CN113454230A - 一种通过体细胞重编程制备诱导多能干细胞的方法 - Google Patents

一种通过体细胞重编程制备诱导多能干细胞的方法 Download PDF

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CN113454230A
CN113454230A CN202080006440.1A CN202080006440A CN113454230A CN 113454230 A CN113454230 A CN 113454230A CN 202080006440 A CN202080006440 A CN 202080006440A CN 113454230 A CN113454230 A CN 113454230A
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reprogramming
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oct4
somatic
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CN113454230B (zh
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范靖
任芳
王安欣
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Zhejiang Huode Bioengineering Co ltd
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Abstract

提供了一种通过体细胞重编程制备诱导多能干细胞的方法以及由此得到的诱导多能干细胞。该方法包括将因子Oct4和Nanog作为重编程诱导因子导入体细胞后进行重编程；然后，将部分或完全重编程的体细胞培养在含有特定化学诱导剂的培养基中以得到诱导多能干细胞，该化学诱导剂包括TGFβ受体抑制剂、环AMP激动剂和糖原合成酶激酶抑制剂。

Description

PCT国内申请，说明书已公开。

Claims (11)

  1. PCT国内申请，权利要求书已公开。
CN202080006440.1A 2019-08-01 2020-07-31 一种通过体细胞重编程制备诱导多能干细胞的方法 Active CN113454230B (zh)

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CN115772505A (zh) * 2023-02-13 2023-03-10 淇嘉科技(天津)有限公司 促进体细胞重编程为诱导多能干细胞的培养基及方法

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CN112301052A (zh) * 2019-08-01 2021-02-02 浙江霍德生物工程有限公司 一种通过体细胞重编程制备诱导多功能干细胞的方法
CN113462638B (zh) * 2021-06-30 2022-10-25 呈诺再生医学科技(珠海横琴新区)有限公司 一种高效无遗传修饰的iPSC诱导、产业化单克隆挑取平台及应用
CN114277190B (zh) * 2021-12-31 2024-07-23 安徽中盛溯源生物科技有限公司 一种hiPSC中外源基因残留检测用特异性DNA片段、引物、试剂盒和检测方法
CN115975949B (zh) * 2023-03-20 2023-09-01 天九再生医学(天津)科技有限公司 一种基于microRNA制备诱导性多能干细胞的方法

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