CN116970476A - Detection device and detection method for mycoplasma synoviae of chickens - Google Patents
Detection device and detection method for mycoplasma synoviae of chickens Download PDFInfo
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- CN116970476A CN116970476A CN202310916037.8A CN202310916037A CN116970476A CN 116970476 A CN116970476 A CN 116970476A CN 202310916037 A CN202310916037 A CN 202310916037A CN 116970476 A CN116970476 A CN 116970476A
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- 238000001514 detection method Methods 0.000 title claims abstract description 132
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 24
- 235000013330 chicken meat Nutrition 0.000 title description 12
- 241000202942 Mycoplasma synoviae Species 0.000 title description 2
- 239000007788 liquid Substances 0.000 claims abstract description 133
- 238000002156 mixing Methods 0.000 claims abstract description 44
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 35
- 210000002966 serum Anatomy 0.000 claims abstract description 33
- 238000002347 injection Methods 0.000 claims abstract description 24
- 239000007924 injection Substances 0.000 claims abstract description 24
- 238000004140 cleaning Methods 0.000 claims abstract description 21
- 241000204031 Mycoplasma Species 0.000 claims abstract description 20
- 241001260012 Bursa Species 0.000 claims abstract description 17
- 239000011550 stock solution Substances 0.000 claims description 16
- 230000008878 coupling Effects 0.000 claims description 10
- 238000010168 coupling process Methods 0.000 claims description 10
- 238000005859 coupling reaction Methods 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 9
- 230000005611 electricity Effects 0.000 claims description 7
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000003860 storage Methods 0.000 description 9
- 241000274582 Pycnanthus angolensis Species 0.000 description 5
- 238000009826 distribution Methods 0.000 description 5
- 239000011087 paperboard Substances 0.000 description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 4
- 235000017491 Bambusa tulda Nutrition 0.000 description 4
- 241001330002 Bambuseae Species 0.000 description 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 4
- 239000011425 bamboo Substances 0.000 description 4
- 244000144977 poultry Species 0.000 description 3
- 235000013594 poultry meat Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 244000309464 bull Species 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 210000003555 cloaca Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/35—Mycoplasma
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Abstract
The invention discloses a chicken bursa mycoplasma detection device, which comprises a detection box body with a cylindrical shape and a digital display control panel arranged on the outer side of the detection box body, wherein a plurality of injector placement holes for placing injectors are formed in the upper side of the detection box body, the injector placement holes are uniformly distributed by taking the axis as the center, an injection component for injecting the injectors is arranged in the middle of the upper side of the detection box body, a reagent adding component is arranged in the upper side of the detection box body, and operations of sequentially injecting different serum, mixing the serum and the reagent, automatically supplying the detection box, automatically replacing the detection box and the like can be realized through matching of the injection component, a cleaning component, a mixer, a telescopic liquid receiving component, a reagent adding component, the box supplying component and the like, so that the detection efficiency is greatly improved, and the chicken bursa mycoplasma detection device is suitable for large-scale cultivation.
Description
Technical Field
The invention relates to the field of xx equipment, in particular to a chicken bursa mycoplasma detection device and a detection method.
Background
Poultry mycoplasma mainly infects poultry and is widely parasitic in respiratory tracts, cloaca, digestive tracts, oviduct mucous membranes and joint sacs of the poultry, the disease occurs all the year round, but is more frequent in winter and spring, chickens in each day age are susceptible to infection, the susceptibility of the chickens is higher than that of adult chickens, and the resistance of the chickens can be enhanced along with the increase of the age.
The utility model provides a portable chicken bursa mycoplasma detection device and method of 202210137800.2, adopts primer mechanism to mix solution according to MS-F1 and MS-R1 primer sequence in first primer mixing box when the primer, pours into through the injection screwed pipe and stores in first primer mixing box, and the circular primer flow jet is carried out to a plurality of arc expands the stand pipe outward to the rethread, accomplishes first round primer operation like this.
However, most of the existing detection devices can only detect the detection in sequence, and the detection devices are manually operated by staff, so that the efficiency is low.
Disclosure of Invention
(one) solving the technical problems
The invention aims to solve the problems and provide a chicken bursa mycoplasma detection device and a detection method.
(II) technical scheme
In order to achieve the above purpose, the present invention provides the following technical solutions:
the invention provides a chicken bursa mycoplasma detection device, which comprises a detection box body with a cylindrical shape and a digital display control panel arranged on the outer side of the detection box body, wherein a plurality of syringe placement holes for placing syringes are formed in the upper side of the detection box body and uniformly distributed by taking an axis as the center, a pushing component for pushing the syringes is arranged in the middle of the upper side of the detection box body, a reagent adding component is arranged in the upper side of the detection box body, a mixer for mixing serum led out of each syringe and reagent led out of the reagent adding component is arranged below the reagent adding component, the mixers are respectively connected with the syringe placement holes through liquid guide pipes, a cleaning component for cleaning the liquid guide pipes is arranged in the detection box body, a box supplying component for supplying the detection boxes to the lower parts of the mixers and a box receiving component for collecting the detection boxes after the detection liquid is dripped through the mixers are respectively arranged below the detection box body, and a telescopic box receiving component for pushing the detection boxes is arranged between the box receiving components and the box receiving components.
Further, the detection box includes from lower box and the last box that supreme distribution set up down, and the border passes through extension board each other fixed connection between lower box and the last box, and two open slot that use the detection box axis as central symmetry distribution are seted up to the both sides of extension board, and every open slot department all articulates and is provided with the revolving cover, the upside of detection box can be dismantled and is provided with the top shroud, and the upside of top shroud is provided with the handle, be provided with the detection cavity in the lower box.
Further, the injection subassembly is including fixed the setting in the inside seat section of thick bamboo of detection box upside, and the seat section of thick bamboo sets up the axis department at the detection box, and the inside bottom surface of seat section of thick bamboo is fixed and is provided with the motor, the output axle head fixedly connected with of motor is first electric telescopic handle, and first electric telescopic handle rotates and sets up in the seat section of thick bamboo, the push rod head end of first electric telescopic handle wears out detection box upside surface and fixedly connected with bull stick, and the inside fixed second electric telescopic handle that is provided with of bull stick, the push rod orientation of second electric telescopic handle is kept away from first electric telescopic handle direction and fixedly connected with flexible push head, the flexible direction of push rod of second electric telescopic handle is unanimous with the radius of detection box, the downside fixedly connected with push rod of flexible push head, the lower extreme fixedly connected with of push rod is pushed the push head, the hole is placed to the syringe is provided with and is a plurality of concentric circle setting with the detection box axis as a plurality of, and every group is including the equiangular distribution's such as the center the detection box axis, and two sets of syringe of immediately adjacent syringe place the hole along the radial direction of motor of the detection box, the radial direction corresponds to the electric control panel, the output end of digital display is connected to the digital display respectively.
Further, every the lower extreme that the hole was placed to the syringe all is connected with the second coupling that is used for being connected with the catheter cooperation, is connected with the washing coupling on the second coupling, wash the subassembly including seting up the inside first stock solution cavity of lower box upside, lower box is close to upper portion one side of first stock solution cavity and is connected with the filling pipe, be provided with first liquid pump in the first stock solution cavity, be provided with the level sensor who is used for detecting its inside liquid level in the first stock solution cavity, the liquid outlet of first liquid pump is connected with the connecting pipe, the upper end of connecting pipe wears out first stock solution cavity and is connected with the annular liquid pipe that leads to that is the ring-shaped, annular liquid pipe is connected with each other through branch liquid pipe and each washing coupling respectively, digital display control panel's output electricity is connected to first liquid pump's input, level sensor's output electricity is connected to digital display control panel's input.
Further, the blender is including being the mixed casing of cylinder shape, the upper side surface of box under the upper end protrusion of mixed casing, the lower extreme protrusion of mixed casing extends to in the detection chamber, and mixed casing's inside is provided with mixing chamber and mixing duct from top to bottom respectively, mixing chamber and mixing duct communicate each other, mixed casing's upside is connected with a plurality of and is used for carrying out the first coupling that is connected with each catheter respectively, and first coupling communicates each other with mixing chamber, and mixing duct internal fixation is provided with the spiral floor, mixing duct's lower extreme opening and opening part are provided with out liquid shower nozzle.
Further, reagent adds the subassembly including seting up the inside middle part position of last box second stock solution cavity, the upside of going up the box is provided with the liquid feeding hole that is used for adding reagent to the inside of second stock solution cavity, is provided with sealed end cap in the liquid feeding hole, the inside bottom surface of second stock solution cavity is fixed to be provided with the second liquid pump, the liquid outlet of second liquid pump is connected with the one end of liquid feeding pipe, and the lower extreme of liquid feeding pipe is connected to mixed casing upside and communicates with mixed intracavity portion, digital display control panel's output electricity is connected to the input of second liquid pump.
Further, supply box subassembly including seting up the second lift cavity of lower box internal axis department, two second lift spouts that use it as central symmetry distribution are seted up to the both sides of second lift cavity, and the slip is provided with the second in second lift cavity and the second lift spout holds in the palm the box board, all rotates in every second lift spout and is provided with the second lead screw, the lower extreme of second lead screw is rotated by the second lead screw motor drive of fixed setting in lower box, the second lead screw in every second lift spout all holds in the palm box board threaded connection each other with the second, digital display control panel's output electricity is connected to the input of second lead screw motor.
Further, connect box subassembly including seting up the first lift cavity in second lift cavity one side, two first lift spouts that use it as central symmetry distribution are seted up to the both sides of first lift cavity, and the slip is provided with first box board that holds in the palm in first lift cavity and the first lift spout, all rotates in every first lift spout and is provided with first lead screw, the lower extreme of first lead screw is rotated by the first lead screw motor drive of fixed setting in lower box, the first lead screw in every first lift spout all with first box board threaded connection each other, digital display control panel's output electricity is connected to first lead screw motor's input.
Further, flexible liquid subassembly that connects is including the fixed second electric telescopic handle that sets up on detecting the cavity inside wall, and the push rod head end fixedly connected with of second electric telescopic handle connects the liquid funnel, connect the outer shape of liquid funnel wide down cone shape often, connect the inside cavity lower extreme opening of liquid funnel and opening part to be connected with the fluid-discharge tube, the fixed floor that is provided with of pipe on the push rod of second electric telescopic handle, the downside fixedly connected with of the fixed floor of pipe is used for pushing away the box board that pushes away to the detection box, digital display control panel's output electricity is connected to the input of second electric telescopic handle.
A detection method of a chicken bursa mycoplasma detection device comprises the following steps:
s1: serum samples are collected in the syringes, and each syringe with the collected serum samples is placed in a syringe placement hole respectively;
s2: the injection component performs injection on the injector in one of the injector placement holes, serum enters the mixer through the liquid guide tube, meanwhile, the reagent adding component adds reagent into the mixer, and the serum and the reagent enter the mixer for mixing at the same time and fall into the detection box supplied by the box supplying component from the mixer;
s3: after entering the blender through the catheter with the serum in S2, wash the subassembly and can realize wasing in the catheter and the blender, wash the waste liquid that produces and collect the discharge through the telescopic liquid receiving subassembly:
s4: after the mixed liquid falls into the detection box supplied by the box supplying assembly in the step S2, in the step S3, the telescopic liquid receiving assembly collects the waste liquid generated by cleaning and pushes the detection box which falls into the mixed liquid to the box receiving assembly for collection;
s5: and the injection component performs injection on the injector in the next injector placing hole, and the steps are repeated until the serum detection of the injectors in all the injector placing holes is finished.
(III) beneficial effects
Compared with the prior art, the invention has the beneficial effects that:
1. the operations of sequentially carrying out bolus injection, mixing of serum and a reagent, automatic supply of a detection box, automatic replacement of the detection box and the like on different serum can be realized through the cooperation of a bolus injection component, a cleaning component, a mixer, a telescopic liquid receiving component, a box receiving component, a reagent adding component, a box supplying component and the like, so that the uninterrupted automatic detection of multiple serum is realized, the detection efficiency is greatly improved, and the method is suitable for mycoplasma detection under large-scale cultivation;
2. the telescopic liquid receiving component can realize automatic replacement of the detection box, connection between the box supplying component and the box receiving component, and can also realize the box pushing operation and simultaneously receive and discharge of waste liquid, so that the functional structure is simplified;
3. the cleaning component can clean the liquid guide tube and the mixer, and can perform the effect of avoiding cross contamination after the completion of mixing and detecting the serum in one of the syringes so as not to influence the detection of the serum in the subsequent next syringe.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the front view of the present invention;
FIG. 2 is a schematic diagram of the left-hand construction of FIG. 1 according to the present invention;
FIG. 3 is a schematic view of the cross-sectional structure of FIG. 1 A-A in accordance with the present invention;
FIG. 4 is a schematic view of the cross-sectional B-B structure of FIG. 2 according to the present invention;
FIG. 5 is a schematic view of the cross-sectional C-C structure of FIG. 2 in accordance with the present invention;
FIG. 6 is a schematic view of a partially enlarged structure of the invention at D of FIG. 3;
FIG. 7 is a schematic view of a partially enlarged structure of FIG. 3 at E in accordance with the present invention;
FIG. 8 is a schematic view of the present invention in a partially enlarged configuration at F of FIG. 3;
FIG. 9 is a schematic view of a partially enlarged structure of the present invention at G of FIG. 3;
FIG. 10 is a schematic view of the structure of the present invention shown at H in FIG. 3 in a partially enlarged manner;
FIG. 11 is a schematic perspective view of the structure of FIG. 1 according to the present invention;
FIG. 12 is a schematic view of another directional perspective of FIG. 1 of the present invention;
fig. 13 is a schematic view of a partially enlarged structure at J of fig. 11 according to the present invention.
The reference numerals are explained as follows: 1. detecting a box body; 101. a lower box body; 102. a top cover; 103. an open slot; 104. a rotary cover; 105. an upper case; 106. a support plate; 107. a detection chamber; 2. a bolus assembly; 201. a seat cylinder; 202. a motor; 203. a first electric telescopic rod; 204. a rotating rod; 205. a second electric telescopic rod; 206. a telescopic push head; 207. pushing the pressing rod; 208. pushing the pressure head; 209. a guide rod; 210. a guide groove; 3. a catheter; 4. cleaning the assembly; 401. a first reservoir chamber; 402. a first liquid pump; 403. a connecting pipe; 404. a liquid level sensor; 405. a liquid adding tube; 406. an annular liquid-passing pipe; 407. a liquid supporting pipe; 5. a mixer; 501. a mixing housing; 502. a first pipe joint; 503. a mixing chamber; 504. mixing pore canal; 505. a spiral rib plate; 506. a liquid outlet nozzle; 6. a telescopic liquid receiving component; 601. a second electric telescopic rod; 602. a tube fixing rib plate; 603. a liquid receiving funnel; 604. a liquid discharge pipe; 605. a pushing box plate; 7. a box receiving assembly; 701. a first elevation chamber; 702. a first tray deck; 703. a first lead screw motor; 704. a first lifting chute; 705. a first lead screw; 706. a movable supporting plate; 707. positioning columns; 708. a baffle; 709. a handle; 8. a syringe placement hole; 801. a second pipe joint; 802. flushing the pipe joint; 9. a reagent addition assembly; 901. a second reservoir chamber; 902. a second liquid pump; 903. a liquid adding tube; 904. sealing the end cap; 10. a digital display control panel; 11. a cartridge supply assembly; 1101. a second elevation chamber; 1102. a second tray deck; 1103. a second lead screw motor; 1104. the second lifting chute; 1105. a second lead screw; 12. a detection box; 13. a syringe.
Detailed Description
For the purpose of the present invention; the technical scheme and advantages are more clear, and the technical scheme of the invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, based on the examples herein, which are within the scope of the invention as defined by the claims, will be within the scope of the invention as defined by the claims.
Referring to fig. 1-13, the invention provides a chicken bursa mycoplasma detection device, which comprises a detection box body 1 with a cylindrical shape and a digital display control panel 10 arranged on the outer side of the detection box body 1, wherein a plurality of injector placement holes 8 for placing injectors 13 are uniformly distributed on the upper side of the detection box body 1 by taking an axis as a center, as shown in fig. 3 and 11 of the specification, needles of the injectors 13 are downwards inserted into a second pipe joint 801, push handle ends of the injectors 13 are upwards and are used for pushing in cooperation with a pushing head 208 of a pushing assembly 2, the injector placement holes 8 are provided with a plurality of groups which are concentrically arranged by taking the central axis of the detection box body 1 as the center, each group comprises a plurality of injector placement holes 8 uniformly distributed by taking the central axis of the detection box body 1 as the center, and two adjacent groups of injector placement holes 8 are in one-to-one correspondence along the radial direction of the detection box body 1.
The middle position of the upper side of the detection box body 1 is provided with a pushing injection component 2 for pushing the injector 13, the inside of the upper side of the detection box body 1 is provided with a reagent adding component 9, the lower side of the reagent adding component 9 is provided with a mixer 5 for mixing serum led out from each injector 13 and reagent led out from the reagent adding component 9, the mixer 5 is respectively connected with each injector placing hole 8 through a liquid guide tube 3, a cleaning component 4 for cleaning the liquid guide tube 3 is arranged in the detection box body 1, the lower side of the inner part of the detection box body 1 is respectively provided with a box supplying component 11 for supplying a detection box 12 to the lower side of the mixer 5 and a box receiving component 7 for collecting the detection box 12 after dripping the detection liquid is detected through the mixer 5, and a telescopic liquid receiving component 6 for receiving the cleaning waste liquid and pushing the detection box 12 from the box supplying component 11 to the box receiving component 7 is arranged between the box receiving component 7 and the box supplying component 11.
As shown in fig. 2, 11 and 13 of the specification, the detection box 1 comprises a lower box 101 and an upper box 105 which are distributed from bottom to top, edges between the lower box 101 and the upper box 105 are fixedly connected with each other through a support plate 106, two open slots 103 which are symmetrically distributed by taking a central axis of the detection box 1 as a center are formed in two sides of the support plate 106, a rotating cover 104 is hinged to each open slot 103, after the rotating cover 104 is opened, a worker can detach and replace the liquid guide tube 3 through the open slots 103, a top cover 102 is detachably arranged on the upper side of the detection box 1, a handle is arranged on the upper side of the top cover 102, and a detection chamber 107 is arranged in the lower box 101.
Referring to fig. 3, 11 and 12 of the specification, the injection assembly 2 includes a seat barrel 201 fixedly disposed in an upper side of the detection box 1, the seat barrel 201 is disposed at a central axis of the detection box 1, a motor 202 is fixedly disposed on an inner bottom surface of the seat barrel 201, an output shaft end of the motor 202 is fixedly connected with a first electric telescopic rod 203, the first electric telescopic rod 203 is rotatably disposed in the seat barrel 201, a push rod head end of the first electric telescopic rod 203 penetrates out of an upper side surface of the detection box 1 and is fixedly connected with a rotary rod 204, a second electric telescopic rod 205 is fixedly disposed in the rotary rod 204, a push rod of the second electric telescopic rod 205 faces a direction far away from the first electric telescopic rod 203 and is fixedly connected with a telescopic push head 206, a push rod telescopic direction of the push rod of the second electric telescopic rod 205 is consistent with a radial direction of the detection box 1, a push rod 207 is fixedly connected with a push rod 207 at a lower side of the telescopic push rod 206, a push rod 208 is fixedly connected with a lower end of the push rod 207, and an output end of the digital display control panel 10 is respectively electrically connected to an input end of the motor 202 and the second electric telescopic rod 205. Through the above-mentioned specific structural design, the injection subassembly 2 plays to carry out the injection to syringe 13 in the syringe placing hole 8, and wherein second electric telescopic handle 205 is used for adjusting the radial position of pushing and pressing head 208 along detection box 1 for can realize carrying out the injection to syringe 13 in the syringe placing hole 8 along the radial different positions of detection box 1, can realize the automated inspection in batches to serum.
As shown in fig. 4 and 6 of the specification, the lower end of each syringe placement hole 8 is connected with a second pipe joint 801 which is used for being connected with the liquid guide pipe 3 in a matching way, a flushing pipe joint 802 is connected to the second pipe joint 801, the cleaning assembly 4 comprises a first liquid storage cavity 401 which is formed inside the upper side of the lower box body 101, a liquid adding pipe 405 is connected to one side of the lower box body 101, which is close to the upper part of the first liquid storage cavity 401, a first liquid pump 402 is arranged in the first liquid storage cavity 401, a liquid level sensor 404 used for detecting the liquid level inside the first liquid storage cavity 401 is arranged in the first liquid storage cavity 401, a liquid outlet of the first liquid pump 402 is connected with a connecting pipe 403, the upper end of the connecting pipe 403 penetrates out of the first liquid storage cavity 401 and is connected with an annular liquid passing pipe 406, the annular liquid passing pipe 406 is connected with each flushing pipe joint 802 through a branch liquid pipe 407, the output end of the digital display control panel 10 is electrically connected to the input end of the first liquid pump 402, and the output end of the liquid level sensor 404 is electrically connected to the input end of the digital display control panel 10. In practical application, the cleaning component 4 is used for cleaning the catheter 3 and the mixer 5, and is used for avoiding cross-contamination after mixing and detecting serum in one of the syringes 13, so as not to affect the detection of serum in the next syringe 13.
As shown in fig. 4, 7 and 8 of the specification, the mixer 5 comprises a mixing housing 501 in a cylindrical shape, the upper end of the mixing housing 501 protrudes from the upper side surface of the lower housing 101, the lower end of the mixing housing 501 protrudes and extends into the detection chamber 107, a mixing cavity 503 and a mixing duct 504 are respectively arranged in the mixing housing 501 from top to bottom, the mixing cavity 503 and the mixing duct 504 are communicated with each other, a plurality of first pipe joints 502 for respectively connecting with the liquid guide pipes 3 are connected to the upper side of the mixing housing 501, the first pipe joints 502 are communicated with the mixing cavity 503, a spiral rib 505 is fixedly arranged in the mixing duct 504, and a liquid outlet nozzle 506 is arranged at the opening of the lower end of the mixing duct 504. By the above specific structural design, the spiral rib 505 can achieve sufficient mixing of serum and reagents.
Referring to fig. 4 and 11 of the specification, the reagent adding assembly 9 includes a second liquid storage chamber 901 formed at a middle position inside the upper case 105, a liquid adding hole for adding reagent into the second liquid storage chamber 901 is formed at a top side of the upper case 105, a sealing end cap 904 is disposed in the liquid adding hole, a second liquid pump 902 is fixedly disposed at an inner bottom surface of the second liquid storage chamber 901, a liquid outlet of the second liquid pump 902 is connected with one end of a liquid adding pipe 903, a lower end of the liquid adding pipe 903 is connected to an upper side of the mixing housing 501 and is communicated with the inside of the mixing chamber 503, and an output end of the digital display control panel 10 is electrically connected to an input end of the second liquid pump 902.
The box feeding assembly 11 comprises a second lifting chamber 1101 arranged at the central axis of the lower box 101, two second lifting sliding grooves 1104 which are symmetrically distributed by taking the second lifting chamber 1101 as a center are arranged on two sides of the second lifting chamber 1101, second box supporting plates 1102 are slidably arranged in the second lifting chamber 1101 and the second lifting sliding grooves 1104, second lead screws 1105 are rotatably arranged in each second lifting sliding groove 1104, the lower ends of the second lead screws 1105 are driven to rotate by second lead screw motors 1103 fixedly arranged in the lower box 101, the second lead screws 1105 in each second lifting sliding groove 1104 are in threaded connection with the second box supporting plates 1102, and the output ends of the digital display control panel 10 are electrically connected to the input ends of the second lead screw motors 1103. Through the above-mentioned concrete structural design, supply box subassembly 11 to be used for realizing the supply to detecting box 12, cooperation bolus subassembly 2, catheter 3, cleaning assembly 4, flexible connect liquid subassembly 6 and connect box subassembly 7 etc. to realize the uninterrupted automated inspection to the serum.
The box receiving assembly 7 comprises a first lifting chamber 701 arranged on one side of a second lifting chamber 1101, two first lifting sliding grooves 704 which are symmetrically distributed by taking the first lifting chamber 701 as a center are arranged on two sides of the first lifting chamber 701, a first box supporting plate 702 is arranged in the first lifting chamber 701 and the first lifting sliding grooves 704 in a sliding mode, a first lead screw 705 is arranged in each first lifting sliding groove 704 in a rotating mode, the lower end of the first lead screw 705 is driven to rotate by a first lead screw motor 703 fixedly arranged in the lower box body 101, the first lead screw 705 in each first lifting sliding groove 704 is in threaded connection with the first box supporting plate 702, and the output end of the digital display control panel 10 is electrically connected to the input end of the first lead screw motor 703.
The telescopic liquid receiving assembly 6 comprises a second electric telescopic rod 601 fixedly arranged on the inner side wall of the detection chamber 107, a liquid receiving funnel 603 is fixedly connected to the head end of a push rod of the second electric telescopic rod 601, the outer shape of the liquid receiving funnel 603 is of a cone shape which is wide at the top and narrow at the bottom, a liquid discharge pipe 604 is connected to the opening of the hollow lower end of the liquid receiving funnel 603, a pipe fixing rib plate 602 is fixedly arranged on the push rod of the second electric telescopic rod 601, a box pushing plate 605 for pushing the detection box 12 is fixedly connected to the lower side of the pipe fixing rib plate 602, and the output end of the digital display control panel 10 is electrically connected to the input end of the second electric telescopic rod 601.
Working principle:
serum samples are collected in the syringes 13, and the respective syringes 13 with the collected serum samples are placed in the syringe placement holes 8, respectively; the injection component 2 performs injection on the injector 13 in one of the injector placement holes 8, serum enters the mixer 5 through the liquid guide tube 3, meanwhile, the reagent adding component 9 adds reagent into the mixer 5, and the serum and the reagent enter the mixer 5 for mixing at the same time and fall into the detection box 12 supplied by the box supplying component 11 from the mixer 5; after the mixed solution enters the mixer 5 through the liquid guide tube 3, the cleaning component 4 can clean the liquid guide tube 3 and the mixer 5, and waste liquid generated by cleaning is collected and discharged through the telescopic liquid receiving component 6: after the mixed liquid falls into the detection box 12 supplied by the box supplying assembly 11, the telescopic liquid receiving assembly 6 collects the waste liquid generated by cleaning, and meanwhile, the detection box 12 falling into the mixed liquid is pushed to the box receiving assembly 7 for collection; the injection component 2 performs injection on the syringe 13 in the next syringe placement hole 8, and the above operation is repeated until all the syringes 13 in the syringe placement holes 8 are completely detected in serum.
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A chicken synovial sac mycoplasma detection device is characterized in that: the device comprises a detection box body (1) with a cylindrical shape and a digital display control panel (10) arranged on the outer side of the detection box body (1), wherein a plurality of syringe placement holes (8) which are uniformly distributed by taking the central axis as the center and used for placing syringes (13) are formed in the upper side of the detection box body (1), a pushing component (2) used for pushing the syringes (13) is arranged in the middle of the upper side of the detection box body (1), a reagent adding component (9) is arranged in the upper side of the detection box body (1), a mixer (5) used for mixing serum exported by each syringe (13) and reagent exported by the reagent adding component (9) is arranged below the reagent adding component (9), the mixer (5) is connected with each syringe placement hole (8) by a liquid guide tube (3) respectively, a cleaning component (4) used for cleaning the liquid guide tube (3) is arranged in the detection box body (1), a reagent adding component (9) is arranged below the detection box body (1) respectively, a liquid dropping component (12) used for supplying the detection box (12) to the mixer (5) to the mixer (11) is used for dropping the detection box (1) after the detection box (1) is mixed by the component (7), the telescopic liquid receiving assembly (6) used for carrying the cleaning waste liquid and pushing the detection box (12) from the box supplying assembly (11) to the box receiving assembly (7) is arranged between the box receiving assembly (7) and the box supplying assembly (11).
2. The chicken bursa mycoplasma detection device according to claim 1, wherein: the detection box body (1) comprises a lower box body (101) and an upper box body (105) which are arranged from bottom to top, edges between the lower box body (101) and the upper box body (105) are fixedly connected with each other through support plates (106), two open slots (103) which are symmetrically distributed by taking the central axis of the detection box body (1) as the center are formed in two sides of the support plates (106), a rotating cover (104) is hinged to each open slot (103), a top cover cap (102) is detachably arranged on the upper side of the detection box body (1), a handle is arranged on the upper side of the top cover cap (102), and a detection cavity (107) is formed in the lower box body (101).
3. The chicken bursa mycoplasma detection device according to claim 1, wherein: the injection assembly (2) comprises a seat cylinder (201) fixedly arranged in the upper side of the detection box body (1), the seat cylinder (201) is arranged at the central axis of the detection box body (1), a motor (202) is fixedly arranged on the inner bottom surface of the seat cylinder (201), a first electric telescopic rod (203) is fixedly connected to the output shaft end of the motor (202), the first electric telescopic rod (203) is rotatably arranged in the seat cylinder (201), a rotating rod (204) is fixedly connected to the upper side surface of the detection box body (1) in a penetrating mode, a second electric telescopic rod (205) is fixedly arranged in the rotating rod (204), a pushing rod of the second electric telescopic rod (205) faces towards the direction far away from the first electric telescopic rod (203) and is fixedly connected with a telescopic pushing head (206), the pushing rod (207) is fixedly connected to the lower side of the telescopic head (206), the lower end of the pushing rod (207) is fixedly connected with a plurality of central axis (8) of the detection boxes (1) in a concentric mode, the central axis (1) is uniformly distributed in groups, the central axis (1) is uniformly distributed in a concentric mode, two groups of immediately adjacent injector placement holes (8) are in one-to-one correspondence along the radial direction of the detection box body (1), and the output end of the digital display control panel (10) is electrically connected to the input ends of the motor (202) and the second electric telescopic rod (205) respectively.
4. The chicken bursa mycoplasma detection device according to claim 1, wherein: every the lower extreme that hole (8) was placed to the syringe all is connected with second coupling (801) that are used for being connected with catheter (3) cooperation, is connected with flushing pipe joint (802) on second coupling (801), wash subassembly (4) including seting up first stock solution cavity (401) in lower box (101) upside inside, lower box (101) are close to upper portion one side of first stock solution cavity (401) and are connected with filling pipe (405), be provided with first liquid pump (402) in first stock solution cavity (401), be provided with in first stock solution cavity (401) and be used for carrying out liquid level sensor (404) that detects its inside liquid level, the liquid outlet of first liquid pump (402) is connected with connecting pipe (403), first stock solution cavity (401) are worn out and are connected with annular liquid pipe (406) that are circular, liquid pipe (406) are connected with each other through branch liquid pipe (407) respectively, digital display control panel's (10) output to digital display panel's input (407) of digital display control panel's input (10) electric liquid level sensor (404).
5. The chicken bursa mycoplasma detection device according to claim 1, wherein: the mixer (5) is including being the mixed casing (501) of cylinder shape, the upper side surface of box (101) under the upper end protrusion of mixed casing (501), the lower extreme protrusion of mixed casing (501) extends to in detecting chamber (107), and the inside of mixed casing (501) is provided with mixing chamber (503) and mixing duct (504) from last to lower respectively, mixing chamber (503) and mixing duct (504) communicate each other, the upside of mixed casing (501) is connected with a plurality of first coupling (502) that are used for being connected with each catheter (3) respectively, and first coupling (502) communicate each other with mixing chamber (503), mixing duct (504) internal fixation is provided with spiral floor (505), the lower extreme opening and the opening part of mixing duct (504) are provided with liquid shower nozzle (506).
6. The chicken bursa mycoplasma detection device according to claim 5, wherein: the reagent adds subassembly (9) including seting up second stock solution cavity (901) in the inside middle part position of last box (105), the upside of going up box (105) is provided with the liquid feeding hole that is used for adding reagent to the inside of second stock solution cavity (901), is provided with sealed end cap (904) in the liquid feeding hole, the inside bottom surface of second stock solution cavity (901) is fixed to be provided with second liquid pump (902), the liquid outlet of second liquid pump (902) is connected with the one end of liquid feeding pipe (903), and the lower extreme of liquid feeding pipe (903) is connected to mixed casing (501) upside and communicates with mixed cavity (503) inside, the output electricity of digital display control panel (10) is connected to the input of second liquid pump (902).
7. The chicken bursa mycoplasma detection device according to claim 1, wherein: the box feeding assembly (11) comprises a second lifting chamber (1101) formed in the inner central axis of the lower box body (101), two second lifting sliding grooves (1104) which are symmetrically distributed by taking the second lifting chamber (1101) as the center are formed in two sides of the second lifting chamber (1101), second box supporting plates (1102) are arranged in the second lifting chamber (1101) and the second lifting sliding grooves (1104) in a sliding mode, second lead screws (1105) are arranged in each second lifting sliding groove (1104) in a rotating mode, the lower ends of the second lead screws (1105) are driven to rotate by second lead screw motors (1103) fixedly arranged in the lower box body (101), the second lead screws (1105) in each second lifting sliding groove (1104) are in threaded connection with the second box supporting plates (1102), and the output end of the digital display control panel (10) is electrically connected to the input end of each second lead screw motor (1103).
8. The chicken bursa mycoplasma detection device according to claim 7, wherein: the box receiving assembly (7) comprises a first lifting chamber (701) arranged on one side of a second lifting chamber (1101), two first lifting sliding grooves (704) which are symmetrically distributed by taking the first lifting chamber (701) as a center are arranged on two sides of the first lifting chamber (701), first box supporting plates (702) are arranged in the first lifting chamber (701) and the first lifting sliding grooves (704) in a sliding mode, first lead screws (705) are arranged in each first lifting sliding groove (704) in a rotating mode, the lower ends of the first lead screws (705) are driven to rotate by first lead screw motors (703) fixedly arranged in the lower box body (101), the first lead screws (705) in each first lifting sliding groove (704) are in threaded connection with the first box supporting plates (702), and the output end of the digital display control panel (10) is electrically connected to the input end of each first lead screw motor (703).
9. The chicken bursa mycoplasma detection device according to claim 1, wherein: the telescopic liquid receiving assembly (6) comprises a second electric telescopic rod (601) fixedly arranged on the inner side wall of the detection chamber (107), a liquid receiving funnel (603) is fixedly connected to the head end of a push rod of the second electric telescopic rod (601), the shape of the liquid receiving funnel (603) is in a cone shape with a wide top and a narrow bottom, a liquid discharging pipe (604) is connected to the opening of the hollow lower end of the liquid receiving funnel (603), a pipe fixing rib plate (602) is fixedly arranged on the push rod of the second electric telescopic rod (601), a box pushing plate (605) for pushing the detection box (12) is fixedly connected to the lower side of the pipe fixing rib plate (602), and the output end of the digital display control panel (10) is electrically connected to the input end of the second electric telescopic rod (601).
10. The detection method of the chicken bursa mycoplasma detection device according to claim 1, wherein the detection method comprises the following steps: the method comprises the following steps:
s1: serum samples are collected in the syringes (13), and each syringe (13) with the collected serum samples is placed in the syringe placement hole (8) respectively;
s2: the injection component (2) performs injection on the injector (13) in one of the injector placement holes (8), serum enters the mixer (5) through the liquid guide tube (3), meanwhile, the reagent adding component (9) adds reagent into the mixer (5), the serum and the reagent enter the mixer (5) for mixing at the same time, and the serum falls into the detection box (12) supplied by the box supplying component (11) through the mixer (5);
s3: after entering into blender (5) through catheter (3) with the serum in S2, wash subassembly (4) can realize wasing in catheter (3) and blender (5), wash the waste liquid that produces and collect the discharge through telescopic liquid receiving subassembly (6):
s4: after the mixed liquid falls into the detection box (12) supplied by the box supplying assembly (11) in the step S2, in the step S3, the telescopic liquid receiving assembly (6) collects waste liquid generated by cleaning and pushes the detection box (12) after the mixed liquid falls into the box receiving assembly (7) for collection;
s5: the injection component (2) carries out injection on the injector (13) in the next injector placing hole (8), and the steps are repeated until the serum detection of the injectors (13) in all the injector placing holes (8) is finished.
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