CN113455426A - Breeding method of low-salt-resistant large yellow croaker - Google Patents
Breeding method of low-salt-resistant large yellow croaker Download PDFInfo
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- 241001596950 Larimichthys crocea Species 0.000 title claims abstract description 71
- 238000009395 breeding Methods 0.000 title claims abstract description 40
- 235000013601 eggs Nutrition 0.000 claims abstract description 59
- 230000012447 hatching Effects 0.000 claims abstract description 37
- 241000251468 Actinopterygii Species 0.000 claims abstract description 25
- 238000012258 culturing Methods 0.000 claims abstract description 20
- 238000011534 incubation Methods 0.000 claims abstract description 19
- 230000001418 larval effect Effects 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 210000002149 gonad Anatomy 0.000 claims abstract description 4
- 230000009027 insemination Effects 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 230000001488 breeding effect Effects 0.000 claims description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 238000005273 aeration Methods 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 5
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 241000234295 Musa Species 0.000 description 2
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 2
- MCSXGCZMEPXKIW-UHFFFAOYSA-N 3-hydroxy-4-[(4-methyl-2-nitrophenyl)diazenyl]-N-(3-nitrophenyl)naphthalene-2-carboxamide Chemical compound Cc1ccc(N=Nc2c(O)c(cc3ccccc23)C(=O)Nc2cccc(c2)[N+]([O-])=O)c(c1)[N+]([O-])=O MCSXGCZMEPXKIW-UHFFFAOYSA-N 0.000 description 1
- 241000251464 Coelacanthiformes Species 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241001596954 Larimichthys Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
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- 230000001617 migratory effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a breeding method of low-salt-resistant large yellow croaker, which adopts a double-layer culture pond comprising an upper hatching pond and a lower culture pond and comprises the following steps: performing large yellow croaker insemination operation in water with salinity of 20-30 per mill, sequentially cleaning and sterilizing obtained fertilized eggs, and transferring to a hatching pond; controlling the salinity of the hatching pond to be 11 per thousand, hatching the fertilized eggs of the large yellow croaker, and then introducing the hatched larval fish into a culture pond; controlling the initial salinity of the culture pond to be 11 per mill, then reducing the salinity of the culture pond to 4-6 per mill at the speed of reducing the salinity by 1 per day, and continuously culturing the large yellow croakers under the salinity condition until the physique mass is more than 1 kg; introducing large yellow croakers with the weight of more than 1kg into an incubation pool for gonad enhancement cultivation to be used as low-salt-resistant parents; and performing induced spawning, collection of fertilized eggs and disinfection treatment in an incubation pool at salinity of 20-30 per mill until the initial larval fish is cultivated; and (4) introducing the opening fries into a culture pond again, and performing low-salt cultivation of multi-generation offspring in a mode that the salinity gradient in the step (3) is reduced to 4-6 per mill.
Description
Technical Field
The invention belongs to the technical field of fish culture, and particularly relates to a breeding method of low-salt-resistant large yellow croakers.
Background
The large yellow croaker (Larimiches crocea) belongs to Osteichthyes, Perc-iformes, Scienidaceae and Larimichthys, is a warm water cluster migratory fish, is mainly distributed in south China sea, east China sea and south China sea, and is deeply loved by consumers due to delicious meat quality, golden yellow body color, deep red lips and lucky symbols. The yield of 2019 reaches 22.6 ten thousand tons, and the method is the first to breed the marine fishes in China, and becomes one of the largest-scale marine cage-bred fishes and eight dominant export-bred aquatic products in China. At present, large yellow croaker is mainly cultured on the sea, but the culture process is greatly influenced by areas, so that the economic value and market income of the large yellow croaker in different culture areas are greatly different, and in addition, the pest and disease infection harm of the large yellow croaker is also an important factor influencing the economic value and the market income. Research proves that the best method for treating most of marine fish culture pests is to reduce the salinity of the culture environment, because most of the pests cannot survive in a low-salt environment, and the low-salt pond and the low-salt estuary area in the inland area are wide, so that the area capable of culturing low-salt species is large. However, the existing low-salt-resistant fish breeding technology only has certain breakthrough on Chinese black pond carps and groupers, although the large yellow croakers are hot spots for research, few cases of success exist, and if the low-salt-resistant fish breeding technology can be developed and cultured and popularized in inland low-salt ponds and estuaries, considerable practical value and economic benefit can be brought.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a breeding method of low-salt-resistant large yellow croaker, which can breed a new large yellow croaker variety with salinity resistance of 4-6 per mill. The technical scheme of the invention is as follows:
a breeding method of low-salt-resistant large yellow croakers adopts a double-layer culture pond, wherein the double-layer culture pond comprises an incubation pond at the upper layer and a culture pond at the lower layer, and the breeding method comprises the following steps:
step 2, controlling the salinity of the hatching pond to be 11 per mill, hatching the fertilized eggs of the large yellow croaker, and then introducing the hatched larval fish into a breeding pond;
step 4, introducing the large yellow croakers with the weight of more than 1kg into a hatching pool for gonad enhancement cultivation and taking the large yellow croakers as low-salt-resistant parents; and performing induced spawning, collection of fertilized eggs and disinfection treatment in an incubation pool at the salinity of 20 per mill to 30 per mill until the larval fish is cultivated into an open-mouth larval fish;
and 5, introducing the opening fry into a culture pond again, and performing low-salt cultivation of multi-generation offspring in a mode that the salinity gradient of the opening fry in the step 3 is reduced to 4-6 per mill.
Further, the bottom of the hatching pond and the bottom of the culture pond are provided with aeration pipelines which are connected with an oxygen supply device arranged outside the culture pond.
Furthermore, a water outlet for feeding the larval fish into the culture pond is formed in the bottom of the hatching pond.
Preferably, the multi-generation filial generation low-salt breeding is 5-generation filial generation low-salt breeding.
Further, the low-salt breeding of the 5 generations of filial generation specifically comprises the following steps:
(1) after F1 generation low-salt resistant parents are obtained by cultivation, selecting large yellow croakers with the body mass of more than 1kg in the F1 generation as the F2 generation parents, and hatching second generation fertilized eggs obtained by induced spawning at the salinity of 20 per mill to 30 per mill in a hatching pond at the salinity of 11 per mill until open offspring are obtained, and then culturing the fertilized eggs in a breeding pond at the salinity of 10 per mill until the body mass of the fertilized eggs is more than 1 kg;
(2) taking large yellow croaker with the body mass of more than 1kg of F2 generation as a parent of F3 generation, and carrying out induced spawning at the salinity of 20-30 per mill to obtain third generation fertilized eggs, incubating and culturing the third generation fertilized eggs in an incubation pool at the salinity of 11 per mill until open offspring, and culturing in a culture pool at the salinity of 7 per mill until the body mass is more than 1 kg;
(3) taking large yellow croaker with the body mass of more than 1kg of the F3 generation as a parent of the F4 generation, carrying out induced spawning at the salinity of 20 per mill to 30 per mill to obtain fourth generation fertilized eggs, incubating and culturing the fourth generation fertilized eggs in an incubation pool at the salinity of 11 per mill until open offspring, and culturing the fourth generation fertilized eggs in a culture pool at the salinity of 4 per mill to 6 per mill until the body mass of the fourth generation fertilized eggs is more than 1 kg;
(4) large yellow croaker with body mass of more than 1kg of F4 generation is further used as parent of F5 generation, spawning is induced at salinity of 20-30 per mill to obtain fifth generation fertilized eggs, the fifth generation fertilized eggs are incubated in an incubation pool at salinity of 11 per mill to culture open offspring, and the fertilized eggs are further cultured in a culture pool at salinity of 4-6 per mill to reach body mass of more than 1 kg.
Compared with the prior art, the invention has the beneficial effects that: the method provided by the invention is adopted to breed large yellow croakers, so that a new stable low-salt-resistant large yellow croaker variety can be obtained, the first successful cultivation of the low-salt-resistant large yellow croakers is initiated, and a reference is provided for the research of other low-salt-resistant fishes.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural diagram of a double-layer culture pond adopted by the embodiment of the invention,
wherein, 1, an oxygen tube; 2. controlling an oxygen switch; 3. oxygen gas; 4. a water outlet of the fertilized egg hatching pond; 5. a fertilized egg hatching pond; 6. and (5) forming a pond.
Detailed Description
In the description of the present invention, it is to be noted that those whose specific conditions are not specified in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturers. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The present invention will now be described in further detail with reference to the following figures and specific examples, which are intended to be illustrative, but not limiting, of the invention.
Example 1
The embodiment provides a breeding method of low-salt-resistant large yellow croakers, which is implemented by adopting a double-layer culture pond in a large yellow croaker culture farm in a banana urban area of Ningde city, and putting 100kg of fertilized eggs, wherein the specification of the culture pond is as follows: 40m3The double-layer culture pond comprises an incubation pond at the upper layer and a culture pond at the lower layer, and each culture pond is 20m3And the bottom parts of the hatching pond and the culture pond are provided with aeration pipelines which are connected with an oxygen supply device arranged outside the culture pond. And a water outlet for feeding the larval fish into the culture pond is formed in the bottom of the hatching pond. The breeding and the cultivation of the offspring seeds refer to the standard SC/T2089 and 2018, the technical specification for breeding the large yellow croaker and the NY/T5061-2002, the technical specification for breeding the large yellow croaker as a pollution-free food respectively. The breeding method comprises the following steps:
step 2, controlling the salinity of the hatching pond to be 11 per mill, hatching the fertilized eggs of the large yellow croaker, and then introducing the hatched larval fish into a breeding pond;
step 4, introducing the large yellow croakers with the weight of more than 1kg into a hatching pool for gonad enhancement cultivation and taking the large yellow croakers as low-salt-resistant parents; and performing induced spawning, collection of fertilized eggs and disinfection treatment in an incubation pool at the salinity of 20 per mill to 30 per mill until the larval fish is cultivated into an open-mouth larval fish;
and 5, introducing the opening fry into a culture pond again, and performing low-salt culture on 5 generations of filial generation according to the mode that the salinity gradient in the step 3 is reduced to 4-6 per mill.
The breeding of the 5 generations of filial generation specifically comprises the following steps:
(1) after F1 generation low-salt resistant parents are obtained by cultivation, selecting large yellow croakers with the body mass of more than 1kg in the F1 generation as the F2 generation parents, and hatching second generation fertilized eggs obtained by induced spawning at the salinity of 20 per mill to 30 per mill in a hatching pond at the salinity of 11 per mill until open offspring are obtained, and then culturing the fertilized eggs in a breeding pond at the salinity of 10 per mill until the body mass of the fertilized eggs is more than 1 kg;
(2) taking large yellow croaker with the body mass of more than 1kg of F2 generation as a parent of F3 generation, and carrying out induced spawning at the salinity of 20-30 per mill to obtain third generation fertilized eggs, incubating and culturing the third generation fertilized eggs in an incubation pool at the salinity of 11 per mill until open offspring, and culturing in a culture pool at the salinity of 7 per mill until the body mass is more than 1 kg;
(3) taking large yellow croaker with the body mass of more than 1kg of the F3 generation as a parent of the F4 generation, carrying out induced spawning at the salinity of 20 per mill to 30 per mill to obtain fourth generation fertilized eggs, incubating and culturing the fourth generation fertilized eggs in an incubation pool at the salinity of 11 per mill until open offspring, and culturing the fourth generation fertilized eggs in a culture pool at the salinity of 4 per mill to 6 per mill until the body mass of the fourth generation fertilized eggs is more than 1 kg;
(4) large yellow croaker with body mass of more than 1kg of F4 generation is further used as parent of F5 generation, spawning is induced at salinity of 20-30 per mill to obtain fifth generation fertilized eggs, the fifth generation fertilized eggs are incubated in an incubation pool at salinity of 11 per mill to culture open offspring, and the fertilized eggs are further cultured in a culture pool at salinity of 4-6 per mill to reach body mass of more than 1 kg.
The survival rate and the cultivation period of the large yellow croaker cultivated in this example are shown in table 1. The quality index of large yellow croaker is shown in Table 2.
TABLE 1 survival and development cycle of the selectively bred progenies of example 1
| Item | F1 | F2 | F3 | F4 | F5 |
| Hatching rate of fertilized eggs% | 53.2-56.9 | 63.4-65.4 | 70.2-71.3 | 78.6-79.7 | 82.4-89.5 |
| Survival rate of culture/%) | 29.8-30.6 | 30.0-31.2 | 31.2-33.3 | 31.1-34.6 | 32.8-36.4 |
| Cultivation period/year | 1.8 | 1.6 | 1.6 | 1.5 | 1.5 |
TABLE 2 Pseudosciaena crocea quality index
| Item | F1 | F2 | F3 | F4 | F5 |
| Body weight mean (g) | 760.8 | 781.0 | 781.6 | 782.1 | 782.9 |
| Average body length (cm) | 34.7 | 35.1 | 35.2 | 35.2 | 35.4 |
| Appearance of the product | Golden Yellow | Golden Yellow | Golden Yellow | Golden Yellow | Golden Yellow |
Example 2
The embodiment provides a breeding method of low-salt-resistant large yellow croakers, which adopts a double-layer culture pond and a certain large yellow croaker in Ningdefu ancient cityFertilized eggs are put in the culture farm by 200kg, and the specification of the culture pond is as follows: 50m3The water body of (2). The double-layer culture pond comprises an incubation pond on the upper layer and a culture pond on the lower layer, each of which is 25m3And the bottom parts of the hatching pond and the culture pond are provided with aeration pipelines which are connected with an oxygen supply device arranged outside the culture pond. And a water outlet for feeding the larval fish into the culture pond is formed in the bottom of the hatching pond. The breeding and the cultivation of the offspring seeds refer to the standard SC/T2089-. The breeding method is the same as that in example 1.
The survival rate and the growing period of the large yellow croaker bred in this example are shown in table 3. The large yellow croaker quality index is shown in table 4.
TABLE 3 survival and growth cycle of the selectively bred progenies of example 2
| Item | F1 | F2 | F3 | F4 | F5 |
| Hatching rate of fertilized eggs% | 51.0-53.4 | 61.1-64.3 | 68.1-72.5 | 79.1-80.9 | 81.7-89.7 |
| Survival rate of culture/%) | 28.7-29.5 | 31.1-32.4 | 32.1-34.2 | 32.3-35.7 | 33.3-35.6 |
| Cultivation period/year | 1.8 | 1.6 | 1.6 | 1.5 | 1.5 |
TABLE 4 Pseudosciaena crocea quality index
| Item | F1 | F2 | F3 | F4 | F5 |
| Body weight mean (g) | 779.8 | 783.2 | 786.4 | 786.8 | 788.2 |
| Average body length (cm) | 35.2 | 35.5 | 35.7 | 35.7 | 35.9 |
| Appearance of the product | Golden Yellow | Golden Yellow | Golden Yellow | Golden Yellow | Golden Yellow |
Example 3
The embodiment provides a breeding method of low-salt-resistant large yellow croakers, which adopts a double-layer culture pond, 300kg of fertilized eggs are put in a certain large yellow croaker culture farm in a banana city area in Ningde city, and the specification of the culture pond is as follows: 60m3The water body of (2). The double-layer culture pond comprises an incubation pond at the upper layer and a culture pond at the lower layer, each of which is 30m3And the bottom parts of the hatching pond and the culture pond are provided with aeration pipelines which are connected with an oxygen supply device arranged outside the culture pond. And a water outlet for feeding the larval fish into the culture pond is formed in the bottom of the hatching pond. The breeding and the cultivation of the offspring seeds refer to the standard SC/T2089-. The breeding method is the same as that in example 1.
The survival rate and the growing period of the large yellow croaker bred in this example are shown in table 5. The large yellow croaker quality index is shown in Table 6.
TABLE 5 survival and growth cycle of the selectively bred progenies of example 3
| Item | F1 | F2 | F3 | F4 | F5 |
| Hatching rate of fertilized eggs% | 53.1-57.2 | 64.0-65.5 | 66.9-70.9 | 74.7-78.2 | 79.9-82.4 |
| Survival rate of culture/%) | 30.8-31.1 | 30.7-31.7 | 32.6-33.9 | 33.6-35.7 | 34.2-37.2 |
| Cultivation period/year | 1.8 | 1.6 | 1.6 | 1.5 | 1.5 |
TABLE 6 Pseudosciaena crocea quality index
| Item | F1 | F2 | F3 | F4 | F5 |
| Body weight mean (g) | 779.2 | 781.3 | 783.3 | 789.7 | 793.2 |
| Average body length (cm) | 35.1 | 35.2 | 35.3 | 35.5 | 35.6 |
| Appearance of the product | Golden Yellow | Golden Yellow | Golden Yellow | Golden Yellow | Golden Yellow |
Through research in 10 years, the applicant creates the first successful cultivation of low-salt-resistant large yellow croakers, and the method for breeding the large yellow croakers can obtain a stable and low-salt-resistant new large yellow croaker variety and provide reference for research on other low-salt-resistant fishes.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (5)
1. A breeding method of low-salt-resistant large yellow croaker is characterized by comprising the following steps: adopts a double-layer culture pond, the double-layer culture pond comprises an incubation pond positioned on the upper layer and a culture pond positioned on the lower layer, and the breeding method comprises the following steps:
step 1, performing large yellow croaker insemination operation in water with salinity of 20-30 per mill, and transferring obtained fertilized eggs into a hatching pond after cleaning and sterilizing the fertilized eggs in sequence;
step 2, controlling the salinity of the hatching pond to be 11 per mill, hatching the fertilized eggs of the large yellow croaker, and then introducing the hatched larval fish into a breeding pond;
step 3, controlling the initial salinity of the culture pond to be 11 per mill, then reducing the salinity of the culture pond to 4 per mill to 6 per mill at the speed of reducing the salinity by 1 per mill every day, and continuously culturing the large yellow croakers under the salinity condition until the physique quantity is more than 1 kg;
step 4, introducing the large yellow croakers with the weight of more than 1kg into a hatching pool for gonad enhancement cultivation and taking the large yellow croakers as low-salt-resistant parents; and performing induced spawning, collection of fertilized eggs and disinfection treatment in an incubation pool at the salinity of 20 per mill to 30 per mill until the larval fish is cultivated into an open-mouth larval fish;
and 5, introducing the opening fry into a culture pond again, and performing low-salt cultivation of multi-generation offspring in a mode that the salinity gradient of the opening fry in the step 3 is reduced to 4-6 per mill.
2. The breeding method of low-salt-resistant large yellow croaker according to claim 1, wherein: and aeration pipelines are arranged at the bottoms of the hatching pond and the culture pond and are connected with an oxygen supply device arranged outside the culture pond.
3. The breeding method of low-salt-resistant large yellow croaker according to claim 1, wherein: and a water outlet for feeding the larval fish into the culture pond is formed in the bottom of the hatching pond.
4. The breeding method of low-salt-resistant large yellow croaker according to claim 1, wherein: the multi-generation filial generation low-salt breeding is 5-generation filial generation low-salt breeding.
5. The breeding method of low-salt-resistant large yellow croaker according to claim 4, wherein: the low-salt breeding of the 5 generations of filial generation specifically comprises the following steps:
(1) after F1 generation low-salt resistant parents are obtained by cultivation, selecting large yellow croakers with the body mass of more than 1kg in the F1 generation as the F2 generation parents, and hatching second generation fertilized eggs obtained by induced spawning at the salinity of 20 per mill to 30 per mill in a hatching pond at the salinity of 11 per mill until open offspring are obtained, and then culturing the fertilized eggs in a breeding pond at the salinity of 10 per mill until the body mass of the fertilized eggs is more than 1 kg;
(2) taking large yellow croaker with the body mass of more than 1kg of F2 generation as a parent of F3 generation, and carrying out induced spawning at the salinity of 20-30 per mill to obtain third generation fertilized eggs, incubating and culturing the third generation fertilized eggs in an incubation pool at the salinity of 11 per mill until open offspring, and culturing in a culture pool at the salinity of 7 per mill until the body mass is more than 1 kg;
(3) taking large yellow croaker with the body mass of more than 1kg of the F3 generation as a parent of the F4 generation, carrying out induced spawning at the salinity of 20 per mill to 30 per mill to obtain fourth generation fertilized eggs, incubating and culturing the fourth generation fertilized eggs in an incubation pool at the salinity of 11 per mill until open offspring, and culturing the fourth generation fertilized eggs in a culture pool at the salinity of 4 per mill to 6 per mill until the body mass of the fourth generation fertilized eggs is more than 1 kg;
(4) large yellow croaker with body mass of more than 1kg of F4 generation is further used as parent of F5 generation, spawning is induced at salinity of 20-30 per mill to obtain fifth generation fertilized eggs, the fifth generation fertilized eggs are incubated in an incubation pool at salinity of 11 per mill to culture open offspring, and the fertilized eggs are further cultured in a culture pool at salinity of 4-6 per mill to reach body mass of more than 1 kg.
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