CN113354483A - Composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof - Google Patents

Composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof Download PDF

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CN113354483A
CN113354483A CN202110784097.XA CN202110784097A CN113354483A CN 113354483 A CN113354483 A CN 113354483A CN 202110784097 A CN202110784097 A CN 202110784097A CN 113354483 A CN113354483 A CN 113354483A
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powder
parts
composition
release layer
composite
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江国托
单春乔
刘艳
刘秋晨
王效禹
顾艳丽
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Dalian Sanyi Animal Drug Co ltd
Shandong Heze Sanyi Bio Engineering Co ltd
Jiangsu Sanyi Bioengineering Co ltd
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Dalian Sanyi Animal Drug Co ltd
Shandong Heze Sanyi Bio Engineering Co ltd
Jiangsu Sanyi Bioengineering Co ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/40Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Soil Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Fertilizers (AREA)

Abstract

The invention belongs to the technical field of organic fertilizers, and particularly relates to a composition for producing a biological organic fertilizer by fermenting livestock and poultry manure and a preparation method thereof. The composite core comprises a composite core and a sustained-release layer, wherein the sustained-release layer wraps the composite core, and the composite core comprises a composite enzyme and a composite bacterium powder. The composite core includes: alkaline protease, lipase, cellulase, pectinase, bacillus subtilis powder, bacillus megaterium powder, nitrogen-fixing bacillus GD powder, JF bacillus, enterococcus faecalis powder, candida powder and rhodopseudomonas palustris powder, wherein the slow release layer consists of hydroxypropyl methyl cellulose, alginate and polyanhydride. The composition disclosed by the invention can be used for stably and quickly carrying out the process of producing the bio-organic fertilizer by fermenting the livestock and poultry manure, avoiding the influence on the activity of biological enzymes and flora due to the non-constant temperature in the fermentation process, and meanwhile, effectively retaining the nutrient components in the livestock and poultry manure and providing more nutrients for crops.

Description

Composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof
Technical Field
The invention belongs to the technical field of organic fertilizers, and particularly relates to a composition for producing a biological organic fertilizer by fermenting livestock and poultry manure and a preparation method thereof.
Background
With the development of modern agriculture and the popularization of the use of chemical fertilizers, the excessive application of the chemical fertilizers in agricultural production causes the degradation of soil quality and the obvious reduction of soil fertility, seriously influences the yield of crops, increases the demand of the chemical fertilizers increasingly, improves the agricultural production cost, forms vicious circle, thereby causing the problems of the reduction of the quality of agricultural products, even the deterioration of ecological environment and the like, and threatens the food safety and the environmental safety of China. In the modern agricultural production, in order to promote its production efficiency, reduce the influence of using the waste material to the surrounding environment, regard the main fertilizer of production with the fertilizer gradually to the use of fertilizer is also very useful to agricultural self.
The agricultural wastes refer to discarded residues in agricultural production, are large in quantity and various in types, mainly contain a large amount of organic matters in the agricultural wastes such as livestock and poultry manure and crop straws, and particularly contain high organic matter content and abundant protein, cellulose and the like in the crop straws and the livestock and poultry manure. At present, the environmental protection problem of livestock and poultry farms is increasingly severe, fermentation often needs to occupy a large area, the internal oxygen is insufficient, the stirring is difficult, heat is easy to dissipate, the fermentation temperature is not constant, the growth and the propagation of fermentation strains are influenced, and the fermentation period is prolonged.
Patent document CN101463334A discloses a fermentation liquid composition for preparing a bio-organic fertilizer, and a preparation method and application thereof, wherein the fermentation liquid composition is composed of bacillus subtilis fermentation liquid, bacillus pumilus fermentation liquid and pseudomonas fluorescens fermentation liquid. The invention also provides a preparation method of the fermentation liquid composition, which comprises the steps of respectively and independently fermenting the bacillus subtilis, the bacillus pumilus and the pseudomonas fluorescens, and then mixing the obtained fermentation liquids. The product has optimized strain combination and realizes multiple functions. The selected strains not only have growth promoting effect, but also have antagonistic effect on various plant soil-borne diseases; the bacillus subtilis and the pseudomonas fluorescens in the product are plant growth-promoting rhizobacteria, can excite and induce the systemic resistance of crops, improve the autoimmunity and enhance the disease resistance, but the fermentation speed is not controllable, and the conditions of overheating and the like are easily caused.
Patent document CN110627536A discloses an organic fertilizer fermentation composition, an organic fertilizer starter and an organic fertilizer fermentation method, wherein the active bacteria of the organic fertilizer fermentation composition are composed of bacillus subtilis, yeast and lactic acid bacteria. The organic fertilizer fermentation composition provided by the invention obviously shortens the fermentation time of the organic fertilizer, obviously reduces the odor in the fermentation process, and also realizes that the organic matter, moisture and sanitary indexes of the organic fertilizer are all higher than the national standard requirements, but the fermentation speed is uncontrollable, and the overheating and other conditions are easily caused.
Disclosure of Invention
The invention aims to provide a composition for producing a bio-organic fertilizer by fermenting livestock and poultry manure.
In order to achieve the purpose, the invention adopts the following technical scheme: the composition for producing the bio-organic fertilizer by fermenting the livestock and poultry manure is characterized by comprising a composite core and a slow release layer, wherein the slow release layer is wrapped outside the composite core, and the composite core comprises a composite enzyme and composite bacterium powder.
Preferably, the compound enzyme comprises the following components in parts by weight: 3-5 parts of alkaline protease, 1-3 parts of lipase, 1-3 parts of cellulase and 1-3 parts of pectinase.
Preferably, the composite bacterial powder comprises the following components in parts by weight: 5-10 parts of bacillus subtilis powder, 3-6 parts of bacillus megaterium powder, 3-6 parts of azotobacterium GD powder, 1-5 parts of JF bacillus powder, 3-6 parts of enterococcus faecalis powder, 1-5 parts of candida powder and 1-5 parts of rhodopseudomonas palustris powder.
Preferably, the total enzyme activity of the composite core is 30-50 ten thousand U/g, and the total number of viable bacteria is 1012~1013CFU/Kg。
Preferably, the slow release layer consists of hydroxypropyl methyl cellulose, alginate and polyanhydride.
Further, the weight parts ratio of the hydroxypropyl methyl cellulose, the alginate and the polyanhydride is (3-5): (1-2): (1-3).
In addition, the invention also provides a preparation method of the composition, which comprises the following steps:
s1, preparation of a slow release layer: dissolving hydroxypropyl methyl cellulose with the formula amount in NaOH aqueous solution, adding alginate, polyanhydride and a cross-linking agent with the formula amount under the ice-bath stirring condition, slowly adding, reacting for 6-10 h at room temperature, neutralizing with dilute hydrochloric acid, stirring for 30-60 min, centrifuging to remove bubbles, pouring into a culture dish, then putting into an oven, drying at 40-60 ℃, and finally freeze-drying to obtain a slow-release layer product with a double-layer membrane structure;
s2, wrapping the composite core by the slow release layer: and (3) mixing the compound enzyme and the compound bacteria according to the formula amount to obtain a compound core, spraying the solution obtained by dissolving the slow release layer product prepared in the step S1 on the compound core, and freeze-drying to obtain the composition for producing the bio-organic fertilizer by fermenting the livestock and poultry manure.
Further, the crosslinking agent in step S1 is glutaraldehyde or epichlorohydrin.
Further, in the step S1, the mass-to-volume ratio of the hydroxypropyl methylcellulose to the NaOH aqueous solution is 1: 2-5, g: mL, the mass-volume ratio of the alginate to the cross-linking agent is 1: 0.1-0.3, g: and (mL).
Further, the concentration of the NaOH aqueous solution in the step S1 is 10 mg/mL.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention ferments the livestock and poultry manure by the complex enzyme and the complex bacteria, which is beneficial to the reservation of elements such as phosphorus, nitrogen, potassium, carbon and the like in the livestock and poultry manure, so that the organic fertilizer obtained by final fermentation has better nutrients and is beneficial to the growth of crops.
(2) The arrangement of the slow release layer can effectively control the fermentation speed, avoid the reduction of flora activity and the inactivation of complex enzyme caused by too fast fermentation and non-constant fermentation temperature, and the toxic and inhibition effects of the change of the pH value and the moisture of the fermentation environment on the complex strains and the complex enzyme.
(3) The slow release layer provides a better environment for the complex enzyme and the complex bacteria by wrapping the complex core, the utilization rate and the survival rate of the complex enzyme and the complex bacteria are enhanced, strains and biological enzymes which still survive after the fermentation are remained in the organic fertilizer obtained by the fermentation, the continuous activity in the soil further enhances the effective nutrients of the soil, improves the rhizosphere microorganisms of crops, and improves the disease and insect resistance of plants.
Detailed Description
The present invention will be described in further detail with reference to the following examples. It should not be understood that the scope of the above-described subject matter of the present invention is limited to the following examples.
Example 1A composition for fermenting livestock and poultry manure to produce a bio-organic fertilizer and a preparation method thereof
The formula is as follows: 300g of hydroxypropyl methyl cellulose, 100g of alginate and 100g of polyanhydride;
complex enzyme: 300g of alkaline protease, 100g of lipase, 100g of cellulase and 100g of pectinase;
compound bacteria: 500g of bacillus subtilis powder, 300g of bacillus megaterium powder, 300g of nitrogen-fixing bacillus GD powder, 100g of JF bacillus powder, 300g of enterococcus faecalis powder, 100g of candida powder and 100g of rhodopseudomonas palustris powder.
The preparation method comprises the following steps:
s1, preparation of a slow release layer: dissolving hydroxypropyl methylcellulose with the formula amount in 600mL of 10mg/mL NaOH aqueous solution, adding alginate, polyanhydride and 10mL of epoxy chloropropane with the formula amount under the condition of ice-bath stirring, slowly adding, reacting at room temperature for 8h, neutralizing with dilute hydrochloric acid, stirring for 30min, centrifuging to remove bubbles, pouring into a culture dish, then placing into an oven to dry at 40 ℃, and finally freeze-drying to obtain a slow-release layer product with a double-layer membrane structure;
s2, wrapping the composite core by the slow release layer: and (3) mixing the compound enzyme and the compound bacteria according to the formula amount to obtain a compound core, spraying the solution obtained by dissolving the slow release layer product prepared in the step S1 on the compound core, and freeze-drying to obtain the composition for producing the bio-organic fertilizer by fermenting the livestock and poultry manure.
Example 2A composition for fermenting livestock and poultry manure to produce bio-organic fertilizer and a preparation method thereof
The formula is as follows: 400g of hydroxypropyl methyl cellulose, 100g of alginate and 200g of polyanhydride;
complex enzyme: 400g of alkaline protease, 200g of lipase, 200g of cellulase and 200g of pectinase;
compound bacteria: 800g of bacillus subtilis powder, 500g of bacillus megaterium powder, 500g of nitrogen-fixing bacillus GD powder, 300g of JF bacillus powder, 500g of enterococcus faecalis powder, 300g of candida powder and 300g of rhodopseudomonas palustris powder.
The preparation method comprises the following steps:
s1, preparation of a slow release layer: dissolving hydroxypropyl methyl cellulose with the formula amount in 1200mL of 10mg/mL NaOH aqueous solution, adding alginate, polyanhydride and 20mL of epoxy chloropropane with the formula amount under the condition of ice-bath stirring, slowly adding, reacting for 6 hours at room temperature, neutralizing with dilute hydrochloric acid, stirring for 40 minutes, centrifuging to remove bubbles, pouring into a culture dish, then placing into an oven to dry at 50 ℃, and finally freeze-drying to obtain a slow-release layer product with a double-layer membrane structure;
s2, wrapping the composite core by the slow release layer: and (3) mixing the compound enzyme and the compound bacteria according to the formula amount to obtain a compound core, spraying the solution obtained by dissolving the slow release layer product prepared in the step S1 on the compound core, and freeze-drying to obtain the composition for producing the bio-organic fertilizer by fermenting the livestock and poultry manure.
Example 3A composition for fermenting livestock and poultry manure to produce a bio-organic fertilizer and a preparation method thereof
The formula is as follows: 500g of hydroxypropyl methyl cellulose, 200g of alginate and 300g of polyanhydride;
complex enzyme: 500g of alkaline protease, 300g of lipase, 300g of cellulase and 300g of pectinase;
compound bacteria: 1000g of bacillus subtilis powder, 600g of bacillus megaterium powder, 600g of nitrogen-fixing bacillus GD powder, 500g of JF bacillus powder, 600g of enterococcus faecalis powder, 500g of candida powder and 500g of rhodopseudomonas palustris powder.
The preparation method comprises the following steps:
s1, preparation of a slow release layer: dissolving hydroxypropyl methyl cellulose with the formula amount in 1000mL of 10mg/mL NaOH aqueous solution, adding alginate, polyanhydride and 60mL of epoxy chloropropane with the formula amount under the condition of ice-bath stirring, slowly adding, reacting for 10 hours at room temperature, neutralizing with dilute hydrochloric acid, stirring for 60 minutes, centrifuging to remove bubbles, pouring into a culture dish, then placing into an oven to dry at 60 ℃, and finally freeze-drying to obtain a slow-release layer product with a double-layer membrane structure;
s2, wrapping the composite core by the slow release layer: and (3) mixing the compound enzyme and the compound bacteria according to the formula amount to obtain a compound core, spraying the solution obtained by dissolving the slow release layer product prepared in the step S1 on the compound core, and freeze-drying to obtain the composition for producing the bio-organic fertilizer by fermenting the livestock and poultry manure.
Comparative example 1, composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof
The comparative example differs from example 2 only in that: no polyanhydride is present.
The preparation method refers to example 2.
Comparative example 2, composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof
The comparative example differs from example 2 only in that: does not contain alginate.
The preparation method refers to example 2.
Comparative example 3, composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof
The comparative example differs from example 2 only in that: contains no hydroxypropyl methyl cellulose, alginate and polyanhydride.
The preparation method refers to example 2.
Comparative example 4, composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof
The preparation was carried out according to the method described in example 1 of patent document CN 110627536A.
Test example I measurement of fermentation results
First, experimental sample
Compositions prepared in examples 1-3 and comparative examples 1-4 of the present invention
Second, Experimental methods
2.1 composting method
The solid organic wastes for breeding are selected, livestock and poultry manure is taken as the main material, and straws are added to prepare the organic fertilizer by fermentation (the adding amount of the straws is 30 percent), and the nutrient properties of the organic fertilizer are shown in Table 1. Then respectively inoculating 10% of the compositions prepared in the invention in the embodiments 1-3 and the comparative examples 1-4, controlling the water content to be about 50%, stacking and fermenting under natural conditions, and manually turning the stacks after recording the temperature every afternoon; and stopping turning the pile after maintaining the high temperature for 8-10 days.
TABLE 1 Primary nutrient Properties of the heap Material
Figure BDA0003158086820000051
2.2 composting time and sampling
During the stacking process, the material temperature was recorded at 8:00 am and 5:00 pm every day.
2.3 organic fertilizer determination index
One part of the fresh compost sample is used for measuring the number of viable bacteria and the total number of bacillus in the fresh compost sample, the other part of the fresh compost sample is added with distilled water according to the proportion of 1:5 (volume ratio), the mixture is oscillated for 30min and centrifuged at 4000r/min, then supernatant is taken, and the total amount of organic substances and the total amount of humic acid in the air-dried sample are measured.
Third, experimental results
3.1 temperature Change during Stacking
The results of the experiment are shown in table 2.
TABLE 2 temperature Change during Stacking
Figure BDA0003158086820000061
As can be seen from the data in table 2, the temperature of the composition prepared in the embodiments 1 to 3 of the present invention changes during the composting process, the temperature of the materials in the stacking process stably rises, the temperature exceeds 40 ℃ at day 1 and day 2 respectively, and keeps a high level at day 4 to day 10, after the composting stops turning, the compost can still naturally release heat and continuously maintain a high temperature period for 2 to 4 days, and the temperature gradually decreases to room temperature after 16 days; the data of the examples 1-3 and the comparative examples 1-3 show that the comparative examples 1-3 are in a state of rapid fermentation and high temperature at the beginning of fermentation due to lack of the slow release layer, and the fermentation temperature is kept higher for 4-16 d and cannot be reduced, so that the fermentation period is prolonged under the condition of obtaining the same amount of organic fertilizer finally; from the experimental data of the comparative example 4, it can be known that the composition of the comparative example 4 is different from the composition and preparation method of the fermented composition, and although the composition can keep a higher fermentation temperature as in the examples 1 to 3 of the present invention, the maintenance time is short, and the organic matters in the livestock and poultry manure cannot be completely decomposed, so that it can be proved that the composite core is coated with the slow release layer to facilitate the fermentation of the composite bacteria and the composite enzyme, and the organic matters in the livestock and poultry manure can be effectively decomposed.
3.2 viable count after completion of composting
The results of the experiment are shown in table 3.
TABLE 3 viable cell count after completion of composting
Figure BDA0003158086820000071
The data in table 3 show that the compositions prepared in examples 1 to 3 of the present invention can still maintain a high viable count after composting, and the data results of examples 1 to 3 and comparative examples 1 to 4 show that the slow release layer wrapped outside the composite core of the present invention can provide a good fermentation environment for the composite flora, and can ensure that the composite bacteria entering the livestock and poultry manure and participating in fermentation maintain a high and stable fermentation efficiency, and the non-participating composite bacteria can still maintain a high activity in the slow release layer after fermentation is finished, and can enter the soil of crops as one of the nutrients of the fermented organic fertilizer, thereby further improving the rhizosphere microorganisms of the crops and enhancing the disease and insect resistance of the plants.
3.3 matter Change in piled Material
The results of the experiments are shown in tables 4 and 5.
TABLE 4 Change in the Main organic matter in the Stack Material
Figure BDA0003158086820000072
Figure BDA0003158086820000081
TABLE 5 change in humic acid content during stacking (in carbon)
Figure BDA0003158086820000082
It can be known from the data in table 4 that the compositions prepared in examples 1 to 3 of the present invention have a high degradation rate for organic substances contained in fermented livestock and poultry manure after fermentation, and it can be known from the data in examples 1 to 3 and comparative examples 1 to 4 that the slow release film wrapped on the outer layer of the composite core of the present invention can effectively improve the activity of the composite flora, so that the fermentation process is stably performed, the influence of conditions such as excessive temperature on the activity of the composite core is avoided, and the degradation rate for organic substances contained in fermented livestock and poultry manure is further improved.
As can be seen from the data in Table 5, the compositions prepared in the embodiments 1 to 3 of the present invention have high humic acid content in the initial stage and the final stage of fermentation, and the increase rates of the humic acid content in the whole fermentation process are respectively 13.8%, 14.6% and 13.4%, and the data in the embodiments 1 to 3 and the comparative examples 1 to 4 prove that the addition of the slow release substance of the present invention can significantly increase the humic acid content in the fermentation process, and increase the fertilizer efficiency of the organic fertilizer.
From the above experiments, it can be known that the composition prepared in embodiments 1 to 3 of the present invention can effectively enhance the fermentation efficiency and enhance the decomposition rate of organic components in feces, and at the same time, the slow release layer wrapped on the composite core can effectively control the stable fermentation process and provide a good fermentation condition for the activity of the strains and the biological enzymes in the composite core, so that a large amount of viable strains and biological enzymes still remain in the organic fertilizer after the fermentation is completed, and the strains and biological enzymes act on the soil of crops, thereby further improving rhizosphere microorganisms of the crops and improving the disease and insect resistance of the plants. The composition has high fermentation efficiency, needs less amount compared with other fermented products, has shorter fermentation period and is beneficial to saving the production cost.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (9)

1. The composition for producing the bio-organic fertilizer by fermenting the livestock and poultry manure is characterized by comprising a composite core and a slow release layer, wherein the slow release layer is wrapped outside the composite core, and the composite core comprises a composite enzyme and composite bacterium powder.
2. The composition as claimed in claim 1, wherein the complex enzyme comprises the following components in parts by weight: 3-5 parts of alkaline protease, 1-3 parts of lipase, 1-3 parts of cellulase and 1-3 parts of pectinase.
3. The composition as claimed in claim 1, wherein the composite bacterial powder comprises the following components in parts by weight: 5-10 parts of bacillus subtilis powder, 3-6 parts of bacillus megaterium powder, 3-6 parts of azotobacterium GD powder, 1-5 parts of JF bacillus powder, 3-6 parts of enterococcus faecalis powder, 1-5 parts of candida powder and 1-5 parts of rhodopseudomonas palustris powder.
4. The composition of claim 1, wherein the total enzyme activity of the composite core is 30-50 ten thousand U/g, and the total number of viable bacteria is 1012~1013CFU/Kg。
5. The composition of claim 1, wherein the sustained release layer is comprised of hydroxypropyl methylcellulose, alginate, and polyanhydride.
6. The composition according to claim 5, wherein the weight parts ratio of the hydroxypropyl methylcellulose, the alginate and the polyanhydride is (3-5): (1-2): (1-3).
7. A process for the preparation of a composition according to any one of claims 1 to 6, comprising the steps of:
s1, preparation of a slow release layer: dissolving hydroxypropyl methyl cellulose with the formula amount in NaOH aqueous solution, adding alginate, polyanhydride and a cross-linking agent with the formula amount under the ice-bath stirring condition, slowly adding, reacting for 6-10 h at room temperature, neutralizing with dilute hydrochloric acid, stirring for 30-60 min, centrifuging to remove bubbles, pouring into a culture dish, then putting into an oven, drying at 40-60 ℃, and finally freeze-drying to obtain a slow-release layer product with a double-layer membrane structure;
s2, wrapping the composite core by the slow release layer: and (3) mixing the compound enzyme and the compound bacteria according to the formula amount to obtain a compound core, spraying the solution obtained by dissolving the slow release layer product prepared in the step S1 on the compound core, and freeze-drying to obtain the composition for producing the bio-organic fertilizer by fermenting the livestock and poultry manure.
8. The method of claim 7, wherein the cross-linking agent in step S1 is glutaraldehyde or epichlorohydrin.
9. The method for preparing the composition according to claim 7, wherein the mass-to-volume ratio of the hydroxypropyl methylcellulose to the aqueous NaOH solution in the step S1 is 1: 2-5, g: mL, the mass-volume ratio of the alginate to the cross-linking agent is 1: 0.1-0.3, g: and (mL).
CN202110784097.XA 2021-07-12 2021-07-12 Composition for producing bio-organic fertilizer by fermenting livestock and poultry manure and preparation method thereof Pending CN113354483A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107500884A (en) * 2017-09-11 2017-12-22 大连三仪动物药品有限公司 Compound formulation for fermented livestock excrement production biological organic fertilizer
CN109020684A (en) * 2018-08-09 2018-12-18 长沙昱旻信息科技有限公司 A kind of sustained release bacillus subtilis microbial agent

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107500884A (en) * 2017-09-11 2017-12-22 大连三仪动物药品有限公司 Compound formulation for fermented livestock excrement production biological organic fertilizer
CN109020684A (en) * 2018-08-09 2018-12-18 长沙昱旻信息科技有限公司 A kind of sustained release bacillus subtilis microbial agent

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Application publication date: 20210907