CN113355221A - Microorganism constant temperature incubator - Google Patents
Microorganism constant temperature incubator Download PDFInfo
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- CN113355221A CN113355221A CN202110475188.5A CN202110475188A CN113355221A CN 113355221 A CN113355221 A CN 113355221A CN 202110475188 A CN202110475188 A CN 202110475188A CN 113355221 A CN113355221 A CN 113355221A
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- 244000005700 microbiome Species 0.000 title claims abstract description 23
- 239000011521 glass Substances 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 11
- 230000003287 optical effect Effects 0.000 claims abstract description 7
- 238000003860 storage Methods 0.000 claims description 7
- 230000007306 turnover Effects 0.000 claims description 7
- 238000011084 recovery Methods 0.000 claims description 6
- 230000007246 mechanism Effects 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 2
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 claims 1
- 239000010902 straw Substances 0.000 description 6
- 238000005070 sampling Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
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- Cell Biology (AREA)
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- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a microorganism constant-temperature incubator which comprises an incubator, wherein a temperature adjusting assembly is arranged in the incubator, a placing frame for placing a plurality of culture dishes, a suction pipe for sucking a sample from the culture dishes, a suction pipe pneumatic assembly, a first moving unit for driving the suction pipe to move and a slide preparation unit for preparing a slide are arranged in the incubator, and the slide preparation unit comprises a second moving unit for driving the slide preparation to move; a groove for inserting the glass slide is arranged in the incubator, a light source is arranged at the bottom of the groove, an observation lens assembly of the optical microscope extending into the groove is fixedly penetrated on the incubator, and an adjusting assembly for adjusting the brightness of the light source is also arranged outside the incubator; use the constant temperature incubator structure of this application, just can observe through optical microscope's observation lens subassembly outside the constant temperature incubator, can observe respectively to many culture dishes simultaneously, solved the difficult problem that meets at present.
Description
Technical Field
The invention relates to the technical field of constant-temperature culture of microorganisms, in particular to a constant-temperature incubator for microorganisms.
Background
The microorganism constant-temperature incubator is widely applied to scientific research departments such as medical treatment and health, medical industry, biochemistry, industrial production, agricultural science and the like, and mainly used for culturing various microorganisms or organisms such as tissues, cells and the like;
at present, for monitoring the state of microorganisms in a constant temperature box, the box is usually opened for sampling observation, and the microorganisms change due to great change of the environment in the sampling process to influence the final sampling observation data; some existing methods for arranging an electron microscope on an incubator have the following defects: 1. the realization is difficult, most of the concepts are just one concept, and the implementation is difficult; 2. the selection detection of multiple culture dishes cannot be dealt with; therefore, a microorganism constant temperature incubator convenient for observation is needed to solve the technical problem.
Disclosure of Invention
The technical problem to be solved by the present invention is to provide a microorganism incubator aiming at the above mentioned defects of the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a microbial constant-temperature incubator is constructed and comprises an incubator, wherein a temperature adjusting assembly is arranged in the incubator, a placing rack for placing a plurality of culture dishes, a suction pipe for sucking a sample from the culture dishes, a suction pipe pneumatic assembly, a first moving unit for driving the suction pipe to move and a slide preparation unit for preparing a slide are arranged in the incubator, and the slide preparation unit comprises a second moving unit for driving the slide preparation unit to move; the utility model discloses a glass slide's incubator, including incubator, be provided with the recess that supplies the slide to insert in the incubator, the bottom of recess is provided with the light source, the outside of incubator runs through to be fixed with and stretches into the observation lens subassembly of the optical microscope in the recess, the outside of incubator still is provided with the regulation subassembly and the main control system of regulation the luminance of light source.
The microorganism constant-temperature incubator comprises a slide storage channel, wherein two slides for manufacturing slides are longitudinally stacked in pairs and placed in the slide storage channel; the bottom end of the slide storage channel is provided with a transverse discharge channel for the paired removal of the slides; the second moving unit comprises push plates for pushing the slide out of the discharging channel in pairs and a linear driving mechanism for driving the push plates; the slide making unit further comprises an overturning unit for overturning and opening the upper slide in the pushed pair of slides; the groove is positioned on the moving route of the push plate.
According to the microorganism constant-temperature incubator, a blanking port is formed in one side, away from the second moving unit, of the groove; and a recovery container positioned right below the blanking port is arranged in the incubator.
The invention relates to a microorganism constant-temperature incubator, wherein an overturning unit comprises a clamping piece for clamping a glass slide and a first overturning motor for driving the glass slide to overturn.
The microorganism constant-temperature incubator comprises an incubator body, a placing frame, a slide unit, a slide plate and a slide plate, wherein the incubator body is provided with a placing frame, the placing frame is arranged on the placing frame, and the slide plate is arranged on the placing frame.
The microorganism constant-temperature incubator comprises an installation block, a turnover unit and a control unit, wherein the installation block is provided with a groove in the middle, and the turnover unit is arranged in the groove.
The microorganism constant-temperature incubator comprises a first moving unit, a second moving unit and a third moving unit, wherein the first moving unit comprises a clamping cylinder for clamping a suction pipe, a second overturning motor for driving the clamping cylinder to overturn longitudinally, a transverse moving assembly for driving the second overturning motor to transversely move, and a longitudinal moving assembly for driving the transverse moving assembly to longitudinally move.
The invention has the beneficial effects that: use the constant temperature incubator structure of this application, drive the straw through the first mobile unit of main control system control and remove to putting the detection culture dish department on the frame, remove to film-making unit department after absorbing the sample and make the slide glass, then remove the slide glass to the recess in by the second mobile unit, rely on the luminance of adjusting part regulation light source, just can observe through optical microscope's observation lens subassembly outside the constant temperature incubator, can observe respectively to many culture dishes simultaneously, the difficult problem that meets at present has been solved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be further described with reference to the accompanying drawings and embodiments, wherein the drawings in the following description are only part of the embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive efforts according to the accompanying drawings:
FIG. 1 is a sectional plan view of a microorganism incubator according to a preferred embodiment of the present invention;
FIG. 2 is a sectional view of a mounting block of a microorganism incubator according to a preferred embodiment of the present invention;
FIG. 3 is a schematic structural view of a turning unit of the incubator according to the preferred embodiment of the present invention;
FIG. 4 is a schematic block diagram of a microorganism incubator according to a preferred embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention.
The microorganism constant temperature incubator according to the preferred embodiment of the present invention, as shown in fig. 1 and referring to fig. 2-4, comprises an incubator 1, a temperature adjustment assembly is disposed in the incubator 1, a placement frame 2 for placing a plurality of culture dishes, a straw 3 for sucking a sample from the culture dishes, a straw pneumatic assembly 12 (providing an adsorption force for the straw), a first moving unit 4 for driving the straw 3 to move, and a slide preparation unit for preparing a slide are disposed in the incubator 1, and the slide preparation unit comprises a second moving unit 50 for driving the slide to move; a groove 10 for inserting a glass slide is arranged in the incubator 1, a light source 100 is arranged at the bottom of the groove 10, an observation lens assembly 6 of an optical microscope extending into the groove is fixedly penetrated outside the incubator 1, and an adjusting assembly 7 for adjusting the brightness of the light source 100 and a control host 8 are also arranged outside the incubator 1;
use the constant temperature incubator structure of this application, drive straw 3 through 8 control first mobile unit of main control system and remove to putting and will detect culture dish department on the frame 2, remove to film-making unit department after absorbing the sample and make the slide glass, then remove to the recess 10 in with the slide glass by second mobile unit 50, rely on the luminance of adjusting part 7 regulated light source 100, just can observe through optical microscope's observation lens subassembly 6 outside the constant temperature incubator, can observe respectively to many culture dishes simultaneously, the difficult problem that meets at present has been solved.
Preferably, the slide making unit includes a slide storing passage 51, and two slides 52 for making slides are longitudinally stacked in pairs in the slide storing passage 51; the bottom end of the slide storage channel 51 is provided with a transverse discharge channel 53 for moving slides out in pairs; the second moving unit 50 includes a pushing plate 500 which pushes the slide pair out of the discharging channel and a linear driving mechanism 501 which drives it; the slide making unit further includes an inverting unit 54 that inverts and opens the upper slide out of the pushed-out pair of slides; the groove 10 is positioned on the moving path of the push plate 500;
when the push plate 500 is driven to move by the linear driving mechanism, the two bottommost slides in the slide storage channel 51 are pushed out to the turning unit 54 from the discharge channel 53 once, the turning unit 54 drives the slides above to turn over and open, the first moving unit 4 drives the suction tube 3 to move to the area between the two slides, the suction tube pneumatic assembly blows air to the suction tube, a sample is pushed out of the suction tube 3 and falls onto the slide below, then the turning unit 54 drives the slides above to turn over and reset, the slide manufacturing is completed, then the push plate 500 pushes the slides to continue to advance, the slides enter the groove 10, the light source brightness can be started and adjusted at the moment, and the observation can be carried out through the observation lens assembly;
the linear driving mechanism can be realized by adopting the existing linear motor, screw rod and other equipment.
Preferably, a blanking port 101 is arranged on one side of the groove 10 facing away from the second moving unit; a recovery container 9 positioned right below the blanking port 101 is arranged in the incubator 1; the push plate 500 can conveniently continue to deduce the slide, so that the slide can be moved out of the blanking port and fall into the recovery container 9 for recovery; then, the push plate 500 is reset to wait for the next detection action.
Preferably, the reversing unit 54 includes a clamping member 540 for clamping the slide and a first reversing motor 541 for reversing the clamping member; reasonable and compact structure and convenient operation and control.
Preferably, the inner wall of the incubator 1 is convexly provided with a mounting block 11 which is arranged side by side with the placing frame 2, and the slide making unit is arranged on the mounting block 11; the middle of the mounting block 11 is provided with a concave groove 110, and the overturning unit 54 is arranged in the concave groove 110; the structure layout is reasonable, and the sampling, the glass slide making, the observation and the recovery action of a plurality of culture dishes can be conveniently executed.
Preferably, the first moving unit 4 comprises a clamping cylinder 40 for clamping the suction tube 3, a second overturning motor 41 for driving the clamping cylinder 40 to overturn longitudinally, a longitudinal moving assembly 42 for driving the second overturning motor 41 to move longitudinally, and a transverse moving assembly 43 for driving the longitudinal moving assembly to move transversely; the structure is simple, and the control is convenient.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (7)
1. A microorganism constant-temperature incubator comprises an incubator, wherein a temperature adjusting assembly is arranged in the incubator, and the incubator is characterized in that a placing rack for placing a plurality of culture dishes, a suction pipe for sucking a sample from the culture dishes, a suction pipe pneumatic assembly, a first moving unit for driving the suction pipe to move and a slide preparation unit for preparing a slide are arranged in the incubator, and the slide preparation unit comprises a second moving unit for driving the slide preparation to move; the incubator is internally provided with a groove for inserting the glass slide, the bottom of the groove is provided with a light source, the incubator is fixedly provided with an observation lens assembly of the optical microscope extending into the groove in a penetrating way, and the outer side of the incubator is also provided with an adjusting assembly for adjusting the brightness of the light source and a control host.
2. The microbiological incubator as claimed in claim 1, wherein said slide making unit comprises a slide storage channel inside which two slides for making slides are placed in longitudinal stacks in pairs; the bottom end of the slide storage channel is provided with a transverse discharge channel for the paired removal of the slides; the second moving unit comprises push plates for pushing the slide out of the discharging channel in pairs and a linear driving mechanism for driving the push plates; the slide making unit further comprises an overturning unit for overturning and opening the upper slide in the pushed pair of slides; the groove is positioned on the moving route of the push plate.
3. The microbial constant temperature incubator according to claim 2, wherein a side of the groove facing away from the second moving unit is provided with a blanking port; and a recovery container positioned right below the blanking port is arranged in the incubator.
4. The microbe constant-temperature incubator according to claim 2, wherein the overturning unit comprises a clamping piece for clamping the slide and a first overturning motor for driving the first overturning piece to overturn.
5. The microorganism incubator according to claim 2, wherein the inner wall of the incubator is provided with a mounting block in a protruding manner, the mounting block is arranged side by side with the placing frame, and the slide making unit is arranged on the mounting block.
6. The incubator according to claim 5, wherein the mounting block is provided at the center thereof with a concave groove, and the turn-over unit is disposed in the concave groove.
7. The incubator as claimed in any one of claims 1 to 6, wherein the first moving unit comprises a grip cylinder for gripping the pipette, a second turnover motor for driving the grip cylinder to turn vertically, a traverse unit for driving the second turnover motor to traverse, and a traverse unit for driving the traverse unit to move vertically.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110475188.5A CN113355221A (en) | 2021-04-29 | 2021-04-29 | Microorganism constant temperature incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110475188.5A CN113355221A (en) | 2021-04-29 | 2021-04-29 | Microorganism constant temperature incubator |
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| Publication Number | Publication Date |
|---|---|
| CN113355221A true CN113355221A (en) | 2021-09-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110475188.5A Pending CN113355221A (en) | 2021-04-29 | 2021-04-29 | Microorganism constant temperature incubator |
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| Country | Link |
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| CN (1) | CN113355221A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107026A (en) * | 2021-12-02 | 2022-03-01 | 深圳零一生命科技有限责任公司 | Growth curve monitor and monitoring method thereof |
| CN116970476A (en) * | 2023-07-25 | 2023-10-31 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Detection device and detection method for mycoplasma synoviae of chickens |
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| CN109490044A (en) * | 2018-12-25 | 2019-03-19 | 宁波察微生物科技有限公司 | A kind of mounting machine |
| CN212741393U (en) * | 2020-06-03 | 2021-03-19 | 康珞生物科技（武汉）有限公司 | Cell culture case with real-time detection and monitoring function |
| CN112592814A (en) * | 2020-12-23 | 2021-04-02 | 温州乔断信息科技有限公司 | Microorganism periodic sampling detection device and sampling method |
-
2021
- 2021-04-29 CN CN202110475188.5A patent/CN113355221A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| JP2010273574A (en) * | 2009-05-27 | 2010-12-09 | Nikon Corp | Pipette and carrying device |
| CN109490044A (en) * | 2018-12-25 | 2019-03-19 | 宁波察微生物科技有限公司 | A kind of mounting machine |
| CN212741393U (en) * | 2020-06-03 | 2021-03-19 | 康珞生物科技（武汉）有限公司 | Cell culture case with real-time detection and monitoring function |
| CN112592814A (en) * | 2020-12-23 | 2021-04-02 | 温州乔断信息科技有限公司 | Microorganism periodic sampling detection device and sampling method |
Non-Patent Citations (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107026A (en) * | 2021-12-02 | 2022-03-01 | 深圳零一生命科技有限责任公司 | Growth curve monitor and monitoring method thereof |
| CN116970476A (en) * | 2023-07-25 | 2023-10-31 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Detection device and detection method for mycoplasma synoviae of chickens |
| CN116970476B (en) * | 2023-07-25 | 2024-03-12 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Detection device and detection method for mycoplasma synoviae of chickens |
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Application publication date: 20210907 |