CN113348987A - Efficient germination method for water lily seeds - Google Patents
Efficient germination method for water lily seeds Download PDFInfo
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- CN113348987A CN113348987A CN202110796676.6A CN202110796676A CN113348987A CN 113348987 A CN113348987 A CN 113348987A CN 202110796676 A CN202110796676 A CN 202110796676A CN 113348987 A CN113348987 A CN 113348987A
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- 241000209490 Nymphaea Species 0.000 title claims abstract description 72
- 235000016791 Nymphaea odorata subsp odorata Nutrition 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 33
- 230000035784 germination Effects 0.000 title abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 77
- 239000004745 nonwoven fabric Substances 0.000 claims abstract description 54
- 239000010802 sludge Substances 0.000 claims abstract description 41
- 239000004576 sand Substances 0.000 claims abstract description 39
- 239000002689 soil Substances 0.000 claims abstract description 29
- 239000000203 mixture Substances 0.000 claims abstract description 13
- 238000005286 illumination Methods 0.000 claims description 11
- 238000004140 cleaning Methods 0.000 claims description 9
- 235000013399 edible fruits Nutrition 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 8
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 230000007226 seed germination Effects 0.000 claims description 3
- 239000003864 humus Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 6
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 241000195493 Cryptophyta Species 0.000 abstract description 3
- 241000256113 Culicidae Species 0.000 abstract description 3
- 238000002955 isolation Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000009331 sowing Methods 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 241000209477 Nymphaeaceae Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000255925 Diptera Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a water lily seed high-efficiency germination method, which is characterized in that garden soil and activated sludge are placed at the bottom layer of a non-woven fabric seedling bag, then a mixture of seeds and sand is placed, the water level is timely reduced after the seeds germinate, and the seeds are prevented from directly contacting the activated sludge and the external environment by utilizing the physical isolation effect of the sand, so that the problem that the water quality requirement of the water lily sowing and breeding technology is high is solved in a breakthrough manner, and the current situation that the seedling rate is low due to the influence of mosquito larvae, algae and the like after the seeds germinate is effectively solved; by adopting the germination method, the germination rate of the water lily can be improved, and the seedling rate can be improved to more than 80%; the method has the advantages of high seedling rate, low cost and simple operation, and the seeds and the soil do not need to be sterilized and can be widely applied to field or indoor seedling culture.
Description
Technical Field
The invention relates to the technical field of aquatic plant cultivation, in particular to a high-efficiency germination method of water lily seeds.
Background
Nymphaea spp is a perennial aquatic plant of Nymphaeaceae (Nymphaeaceae) Nymphaeaceae, and is widely applied to cut flowers, potted flowers, water landscape construction and ecological restoration engineering due to beautiful flower types, rich flower colors and unique flavor. Meanwhile, the water lily is called as beauty sleeping in flowers, is pursued by gardening enthusiasts all over the world and is a favorite for aquatic flower exhibition at home and abroad. More than 50 kinds of water lily original strains exist in the world, are mainly distributed in tropical, subtropical and temperate regions, and are originally produced in 5 kinds in China. At present, after long-term breeding by breeders in various countries, thousands of water lily existing varieties are obtained.
The water lily propagation method comprises seed propagation, plant division propagation and tissue culture rapid propagation, but the tissue culture rapid propagation technology is not popularized and applied due to the difficulties in explant disinfection, callus induction and the like. In addition, the propagation coefficient of the plant division propagation is low, and the seed propagation technology is the most widely applied technology in production and breeding work at present. However, the seedlings of water lily in the planting and breeding process are weak in the initial stage, are easily affected by external factors such as illumination, water quality, microorganisms and soil nutrition, and are easily damaged by mosquitoes and chironomid larvae.
The invention patent (patent number CN103461134A) of Chinese invention discloses a method for raising the seedling rate to 80% by improving the germination method, but the method has higher requirement on the cultivation environment of water lily.
The utility model discloses a chinese utility model patent (patent number CN210694847U) a royal lotus, high-efficient germination apparatus of water lily seed discloses and improves the germination rate through improving seed treatment method and germination apparatus, and nevertheless the water lily seedling initial stage is more delicate, and when placing natural environment with the water lily seedling that will adopt the device to cultivate, receives external factor's influence easily, and water lily seedling survival rate is low.
In order to solve the series of problems, the invention overcomes the defects of the prior art, invents a seedling raising technology with high germination rate and seedling rate, simple operation and low cost, does not need to carry out sterile treatment on seeds and a matrix, realizes the seedling raising rate of the water lily seeds by propagating to be more than 80% in a breakthrough way, can effectively promote the conservation of endangered species of the water lily, and is widely applied to crossbreeding, production and propagation.
Disclosure of Invention
Aiming at the problems, the invention provides a high-efficiency germination method of water lily seeds.
The invention discloses a high-efficiency germination method of water lily seeds, which comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds for later use;
s2, seedling culture: mixing garden soil and activated sludge, putting the mixture into a non-woven fabric seedling raising bag, mixing sand with the seeds collected in the step S1, uniformly scattering the mixture into the non-woven fabric seedling raising bag, covering the surface with the sand, and transferring the non-woven fabric seedling raising bag into a water holding device for culture; garden soil, activated sludge, seeds and sand in the non-woven fabric seedling raising bags form a culture system;
s3, seedling management: placing the water containing device in a scattered light environment at 20-30 ℃; in the initial stage of culture, the water level of the water containing device is controlled to be higher than the culture system in the non-woven fabric seedling raising bag; after the seeds germinate, controlling the water surface of the water containing device to be lower than or equal to the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Furthermore, the height of the mixture of the garden soil and the activated sludge is 30-50% of the height of the non-woven fabric seedling raising bag.
Further, the weight ratio of the garden soil to the activated sludge is 5-9: 1.
furthermore, the activated sludge is humic sludge with the solid content of 2-4%, the sludge is mixed with garden soil according to a proportion, sufficient nutrients can be provided for seeds, the germination rate of the seeds is improved, and if the solid content is too low, water quality can be polluted during germination culture, and the water lily seed development is not facilitated.
Furthermore, the non-woven fabric seedling raising bag is a 20-18 non-woven fabric seedling raising bag.
Further, the seeds and sand were mixed in a weight ratio of 1: 1000-1500.
Further, the thickness of the sand coating is 2-3 mm.
Furthermore, the height of the water containing device is more than 15cm higher than the culture system in the non-woven fabric seedling raising bag.
Further, the illumination intensity of the scattered light is 2000Lux-2500Lux, and the illumination intensity is suitable for the germination of the water lily seeds.
Furthermore, the water level of the water containing control device is 1-6cm higher than the culture system in the non-woven fabric seedling raising bag.
Further, the water containing device includes, but is not limited to, a basin, a tank, and a pool.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, garden soil and activated sludge are placed on the bottom layer of the non-woven fabric seedling raising bag, and then the mixture of seeds and sand is placed, so that the water level is timely reduced after the seeds germinate, and the physical isolation effect of the sand is utilized to prevent the seeds from directly contacting the activated sludge and the external environment, so that the problem that the water quality requirement of the water lily sowing and breeding technology is high is solved in a breakthrough manner, and the current situation that the seedling rate is low due to the influence of mosquito larvae, algae and the like after the seeds germinate is effectively solved; by adopting the germination method, the germination rate of the water lily can be improved, and the seedling rate can be improved to more than 80%; the method has the advantages of high seedling rate, low cost and simple operation, and the seeds and the soil do not need to be sterilized and can be widely applied to field or indoor seedling culture.
According to the invention, sand and seeds are mixed, and the proportion of the sand and the seeds is adjusted, so that the seeds can fully absorb nutrients in the bottom garden soil and the activated sludge under the condition of physical isolation from the outside.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The culture system is an integral body formed by garden soil, activated sludge, seeds and sand in a non-woven fabric seedling raising bag after broadcasting and sand covering.
Example 1
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2.5 percent;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Example 2
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 5: 1, the mixture of garden soil and activated sludge is 50% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1000:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 18cm higher than that of a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2%;
s3, seedling management: placing the water containing device in an environment with the temperature of 20 ℃ and the illumination intensity of scattered light of 2500 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 1cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be level with the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Example 3
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 4%;
s3, seedling management: placing the water containing device in an environment with the temperature of 23 ℃ and the intensity of scattered light illumination of 2000 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Example 4
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and return sludge according to the proportion of 9: 1, the height of the mixture of garden soil and return sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the height of the covered sand is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than or equal to the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Comparative example 1
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: putting garden soil into a non-woven fabric seedling raising bag, wherein the height of the garden soil is 35% of that of the non-woven fabric seedling raising bag, then uniformly scattering sand and the seeds collected in the step S1 in a weight ratio of 1200:1 into the non-woven fabric seedling raising bag, then covering sand on the surface, wherein the sand covering height is 2-3mm, transferring the non-woven fabric seedling raising bag into a water containing device for culture, and the height of the water containing device is 15cm higher than that of a culture system in the non-woven fabric seedling raising bag;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Comparative example 2
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, the seeds collected in the step S1 are uniformly sown, sand is coated on the surface of the seeds, the sand coating height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2.5 percent;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Comparative example 3
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2.5 percent;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, the water surface in the water containing device is gradually increased according to the growth condition of the seedlings;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Test examples
1000 water lily seeds are taken for each group for germination culture, the germination rate and the seedling rate of the seeds are observed and recorded in the culture process, and the results are shown in table 1;
TABLE 1
Group of | Germination Rate (%) | Percent seedling rate (%) |
Example 1 | 98.1 | 87.8 |
Example 2 | 95.9 | 83.7 |
Example 3 | 96.5 | 86.4 |
Example 4 | 93.7 | 81.2 |
Comparative example 1 | 81.4 | 69.4 |
Comparative example 2 | 77.6 | 65.7 |
Comparative example 3 | 96.2 | 70.4 |
According to experimental data, the water lily cultured by the method disclosed by the invention can effectively promote the germination of water lily seeds and improve the seed germination rate and seedling survival rate.
Compared with the example 1, the water lily seedlings cultured by the tissue culture method have low seedling rate because the activated sludge is not used as the culture substrate in the comparative example 1.
Compared with the example 1, the sand is not used in the comparative example 2, and the germination rate and the seedling rate of the water lily seedlings cultivated in the group are low.
In comparative example 3, the water level was increased instead of decreasing after the seeds were germinated, and both mosquito larvae and algae in the water affected the survival of the seedlings, resulting in a low seedling survival rate, compared to example 1.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. The efficient water lily seed germination method is characterized by comprising the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge, putting the mixture into a non-woven fabric seedling bag, mixing sand with the seeds collected in the step S1, then broadcasting the mixture into the non-woven fabric seedling bag, covering the non-woven fabric seedling bag with the sand, and transferring the non-woven fabric seedling bag into a water holding device for culture; garden soil, activated sludge, seeds and sand in the non-woven fabric seedling raising bags form a culture system;
s3, seedling management: placing the water containing device in a scattered light environment at 20-30 ℃; in the initial stage of culture, the water level of the water containing device is controlled to be higher than the culture system in the non-woven fabric seedling raising bag; after the seeds germinate, controlling the water surface of the water containing device to be lower than or equal to the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
2. The efficient water lily seed germination method according to claim 1, wherein the weight ratio of garden soil to activated sludge is 5-9: 1.
3. the method for efficiently germinating the water lily seeds as claimed in claim 1 or 2, wherein the activated sludge is humus sludge with a solid content of 1.5-2%.
4. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the seeds and the sand are mixed according to the weight ratio of 1: 1000-1500.
5. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the thickness of the sand coating is 2-3 mm.
6. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the height of the water holding device is more than 15cm higher than the culture system in the non-woven fabric seedling raising bag.
7. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the illumination intensity of the scattered light is 2000Lux-2500 Lux.
8. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the water level of the water holding control device is 1-6cm higher than the culture system in the non-woven fabric seedling raising bag.
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