CN113367246B - Feed for improving digestive tract function of poultry and preparation method thereof - Google Patents
Feed for improving digestive tract function of poultry and preparation method thereof Download PDFInfo
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- CN113367246B CN113367246B CN202110008323.5A CN202110008323A CN113367246B CN 113367246 B CN113367246 B CN 113367246B CN 202110008323 A CN202110008323 A CN 202110008323A CN 113367246 B CN113367246 B CN 113367246B
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- 244000144977 poultry Species 0.000 title claims abstract description 51
- 210000001035 gastrointestinal tract Anatomy 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims abstract description 51
- 244000025254 Cannabis sativa Species 0.000 claims abstract description 49
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000002699 waste material Substances 0.000 claims abstract description 39
- 239000000843 powder Substances 0.000 claims abstract description 34
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 24
- 238000003756 stirring Methods 0.000 claims abstract description 24
- 239000002131 composite material Substances 0.000 claims abstract description 23
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 17
- 239000001110 calcium chloride Substances 0.000 claims abstract description 17
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 17
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
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- 235000019779 Rapeseed Meal Nutrition 0.000 claims description 14
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- 241000235527 Rhizopus Species 0.000 claims description 12
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- 238000000034 method Methods 0.000 claims description 12
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- 239000002028 Biomass Substances 0.000 description 4
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- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
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- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
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- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a preparation method of a feed for improving the digestive tract function of poultry, which comprises the following steps: crushing and sieving the waste grass roots, ball-milling the waste grass roots by vacuum liquid nitrogen until the waste grass roots pass through a 100-mesh sieve, and obtaining pretreated grass roots, wherein the vacuum degree is 30-50 kpa; regulating the temperature of pretreated grass root to 30-40deg.C, and adding 1-5×10 active bacteria 9 Fermenting cfu/g of composite bacteria for 2-5h, adding silage bamboo shoot shell, soybean meal, rice bran, peanut cake and calcium chloride water solution, and mixingUniformly carrying out hydrothermal reaction for 20-40min at 105-115 ℃, cooling to room temperature, and grinding to obtain superfine crushed grass roots; mixing superfine pulverized radix Glehniae, semen Maydis, testa Tritici, vegetable cake, semen Tritici Aestivi, shell powder and radix Puerariae powder, homogenizing under 30-50MPa to obtain premix; adding taurine, grape seed procyanidine, calcium bicarbonate and salt into the premix, stirring uniformly, granulating and obtaining the feed for improving the digestive tract function of poultry.
Description
Technical Field
The invention relates to the technical field of poultry feeds, in particular to a feed for improving the digestive tract function of poultry and a preparation method thereof.
Background
The key problems in the development of the farming industry are the health level and productivity of the animals. How to accelerate the growth of poultry and reduce the disease rate is a long-term concern in the field of poultry farming. With the rapid development of modern poultry farming industry in China, the problems of shortage of conventional feed resources, rising of feed raw material prices and the like are increasingly remarkable, and the development of non-grain feed is an important way for relieving the shortage of feed resources, reducing the raising cost and improving the economic benefit.
The lignocellulose biomass is the renewable biomass resource which is most abundant in the earth, and the lignocellulose biomass is used for preparing the feed, so that the high-value utilization of the waste biomass can be realized, and the problems of high price and dependence on import of the feed can be solved. The main components in lignocellulose are crude fibers such as cellulose, hemicellulose, lignin and the like; the food entering the digestive tract is decomposed into glucose and amino acid under the action of various digestive juice, so that after the food is digested and absorbed, the residue is discharged out of the body, but the digestive tract of the poultry is short and has small volume, so that the feed passes through the digestive tract at a higher speed, and the digestive absorption of nutrient substances is incomplete, so that the digestive tract diseases with different degrees are easily induced for a long time.
At present, in the intensive cultivation process of modern poultry, a method of adding feed antibiotics is adopted to prevent diseases, but the use of the antibiotics causes a plurality of problems, such as drug residues, drug resistance, body immunity reduction and the like, and the application of the antibiotics is greatly limited.
Disclosure of Invention
Based on the technical problems in the background technology, the invention provides a feed for improving the digestive tract function of poultry and a preparation method thereof.
A preparation method of feed for improving digestive tract function of poultry comprises the following steps:
s1, crushing and sieving waste grass roots, ball-milling the waste grass roots by vacuum liquid nitrogen until the waste grass roots pass through a 100-mesh sieve, wherein the vacuum degree is 30-50kpa, and the mass-volume ratio g/L of the waste grass roots to the liquid nitrogen is 10:1-4, obtaining pretreated grass roots;
s2, regulating the temperature of the pretreated grass roots to 30-40 ℃, and adding 1-5 multiplied by 10 of the total number of effective living bacteria 9 Fermenting cfu/g of composite bacteria for 2-5h, adding silage bamboo shoot shell, soybean meal, rice bran, peanut cake and calcium chloride aqueous solution, uniformly mixing, performing hydrothermal reaction at 105-115 ℃ for 20-40min, cooling to room temperature, and grinding to obtain superfine crushed grass roots;
s3, uniformly mixing superfine crushed grass roots, corns, bran, rapeseed meal, wheat, shell powder and kudzuvine root powder, and homogenizing at 30-50MPa to obtain a premix;
s4, adding taurine, grape seed procyanidine, calcium bicarbonate and salt into the premix, uniformly stirring, and granulating to obtain the feed for improving the digestive tract function of poultry.
Preferably, in S2, the complex bacteria include: geotrichum candidum, microzyme, rhizopus, bacillus subtilis and trichoderma viride.
Preferably, in S2, the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 10:1-4:1-4:1-2: 1-2.
Preferably, in the S2, the concentration of the calcium chloride aqueous solution is 0.1-0.5mol/L, and the mass ratio of the pretreated alkali grass roots, the compound bacteria, the silage bamboo shoot shells, the soybean meal, the rice bran, the peanut cakes and the calcium chloride aqueous solution is 100:1-2:30-40:4-12:4-10:10-20:200-500.
Preferably, in S2, grinding is performed with a colloid mill, running for 20-40min with a gap of 20-30 μm, and running for 5-15min with a gap of 10-15 μm.
Preferably, in S3, the mass ratio of the superfine crushed grass roots, corns, bran, rapeseed meal, wheat, shell powder and kudzuvine root powder is 100:20-40:1-5:2-8:5-15:1-5:1-2.
Preferably, in S3, a homogenizer is used for homogenizing treatment, and the inlet temperature of the homogenizer is 10-20 ℃.
Preferably, in S4, the mass ratio of premix, taurine, grape seed procyanidine, calcium bicarbonate and salt is 100:1-2:0.1-0.6:1-2:0.1-1.
The feed for improving the digestive tract function of the poultry is prepared by adopting the feed preparation method for improving the digestive tract function of the poultry.
The technical effects of the invention are as follows:
alkali grass is one of important pastures on natural grasslands, has strong penetration and encroachment capability of the roots and stems of the alkali grass, can form strong root nets, has extremely high content of root system crude fibers, and is extremely difficult to digest and is generally discarded.
According to the invention, waste alkali grass roots are sprayed with liquid nitrogen under the vacuum condition of a ball mill cavity, the hard structure of the waste alkali grass roots can be quickly embrittled, composite bacteria are added for fermentation after ball milling, lignocellulose can be degraded and easily absorbed components such as soluble sugar are obtained, digestion and absorption of food are effectively promoted, the pH value of a system can be effectively regulated by lactic acid and acetic acid generated in the fermentation process, growth and reproduction of pathogenic bacteria can be inhibited, gastrointestinal damage caused by pathogenic bacteria and toxins is reduced, gastrointestinal mucosa repair is promoted, maillard reaction can be effectively inhibited in a high-pressure reaction kettle under an acidic environment, degradation of plant cell walls is promoted by matching with calcium chloride, the content of nutrient substances, especially the extraction degree of fat, protein and hemicellulose content is extremely high, in addition, hemicellulose can be converted into oligosaccharides, anti-nutritional factor phytic acid is converted into inositol, phosphoric acid, anti-nutritional factor acid-soluble lignin is extremely easily absorbed by intestinal tracts, and colloid effect is matched to destroy microbial and plant cell final anti-degradation barrier, thus the regulation and repair of poultry digestive tract can be realized, and the poultry is promoted, and the meat can be fully utilized in a high-growth ratio.
The invention adopts the fermented grass roots and the silage bamboo shoot shells as main materials, the crude protein content of the feed can reach more than 25 percent, the total sum of 16 amino acids is more than 8.15g/100g, the protein content of the feed is comprehensively improved, the cellulose content is reduced, and the feed is compounded with other raw materials, so that the digestive tract regulation and repair of poultry can be realized, the number of harmful bacteria in the digestive tract of the poultry is reduced, the development of the digestive tract of the poultry is promoted, the absorption of nutrient substances is improved, the immunity and slaughtering performance of the poultry are improved, and the muscle quality is improved.
Drawings
FIG. 1 is an anatomic diagram of the myostomach and the adeno-stomach after feeding AA broiler chickens with the poultry feed obtained in example 5;
FIG. 2 is an anatomic view of myostomach and adenostomach after a control group AA broiler test;
FIG. 3 is a graph showing the myogastric tissue sections of a poultry feed obtained in example 5 after feeding AA broiler chickens;
fig. 4 is a graph of myogastric tissue sections after a control AA broiler test.
Detailed Description
The technical scheme of the invention is described in detail through specific embodiments.
Example 1
A preparation method of feed for improving digestive tract function of poultry comprises the following steps:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 10:4, starting a ball mill to grind until the powder is sieved by a 100-mesh sieve, so as to obtain pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 30 ℃, and adding 2kg of effective living bacteria to 1.03X10 total 9 Fermenting cfu/g of composite bacteria for 5 hours, adding 40kg of silage bamboo shoot shell, 4kg of soybean meal, 10kg of rice bran, 10kg of peanut cake and 500kg of calcium chloride aqueous solution with concentration of 0.1mol/L, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 115 ℃ for 20 minutes, cooling to room temperature, pouring into a colloid mill, running for 20 minutes at a gap of 30 mu m, and running for 5 minutes at a gap of 15 mu m to obtain superfine crushed alkali grass roots;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 10:1:4:1:2, composing;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 40kg of corn, 1kg of bran, 8kg of rapeseed meal, 5kg of wheat, 5kg of shell powder and 1kg of kudzuvine root powder in a stirring state, homogenizing for 2 times under the pressure of 50MPa, wherein the inlet temperature of the homogenizer is 20 ℃, and obtaining premix;
s4, adding 1kg of taurine, 0.6kg of grape seed procyanidine, 1kg of calcium bicarbonate and 1kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the feed for improving the digestive tract function of poultry.
Example 2
A preparation method of feed for improving digestive tract function of poultry comprises the following steps:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 10:1, starting a ball mill to grind to 100 meshes, and obtaining pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 40 ℃, and adding 1kg of effective viable bacteria to 4.92 multiplied by 10 9 Fermenting cfu/g of composite bacteria for 2 hours, adding 30kg of silage bamboo shoot shell, 12kg of soybean meal, 4kg of rice bran, 20kg of peanut cake and 200kg of calcium chloride aqueous solution with concentration of 0.5mol/L, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 105 ℃ for 40 minutes, cooling to room temperature, pouring into a colloid mill, running for 40 minutes at a gap of 20 mu m, and running for 15 minutes at a gap of 10 mu m to obtain superfine crushed alkali grass roots;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 10:4:1:2:1, the composition is as follows;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 20kg of corn, 5kg of bran, 2kg of rapeseed meal, 15kg of wheat, 1kg of shell powder and 2kg of kudzuvine root powder in a stirring state, homogenizing for 4 times under the pressure of 30MPa, wherein the inlet temperature of the homogenizer is 10 ℃, and obtaining premix;
s4, adding 2kg of taurine, 0.1kg of grape seed procyanidine, 2kg of calcium bicarbonate and 0.1kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the feed for improving the digestive tract function of poultry.
Example 3
A preparation method of feed for improving digestive tract function of poultry comprises the following steps:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 10:3, starting a ball mill to grind until the powder is sieved by a 100-mesh sieve, so as to obtain pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 33 ℃, and adding 1.8kg of effective living bacteria to the total of 2.35 multiplied by 10 9 Fermenting cfu/g of composite bacteria for 4 hours, adding 33kg of silage bamboo shoot shell, 10kg of soybean meal, 6kg of rice bran, 18kg of peanut cake and 300kg of calcium chloride aqueous solution with concentration of 0.4mol/L, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 108 ℃ for 35min, cooling to room temperature, pouring into a colloid mill, running for 35min at a gap of 22 mu m and running for 12min at a gap of 12 mu m, and obtaining superfine crushed alkali grass roots;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 10:2:3:1.2:1.7 parts;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 25kg of corn, 4kg of bran, 4kg of rapeseed meal, 12kg of wheat, 2kg of shell powder and 1.7kg of radix puerariae powder in a stirring state, homogenizing for 3 times under the pressure of 35MPa, wherein the inlet temperature of the homogenizer is 17 ℃, and obtaining a premix;
s4, adding 1.3kg of taurine, 0.5kg of grape seed procyanidine, 1.3kg of calcium bicarbonate and 0.6kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the feed for improving the digestive tract function of poultry.
Example 4
A preparation method of feed for improving digestive tract function of poultry comprises the following steps:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 10:2, starting a ball mill to grind until the powder passes through a 100-mesh sieve to obtain pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 37 ℃, and adding 1.2kg of effective living bacteria to the total of 4.16X10 9 Fermenting cfu/g of composite bacteria for 3 hours, adding 37kg of silage bamboo shoot shell, 6kg of soybean meal, 8kg of rice bran, 12kg of peanut cake and 400kg of calcium chloride aqueous solution with concentration of 0.2mol/L, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 112 ℃ for 25 minutes, cooling to room temperature, pouring into a colloid mill, running for 25 minutes at a gap of 28 mu m, and running for 8 minutes at a gap of 14 mu m to obtain superfine crushed grass roots;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 10:3:2:1.8: 1.3;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 35kg of corn, 2kg of bran, 6kg of rapeseed meal, 8kg of wheat, 4kg of shell powder and 1.3kg of radix puerariae powder in a stirring state, homogenizing for 3 times under the pressure of 45MPa, wherein the inlet temperature of the homogenizer is 13 ℃, and obtaining a premix;
s4, adding 1.7kg of taurine, 0.2kg of grape seed procyanidine, 1.7kg of calcium bicarbonate and 0.2kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the feed for improving the digestive tract function of poultry.
Example 5
A preparation method of feed for improving digestive tract function of poultry comprises the following steps:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 4:1, starting a ball mill to grind to 100 meshes, and obtaining pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 35 ℃, and adding 1.5kg of effective living bacteria to the total of 3.30X10 9 Fermenting cfu/g of composite bacteria for 3.5 hours, adding 35kg of silage bamboo shoot shell, 8kg of soybean meal, 7kg of rice bran, 15kg of peanut cake and 350kg of calcium chloride aqueous solution with concentration of 0.3mol/L, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 110 ℃ for 30 minutes, cooling to room temperature, pouring into a colloid mill, running for 30 minutes at a 25 mu m gap and running for 10 minutes at a 13 mu m gap, and obtaining superfine crushed grass roots;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 20:5:5:3:3, composing;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 30kg of corn, 3kg of bran, 5kg of rapeseed meal, 10kg of wheat, 3kg of shell powder and 1.5kg of kudzu root powder in a stirring state, homogenizing for 3 times under the pressure of 40MPa, wherein the inlet temperature of the homogenizer is 15 ℃, and obtaining premix;
s4, adding 1.5kg of taurine, 0.35kg of grape seed procyanidine, 1.5kg of calcium bicarbonate and 0.4kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the feed for improving the digestive tract function of poultry.
Comparative example 1
A method for preparing poultry feed, comprising the steps of:
s1, crushing alkali grass, sieving with a 40-mesh sieve, placing the crushed alkali grass into a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen with the vacuum degree of 40kpa, and the mass-volume ratio g/L of the alkali grass to the liquid nitrogen of 4:1, starting a ball mill to grind until the powder passes through a 100-mesh sieve to obtain pretreated alkali grass;
s2, regulating the temperature of 100kg of pretreated alkali grass to 35 ℃, and adding 1.5kg of effective living bacteria to 3.30X10 total 9 Fermenting cfu/g of composite bacteria for 3.5h, adding 35kg of silage bamboo shoot shell, 8kg of soybean meal, 7kg of rice bran, 15kg of peanut cake and 350kg of calcium chloride aqueous solution with concentration of 0.3mol/L, mixing uniformly, sending into a hydrothermal reaction kettle, preserving heat at 110 ℃ for 30min, cooling to room temperature, pouring into a colloid mill, and running for 30m at a gap of 25 μmin, running for 10min with a 13 μm gap to obtain superfine crushed alkali grass;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 20:5:5:3:3, composing;
s3, adding 100kg of superfine crushed alkali grass into a homogenizer, sequentially adding 30kg of corn, 3kg of bran, 5kg of rapeseed meal, 10kg of wheat, 3kg of shell powder and 1.5kg of radix puerariae powder in a stirring state, homogenizing for 3 times under the pressure of 40MPa, wherein the inlet temperature of the homogenizer is 15 ℃, and obtaining a premix;
s4, adding 1.5kg of taurine, 0.35kg of grape seed procyanidine, 1.5kg of calcium bicarbonate and 0.4kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the poultry feed.
Comparative example 2
A method for preparing poultry feed, comprising the steps of:
s1, crushing waste grass roots and sieving the crushed grass roots with a 40-mesh sieve to obtain pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 35 ℃, and adding 1.5kg of effective living bacteria to the total of 3.30X10 9 Fermenting cfu/g of composite bacteria for 3.5 hours, adding 35kg of silage bamboo shoot shell, 8kg of soybean meal, 7kg of rice bran, 15kg of peanut cake and 350kg of calcium chloride aqueous solution with concentration of 0.3mol/L, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 110 ℃ for 30 minutes, cooling to room temperature, pouring into a colloid mill, running for 30 minutes at a 25 mu m gap and running for 10 minutes at a 13 mu m gap, and obtaining superfine crushed grass roots;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 20:5:5:3:3, composing;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 30kg of corn, 3kg of bran, 5kg of rapeseed meal, 10kg of wheat, 3kg of shell powder and 1.5kg of kudzu root powder in a stirring state, homogenizing for 3 times under the pressure of 40MPa, wherein the inlet temperature of the homogenizer is 15 ℃, and obtaining premix;
s4, adding 1.5kg of taurine, 0.35kg of grape seed procyanidine, 1.5kg of calcium bicarbonate and 0.4kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the poultry feed.
Comparative example 3
A method for preparing poultry feed, comprising the steps of:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 4:1, starting a ball mill to grind to 100 meshes, and obtaining pretreated grass roots;
s2, uniformly mixing 100kg of pretreated alkali grass roots with 35kg of silage bamboo shoot shells, 8kg of soybean meal, 7kg of rice bran, 15kg of peanut cakes and 350kg of calcium chloride aqueous solution with the concentration of 0.3mol/L, sending the mixture into a hydrothermal reaction kettle, preserving heat at 110 ℃ for 30min, cooling to room temperature, pouring the mixture into a colloid mill, running for 30min at a 25 mu m gap and running for 10min at a 13 mu m gap to obtain superfine crushed alkali grass roots;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 30kg of corn, 3kg of bran, 5kg of rapeseed meal, 10kg of wheat, 3kg of shell powder and 1.5kg of kudzu root powder in a stirring state, homogenizing for 3 times under the pressure of 40MPa, wherein the inlet temperature of the homogenizer is 15 ℃, and obtaining premix;
s4, adding 1.5kg of taurine, 0.35kg of grape seed procyanidine, 1.5kg of calcium bicarbonate and 0.4kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the poultry feed.
Comparative example 4
A method for preparing poultry feed, comprising the steps of:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 4:1, starting a ball mill to grind to 100 meshes, and obtaining pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 35 ℃, and adding 1.5kg of effective living bacteria to the total of 3.30X10 9 Fermenting cfu/g of compound bacteria for 3.5h, adding 35kg of silage bamboo shoot shell, 8kg of soybean meal, 7kg of rice bran, 15kg of peanut cake and 350kg of water, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 110 ℃ for 30min, cooling to room temperature, pouring into a colloid mill, running for 30min at a gap of 25 μm,running for 10min with 13 μm gap to obtain superfine crushed grass root;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 20:5:5:3:3, composing;
s3, adding 100kg of superfine crushed grass roots into a homogenizer, sequentially adding 30kg of corn, 3kg of bran, 5kg of rapeseed meal, 10kg of wheat, 3kg of shell powder and 1.5kg of kudzu root powder in a stirring state, homogenizing for 3 times under the pressure of 40MPa, wherein the inlet temperature of the homogenizer is 15 ℃, and obtaining premix;
s4, adding 1.5kg of taurine, 0.35kg of grape seed procyanidine, 1.5kg of calcium bicarbonate and 0.4kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the poultry feed.
Comparative example 5
A method for preparing poultry feed, comprising the steps of:
s1, crushing waste alkali grass roots, sieving with a 40-mesh sieve, placing the crushed waste alkali grass roots in a cavity of a ball mill, vacuumizing the cavity under sealing, spraying liquid nitrogen, wherein the mass-volume ratio g/L of the waste alkali grass roots to the liquid nitrogen is 4:1, starting a ball mill to grind to 100 meshes, and obtaining pretreated grass roots;
s2, regulating the temperature of 100kg of pretreated alkali grass roots to 35 ℃, and adding 1.5kg of effective living bacteria to the total of 3.30X10 9 Fermenting cfu/g of composite bacteria for 3.5 hours, adding 35kg of silage bamboo shoot shell, 8kg of soybean meal, 7kg of rice bran, 15kg of peanut cake and 350kg of calcium chloride aqueous solution with concentration of 0.3mol/L, uniformly mixing, sending into a hydrothermal reaction kettle, preserving heat at 110 ℃ for 30 minutes, cooling to room temperature, pouring into a colloid mill, running for 30 minutes at a 25 mu m gap and running for 10 minutes at a 13 mu m gap, and obtaining superfine crushed grass roots;
the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 20:5:5:3:3, composing;
s3, stirring 100kg of superfine crushed alkali grass roots, and sequentially adding 30kg of corn, 3kg of bran, 5kg of rapeseed meal, 10kg of wheat, 3kg of shell powder and 1.5kg of radix puerariae powder in a stirring state to obtain a premix;
s4, adding 1.5kg of taurine, 0.35kg of grape seed procyanidine, 1.5kg of calcium bicarbonate and 0.4kg of salt into 100kg of premix, uniformly stirring, and granulating to obtain the poultry feed.
1400 AA chickens with the age of 14 days are selected for the test, randomly divided into seven groups, and each group is repeated by 20 times, wherein 10 chickens are repeated for each group, the test period is 28 days, and the test of the age of 42 days of the chickens is ended.
Early 6 per day: 00 and late 6:00 feeds were performed, the control group feeds commercial feeds, the test group feeds the poultry feeds obtained in example 5 and comparative examples 1 to 5, and the remaining feeding conditions were kept consistent. The method comprises the following steps:
the weight and mortality of each group were as follows:
the slaughter performance of each group was as follows:
tu Tilv, percent | Semi-bore-free rate% | Full bore-free rate% | Chest muscle rate, percent | Leg muscle rate% | |
Example 5 | 96.91 | 86.15 | 77.84 | 25.62 | 20.98 |
Comparative example 1 | 96.42 | 85.87 | 77.15 | 25.37 | 20.77 |
Comparative example 2 | 95.73 | 86.51 | 78.21 | 24.54 | 21.06 |
Comparative example 3 | 95.50 | 86.78 | 78.46 | 24.18 | 21.56 |
Comparative example 4 | 96.17 | 86.03 | 77.53 | 25.06 | 19.36 |
Comparative example 5 | 95.96 | 86.36 | 77.92 | 24.83 | 20.54 |
Control group | 93.66 | 84.47 | 77.17 | 19.28 | 17.24 |
The weights of the myogastric and adenomatous groups were as follows:
28d myostomach, g | 28d adenoma stomach, g | 42d myostomach, g | 42d adenoma stomach, g | |
Example 5 | 30.126 | 6.056 | 33.396 | 7.621 |
Comparative example 1 | 30.073 | 6.010 | 33.289 | 7.476 |
Comparative example 2 | 29.931 | 5.814 | 32.247 | 8.219 |
Comparative example 3 | 29.803 | 5.795 | 32.033 | 8.426 |
Comparative example 4 | 29.992 | 5.941 | 33.019 | 7.941 |
Comparative example 5 | 29.945 | 5.886 | 32.645 | 8.003 |
Control group | 22.018 | 4.975 | 25.496 | 6.889 |
The digestive tract organ indexes of each group of broiler chickens are as follows:
the quality of pectoral muscle of each group of broiler chickens is as follows:
the quality of the meat drumsticks of each group is as follows:
flesh color L | Flesh color a | Flesh color b | PH value | |
Example 5 | 45.53 | 1.93 | 8.26 | 6.70 |
Comparative example 1 | 45.80 | 2.14 | 8.14 | 6.74 |
Comparative example 2 | 42.36 | 2.01 | 7.06 | 6.25 |
Comparative example 3 | 41.81 | 2.14 | 6.80 | 6.15 |
Comparative example 4 | 43.57 | 2.26 | 7.87 | 6.48 |
Comparative example 5 | 44.26 | 2.33 | 7.39 | 6.26 |
Control group | 45.55 | 4.46 | 6.89 | 6.64 |
From the above table, it can be seen that: the feed provided by the invention reduces the death rate and improves the weight and slaughtering performance of broilers; the feed provided by the invention has the advantages that the proportion of balancing myostomach and adenosis is always maintained at 4.38-4.97:1, the growth and development of digestive organs of the broiler chickens can be promoted, the digestive tract regulation and repair of the broiler chickens can be realized, and meanwhile, the quality of muscles (particularly pectoral muscle and leg muscles) can be improved.
Then, 20 of the groups of example 5 and the control group were randomly selected, and the myostomach and the adenostomachs were dissected and tissue-sectioned, and the results thereof are shown in fig. 1 to 4, and it was found that: example 5 group of seeds have normal papilla and stomach wall and moderate hand feeling; in the control group, the glandular stomach nipple was found to be flat even disappeared, the hand feel was hard, the glandular stomach wall was thickened, the glandular mucosa was swollen and thickened, and 2 cases of ulcerated ulcer foci were appeared, and three-degree infection was caused.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (5)
1. A method for preparing feed for improving digestive tract functions of poultry, which is characterized by comprising the following steps:
s1, crushing and sieving waste grass roots, ball-milling the waste grass roots by vacuum liquid nitrogen until the waste grass roots pass through a 100-mesh sieve, wherein the vacuum degree is 30-50kpa, and the mass-volume ratio g/L of the waste grass roots to the liquid nitrogen is 10:1-4, obtaining pretreated grass roots;
s2, regulating the temperature of the pretreated grass roots to 30-40 ℃, and adding 1-5 multiplied by 10 of the total number of effective living bacteria 9 cfuFermenting with composite bacteria for 2-5 hr, adding silage bamboo shoot shell, soybean meal, rice bran, peanut cake, and calcium chloride water solution, mixing, performing hydrothermal reaction at 105-115 deg.C for 20-40min, cooling to room temperature, and grinding to obtain superfine crushed grass root;
in S2, the composite bacteria are prepared from geotrichum candidum, saccharomycetes, rhizopus, bacillus subtilis and trichoderma viride according to the number ratio of the live bacteria of 10:1-4:1-4:1-2:1-2 parts;
in the S2, the concentration of the calcium chloride aqueous solution is 0.1-0.5mol/L, and the mass ratio of the pretreated alkali grass roots, the compound bacteria, the silage bamboo shoot shells, the soybean meal, the rice bran, the peanut cakes and the calcium chloride aqueous solution is 100:1-2:30-40:4-12:4-10:10-20:200-500;
s3, uniformly mixing superfine crushed grass roots, corns, bran, rapeseed meal, wheat, shell powder and kudzuvine root powder, and homogenizing at 30-50MPa to obtain a premix;
s3, homogenizing by using a homogenizer, wherein the inlet temperature of the homogenizer is 10-20 ℃;
s4, adding taurine, grape seed procyanidine, calcium bicarbonate and salt into the premix, uniformly stirring, and granulating to obtain feed for improving the digestive tract function of poultry;
the poultry is broiler chicken.
2. The method for preparing a feed for improving digestive tract functions of poultry according to claim 1, wherein in S2, grinding is performed by using a colloid mill, running for 20-40min with a gap of 20-30 μm, and running for 5-15min with a gap of 10-15 μm.
3. The method for preparing the feed for improving the digestive tract function of poultry according to claim 1, wherein in the S3, the mass ratio of the superfine crushed grass roots, corn, bran, rapeseed meal, wheat, shell powder and kudzuvine root powder is 100:20-40:1-5:2-8:5-15:1-5:1-2.
4. The method for preparing the feed for improving the digestive tract function of the poultry according to claim 1, wherein in the S4, the mass ratio of premix, taurine, grape seed procyanidine, calcium bicarbonate and salt is 100:1-2:0.1-0.6:1-2:0.1-1.
5. A feed for improving digestive tract function of poultry, characterized by being prepared by the feed preparation method for improving digestive tract function of poultry according to any one of claims 1 to 4.
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