CN113348987A - Efficient germination method for water lily seeds - Google Patents

Efficient germination method for water lily seeds Download PDF

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Publication number
CN113348987A
CN113348987A CN202110796676.6A CN202110796676A CN113348987A CN 113348987 A CN113348987 A CN 113348987A CN 202110796676 A CN202110796676 A CN 202110796676A CN 113348987 A CN113348987 A CN 113348987A
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seeds
water
woven fabric
water lily
seedling
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陈金花
朱天龙
杨光穗
黄素荣
谌振
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Hainan Fudu Lianyuan Ecological Agriculture Co ltd
Tropical Crops Genetic Resources Institute CATAS
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Hainan Fudu Lianyuan Ecological Agriculture Co ltd
Tropical Crops Genetic Resources Institute CATAS
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Publication of CN113348987A publication Critical patent/CN113348987A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention provides a water lily seed high-efficiency germination method, which is characterized in that garden soil and activated sludge are placed at the bottom layer of a non-woven fabric seedling bag, then a mixture of seeds and sand is placed, the water level is timely reduced after the seeds germinate, and the seeds are prevented from directly contacting the activated sludge and the external environment by utilizing the physical isolation effect of the sand, so that the problem that the water quality requirement of the water lily sowing and breeding technology is high is solved in a breakthrough manner, and the current situation that the seedling rate is low due to the influence of mosquito larvae, algae and the like after the seeds germinate is effectively solved; by adopting the germination method, the germination rate of the water lily can be improved, and the seedling rate can be improved to more than 80%; the method has the advantages of high seedling rate, low cost and simple operation, and the seeds and the soil do not need to be sterilized and can be widely applied to field or indoor seedling culture.

Description

Efficient germination method for water lily seeds
Technical Field
The invention relates to the technical field of aquatic plant cultivation, in particular to a high-efficiency germination method of water lily seeds.
Background
Nymphaea spp is a perennial aquatic plant of Nymphaeaceae (Nymphaeaceae) Nymphaeaceae, and is widely applied to cut flowers, potted flowers, water landscape construction and ecological restoration engineering due to beautiful flower types, rich flower colors and unique flavor. Meanwhile, the water lily is called as beauty sleeping in flowers, is pursued by gardening enthusiasts all over the world and is a favorite for aquatic flower exhibition at home and abroad. More than 50 kinds of water lily original strains exist in the world, are mainly distributed in tropical, subtropical and temperate regions, and are originally produced in 5 kinds in China. At present, after long-term breeding by breeders in various countries, thousands of water lily existing varieties are obtained.
The water lily propagation method comprises seed propagation, plant division propagation and tissue culture rapid propagation, but the tissue culture rapid propagation technology is not popularized and applied due to the difficulties in explant disinfection, callus induction and the like. In addition, the propagation coefficient of the plant division propagation is low, and the seed propagation technology is the most widely applied technology in production and breeding work at present. However, the seedlings of water lily in the planting and breeding process are weak in the initial stage, are easily affected by external factors such as illumination, water quality, microorganisms and soil nutrition, and are easily damaged by mosquitoes and chironomid larvae.
The invention patent (patent number CN103461134A) of Chinese invention discloses a method for raising the seedling rate to 80% by improving the germination method, but the method has higher requirement on the cultivation environment of water lily.
The utility model discloses a chinese utility model patent (patent number CN210694847U) a royal lotus, high-efficient germination apparatus of water lily seed discloses and improves the germination rate through improving seed treatment method and germination apparatus, and nevertheless the water lily seedling initial stage is more delicate, and when placing natural environment with the water lily seedling that will adopt the device to cultivate, receives external factor's influence easily, and water lily seedling survival rate is low.
In order to solve the series of problems, the invention overcomes the defects of the prior art, invents a seedling raising technology with high germination rate and seedling rate, simple operation and low cost, does not need to carry out sterile treatment on seeds and a matrix, realizes the seedling raising rate of the water lily seeds by propagating to be more than 80% in a breakthrough way, can effectively promote the conservation of endangered species of the water lily, and is widely applied to crossbreeding, production and propagation.
Disclosure of Invention
Aiming at the problems, the invention provides a high-efficiency germination method of water lily seeds.
The invention discloses a high-efficiency germination method of water lily seeds, which comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds for later use;
s2, seedling culture: mixing garden soil and activated sludge, putting the mixture into a non-woven fabric seedling raising bag, mixing sand with the seeds collected in the step S1, uniformly scattering the mixture into the non-woven fabric seedling raising bag, covering the surface with the sand, and transferring the non-woven fabric seedling raising bag into a water holding device for culture; garden soil, activated sludge, seeds and sand in the non-woven fabric seedling raising bags form a culture system;
s3, seedling management: placing the water containing device in a scattered light environment at 20-30 ℃; in the initial stage of culture, the water level of the water containing device is controlled to be higher than the culture system in the non-woven fabric seedling raising bag; after the seeds germinate, controlling the water surface of the water containing device to be lower than or equal to the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Furthermore, the height of the mixture of the garden soil and the activated sludge is 30-50% of the height of the non-woven fabric seedling raising bag.
Further, the weight ratio of the garden soil to the activated sludge is 5-9: 1.
furthermore, the activated sludge is humic sludge with the solid content of 2-4%, the sludge is mixed with garden soil according to a proportion, sufficient nutrients can be provided for seeds, the germination rate of the seeds is improved, and if the solid content is too low, water quality can be polluted during germination culture, and the water lily seed development is not facilitated.
Furthermore, the non-woven fabric seedling raising bag is a 20-18 non-woven fabric seedling raising bag.
Further, the seeds and sand were mixed in a weight ratio of 1: 1000-1500.
Further, the thickness of the sand coating is 2-3 mm.
Furthermore, the height of the water containing device is more than 15cm higher than the culture system in the non-woven fabric seedling raising bag.
Further, the illumination intensity of the scattered light is 2000Lux-2500Lux, and the illumination intensity is suitable for the germination of the water lily seeds.
Furthermore, the water level of the water containing control device is 1-6cm higher than the culture system in the non-woven fabric seedling raising bag.
Further, the water containing device includes, but is not limited to, a basin, a tank, and a pool.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, garden soil and activated sludge are placed on the bottom layer of the non-woven fabric seedling raising bag, and then the mixture of seeds and sand is placed, so that the water level is timely reduced after the seeds germinate, and the physical isolation effect of the sand is utilized to prevent the seeds from directly contacting the activated sludge and the external environment, so that the problem that the water quality requirement of the water lily sowing and breeding technology is high is solved in a breakthrough manner, and the current situation that the seedling rate is low due to the influence of mosquito larvae, algae and the like after the seeds germinate is effectively solved; by adopting the germination method, the germination rate of the water lily can be improved, and the seedling rate can be improved to more than 80%; the method has the advantages of high seedling rate, low cost and simple operation, and the seeds and the soil do not need to be sterilized and can be widely applied to field or indoor seedling culture.
According to the invention, sand and seeds are mixed, and the proportion of the sand and the seeds is adjusted, so that the seeds can fully absorb nutrients in the bottom garden soil and the activated sludge under the condition of physical isolation from the outside.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The culture system is an integral body formed by garden soil, activated sludge, seeds and sand in a non-woven fabric seedling raising bag after broadcasting and sand covering.
Example 1
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2.5 percent;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Example 2
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 5: 1, the mixture of garden soil and activated sludge is 50% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1000:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 18cm higher than that of a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2%;
s3, seedling management: placing the water containing device in an environment with the temperature of 20 ℃ and the illumination intensity of scattered light of 2500 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 1cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be level with the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Example 3
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 4%;
s3, seedling management: placing the water containing device in an environment with the temperature of 23 ℃ and the intensity of scattered light illumination of 2000 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Example 4
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and return sludge according to the proportion of 9: 1, the height of the mixture of garden soil and return sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the height of the covered sand is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than or equal to the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Comparative example 1
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: putting garden soil into a non-woven fabric seedling raising bag, wherein the height of the garden soil is 35% of that of the non-woven fabric seedling raising bag, then uniformly scattering sand and the seeds collected in the step S1 in a weight ratio of 1200:1 into the non-woven fabric seedling raising bag, then covering sand on the surface, wherein the sand covering height is 2-3mm, transferring the non-woven fabric seedling raising bag into a water containing device for culture, and the height of the water containing device is 15cm higher than that of a culture system in the non-woven fabric seedling raising bag;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Comparative example 2
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, the seeds collected in the step S1 are uniformly sown, sand is coated on the surface of the seeds, the sand coating height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2.5 percent;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, controlling the water surface of the water containing device to be lower than the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Comparative example 3
A high-efficiency germination method of water lily seeds comprises the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge according to the proportion of 9: 1, the mixture of garden soil and activated sludge is 35% of the height of the non-woven fabric seedling bag, sand and the seeds collected in the step S1 are uniformly scattered in the non-woven fabric seedling bag after being mixed according to the weight ratio of 1200:1, then the surface of the non-woven fabric seedling bag is covered with sand, the sand covering height is 2-3mm, the non-woven fabric seedling bag is transferred to a water containing device for culture, and the height of the water containing device is 15cm higher than a culture system in the non-woven fabric seedling bag; the activated sludge is humic sludge with a solid content of 2.5 percent;
s3, seedling management: placing the water containing device in an environment with the temperature of 27 ℃ and the intensity of scattered light illumination of 2300 Lux; in the initial stage of culture, controlling the water level in the water containing device to be 4cm higher than that of the culture system; after the seeds germinate, the water surface in the water containing device is gradually increased according to the growth condition of the seedlings;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
Test examples
1000 water lily seeds are taken for each group for germination culture, the germination rate and the seedling rate of the seeds are observed and recorded in the culture process, and the results are shown in table 1;
TABLE 1
Group of Germination Rate (%) Percent seedling rate (%)
Example 1 98.1 87.8
Example 2 95.9 83.7
Example 3 96.5 86.4
Example 4 93.7 81.2
Comparative example 1 81.4 69.4
Comparative example 2 77.6 65.7
Comparative example 3 96.2 70.4
According to experimental data, the water lily cultured by the method disclosed by the invention can effectively promote the germination of water lily seeds and improve the seed germination rate and seedling survival rate.
Compared with the example 1, the water lily seedlings cultured by the tissue culture method have low seedling rate because the activated sludge is not used as the culture substrate in the comparative example 1.
Compared with the example 1, the sand is not used in the comparative example 2, and the germination rate and the seedling rate of the water lily seedlings cultivated in the group are low.
In comparative example 3, the water level was increased instead of decreasing after the seeds were germinated, and both mosquito larvae and algae in the water affected the survival of the seedlings, resulting in a low seedling survival rate, compared to example 1.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (8)

1. The efficient water lily seed germination method is characterized by comprising the following steps:
s1, seed pretreatment: selecting water lily polymeric fruits with full shapes, collecting mature water lily seeds, and cleaning the seeds;
s2, seedling culture: mixing garden soil and activated sludge, putting the mixture into a non-woven fabric seedling bag, mixing sand with the seeds collected in the step S1, then broadcasting the mixture into the non-woven fabric seedling bag, covering the non-woven fabric seedling bag with the sand, and transferring the non-woven fabric seedling bag into a water holding device for culture; garden soil, activated sludge, seeds and sand in the non-woven fabric seedling raising bags form a culture system;
s3, seedling management: placing the water containing device in a scattered light environment at 20-30 ℃; in the initial stage of culture, the water level of the water containing device is controlled to be higher than the culture system in the non-woven fabric seedling raising bag; after the seeds germinate, controlling the water surface of the water containing device to be lower than or equal to the highest point of the culture system;
s4, transplanting: transplanting after the 3 rd to 4 th true leaves of the water lily seedlings grow.
2. The efficient water lily seed germination method according to claim 1, wherein the weight ratio of garden soil to activated sludge is 5-9: 1.
3. the method for efficiently germinating the water lily seeds as claimed in claim 1 or 2, wherein the activated sludge is humus sludge with a solid content of 1.5-2%.
4. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the seeds and the sand are mixed according to the weight ratio of 1: 1000-1500.
5. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the thickness of the sand coating is 2-3 mm.
6. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the height of the water holding device is more than 15cm higher than the culture system in the non-woven fabric seedling raising bag.
7. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the illumination intensity of the scattered light is 2000Lux-2500 Lux.
8. The method for efficiently germinating the water lily seeds as claimed in claim 1, wherein the water level of the water holding control device is 1-6cm higher than the culture system in the non-woven fabric seedling raising bag.
CN202110796676.6A 2021-07-14 2021-07-14 Efficient germination method for water lily seeds Pending CN113348987A (en)

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