CN113396920A - Application of albendazole in preparation of pesticide bactericide for preventing and treating postharvest chloromycete of citrus - Google Patents
Application of albendazole in preparation of pesticide bactericide for preventing and treating postharvest chloromycete of citrus Download PDFInfo
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- CN113396920A CN113396920A CN202110495500.7A CN202110495500A CN113396920A CN 113396920 A CN113396920 A CN 113396920A CN 202110495500 A CN202110495500 A CN 202110495500A CN 113396920 A CN113396920 A CN 113396920A
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- albendazole
- citrus
- chloromycete
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- 229960002669 albendazole Drugs 0.000 title claims abstract description 37
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 36
- 241000207199 Citrus Species 0.000 title claims abstract description 35
- HXHWSAZORRCQMX-UHFFFAOYSA-N albendazole Chemical compound CCCSC1=CC=C2NC(NC(=O)OC)=NC2=C1 HXHWSAZORRCQMX-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 239000000575 pesticide Substances 0.000 title claims abstract description 13
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 9
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 9
- 238000002360 preparation method Methods 0.000 title claims description 12
- 241001507673 Penicillium digitatum Species 0.000 claims description 5
- 230000000855 fungicidal effect Effects 0.000 claims description 3
- 239000000417 fungicide Substances 0.000 claims description 3
- 244000052769 pathogen Species 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 claims description 2
- 239000005820 Prochloraz Substances 0.000 abstract description 8
- TVLSRXXIMLFWEO-UHFFFAOYSA-N prochloraz Chemical compound C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl TVLSRXXIMLFWEO-UHFFFAOYSA-N 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 abstract description 3
- 229940097572 chloromycetin Drugs 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 description 14
- 239000003814 drug Substances 0.000 description 12
- 238000012360 testing method Methods 0.000 description 9
- 241000233866 Fungi Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 8
- 235000013399 edible fruits Nutrition 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 208000031888 Mycoses Diseases 0.000 description 3
- -1 albendazole compound Chemical class 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- DOMXUEMWDBAQBQ-WEVVVXLNSA-N terbinafine Chemical compound C1=CC=C2C(CN(C\C=C\C#CC(C)(C)C)C)=CC=CC2=C1 DOMXUEMWDBAQBQ-WEVVVXLNSA-N 0.000 description 3
- 229960002722 terbinafine Drugs 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- NMIZONYLXCOHEF-UHFFFAOYSA-N 1h-imidazole-2-carboxamide Chemical compound NC(=O)C1=NC=CN1 NMIZONYLXCOHEF-UHFFFAOYSA-N 0.000 description 1
- 241000946391 Alternaria citri Species 0.000 description 1
- 241001465677 Ancylostomatoidea Species 0.000 description 1
- 241000242722 Cestoda Species 0.000 description 1
- 241000498255 Enterobius vermicularis Species 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 241000190144 Lasiodiplodia theobromae Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000122123 Penicillium italicum Species 0.000 description 1
- 241001480007 Phomopsis Species 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- 241000209051 Saccharum Species 0.000 description 1
- 241000244177 Strongyloides stercoralis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 206010014881 enterobiasis Diseases 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 208000024386 fungal infectious disease Diseases 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
- A01N43/52—1,3-Diazoles; Hydrogenated 1,3-diazoles condensed with carbocyclic rings, e.g. benzimidazoles
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a new application of albendazole as a pesticide bactericide in preventing and treating citrus postharvest chloromycetin for the first time, and the albendazole is used for preventing and treating crop diseases caused by the citrus postharvest chloromycetin and has safety obviously higher than that of a commercial bactericide prochloraz.
Description
The technical field is as follows:
the invention relates to application of albendazole in preparation of a pesticide bactericide for preventing and treating citrus postharvest chloromycete.
Background art:
citrus belongs to the genus Citrus of the family Rutaceae, the planting area and the yield of the citrus are in the top, the citrus is spread over more than 100 countries, and the major production countries are China, Brazil, America and the like. Since 2008, our country has for the first time surpassed brazil and became the first major citrus producing country in the world. However, the national level of citrus production, namely the yield per unit area, the cost per fruit, the commercial fruit rate and the national gap between the scale of fruit growers and the development of citrus production, are still very large. The citrus in China is mainly sold fresh, the maturation period is basically concentrated in 9-11 months, the production area and the consumption area of the citrus have a certain distance, the maturation period is relatively concentrated, a certain storage and transportation period is needed to adjust the contradiction of market supply and demand, and during the period, fruit loss caused by microbial infection occurs greatly, such as rotting, nutrition and water loss frequently occurs, and great economic loss is caused. Among them, fungal diseases are most serious, and common fungal diseases include green mold (Penicillium digitatum), Penicillium disease (p. italicum), acid rot (oosporitrialeurotii), black stalk rot (Diplodia natalensis), brown stalk rot (Phomopsis cytoplasma), black rot (Alternaria citri), anthracnose (colletotrichoides), brown rot (phohthora citrophthora), and the like. In recent years, it has been found that 30% to 50% of postharvest rot of citrus in our country is caused by green mold (p. Therefore, the storage and preservation of citrus is the direction and hot spot of domestic and foreign development and research in the coming years.
Although breeding resistant varieties is the most economical and environmentally friendly method for controlling the diseases, the use of chemical agents in the case of lack of resistant varieties or incomplete resistance is still the main means for controlling fungal diseases of crops. Albendazole is used medically to expel roundworms, pinworms, tapeworms, whipworms, hookworms, strongyloides stercoralis, etc. However, at present, no related research on the resistance of albendazole to the postharvest pseudomonas aeruginosa disease of citrus is available.
The invention content is as follows:
the invention aims to provide application of albendazole in preparation of a pesticide bactericide for preventing and treating citrus postharvest chloromycete.
The invention is realized by the following technical scheme:
the application of albendazole shown as a formula I in preparing pesticide bactericide for preventing and treating citrus postharvest chloromycete is as follows:
the albendazole is used at a concentration of 0.05-10. mu.g/mL.
The pathogenic bacteria of the postharvest chlorophycea citrulli is Penicillium digitatum.
The invention has the following beneficial effects: the invention discovers the new application of albendazole serving as a pesticide bactericide in preventing and controlling citrus postharvest chloromycete for the first time. The pesticide composition can be used for preventing and treating crop diseases caused by the pathogenic bacteria Penicillium digitatum of the postharvest chlorophycea citri after citrus harvest, and the safety is obviously higher than that of the commercial fungicide prochloraz.
Description of the drawings:
FIG. 1 shows the inhibition of post-harvest Pythium species in citrus (day six).
FIG. 2 shows the inhibition of P.aeruginosa in citrus (4 days) at different concentrations of albendazole.
FIG. 3 is a graph showing the effect of different concentrations of albendazole on the occurrence of green mold in Saccharum sinensis Roxb.
The specific implementation mode is as follows:
the following is a further description of the invention and is not intended to be limiting.
Example 1: inhibition effect of albendazole on citrus postharvest chloromycetin
1) Test drug
Albendazole, prochloraz (positive control compound, commercial pesticide), terbinafine.
(2) Test fungal species
The number of the tested fungal strains is 1, and the fungal strains are Penicillium digitatum which is a pathogen of the citrus greening mycosis.
(3) Determination of anti-plant-fungal Activity
Preparation of test bacterium plates: under aseptic condition, respectively inoculating pure test bacteria to a sterile PDA culture medium plate by a dense wave line drawing method, culturing at 27 deg.C for about one week, and allowing the plate to grow bacterial lawn.
Preparation of the culture medium and sterilization thereof: the culture medium is PDA solid culture medium, and the formula is as follows: 200g of potato, 20g of glucose, 20g of agar and 1000mL of tap water. The preparation process comprises the following steps: cutting peeled potato into 5mm square pieces, adding tap water, boiling for 20min, filtering with 8 layers of gauze, adding corresponding amount of glucose and agar powder into the filtrate, heating and stirring to dissolve, adding tap water to desired volume, packaging into 250mL triangular bottles, sealing (each bottle contains 100mL culture medium), and sterilizing with autoclave at 115 deg.C for 30 min.
Preparing a liquid medicine: weighing 1.00mg of each of the compounds of prochloraz, albendazole and terbinafine in a 1.5mL sterile centrifuge tube, and respectively adding 1mL of DMSO for dissolving.
Preparation of a culture medium with medicine: heating and melting a culture medium in a sterilized triangular flask, cooling to about 50 ℃, respectively adding the prepared liquid medicine on a clean bench through aseptic operation, slightly shaking the triangular flask to uniformly distribute the liquid medicine in the culture medium, preparing albendazole into a culture medium with medicine with a final concentration of 10 mu g/mL, immediately pouring the medium into 5 sterile culture dishes (20 mL of culture medium in each culture dish) while the medium is hot to prepare flat plates with uniform thickness, and marking, wherein prochloraz is a positive control; in the same procedure, plates of negative control DMSO (0.1mL directly in 100mL sterile medium) were prepared and labeled.
And (3) testing the bacteriostatic rate: under the aseptic condition, punching a certain amount of fungus cakes on a test fungus plate by using an aseptic puncher, taking the fungus cakes by using an inoculating ring holder, placing the fungus cakes on a prepared culture medium with medicine, enabling the hyphae to face downwards, marking 1 fungus cake per dish (three times), placing the fungus cakes in a thermostat at 27 ℃ for culturing for 72 hours, taking out a culture, measuring the diameter of a bacterial colony by using a caliper (measuring the cross quantity twice, measuring all three repeated plates, taking the average number of the plates), calculating the bacteriostasis rate according to the formula (1), and obtaining the calculation result of the plant fungus activity resistance of the albendazole under the concentration of 10 mu g/mL in the table 1.
Inhibition rate (negative control growth diameter-treated growth diameter)/(negative control growth diameter-5) × 100% … (1).
As can be seen from the table 1, the albendazole compound has a good sterilization effect on citrus postharvest chloromycete, wherein the concentration of the albendazole compound is 10 mug/mL and can reach 100%.
TABLE 1 inhibition of plant fungi by albendazole and prochloraz compounds
| Compound (I) | Bacteriostatic ratio (%) |
| Prochloraz | 100 |
| Albendazole | 100 |
| Terbinafine | 43.91 |
Example 2: inhibition effect of albendazole with different concentrations on citrus postharvest chloromycete
Reference example 1, except that the preparation of the medium with drug: heating and melting a culture medium in a sterilized triangular flask, cooling to about 50 ℃, respectively adding the prepared liquid medicines on a clean bench through aseptic operation, slightly shaking the triangular flask to uniformly distribute the liquid medicines in the culture medium, preparing albendazole into medicine-carrying culture mediums with final concentrations of 0.05 mu g/mL, 0.075 mu g/mL, 0.10 mu g/mL, 0.125 mu g/mL, 0.15 mu g/mL and 0.175 mu g/mL respectively, immediately pouring the medicine-carrying culture mediums into 5 sterile culture dishes (20 mL of culture medium in each culture dish) to prepare a flat plate with uniform thickness when the medicine-carrying culture mediums are hot, and marking; in the same procedure, plates of negative control DMSO (0.1mL directly in 100mL sterile medium) were prepared and labeled.
The results of the calculation of the inhibitory activity of albendazole on citrus postharvest chloromycete at different concentrations are shown in table 2 and fig. 2.
TABLE 2 inhibitory Activity of albendazole at different concentrations against Citrus postharvest Chloromyces (4 days)
The virulence equation is: 5.1312x +9.9751, coefficient of correlation R2＝0.9595，EC50＝0.1072μg/ml，EC90＝0.1906μg/ml。
Example 3: inhibition effect of albendazole with different concentrations on citrus postharvest chloromycete
(1) Test drug
Albendazole, prochloraz (positive control compound, commercial pesticide).
(2) Test material
Sugar orange
(3) Inhibition effect of albendazole on pseudomonas aeruginosa of sugar orange after harvest
Preparing a liquid medicine: the compound albendazole, 100.00mg, was weighed into a 150mL sterile centrifuge tube and dissolved by adding 1mL of acetic acid, respectively.
And (3) testing the bacteriostatic rate: randomly dividing purchased sugar oranges into 12 groups after damaged fruits are removed, wherein each group contains more than or equal to 120 fruits. Soaking with medicinal preparation, treating every four groups with three treatments, wherein the final concentrations of the treated medicinal preparation are 0ug/ml, 0.4ug/ml and 40ug/ml respectively. And (5) airing, and counting the rotting rate after seven days. The results are shown in FIG. 3.
As is clear from Table 2 and FIG. 3, albendazole according to the present invention exhibits excellent inhibitory effect on Pseudomonas aeruginosa after Citrus fruit harvest, and EC thereof is clear from Table 250The average value is 0.1072 mug/mL; as can be seen from fig. 3, the albendazole at a low concentration significantly reduced the occurrence of green mold after harvest of sugar oranges. In conclusion, the albendazole provided by the invention has obvious inhibitory activity on the citrus postharvest pseudomonas aeruginosa, and the safety is obviously higher than that of the imidazole amide.
Claims (3)
1. The application of albendazole shown in formula 1 in preparing pesticide bactericide for preventing and treating citrus postharvest chloromycete is as follows:
2. the use of albendazole according to claim 1 for preparing a pesticide fungicide for controlling citrus postharvest chloromycete, wherein the albendazole concentration is 0.05-10 μ g/mL during use.
3. Use of albendazole according to claim 1 or 2 for the preparation of a pesticide fungicide for the control of citrus postharvest chloromycete, wherein the citrus postharvest chlorophycea pathogen is Penicillium digitatum.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110495500.7A CN113396920A (en) | 2021-05-07 | 2021-05-07 | Application of albendazole in preparation of pesticide bactericide for preventing and treating postharvest chloromycete of citrus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110495500.7A CN113396920A (en) | 2021-05-07 | 2021-05-07 | Application of albendazole in preparation of pesticide bactericide for preventing and treating postharvest chloromycete of citrus |
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| CN113396920A true CN113396920A (en) | 2021-09-17 |
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| CN202110495500.7A Pending CN113396920A (en) | 2021-05-07 | 2021-05-07 | Application of albendazole in preparation of pesticide bactericide for preventing and treating postharvest chloromycete of citrus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116508779A (en) * | 2023-04-17 | 2023-08-01 | 浙江大学 | Bactericide for preventing and treating bacterial fruit blotch of melons and application of bactericide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101990915A (en) * | 2010-10-21 | 2011-03-30 | 贵州道元生物技术有限公司 | Pesticide/bactericide for tree daubing |
| CN109744249A (en) * | 2017-11-01 | 2019-05-14 | 江苏莱科化学有限公司 | A kind of bactericidal composition |
| CN110554132A (en) * | 2019-10-11 | 2019-12-10 | 湖南农业大学 | Method for detecting residual amount of prothioconazole in citrus |
-
2021
- 2021-05-07 CN CN202110495500.7A patent/CN113396920A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990915A (en) * | 2010-10-21 | 2011-03-30 | 贵州道元生物技术有限公司 | Pesticide/bactericide for tree daubing |
| CN109744249A (en) * | 2017-11-01 | 2019-05-14 | 江苏莱科化学有限公司 | A kind of bactericidal composition |
| CN110554132A (en) * | 2019-10-11 | 2019-12-10 | 湖南农业大学 | Method for detecting residual amount of prothioconazole in citrus |
Non-Patent Citations (3)
| Title |
|---|
| LEVER, C. A.: "《Evaluation of Penicillium roqueforti growth in milk from albendazole-treated cows》", 《UNIV. IBEROAM., MEX.》 * |
| 石娅琼: "《丙硫唑抗菌谱及其增效减量应用技术研究》", 《南京农业大学硕士学位论文》 * |
| 邓光宙等: "几种农药对金柑黑点型柑橘树脂病的田间药效评价", 《南方园艺》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116508779A (en) * | 2023-04-17 | 2023-08-01 | 浙江大学 | Bactericide for preventing and treating bacterial fruit blotch of melons and application of bactericide |
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Application publication date: 20210917 |