CN113462395A - Method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer - Google Patents

Method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer Download PDF

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CN113462395A
CN113462395A CN202110723315.9A CN202110723315A CN113462395A CN 113462395 A CN113462395 A CN 113462395A CN 202110723315 A CN202110723315 A CN 202110723315A CN 113462395 A CN113462395 A CN 113462395A
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soil
microbial inoculum
bacillus
mixed microbial
fertilizer
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韩博
安娜
樊严
许锦
刘思彤
滕达
刘鹏
李铁新
李亚军
张东娟
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Jinzhou Academy Of Science And Technology
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Jinzhou Academy Of Science And Technology
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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/14Soil-conditioning materials or soil-stabilising materials containing organic compounds only

Abstract

The invention belongs to the field of farmland soil improvement, and particularly relates to a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer. Putting the corn straws into a crusher for crushing, then carrying out thermal cracking on the corn straws, and preparing the corn straws into biochar after natural cooling; uniformly applying the mixed microbial inoculum into farmland soil according to the proportion of 200 kg/mu; when the base fertilizer is applied in farmland cultivation, the application amount of the fertilizer is 30-50% of the conventional application amount, the normal sorghum fertilization amount per mu is 30 kg, and the compound fertilizer is applied by 9-15 kg per mu. The method is simple and easy to implement, needs to supplement the mixed microbial inoculum once every year, relieves the hardening and acidification of farmland soil, improves the farmland soil fertility, reduces the soil volume weight, promotes the accumulation of organic matters in the farmland soil, is beneficial to the growth of farmland soil microbial floras, improves the rhizosphere environment of crops, reduces the using amount of farmland fertilizers, reduces the occurrence of crop diseases and insect pests, and improves the utilization rate of the fertilizers.

Description

Method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer
Technical Field
The invention relates to the technical field of agricultural waste recycling and farmland soil improvement in agricultural resource environment protection, in particular to a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer.
Background
Soil acidification refers to a leaching process of alkali ions such as calcium, magnesium and the like in soil after being replaced by exchangeable aluminum hydroxide ions, and mainly shows that soil particles are dispersed, and water-stable granular structures of the soil are damaged; microorganisms in the soil are reduced, the mineralization environment condition of nutrient components in the soil is changed, particularly, the mineralization release proportion of various elements in the soil is increased, soil hardening is finally caused, and the yield and the quality of crops are rapidly reduced.
The soil hardening refers to that the soil has poor permeability and low oxygen content, and various cations are reduced and adsorbed on the root surfaces of crops to form a layer of nutrition barrier, so that the crops are difficult to absorb nutrition, and the crops grow slowly. The anaerobic microorganisms propagate in a large amount in an anoxic environment, and the generated toxins and harmful gases cause the root systems of crops to be difficult to grow, aggravate diseases and finally cause the death of the root systems.
The soil conditioner has the effects of regulating the physical and chemical properties of soil, loosening the structure, improving the water holding capacity of the soil, improving the soil fertility, improving the fertilizer holding capacity, improving the microbial community structure of the soil, fixing and repairing heavy metals and the like, and is widely applied to the aspect of soil improvement.
The biochar is a product of thermal cracking of biomass energy raw materials like common charcoal, and the main component of the biochar is carbon molecules. As a new soil conditioner, the biomass charcoal has important application value in the aspects of relieving soil acidification, improving soil quality, improving crop growth environment and the like due to the special physicochemical property of the biomass charcoal. The method for preparing the biochar by using the corn straws is also an effective method for treating waste gas in agricultural production. The method for producing the biochar by using the corn straws has the double carbon reduction effect, meets the requirements of agricultural clean production and resource circulation, and effectively protects the agricultural ecological environment.
The microbial fertilizer can greatly reduce the using amount of the fertilizer, reduce soil pollution and enhance the disease resistance and stress resistance of crops.
The treatment of soil hardening aims to improve the living environment of soil microorganisms. Firstly, the microorganisms should be ventilated by appropriate deep ploughing; secondly, the physical and chemical properties of the soil can be adjusted by measures such as reasonable fertilization, reasonable irrigation, reasonable pesticide spraying, acid adjustment, salt washing and the like; sufficient organic materials are repeatedly supplemented, the food requirement of the microorganisms is met, and the activity of the microorganisms is improved.
Due to the continuous planting for a long time, the number of harmful microorganisms in the soil is increasing. The excessive harmful microorganisms occupy the living space of the beneficial microorganisms, and the beneficial microorganisms are gradually reduced, so that the roots of the crops are easy to be damaged, the growth of the crops is limited, and even the crops die suddenly. Research shows that after soil is hardened, organic matters and biological bacterial manure are applied, which is beneficial to improving the granular structure of soil, improving the air permeability of soil and increasing the number of beneficial microorganisms.
Disclosure of Invention
The invention provides a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer, aiming at solving the technical problems in the prior art, aiming at improving the soil, reducing the input amount of the chemical fertilizer and improving the utilization rate of the chemical fertilizer, and having the advantages of simple process, strong operability, low cost and obvious effect, realizing the recycling and reutilization of agricultural production resources and reducing the agricultural non-point source pollution.
In order to achieve the purpose, the invention provides the following technical scheme: a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer comprises the following steps:
the method comprises the following steps: crushing the corn straws in a crusher to a length within 5cm, thermally cracking the corn straws at the temperature of 450-550 ℃ in an oxygen-free condition for 2-5 hours, and naturally cooling to prepare biochar;
step two: uniformly applying the mixed microbial inoculum into soil 30 days before farmland cultivation at the ratio of 100-200 kg/mu, and fully mixing the soil of a plough layer with the mixed microbial inoculum;
step three: when the base fertilizer is applied in farmland cultivation, the application amount of the fertilizer is 30-50% of the conventional application amount, the normal sorghum fertilization amount per mu is 30 kg, and the compound fertilizer is applied by 9-15 kg per mu.
Preferably, in the second step, the mixed microbial inoculum consists of the following components: 1000-2000 parts of biochar and 10-20 parts of composite microbial agent.
Preferably, the compound microbial agent consists of bacillus subtilis, bacillus licheniformis, bacillus laterosporus, bacillus megaterium, bacillus amyloliquefaciens and trichoderma harzianum.
Preferably, the strain content of the bacillus subtilis is not less than 500 hundred million/g, the strain content of the bacillus licheniformis is not less than 500 hundred million/g, the strain content of the bacillus laterosporus is not less than 100 hundred million/g, the strain content of the bacillus megaterium is not less than 100 hundred million/g, the strain content of the bacillus amyloliquefaciens is not less than 500 hundred million/g, and the strain content of the trichoderma aspergillum is not less than 10 hundred million/g.
Preferably, the preparation method of the bacillus subtilis and bacillus amyloliquefaciens microbial inoculum comprises the following steps:
(1) activating strains: respectively inoculating the bacillus subtilis and bacillus amyloliquefaciens strains which are stored at low temperature on a plate culture medium, and culturing for 24-26 hours at the temperature of 28-39 ℃; then selecting a single colony to inoculate on a slant culture medium, culturing for 24-26 hours at 30-39 ℃, washing the surface of the culture medium by using sterile water, and taking the eluent as inoculation liquid;
(2) preparing a seed solution: inoculating the inoculation liquid prepared in the step (1) into a liquid culture medium according to the proportion volume percentage of 1-10%, and performing shake culture at 26-36 ℃ for 12-18 hours to obtain a seed liquid;
(3) fermentation culture: and (3) inoculating the seed liquid obtained in the step (2) into a corn meal and soybean cake powder culture medium according to the proportion volume percentage of 3-10%, and culturing again to respectively obtain liquid preparations of bacteria A and bacteria B.
Preferably, in the step (3), the culture is performed for 22-28 hours at a temperature of 26-36 ℃ and a shaker rotation speed of 160-220 rpm/min.
Preferably, the corn meal and soybean cake powder culture medium comprises the following components in percentage by weight: 2.5 to 3.5 percent of cane sugar, 1.5 to 2.5 percent of corn flour, 1.5 to 2.5 percent of bean cake powder, 0.1 to 0.2 percent of NaCl, CaCO3 0.2%-0.3%,KH2PO40.01% -0.03% and MgSO4·7H200.02-0.04% and the balance of water.
Compared with the prior art, the invention has the beneficial effects that:
(1) the method is simple and easy to implement, and the mixed preparation needs to be supplemented every year, so that the soil environment for crop growth is improved, the heavy metal pollution of the soil is relieved, the farmland soil environment is improved, the application amount of farmland fertilizers is effectively reduced, and the utilization rate of the fertilizers is improved;
(2) the invention relates to a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer, which relieves the soil hardening and acidification of farmland, improves the soil fertility of farmland, reduces the soil volume weight, promotes the accumulation of organic matters in the farmland soil, is beneficial to the growth of microbial floras in the farmland soil, improves the rhizosphere environment of crops, reduces the using amount of fertilizer in the farmland and reduces the occurrence of crop diseases and insect pests by the combined action of a mixed preparation and the chemical fertilizer;
(3) the invention relates to a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer, which can greatly reduce the using amount of the chemical fertilizer, reduce soil pollution and enhance the disease resistance and stress resistance of crops by using a microbial fertilizer, is beneficial to improving the granular structure of the soil, improving the air permeability of the soil and increasing the number of beneficial microorganisms by applying organic matters and the biological microbial fertilizer.
Drawings
FIG. 1 is a graph of the analysis of sorghum photosynthetic data according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 and tables 1 to 5, and the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme of a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer, which comprises the following steps:
a method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer comprises the following steps:
the method comprises the following steps: crushing the corn straws in a crusher to a length within 5cm, thermally cracking the corn straws at the temperature of 450-550 ℃ in an oxygen-free condition for 2-5 hours, and naturally cooling to prepare biochar;
step two: uniformly applying the mixed microbial inoculum into soil 30 days before farmland cultivation at the ratio of 100-200 kg/mu, and fully mixing the soil of a plough layer with the mixed microbial inoculum, wherein the mixed microbial inoculum consists of the following components: 1000-2000 parts by weight of biochar and 10-20 parts by weight of composite microbial agent, wherein the composite microbial agent comprises bacillus subtilis, bacillus licheniformis, bacillus laterosporus, bacillus megaterium, bacillus amyloliquefaciens and trichoderma harzianum, the strain content of the bacillus subtilis is not lower than 500 hundred million/g, the strain content of the bacillus licheniformis is not lower than 500 hundred million/g, the strain content of the bacillus laterosporus is not lower than 100 hundred million/g, the strain content of the bacillus megaterium is not lower than 100 hundred million/g, the strain content of the bacillus amyloliquefaciens is not lower than 500 hundred million/g, and the strain content of the trichoderma harzianum is not lower than 10 hundred million/g;
step three: when the base fertilizer is applied in farmland cultivation, the application amount of the fertilizer is 30-50% of the conventional application amount, the normal sorghum fertilization amount per mu is 30 kg, and the compound fertilizer is applied by 9-15 kg per mu.
The preparation method of the bacillus subtilis and bacillus amyloliquefaciens microbial inoculum comprises the following steps of:
(1) activating strains: respectively inoculating the bacillus subtilis and bacillus amyloliquefaciens strains which are stored at low temperature on a plate culture medium, and culturing for 24-26 hours at the temperature of 28-39 ℃; then selecting a single colony to inoculate on a slant culture medium, culturing for 24-26 hours at 30-39 ℃, washing the surface of the culture medium by using sterile water, and taking the eluent as inoculation liquid;
(2) preparing a seed solution: inoculating the inoculation liquid prepared in the step (1) into a liquid culture medium according to the proportion volume percentage of 1-10%, and performing shake culture at 26-36 ℃ for 12-18 hours to obtain a seed liquid;
(3) fermentation culture: inoculating the seed liquid obtained in the step (2) into a corn meal and bean cake powder culture medium according to the proportion volume percentage of 3-10%, and culturing for 22-28 hours under the conditions that the temperature is 26-36 ℃ and the rotating speed of a shaking table is 160-220rpm/min, so as to respectively obtain liquid preparations of bacteria A and bacteria B, wherein the corn meal and bean cake powder culture medium comprises the following components in percentage by weight: 2.5 to 3.5 percent of cane sugar, 1.5 to 2.5 percent of corn flour, 1.5 to 2.5 percent of bean cake powder, 0.1 to 0.2 percent of NaCl, CaCO30.2%-0.3%,KH2PO40.01% -0.03% and MgSO4·7H200.02-0.04% and the balance of water.
Example 1
A method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer comprises the following steps:
the method comprises the following steps: putting corn straws into a crusher to be crushed, crushing the corn straws to the length within 5cm, thermally cracking the corn straws at 500 ℃ under the anaerobic condition for 3 hours, and naturally cooling to prepare biochar;
step two: uniformly applying the mixed microbial inoculum into soil 30 days before farmland cultivation in a proportion of 200 kg/mu, and fully mixing the soil of a plough layer with the mixed microbial inoculum, wherein the mixed microbial inoculum consists of the following components: 1500 parts by weight of biochar and 10 parts by weight of a composite microbial agent, wherein the composite microbial agent consists of bacillus subtilis, bacillus licheniformis, bacillus laterosporus, bacillus megaterium, bacillus amyloliquefaciens and trichoderma harzianum, the strain content of the bacillus subtilis is not lower than 500 hundred million/gram, the strain content of the bacillus licheniformis is not lower than 500 hundred million/gram, the strain content of the bacillus laterosporus is not lower than 100 hundred million/gram, the strain content of the bacillus megaterium is not lower than 100 hundred million/gram, the strain content of the bacillus amyloliquefaciens is not lower than 500 hundred million/gram, and the strain content of the trichoderma harzianum is not lower than 10 hundred million/gram;
step three: when the base fertilizer is applied in farmland cultivation, the application amount of the fertilizer is 50 percent of the conventional application amount, the normal sorghum fertilization amount per mu is 30 kilograms, and the compound fertilizer is applied per mu by 15 kilograms. The sorghum cultivation method is the same as the conventional method.
Example 2
A method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer comprises the following steps:
the method comprises the following steps: putting corn straws into a crusher to be crushed, crushing the corn straws to the length within 5cm, thermally cracking the corn straws at 500 ℃ under the anaerobic condition for 3 hours, and naturally cooling to prepare biochar;
step two: uniformly applying the mixed microbial inoculum into soil 30 days before farmland cultivation in a proportion of 200 kg/mu, and fully mixing the soil of a plough layer with the mixed microbial inoculum, wherein the mixed microbial inoculum consists of the following components: 1500 parts by weight of biochar and 10 parts by weight of a composite microbial agent, wherein the composite microbial agent consists of bacillus subtilis, bacillus licheniformis, bacillus laterosporus, bacillus megaterium, bacillus amyloliquefaciens and trichoderma harzianum, the strain content of the bacillus subtilis is not lower than 500 hundred million/gram, the strain content of the bacillus licheniformis is not lower than 500 hundred million/gram, the strain content of the bacillus laterosporus is not lower than 100 hundred million/gram, the strain content of the bacillus megaterium is not lower than 100 hundred million/gram, the strain content of the bacillus amyloliquefaciens is not lower than 500 hundred million/gram, and the strain content of the trichoderma harzianum is not lower than 10 hundred million/gram;
step three: when the base fertilizer is applied in farmland cultivation, the application amount of the fertilizer is 30 percent of the conventional application amount, the normal sorghum fertilization amount per mu is 30 kg, and the compound fertilizer is applied 9 kg per mu. The sorghum cultivation method is the same as the conventional method.
Example 3
A method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer comprises the following steps:
the method comprises the following steps: putting the corn straws into a crusher for crushing, crushing the corn straws to the length within 5cm, then carrying out thermal cracking on the corn straws at 550 ℃ under the anaerobic condition for 5 hours, and preparing the biochar after natural cooling;
step two: uniformly applying the mixed microbial inoculum into soil 30 days before farmland cultivation in a proportion of 150 kg/mu, and fully mixing the soil of a plough layer with the mixed microbial inoculum, wherein the mixed microbial inoculum consists of the following components: 2000 parts by weight of biochar and 20 parts by weight of composite microbial inoculant, wherein the composite microbial inoculant consists of bacillus subtilis, bacillus licheniformis, bacillus laterosporus, bacillus megaterium, bacillus amyloliquefaciens and trichoderma harzianum, the strain content of the bacillus subtilis is not lower than 500 hundred million/gram, the strain content of the bacillus licheniformis is not lower than 500 hundred million/gram, the strain content of the bacillus laterosporus is not lower than 100 hundred million/gram, the strain content of the bacillus megaterium is not lower than 100 hundred million/gram, the strain content of the bacillus amyloliquefaciens is not lower than 500 hundred million/gram, and the strain content of the trichoderma harzianum is not lower than 10 hundred million/gram;
step three: when the base fertilizer is applied in farmland cultivation, the application amount of the fertilizer is 50 percent of the conventional application amount, the normal sorghum fertilization amount per mu is 30 kilograms, and the compound fertilizer is applied per mu by 15 kilograms. The sorghum cultivation method is the same as the conventional method.
Benefit case:
the sorghum variety is cultivated by using the brocade 109, and 25 sorghum plants are planted at the row spacing of 55cm and the plant spacing of 20cm and the density of 6000 sorghum plants per mu, wherein each ridge is 5 m. 8 ridges are a cell, 22m2200 plants.
Case 1: a pot experiment is adopted, soil for planting sorghum is selected and added with the mixed preparation of the embodiment 1 and the embodiment 2 and the chemical fertilizer combined action modifier to improve the soil, and the sorghum is planted. And (4) testing the volume weight of the soil and the content of heavy metal in the soil. The experiment designed five treatments. Processing one: the modifier (CK) is not applied to the fertilizer cultivation; and (5) processing: modifier (T1) of inventive example 1 was added; and (3) treatment III: modifier (T2) of inventive example 2 was added; and (4) treatment: modifier (T3) of inventive example 3 was added; and (5) processing: the soil was not cultivated with any crop, and the soil was improved by adding the improver of the present invention example 1, S0 before the addition of the improver, and S1 after the addition of the improver. All the soil is test area soil for cultivating sorghum all year round before planting. A randomized block experimental design was used with 3 replicates per treatment setup.
The test results are as follows:
1. comparison of soil fertility index, heavy metal content and volume weight before and after soil improvement
TABLE 1 comparison of soil fertility index, heavy metal content and volume weight before and after soil improvement
As can be seen from Table 1, the soil fertility, the heavy metal content of the soil and the volume weight of the soil after the conditioner is added are obviously improved, that is, the theoretical properties of the soil are greatly improved by adding the soil conditioner into the soil, and the theoretical properties are supported.
2. Effect of adding soil conditioner on sorghum vegetative growth
TABLE 2 comparative analysis of plant height, stem thickness, leaf area and chlorophyll content of different treatments of sorghum
As can be seen from table 2: in the aspect of plant height, each treatment is higher than CK, the highest T3 is, and the number of T2 is; in the aspect of stem thickness, T1 and T2 are lower than CK, and T3 is slightly higher than CK; in terms of leaf area, T1 and T2 are lower than CK, and T3 is higher than CK; in terms of chlorophyll content, each treatment was higher than CK, with the highest T3 and the highest T2 times.
From FIG. 1, it can be seen that T1, T3 are on par with CK in terms of net photosynthetic rate, stomatal conductance and transpiration rate, and T2 is higher than the other treatments; in terms of intercellular CO2 concentration, T1 was on par with CK, T2 and T3 were higher than CK, and T3 was the highest.
3. Effect of adding soil conditioner on sorghum reproductive growth
TABLE 4 sorghum process yield comparative analysis Table
Treatment of Yield (Kg)
CK 49.69
T1 51.50
T2 65.48
T3 54.95
As is clear from Table 4, the yields of each treatment were higher than CK, with the highest T2 yield and the highest T3 times.
TABLE 5 comparative analysis table of sorghum treatment quality
As can be seen from Table 5, the sorghum quality was mostly higher than CK for each treatment, with the highest T3 content of total starch, amylose, followed by T2; the highest T2, less T3 times, in terms of tannin content.
The application amount of the fertilizer is 50% of the conventional application amount, 2000 parts by weight of biochar is matched and mixed with 20 parts by weight of compound microbial agent (T3), and the fertilizer is suitable for the vegetative growth of sorghum; the application amount of the fertilizer is 30% of the conventional application amount, 1500 parts by weight of biochar is matched and mixed with 10 parts by weight of compound microbial agent (T2), and the compound microbial agent is suitable for reproductive growth of sorghum. In conclusion, the cost factor is considered, the application amount of the fertilizer is 30% of the conventional application amount, and the biochar with 1500 parts by weight is mixed with 10 parts by weight of the compound microbial agent, so that the compound microbial agent is more suitable for the growth of sorghum.
The proportion formula of the invention not only can greatly improve and repair soil, but also reduces cost and reduces pressure for farmers.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. A method for improving soil by combined action of a mixed microbial inoculum and a chemical fertilizer is characterized by comprising the following steps:
the method comprises the following steps: crushing the corn straws in a crusher to a length within 5cm, thermally cracking the corn straws at the temperature of 450-550 ℃ in an oxygen-free condition for 2-5 hours, and naturally cooling to prepare biochar;
step two: uniformly applying the mixed microbial inoculum into soil 30 days before farmland cultivation at the ratio of 100-200 kg/mu, and fully mixing the soil of a plough layer with the mixed microbial inoculum;
step three: when the base fertilizer is applied in farmland cultivation, the application amount of the fertilizer is 30-50% of the conventional application amount, the normal sorghum fertilization amount per mu is 30 kg, and the compound fertilizer is applied by 9-15 kg per mu.
2. The method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer as claimed in claim 1, which is characterized in that: in the second step, the mixed microbial inoculum consists of the following components: 1000-2000 parts of biochar and 10-20 parts of composite microbial agent.
3. The method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer as claimed in claim 2, which is characterized in that: the compound microbial agent consists of bacillus subtilis, bacillus licheniformis, bacillus laterosporus, bacillus megaterium, bacillus amyloliquefaciens and trichoderma harzianum.
4. The method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer as claimed in claim 3, wherein: the strain content of the bacillus subtilis is not lower than 500 hundred million/g, the strain content of the bacillus licheniformis is not lower than 500 hundred million/g, the strain content of the bacillus laterosporus is not lower than 100 hundred million/g, the strain content of the bacillus megaterium is not lower than 100 hundred million/g, the strain content of the bacillus amyloliquefaciens is not lower than 500 hundred million/g, and the strain content of the trichoderma is not lower than 10 hundred million/g.
5. The method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer as claimed in claim 3, wherein: the preparation method of the bacillus subtilis and bacillus amyloliquefaciens microbial inoculum comprises the following steps:
(1) activating strains: respectively inoculating the bacillus subtilis and bacillus amyloliquefaciens strains which are stored at low temperature on a plate culture medium, and culturing for 24-26 hours at the temperature of 28-39 ℃; then selecting a single colony to inoculate on a slant culture medium, culturing for 24-26 hours at 30-39 ℃, washing the surface of the culture medium by using sterile water, and taking the eluent as inoculation liquid;
(2) preparing a seed solution: inoculating the inoculation liquid prepared in the step (1) into a liquid culture medium according to the proportion volume percentage of 1-10%, and performing shake culture at 26-36 ℃ for 12-18 hours to obtain a seed liquid;
(3) fermentation culture: and (3) inoculating the seed liquid obtained in the step (2) into a corn meal and soybean cake powder culture medium according to the proportion volume percentage of 3-10%, and culturing again to respectively obtain liquid preparations of bacteria A and bacteria B.
6. The method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer as claimed in claim 5, wherein: in the step (3), the culture is carried out for 22-28 hours under the conditions that the temperature is 26-36 ℃ and the rotating speed of the shaking table is 160-220 rpm/min.
7. The method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer as claimed in claim 5, wherein: the corn meal and bean cake powder culture medium comprises the following components in percentage by weight: 2.5 to 3.5 percent of cane sugar, 1.5 to 2.5 percent of corn flour, 1.5 to 2.5 percent of bean cake powder, 0.1 to 0.2 percent of NaCl, CaCO3 0.2%-0.3%,KH2PO40.01% -0.03% and MgSO4·7H200.02-0.04% and the balance of water.
CN202110723315.9A 2021-06-28 2021-06-28 Method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer Pending CN113462395A (en)

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CN105462595A (en) * 2015-12-21 2016-04-06 武汉飞喜乐农业科技有限公司 Acid soil modifier and preparation method thereof
CN110305671A (en) * 2019-06-25 2019-10-08 江苏里下河地区农业科学研究所 One kind is for facilities vegetable continuous cropping soil biological modification agent and its preparation and application method
CN110606782A (en) * 2019-09-30 2019-12-24 广东森度生态农业科技有限公司 Farmland soil water-retaining organic fertilizer and preparation method thereof

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