose
The classification criteria were as follows:
no lesion spot is found at level 0;
grade 1, the lesion area is less than 5 percent of the whole leaf area;
grade 3, the lesion area accounts for 6 to 10 percent of the whole leaf area;
grade 5, the lesion spot area accounts for 11 to 20 percent of the whole leaf area;
7 grade, the lesion spot area accounts for 21 to 40 percent of the whole leaf area;
the area of the 9-grade lesion spots accounts for more than 40 percent of the whole leaf area.
The prevention and treatment effect calculation formula is as follows:
disease index = Σ (number of disease stages × number of diseased leaves at that stage)/total number of investigated leaves × number of highest stages × 100
Control effect (%) = (control disease index-treatment group disease index)/(control disease index) × 100
TABLE 3 EXAMPLE 3 controlling Effect of the fungicidal formulations on the anthracnose of Morinda citrifolia plants
The above is an average value, "-" indicates that there is no data
From the results, the sterilization preparation has good control effects on the stem basal rot and the anthracnose of the morinda citrifolia plants, the control effects on the stem basal rot after 14 days respectively reach 86.2% and 84.2%, the control effects on the anthracnose after 14 days respectively reach 85.7% and 84.5%, the sterilization effect is higher than that of a commercial bactericide, and the control effects are obvious.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. The morinda citrifolia bactericidal preparation is characterized in that: the feed comprises the following raw materials in parts by weight: adenophora trachelioides extract 12 to 30 parts, stellera chamaejasme extract 10 to 25 parts, horseradish extract 8 to 15 parts, lepidium seed oil 3 to 5 parts and shikimic acid 0.3 to 1.5 parts;
extracting Adenophora trachelioides extract from fresh Adenophora trachelioides rhizomes at 60-100 mesh after removing impurities, placing the extract in a solvent consisting of water, ethanol, methanol and acetic acid with the mass ratio of 100;
the stellera chamaejasme extract is prepared by soaking dried roots of stellera chamaejasme in distilled water of 2 to 4 times of the mass for 30 to 60min, taking out, boiling with strong fire, decocting with slow fire for 40 to 60min, filtering, repeatedly extracting with water for 2 times, combining filtrates obtained in three times, concentrating until the concentration is 80 to 120mg/mL, and preserving at 3 to 5 ℃ to obtain the stellera chamaejasme extract;
the horseradish extract is a whole horseradish extract, fresh horseradish is crushed, sieved through 80-120 meshes, soaked in ethyl acetate or ethanol with the mass being 4-6 times of that of the powder, kept stand for 20-30h, extracted through ultrasonic for 1-3h, subjected to vacuum filtration, extracted for three times continuously, filtrates are combined, concentrated to 50-80mg/mL, and dried to obtain the horseradish extract.
2. The morinda citrifolia bactericidal formulation of claim 1, wherein: the feed comprises the following raw materials in parts by weight: adenophora trachelioides extract 16 parts, stellera chamaejasme extract 18 parts, horseradish extract 11 parts, lepidium seed oil 4 parts, and shikimic acid 0.9 part.
3. The method for preparing a sterilizing formulation of Morinda citrifolia according to claim 1, wherein the steps of: the method comprises the following steps:
s1, adding the stellera chamaejasme extract into deionized water, heating to 40-60 ℃, and stirring for 10-30min to obtain a mixed material A;
s2, heating the extract of the Adenophora rimotiflora and the extract of the Adenophora rimotiflora to 70 to 90 ℃ under uniform stirring, adding the Adenophora rimotiflora seed oil, mixing, and keeping the temperature for 40 to 60min to obtain a mixed material B;
and S3, mixing the mixture A and the mixture B, dropwise adding shikimic acid, and homogenizing at a high speed under the conditions of 60-65 ℃ to obtain the morinda citrifolia bactericidal preparation.
4. The method for preparing the Morinda citrifolia formulation according to claim 3, wherein: the mass-volume ratio of the S1 stellera chamaejasme extract to the deionized water is 5-10 in g/mL.
5. The method for preparing the Morinda citrifolia formulation according to claim 3, wherein the steps of: and the high-speed homogenizing rotation speed of the S3 is 5000-7000 rpm, and the homogenizing lasts for 18-25min.
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